365 results on '"Cai J"'
Search Results
2. Maximizing blue carbon stocks through saltmarsh restoration
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Lucy McMahon, Cai J. T. Ladd, Annette Burden, Ed Garrett, Kelly R. Redeker, Peter Lawrence, and Roland Gehrels
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Global and Planetary Change ,Ocean Engineering ,Aquatic Science ,Oceanography ,Water Science and Technology - Abstract
Political discourse around coastal wetland restoration and blue carbon management strategies has increased in the past decade, yet carbon storage has neither been a reason for restoration, nor a criterion to measure the success of current saltmarsh restoration schemes in the UK. To maximise climate change mitigation through saltmarsh restoration, knowledge on the key drivers of carbon stock variability is required. We use restored saltmarshes of similar age, paired with adjacent natural marshes as references, to identify drivers of carbon stocks following managed realignment within an estuary in southeastern England. From surficial soil cores (top 30 cm), we measured carbon stock alongside environmental characteristics. Carbon stock between natural and restored sites were similar after ~ 30 years when restored sites were above mean high water neap (MHWN) tidal levels. Elevated marsh platforms likely provide suitable conditions for the development of mature plant communities associated with greater capture and production of organic carbon. The restored site at Tollesbury (Essex, UK) had a 2-fold lower carbon stock than other restored sites in the estuary. We attribute this to the site’s low position in the tidal frame, below MHWN tidal levels, coupled with low sediment supply and the dominance of pioneer plant communities. As blue carbon is anticipated to become an important facet of saltmarsh restoration, we recommend that sites above MHWN tidal levels are selected for managed realignment or that preference is given to coastlines with a high sediment supply that may rapidly elevate realignment sites above MHWN. Alternatively, elevation could be artificially raised prior to realignment. Restoration schemes aiming to maximise climate change mitigation should also encourage the establishment of key plant species (e.g., Atriplex portulacoides in our study) to enhance carbon stocks. However, the overall goal of restoration ought to be carefully considered as trade-offs in ecosystem services may ensue if restoration for climate change mitigation alone is pursued.
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- 2023
3. MACHINE LEARNING-BASED ECONOMIC DEVELOPMENT MAPPING FROM MULTI-SOURCE OPEN GEOSPATIAL DATA
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Cao, R., Tu, W., Cai, J., Zhao, T., Xiao, J., Cao, J., Gao, Q., and Su, H.
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Timely and accurate socioeconomic indicators are the prerequisite for smart social governance. For example, the level of economic development and the structure of population are important statistics for regional or national policy-making. However, the collection of these characteristics usually depends on demographic and social surveys, which are time- and labor-intensive. To address these issues, we propose a machine learning-based approach to estimate and map the economic development from multi-source open available geospatial data, including remote sensing imagery and OpenStreetMap road networks. Specifically, we first extract knowledge-based features from different data sources; then the multi-view graphs are constructed through different perspectives of spatial adjacency and feature similarity; and a multi-view graph neural network (MVGNN) model is built on them and trained in a self-supervised learning manner. Then, the handcrafted features and the learned graph representations are combined to estimate the regional economic development indicators via random forest models. Taking China’s county-level gross domestic product (GDP) as an example, extensive experiments have been conducted and the results demonstrate the effectiveness of the proposed method, and the combination of the knowledge-based and learning-based features can significantly outperform baseline methods. Our proposed approach can advance the goal of acquiring timely and accurate socioeconomic variables through widely accessible geospatial data, which has the potential to extend to more social indicators and other geographic regions to support smart governance and policy-making in the future.
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- 2022
4. The First LHAASO Catalog of Gamma-Ray Sources
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Cao, Zhen, Aharonian, F., An, Q., Axikegu, Bai, Y. X., Bao, Y. W., Bastieri, D., Bi, X. J., Bi, Y. J., Cai, J. T., Cao, Q., Cao, W. Y., Cao, Zhe, Chang, J., Chang, J. F., Chen, A. M., Chen, E. S., Chen, Liang, Chen, Lin, Chen, Long, Chen, M. J., Chen, M. L., Chen, Q. H., Chen, S. H., Chen, S. Z., Chen, T. L., Chen, Y., Cheng, N., Cheng, Y. D., Cui, M. Y., Cui, S. W., Cui, X. H., Cui, Y. D., Dai, B. Z., Dai, H. L., Dai, Z. G., Danzengluobu, della Volpe, D., Dong, X. Q., Duan, K. K., Fan, J. H., Fan, Y. Z., Fang, J., Fang, K., Feng, C. F., Feng, L., Feng, S. H., Feng, X. T., Feng, Y. L., Gabici, S., Gao, B., Gao, C. D., Gao, L. Q., Gao, Q., Gao, W., Gao, W. K., Ge, M. M., Geng, L. S., Giacinti, G., Gong, G. H., Gou, Q. B., Gu, M. H., Guo, F. L., Guo, X. L., Guo, Y. Q., Guo, Y. Y., Han, Y. A., He, H. H., He, H. N., He, J. Y., He, X. B., He, Y., Heller, M., Hor, Y. K., Hou, B. W., Hou, C., Hou, X., Hu, H. B., Hu, Q., Hu, S. C., Huang, D. H., Huang, T. Q., Huang, W. J., Huang, X. T., Huang, X. Y., Huang, Y., Huang, Z. C., Ji, X. L., Jia, H. Y., Jia, K., Jiang, K., Jiang, X. W., Jiang, Z. J., Jin, M., Kang, M. M., Ke, T., Kuleshov, D., Kurinov, K., Li, B. B., Li, Cheng, Li, Cong, Li, D., Li, F., Li, H. B., Li, H. C., Li, H. Y., Li, J., Li, Jian, Li, Jie, Li, K., Li, W. L., Li, X. R., Li, Xin, Li, Y. Z., Li, Zhe, Li, Zhuo, Liang, E. W., Liang, Y. F., Lin, S. J., Liu, B., Liu, C., Liu, D., Liu, H., Liu, H. D., Liu, J., Liu, J. L., Liu, J. Y., Liu, M. Y., Liu, R. Y., Liu, S. M., Liu, W., Liu, Y., Liu, Y. N., Lu, R., Luo, Q., Lv, H. K., Ma, B. Q., Ma, L. L., Ma, X. H., Mao, J. R., Min, Z., Mitthumsiri, W., Mu, H. J., Nan, Y. C., Neronov, A., Ou, Z. W., Pang, B. Y., Pattarakijwanich, P., Pei, Z. Y., Qi, M. Y., Qi, Y. Q., Qiao, B. Q., Qin, J. J., Ruffolo, D., Sáiz, A., Semikoz, D., Shao, C. Y., Shao, L., Shchegolev, O., Sheng, X. D., Shu, F. W., Song, H. C., Stenkin, Yu. V., Stepanov, V., Su, Y., Sun, Q. N., Sun, X. N., Sun, Z. B., Tam, P. H. T., Tang, Q. W., Tang, Z. B., Tian, W. W., Wang, C., Wang, C. B., Wang, G. W., Wang, H. G., Wang, H. H., Wang, J. C., Wang, K., Wang, L. P., Wang, L. Y., Wang, P. H., Wang, R., Wang, W., Wang, X. G., Wang, X. Y., Wang, Y., Wang, Y. D., Wang, Y. J., Wang, Z. H., Wang, Z. X., Wang, Zhen, Wang, Zheng, Wei, D. M., Wei, J. J., Wei, Y. J., Wen, T., Wu, C. Y., Wu, H. R., Wu, S., Wu, X. F., Wu, Y. S., Xi, S. Q., Xia, J., Xia, J. J., Xiang, G. M., Xiao, D. X., Xiao, G., Xin, G. G., Xin, Y. L., Xing, Y., Xiong, Z., Xu, D. L., Xu, R. F., Xu, R. X., Xu, W. L., Xue, L., Yan, D. H., Yan, J. Z., Yan, T., Yang, C. W., Yang, F., Yang, F. F., Yang, H. W., Yang, J. Y., Yang, L. L., Yang, M. J., Yang, R. Z., Yang, S. B., Yao, Y. H., Yao, Z. G., Ye, Y. M., Yin, L. Q., Yin, N., You, X. H., You, Z. Y., Yu, Y. H., Yuan, Q., Yue, H., Zeng, H. D., Zeng, T. X., Zeng, W., Zha, M., Zhang, B. B., Zhang, F., Zhang, H. M., Zhang, H. Y., Zhang, J. L., Zhang, L. X., Zhang, Li, Zhang, P. F., Zhang, P. P., Zhang, R., Zhang, S. B., Zhang, S. R., Zhang, S. S., Zhang, X., Zhang, X. P., Zhang, Y. F., Zhang, Yi, Zhang, Yong, Zhao, B., Zhao, J., Zhao, L., Zhao, L. Z., Zhao, S. P., Zheng, F., Zhou, B., Zhou, H., Zhou, J. N., Zhou, M., Zhou, P., Zhou, R., Zhou, X. X., Zhu, C. G., Zhu, F. R., Zhu, H., Zhu, K. J., and Zuo., X.
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High Energy Astrophysical Phenomena (astro-ph.HE) ,High Energy Physics - Phenomenology ,High Energy Physics - Phenomenology (hep-ph) ,FOS: Physical sciences ,Astrophysics - High Energy Astrophysical Phenomena - Abstract
We present the first catalog of very-high energy and ultra-high energy $\gamma$-ray sources detected by the Large High Altitude Air Shower Observatory (LHAASO), using 508 days of data collected by the Water Cherenkov Detector Array (WCDA) from March 2021 to September 2022 and 933 days of data recorded by the Kilometer Squared Array (KM2A) from January 2020 to September 2022. This catalog represents the most sensitive $E > 1$ TeV gamma-ray survey of the sky covering declination from $-$20$^{\circ}$ to 80$^{\circ}$. In total, the catalog contains 90 sources with extended size smaller than $2^\circ$ and with significance of detection at $> 5\sigma$. For each source, we provide its position, extension and spectral characteristics. Furthermore, based on our source association criteria, 32 new TeV sources are proposed in this study. Additionally, 43 sources are detected with ultra-high energy ($E > 100$ TeV) emission at $> 4\sigma$ significance level., Comment: 35 pages, 10 figures, 3 tables
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- 2023
- Full Text
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5. A Newly Defined Pyroptosis-Related Gene Signature for the Prognosis of Bladder Cancer
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Chen W, Zhang W, Zhou T, Cai J, Yu Z, and Wu Z
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Medicine (General) ,R5-920 ,pyroptosis ,bladder cancer ,prognosis ,tcga ,immune - Abstract
Weikang Chen,1,* Wenhao Zhang,2,3,* Tao Zhou,1,* Jian Cai,1 Zhixian Yu,1 Zhigang Wu1 1Department of Urology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325000, People’s Republic of China; 2The Fourth School of Clinical Medicine, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 325000, People’s Republic of China; 3Department of Urology, Affiliated Hangzhou First People’s Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 325000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhixian Yu; Zhigang WuDepartment of Urology, The First Affiliated Hospital of Wenzhou Medical University, No. 2, Fuxue Road Lucheng District, Wenzhou, Zhejiang, 325000, People’s Republic of ChinaEmail yuzx515@163.com; andrologywzg@wmu.edu.cnBackground: Bladder cancer (BC), as the most common urinary system tumor type and the main cause of tumor-related death, has an unsatisfactory prognosis. In recent years, related literature has proposed that cell pyroptosis is an inflammatory form of programmed cell death. However, in BC, the relationship between the expression of pyroptosis-related genes and the prognosis has not been elucidated.Methods: We got the RNA sequencing data from TCGA and GEO datasets. Fifty-two pyroptosis-related genes were extracted for further explore. Then, we compared the gene expression levels between the normal bladder and BC tissues. After that, we develop and validate a pyroptosis-related gene prognostic model and made following functional enrichment analysis and single-sample gene set enrichment analysis of the differentially expressed genes between the high- and low-risk groups.Results: Twenty-nine differentially expressed genes (DEGs) were found between normal and tumor tissues. Based on the median score calculated by the risk score formula from 8 pyroptosis-related genes, 414 patients were equally divided into low- and high-risk subgroups. The survival probability of BC patients in the high-risk group was significantly lower than that in the low-risk group (P < 0.001). Through multivariate analysis, our risk score is an independent factor predicting OS in BC patients. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis show that high-risk populations are rich in immune-related genes and have a decreased immune status. All the above results have been externally verified from GEO cohort.Conclusion: Pyroptosis-related genes are closely related to tumor immunity and are a potential prognostic tool for predicting BCs.Keywords: pyroptosis, bladder cancer, immune, prognosis, TCGA
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- 2021
6. Newly-Diagnosed Diabetes and Sustained Hyperglycemia are Associated with Poorer Outcomes in COVID-19 Inpatients Without Pre-Existing Diabetes
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Lin L, Chen Z, Ding T, Liu H, Zhou F, Huang X, Zhang X, Liu W, Zhang BH, Yuan Y, Zhang P, Zhang XJ, She ZG, Cai J, Chen W, and Li H
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covid-19 ,RC581-951 ,blood glucose ,Specialties of internal medicine ,newly diagnosed diabetes ,hyperglycemia ,prognosis - Abstract
Lijin Lin,1– 4,* Ze Chen,4,5,* Ting Ding,1,2,* Hui Liu,4,6,* Feng Zhou,4,7 Xuewei Huang,3,4 Xingyuan Zhang,4,8 Weifang Liu,4,8 Bing-Hong Zhang,9 Yufeng Yuan,10 Peng Zhang,4,8 Xiao-Jing Zhang,4,8 Zhi-Gang She,3,4 Jingjing Cai,4,11 Wenping Chen,1,2 Hongliang Li2– 4,7 1Department of Endocrinology, Huanggang Central Hospital, Huanggang, People’s Republic of China; 2Huanggang Institute of Translational Medicine, Huanggang, People’s Republic of China; 3Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, People’s Republic of China; 4Institute of Model Animal of Wuhan University, Wuhan, People’s Republic of China; 5Department of Cardiology, Zhongnan Hospital of Wuhan University, Wuhan, People’s Republic of China; 6Neonatology of Gastroenterology, Tongren Hospital of Wuhan University & Wuhan Third Hospital, Wuhan, People’s Republic of China; 7Medical Science Research Center, Zhongnan Hospital of Wuhan University, Wuhan, People’s Republic of China; 8Basic Medical School of Wuhan University, Wuhan, People’s Republic of China; 9Neonatology Department, Renmin Hospital of Wuhan University, Wuhan, People’s Republic of China; 10Department of Hepatobiliary and Pancreatic Surgery, Zhongnan Hospital of Wuhan University, Wuhan, People’s Republic of China; 11Department of Cardiology, The Third Xiangya Hospital, Central South University, Changsha, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hongliang Li; Wenping Chen Email lihl@whu.edu.cn; chenwenping@hgyy.org.cnPurpose: To analyze the impact of hyperglycemia on the clinical outcome of COVID-19 in patients with newly diagnosed diabetes (NDD).Patients and Methods: We performed a retrospective study of 3114 cases of COVID-19 without pre-existing diabetes, 351 of which had NDD, in Hubei Province, China. The Cox regression model was used to calculate the risk of adverse clinical outcomes comparing the NDD vs non-NDD group before and after propensity score-matched (PSM) analysis. Patients with NDD were further divided into a sustained hyperglycemia group, a fluctuating group, and a remitted group based on their blood glucose levels during hospitalization as well as into hypoglycemic agent users and nonusers.Results: Compared to the non-NDD individuals, individuals with NDD had a significantly increased risk of all-cause mortality (adjusted HR after PSM, 2.65; 95% CI, 1.49– 4.72; P = 0.001) and secondary outcomes involving organ damage during the 28-day follow-up period. Subgroup analyses indicated that among individuals with NDD, the individuals with remitted hyperglycemia had the lowest 28-day mortality, whereas those with sustained hyperglycemia had the highest (IRR 24.27; 95% CI, 3.21– 183.36; P < 0.001). Moreover, individuals treated with hypoglycemic agents had significantly lower all-cause mortality than those not treated with hypoglycemic agents (IRR 0.08; 95% CI, 0.01– 0.56; P < 0.001).Conclusion: Our study reinforces the clinical message that NDD is strongly associated with poor outcomes in COVID-19 patients. Furthermore, resolved hyperglycemia in the later phase of the disease and the use of hypoglycemic agents were associated with improved prognosis in patients with NDD.Keywords: COVID-19, newly diagnosed diabetes, hyperglycemia, prognosis, blood glucose
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- 2021
7. Erector Spinae Plane Block for Perioperative Pain Control and Short-term Outcomes in Lumbar Laminoplasty: A Randomized Clinical Trial
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Jin Y, Zhao S, Cai J, Blessing M, Zhao X, Tan H, and Li J
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Medicine (General) ,erector spinae plane block ,R5-920 ,perioperative pain ,opioid ,laminoplasty ,short-term outcome ,spine - Abstract
Yanwu Jin,1 Shanshan Zhao,1 Jiahui Cai,2 Marcelle Blessing,3 Xin Zhao,1 Haizhu Tan,2 Jinlei Li3 1Department of Anesthesiology, Second Hospital of Shandong University, Shandong University, Jinan, Shandong, People’s Republic of China; 2Department of Preventive Medicine, Shantou University Medical College, Shantou, Guangdong, People’s Republic of China; 3Department of Anesthesiology, Yale University School of Medicine, New Haven, CT, USACorrespondence: Jinlei Li; Haizhu TanDepartment of Anesthesiology, Yale University School of Medicine, 333 Cedar Street, TMP 3, New Haven, CT, 06520-8051, USATel +1 203 785-2802Fax +1 203 785-6664Email jinlei.li@yale.edu; linnanqia@126.comBackground: Spine surgery causes severe pain and can be associated with significant opioid utilization; however, the evidence for opioid-sparing analgesic techniques such as erector spinae plane (ESP) block from controlled studies is limited. We aimed to investigate the analgesic effects of ESP block in lumbar laminoplasty.Methods: In this prospective, double-blind, controlled single-center trial, 62 consecutive elective lumbar laminoplasty patients were randomized into either a control group (Group G, N=32) or a treatment group (Group E, N=30). Group G received general anesthesia and multimodal analgesia, similar to group E, while Group E received additional bilateral ESP block after induction of general anesthesia. The primary outcome was postoperative pain scores for the first 48 h after surgery, and the secondary outcomes analyzed included intraoperative anesthetic usage, perioperative analgesic consumption, return of bowel function and satisfaction for acute pain management indicated by overall benefit of analgesia score (OBAS).Results: Significant differences in pain scores over time were found between the two groups (P=0.010), with Group E patients having significantly lower pain scores than Group G during the first six hours (P=0.000). The opioid consumption in Group G was significantly higher than in Group E both intraoperatively (P=0.000) and postoperatively (P=0.0005). Group E patients had lower intraoperative sevoflurane requirement, improved satisfaction with pain management, and earlier return of bowel function than Group G patients.Conclusion: ESP block is effective in reducing postoperative pain scores and lowering opioid utilization (both intraoperatively and postoperatively), resulting in improved patient satisfaction for pain management in lumbar laminoplasty.Keywords: erector spinae plane block, laminoplasty, spine, perioperative pain, opioid, short-term outcome
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- 2021
8. Inhibition of MALT1 Alleviates Spinal Ischemia/Reperfusion Injury-Induced Neuroinflammation by Modulating Glial Endoplasmic Reticulum Stress in Rats
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Zhang S, Yan Y, Wang Y, Sun Z, Han C, Qian X, Ren X, Feng Y, Cai J, and Xia C
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spinal cord ischemia/reperfusion injury ,blood-spinal cord barrier ,malt1 ,astrocytes ,endoplasmic reticulum stress ,Pathology ,microglia ,RB1-214 ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Shutian Zhang,1,2,* Yufeng Yan,3,* Yongze Wang,1,2 Zhaodong Sun,1,2 Chengzhi Han,1,2 Xinyi Qian,1,2 Xiaorong Ren,1,2 Yi Feng,4 Jian Cai,5 Chunmei Xia1 1Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, People’s Republic of China; 2Department of Clinical Medicine, Shanghai Medical College, Fudan University, Shanghai, 200032, People’s Republic of China; 3Experimental Teaching Center of Basic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, People’s Republic of China; 4Department of Integrative Medicine and Neurobiology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, People’s Republic of China; 5Department of Neurology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200240, People’s Republic of China*These authors contributed equally to this workCorrespondence: Chunmei XiaDepartment of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, People’s Republic of ChinaTel +86 21 54237612-805Email cmxia@fudan.edu.cnJian CaiDepartment of Neurology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200240, People’s Republic of ChinaEmail feele@sina.comPurpose: Glial activation and the disorders of cytokine secretion induced by endoplasmic reticulum stress (ERS) are crucial pathogenic processes in establishing ischemia/reperfusion (I/R) injury of the brain and spinal cord. This present study aimed to investigate the effects of mucous-associated lymphoid tissue lymphoma translocation protein 1 (MALT1) on spinal cord ischemia/reperfusion (SCI/R) injury via regulating glial ERS.Methods: SCI/R was induced by thoracic aorta occlusion-reperfusion in rats. The MALT1-specific inhibitor MI-2 or human recombinant MALT1 protein (hrMALT1) was administrated for three consecutive days after the surgery. Immunofluorescent staining was used to detect the localization of MALT1 and ERS profiles in activated astrocyte and microglia of spinal cord. The ultrastructure of endoplasmic reticulum (ER) was examined by transmission electron microscopy. Blood-spinal cord barrier (BSCB) disruption and noninflammatory status were assessed. The neuron loss and demyelination in the spinal cord were monitored, and the hindlimb motor function was evaluated in SCI/R rats.Results: Intraperitoneally postoperative MI-2 treatment down-regulated phos-NF-κB (p65) and Bip (ERS marker protein) expression in the spinal cord after SCI/R in rats. Intraperitoneal injection MI-2 attenuated the swelling/dilation of ER of the glia in SCI/R rats. Furthermore, MI-2 attenuated I/R-induced Evans blue (EB) leakage and microglia M1 polarization in spinal cord, implying a role for MALT1 in the BSCB destruction and neuroinflammation after SCI/R in rats. Furthermore, intrathecal injection of hrMALT1 aggravated the fragmentation of neuron, loss of neurofibrils and demyelination caused by I/R, while 4-PBA, an ERS inhibitor, co-treatment with hrMALT1 reversed these effects in SCI/R rats. hrMALT1 administration aggravated the motor deficit index (MDI) scoring, while 4-PBA co-treatment improved SCI/R-induced motor deficits in rats.Conclusion: Inhibition of MALT1 alleviates SCI/R injury-induced neuroinflammation by modulating glial endoplasmic reticulum stress in rats.Keywords: MALT1, blood-spinal cord barrier, astrocytes, microglia, endoplasmic reticulum stress, spinal cord ischemia/reperfusion injury
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- 2021
9. Quantum state tomography via non-convex Riemannian gradient descent
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Hsu, M-C, Kuo, E-J, Yu, W-H, Cai, J-F, and Hsieh, M-H
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- 2022
10. Deep underground laboratory measurement of $^{13}$C($\alpha$,$n$)$^{16}$O in the Gamow windows of the $s$- and $i$-processes
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Gao, B., Jiao, T. Y., Li, Y. T., Chen, H., Lin, W. P., An, Z., Ru, L. H., Zhang, Z. C., Tang, X. D., Wang, X. Y., Zhang, N. T., Fang, X., Xie, D. H., Fan, Y. H., Ma, L., Zhang, X., Bai, F., Wang, P., Fan, Y. X., Liu, G., Huang, H. X., Wu, Q., Zhu, Y. B., Chai, J. L., Li, J. Q., Sun, L. T., Wang, S., Cai, J. W., Li, Y. Z., Su, J., Zhang, H., Li, Z. H., Li, Y. J., Li, E. T., Chen, C., Shen, Y. P., Lian, G., Guo, B., Li, X. Y., Zhang, L. Y., He, J. J., Sheng, Y. D., Chen, Y. J., Wang, L. H., Zhang, L., Cao, F. Q., Nan, W., Nan, W. K., Li, G. X., Song, N., Cui, B. Q., Chen, L. H., Ma, R. G., Yan, S. Q., Liao, J. H., Wang, Y. B., Zeng, S., Nan, D., Fan, Q. W., Qi, N. C., Sun, W. L., Guo, X. Y., Zhang, P., Chen, Y. H., Zhou, Y., Zhou, J. F., He, J. R., Shang, C. S., Li, M. C., Kubono, S., Liu, W. P., deBoer, R. J., Wiescher, M., and Pignatari, M.
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Nuclear Experiment - Abstract
The $^{13}$C($\alpha$,$n$)$^{16}$O reaction is the main neutron source for the slow-neutron-capture (s-) process in Asymptotic Giant Branch stars and for the intermediate (i-) process. Direct measurements at astrophysical energies in above-ground laboratories are hindered by the extremely small cross sections and vast cosmic-ray induced background. We performed the first consistent direct measurement in the range of $E_{\rm c.m.}=$0.24 MeV to 1.9 MeV using the accelerators at the China Jinping Underground Laboratory (CJPL) and Sichuan University. Our measurement covers almost the entire i-process Gamow window in which the large uncertainty of the previous experiments has been reduced from 60\% down to 15\%, eliminates the large systematic uncertainty in the extrapolation arising from the inconsistency of existing data sets, and provides a more reliable reaction rate for the studies of the s- and i-processes along with the first direct determination of the alpha strength for the near-threshold state.
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- 2022
11. $^{197}$Au($\gamma,\,xn;\,x\,=\,1\thicksim7$) Reaction Measurements using Laser-Driven Ultra-Bright Ultra-Fast Bremsstrahlung $\gamma$-Ray
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Wu, D., Zhang, J. Y., Lan, H. Y., Liu, J. X., Lv, J. F., Lu, H. G., Wu, X. Z., Zhang, H., Geng, Y. X., Zhao, Y. Y., Xu, J. Y., Cai, J., Yu, J. Q., Lin, C., Ma, W. J., Wang, H. R., Liu, F. L., He, C. Y., Guo, B., Zhang, G. Q., Wang, N. Y., Ma, Y. G., and Yan, X. Q.
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Nuclear Experiment - Abstract
We present a new method for the measurements of photonuclear reaction flux-weighted average cross sections and isomeric yield ratios using laser-driven ultra-bright ultra-fast bremsstrahlung $\gamma$-ray, especially for photonuclear reactions with short half-life times. A 200 TW laser was used to generate stable near-monoenergetic electron beams via laser wakefield acceleration. The flux-weighted average cross sections and isomeric ratios of $^{197}$Au($\gamma,\,xn;\,x\,=\,1\thicksim7$) reactions were analyzed through activation measurements. The results showed good agreements with previous works and were compared with TALYS 1.9 calculations. The $^{197}$Au($\gamma$,\,7n)$^{190}$Au reaction cross sections were first achieved. This method offered a unique way of gaining insight into photonuclear reaction researches with short half-life times, which extremely lack experimental data.
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- 2022
12. Unified theoretical framework for mixing state of black carbon
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Wang, J., Cai, R., Liu, C., Jiang, J., Nie, W., Moteki, N., Zaveri, R., Huang, X., Ma, N., Chen, G., Wang, Z., Jin, Y., Cai, J., Zhang, Y., Chi, X., Holanda, B., Xing, J., Liu, T., Qi, X., Wang, Q., Pöhlker, C., Su, H., Cheng, Y., Wang, S., Hao, J., Andreae, M., and Ding, A.
- Abstract
Black carbon (BC) plays an important role in the climate system due to its strongwarming effect, yet the magnitude of this effect is highly uncertain due to the complex mixingstate of aerosols. Here we build a unified theoretical framework to describe BC’s mixing states,linking dynamic processes to BC coating thickness distribution, and show its self-similarity for sites in diverse environments. The size distribution of BC-containing particles is found to followan exponential pattern and is independent of BC core size. A mixing state module is establishedbased on this finding and successfully applied in global and regional models, which increases theaccuracy of aerosol climate effect estimations. Our theoretical framework can bridge the gap be-tween observation and model simulation in both mixing state description and light absorption quantification
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- 2022
13. Clinical Features, Strain Distribution, Antifungal Resistance and Prognosis of Patients with Non-albicans Candidemia: A Retrospective Observational Study
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Liu F, Zhong L, Zhou F, Zheng C, Zhang K, Cai J, Zhou H, Tang K, Dong Z, Cui W, and Zhang G
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clinical features ,risk factor ,non-albicans candidemia ,Infectious and parasitic diseases ,RC109-216 ,cross-resistance - Abstract
Fengqi Liu,1,2,* Li Zhong,1,2,* Feifei Zhou,3,* Cheng Zheng,1,4 Kai Zhang,1 Jiachang Cai,5 Hongwei Zhou,5 Kankai Tang,2 Zhaohui Dong,2 Wei Cui,1 Gensheng Zhang1 1Department of Critical Care Medicine, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310009, People’s Republic of China; 2Department of Critical Care Medicine, First Affiliated Hospital, Huzhou Teachers College, The First People’s Hospital of Huzhou, Huzhou, 313000, Zhejiang, People’s Republic of China; 3Department of Critical Care Medicine, Ningbo Medical Center, Li Huili Hospital, Ningbo, Zhejiang, 315040, People’s Republic of China; 4Department of Critical Care Medicine, Taizhou Municipal Hospital, Taizhou, Zhejiang, 318000, People’s Republic of China; 5Department of Clinical Microbiology Laboratory, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310009, People’s Republic of China*These authors contributed equally to this workCorrespondence: Gensheng Zhang; Wei CuiDepartment of Critical Care Medicine, Second Affiliated Hospital, Zhejiang University School of Medicine, cc88 Jiefang Road, Hangzhou, 310009, People’s Republic of ChinaTel +86-571-8778-3636; +86-571-8778-3551Fax +86-571- 87022776Email genshengzhang@zju.edu.cn; zricu@zju.edu.cnPurpose: Candida albicans (C. albicans) candidemia has been well reported in previous studies, while research on non-albicans Candida (NAC) bloodstream infections remains poorly explored. Therefore, the present study aimed to investigate the clinical characteristics and outcomes of patients with NAC candidemia.Patients and Methods: We recruited inpatients with candidemia from January 2013 to June 2020 in a tertiary hospital for this retrospective observational study.Results: A total of 301 patients with candidemia were recruited in the current study, including 161 (53.5%) patients with NAC candidemia. The main pathogens in NAC candidemia were Candida tropicalis (C. tropicalis) (23.9%), Candida parapsilosis (15.6%) and Candida glabrata (10.3%). Patients with NAC candidemia had more medical admissions (P=0.034), a higher percentage of hematological malignancies (P=0.007), a higher frequency of antifungal exposure (P=0.012), and more indwelling peripherally inserted central catheters (P=0.002) than those with C. albicans candidemia. In a multivariable analysis, prior antifungal exposure was independently related to NAC candidemia (adjusted odds ratio [aOR], 0.312; 95% confidence interval [CI], 0.113– 0.859). Additionally, NAC was obviously resistant to azoles, especially C. tropicalis had a high cross-resistance to azoles. However, no significant differences were noted in the mortality rates at 14 days, 28 days and 60 days between these two groups.Conclusion: NAC is dominant in candidemia, and prior antifungal exposure is an independent risk factor. Of note, although the outcomes of NAC and C. albicans candidemia are similar, drug resistance to specific azoles as well as cross-resistance frequently occurs in patients with NAC candidemia, and this drug resistance deserves attention in clinical practice and further in-depth investigation.Keywords: non-albicans candidemia, clinical features, risk factor, cross-resistance
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- 2021
14. Effects of Oil Tea on Obesity and Dyslipidemia: A Cross-Sectional Study in China
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Cai J, Liu S, Li Y, Liu Q, Xu M, Mo C, Mai T, Xu X, Tang X, Chen Q, Nong C, Lu H, He H, Tang J, Zhang J, Wei C, Tan D, Qin J, and Zhang Z
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RC581-951 ,blood lipid ,Specialties of internal medicine ,hdl-c ,abdominal obesity - Abstract
Jiansheng Cai,1,2,* Shuzhen Liu,1,* You Li,3,* Qiumei Liu,1 Min Xu,1 Chunbao Mo,3 Tingyu Mai,3 Xia Xu,1 Xu Tang,1 Quanhui Chen,1 Chuntao Nong,1 Huaxiang Lu,1,3 Haoyu He,1,4 Jiexia Tang,5 Junling Zhang,1 Chunmei Wei,1 Dechan Tan,3 Jian Qin,1 Zhiyong Zhang1,3 1Department of Environmental and Occupational Health, School of Public Health, Guangxi Medical University, Nanning, 530021, Guangxi Province, People’s Republic of China; 2Guangxi Key Laboratory of Tumor Immunology and Microenvironmental Regulation, Guilin Medical University, Guilin, People’s Republic of China; 3Department of Environmental Health and Occupational Medicine, School of Public Health, Guilin Medical University, Guilin, People’s Republic of China; 4Quality Management Department, the Affiliated Hospital of Stomatology, Guangxi Medical University, Nanning, People’s Republic of China; 5Guangxi Center for Disease Prevention and Control, Nanning, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jian Qin; Zhiyong ZhangDepartment of Environmental and Occupational Health, School of Public Health, Guangxi Medical University, Shuangyong Road No. 22, Nanning, 530021, Guangxi Province, People’s Republic of ChinaTel +86-771-5358124Fax +86-771-5358124Email qinjian@gxmu.edu.cn; rpazz@163.comBackground: Animal experiments have found that oil tea reduces body weight and improves blood lipid levels. However, the effect of oil tea on human health has not been confirmed yet. This study aims to explore the relationship between oil tea consumption and obesity and dyslipidemia.Methods: In a cross-sectional population study in Guangxi, China, a semi-quantitative questionnaire was used to investigate the oil tea consumed and food consumption frequency in adults aged 30 years and over. Anthropometric variables and serum biochemical indicators were measured. A total of 2001 adults were divided into five groups based on their non-consumption status and quartile of consumption (groups non-drink oil tea, Q1–Q4).Results: The risk of abdominal obesity tended to decrease significantly with increasing consumption of oil tea (P for trend< 0.05) in the overall participants (Q3 group, OR = 0.545, 95% CI = 0.336– 0.884; Q4 group, OR = 0.520, 95% CI = 0.311– 0.871) and in women (Q2 group, OR = 0.502, 95% CI = 0.274– 0.920; Q3 group, OR = 0.397, 95% CI = 0.213– 0.740; Q4 group, OR = 0.421, 95% CI = 0.228– 0.780). Oil tea consumption Q1, Q2, Q3 and Q4 group significantly reduced the risk of abnormal HDL-cholesterol (P < 0.05). Oil tea consumption Q2 group significantly increased the risk of abnormal LDL-cholesterol (OR = 2.600, 95% CI = 1.033– 6.546) in women. Oil tea consumption Q1 (OR = 0.081, 95% CI =0.008– 0.864) and Q3 (OR = 0.057, 95% CI = 0.004– 0.913) groups significantly reduced the risk of abnormal HDL-cholesterol in women.Conclusion: Oil tea consumption may be associated with a low risk of abdominal obesity. High-dose oil tea consumption may be associated with a low risk of abnormal HDL-cholesterol. Prospective studies with large sample sizes would be required to further investigate this association.Keywords: abdominal obesity, blood lipid, HDL-C
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- 2021
15. Neurite Extension and Orientation of Spiral Ganglion Neurons Can Be Directed by Superparamagnetic Iron Oxide Nanoparticles in a Magnetic Field
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Hu Y, Li D, Wei H, Zhou S, Chen W, Yan X, Cai J, Chen X, Chen B, Liao M, Chai R, and Tang M
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Medicine (General) ,physical cues ,neurites orientation ,R5-920 ,cochlear implants ,migration ,hearing loss - Abstract
Yangnan Hu,1,* Dan Li,2,* Hao Wei,3,* Shan Zhou,1 Wei Chen,1 Xiaoqian Yan,1 Jaiying Cai,1 Xiaoyan Chen,1 Bo Chen,4 Menghui Liao,1 Renjie Chai,1,5 Mingliang Tang1,5,6 1State Key Laboratory of Bioelectronics, School of Life Sciences and Technology, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, 210096, People’s Republic of China; 2School of Biology, Food and Environment, Hefei University, Hefei, 230601, People’s Republic of China; 3Department of Otorhinolaryngology Head and Neck Surgery, Drum Tower Clinical Medical College, Nanjing Medical University, Nanjing, 210000, People’s Republic of China; 4Materials Science and Devices Institute, Suzhou University of Science and Technology, Suzhou, 215009, People’s Republic of China; 5Co-innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, People’s Republic of China; 6Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Medical College, Soochow University, Suzhou, 215000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Renjie Chai; Mingliang Tang Email renjiec@seu.edu.cn; mltang@suda.edu.cnIntroduction: Neuroregeneration is a major challenge in neuroscience for treating degenerative diseases and for repairing injured nerves. Numerous studies have shown the importance of physical stimulation for neuronal growth and development, and here we report an approach for the physical guidance of neuron orientation and neurite growth using superparamagnetic iron oxide (SPIO) nanoparticles and magnetic fields (MFs).Methods: SPIO nanoparticles were synthesized by classic chemical co-precipitation methods and then characterized by transmission electron microscope, dynamic light scattering, and vibrating sample magnetometer. The cytotoxicity of the prepared SPIO nanoparticles and MF was determined using CCK-8 assay and LIVE/DEAD assay. The immunofluorescence images were captured by a laser scanning confocal microscopy. Cell migration was evaluated using the wound healing assay.Results: The prepared SPIO nanoparticles showed a narrow size distribution, low cytotoxicity, and superparamagnetism. SPIO nanoparticles coated with poly-L-lysine could be internalized by spiral ganglion neurons (SGNs) and showed no cytotoxicity at concentrations less than 300 μg/mL. The neurite extension of SGNs was promoted after internalizing SPIO nanoparticles with or without an external MF, and this might be due to the promotion of growth cone development. It was also confirmed that SPIO can regulate cell migration and can direct neurite outgrowth in SGNs preferentially along the direction imposed by an external MF.Conclusion: Our results provide a fundamental understanding of the regulation of cell behaviors under physical cues and suggest alternative treatments for sensorineural hearing loss caused by the degeneration of SGNs.Keywords: physical cues, neurites orientation, hearing loss, cochlear implants, migration
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- 2021
16. Signature Panel of 11 Methylated mRNAs and 3 Methylated lncRNAs for Prediction of Recurrence-Free Survival in Prostate Cancer Patients
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Cai J, Yang F, Chen X, Huang H, and Miao B
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recurrence-free survival ,methylation ,prognostic signature ,Therapeutics. Pharmacology ,RM1-950 ,prostate cancer - Abstract
Jiarong Cai,1,* Fei Yang,1,* Xuelian Chen,1 He Huang,2 Bin Miao3 1Department of Urology, the Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, 510630, People’s Republic of China; 2General Surgery Department, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, 510630, People’s Republic of China; 3Department of Organ Transplantation, the Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, 510630, People’s Republic of China*These authors contributed equally to this workCorrespondence: He HuangGeneral Surgery Department, the Third Affiliated Hospital of Sun Yat-sen University, No. 600, Tianhe Road, Guangzhou, Guangdong, 510630, People’s Republic of ChinaEmail huangh85@mail.sysu.edu.cnBin MiaoDepartment of Organ Transplantation, the Third Affiliated Hospital of Sun Yat-Sen University, No. 600, Tianhe Road, Guangzhou, Guangdong, 510630, People’s Republic of ChinaTel +86-20-82179517Fax +86-20-82179517Email miaobin@mail.sysu.edu.cnBackground: Radical prostatectomy is the main treatment for prostate cancer (PCa), a common cancer type among men. Recurrence frequently occurs in a proportion of patients. Therefore, there is a great need to early screen those patients to specifically schedule adjuvant therapy to improve the recurrence-free survival (RFS) rate. This study aims to develop a biomarker to predict RFS for patients with PCa based on the data of methylation, an important heritable contributor to carcinogenesis.Methods: Methylation expression data of PCa patients were downloaded from The Cancer Genome Atlas (TCGA), Gene Expression Omnibus database (GSE26126), and the European Bioinformatics Institute (E-MTAB-6131). The stable co-methylation modules were identified by weighted gene co-expression network analysis. The genes in modules were overlapped with differentially methylated RNAs (DMRs) screened by MetaDE package in three datasets, which were used to screen the prognostic genes using least absolute shrinkage and selection operator analyses. The prognostic performance of the prognostic signature was assessed by survival curve analysis.Results: Five co-methylation modules were considered preserved in three datasets. A total of 192 genes in these 5 modules were overlapped with 985 DMRs, from which a signature panel of 11 methylated messenger RNAs and 3 methylated long non-coding RNAs was identified. This signature panel could independently predict the 5-year RFS of PCa patients, with an area under the receiver operating characteristic curve (AUC) of 0.969 for the training TCGA dataset and 0.811 for the testing E-MTAB-6131 dataset, both of which were higher than the predictive accuracy of Gleason score (AUC = 0.689). Also, the patients with the same Gleason score (6– 7 or 8– 10) could be further divided into the high-risk group and the low-risk group.Conclusion: These results suggest that our prognostic model may be a promising biomarker for clinical prediction of RFS in PCa patients.Keywords: prostate cancer, recurrence-free survival, methylation, prognostic signature
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- 2021
17. Perioperative Transfusion is Related to the Length of Hospital Stays in Primary Liver Cancer Patients
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Qi Q, Qian X, Zhu X, Cai J, Xia R, and Zhang Q
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primary liver cancer ,length of hospital stays ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,coagulation factor ,bilirubin ,inflammatory factor ,RC254-282 ,transfusion - Abstract
Qi Qi,* Xuemeng Qian,* Xinfang Zhu, Jiajing Cai, Rong Xia, Qi Zhang Department of Transfusion Medicine, Huashan Hospital, Fudan University, Shanghai, 200040, People’s Republic of China*These authors contributed equally to this workCorrespondence: Rong Xia; Qi ZhangDepartment of Transfusion Medicine, Huashan Hospital, Fudan University, 12 Urumqi Middle Road, Shanghai, 200040, People’s Republic of ChinaEmail xiarongcn@126.com; friday0451@163.comPurpose: Blood loss may be corrected with red blood cell transfusion, but may ultimately contribute to negative impacts. This study was a retrospective analysis to assess the impact of perioperative blood transfusion on hospital stay days in liver cancer patients.Methods: We retrospectively examined data from patients with primary liver cancer who underwent curative resection. Patients were divided into perioperative blood transfusion (PBT) and non-PBT groups. Data were given as means and SDs for continuous variables and as counts and percentage for categorical variables. The correlation between blood transfusion and hospital stay days was analyzed by Fisher’s exact test. Multivariable logistic regression analyses were used to identify independent predictors of length of hospital stays.Results: Totally 206/1031 patients (20.3%) were given perioperative transfusion. The mean length of hospital stay was 17.8 days in PBT and 13.9 days in non-PBT groups. Our multivariable logistic regression showed transfusion, total bilirubin, indirect bilirubin, and the ratio of albumin to bilirubin were all indicators of the length of hospital stay days. Perioperative transfusion was also associated with prolonged length of hospital stays (95% CI: 0.395– 0.811, p = 0.002). Transfusion also affected intrinsic coagulation factors (activated partial thromboplastin time, fibrinogen, platelet), inflammatory index (neutrocyte to lymphocyte ratio, monocyte), albumin and bilirubin levels.Conclusion: Perioperative transfusion of blood was associated with a significantly increased length of hospital stays probably via changing intrinsic coagulation and inflammatory factors and bilirubin levels in plasma.Keywords: primary liver cancer, transfusion, length of hospital stays, bilirubin, coagulation factor, inflammatory factor
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- 2021
18. A Novel KIF5B-EGFR Fusion Variant in Non-Small-Cell Lung Cancer and Response to Afatinib: A Case Report
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Wang X, Huang L, Cai J, and Liu A
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afatinib ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,kif5b-egfr ,next-generation sequencing ,egfr fusion ,non-small cell lung cancer ,RC254-282 - Abstract
Xia Wang,1,* Long Huang,1,* Jing Cai,1 Anwen Liu1,2 1Department of Oncology, The Second Affiliated Hospital of Nanchang University, Nanchang, 330000, Jiangxi Province, People’s Republic of China; 2Jiangxi Key Laboratory of Clinical Translational Cancer Research, Nanchang, 330000, Jiangxi Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Anwen LiuDepartment of Oncology, The Second Affiliated Hospital of Nanchang University, No. 1, Minde Street, Nanchang, Jiangxi Province, 330000, People’s Republic of ChinaEmail awliu666@163.comAbstract: Epidermal growth factor receptor (EGFR) fusions are rare genomic events in non-small-cell lung cancer (NSCLC). With advances in detection technology, some uncommon genomic mutation events, including EGFR fusions, have been detected. There are no standard treatment options for NSCLC patients harboring EGFR fusion. Herein, we report a case of KIF5B-EGFR fusion in NSCLC responding to tyrosine kinase inhibitors (TKIs). A 50-year-old male underwent left upper lobectomy followed by adjuvant chemotherapy for pathological stage IA3 lung adenocarcinoma. The tumor tissue was subjected to next-generation sequencing (NGS) and showed a KIF5B-EGFR fusion. When cancer recurrence occurred thirteen months later, the patient received afatinib (40 mg qd) as second-line treatment, and a partial response was observed, which resulted in an 11-month progression-free survival (PFS). This case provides valuable information on the response to afatinib in an NSCLC patient with a novel KIF5B-EGFR fusion. The NGS assay provides a powerful tool for identifying rare or atypical EGFR gene mutations in patients with NSCLC, which should be encouraged in clinical practice.Keywords: KIF5B-EGFR, EGFR fusion, next-generation sequencing, afatinib, non-small cell lung cancer
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- 2021
19. Using citizen science to estimate surficial soil Blue Carbon stocks in Great British saltmarshes
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Craig Smeaton, Annette Burden, Paulina Ruranska, Cai J. T. Ladd, Angus Garbutt, Laurence Jones, Lucy McMahon, Lucy C. Miller, Martin W. Skov, William E. N. Austin, NERC, University of St Andrews. Environmental Change Research Group, University of St Andrews. School of Geography & Sustainable Development, University of St Andrews. Coastal Resources Management Group, University of St Andrews. Marine Alliance for Science & Technology Scotland, University of St Andrews. Scottish Oceans Institute, and University of St Andrews. St Andrews Sustainability Institute
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Global and Planetary Change ,Saltmarsh ,Vegetation ,Wales ,GE ,Great Britain ,DAS ,Ocean Engineering ,Citizen science ,Aquatic Science ,Oceanography ,Carbon ,Ecology and Environment ,Soil ,QE Geology ,Coastal ,Scotland ,England ,Wetland ,Spatial mapping ,QE ,Organic matter ,GE Environmental Sciences ,Water Science and Technology - Abstract
Funding: This research was finically supported by the Natural Environment Research Council funded Carbon Storage in Intertidal Environments (C-SIDE) project (grant NE/R010846/1) with additional support from the Scottish Blue Carbon Forum. A new saltmarsh soil dataset comprising of geochemical and physical property data from 752 soil samples collected through a sampling program supported by citizen scientists has been brought together with existing data to make the first national estimates of the surficial (top 10 cm) soil OC stock for Great British (GB) saltmarshes. To allow the inclusion of secondary data in the soil stock estimate a new bespoke organic matter to organic carbon conversion for GB saltmarsh soil was developed allowing organic matter data measured using loss-on-ignition to be convert to organic carbon content. The total GB surficial soil OC stock is 2.320 ± 0.470 Mt; English saltmarshes hold 1.601 ± 0.426 Mt OC, Scottish saltmarshes hold 0.368 ± 0.091 Mt OC, and Welsh saltmarshes hold 0.351 ± 0.082 Mt OC. The stocks were calculated within a Markov Chain Monte Carlo framework allowing robust uncertainty estimates to be derived for the first time. Spatial mapping tools are available to accompany these stock estimates at individual saltmarsh habitats throughout GB. This data will aid in the protection and management of saltmarshes and represents the first steps towards the inclusion of saltmarsh OC in the national inventory accounting of blue carbon ecosystems. Publisher PDF
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- 2022
20. Class IIa HDAC Downregulation Contributes to Surgery-Induced Cognitive Impairment Through HMGB1-Mediated Inflammatory Response in the Hippocampi of Aged Mice
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Huang C, Cai J, Jin S, Lin Q, Fang Q, Nan K, Han Y, Ge W, Liu Y, Tao Y, Cao H, and Li J
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hmgb1 ,hippocampus ,postoperative cognitive dysfunction ,Pathology ,hdac5 ,RB1-214 ,Therapeutics. Pharmacology ,RM1-950 ,hdac4 ,neuroinflammation - Abstract
Chen-miao Huang,1,2 Jia-jing Cai,1,2 Shao-wu Jin,1,2 Qi-cheng Lin,1,2 Qian-juan Fang,1,2 Ke Nan,1,2 Yuan Han,1,2 Wen-wei Ge,1,2 Yu Liu,1,2 Yuan-xiang Tao,1,3 Hong Cao,1,2 Jun Li1,2 1Department of Anesthesiology and Perioperative Medicine, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, People’s Republic of China; 2Zhejiang Province Key Laboratory of Anesthesiology, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, People’s Republic of China; 3Department of Anesthesiology, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, NJ, 07103, USACorrespondence: Jun Li; Hong CaoDepartment of Anesthesiology and Perioperative Medicine, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, People’s Republic of ChinaTel/Fax +86 577-88002925 Email lijun0068@163.com; caohong1955@21cn.comObjective: Perioperative neurocognitive disorders (PND) are a common complication in the elderly. Histone deacetylases (HDACs) are a class of enzymes that control the acetylation status of intracellular proteins. Thus, we explored whether HDACs trigger the release of high mobility group box 1 (HMGB1)through altering the acetylation status in the hippocampi of aged mice.Materials and Methods: The effect of the Class IIa HDAC in PND was explored using an in vivo form of splenectomy. Sixteen-month-old healthy male C57BL/6J mice were randomly divided into five groups: control, anesthesia plus sham surgery, anesthesia plus splenectomy, LMK235 treatment, and PBS treatment. The hippocampi were harvested on either first, third, or seventh postoperative day. Cognitive function was assessed via a Morris water maze (MWM) test. Quantitative RT-PCR, Western blots and ELISAs were carried out to assess the targeted gene expression at transcriptional and translational levels.Results: Splenectomy led to a significant deficiency in spatial memory acquisition, marked decreases in mRNA and protein levels of HDAC4 and HDAC5 in the hippocampus, and increases in the levels of total HMGB1 and acetylated HMGB1. In a similar fashion to splenectomy, treatment with the HDAC4/5 inhibitor LMK235 produced impaired spatial memory and an increase in the expression of HMGB1 and its acetylated counterpart in the hippocampus.Conclusion: These results suggest that surgery leads to PND through class IIa HDAC downregulation-triggered HMGB1 release in hippocampus of aged mice. HDACs may be a potential therapeutic target for postoperative cognitive dysfunction.Keywords: postoperative cognitive dysfunction, hippocampus, HDAC4, HDAC5, HMGB1, neuroinflammation
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- 2021
21. Inhibition of circRNA circVPS33B Reduces Warburg Effect and Tumor Growth Through Regulating the miR-873-5p/HNRNPK Axis in Infiltrative Gastric Cancer
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Lu Y, Cheng J, Cai W, Zhuo H, Wu G, and Cai J
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circvps33b ,hnrnpk ,infiltrative gc ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,mir-873-5p ,RC254-282 - Abstract
Yizhuo Lu,1 Jia Cheng,2 Wangyu Cai,2 Huiqin Zhuo,2 Guoyang Wu,1 Jianchun Cai2 1Department of General Surgery, Zhongshan Hospital Xiamen University, Institute of Gastrointestinal Oncology, School of Medicine, Xiamen University, Xiamen Municipal Key Laboratory of Gastrointestinal Oncology, Xiamen, Fujian, 361004, People’s Republic of China; 2Department of Gastrointestinal Surgery, Zhongshan Hospital Xiamen University, Institute of Gastrointestinal Oncology, School of Medicine, Xiamen University, Xiamen Municipal Key Laboratory of Gastrointestinal Oncology, Xiamen, Fujian, 361004, People’s Republic of ChinaCorrespondence: Jianchun CaiDepartment of Gastrointestinal Surgery, Zhongshan Hospital Xiamen University, Institute of Gastrointestinal Oncology, School of Medicine, Xiamen University, Xiamen Municipal Key Laboratory of Gastrointestinal Oncology, Room 203, No. 146, Hubin South Road, Siming District, Xiamen, Fujian Province, 361004, People’s Republic of ChinaTel +86 592-2993191Email jianchuncai0592@163.comBackground: Circular RNA VPS33B (circVPS33B) has been revealed to be upregulated in gastric cancer (GC) tissues. However, the role of circVPS33B in infiltrative GC is indistinct.Methods: Expression of circVPS33B was detected using quantitative real-time polymerase chain reaction (qRT-PCR). The proliferation, migration, and invasion of infiltrative GC cells (XGC-1) were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT), plate clone, wound-healing, or transwell assays. Protein levels were detected by Western blotting. Measurements of extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) were executed using an XF96 extracellular flux analyzer. Glucose uptake and lactate production were analyzed by glycolysis assay. The regulatory mechanism of circVPS33B had been explored by bioinformatics analysis, dual-luciferase reporter assay, and/or RNA pull-down assay. In vivo tumorigenesis assay was executed to verify the oncogenicity of circVPS33B.Results: CircVPS33B was upregulated in infiltrative GC tissues and cells. CircVPS33B silencing decreased tumor growth in vivo and inhibited proliferation, migration, invasion, EMT, and Warburg effect of infiltrative GC cells in vitro. Mechanically, circVPS33B regulated heterogeneous nuclear ribonucleoprotein K (HNRNPK) expression via sponging miR-873-5p. Furthermore, miR-873-5p inhibitor offset circVPS33B knockdown-mediated effects on malignant behaviors and Warburg effect of infiltrative GC cells. HNRNPK overexpression reversed the inhibitory impact of miR-873-5p mimic on malignant behaviors and Warburg effect of infiltrative GC cells.Conclusion: CircVPS33B accelerated Warburg effect and tumor growth through regulating the miR-873-5p/HNRNPK axis in infiltrative GC, manifesting that circVPS33B might be a potential target for infiltrative GC treatment.Keywords: circVPS33B, miR-873-5p, HNRNPK, infiltrative GC
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- 2021
22. Investigation and Analysis of the Colonization and Prevalence of Carbapenem-Resistant Enterobacteriaceae in Pediatric Liver Transplant Recipients
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Sun Y, Yu L, Gao W, Cai J, Jiang W, Lu W, Liu Y, and Zheng H
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liver transplantation ,ct screening ,intestinal colonization ,Infectious and parasitic diseases ,RC109-216 ,carbapenem-resistant enterobacteriaceae (cre) ,anal swabs - Abstract
Yan Sun,1,2 Lixin Yu,1,2 Wei Gao,1,2 Jinzhen Cai,1,2 Wentao Jiang,1,2 Wei Lu,3 Yihe Liu,1,2 Hong Zheng1,2 1Organ Transplantation Center, Tianjin First Central Hospital, Tianjin, 300192, People’s Republic of China; 2Tianjin Key Laboratory for Organ Transplantation, Tianjin, People’s Republic of China; 3Liver Cancer Treatment Centre, Tianjin Medical University Cancer Institute and Hospital, Tianjin, People’s Republic of ChinaCorrespondence: Yihe Liu; Hong ZhengOrgan Transplantation Center, Tianjin First Central Hospital, No. 24 of Fukang Street, Nankai District, Tianjin, 300192, People’s Republic of ChinaTel/Fax +8602223627027Email limyihe@163.com; zhemghomgi@21cn.comObjective: This study aimed to investigate the colonization and prevalence of carbapenem-resistant Enterobacteriaceae (CRE) in pediatric liver transplant recipients and analyze the high-risk factors and prognosis of CRE infection.Methods: A prospective study involving 152 pediatric patients undergoing liver transplantation was carried out. Anal swab bacteria cultures were collected when the patients entered the intensive care unit (ICU) and when they left in order to screen for intestinal CRE colonization. The results were grouped according to the occurrence of CRE infection following surgery, and the patients were divided into two groups: a CRE infection group and a non-CRE infection group. Univariate analysis and multiple logistic regression analysis were conducted to determine the independent risk factors of CRE infection and analyze the survival rate.Results: Of the 152 pediatric liver transplant recipients enrolled in the study, there were 13 cases of postoperative CRE infection and 139 cases of non-CRE infection. The incidence of preoperative CRE infection, preoperative cytomegalovirus (CMV) infection, and preoperative sepsis in the CRE infection group was significantly higher than in the non-CRE infection group (P < 0.005). Intraoperative bleeding volume and operation times in the CRE infection group were also significantly higher than in the non-CRE infection group (P < 0.05). Furthermore, postoperative ICU treatment time, postoperative occurrence of unplanned surgery, postoperative mechanical ventilation of more than 24 hours, and the incidence of pre-ICU CRE colonization in the CRE infection group were significantly higher than in the non-CRE infection group (P < 0.05). Finally, the difference between the CRE infection group and the non-CRE infection group in six-month survival rate following surgery was significant (P < 0.001).Conclusion: The independent risk factors of CRE infection following pediatric liver transplantation include preoperative CRE infection and pre-ICU CRE colonization. CRE infection progresses quickly, with a poor prognosis and a high mortality rate. The CRE screening of anal swabs is crucial for the early detection of CRE infection.Keywords: carbapenem-resistant Enterobacteriaceae, CRE, liver transplantation, intestinal colonization, CT screening, anal swabs
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- 2021
23. EGFR (p. G719A+L747V)/EML4-ALK Co-alterations in Lung Adenocarcinoma with Leptomeningeal Metastasis Responding to Afatinib Treatment: A Case Report
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Lu Z, Wang X, Luo Y, Wei J, Zeng Z, Xiong Q, Cai J, and Liu A
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co-alterations ,alk ,leptomeningeal metastasis ,egfr ,afatinib ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,lung adenocarcinoma ,RC254-282 - Abstract
Zhiqin Lu,1 Xia Wang,1,2 Yuxi Luo,1 Jianping Wei,1 Zhimin Zeng,1,2 Qiang Xiong,1,2 Jing Cai,1,2 Anwen Liu1,2 1Department of Oncology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, People’s Republic of China; 2Jiangxi Key Laboratory of Clinical Translational Cancer Research, Nanchang, Jiangxi Province, People’s Republic of ChinaCorrespondence: Jing Cai; Anwen LiuDepartment of Oncology, The Second Affiliated Hospital of Nanchang University, No. 1, Minde Street, Nanchang, Jiangxi Province, 360000, People’s Republic of ChinaTel +8615270905381; Tel +8615270905381Email cjdl879@163.com; awliu666@163.comAbstract: Leptomeningeal metastasis (LM) is a disastrous complication of advanced lung adenocarcinoma (LAC) associated with poor prognosis and rapid deterioration of performance status. The prevalence of epidermal growth factor receptor (EGFR)/anaplastic lymphoma kinase (ALK) co-alterations in patients with LAC was low. Herein, we report a patient with alterations in both EGFR (p. G719A+L747V) and echinoderm microtubule-associated protein-like ALK (EML4-ALK) fusion and LM who was treated with afatinib. The patient’s clinical symptoms improved, and imaging examination revealed reduced intracranial and extracranial lesions. The progression-free survival (PFS) using afatinib for LM was 25 months, and no severe adverse events occurred.Keywords: afatinib, leptomeningeal metastasis, EGFR, ALK, co-alterations, lung adenocarcinoma
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- 2021
24. Primary Exophytic Extraskeletal Osteosarcoma of the Liver: A Case Report and Literature Review
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Zhang J, He X, Yu W, Ying F, Cai J, and Deng S
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extraskeletal ,osteosarcoma ,lcsh:Public aspects of medicine ,primary ,tumor bone ,lcsh:RA1-1270 ,hepatic - Abstract
Jing Zhang,1 Xiuchao He,1 Wenying Yu,2 Fuming Ying,3 Jun Cai,4 Shengde Deng1 1Department of Imaging Center, The Affiliated Hospital of Medical School of Ningbo University, Ningbo, Zhejiang Province, 315000, People’s Republic of China; 2Ningbo Diagnostic Pathology Center, Ningbo, Zhejiang Province, 315000, People’s Republic of China; 3Department of Hepatobiliary Surgery, The Affiliated Hospital of Medical School of Ningbo University, Ningbo, Zhejiang Province, 315000, People’s Republic of China; 4Department of PET-CT Diagnosis Center, Mingzhou Hospital of Zhejiang University, Ningbo, Zhejiang Province, 315000, People’s Republic of ChinaCorrespondence: Shengde DengDepartment of Imaging Center, The Affiliated Hospital of Medical School of Ningbo University, No. 247 of Renmin Street, Jiangbei District, Ningbo, Zhejiang Province, 315000, People’s Republic of ChinaTel/Fax +86 574 55873876Email demgshemgde@163.comIntroduction: Primary hepatic extraskeletal osteosarcoma (ESOS) is a rare tumor with no specific clinical manifestations, and little is known about it. Here, we describe an elderly patient with primary hepatic osteosarcoma confirmed by pathology results to raise awareness.Case Report: We report an unusual case of a 62-year-old man who presented with right upper quadrant pain. The inflammatory indicators were elevated, and alkaline phosphatase (AKP), carbohydrate antigen (CA-199 and CA-125) were slightly increased. Computed tomography images and magnetic resonance images discovered a 7.8 × 7.4 × 6.6 cm mass with irregular radiated and cotton-like tumor bone between the liver and right kidney space. Pathology revealed the mass to be primary exophytic ESOS of the liver. The patient underwent a surgical operation and standard chemotherapy and is still alive with no recurrence and metastasis to date.Conclusion: Owing to the rarity of the tumor and the lack of clinical characteristics and specific laboratory indexes, it is difficult to make a correct diagnosis. Medical imaging features mainly behave soft tissue entity with tumor bone composition. Surgical resection combined with adjuvant chemotherapy is the main treatment for ESOS.Keywords: extraskeletal, osteosarcoma, hepatic, tumor bone, primary
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- 2021
25. Silencing of PTPN18 Induced Ferroptosis in Endometrial Cancer Cells Through p-P38-Mediated GPX4/xCT Down-Regulation
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Wang H, Peng S, Cai J, and Bao S
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p-p38 ,gpx4/xct ,parasitic diseases ,endometrial cancer ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,lcsh:RC254-282 ,ferroptosis ,ptpn18 - Abstract
Haibo Wang,1,* Siyuan Peng,1,* Junhong Cai,2 Shan Bao1 1Department of Gynaecology and Obstetrics, Hainan General Hospital/Hainan Affiliated Hospital of Hainan Medical University, Haikou, 570311, People’s Republic of China; 2Key Laboratory of Cell and Molecular Genetic Translational Medicine in Hainan Province, Hainan General Hospital/Hainan Affiliated Hospital of Hainan Medical University, Haikou, 570311, People’s Republic of China*These authors contributed equally to this workCorrespondence: Shan BaoDepartment of Gynaecology and Obstetrics, Hainan General Hospital/Hainan Affiliated Hospital of Hainan Medical University, Haikou, 570311, People’s Republic of ChinaEmail baoshan3@hainmc.edu.cnBackground: Endometrial cancer (EC) is the fourth most common neoplasm and the eighth leading cause of cancer death in females worldwide. PTPN18 is a member of the protein tyrosine phosphatases (PTP) family, which is associated with the occurrence and progression of various human cancers. PTPN18 was up-regulated in endometrial cancer tissues and high level of PTPN18 promoted proliferation and metastasis of EC cells.Methods: The expression of PTPN18, GPX4 and xCT in endometrial cancer tissues and KLE cells was detected by immunohistochemistry and Western blot, respectively. Lentiviral transfection were used to silence PTPN18 level in KLE cells. The Ros level in KLE cells was examined by ELISA assay.Results: In the present study, we found that silencing of PTPN18 induced ferroptosis in KLE endometrial cancer cells. PTPN18 knockdown increased intracellular ROS level and down-regulated GPX4 and xCT expression. Besides, silencing of PTPN18 also induced the expression of p-p38.Conclusion: We concluded that silencing of PTPN18 might induce ferroptosis by targeting the p-p38/GPX4/xCT axis. The results provide critical insight into the application of PTPN18 knockdown in EC intervention.Keywords: PTPN18, endometrial cancer, ferroptosis, p-p38, GPX4/xCT
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- 2021
26. TEAD4 as a Prognostic Marker Promotes Cell Migration and Invasion of Urinary Bladder Cancer via EMT
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Huang Z, Yan Y, Tang P, Cai J, Cao X, Wang Z, Zhang F, and Shen B
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urinary bladder cancer ,emt ,tead4 ,prognostic biomarker ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,lcsh:RC254-282 ,migration and invasion - Abstract
Zhengnan Huang,1,* Yilin Yan,1,* Pengfei Tang,2 Jinming Cai,1 Xiangqian Cao,1 Zeyi Wang,2 Fang Zhang,1 Bing Shen1,2 1Department of Urology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200080, People’s Republic of China; 2Department of Urology, Shanghai General Hospital Affiliated to Nanjing Medical University, Shanghai, 200080, People’s Republic of China*These authors contributed equally to this workCorrespondence: Bing Shen; Fang ZhangDepartment of Urology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, No. 100 Haining Road, Hongkou District, Shanghai, 200080, People’s Republic of ChinaTel +86-021-5213-0011-361; +86-21-63240090-2514Email shenbingyishi@163.com; medego@163.comPurpose: As a member of TEA Domain Transcription Factors (TEADs), TEAD4 was found to be upregulated in urinary bladder cancer (UBC). This study focused on investigating the clinical value and potential functions of TEAD4 in UBC.Materials and Methods: Patients’ samples, TCGA-BLCA and multiple GEO datasets were applied to explore the expression pattern of TEAD4 in UBC. Cox regression and Kaplan–Meier survival analyses were carried out to evaluate the prognostic significance of TEAD4 in UBC. Wound healing and transwell assays were performed to explore the biological functions of TEAD4 in UBC cells.Results: The results of TCGA-BLCA, GEO datasets, Western blotting and immunohistochemistry staining (IHC) indicated that TEAD4 was strikingly elevated in UBC tissues as compared to their normal counterparts, and upregulation of TEAD4 was significantly correlated with clinical stage, pathological grade and poor clinical outcome. Functional studies demonstrated that TEAD4 knockdown suppressed cell migration and invasion by reducing the expression of epithelial-mesenchymal transition (EMT) related markers and transcription regulators.Conclusion: Our results suggest that TEAD4 may serve as a novel prognostic biomarker and a promising therapeutic target for UBC, and act as a pro-tumorigenic gene to promote cell migration and invasion by inducing EMT.Keywords: TEAD4, urinary bladder cancer, prognostic biomarker, migration and invasion, EMT
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- 2021
27. GADD45B Facilitates Metastasis of Ovarian Cancer Through Epithelial–Mesenchymal Transition
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Gong L, Cai L, Li G, Cai J, and Yi X
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epithelial ovarian cancer ,gadd45b ,epithelial-mesenchymal transition ,metastasis ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,lcsh:RC254-282 - Abstract
Lanqing Gong,1,* Liqiong Cai,1,* Guodong Li,2 Jing Cai,1 Xiaoqing Yi1 1Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People’s Republic of China; 2Cancer Research Institute, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430022, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xiaoqing YiDepartment of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan, Hubei Province 430022, People’s Republic of ChinaTel/Fax: +86 27 85351649Email yxq1987724@126.comBackground: Growth arrest and DNA-damage-inducible 45 beta (GADD45B) is overexpressed and is associated with poor clinical outcomes in many human cancers, but the clinical implication of GADD45B in epithelial ovarian cancer (EOC) remains unclear.Methods: Bioinformatics analysis of The Cancer Genome Atlas (TCGA) and gene expression omnibus (GEO) cohorts was used to illustrate the relationship between GADD45B expression and metastasis, as well as the survival time of EOC. GADD45B was downregulated by siRNAs in EOC cells, and migration ability was determined by a transwell assay and wound-healing assay. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis and gene set enrichment analysis (GSEA) were conducted to discover the downstream pathway of GADD45B. The regulation of epithelial–mesenchymal transition (EMT) by GADD45B was verified by Western blotting and qRT-PCR. Finally, the correlation of GADD45B expression with EOC metastasis was investigated in EOC tissues by immunohistochemistry.Results: Overexpression of GADD45B indicates shorter overall survival time and progression-free survival time, and it is an independent risk factor for poor survival in EOC patients. Elevated GADD45B is related to venous invasion, lymphatic invasion and peritoneal carcinomatosis. Downregulation of GADD45B decreases the migration of ES2 and SKOV3 cells. Further KEGG enrichment analysis and GSEA revealed that EMT may be the downstream pathway of GADD45B. In addition, reduced GADD45B increases the expression of E-cadherin and decreases that of N-cadherin and vimentin. Finally, immunohistochemical analysis of GADD45B expression revealed that the expression of GADD45B in omental metastatic tissues was higher than that in matched primary ovarian cancer tissues. These results suggest that elevated GADD45B promotes the motility of ovarian cancer cells through EMT and is associated with EOC metastasis.Conclusion: GADD45B can promote the motility of ovarian cancer cells through EMT, is associated with EOC metastasis, and may be a new biomarker of metastasis and prognosis.Keywords: epithelial ovarian cancer, GADD45B, metastasis, epithelial–mesenchymal transition
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- 2021
28. miR-124-3p Regulates FGF2–EGFR Pathway to Overcome Pemetrexed Resistance in Lung Adenocarcinoma Cells by Targeting MGAT5
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Cai J, Huang J, Wang W, Zeng J, and Wang P
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mir-124-3p ,mgat5 ,fgf2-egfr pathway ,lung adenocarcinoma ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,lcsh:RC254-282 - Abstract
Jundong Cai,1 Jiuning Huang,1,2 Wulong Wang,1 Jing Zeng,1 Ping Wang1 1Department of Radiotherapy, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin’s Clinical Research Center for Cancer, Tianjin 300000, People’s Republic of China; 2Department of Radiotherapy, Yantai Affiliated Hospital of Binzhou Medical University, Yantai 264000, Shandong, People’s Republic of ChinaCorrespondence: Ping WangDepartment of Radiotherapy, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin’s Clinical Research Center for Cancer, Huanhu Xi Road, Tiyuan Bei, Hexi District, Tianjin 300000, People’s Republic of ChinaTel +86-18622221112Email pingwang2020@163.comObjective: To investigate whether miR-124-3p regulates the fibroblast growth factor 2 (FGF2)–epidermal growth factor receptor (EGFR) pathway by targeting MGAT5 to affect the pemetrexed resistance in lung adenocarcinoma cells.Methods: PC9-MTA and H1993-MTA anti-pemetrexed lung adenocarcinoma cell lines were constructed. The cell viability of anti-pemetrexed and parent lung adenocarcinoma cells was analyzed using MTS assay and reverse transcription PCR to determine the expression of miR-124-3p. CCK8 assay, colony formation assay, and flow cytometry were used to determine cells’ proliferation and apoptosis. FGF2–EGFR signaling pathway-related proteins and MGAT5 protein expression were quantified by Western blotting. The target relationship between miR-124-3p and MGAT5 was verified by double luciferase assay. A nude mouse model with a transplanted tumor was established using the anti-pemetrexed lung adenocarcinoma cells. Tumor volume and weight were determined, and the apoptosis of tumor cells was observed.Results: The half-maximal inhibitory concentration of pemetrexed in anti-pemetrexed lung adenocarcinoma cells was higher than that in parent lung adenocarcinoma cells, and the expression of miR-124-3p in the anti-pemetrexed cells was lower than that of the parent cells. In the miR-124-3p overexpression group, MGAT5 silencing group, and miR-124-3p+MGAT5 overexpression group, compared with the control group, the proliferation ability of cells and tumors was markedly reduced; their apoptosis rates were increased significantly; expression levels of FGF2 and p-EGFR/EGFR were decreased; and the growth rate and tumor volume and mass were reduced; however, the opposite results were obtained in the miR-124-3p silencing group (p< 0.05).Conclusion: miR-124-3p may inhibit the FGF2–EGFR pathway by targeting MGAT5 to decrease pemetrexed resistance in lung adenocarcinoma cells.Keywords: miR-124-3p, MGAT5, FGF2-EGFR pathway, lung adenocarcinoma
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- 2020
29. The Efficacy and Response Predictors of Platinum-Based Neoadjuvant Chemotherapy in Locally Advanced Cervical Cancer
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Huang Y, Liu L, Cai J, Yang L, Sun S, Zhao J, Xiong Z, and Wang Z
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locally advanced cervical cancer ,predictor ,prognosis ,clinical response ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,lcsh:RC254-282 ,neoadjuvant chemotherapy - Abstract
Yuhui Huang,* Lin Liu,* Jing Cai, Lu Yang, Si Sun, Jing Zhao, Zhoufang Xiong, Zehua Wang Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zehua Wang; Zhoufang XiongDepartment of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan 430022, Hubei, People’s Republic of ChinaTel/Fax +86 27 85351649Email zehuawang@163.net; xiaoxiongmaomi@aliyun.comObjective: To assess the efficacy of platinum-based neoadjuvant chemotherapy (NACT) in patients with locally advanced cervical cancer (LACC) and investigate the pretreatment predictors of the response.Patients and Methods: A total of 219 patients with International Federation of Gynecology and Obstetrics (FIGO 2009) stage IB2-IIA2 LACC who received platinum-based NACT from December 2007 to December 2017 were reviewed, and their clinical-pathological characteristics and follow-up data were retrospectively collected and analyzed. The baseline characteristics of age, FIGO stage, histology, tumor differentiation, tumor size, and clinical outcomes, including post-operative pathological risk factors, overall survival (OS), and progression-free survival (PFS) were compared between the responders and non-responders.Results: The overall response rate was 58.9% (129/219), and 19 (8.7%) patients achieved pathologically complete remission. NACT responders showed significantly better OS and PFS than non-responders (POS= 0.002, PPFS= 0.002). The response to NACT was identified as an independent risk factor for OS (hazard ratio [HR] = 2.453, 95% confidence intervals [95% CI], 1.125– 5.348, P = 0.024) and PFS (HR = 2.196, 95% CI, 1.183– 4.076, P = 0.013), and patients with IB2/IIA1 and a tumor size of < 5 cm tended to receive better response than patients with IIA2 (P = 0.004) and a tumor size of ≥ 5 cm (P = 0.027).Conclusion: The response rate of platinum-based NACT was approximately 60%. The response to NACT was an independent risk factor for prognosis, and patients with earlier stage and smaller tumor tended to respond better to NACT.Keywords: locally advanced cervical cancer, neoadjuvant chemotherapy, clinical response, predictor, prognosis
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- 2020
30. Downregulation of circNRIP1 Suppresses the Paclitaxel Resistance of Ovarian Cancer via Regulating the miR-211-5p/HOXC8 Axis
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Li M, Cai J, Han X, and Ren Y
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ptx resistance ,hoxc8 ,oc ,circnrip1 ,mir-211-5p ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,lcsh:RC254-282 - Abstract
Meng Li,1 Junna Cai,1 Xiaorui Han,2 Yue Ren1 1Department of Gynaecology, Xingtai People’s Hospital, Xingtai, Hebei 054000, People’s Republic of China; 2Department of Obstetrics and Gynecology, Xingtai People’s Hospital, Xingtai, Hebei 054000, People’s Republic of ChinaCorrespondence: Yue RenDepartment of Gynaecology, Xingtai People’s Hospital, Xingtai, Hebei 054000, People’s Republic of ChinaEmail hkmx7bb@163.comBackground: Circular RNA (circRNA) has an essential regulatory role in the chemotherapy resistance of cancers. Nevertheless, the role of circRNA nuclear receptor-interacting protein 1 (circNRIP1) in the paclitaxel (PTX) resistance of ovarian cancer (OC) remains unclear.Material and Methods: The circNRIP1, miR-211-5p and homeobox C8 (HOXC8) expression levels were assessed using qRT-PCR. The PTX resistance of cells was measured by 3-(4, 5-dimethylthiazolyl-2-yl)-2-5 diphenyl tetrazolium bromide (MTT) assay. Furthermore, cell proliferation, apoptosis, migration and invasion were detected by colony formation assay, flow cytometry and transwell assay, respectively. Moreover, the protein levels of proliferation, apoptosis, metastasis-related markers and HOXC8 were determined by Western blot (WB) analysis. Tumor xenograft models were constructed to explore the influence of circNRIP1 on OC tumor growth. The interaction between miR-211-5p and circNRIP1 or HOXC8 was confirmed by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay.Results: CircNRIP1 was highly expressed in PTX-resistant OC tissues and cells. Silencing of circNRIP1 repressed the PTX resistance of OC cells in vitro and OC tumor in vivo. Furthermore, circNRIP1 sponged miR-211-5p, and miR-211-5p inhibitor could reverse the inhibitory effect of circNRIP1 knockdown on the PTX resistance of OC cells. In addition, miR-211-5p targeted HOXC8, and HOXC8 overexpression could reverse the suppression effect of miR-211-5p on the PTX resistance of OC cells. Additionally, the expression of HOXC8 was regulated by circNRIP1 and miR-211-5p.Conclusion: CircNRIP1 silencing could inhibit the PTX resistance of OC via regulating the miR-211-5p/HOXC8 axis, showing that circNRIP1 might be a potential target for OC resistance treatment.Keywords: ovarian cancer, PTX resistance, circNRIP1, miR-211-5p, HOXC8
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- 2020
31. Circulating Tie2-Expressing Monocytes: A Potential Biomarker for Cervical Cancer
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Han Q, Zhang Q, Ying F, Wang Z, Zhang Y, Gong L, Cai E, Qian J, and Cai J
- Subjects
lymph node metastasis ,cervical cancer ,tumor associated macrophages ,tie2-expressing monocytes ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,lcsh:RC254-282 - Abstract
Qing Han,* Quan Zhang,* Feiquan Ying, Zehua Wang, Yifan Zhang, Lanqing Gong, E Cai, Jiaxian Qian, Jing Cai Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430022, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jing Cai; Jiaxian Qian Department of Obstetrics and Gynecology, Union HospitalTongji Medical College, Huazhong University of Science and Technology, No. 1277 Jiefang Avenue, Wuhan, Hubei 430022, People’s Republic of ChinaTel +86 135 5451 1709; +86 157 1715 6977Fax +86 27 8572 6993Email caijingmmm@hotmail.com; qianjiaxian@hust.edu.cnBackground: Tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 2 (Tie2)-expressing monocytes (TEMs) are a highly proangiogenic subset of myeloid cells, which are characterized by expressing the angiopoietin receptor Tie2 with pro-tumor activity.Purpose: The present study aimed to determine the clinical value of circulating TEMs (cTEMs) for cervical cancer.Patients and Methods: Peripheral blood mononuclear cells (PBMCs) were obtained from 7 healthy volunteers, 17 uterine fibroid patients, 24 cervical intraepithelial neoplasia (CIN) II patients, 31 CIN III patients and 99 patients with cervical cancer. The cTEMs were evaluated by the ratio of Tie2+ CD14+ cells to all CD14+ monocytes in the PBMCs through flow cytometry. The diagnostic value of cTEM was assessed by receiver operating characteristic (ROC) curves and the correlation between cTEM and clinicopathological characters in cervical cancer patients was analyzed.Results: The proportion of cTEMs was gradually increasing from healthy volunteers to patients with non-invasive lesions, then to cervical cancer patients. The area under the ROC curve was 0.913 when the level of cTEMs was used to distinguish cervical cancer from all the other women ranging from healthy volunteers to CIN III patients. In cervical cancer, an increased cTEM fraction was significantly correlated with advanced tumor stage, larger tumor size, lymph node metastasis (LNM), deep stromal infiltration, parametrial involvement and lymph-vascular space invasion and was an independent risk factor for LNM.Conclusion: The cTEM proportion might be a promising biomarker for the malignant transformation of cervical lesions and the progression of cervical cancer.Keywords: cervical cancer, Tie2-expressing monocytes, lymph node metastasis, tumor associated macrophages, biomarker, tumor progression
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- 2020
32. Dual-Effect of Magnetic Resonance Imaging Reporter Gene in Diagnosis and Treatment of Hepatocellular Carcinoma
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Zhou J, Zhou Q, Shu G, Wang X, Lu Y, Chen H, Hu T, Cai J, Du Y, and Yu R
- Subjects
Medicine (General) ,R5-920 ,embryonic structures ,afp promoter ,magnetic resonance imaging ,transferrin ,hepatocellular carcinoma ,targeted drug delivery - Abstract
Jiaping Zhou,1 Qiaomei Zhou,1 Gaofeng Shu,2 Xiaojie Wang,1 Yuanfei Lu,1 Haiyan Chen,1 Tingting Hu,1 Jinsong Cai,1 Yongzhong Du,2 Risheng Yu1 1Department of Radiology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, People’s Republic of China; 2Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, People’s Republic of ChinaCorrespondence: Yongzhong DuInstitute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, Zhejiang Province, People’s Republic of ChinaTel +86 571 8820 8435Email duyongzhong@zju.edu.cnRisheng YuDepartment of Radiology, Second Affiliated Hospital, School of Medicine, Zhejiang University, 88 Jiefang Road, Hangzhou 310009, Zhejiang Province, People’s Republic of ChinaTel +86 571 8778 3925Email risheng-yu@zju.edu.cnPropose: The early diagnosis of hepatocellular carcinoma (HCC) with ferritin heavy chain (Fth) modified by alpha-fetoprotein (AFP) promoter has been studied. However, no study has focused on the considerable upregulation and specific targeting effects of transferrin receptors (TfR) caused by the transfection of plasmids encoded with the AFP promoter. Thus, the objective of our study was to investigate whether the transfection of Fth gene modified with AFP promoter (AFP@Fth) could be used for early diagnosis and enhanced treatment of HCC.Methods: The AFP@Fth plasmid was transfected into AFP positive cells. The expression of intracellular Ferritin was verified by Western blot, and the upregulation of TfR was confirmed by immunofluorescence and flow cytometry analysis. Cellular iron accumulation resulting in decreased imaging signals was examined by magnetic resonance imagining. Doxorubicin liposome modified with transferrin (Tf-LPD) was prepared to investigate the efficiency of the subsequent treatment after transfection. The enhanced drug distribution and effects were investigated both in vitro and in vivo.Results: Both Ferritin and TfR were overexpressed after transfection. The transfected cells showed higher intracellular iron accumulation and resulted in a lower MR T2-weighted imaging (T2WI) intensity, suggesting that the transfection of AFP@Fth could be a potential strategy for early diagnosis of liver cancer. The following treatment efficacy was revealed by Tf-LPD. As compared with un-transfected cells, transfected cells exhibited higher uptake of transferrin-modified liposomes (Tf-LP), which was due to the specific interaction between Tf and TfR overexpressed on the transfected cells. This is also the reason why Tf-LPD showed better in vitro and in vivo anticancer ability than doxorubicin loaded liposome (LPD). These results suggested that transfection of AFP@Fth could result in enhanced therapy of liver cancer.Conclusion: Transfection of AFP@Fth could be used for early diagnosis and for enhanced treatment of live cancers.Keywords: AFP promoter, magnetic resonance imaging, transferrin, targeted drug delivery, hepatocellular carcinoma
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- 2020
33. Combination of Immune Checkpoint Inhibitors with Chemotherapy in Lung Cancer
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Liu W, Zhang L, Xiu Z, Guo J, Wang L, Zhou Y, Jiao Y, Sun M, and Cai J
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lung cancer ,icis therapy ,chemotherapy ,immunomodulation ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,lcsh:RC254-282 - Abstract
Wei Liu,1,2 Lei Zhang,1,2 Zhiming Xiu,2 Jian Guo,1 Liye Wang,3 Yue Zhou,4 Yang Jiao,1 Meiyan Sun,1 Jianhui Cai1,2 1College of Laboratory Medicine, Jilin Medical University, Jilin 132013, People’s Republic of China; 2Jilin Collaborative Innovation Center for Antibody Engineering, Jilin Medical University, Jilin 132013, People’s Republic of China; 3Department of Pharmacological and Pharmaceutical Science, College of Pharmacy, University of Houston, Houston, TX 77030, USA; 4Department of Statistics, North Dakota State University, Fargo, ND 58102, USACorrespondence: Jianhui Cai; Meiyan Sun Email caijianhui19640917@163.com; sunmy990@163.comAbstract: Tremendous progress has been achieved in the field of immune checkpoint inhibitors (ICIs) therapy in lung cancer in recent years. To generate robust, long-lasting anti-tumor immune responses in lung cancer patients, combinational ICI therapies have been explored deeply. Conventionally, chemotherapy was considered as immunosuppressive. It is now recognized that chemotherapy could also reinstate cancer cell immune-surveillance and enable the perception of cancer cells as dangerous. That is to say that chemotherapeutic drugs are not only a source of direct cytotoxic effects but also an adjuvant for anti-tumor immunity. Recently, multiple clinical studies of ICIs combined with chemotherapeutic drugs have been explored and proved effective. However, there are still crucial questions that are not well addressed, such as the optimal dose and schedule for a given combination may differ across disease indications, and the appropriate strategy of selecting patient population that can benefit from ICIs remains unclear. To facilitate more rational lung cancer ICIs therapy development, this review summarizes the immune-regulatory effects and related mechanisms of chemotherapeutic drugs and the clinical progress of ICIs and their combination with chemotherapies in lung cancer treatment.Keywords: ICIs therapy, chemotherapy, lung cancer, immunomodulation
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- 2020
34. Minicircle DNA-Mediated CAR T Cells Targeting CD44 Suppressed Hepatocellular Carcinoma Both in vitro and in vivo
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Wang H, Ye X, Ju Y, Cai Z, Wang X, Du P, Zhang M, Li Y, and Cai J
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cancer immunotherapy ,chimeric antigen receptor ,cancer stem cell antigen ,tumor suppression ,nonviral vector ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,lcsh:RC254-282 - Abstract
Hezhi Wang,1 Xueshuai Ye,1,2 Yi Ju,3 Ziqi Cai,2 Xiaoxiao Wang,4 Pingping Du,2 Mengya Zhang,2 Yang Li,1 Jianhui Cai1,5 1Department of Surgery, Hebei Medical University, Shijiazhuang, Hebei, People’s Republic of China; 2Hebei Engineering Technology Research Center for Cell Therapy, Hebei HOFOY Biotech Corporation Ltd., Shijiazhuang, Hebei, People’s Republic of China; 3Department of Medicine, Medical College of Hebei University of Engineering, Handan, Hebei, People’s Republic of China; 4Department of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei, People’s Republic of China; 5Department of Surgery & Oncology, Hebei General Hospital, Shijiazhuang, Hebei, People’s Republic of ChinaCorrespondence: Jianhui CaiDepartment of Surgery & Oncology, Hebei General Hospital, 348 West Heping Road, Shijiazhuang, Hebei 050051, People’s Republic of ChinaTel +86-13315124890Fax +86-0311-85511699Email jianhuicai201@163.comPurpose: Based on the continuous exploration of solid tumor immunotherapy, we focused on hepatocellular carcinoma with a high level of morbidity and mortality. We confirm the stability of mcDNA-based CAR T cell generating platform, and investigate the antitumor activity of CD44-CAR T cells against hepatocellular carcinoma both in vitro and in vivo.Materials and Methods: We fused anti-CD44 scFv structure with transmembrane domain and intracellular domain. Using a non-viral mcDNA vector to load CD44-CAR gene, then transfected the mcDNA-CD44-CAR into human T cells by electroporation. We exhibited the transfection efficacy of CAR T cells and the CD44 expression of tumor cell lines by flow cytometry. The antitumor efficacy of CD44-CAR T cells in vitro and in vivo was detected through CCK-8 and ELISA assays, and xenograft mouse models, respectively.Results: We obtained mcDNA-CD44-CAR with a high level of density after repeated extraction and purification. The expression efficacy of CD44-CAR in T cells was more than 50% after seven days electroporation and the phenotype of CD44-CAR T cells was no difference compared with normal T cells. For CD44-positive hepatocellular carcinoma xenograft mice, CD44-CAR T cells had stronger tumor growth suppression compared to normal T and mock T cells. The same results occurred on the in vitro experiments including cytokine secretion and cytotoxicity assays. H&E staining graphs revealed that CD44-CAR T cells did not induce side effects in xenograft mice.Conclusion: The strategy for generating CAR T cells targeting cancer stem cell antigens was efficient and concise. The mcDNA had superior transgene ability without virus-related adverse effects. CD44-CAR T cells had strong suppression capacity against hepatocellular carcinoma.Keywords: cancer immunotherapy, chimeric antigen receptor, nonviral vector, cancer stem cell antigen, tumor suppression
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- 2020
35. High ACTN1 Is Associated with Poor Prognosis, and ACTN1 Silencing Suppresses Cell Proliferation and Metastasis in Oral Squamous Cell Carcinoma
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Xie G, Zhao L, Chen Q, Tang D, Lu H, Cai J, and Chen Z
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oral squamous cell carcinoma ,lcsh:Therapeutics. Pharmacology ,proliferation ,lcsh:RM1-950 ,knockdown ,metastasis ,actinin alpha 1 ,poor prognosis - Abstract
Guo-feng Xie,1,* Luo-dan Zhao,2,3,* Qiang Chen,4,* Dong-xiao Tang,4 Qiong-yu Chen,4 Hong-fei Lu,4 Jia-rong Cai,5 Zheng Chen4 1Department of Radiation Oncology, Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou, Guangdong Province 510095, People’s Republic of China; 2Department of Oral and Maxillofacial Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province, People’s Republic of China; 3Key Laboratory of Malignant Tumor Gene Regulation and Target Therapy of Guangzhou Higher Education Institutes, Sun Yat-sen University, Guangzhou, Guangdong Province, People’s Republic of China; 4Department of Stomatology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, Guangdong Province, People’s Republic of China; 5Department of Urology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, Guangdong Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jia-rong CaiDepartment of Urology, The Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe Road, Guangzhou, Guangdong Province, People’s Republic of ChinaTel +86-20-82179727Fax +86-20-85253336Email caijiar@mail.sysu.edu.cnZheng ChenDepartment of Stomatology, The Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe Road, Guangzhou, Guangdong Province, People’s Republic of ChinaFax +86-20-85253336Email chenzh68@mail.sysu.edu.cnPurpose: Oral squamous cell carcinoma (OSCC) is a common malignancy of the oral cavity. As the survival rate of OSCC patients is low, it is crucial to explore new markers and therapeutic targets for early diagnosis of the disease. A high level of actinin alpha 1 (ACTN1) in patients could serve as an independent prognostic factor of acute myeloid leukemia. However, the role of ACTN1 in OSCC remains unclear. In the present study, we aimed to investigate the role of ACTN1 in OSCC.Methods: ACTN1 protein levels in tissues were determined by immunohistochemical (IHC) staining. The correlation of ACTN1 expression with clinicopathological features and prognosis was analyzed. Univariate and multivariate analyses were performed. The effect of ACTN1 knockdown on cell proliferation, migration, invasion, apoptosis, epithelial-mesenchymal transition (EMT), and the cell cycle was evaluated using Western blotting, Cell Counting Kit‑8 (CCK8) assays, flow cytometry analysis, transwell assays, wound-healing assays, and nude mouse models of subcutaneous xenograft and pulmonary metastasis.Results: Based on the total score of ACTN1 IHC staining analysis, ACTN1 expression was found to be low in 10 normal mucosal tissues, 48 normal mucosal tissues adjacent to OSCC, and 19 OSCC tissues, but high in 29 OSCC tissues. ACTN1 protein levels were significantly associated with the clinical stage and node metastasis, and a high ACTN1 protein level indicated poor prognosis. Moreover, ACTN1 expression was an independent predictor of poor prognosis of OSCC. Using in vitro assays, we found that ACTN1 knockdown could induce cell cycle arrest, promote apoptosis, and inhibit EMT and cell proliferation, migration, and invasion in the OSCC cell lines, SCC-15 and HSC-3. Moreover, ACTN1 knockdown inhibited subcutaneous tumor growth and pulmonary metastasis in vivo.Conclusion: ACTN1 levels were significantly associated with the clinical stage and node metastasis, and a high ACTN1 protein level indicated poor prognosis. Moreover, ACTN1 knockdown could suppress cell proliferation and metastasis of OSCC. Our results suggested that ACTN1 may serve as a diagnostic and prognostic marker of OSCC.Keywords: actinin alpha 1, poor prognosis, oral squamous cell carcinoma, knockdown, proliferation, metastasis
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- 2020
36. Catalpol Promotes the Proliferation and Differentiation of Osteoblasts Induced by High Glucose by Inhibiting KDM7A
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Cheng J, Xu H, Liu M, Cai J, Wang L, Hua Z, Wu X, Huo W, and Lv N
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RC581-951 ,kdm7a ,proliferation ,catalpol ,osteoblasts ,Specialties of internal medicine ,differentiation ,high glucose - Abstract
Jian Cheng,1,2,* Hai-yan Xu,3,* Ming-ming Liu,4 Jian-ping Cai,2 Lei Wang,2 Zhen Hua,2 Xiao-dong Wu,1 Wei-ling Huo,1 Nan-ning Lv4 1Department of Orthopedics, Xuzhou Central Hospital Affiliated to Nanjing University of Chinese Medicine, Xuzhou, Jiangsu 221009, People’s Republic of China; 2Institute of Traumatology & Orthopedics, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, People’s Republic of China; 3Department of Human Anatomy, Xuzhou Medical University, Xuzhou, Jiangsu 221004, People’s Republic of China; 4Department of Orthopedic Surgery, Lianyungang Second People’s Hospital, Lianyungang 222023, People’s Republic of China*These authors contributed equally to this workCorrespondence: Ming-ming LiuDepartment of Orthopedic Surgery, Lianyungang Second People’s Hospital, No. 41 Hailian East Road, Haizhou District, Lianyungang, Jiangsu 222023, People’s Republic of ChinaEmail mmliu89@126.comJian-ping CaiInstitute of Traumatology & Orthopedics, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, People’s Republic of ChinaEmail caijianpingcjp@126.comIntroduction: The protective effect of catalpol on diabetic osteoporosis (DOP) and its mechanism remain unclear. This study aimed to explore whether catalpol enhanced the proliferation and differentiation of MC3T3 cells induced by high glucose by inhibiting the expression of KDM7A.Methods: MC3T3 cells were induced by high glucose (HG) and treated with different concentrations of catalpol. The proliferation and mineralization abilities of MC3T3-E1 cells were determined by CCK-8 assay and Alizarin Red Staining, respectively. The expression of differentiation-related osteogenic proteins, KDM7A and related proteins of Wnt/β-catenin signaling pathway was analyzed by Western blot analysis. The alkaline phosphatase (ALP) activity was detected by ALP assay kits.Results: MC3T3-E1 cells induced by high glucose showed decreased proliferation and mineralization abilities and decreased ALP activity, which were all reversed by the treatment of catalpol. High glucose induction inhibited the expression of KDM7A, Total-β-catenin, Nuclear-β-catenin and p-GSK3β, which was reversed by the treatment of catalpol. And KDM7A interference up-regulated the expression of Total-β-catenin, Nuclear-β-catenin and p-GSK3β, which was down-regulated by KDM7A overexpression. Furthermore, the proliferation and mineralization abilities and ALP activity were improved when treated with KDM7A interference and decreased when treated with KDM7A overexpression. However, SKL2001 could improve the proliferation and mineralization abilities and ALP activity of MC3T3-E1 cells.Discussion: Catalpol promotes the proliferation and differentiation of osteoblasts induced by high glucose by regulating the Wnt/β-catenin signaling pathway through KDM7A.Keywords: catalpol, KDM7A, proliferation, differentiation, osteoblasts, high glucose
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- 2020
37. Correlation Between Diabetic Peripheral Neuropathy and Sarcopenia in Patients with Type 2 Diabetes Mellitus and Diabetic Foot Disease: A Cross-Sectional Study
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Yang Q, Zhang Y, Zeng Q, Yang C, Shi J, Zhang C, Ni X, Du Z, Tang Z, Hu J, Li X, Cai J, Li Q, and Cheng Q
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sarcopenia ,diabetic foot disease ,RC581-951 ,type 2 diabetes mellitus ,diabetic peripheral neuropathy ,correlation ,Specialties of internal medicine ,human activities - Abstract
Qin Yang, Yingxiao Zhang, Qinglian Zeng, Chan Yang, Jiale Shi, Chunlin Zhang, Xia Ni, Zhipeng Du, Ziwei Tang, Jinbo Hu, Xuemei Li, Jiahui Cai, Qifu Li, Qingfeng Cheng Department of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People’s Republic of ChinaCorrespondence: Qingfeng ChengThe First Affiliated Hospital of Chongqing Medical University, No. 1, Youyi Road, Yuanjiagang, Yuzhong District, Chongqing 400042, People’s Republic of ChinaTel +86-23-89011510Fax +86-23-890115540Email cqf19760516@163.comPurpose: The present study was designed to determine the relationships between sarcopenia and diabetic peripheral neuropathy (DPN) in patients with type 2 diabetes mellitus (T2DM) and diabetic foot disease (DFD) respectively.Patients and Methods: A total of 1104 patients with T2DM and 257 patients with DFD were included in the study, which was designed as a cross-sectional study. Body composition was assessed using dual-energy X-ray-absorptiometry (DXA). The diagnosis of sarcopenia was based on the Baumgartner criteria. DPN was assessed by Neuropathy symptom score (NSS) and Neuropathy disability score (NDS), and the severity of neuropathy was divided into non-neuropathy symptom (NS), Mild NS, Moderate NS and Severe NS according to NSS. Logistic regression analyses were carried out to determine the relations of sarcopenia and DPN in patients with T2DM and NSS in patients with DFD, respectively.Results: The prevalence of DPN was 80.0% in T2DM patients with sarcopenia and 70.3% in non-sarcopenia patients (P=0.007). Logistic regression analyses showed DPN was one of the independent risk factors for sarcopenia in T2DM patients (OR 1.564 [95% CI: 1.004, 2.435], P=0.048). The prevalence of DPN had no statistical significance in DFD patients with or without sarcopenia. However, the NSS of DFD patients with sarcopenia was higher than that of non-sarcopenia patients. In the multivariate logistic regression analysis, NSS was determined to be associated with sarcopenia in DFD patients (OR 1.387[95% CI: 1.074, 1.789], P=0.012). The appendicular lean mass (ALM) of DFD patients without NS was higher than patients with mild, moderate and severe NS (20.71± 2.73 vs 16.57± 3.62 vs 17.99± 3.54 vs 17.23± 3.29 Kg, P=0.028).Conclusion: DPN is an independent risk factor for sarcopenia in patients with T2DM and NSS is also independently correlated with sarcopenia in patients with DFD, with the latter being more obvious with the aggravation of neurological symptoms in DFD patients.Keywords: type 2 diabetes mellitus, diabetic peripheral neuropathy, diabetic foot disease, sarcopenia, correlation
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- 2020
38. DUB3 Facilitates Growth and Inhibits Apoptosis Through Enhancing Expression of EZH2 in Oral Squamous Cell Carcinoma
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Luo F, Zhou Z, Cai J, and Du W
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oral squamous cell carcinoma ,ubiquitin-specific processing proteases 17 ,bromodomain-containing protein 4 ,enhancer of zeste homolog-2 ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,lcsh:RC254-282 - Abstract
Fei Luo, Zunyan Zhou, Jun Cai, Wei Du Department of Oncology, First People’s Hospital of Jinzhou, Jinzhou 434000, People’s Republic of ChinaCorrespondence: Wei DuDepartment of Oncology, First People’s Hospital of Jinzhou, Tumor Hospital, No. 40, Jinlong Road, Jinzhou, Hubei Province 434000, People’s Republic of ChinaTel +86-18972161688Email drduwei@163.comBackground: Here, we probed the action mechanism of ubiquitin-specific processing proteases 17 (DUB3) in the evolution of oral squamous cell carcinoma (OSCC).Methods: The expression of genes were calculated by qRT-PCR, and proteins were assessed by Western blot and immunohistochemistry. The cells viability and proliferation were checked by MTT and EdU assay, respectively. Flow cytometry was implemented to detect the cell cycle and apoptosis. The activity of EZH2 gene promoter was measured by luciferase reporter assay. Co-immunoprecipitation assay was used to ensure the ubiquitination of bromodomain-containing protein 4 (BRD4). The cell apoptosis of tumor tissues was assessed by TUNEL assay.Results: DUB3 was overexpressed in OSCC tissues and cell lines, and negatively correlated with patient’s survival time. DUB3 downregulation could effectively curb OSCC cells viability and proliferation, promote cell apoptosis and the expression of cleaved-caspase-3, cleaved PARP and p21, while inhibit cyclin D1. Besides, DUB3 production was positivity correlated with enhancer of zeste homolog-2 (EZH2) and BRD4. BRD4 downregulation could repress DUB3-induced EZH2 production, and MG132 reversed DUB3 decreasing-mediated BRD4 downregulation. Downregulation of DUB3 promoted BRD4 ubiquitination. DUB3 promoted OSCC cells proliferation, while suppressing apoptosis via facilitating EZH2 production. At last, in vivo experiment indicated that the downregulation of DUB3 significantly inhibited the growth of xenograft tumor.Conclusion: In summary, we found that DUB3 enhanced OSCC cells proliferation and xenograft tumor growth, while inhibited their apoptosis via promoting BRD4-mediated upregulation of EZH2. Our study indicated that DUB3 may be an effective anti-cancer target for OSCC therapy.Keywords: oral squamous cell carcinoma, ubiquitin-specific processing proteases 17, enhancer of zeste homolog-2, bromodomain-containing protein 4
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- 2020
39. Novel cobalt‐free family of SrFe1‐xScx03‐δ perovskite materials for cathode applications in solid oxide fuel cells
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Li, Y., Larralde, A.L., Cai, J., Du, S., Troncoso, L., Fernández-Díaz, M.T., and Alonso, J.A.
- Abstract
Perovskites of composition SrFe1‐xScxO3‐δ (x = 0.05, 0.1, 0.2) were elaborated, characterized, and tested in solid‐oxide fuel cells (SOFC) as cathode materials. Porous materials were obtained by a solid‐state route. Thermogravimetric studies showed that the oxides eliminate oxygen as temperature increases. Neutron powder diffraction (NPD) experiments unveils that, at 800°C, the crystallographic structure of SrFe0.9Sc0.1O3‐δ contains δ = 0.51 (2) vacancies at oxygen sites, located at random in the cubic unit cell. The oxygen vacancies are essential for the oxygen diffusion, which is prerequisite for a MIEC‐type cathode. The electronic conductivity σ exhibits an insulator‐to‐metal transition at 420°C, reaching almost 200 S cm−1 for x = 0.05; σ was found to decrease as the Sc content increases. The cell performance was tested on electrolyte supported single cells, with LSGM electrolyte, fed by pure H2. The maximum power density reached by the Sc‐ferrite (x = 0.05) was of 340 mW cm−2 at 800°C.
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- 2020
40. Silencing of LncRNA Metastasis-Associated Lung Adenocarcinoma Transcript 1 Inhibits the Proliferation and Promotes the Apoptosis of Gastric Cancer Cells Through Regulating microRNA-22-3p-Mediated ErbB3
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Li X, Zhao J, Zhang H, and Cai J
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gastric cancer ,epidermal growth factor receptor 3 ,long non-coding rna metastasis-associated lung adenocarcinoma transcript 1 ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,microrna-22-3p ,lcsh:RC254-282 - Abstract
Xiaoning Li, 1–3 Jiangqiao Zhao, 4 Huiqing Zhang, 5 Jianhui Cai 1, 2 1Department of Surgery, Hebei Medical University, Shijiazhuang, Hebei 050017, People’s Republic of China; 2Department of General Surgery, Hebei General Hospital, Shijiazhuang, Hebei 050051, People’s Republic of China; 3Department of General SurgeryⅡ, Baoding First Central Hospital, Baoding, Hebei 071000, People’s Republic of China; 4Department of General Surgery, Cangzhou People’s Hospital, Cangzhou, Hebei 061000, People’s Republic of China; 5Department of Medical, Baoding First Central Hospital, Baoding, Hebei, People’s Republic of ChinaCorrespondence: Jianhui CaiDepartment of Surgery, Hebei Medical University, No. 361, East Zhongshan Road, Shijiazhuang, Hebei 050017, People’s Republic of ChinaTel +86-0311 8598 8902Email caijianhui25@163.comPurpose: This study aimed to investigate the regulatory effects and mechanisms of long non-coding RNA (LncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) on gastric cancer (GC) cells.Methods: The expression of MALAT1 was detected in GC tissues and two GC cell lines (SGC-7901 and BGC-823). MALAT1 was overexpressed and silenced in GC cells by the transfection of pcDNA-MALAT1 and siRNA-MALAT1, respectively. The proliferation and apoptosis of transfected cells, as well as the tumor volume and weight in mice injected with transfected cells were determined. After identifying the interaction between microRNA-22-3p (miR-22-3p) and MALAT1/epidermal growth factor receptor 3 (ErbB3), the effects of miR-22-3p/ErbB3 silencing on the proliferation and apoptosis of GC cells were evaluated.Results: MALAT1 was significantly upregulated in GC tissues and cells and negatively associated with the survival of GC patients. Overexpression of MALAT1 significantly promoted the proliferation and inhibited the apoptosis of SGC-7901 cells, while silencing of MALAT1 exerts contrary effects on BGC-823 cells. Silencing of MALAT1 also significantly inhibited the tumor growth in mice. In addition, MALAT1 negatively regulated its target miR-22-3p. Silencing of miR-22-3p reversed the anti-tumor effects of MALAT1 silencing on GC cells. MiR-22-3p negatively regulated its target ErbB3. Silencing of ErbB3 reversed the tumor-promoting effects of miR-22-3p silencing on GC cells.Conclusion: Silencing of MALAT1 inhibited the proliferation and promoted the apoptosis of GC cells through upregulating miR-22-3p and downregulating ErbB3.Keywords: long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1, gastric cancer, MicroRNA22-3p, epidermal growth factor receptor 3
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- 2020
41. Joint inversion of marine LOTEM and DED data from the Bat Yam coastal aquifer, offshore Israel
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Lieber, C., Haroon, A., Morbe, W., Cai, J., Lippert, K., Yogeshwar, P., and Tezkan, B.
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- 2022
42. $^{197}$Au($γ,\,xn;\,x\,=\,1\thicksim7$) Reaction Measurements using Laser-Driven Ultra-Bright Ultra-Fast Bremsstrahlung $γ$-Ray
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Wu, D., Zhang, J. Y., Lan, H. Y., Liu, J. X., Lv, J. F., Lu, H. G., Wu, X. Z., Zhang, H., Geng, Y. X., Zhao, Y. Y., Xu, J. Y., Cai, J., Yu, J. Q., Lin, C., Ma, W. J., Wang, H. R., Liu, F. L., He, C. Y., Guo, B., Zhang, G. Q., Wang, N. Y., Ma, Y. G., and Yan, X. Q.
- Subjects
FOS: Physical sciences ,Nuclear Experiment (nucl-ex) - Abstract
We present a new method for the measurements of photonuclear reaction flux-weighted average cross sections and isomeric yield ratios using laser-driven ultra-bright ultra-fast bremsstrahlung $γ$-ray, especially for photonuclear reactions with short half-life times. A 200 TW laser was used to generate stable near-monoenergetic electron beams via laser wakefield acceleration. The flux-weighted average cross sections and isomeric ratios of $^{197}$Au($γ,\,xn;\,x\,=\,1\thicksim7$) reactions were analyzed through activation measurements. The results showed good agreements with previous works and were compared with TALYS 1.9 calculations. The $^{197}$Au($γ$,\,7n)$^{190}$Au reaction cross sections were first achieved. This method offered a unique way of gaining insight into photonuclear reaction researches with short half-life times, which extremely lack experimental data.
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- 2022
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43. Nonlinear amplification of microwave signals in spin-torque oscillators
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Carpentieri, M., Zhu, K., Zhang, L., Cai, J., Verba, R., Giordano, A., Puliafito, V., Zhang, B., Finocchio, G., and and Z- Zeng
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- 2022
44. Generalized ℓ2−ℓp minimization based DOA estimation for sources with known waveforms in impulsive noise
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Dong, Y.-Y., Dong, C.-X., Liu, W., and Cai, J.
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- 2022
45. Mapping Wuhan
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Bekkering, H.C., Cai, J., Kuijper, J.A., Zhang, Ke, and Chen, Wei
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Wuhan ,China ,Delft School ,morphology ,history ,mapping - Abstract
Chinese cities have been expanding since the early 1980s under trends of rapid modernization, urbanization and globalization. Since then they have changed dramatically, and have in the process lost many of their traditional environments and spatial characteristics. Urban planners and designers have been and are facing unprecedented challenges in China. They not only have to learn to understand the constantly emerging new urban mechanisms, and seek balance among stakeholders, but they also need to cope with the political pressures and the changing context under often extreme time pressure. In such circumstances, future- and design-oriented analysis based on a ‘designerly’ way of thinking is useful—if not indispensable—for understanding the existing city and deciding on its transformations in a responsible and accountable way that is communicable among designers and with the public. This is especially so, in light of the growing awareness—also in China—of the value and importance of local urban identity, that is always—at least partially—based on history. In this ATLAS the Delft method of historical morphological analysis is applied to the city of Wuhan, valuing the importance of and finding meaning in the local urban identity of a city with a population over 11 million with a floating population of 14 million. The series of maps show the urban development, covering a century and a half.
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- 2022
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46. Flux Variations of Cosmic Ray Air Showers Detected by LHAASO-KM2A During a Thunderstorm on 10 June 2021
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LHAASO Collaboration, Aharonian, F., An, Q., Axikegu, Bai, L. X., Bai, Y. X., Bao, Y. W., Bastieri, D., Bi, X. J., Bi, Y. J., Cai, J. T., Cao, Zhe, Cao, Zhen, Chang, J., Chang, J. F., Chen, E. S., Chen, Liang, Chen, Long, Chen, M. J., Chen, M. L., Chen, S. H., Chen, S. Z., Chen, T. L., Chen, X. J., Chen, Y., Cheng, H. L., Cheng, N., Cheng, Y. D., Cui, S. W., Cui, X. H., Cui, Y. D., Dai, B. Z., Dai, H. L., Dai, Z. G., Danzengluobu, della Volpe, D., Duan, K. K., Fan, J. H., Fan, Y. Z., Fan, Z. X., Fang, J., Fang, K., Feng, C. F., Feng, L., Feng, S. H., Feng, X. T., Feng, Y. L., Gao, B., Gao, C. D., Gao, L. Q., Gao, Q., Gao, W., Gao, W. K., Ge, M. M., Geng, L. S., Gong, G. H., Gou, Q. B., Gu, M. H., Gu, F. L., Guo, J. G., Guo, X. L., Guo, Y. Q., Guo, Y. Y., Han, Y. A., He, H. H., He, H. N., He, S. L., He, X. B., He, Y., Heller, M., Hor, Y. K., Hou, C., Hou, X., Hu, H. B., Hu, Q., Hu, S., Hu, S. C., Hu, X. J., Huang, D. H., Huang, W. H., Huang, X. T., Huang, X. Y., Huang, Y., Huang, Z. C., Ji, X. L., Jia, H. Y., Jia, K., Jiang, K., Jiang, Z. J., Jin, M., Kang, M. M., Ke, T., Kuleshov, D., Li, B. B., Li, Cheng, Li, Cong, Li, F., Li, H. B., Li, H. C., Li, H. Y., Li, J., Li, Jian, Li, Jie, Li, K., Li, W. L., Li, X. R., Li, Xin, Li, Y. Z., Li, Zhe, Li, Zhuo, Liang, E. W., Liang, Y. F., Lin, S. J., Liu, B., Liu, C., Liu, D., Liu, H., Liu, H. D., Liu, J., Liu, J. L., Liu, J. S., Liu, J. Y., Liu, M. Y., Liu, R. Y., Liu, S. M., Liu, W., Liu, Y., Liu, Y. N., Long, W. J., Lu, R., Luo, Q., Lv, H. K., Ma, B. Q., Ma, L. L., Ma, X. H., Mao, J. R., Masood, A., Min, Z., Mitthumsiri, W., Nan, Y. C., Ou, Z. W., Pang, B. Y., Pattarakijwanich, P., Pei, Z. Y., Qi, M. Y., Qi, Y. Q., Qiao, B. Q., Qin, J. J., Ruffolo, D., Sáiz, A., Shao, C. Y., Shao, L., Shchegolev, O., Sheng, X. D., Shi, J. Y., Song, H. C., Stenkin, Yu. V., Stepanov, V., Su, Y., Sun, Q. N., Sun, X. N., Sun, Z. B., Tam, P. H. T., Tang, Z. B., Tian, W. W., Wang, B. D., Wang, C., Wang, H., Wang, H. G., Wang, J. C., Wang, J. S., Wang, L. P., Wang, L. Y., Wang, R., Wang, R. N., Wang, W., Wang, X. G., Wang, X. Y., Wang, Y., Wang, Y. D., Wang, Y. J., Wang, Y. P., Wang, Z. H. Wang. Z. X., Wang, Zhen, Wang, Zheng, Wei, D. M., Wei, J. J., Wei, Y. J., Wen, T., Wu, C. Y., Wu, H. R., Wu, S., Wu, X. F., W, Y. S., Xi, S. Q., Xia, J., Xia, J. J., Xiang, G. M., Xiao, D. X., Xiao, G., Xin, G. G., Xin, Y. L., Xing, Y., Xiong, Z., Xu, D. L., Xu, R. X., Xue, L., Yan, D. H., Yan, J. Z., Yang, C. W., Yang, F. F., Yang, H. W., Yang, J. Y., Yang, L. L., Yang, M. J., Yang, R. Z., Yang, S. B., Yao, Y. H., Yao, Z. G., Ye, Y. M., Yin, L. Q., Yin, N., You, X. H., You, Z. Y., Yu, Y. H., Yuan, Q., Yue, H., Zeng, H. D., Zeng, T. X., Zeng, W., Zeng, Z. K., Zha, M., Zhai, X. X., Zhang, B. B., Zhang, F., Zhang, H. M., Zhang, H. Y., Zhang, J. L., Zhang, L. X., Zhang, Li, Zhang, Lu, Zhang, P. F., Zhang, P. P., Zhang, R., Zhang, S. B., Zhang, S. R., Zhang, S. S., Zhang, X., Zhang, X. P., Zhang, Y. F., Zhang, Y. L., Zhang, Yi, Zhang, Yong, Zhao, B., Zhao, J., Zhao, L., Zhao, L. Z., Zhao, S. P., Zheng, F., Zheng, Y., Zhou, B., Zhou, H., Zhou, J. N., Zhou, P., Zhou, R., Zhou, X. X., Zhu, C. G., Zhu, F. R., Zhu, H., Zhu, K. J., and Zuo, X.
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High Energy Astrophysical Phenomena (astro-ph.HE) ,Nuclear and High Energy Physics ,High Energy Physics - Experiment (hep-ex) ,FOS: Physical sciences ,Astronomy and Astrophysics ,Astrophysics - High Energy Astrophysical Phenomena ,Instrumentation ,High Energy Physics - Experiment - Abstract
The Large High Altitude Air Shower Observatory (LHAASO) has three sub-arrays, KM2A, WCDA and WFCTA. The flux variations of cosmic ray air showers were studied by analyzing the KM2A data during the thunderstorm on 10 June 2021. The number of shower events that meet the trigger conditions increases significantly in atmospheric electric fields, with maximum fractional increase of 20%. The variations of trigger rates (increases or decreases) are found to be strongly dependent on the primary zenith angle. The flux of secondary particles increases significantly, following a similar trend with that of the shower events. To better understand the observed behavior, Monte Carlo simulations are performed with CORSIKA and G4KM2A (a code based on GEANT4). We find that the experimental data (in saturated negative fields) are in good agreement with simulations, assuming the presence of a uniform upward electric field of 700 V/cm with a thickness of 1500 m in the atmosphere above the observation level. Due to the acceleration/deceleration and deflection by the atmospheric electric field, the number of secondary particles with energy above the detector threshold is modified, resulting in the changes in shower detection rate., Comment: 18 pages, 11 figures
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- 2022
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47. A Possible 3-Year Quasi-Periodic Oscillation in γ-Ray Emission from the FSRQ S5 1044+71
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Wang, G. G., Cai, J. T., and Fan, J. H.
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High Energy Astrophysical Phenomena (astro-ph.HE) ,FOS: Physical sciences - Abstract
Variability is a typical observation feature of Fermi blazars, sometimes it shows quasi-periodic oscillation (QPO). In this work, we obtained 5-day binned light curves (with a time coverage of $\sim$ 12.9 yr) for S5 1044+71 based on Fermi LAT data, adopted five different methods: Date-compensated Discrete Fourier Transform (DCDFT), Jurkevich (JV), Lomb-Scargle Periodogram (LSP), a Fortran 90 program (REDFIT) and the Weighted Wavelet Z-transform (WWZ) to the $γ$-ray light curve, and found a possible QPO of 3.06 $\pm$ 0.43 yr at the significance level of $\sim3.6σ$. A binary black hole model including accretion model and dual-jets model is used to explain this quasi-periodic variability. We also estimated the Doppler factors and the apparent velocity for the two jet components. We speculate that this $γ$-ray quasi-periodic modulation suggest the presence of a binary supermassive black hole in S5 1044+71., 11 pages, 10 figures, accepted by APJ
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- 2022
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48. Prevalence and Risk Factors of Immunosuppressant Nonadherence in Heart Transplant Recipients: A Single-Center Cross-Sectional Study
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Zhang M, Zhou H, Nelson RS, Han Y, Wang Y, Xiang H, Cai J, Zhang J, and Yuan Y
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lcsh:R5-920 ,immunosuppressants ,risk factors ,adherence ,heart transplantation ,lcsh:Medicine (General) ,interventions - Abstract
Min Zhang,1,2,* Hong Zhou,2,* Ryan S Nelson,3 Yong Han,2 Yirong Wang,1,2 Hongping Xiang,2 Jie Cai,4 Jing Zhang,4 Yonghua Yuan1 1Department of Pharmacy, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Pediatrics, Children’s Hospital of Chongqing Medical University, Chongqing 400014, People’s Republic of China; 2Department of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People’s Republic of China; 3Department of Individualized Cancer Management, Moffitt Cancer Center, Tampa, FL 33612, USA; 4Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yonghua YuanDepartment of Pharmacy, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Pediatrics, Children’s Hospital of Chongqing Medical University, No. 136 Zhongshan Er Road, Yuzhong District, Chongqing 400014, People’s Republic of ChinaTel +86-023-68485161Email 400766@cqmu.edu.cnBackground: Immunosuppressant nonadherence (INA) has been shown to affect outcomes after solid organ transplantation. The aim of the present study was to determine the prevalence of INA in heart transplant recipients and the associated risk factors of INA.Methods: Adult heart transplant recipients who firstly received heart transplantation (discharged for at least 3 months) were consecutively enrolled. Immunosuppressant adherence was assessed using the Basel Assessment of Adherence with Immunosuppressive Medication Scale (BAASIS). INA was categorized into five domains of contributing factors (socio-demographic factors, transplant-related factors, healthcare system access factors, post-transplant treatment-related factors, and patient-related psychosocial factors). These factors were compared between adherent and nonadherent patients. The risk factors of INA were investigated by logistic regression analysis.Results: A total of 168 heart recipients were ultimately included. Among them, 69 (41.1%) recipients were revealed to be nonadherent. Logistic regression analysis indicated that INA was associated with monthly income
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- 2019
49. MTF2 Induces Epithelial-Mesenchymal Transition and Progression of Hepatocellular Carcinoma by Transcriptionally Activating Snail
- Author
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Wu TT, Cai J, Tian YH, Chen JF, Cheng ZL, Pu CS, Shi WZ, Suo XP, Wu XJ, Dou XW, and Zhang KM
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emt ,snail ,mtf2 ,progression ,hepatocellular carcinoma ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,lcsh:RC254-282 - Abstract
Tian-Tian Wu,* Jun Cai,* Yuan-Hu Tian, Jian-Fei Chen, Zhi-Lei Cheng, Chang-Sheng Pu, Wen-Zai Shi, Xiao-Peng Suo, Xian-Jia Wu, Xiao-Wei Dou, Ke-Ming Zhang Department of Hepatobiliary Surgery, Peking University International Hospital, Beijing 102206, People’s Republic of China*These authors contributed equally to this workCorrespondence: Ke-Ming ZhangDepartment of Hepatobiliary Surgery, Peking University International Hospital, No 1 Shengmingyuan Road, Zhongguancun Life Science Park, Changping District, Beijing 102206, People’s Republic of ChinaEmail kemingzhang2019@163.comBackground: Metal regulatory transcription factor 2 (MTF2) has been previously reported as a protein binding to the metal response element of the mouse metallothionein promoter, which is involved in chromosome inactivation and pluripotency. However, the function of MTF2 in tumor formation and progression has not yet been completely elucidated.Methods: The expression of MTF2 and clinicopathological characteristics were evaluated by hepatocellular carcinoma (HCC) tissue microarray of 240 specimens. The role of MTF2 on HCC progression was determined using MTT, crystal violet, and transwell assays. Tumor growth was monitored in a xenograft model, and intrahepatic metastasis models were established.Results: The expression of MTF2 was increased in HCC and strongly associated with the clinical characteristics and prognosis. Forced expression of MTF2 in HCC cells significantly promoted cell growth, migration, and invasion in vitro. In contrast, downregulation of MTF2 inhibited cell growth, migration, and invasion in vitro. Moreover, knock down of MTF2 suppressed tumorigenesis and intrahepatic metastasis of HCC cells in vivo. Mechanistically, MTF2 overexpression may promote growth and epithelial-mesenchymal transition processes of HCC cells by facilitating Snail transcription.Conclusion: MTF2 promotes the proliferation, migration, and invasion of HCC cells by regulating Snail transcription, providing a potential therapeutic candidate for patients with HCC.Keywords: MTF2, progression, hepatocellular carcinoma, EMT, Snail  
- Published
- 2019
50. Assessment of Plasmodium falciparum infection and fitness of genetically modified Anopheles gambiae aimed at mosquito population replacement
- Author
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Philpott J, Nikolai Windbichler, Sofia Tapanelli, George K. Christophides, Cai J, Inghilterra Mg, and Paolo Capriotti
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Genetics ,biology ,Transgene ,Anopheles gambiae ,Locus (genetics) ,Plasmodium falciparum ,Gene drive ,medicine.disease ,biology.organism_classification ,Genetically modified organism ,parasitic diseases ,medicine ,Genotyping ,Malaria - Abstract
Genetically modified (GM) mosquitoes expressing anti-plasmodial effectors propagating through wild mosquito populations by means of gene drive is a promising tool to support current malaria control strategies. The process of generating GM mosquitoes involves genetic transformation of mosquitoes from a laboratory colony and, often, interbreeding with other GM lines to cross in auxiliary traits. These mosquito colonies and GM lines thus often have different genetic backgrounds and GM lines are invariably highly inbred, which in conjunction with their independent rearing in the laboratory may translate to differences in their susceptibility to malaria parasite infection and life history traits. Here, we show that laboratory Anopheles gambiae colonies and GM lines expressing Cas9 and Cre recombinase vary greatly in their susceptibility to Plasmodium falciparum NF54 infection. Therefore, the choice of mosquitoes to be used as a reference when conducting infection or life history trait assays requires careful consideration. To address these issues, we established an experimental pipeline involving genetic crosses and genotyping of mosquitoes reared in shared containers throughout their lifecycle. We used this protocol to examine whether GM lines expressing the antimicrobial peptide (AMP) Scorpine in the mosquito midgut interfere with parasite infection and mosquito survival. We demonstrate that Scorpine expression in the Peritrophin 1 (Aper1) genomic locus reduces both P. falciparum sporozoite prevalence and mosquito lifespan; both these phenotypes are likely to be associated with the disturbance of the midgut microbiota homeostasis. These data lead us to conclude that the Aper1-Sco GM line could be used in proof-of-concept experiments aimed at mosquito population replacement, although the impact of its reduced fitness on the spread of the transgene through wild populations requires further investigation.
- Published
- 2021
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