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Resume Introduction Le stress occasionne par la gestion des appels de detresse recus au SAMU peut representer, pour les assistants de regulation medicale (ARM), un risque psychologique. Objectif Etudier les repercussions psychologiques sur les ARM. Methode Etude prospective, multicentrique, nationale, d’un mois aupres d’ARM de 13 SAMU par questionnaire electronique de cinq sections : caracteristiques de la population, typologie des appels, echelle PCL-5, echelle ProQOL, ressentis et retentissements du travail sur leur vie. Recherche des criteres associes a une symptomatologie evocatrice de trouble de stress post-traumatique (TSPT) complet defini par le DSM-5 et PCL-5 ≥ 34. Resultats Tous les ARM ont ete confrontes a la mort dont 129 (46 %) dans les sept derniers mois. Trente (11 %) presentaient une symptomatologie evocatrice d’un TSPT complet et 42 (15 %) celle d’un TSPT incomplet. Le groupe TSPT complet avait significativement plus d’ARM ayant au plus le baccalaureat (n = 22, 74 % vs n = 145, 57 % ; p = 0,04). Aucune autre difference n’etait significative. Le groupe TSPT complet developpait significativement (p Conclusion Chez 1/3 des ARM interroges, une symptomatologie evocatrice de TSPT complet ou incomplet etait retrouvee avec des processus psychotraumatiques specifiques. La formation semble etre une protection efficace. Elle doit etre renforcee, tout comme la prevention.

7 pags., 3 figs., 1 tab., States in the N = 35 and 37 isotopes 55,57Ca have been populated by direct proton-induced nucleon removal reactions from 56,58Sc and 56Ca beams at the RIBF. In addition, the (p, 2p) quasi-free single- proton removal reaction from 56Ca was studied. Excited states in 55K, 55Ca, and 57Ca were established for the first time via in-beam γ -ray spectroscopy. Results for the proton and neutron removal reactions from 56Ca to states in 55K and 55Ca for the level energies, excited state lifetimes, and exclusive cross sections agree well with state-of-the-art theoretical calculations using different approaches. The observation of a short-lived state in 57Ca suggests a transition in the calcium isotopic chain from single-particle dominated states at N = 35 to collective excitations at N = 37., We would like to thank the RIKEN accelerator and BigRIPS teams for providing the high intensity beams. T.K. acknowledges support by RIKEN Junior Research Associate Program. K.W. acknowledges support from the Spanish Ministerio de Ciencia, Innovación y Universidades RYC-2017-22007. RIUMF receives funding via a contribution through the National Research Council of Canada. J.D.H is further supported by NSERC under grants SAPIN-2018-00027 and RGPAS-2018-522453. VS-IMSRG computations were performed with an allocation of computing resources on Cedar at WestGrid and Compute Canada, and on the Oak Cluster at TRIUMF managed by the University of British Columbia department of Advanced Research Computing (ARC). N.S. and Y.U. acknowledge valuable support by “Priority Issue on post-K computer” and KAKENHI grant 20K03981 and 17K05433. C.B. was supported by the UK Science and Technology Facilities Council (STFC) through grants No. ST/L005816/1 and No. ST/V001108/1. SCGF calculations were per- formed by using HPC resources from GENCI-TGCC, France (Contract No. A009057392) and at the DiRAC DiAL system at the University of Leicester, UK, (funded by the UK BEIS via STFC Capital Grants No. ST/K000373/1 and No. ST/R002363/1 and STFC DiRAC Operations Grant No. ST/R001014/1). I.M. was supported by the RIKEN IPA program, F.B. by the RIKEN Special Postdoctoral Researche Program. D.S. acknowledges support from the European Regional Development Fund contract No. GINOP-2.3.3-15-2016-00034 and the National Research, Development and Innovation Fund of Hungary via Project No. K128947. K. I. H., D. K., and S. Y. P. acknowledge the support from the IBS grant funded by the Korea government (No. IBS-R031-D1). The work was further supported by JSPS KAKENHI Grant Nos. JP16H02179, JP18H05404, JP19H00679, and JP21H01114 and the Deutsche Forschungsgemeinschaft (DFG) under Grant No. BL 1513/1-1

Direct proton-knockout reactions of ^{55}Sc at ∼220 MeV/nucleon were studied at the RIKEN Radioactive Isotope Beam Factory. Populated states of ^{54}Ca were investigated through γ-ray and invariant-mass spectroscopy. Level energies were calculated from the nuclear shell model employing a phenomenological internucleon interaction. Theoretical cross sections to states were calculated from distorted-wave impulse approximation estimates multiplied by the shell model spectroscopic factors, which describe the wave function overlap of the ^{55}Sc ground state with states in ^{54}Ca. Despite the calculations showing a significant amplitude of excited neutron configurations in the ground-state of ^{55}Sc, valence proton removals populated predominantly the ground state of ^{54}Ca. This counterintuitive result is attributed to pairing effects leading to a dominance of the ground-state spectroscopic factor. Owing to the ubiquity of the pairing interaction, this argument should be generally applicable to direct knockout reactions from odd-even to even-even nuclei.

8 pags., 4 figs., 3 tabs., The nuclear structure of 51Ar, an uncharted territory so far, was studied by the (p,2p) reaction using γ-ray spectroscopy for the bound states and the invariant mass method for the unbound states. Two peaks were detected in the γ-ray spectrum and six peaks were observed in the 50Ar+n relative energy spectrum. Comparing the results to our shell-model calculations, two bound and six unbound states were established. Three of the unbound states could only be placed tentatively due to the low number of counts in the relative energy spectrum of events associated with the decay through the first excited state of 50Ar. The low cross sections populating the two bound states of 51Ar could be interpreted as a clear signature for the presence of significant subshell closures at neutron numbers 32 and 34 in argon isotopes. It was also revealed that due to the two valence holes, unbound collective states coexist with individual-particle states in 51Ar., We are very grateful to the RIKEN Nishina Center accelerator staff for providing the stable beam and to the BigRIPS team for the smooth operation of the secondary beams. The development of the MINOS device has been supported by the European Research Council through the ERC Grant No. MINOS-258567. F. B. was supported by the RIKEN Special Postdoctoral Researcher Program. K. O. acknowledges the support by Grant-in-Aid for Scientific Research JP16K05352. Y. U. acknowledges the support by Grant-in-Aid for Scientific Research 20K03981. Y. L. S. acknowledges the support of Marie Skłodowska-Curie Individual Fellowship (H2020-MSCA-IF-2015-705023) from the European Union and the support from the Helmholtz International Center for FAIR. H. N. L. acknowledges the support from the Enhanced Eurotalents program (PCOFUND-GA-2013-600382) co-funded by CEA and the European Union. T. A., C. L., D. R., H. T., V. W., L. Z., H. N. L., V. W. and A. O. acknowledge the support from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) Project No. 279384907-SFB 1245. R. B. G. acknowledges the support from the DFG under Grant No. BL 1513/1-1. Y. L. S. and A. O. acknowledge the support from the Alexander von Humboldt Foundation. B. D. L. and L. X. C. acknowledge the support from the Vietnam Ministry of Science and Technology under Grant No. ĐTCB.01/21/VKHKTHN. I. G. has been supported by HIC for FAIR and HRZZ under project No. 1257 and 7194. K. I. H., D. K. and S. Y. P. acknowledge the support from the NRF grant funded by the Korea government (No. 2017R1A2B2012382 and 2019M7A1A1033186). F. B. acknowledge the support from the RIKEN Special Postdoctoral Researcher Program. D. S. and Z. E. were supported by projects No. GINOP-2.3.3-15-2016-00034 and No. K128947. V. V. acknowledges support from the Spanish Ministerio de Economía y Competitividad under Contract No. FPA2017-84756-C4-2-P. V. W. and P. K. acknowledge the support from BMBF grants 05P15RDFN1 and 05P19RDFN1. P. K. acknowledges support from HGS-HIRe. This work was also supported by NKFIH (114454).

7 pags., 4 figs., 1 tab., Excited states in the N=40 isotone Ti were populated via the V(p,2p)Ti reaction at ∼200 MeV/nucleon at the Radioactive Isotope Beam Factory and studied using γ-ray spectroscopy. The energies of the 2 →0 and 4 →2 transitions, observed here for the first time, indicate a deformed Ti ground state. These energies are increased compared to the neighboring Cr and Fe isotones, suggesting a small decrease of quadrupole collectivity. The present measurement is well reproduced by large-scale shell-model calculations based on effective interactions, while ab initio and beyond mean-field calculations do not yet reproduce our findings. The shell-model calculations for Ti show a dominant configuration with four neutrons excited across the N=40 gap. Likewise, they indicate that the N=40 island of inversion extends down to Z=20, disfavoring a possible doubly magic character of the elusive Ca., We thank the RIKEN Nishina Center accelerator staff and the Bi-gRIPS team for the stable operation of the high-intensity Zn beam and for the preparation of the secondary beam setting. K.O. ac-knowledges the support by Grant-in-Aid for Scientific Research of the Japan Society for the Promotion of Science (JSPS) JP16K05352. A.P. is supported in part by the Ministerio de Ciencia, Innovación y Universidades (Spain), Severo Ochoa Programme SEV-2016-0597 and grant PGC-2018-94583. F.B. is supported by the RIKEN Spe-cial Postdoctoral Researcher Program. L.X.C. and B.D.L. would like to thank the Vietnam Ministry of Science and Technology (MOST) for its support through the Physics Development Program Grant No. ÐTÐLCN.25/18. I.G. has been supported by HIC for FAIR and Croatian Science Foundation under projects no. 1257 and 7194. D. So. was supported by the the European Regional Develop-ment Fund contract No. GINOP-2.3.3-15-2016-00034 and the National Research, Development and Innovation Fund of Hungary via Project No. K128947. V.V. acknowledges support from the Span-ish Ministerio de Economía y Competitividad under Contract No. FPA2017-84756-C4-2-P. K.I.H., D.K. and S.Y.P. acknowledge the sup-port from the National Research Foundation of Korea grant No. 2018R1A5A1025563 and 2019M7A1A1033186. The development of MINOS was supported by the European Research Council through the ERC Grant No. MINOS-258567. This work was also supported by the JSPS KAKENHI Grant No. 18K03639, MEXT as “Priority is-sue on post-K computer” (Elucidation of the fundamental laws and evolution of the universe), the Joint Institute for Computational Fundamental Science (JICFuS), the CNS-RIKEN joint project for large-scale nuclear structure calculations, Natural Sciences and Engineering Research Council (NSERC) of Canada, the Deutsche Forschungsgemeinschaft – Projektnummer 279384907 – SFB 1245, the PRISMA Cluster of Excellence, and the BMBF under Contracts No. 05P18RDFN1 and 05P19RDFN1. TRIUMF receives funding via a contribution through the National Research Council Canada. Com-putations were performed at the Jülich Supercomputing Center (JURECA)

In France, the emergency call center is called SAMU (service d'aide médicale d'urgence). The Medical Dispatcher Assistant (MDA) is the first responder and is exposed to first calls of distress and has a high risk of stress disorder.Psychological impact of emergency calls on MDA.National multicenter prospective study from January to August 2018 by electronic surveys, including all MDA of 13 SAMU, subdivided in 5 sections: population characteristics, PCL-5 scale (DSM-5) assessing post-traumatic stress disorder (PTSD), ProQOL assessing professional quality of life, call categories and an MDA's emotional perception, and work impacts on an MDA's quality of life. Univariate descriptive statistical analysis of the group with PCL-5≥34 (=complete PTSD group) and with PCL-534 (=group without complete PTSD).Of 400 MDA asked to be interviewed, 283 (71 %) replied of whom 72 % (205) were women and 28 % (79) men. Age groups: 9 % (25) for 18-25 yrs, 39 % (110) for 26-35 yrs, 31 % (89) 36-45 yrs, 15 % (43) 46-55 yrs and 6 % (16) for more than 56 yrs. All MDA reported having been exposed to death experience. For 46 % (129) the most recent traumatic event occurred within the last 7 months. 78 % (219) have reported intense fear, feeling helpless, or even sensed horror when answering the calls. 97 % (273) could talk about it with colleagues but only 64 % (180) with family. 72 % (203) felt lack of recognition at work. 78 % (220) had no knowledge about psycho-traumatic disorder. While 11 % (30) suffered symptoms suggestive of a complete PTSD, 15 % (42) an incomplete PTSD, 3 % (8) suffer burnout and 4 % (11) compassion fatigue, none reported secondary traumatic stress. The only significant difference (P0.05) between the two groups characteristics was on the education level. 74 % (22) of the MDA with a complete PTSD had a High School diploma or less. MDA with symptoms suggestive of complete PTSD developed significantly (P0.001) more stress reduction strategies (alcohol, drugs, medication) (13 % vs 2 %), had more food disorders (80.5 % vs 38 %), more sleeping problems (75.5 % vs 21 %), more anxiety (67 % vs 17 %), and more sick leaves (13 % vs 4 %) than the group without complete PTSD.Part of the surveyed MDAs showed symptoms suggestive of PTSD. The study highlights that MDAs is a vulnerable population, and PTSD prevention techniques should be systematically implemented for them. The study also highlights that a higher education level prevents the psycho traumatic process with its accompanying disorders.

Exclusive cross sections and momentum distributions have been measured for quasifree one-neutron knockout reactions from a ^{54}Ca beam striking on a liquid hydrogen target at ∼200 MeV/u. A significantly larger cross section to the p_{3/2} state compared to the f_{5/2} state observed in the excitation of ^{53}Ca provides direct evidence for the nature of the N=34 shell closure. This finding corroborates the arising of a new shell closure in neutron-rich calcium isotopes. The distorted-wave impulse approximation reaction formalism with shell model calculations using the effective GXPF1Bs interaction and ab initio calculations concur our experimental findings. Obtained transverse and parallel momentum distributions demonstrate the sensitivity of quasifree one-neutron knockout in inverse kinematics on a thick liquid hydrogen target with the reaction vertex reconstructed to final state spin-parity assignments.

Triple-negative breast cancer (TNBC) commonly develops resistance to chemotherapy, yet markers predictive of chemoresistance in this disease are lacking. Here, we define WNT10B-dependent biomarkers for β-CATENIN/HMGA2/EZH2 signaling predictive of reduced relapse-free survival. Concordant expression of HMGA2 and EZH2 proteins is observed in MMTV-Wnt10bLacZ transgenic mice during metastasis, and Hmga2 haploinsufficiency decreased EZH2 protein expression, repressing lung metastasis. A novel autoregulatory loop interdependent on HMGA2 and EZH2 expression is essential for β-CATENIN/TCF-4/LEF-1 transcription. Mechanistically, both HMGA2 and EZH2 displaced Groucho/TLE1 from TCF-4 and served as gatekeepers for K49 acetylation on β-CATENIN, which is essential for transcription. In addition, we discovered that HMGA2-EZH2 interacts with the PRC2 complex. Absence of HMGA2 or EZH2 expression or chemical inhibition of Wnt signaling in a chemoresistant patient-derived xenograft (PDX) model of TNBC abolished visceral metastasis, repressing AXIN2, MYC, EZH2, and HMGA2 expression in vivo. Combinatorial therapy of a WNT inhibitor with doxorubicin synergistically activated apoptosis in vitro, resensitized PDX-derived cells to doxorubicin, and repressed lung metastasis in vivo. We propose that targeting the WNT10B biomarker network will provide improved outcomes for TNBC. Significance: These findings reveal targeting the WNT signaling pathway as a potential therapeutic strategy in triple-negative breast cancer.

The first γ-ray spectroscopy of ^{52}Ar, with the neutron number N=34, was measured using the ^{53}K(p,2p) one-proton removal reaction at ∼210 MeV/u at the RIBF facility. The 2_{1}^{+} excitation energy is found at 1656(18) keV, the highest among the Ar isotopes with N>20. This result is the first experimental signature of the persistence of the N=34 subshell closure beyond ^{54}Ca, i.e., below the magic proton number Z=20. Shell-model calculations with phenomenological and chiral-effective-field-theory interactions both reproduce the measured 2_{1}^{+} systematics of neutron-rich Ar isotopes, and support a N=34 subshell closure in ^{52}Ar.

7 pags., 7 figs. -- XXIII International School on Nuclear Physics, Neutron Physics and Applications 22-28 September 2019, Varna, Bulgaria, Within the SEASTAR III campaign at the Radioactive Isotope Beam Factory, at the RIKEN Nishina Center, neutron-rich isotopes in the vicinity of 53K were produced from the fragmentation of the primary 70Zn beam on a 9Be target. After nucleon knockout reactions on the secondary liquid hydrogen MINOS target the known γ rays of the neutron-rich 55Sc isotope were observed (shown in this proceedings) and γ rays from 57,59Sc isotopes have been identified for the first time. The evolution of the occupied nucleon orbitals of these nuclei in the ground and excited state is investigated under the prism of the tensor force., We are grateful to the RIKEN Nishina Center accelerator staff for their work in the primary beam delivery and the BigRIPS team for preparing the secondary beams. This work was supported by the cooperation between TU Darmstadt and the GSI Helmholtz Center for Heavy Ion Research, by the Helmholtz Graduate School for Hadron and Ion Research for FAIR and its abroad program and the BMBF under grant No. 05P19RDFN1. D.S. was supported by projects No. GINOP2.3.3-15-2016-00034 and No. K128947.

We report on the first γ-ray spectroscopy of 51,53K produced via the 52,54Ca(p,2p) reactions at ∼250 MeV/nucleon. Unambiguous final-state angular-momentum assignments were achieved for beam intensities down to few particles per second by using a new technique based on reaction vertex tracking combined with a thick liquid-hydrogen target. Through γ-ray spectroscopy and exclusive parallel momentum distribution analysis, 3/2+ ground states and 1/2+ first excited states in 51,53K were established quantifying the natural ordering of the 1 d 3 / 2 and 2 s 1 / 2 proton-hole states that are restored at N = 32 and 34. State-of-the-art ab initio calculations and shell-model calculations with improved phenomenological effective interactions reproduce the present data and predict consistently the increase of the E(1/2 1 + ) - E(3/2 1 + ) energy differences towards N = 40.

Summary Background Intensive antiplatelet therapy with three agents might be more effective than guideline treatment for preventing recurrent events in patients with acute cerebral ischaemia. We aimed to compare the safety and efficacy of intensive antiplatelet therapy (combined aspirin, clopidogrel, and dipyridamole) with that of guideline-based antiplatelet therapy. Methods We did an international, prospective, randomised, open-label, blinded-endpoint trial in adult participants with ischaemic stroke or transient ischaemic attack (TIA) within 48 h of onset. Participants were assigned in a 1:1 ratio using computer randomisation to receive loading doses and then 30 days of intensive antiplatelet therapy (combined aspirin 75 mg, clopidogrel 75 mg, and dipyridamole 200 mg twice daily) or guideline-based therapy (comprising either clopidogrel alone or combined aspirin and dipyridamole). Randomisation was stratified by country and index event, and minimised with prognostic baseline factors, medication use, time to randomisation, stroke-related factors, and thrombolysis. The ordinal primary outcome was the combined incidence and severity of any recurrent stroke (ischaemic or haemorrhagic; assessed using the modified Rankin Scale) or TIA within 90 days, as assessed by central telephone follow-up with masking to treatment assignment, and analysed by intention to treat. This trial is registered with the ISRCTN registry, number ISRCTN47823388. Findings 3096 participants (1556 in the intensive antiplatelet therapy group, 1540 in the guideline antiplatelet therapy group) were recruited from 106 hospitals in four countries between April 7, 2009, and March 18, 2016. The trial was stopped early on the recommendation of the data monitoring committee. The incidence and severity of recurrent stroke or TIA did not differ between intensive and guideline therapy (93 [6%] participants vs 105 [7%]; adjusted common odds ratio [cOR] 0·90, 95% CI 0·67–1·20, p=0·47). By contrast, intensive antiplatelet therapy was associated with more, and more severe, bleeding (adjusted cOR 2·54, 95% CI 2·05–3·16, p Interpretation Among patients with recent cerebral ischaemia, intensive antiplatelet therapy did not reduce the incidence and severity of recurrent stroke or TIA, but did significantly increase the risk of major bleeding. Triple antiplatelet therapy should not be used in routine clinical practice. Funding National Institutes of Health Research Health Technology Assessment Programme, British Heart Foundation.

During January and February 2007 a European consumer test of eight new apple varieties and three standard ones was carried out in different European countries. The most common apple varieties were compared to new ones, focusing on consumer acceptance and the potential for increasing apple consumption. We sought to test the hypothesis that the introduction of new improved varieties would help to increase fruit consumption. Data from a sensory test involving 4290 consumers in seven different countries were analysed for the effects of variety and test country, age, gender and apple consumption habit of the consumer on eating quality acceptance. The results obtained showed a strong interaction between country and variety, although a group of four varieties (‘Gold Pink’, ‘CIVni’, ‘Cripps Pink’ and ‘Fuji’) formed part of a group of the varieties that were most accepted in all of the countries except Germany. Significant interactions between variety and age and variety and gender were observed, but their magnitudes were not considered to be of any practical significance. No statistically significant interaction was found between variety and apple consumption habit on eating quality acceptance.

We investigated the development of a low (T-type) and two high voltage-activated (N- and L-type) calcium channel currents in large diameter dorsal root ganglion neurones acutely isolated from embryonic mice using the whole-cell patch-clamp technique. The low and high voltage-activated barium currents (LVA and HVA) were identified by their distinct threshold of activation and their sensitivity to pharmacological agents, dihydropyridines and omega-conotoxin-GVIA, at embryonic day 13 (E13), E15 and E17-18, respectively, before, during and after synaptogenesis. The amplitude and density of LVA currents, measured during a -40 mV pulse from a holding potential of -100 mV, increased significantly between E13 and E15, and remained constant between E15 and E17-18. The density of global HVA current, elicited by 0 mV pulse, increased between E13 and E15/E17-18. The density of the N-type current studied by the application of omega-conotoxin-GVIA (1 microM) increased significantly between E13 and E15/E17-18. The use of the dihydropyridine nitrendipine (1 microM) revealed that the density of L-type current remained constant at each stage of development. Nevertheless, application of dihydropyridine Bay K 8644 (3 microM) demonstrated a significant slowing of the deactivation tail current between embryonic days 13 and 15, which may reflect a qualitative maturation of this class of calcium channel current. The temporal relationship between the changes in calcium channel pattern and the period of target innervation suggests possible roles of T-, N- and L-type currents during developmental key events such as natural neurone death and onset of synapse formation.

A consumer test carried out in 7 different European countries compared 3 standard apple varieties to 8 new ones. A total of 4290 consumers took part in the test. Data from this test was used to develop a preference map for apple. The preference map was constructed with 3 main dimensions (1 – sweetness, fruitiness, flowery attributes, 2 – acidity, firmness, 3 – juiciness and crispness). Consumers were segmented in 6 clusters according to their preferences. The 6 clusters were grouped into two main mega clusters A (68% of consumers) and B (32% of consumers). Megacluster A (Clusters 1, 2, 5 and 6) was characterized by preferring sweet apples. Clusters 2 and 5 (41% of consumers) liked sweet apples independently of their acidity and firmness and moderate positive values on dimension of juiciness and crispness. Cluster 1 (21% of consumers) had an optimal point in positive values of the sweetness dimension, moderate negative value for acidity and firmness and moderate positive value for juiciness and crispness. Cluster 6 (6% of consumers) besides preferring sweet varieties disliked acid-firm varieties. As to regard to megacluster B (Clusters 3 and 4) (32% of consumers), they preferred varieties that were acidic-firm and juiciy and crisp with values in the mid range of the sweetness dimension. In spite of the difficulties in translating preference dimensions into standard practical values for fruit quality and the fact of being a punctual measurement of consumer behaviour, this preference map could be of practical use of different actors on the fruit value chain like marketers and breeders.

We characterized toxin-insensitive calcium currents expressed by acutely dissociated embryonic dorsal root ganglion neurons. In the presence of 3 microM omega-conotoxin-GVIA, 3 microM nitrendipine and either 500 nM omega-agatoxin-IVA or 500 nM omega-conotoxin-MVIIC to inhibit N-, L- and P/Q-type currents, respectively, all neurons expressed two residual currents: a T-type and another which we referred to as toxin-resistant current. The toxin-resistant current (i) consisted of an inactivating and a sustained components, (ii) had a threshold of activation and a steady-state inactivation comprised between that of the T-type current and that of the other high-voltage-activated currents, (iii) had the same permeability for barium and calcium used as charge carriers, (iv) was highly sensitive to both cadmium and nickel; and (v) was insensitive to 500 microM amiloride which abolished the T-type at this concentration. The properties of the toxin-resistant current are very similar to those of the currents expressed in oocytes following injection of alpha(1E) subunits which we demonstrated to be present in these neurons. Therefore a component of the toxin-resistant current calcium channels in sensory neurons may be closely related to those calcium channels formed by alpha(1E) subunits.

Analysis of neuronal development has emphasized the importance of voltage-activated Ca2+ currents during the initial period of differentiation. We investigated non-N, non-L Ba2+ currents through Ca2+ channels in freshly dissociated large diameter embryonic mouse dorsal root ganglion neurons using the whole-cell patch-clamp technique. Two types of omega-agatoxin IVA-sensitive currents were clearly distinguished at embryonic day 13: a sustained P-type current blocked selectively at 30 nM (IC50 = 3nM) and an inactivating Q-type current blocked in the range 50-500 nM (IC50 = 120nM). The P-type Ca2+ current disappeared at day 15 whereas the Q-type Ca2+ current increased two- to three-fold during the same embryonic period. In contrast, the contribution of the non-L, non-N, omega-agatoxin IVA-resistant current (R-type) was constant during this developmental span. In conclusion, our results clearly show that P- and Q-type Ca2+ currents are differentially expressed during ontogenesis in large diameter dorsal root ganglion neurons. The developmental change, which occurs during the period of target innervation, could be related to specific key events such as natural neuron death and onset of synapse formation.

The aim of this study was to gain information concerning apple and peach consumption frequency within different European countries in relation to age and gender. The survey was a part of a complex experiment with the aim of evaluating consumers’ preferences towards new varieties, and the data is based on the self-reported declarations of respondents, male and female, between 15 and 70 years old. 4271 consumers from 7 European countries were invited to supply information about their apple consumption habits, whereas 499 respondents from 5 countries answered questions relating to frequency of peach and nectarine consumption. In both, the apple and the peach surveys, data analysis of declared intake showed significant differences between nationalities. The highest apple consumption was in Poland, where over 55% declared a consumption of more than 5 apples per week. In comparison, Italian consumers most often indicated eating 3–5 apples per week (39.3%). The lowest apple consumption was in the Netherlands and Spain. In the case of peaches, the highest consumption was indicated in France where 48% of respondents declared a peach consumption of 3–5 per week with 40% eating more than 5 fruits per week. The lowest peach intake was declared in Germany. Irrespective of country women were shown to eat more apples that men. Furthermore, the group of older people (61–70 years) consume apples more often than the adult group (36–60), while within the youngest group of consumers (16–35) eating apples was not at all popular. As with apples females declared a higher peach consumption, and again significantly lower fruit consumption by the youngest group (16–35) was indicated. Although the availability of fruit at the market remains a prime factor in determining apple and peach consumption, our survey confirmed the trends of declining this popular fruit intake by the younger generation, as well as the persistent tendency of lower frequency of fruit consumption among men than women.

International audience; NH3 and NO have been added to low pressure, premixed laminar CH4-O2 flames of different equivalence ratios. The profiles of concentrations of major radicals and stable species have been obtained, using a molecular beam sampling technique coupled with a mass spectrometer. Laser-induced fluorescence has been used to measure CH radicals. Absorption spectroscopy has been applied to OH radical and temperature measurements. The behaviours of NO and HCN are mainly discussed, and the whole experimental result compared to complete flame structure computations. Using a recent reaction mechanism described by Miller, we notice that pollutants NO and HCN are generally underestimated by computations. Another mechanism, proposed by Bian et al., for nitrogen oxidation has been tested. Sensitivity and reaction path analysis made it possible to identify the reactions which would require modifications, according to a review of recently published kinetic values, in order to get a better fit between the experimental profiles and the computed ones.

Background At this time, no predictive factor for response to biotherapy in RA patients is available in the daily practice. Objectives The objective is to determine predictive factors for the first biological maintenance in rheumatoid arthritis (RA) treatment. Methods From our database including 663 RA patients, 289 RA patients were received their first biotherapy between 2000 and 2009. Among these 289 RA patients, we selected 2 subsets of patients. In the first group, RA patients received the same biological therapy for at least 3 years (n=56). In the second group, RA patients required at least 3 different biological therapies over the 3 years following the first biological (n=36). Demographic parameters (age, sex, smoking, body mass index), RA activity (DAS28), biological parameters, DMARDs and glucocorticoid therapy were recorded. Chi-squared statistical analysis and odds ratios (OR) were performed. Results Due to the follow-up of three years, TNFa blockers were mainly used as first biotherapy (Etanercept, adalimumab, or infliximab) in 93%. Biotherapies were equally distributed in both groups. Demographic parameters and disease ac tivity were similar in the two groups. In the same way, biological parameters of RA severity were not different in both groups. However, combination of DMARDs before starting the first biotherapy was associated with requirement to switch frequently biotherapy compared to the first goup (OR = 4.2; 95%CI: 1.193 to 14.79; p Conclusions Our study shows that the presence of methotrexate at the starting time of a biotherapy is a factor for maintenance of the first biological therapy for at least three years. However, our result is mainly relevant for TNFa blockers which was the mainly biotherapy used at the first line. Disclosure of Interest None Declared

As relatively moderate-priced advanced ceramics became available on the market in the 80s, this opened up an interesting potential for fossil fuel-fired burner systems. When the combustion air in burners, radiant tubes and exchangers is preheated by recycled energy, certain components are subjected to higher temperatures.

A propos of the beginning of a serious psychotic disorder at the abatement of a cerebral vascular accident, clinical, paraclinical and psychometric developments during one year after treatment permits an analysis of the relation between the syphilitic meningo-encephalitis and the mental disorder.

Le choix de privilégier le cas des inventions hydrauliques dans l’Antiquité romaine découle de plusieurs constats, à des degrés de lecture différents. D’une part, ces inventions apparaissent, dans les sources écrites du moins, mais aussi à travers les vestiges archéologiques, comme un héritage technique de la Grèce et de l’Orient : c’est donc s’interroger sur leur statut d’inventio, en tant que création résolument nouvelle, et plus généralement, sur la nature des progrès techniques de la Rome...