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Protocol manuscript describing tibial nerve transection rat model of neuropathic pain. Published by Workpackage 2 of the EuroPain project. The EuroPain project has received support from the Innovative Medicines Initiative Joint Undertaking under grant agreement n° 115007, resources for which are composed of financial contribution from the European Union's Seventh Framework Programme (FP7/20072013) and EFPIA companies’ in kind contribution.This is a unique public-private partnership designed by the European Commission and European Federation of Pharmaceutical Industries and Associations (EFPIA). It is a pan-European collaboration that brings together large biopharmaceutical companies, small- and medium-sized enterprises (SMEs), patient organisations, academia, hospitals and public authorities.