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This paper considers the development of a technique to increase the biological value of milk and reduce the manifestations of allergic symptoms when it is consumed. The object of the study is a technique for enriching A2 milk. When digesting A2 milk, β-casomorphine-7 is not formed, which negatively affects the physiology of the gastrointestinal tract, cardiovascular, nervous, and endocrine systems. It is recommended to use milk, to which biologically active substances are added, so the question of its enrichment is relevant. Under industrial conditions, artificial vitamin and mineral complexes are used to enrich milk. Given that natural and synthetic substances have different effects on the human body, it is proposed to use carrot processing derivatives (Daucus carota) as a natural enrichment additive. The use of carrot powders improves the amino acid spectrum of milk. The mass concentration of amino acids in A2 milk enriched with powder from carrot peels was 4.87 g/100 g. The highest concentration found was, g/100 g: glutamic acid (0.84), proline (0.50), aspartic acid (0.42), leucine (0.41), and valine (0.35). It was established that the use of 200 g of such milk provides the daily needs of the body in essential amino acids: threonine, leucine, and phenylalanine (by 16%), methionine (by 4%), isoleucine (by 14%), lysine (by 18%), valine (by 20%). It was found that A2 milk enriched with powder from whole carrot roots has a higher content of carotenoids (0.1068mg/100ml), providing the body's need for them by 1.4%. Such milk can be an additional source of vitamin A produced in the human body. It is recommended to use powder from whole carrot roots for the enrichment of A2 milk under industrial conditions.

The aim of the work is to study the medical and biological properties of eggplant powders. Eggplant food powder is a raw material, an important characteristic of which is its chemical composition, in particular dietary fibers (pectin and fiber). Eggplant powders obtained by infrared drying of raw materials with subsequent grinding to 0.41/0.43 mm were used for the research. The low degree of esterification gives the powders increased sorption and detoxification properties for a number of heavy metal cations. It has been established that the ability of eggplant powder to bind heavy metals reaches 40‒50 %, a detoxification effect occurs, exogenous and endogenous poisons are adsorbed, and the putrefactive intestinal microflora decreases. It was established that the binding effect is influenced by the amount of protopectin and the pH of the medium, which is determined by the content of organic acids in eggplant powders. The obtained results confirm the complex-forming ability of the powders. Adding eggplant powder to the diet of rats reduces cholesterol by an average of 12 %. 10 % content of eggplant powder in the diet reduces the content of lipoproteins in the blood (up to 3.2 %), cholesterol (up to 1.9 %). Medical and biological studies establish the positive effect of raw materials on the animal body and determine the expediency of using a functional ingredient. In general, eggplant powder can be used as an effective supplement to reduce cholesterol and lipoproteins, which does not affect the main physiological systems of the body. The prospect of further research is an in-depth study of the chemical composition of raw materials, the development of recipes for dishes using eggplant powders.

Hammour fish (grouper fish) are known to be of great nutritional value for human consumption, as their protein has a high biological value and contains all the essential amino acids. Grouper fish are also a good source of minerals, vitamins, and fats that contain essential fatty acids. Thus, the current study aims to know the effect of different proportion of hummer fish on biochemical and histopathological changes of hyperglycemic rats. Twenty-four (24) Sprague Dawley-strain male albino rats, which weighed 150 ± 10 g, were divided into four groups. One group served as the negative control (normal), while the others were rendered diabetic using alloxan. One of the diabetic groups was considered the positive control and fed a standard diet, whereas the remaining two groups were fed with a 20% and 25% hammour fish diet for 28 days. At the end of the experiment, blood samples were taken from all the rats, and their organs were removed and subjected to biochemical analysis. The results indicated that the group fed with the 25% hammour fish diet exhibited significantly lower levels of liver, kidney, and heart damage, along with lower levels of serum glucose, total cholesterol, triglycerides, LDL, GOT, GPT and ALP, as compared to the positive control. The urea and creatinine levels were significantly higher for the rats that were fed the 20% hammour fish diet than for those in the positive control. The histopathological study of the heart showed a slight improvement of the heart tissues with the increase of hammour fish intake compare to the positive control, while kidney of rat from group 4, which were fed 25% hammour fish, showed granularity of epithelial lining glomerular tufts.

Nowadays, as the pandemic has reshaped snacking behaviors, and consumers have become more health-conscious, the need for the incorporation of “healthy snacking” in our diets has emerged. Although there is no agreed-upon definition of “healthy snacking”, dietary guidelines refer to snack foods with high nutritional and biological value. The aim of this study was to chemically characterize and determine the nutritional value of an innovative UVB-irradiated and baked snack from Pleurotus eryngii mushrooms. P. eryngii is an edible mushroom native to the Mediterranean basin. We applied proximate composition, amino acids, fatty acids, vitamins, and macro and trace elements analyses. Also, we computed indices to assess the nutritional quality of food, and we evaluated the sensory characteristics of the mushroom snack. We found high nutritional, consumer, and biological values for the snack. More specifically it was low in calories, high in fibre and protein, low in lipids, without added sugars, and high in ergosterol and beta-glucans. Additionally, it had some vitamins and trace elements in significant quantities. Its NRF9.3 score was considerably high compared to most popular snacks, and the snack exhibited high hypocholesterolemic and low atherogenic and thrombogenic potentials. In conclusion, as a result of UVB-irradiation and baking of P. eryngii mushrooms, the snack’s nutritional and biological value were not affected; instead, it provided a “healthy snacking” option.

Dietary protein allowances comprise the upper limits of the range of individual metabolic demands adjusted for digestibility and biological value. The maintenance demand includes well defined obligatory and less understood adaptive components. Demands for growth, pregnancy, and lactation are well understood, although factorial models of protein requirements assume low efficiency of dietary protein utilization and may overestimate dietary allowances for children, pregnant, and lactating women. The amino acid profile of the maintenance component is poorly defined but contains fewer indispensable amino acids than that for growth (the tissue protein pattern). Thus protein quality is less important for humans than growing animals.

This paper considers the development of a technique to increase the biological value of milk and reduce the manifestations of allergic symptoms when it is consumed. The object of the study is a technique for enriching A2 milk. When digesting A2 milk, β-casomorphine-7 is not formed, which negatively affects the physiology of the gastrointestinal tract, cardiovascular, nervous, and endocrine systems. It is recommended to use milk, to which biologically active substances are added, so the question of its enrichment is relevant. Under industrial conditions, artificial vitamin and mineral complexes are used to enrich milk. Given that natural and synthetic substances have different effects on the human body, it is proposed to use carrot processing derivatives (Daucus carota) as a natural enrichment additive. The use of carrot powders improves the amino acid spectrum of milk. The mass concentration of amino acids in A2 milk enriched with powder from carrot peels was 4.87 g/100 g. The highest concentration found was, g/100 g: glutamic acid (0.84), proline (0.50), aspartic acid (0.42), leucine (0.41), and valine (0.35). It was established that the use of 200 g of such milk provides the daily needs of the body in essential amino acids: threonine, leucine, and phenylalanine (by 16 %), methionine (by 4 %), isoleucine (by 14 %), lysine (by 18 %), valine (by 20 %). It was found that A2 milk enriched with powder from whole carrot roots has a higher content of carotenoids (0.1068 mg/100 ml), providing the body's need for them by 1.4 %. Such milk can be an additional source of vitamin A produced in the human body. It is recommended to use powder from whole carrot roots for the enrichment of A2 milk under industrial conditions., Дослідження присвячене розробці способу підвищення біологічної цінності молока та зменшенню проявів алергічних симптомів при його вживанні. Об’єктом дослідження є спосіб збагачення молока А2. При перетравленні молока А2 не утворюється β-казоморфін-7, який негативно впливає на фізіологію шлунково-кишкового тракту, серцево-судинну, нервову та ендокринну системи. Рекомендовано вживати молоко, до якого додано біологічно активні речовини, тому питання його збагачення є актуальним. У промислових умовах для збагачення молока використовуються штучні вітамінні та мінеральні комплекси. Враховуючи, що природні та синтетичні речовини по-різному діють на людський організм, в якості натуральної збагачуючої добавки запропоновано використовувати похідні переробки моркви (Daucus carota). Використання морквяних порошків покращує амінокислотний спектр молока. Масова концентрація амінокислот у молоці А2, збагаченому порошком із шкірок моркви, становила 4,87г/100г. Виявлено найбільшу концентрацію, г/100г: глутамінової кислоти (0,84), проліну (0,50), аспарагінової кислоти (0,42), лейцину (0,41), валіну (0,35). Встановлено, що вживання 200г такого молока забезпечує добові потреби організму у незамінних амінокислотах: треоніні, лейцині та фенілаланіні (на 16%), метіоніні (на 4%), ізолейцині (на 14%), лізині (на 18%), валіні (на 20%). Встановлено, що молоко А2, збагачене порошком із цільних коренеплодів моркви, має вищій вміст каротиноїдів (0,1068мг/100мл), забезпечуючи потребу організму в них на 1,4%. Таке молоко може бути додатковим джерелом вітаміну А, виробленим у людському організмі. Рекомендовано для збагачення молока А2 у промислових умовах використовувати порошок із цільних коренеплодів моркви

Carotenoids are the pigments present in plants, animals, and microorganisms which are responsible for a broad variety of colors found in nature. Their capacity as antioxidants mainly established their marketable success as health, food, and feed supplements, and cosmetics components. Currently, chemical synthesis dominates the worldwide market; however, due to the high biological value of natural carotenoids, the production scheme is moving towards microbial production as a profitable alternative.

Flaxseed (Linum usitatissimum) is chiefly farmed for oils and as a fibre crop. For the formation of flaxseed protein isolate (FPI) from unutilised flax meal, the analysis of amino acid profiling, nutritional profiling, micronutrient and macronutrient characterisation has very limited studies, which raise the interest in complete profiling of FPI. In this study, nutritional parameters such as leucine/isoleucine ratio, protein efficiency ratios, essential amino acid index, biological value and nutritional index confirmed that flaxseed protein isolate has a wide potential as a valuable food ingredient for the nutritional and food industry. Our findings suggested that FPI obtained at pH 12 exhibited a 43.25% protein recovery yield and an extraction yield of 17.2%. The first and second limiting amino acids in samples were leucine and threonine as per FAO/WHO scoring patterns. The characteristic amino acid profiling features of FPI signified that a valuable amount of non-polar amino acids (528.7 ± 0.01 mg/g), sulphur-containing amino acids (80.5 ± 0.01 mg/g) and aromatic amino acids (92.7 ± 0.03 mg/g) were present. A substantial amount of major minerals were also present in FPI. The experiments showed that protein isolate also contain traces of total sugars (1.18%), reducing sugars (0.10%) and polyphenols (1.90 µg/100 g). A great proportion of trace minerals such as Fe, Mo, Se, Cr, Zn, Cu and Mn were also detected. The morphological analysis of FPI was also analysed. The findings suggested that formulations of supplements from FPI will be a propitious tool to counter hidden hunger and poverty.

Las sopas son uno de los alimentos más importantes para los mexicanos. Sin embargo, por su alto contenido de carbohidratos, poca fibra y proteína, se caracterizan por estar desbalanceadas nutrimentalmente. El hongo Pleurotus incrementa la calidad nutricional de los alimentos (sopa de vegetales, snacks y pan de caja, por ejemplo). Las pastas son de bajo valor biológico por carecer de lisina; en cambio, Pleurotus columbinus posee aminoácidos esenciales, por lo que, al elaborar una pasta en combinación con este hongo, se obtendría una mayor calidad nutricional. Este trabajo muestra que la proteína de las pastas se incrementó en porcentajes de 32.15 % hasta 79.27 %, no hubo cambio en las propiedades físicas (tiempo de cocimiento y absorción de agua), a excepción de la desintegración, que se incrementó de 6.4 % hasta 8.5 %. La harina del hongo incrementa la cantidad proteínica de la pasta, lo cual ayudaría a mejorar la alimentación de la población.

Introduction. People with gastrointestinal disorders should have a sparing diet with a balanced chemical and amino acid composition including all essential components. Based on formulations of meat products, we identified a number of essential nutritional components that could improve the diet for gastrointestinal pathologies. In this study, we aimed to develop a formulation for cooked sausage enriched with deficient essential nutrients. Study objects and methods. Our study object was cooked sausage. First, we analyzed the diet for people with gastrointestinal disorders. Then, we formulated a meat-based product (cooked sausage), determined its chemical and amino acid compositions, as well as vitamin and mineral contents, and assessed the balance of amino acids. Finally, we evaluated the biological value and safety of the formulated sausage on laboratory mice. Results and discussion. The chemical and amino acid compositions of a daily gastrointestinal diet in medical institutions revealed a deficiency of some water-soluble vitamins, vitamin A, calcium, magnesium, and iron, as well as an imbalance of amino acids. To replenish the deficiency, we formulated a meat-based product composed of trimmed beef and pork, beef liver, egg mix, food gelatin, chitosan succinate, rice flour, and soy fortifier. The product was classified as a meat and cereal cooked sausage of grade B, in which most amino acids were used for anabolic purposes. Its daily portion of 100 g eliminated the deficiency of potassium and iron, almost completely replenished magnesium, calcium, and vitamin A, as well as reduced the deficiency of dietary fiber by 4.8 g. The cytological studies of the blood of laboratory animals, whose basic diet contained the formulated sausage, proved its high biological value and safety. Conclusion. We found that the formulated meat and cereal sausage can be included in the diet for patients with gastrointestinal diseases and used in medical institutions to eliminate the deficiency of essential nutrients.

The safety of plant-based food with an herbal origin is a priority for producers and final consumers these days. The interest in the high biological value of the final food products enriched with herbal ingredients is rising. We focused on the study of physico-chemical composition and antioxidant activity of two kinds of grape juice with medicinal plant addition in our study. We used 2 varieties of grapes - Welschriesling and Cabernet Sauvignon, six species of medicinal plants - Calendula officinalis L., Ginkgo biloba, Thymus serpyllum, Matricaria recutita, Salvia officinalis L., and Mentha aquatica var. citrata in our experiment. There were14 samples prepared, two of them were control samples and 12 samples were treated with medicinal plants. We tested each of the selected parameters triplicate with an interval of one week. We evaluated the results statistically in 4 levels of significance p

Purpose The purpose of this study is to assess the protein (biological) quality of pecan and pine nuts supplemented diets in male Wistar albino rats. Design/methodology/approach A randomized and controlled study (45 d) was conducted in male Wistar albino rats (n = 60) comprising six groups, namely, control (Cc), control with 1% cholesterol (CC1), Pecan group (Pe), Pecan group supplemented with 1% cholesterol (Pi1), Pine group supplemented with 1% cholesterol (Pi1). Protein quality of supplemented diet/s was assessed in terms of various biological indices. Findings Pe and Pi groups exhibited normal growth when compared with Cc. Despite showing higher feed and protein intake, Pe and Pi groups exhibited significantly (P = 0.023) lower weight gain than Cc. There was no significant difference between the protein efficiency ratio (3.8 ± 0.2 and 3.7 ± 0.2), apparent protein digestibility (81.5 ± 2.2 and 80.5 ± 2%), true protein digestibility (90.6 ± 1.1 and 88.5 ± 3.5%) and biological value (BV) (75.6 ± 0.9 and 72.6 ± 0.9%) of Pe and Pi groups, respectively. Pe group presented considerably higher net protein utilization (68.5 ± 1.4 vs 64.4 ± 0.8%) and protein retention efficiency (34.8 ± 1.1 vs 31.2 ± 1.3%) than Pi group. Overall, pecan-based diet displayed superior biological quality over pine nut. Antinutrients present in the nuts did not show any major negative effect on the growth, digestibility and bioavailability of the nutrients in rats. BV ranging from 72.6% to 75.6% suggested a satisfactory quality of experimental nut-based diets. Research limitations/implications Further investigation of pecan and pine nuts consumption to examine the potential benefits and associated mechanisms of action is warranted which would aid in a better understanding and to establish nutritional recommendations. Originality/value Overall, the exploitation of pecan and pine nut flours for the development of value-added food products is favorable from a nutritional point of view.

Dietetic therapy plays an important role in prevention and treatment of non-communicable, alimentary-dependent diseases including the bile duct diseases and chronic liver injury. The medico-biological requirements for the composition and quality of specialized semi-finished products for prevention and treatment of liver diseases are formulated and formalized in the paper. Using the methods of the computer design, the recipe of the specialized minced meat semi-finished product from horse meat, which is nutrient adequate to specific features of nutrition of patients with liver diseases, was developed and optimized. The experimental recipe of minced semi-finished products consists of horse meat of the second grade, soy protein fiber, soy protein isolate, textured soy flour, milk thistle oil meal, chicken eggs, soybean oil, salt, onion, vitamins B1, B2 and E, magnesium and zinc salts. The biological value of the obtained product is characterized by the high indices of the amino acid balance (Cmin=0.99, σс =0.85, U=6.27) and fatty acid balance of lipids ((RLi) I = 1…3 = 0.9 and (RLi) I = 1…6 -0.77). The content of dietary fibers, vitamins B1 and B2 as well as mineral salts of magnesium and zinc in the semi-finished product corresponds to the medico-biological requirements. The effectiveness of the developed product was confirmed in the experiment of the laboratory mice with simulated liver cirrhosis. The results of the assessment on the laboratory animals show that the developed product positively influences the processes of metabolism in the mouse body. The coefficient of protein assimilation in the group of animals fed with the specialized product was 25% higher than in animals received the standard vivarium diet. The biochemical blood analysis showed that intake of the developed product reduced the content of bilirubin, cholesterol, AST and ALT in the animal body by 34.5%, 8.2%, 11.0% and 12.5%, respectively.

The study was performed to investigate protein digestibility and utilization in an F2 cross (M2-F2 cross) between the selected Fat (F) line and an M2 congenic line. The congenic M2 line carried the Fob3b2 quantitative trait locus (QTL) from the selected Lean (L) line previously shown to contain the Tst gene with leanness, anti-diabetic and resistance to diet-induced obesity effects. The main objective of the study was to test if some of the effects on leanness and obesity resistance of the L-line Fob3b2 could also be due to the effect of this QTL on nutrient digestibility and bioavailability. The F2 littermates carrying either the Fat line segment within the Fob3b2 region or the L-line were compared when fed the high-fat diet. Eleven mice per genotype were individually housed in metabolic cages. In 5-day experimental period, body mass and diet intake were measured. The part of study was done on the F and L line and tested the difference in apparent protein digestibility on low-fat (LFD) and high-fat (HFD) diet. The nitrogen content was determined in the diet, faeces, and urine based on which, the apparent protein digestibility, apparent protein biological value and apparent net protein utilization were calculated There were no significant differences in any of these parameters on congenic line, confirming that the phenotypic effect on adiposity between the genotypes in the M2-F2 population was not due to the differential effect of the Fob3b2 locus carrying the Tst gene on protein utilization. We conclude that the observed phenotypic effects of this gene region are due to direct metabolic actions rather than the effects on nutrient absorption and nitrogen utilization since there were no differences in apparent protein digestibility between L and F lines, irrespective to HFD or LFD. The age of animals had significant effect on the level of digestibility.

The article provides information on the development of new functional snacks based on local traditional and non-traditional raw materials. The uniqueness of the composition of snacks is associated with the presence in the recipe of dry mare's and camel's milk, as well as fruit and vegetable purees, dry strains of lacto and bifidobacteria, fucoidan and other food ingredients with increased nutritional and biological value. Evaluation of the chemical composition of the nutritional and biological value of snacks indicates their targeted antioxidant, detoxifying and immunostimulating properties. Functional snacks are recommended for use by the general public, including climbers, pilots of civil and military aviation, athletes, as well as in emergencies and conditions that adversely affect human health.

By-products are the potential source of animal protein obtained from brood chickens and egg-laying hens. Certain by-products like gizzards and combs are quite tough and possess low nutritional and biological value due to their high content of connective tissue. Biotechnological processing improves the quality parameters of collagen-containing by-products. In this article a probiotic starter culture of propionic acid bacteria, which have high proteolytic activity, was used to treat the gizzards and combs of brood chickens. Before processing of by-products with starter culture, physical and chemical parameters and the yield of by-products in relation to poultry live weight were analyzed and recorded. 5%, 10% and 15% starter culture were added to the tested samples of chopped by-products, the samples were kept at a temperature of 30 °C, and every 4 hours the following functional and technological parameters were monitored: moisture binding capacity, water holding capacity (MBC and WHC) and yield of the product after heat treatment. The results proved that increase of starter culture amount and longer exposure of by-products to hydrolysis led to decrease of functional and technological parameters values, but for the combs those parameters remained at a sufficiently high level compared to the gizzards, as the gizzards were exposed to more intense hydrolysis than combs. The decrease in the pH value correlated with the dynamics of MBC and WHC changes; and dynamics of the product yield after the heat treatment. Also the stained histological preparations were studied in order to assess the influence of biotechnological processing on by-products microstructure, where significant differences were found in the morphological structure of muscle and collagen fibers of hydrolysates of combs and gizzards exposed to action of bacterial concentrate. The results of rheological studies showed that hydrolyzed chicken combs differed from gizzards; the combs were denser and featured more elastic structure due to a lower degree of hydrolysis by bacterial enzymes. In general, the properties of collagen-containing by-products (muscular gizzards and combs) change significantly after being exposed to enzymes of propionic acid bacteria.

The paper is devoted to solving the problem of the nutritional and biological value of rye-wheat bread by enriching it with non-traditional local plants raw materials – linseed flour and rice husk fiber. Rice husks are rarely used in bakery production, and in most cases remain unprocessed. However, this research defined the right ways for using them and set as a preliminary work in this field. The study has been carried out in two stages: firstly, linseed flour was added to the rye-wheat bread recipe in an amount of 5; 10; 15; 20% to the weight of wheat flour. Secondly, dietary fiber was added to these experimental samples, prepared from rice husks without removing amorphous silicon dioxide in an amount of 0.3; 0.5; 0.7% to the total mass of rye-wheat flour. The optimization of the ratios of the flour components with a simplex-lattice design was carried out and the rheological measurements of dough and bread were conducted on the farinograph and Chopin alveograph. The study results experimentally found that mixtures of rye-wheat flour and linseed flour with the addition of fiber as “medium in strength” give bread with sufficient volume. The recipe optimization parameters indicated that rational percentage of fiber and linseed flour up to 0.5% and 15%, respectively allows increasing the nutritional and biological value of finished products, improves the crumb structure, gas-holding and water-holding capacity of bread, which in turn prevents the stale process and thereby increases the shelf life of finished products. The obtained results allow us to suggest that, this recipe optimization model could be used in further research, as studies in this direction are limited

Adding oat products to composite mixtures during baking increases the biological value of a new product. The purpose of the current study was to assess the biochemical properties of grain of the naked oat varieties ‘Baget’ and ‘Bekas’, to establish the rheological parameters of dough mixtures obtained by mixing white oat flour and whole grain flour with high-quality wheat flour according to the farinographic analysis. The introduction of oat products into the mixture affects the structural and mechanical indicators of the dough and can improve or worsen the quality indicators of bakery products. It is the decoding of the diagrams (Brabender farinograph, Chopin alveograph, etc.) that satisfy the questions related to the study of dough, with the characteristics of the physical properties of dough. There has been established a high nutritional grain value of the naked oat varieties (whole grain and white flour), when the protein percentage was on 5.3–7.6% more than in wheat flour and oil content was almost 6–5 times higher, which indicated great opportunities for using this grain crop in bakery. The study of the rheological parameters of dough showed that in composite mixtures the type of ingredients (whole grain flour, white flour) and the proportion of naked oats products (10, 20, 30%) differently affect the physical properties of dough, i.e. in relation to premium wheat flour, dilution of dough increases on 40–140 u.f., resistance of dough to extension (stability) reduces on 2.5–6.0 min and valorigraphic number reduces on 10–36 u.v. When mixing with white oat flour compared to whole grain, the physical properties of the dough are characterized by the best indicators, however, the introduction of whole-ground oat flour in the mixture retains all the benefits of oat grain. There were no significant differences between the naked oat varieties ‘Baget’ and ‘Bekas’ in the effect on the rheological parameters of the dough. When using oat flour in mixtures, there was identified a tendency to deterioration of the physical properties of dough, the differences were significant in terms of the resistance of dough to extension (stability) and valorigraphic number.

Taking into account modern requirements, the biological value of the protein component of the Simmental breed Krov milk produced under intensive technology has been studied. The amino acid composition of milk proteins was identified by high-performance liquid chromatography. To analyze the biological value of proteins, calculated indicators were used: the amino acid index and the amino acid number (skor) of essential amino acids, taking into account the true digestibility of each of them. As a result of the research, all the essential and interchangeable amino acids and a fairly high concentration of many of them were identified. Among the essential amino acids, the largest amount in the milk samples contained phenylalanine+tyrosine, leucine, lysine, valine, and the smallest-tryptophan, methionine+cysteine. In the aggregate of interchangeable amino acids, the highest saturation was noted for glutamic acid+glutamine, arginine, proline, and the lowest-glycine, alanine. The predominance of interchangeable amino acids was noted in the protein component, which caused a low amino acid index (0.61). The amount of assimilated essential amino acids was higher relative to the reference protein. However, the amino acid score had a wide oscillation amplitude: from 149.6 % for the amino acids phenylalanine + tyrosine to 61.2 and 87.8 %, respectively, for tryptophan and methionine+cysteine, which indicated their imbalance. The essential amino acids tryptophan and methionine+cysteine were limiting, since their amino acid number (skor) is below 100 %.

It has been established, that the development and implementation of the practice of using biologically active components of nutrition is one of the areas of modern dietetics, which are developing dynamically. Apiproducts are a very promising source of biologically active substances. Fundamental studies of domestic and foreign scientists have proven their high biological value, versatile pharmacological activity (anti-inflammatory, antimicrobial, analgesic, antioxidant, immunostimulatory, anti-radiation, etc.). The use of apiproducts for the prevention and treatment of a number of human diseases is a comprehensively studied phenomenon in terms of beekeeping, botany, chemistry, microbiology, pharmacology, medicine and pharmacy. It has been proved, that the action of individual apiproducts and their components allows to use them effectively in practical apitherapy. Because these products differ significantly depending on their chemical composition, they are divided into two groups. The first includes honey, flower pollen, perga (a product of conservation of flower pollen in a hive) and, in part, drone brood. As mixtures of valuable BAS and metabolites, they are used mainly to increase the biological value of traditional foods and create new compositions. The second group includes bee venom, propolis and, in part, royal jelly, which are therapeutic agents by their nature and are used to correct physiological functions and metabolism, have regulatory effects, and their action is realized through the effect on the lipid matrix of biological membranes and membrane proteins. It has been established, that the use of these food components in health nutrition and production of health and medical foods is a promising area

The most important phytonutrients of sea buckthorn ( Hippophae rhamnoides L.) berry are carotenoids, flavonoids, anthocyanins, sugars, organic and amino acids, water and fat-soluble vitamins, tannins and pectin substances, phospholipids, macro- and microelements. In the Chelyabinsk region it is almost impossible to find well-groomed industrial plantings of sea buckthorn, since its main areas (600 hectares) are concentrated in amateur and home gardens. To form a healthy diet, it is important not only to ensure the safety of horticultural products, but also to raise the priority of signs that determine consumer demand, namely the quality and chemical composition of fruits, their saturation with the necessary micronutrients. While studying food and biologically active substances of sea buckthorn berries of the "Velikan" variety growing in different garden agrocenoses of the Chelyabinsk region it has been determined that sea buckthorn berries cultivated in the garden "Lokomotiv-1" (Chelyabinsk) contain more flavonoids (by 21.4 %), proteins (by 17.4 %), mineral elements - Al (in 5.3 times), V (3.4 times), Cu (3 times), Ti (2.4 times), Ca and Fe (1.9 times), Mn (1.5 times), in berries obtained in the garden "Dizelist-1" (Chelyabinsk region, Troitsk) contains more sugars (by 15.1 %), as well as Mo and Sr (2-2.3 times), Se (1.4 times), Cr (by 76.2 %), etc. In terms of Se content, the studied fruits exceed the clarke values by 4-6 times; in terms of Pb content, they turned out to be as close as possible to the upper limit of MPC according to the requirements of the Technical Regulations of the Customs Union. The study of the mineral composition of sea buckthorn is especially important in the context of the ecological disadvantage of the Chelyabinsk region due to the pollution of the environment with heavy metals.

Polyodon spathula is a valuable species of sturgeon native to North America that has acclimatized very well in Europe. Detailed knowledge of the quantitative and qualitative productive performance of paddlefish meat is of interest. Through this article, we aimed to highlight the chemical composition, cholesterol, and collagen content of fillets issued from paddlefish aged two and three summers and to highlight, as well as the nutritional value, the profile of fatty acids and amino acids, the sanogenic indices and the biological value of proteins for the epaxial and hypaxial muscle groups. The chemical analysis of the fillets by age indicated slightly higher values in summer three, compared to summer two: +5.32% dry matter, +0.89% protein, +41.21% fat, therefore +10.94% gross energy and for collagen by 2.94%; instead, for water, minerals and the W/P ratio the values were lower by 1.52%, 10.08%, and 2.29%. The nutritional assessment revealed that paddlefish has a meat with high PUFA content (approx. 22% of total fatty acids) and good values of sanogenic indices (Polyunsaturation Index = 7.01–8.77; Atherogenic Index = 0.57; Thrombogenic Index = 0.38–0.39; Hypocholesterolemic Fatty Acids = 33.01–41.34; Hypocholesteromic/Hypercolesteromic Fatty Acids ratio = 1.9). Also, the proteins of these fish are of good quality for young and adult consumers (EAA index = 156.11; Biological Value = 158.46; Nutritional Index (%) = 28.30) and good enough for children (Essential Amino Acids Index = 96.41; Biological Value = 93.39; Nutritional Index (%) = 17.45).

The rapid development of the latest innovative technologies that improve human life have tragic consequences. The irreversible and cruel reverse side of the progress is the emergence of various diseases and pathologies. Cardiovascular diseases occupy a leading position in the morbidity and mortality of people of different ages in the world. Functional food products can be used to prevent diseases of the heart, the blood vessels and the thyroid gland. One of the ways to increase immunity of the population can be the expansion of specialized food production that are balanced in the content of macro- and microelements, take into account the peculiarities of metabolic processes, pathological conditions that occur at different ages in people. Pork, enriched with microelements in its lifetime, is a promising raw material for improving the technology of organic pork production. The developed technology for obtaining organic meat raw materials has passed various tests in the farms of the Krasnodar Territory, which are the main suppliers of pork for the manufacture of specialized functional products. For the manufacture of a specialized meat product, meat without lard has been used. The article presents studies of nutritional and biological value, nutritional adequacy of pork and by-products enriched while alive. Specialized pasteurized meat products enriched with microelements (iodine and selenium) have been developed, and the qualitative composition of a specialized meat product has been disclosed, which can be used to prevent cardiovascular diseases and diseases of the thyroid gland. Experiments have been carried out on laboratory animals (white rats) to confirm the biological effectiveness of a specialized meat product.

The article presents the results of studies to identify the influence of abiotic factors on the grain quality of varieties and lines of winter triticale of the Samara Research Institute of Agricultural Sciences for 2017-2020, in comparison with varieties of winter rye and wheat. The studied crops were cultivated in the fields of the institute, located in the black earth steppe of the Middle Volga region. Technological properties were determined in the laboratory of the technical and analytical service of the Samara NIISH and at the Faculty of Technology of the Samara State Agrarian University according to standard generally accepted methods. The data obtained indicate a higher productivity of modern triticale varieties, compared to rye and wheat. In terms of grain quality, these varieties and lines are superior to winter rye varieties and are not inferior to wheat. On average, over 4 years, the content of HAC in triticale varieties was (1.09-1.51 mp · S), with a variation by year, depending on climatic conditions from 0.90-1.53 mp · S in 2017 to 1.01-1.59 mp · S in 2018. It was revealed that the varieties Krokha and Arcturus were distinguished by the fat content of 1.81-1.85 %, the variety Spika and liniya 9385-4/14 1,72-1,75% – by ash content. In terms of the content of almost all amino acids, the grain of the 830-4/13 line was superior to the rest of the studied samples. The amount of protein in the grain varied from 11.7 to 15.6% over the years, and fat from 0.61 to 2.55%. A high yield relationship was observed with the content of pentosans r=-0.82±0.08, arginine r=-0.72±0.12, with the mass of 1000 grains r=0.67±0.14, histizine r=0.61±0.16, leucine r=-0.69±0.13, sodium content r=0.86±0.06, and depended on the amount of precipitation in April r=0.81±0.08. The necessity of analyzing the amino acid composition of triticale grain to determine its biological value is justified

Expanding the range of products of therapeutic and prophylactic orientation is a priority direction of the state policy in the field of healthy nutrition. Products enriched with functional components improve the general state of the body, have high organoleptic characteristics in combination with increased biological value. The production of synbiotic ice cream is one of the promising trends in the dairy industry. Due to its unique composition and technological peculiarities, the ice cream retains the desired organoleptic and functional properties. Pro- and prebiotic components in it are conserved by freezing, and activated only when they enter the organism. It has been established experimentally, that high concentrations of monosaccharides have an inhibitory effect on the development of probiotic cultures, therefore, the purpose of the research is to simulate the fermentation process of the ice cream enriched with prebiotic components, in order to determine optimal characteristics and establish rational syrup concentrations. Lactic bacteria of Lactobacillus acidophilus species that remain active in a wide temperature range and suppressing pathogenic microflora, as well as berry syrup with a high concentration of vitamins and minerals have been used as components that ensure the functionality of the product. Phased addition of syrup has been recommended for the ex- periment. As a result of the research, a model recipe for synbiotic ice cream has been developed, the quantitative regularities of the influence of various concentrations of fructose syrup on the process of fermentation of compound have been determined, organoleptic characteristics of soft-serve ice cream have been investigated, a power-law model of the dependence of the duration of fermentation on the mass fraction of fructose syrup has been obtained. It has been found that a decrease in the initial concentration makes it possible to shorten the technological cycle and regulate the acidity index.

A deficit in protein intake can severely halt children growth, and is associated with both structural and functional pathologies of the brain, as well as increase the risk of contracting infectious diseases. Protein-enriched defatted meals from sacha inchi (Plukenetia volubilis) (sdSI) and chocho (Lupinus mutabilis) (sdCH) were obtained by a solvent defatting procedure. The concentration of protein was higher in sdSI (64%) than in sdCH (60%). Lysine and tryptophan were the limiting amino acid (AA) in each case. However, the AA profile of the protein meals met daily intake FAO requirements for adults, while their blends met the requirements for infants. The sdSI had an in vitro digestibility of 100. Regarding functional properties, sdSI presented higher values for water absorption index (3.5), emulsifying activity (56%), and foaming capacity (167%), while the sdCH had a slightly better oil absorption capacity (2.8). However, when mixing sdSI with sdCH some improvements in functional properties were observed. The nutritional and functional properties of the mixtures of meals can be used to develop protein-enriched foods and beverages.

Enzymatic hydrolysates of mechanically deboned meat (MDM) for a long time have been used as flavouring and functional food ingredients in the food industry and also as the bases of formula foods for special dietary uses.The aim of the present study was to produce MDM hypo-antigenic products with improved digestibility and high biological value to be used as a milk protein alternative. turkey MDM was treated with digestive enzymes (trypsin and/or α-chymotrypsin, or pancreatin), followed by freeze drying. The optimised reaction conditions of hydrolysis were at 6% (w/v) of meat protein in 0.1% NaHCO3 buffer, pH 7.5; pancreatin enzyme with 50 TAME units/g meat protein substrate, 37 °C and 60 min). Hydrolysates (MDMH) were assessed for degree of hydrolyses (DH, %) by using trinitrobenzenesulphonic acid method and MW distribution by SDS-PAGE. Modification of immune reactive binding sites in MDMHs was monitored by immunoblot with cow’s milk, chicken egg or meat allergic human patients’ sera. Biological value indices (True Digestibility (TD), Net Protein Utilisation (NPU), Biological Value (BV)) were determined using rat feeding trials. Among the MDMH products, the pancreatic hydrolysate proved to be the most favourable in terms of biological value and digestibility as well as hypoallergenic property.

The technology for obtaining biologically active substances of a lipoid nature, enriched with omega-3 acids, from waste from the processing of hydrobionts by means of electrochemical hydrolysis and cryoconcentration has been developed. A comparative analysis of the composition of wastes from cutting herring and trout is carried out, and the expediency of their use for obtaining biologically active substances of a lipoid nature is shown. A technological scheme has been developed and fat yields have been determined when it is obtained from fish waste by an electrochemical method. The fatty acid composition of the fat obtained by the electrochemical method has been determined. It was found that cryoconcentrated fat obtained from wastes from trout and herring cutting by the electrochemical method has a significantly increased content of omega-3 acids and, accordingly, biological value compared to edible and medical fish oil from the liver of the cod family. It was found that during cryoconcentration, the concentration of polyunsaturated fatty acids increases, reaching values close to 90%, which allows the resulting product to be classified as biologically active additives. It was shown by calculation that to create functional fish-based food products from fish of the salmon family, it is sufficient to introduce 4 g of the obtained biologically active additive per 100 g of the product. There is also an improvement in the organoleptic properties of foods from lean fish species. It has been shown that in order to meet 30% of the recommended daily intake of omega-3 acids in the development of functional food products based on rainbow trout and Atlantic herring, it is necessary to introduce 1.98 g and 1.8 g of cryoconcentrated fish oil. After encapsulation in nanocapsules, the drug will be suitable for enrichment with omega-3 acids in any food products, which is the subject of further research.

Application conditions of the ACTIVA® TG-TI transglutaminase combined with setting agents (gelatine and chitosans of various modifications) are substantiated for moulded food processing from waterlogged fish raw materials targeted by deep-water fishery, i.e. giant grenadier (Albatrossia pectoralis). It was shown that combining different setting agents ensures time reduction of gel formation with a minor change in their development and melting temperatures. Adding an enzyme preparation leads to the formation of thermostable gels with a 1.5-fold increased strength. When forming gels from the grenadier muscle tissue in the presence of setting agents of different concentrations, the quality of soluble muscle proteins reduces, most prominently with gelatine and chitosan ascorbate. Physicochemical parameters of the structured gels prepared from the muscle tissue of giant grenadier (moisture retention capacity, tensile strength, water activity) and their organoleptic profiles were measured. Based on the results, we have shown that adding 3% of gela-tine, 0.06% of high-molecular chitosan and 1% of transglutaminase may be employed for processing moulded fish products. The digestibility of the protein components in obtained samples did not depend on fermentation. The overall protein deposition was between 0.38 and 0.56% of the sample mass. The total biological value of samples ranged from 78 to 134% when studying their effect on the growth of T. pyriformis testing culture.

Chemical composition and nutritional and biological value of roe are investigated for autumn chum salmon from the Amur River sampled during their run for spawning from the estuary to the area of Khabarovsk city. Color and strength of roe were examined, content of proteins, lipids and minerals was measured, amino acid and fatty acid composition was determined. Caloric capacity and biological value of the samples were usual for this species. Physical properties of the roe (strength, color intensity) practically did not change in dependence on distance from the river mouth. Stable quality of roe in the run of spawning migration in the lower Amur is concluded.

The overview presents the literature data and the results of our own research on prospects of using the chicken eggs as the basis of functional foods. The composition of chicken eggs and their components, characteristics of egg white proteins properties are presented thereto. The biologically active compounds included into egg composition are analyzed. The data on the biological value of egg white are given. The characteristic of egg white foaming ability is presented. It has been shown that the ability of proteins to form stable intermolecular structures, especially with partially denaturated proteins, allows them forming viscoelastic superficial films that ensure foam stability. The high foaming ability of chicken egg protein macromolecules is directly related to their interphase properties, i. e. the ability to form interphase layers at the “liquid — gas” interface. The foaming properties of the various egg proteins are not equal, and therefore they contribute to foaming properties at various extents. The model of egg white proteins gelation is considered and the factors influencing the gelation process are described. It has been shown that very important changes in proteins properties are caused by denaturation. The proteins lose their ability to hydrate; the protective aqueous shell around the globules disappears, the proteins stick together, grow larger and lose solubility. This process is called coagulation. The influence of denaturation and aggregation on variations of protein properties is described below. Data on protein fortification with functional ingredients (calcium, iodine, plant polyphenols) and creation of functional egg and meat foods are presented here.

The topicality. To date, the structure of the population’s diet has significant deviations from the formula of a balanced diet in terms of protein intake, including sulfur-containing, which causes the formation of risk factors for the development alimentary and alimentary-dependent diseases. For the prevention of diseases caused by protein deficiency, it is promising to increase its content in vegetable dishes due to the integrated use of raw materials and dietary supplements of high biological value. It is advisable to use food combinatorics in the innovative technologies development for vegetable dishes of improved amino acid composition. The purpose of the study is food combinatorics of amino acid composition of potato croquettes with soy flour, wheat germ; natural shrimp powder “Rieber Food Ingredients” and lentil-spirulina filling in terms of content and balance of essential and substitute amino acids and the degree of their assimilation by the human body. Research methods. Physicochemical, mathematical and statistical methods of experimental data processing with the use of information technologies, methods of ion exchange liquid column chromatography and qualimetric methods have been used. Results. Research is aimed at the use of food combinatorics and scientific substantiation of the amino acid composition of potato croquettes with the use of protein vegetable and non-fish water raw materials and dietary supplements. As a result of scientific researches the food combinatorics at development of technology of vegetable dishes has been carried out, the amino acid structure has been proved and experimentally generalized, the amino acid score and balance of sulfur-containing amino acids in the complex use of potato mass with wheat germ, soy flour, natural shrimp powder “Rieber Food Ingredients” and lentil-spirulina filling in culinary products have been analyzed. The social effect of fuller use of vegetable, non-fish water raw materials, expansion of the range of vegetable dishes with improved amino acid composition and consumer properties of potato dishes in restaurants, preservation and protection of public health has been confirmed. Conclusions and discussions. Food combinatorics was carried out during the potato croquet technology development with the use of protein vegetable and non-fish water raw materials, which contributed to the improvement of the amino acid composition of vegetable dishes and, in particular, to the increase of their biological value.

The scientific review article allows to evaluate the relevance of scientific research of gluten-free bakery and confectionery products produced on the basis of secondary types of flour and flour mixtures, modern sources of non-traditional types of fruit and berry raw materials, innovative technologies, functional ingredients, designed to maintain the health of the population suffering from gluten intolerance. Much attention is paid to the use of amaranth seed flour in bread production, which has a higher biological value compared to traditional types of flour. Adding, along with amaranth flour, chufa tuber flour, carrot, rowan, pumpkin, apple and other powders to the dough can expand the diet of patients with symptoms of gluten intolerance. In particular, the apple powder contribute to the prevention of cardiovascular disease, excess weight, removal of heavy metals from the body, prevent the development of atrophy of the skeletal muscles, and carrot powder - speed recovery from a nervous breakdown, prolonged stress, strengthen the heart muscle, improve blood vessels, enhance immunity, prevent the development of atherosclerosis, hypertension, etc. The technology of production of gluten-free products based on amaranth seed flour mixed with corn or rice flour continues to be improved, which significantly increases the nutritional value of these products and expands the range of affordable gluten-free food products of domestic production. Another example is croissants baked on the basis of rice, buckwheat, flaxseed, almond and Teff flour. The analysis of organoleptic and physicochemical parameters showed that they meet the requirements of quality and high taste and aroma characteristics. Gluten-free bread based on chia flour, buckwheat flour, flax, soybean, amaranth, millet, tapioca, sorghum, peas, quinoa, rice and lupine flours contain a high amount of dietary fiber, positively influencing the quantitative and qualitative composition of intestinal microflora and helping to preserve the freshness of baked products. Despite the commonality of existing approaches in the production of gluten-free products, scientists suggest supplementing the existing recipes with secondary types of flour, fruit and berry and other functional components. Thus, the problem of using gluten-free flours in the production of functional products remains the object of increased scientific attention on the part of scientists, manufacturers and consumers.

The demand for natural products is constantly growing in the current environmental situation. This encourages manufacturers to increase the production of these goods. However, almost all branches of the food industry use nowadays a large number of biologically active additives, mainly of synthetic origin. In this regard, the introduction of natural vegetable supplements in the composition of a traditional food product will contribute not only to an increase in the nutritional and biological value of the product, but also to expand the range of healthy food products. The possibility of using dietary fiber from root crops of scorzonera, immobilized on biopolymers, and chickpea flour obtained from chickpea beans bioactivated by germination in the amount of 5% and 15%, respectively, was studied in the work. Scientific substantiation of the effectiveness of the use of vegetable filler in the production of combined soft cheese was given. The cheese product produced has a mass fraction of fat in dry matter of 4.5%, a mass fraction of moisture not more than 64%, a mass fraction of salt not more than 2.0%. The use of dietary fiber as a food additive in cheese production gives the product a functional value. Dietary fiber, introduced in an amount of 5.0%, increases the yield of the finished product by 11-11.5%. Thus, applying the proposed technology for the production of a soft cheese product, it becomes possible to solve a number of technological, environmental and economic problems, including an increase in the nutritional value of a soft cheese product, due to enrichment with dietary fiber, macro- and micronutrients, as well as an increase in the biological value of the developed product by balancing the amino acid composition

One of the most important problems of the population's nutrition is the high caloric content of flour confectionery products, due to their oversaturation with carbohydrate and fat components. This trend leads to micronutrient deficiencies, and later to metabolic problems and deteriorating health. In recent years, there has been an expansion of the range of waffles, improving technology through the wider introduction of various ingredients of plant origin. Crustilian is a crunchy product or component of a dish that has a crispy texture (waffles, cereal breads) or a surface (a crust of bread or potato balls with a fried crust). Crustilian is also used as a layer for cakes or desserts, which usually consists of waffle crumbs, chopped nuts and melted dark chocolate. There are still many variations for making crustilians. The range of applications of crustilian in the manufacture of sweet dishes is extremely wide. The nutritional value of crustilians is determined by the composition of raw materials and can be significantly increased due to the rational selection of components, the introduction of enrichments in the recipe, as well as technological processing, which increases their digestibility. Improving the technology of crucifers waffles by increasing their nutrient density will expand the range of products with functional ingredients, and enrichment of the micronutrient composition due to valuable plant raw materials will improve the nutritional value and organoleptic characteristics of the finished product. A line of crustilians with a high content of biologically active compounds has been developed due to the introduction of superfoods such as flax seeds, sesame seeds, sunflower and walnuts and peanuts into the product formulation. It is established that the use of peanuts and walnuts, in the composition, makes the dough less crispy and more fatty. The presence of flax and sesame does not reduce the crunchiness of the finished product, which is characteristic of crustilians, but, on the contrary, slightly increases this figure. It is proved that the index of nutritional density of crustilian made according to the developed recipe in comparison with the prototype is characterized by an increase in the index of nutritional density in three times, while the mass fraction of the macronutrients increases from 1.26 to 2.5 times, depending on the micronutrient. increases only 1.1 times.

Darbe aprašyti funkcionalieji kepinio komponentai, pasižymintys sveikatinančiomis savybėmis, gerinantys gaminio maistinę ir biologinę vertę. Išnagrinėta VšĮ „Valgykla“ veikla, produkcijos asortimentas, įrenginiai, RVASVT sistema. Darbe parengtas naujo gaminio aprašas, nustatyti rizikos veiksniai, ir svarbūs valdymo taškai, apskaičiuotas gaminio maistingumas, atlikta juslinė analizė. VšĮ „Valgykla“ asortimente pasigesta gaminių, kurie būtų tinkami veganams bei sveiką mitybą propaguojantiems, kad būtų be pridėtinio cukraus. Todėl atsižvelgus į tai, priimtas sprendimas, gaminti būtent toks gaminį., The thesis describes the functional components of the bakery products with healing properties that improve both the nutritional and biological value of the product. The activities of Valgykla Public Institution, product selection, equipment, and HACCP system have been discussed. Also, the thesis contains a description of a new product, identified risk factors and important check points, as well as calculated nutritional value of the product and sensory analysis. The selection of Valgykla Public Institution lacks the products for vegans and healthy diet promoters that would contain no added sugar. Hence, in view of this, it has been decided to make such a product.

The unresolved problem of quail is feed stress and high sensitivity of poultry of this species to feed quality. To eliminate such consequences it is possible with the use of various biologically active feed additives. However, their introduction into the diet automatically raises questions about the quality and safety of the products for the consumer. The purpose of the research is to conduct a veterinary and sanitary assessment of the quality of quail meat for feeding NDC. The purpose of the research is to conduct a veterinary and sanitary assessment of the quality of quail meat for feeding NDC. The object of study were the quails of the Pharaoh breed, at the age of 24 were divided into two groups: experimental and control 30 heads each. The birds were kept in cages with free access to food and water. Quails of the experimental group were added to drinking water feed additive "Nanocerium" at a dose of 8.6 mg per liter of drinking water. Nanocerium feed additive is an aqueous dispersion of NDC with average nanoparticle sizes of 2–7 nm. Antemortem examination of quails of both groups revealed a satisfactory clinical condition of birds. The average weight of the gut carcass of quails of the experimental group was higher by 8.3% compared to the control. Examination of 20 carcasses of quails showed that they can be classified as first class. According to organoleptic parameters, quail meat is of good quality in veterinary and sanitary terms. Microbiological studies of quail meat indicate no effect of NDC in the applied doses on its bacterial contamination. Chemical parameters of meat (pH, aminoammonia nitrogen, volatile fatty acids during storage in the refrigerator) (5 days, t = 4–5 ° C) tended to increase and were within the normal range for fresh product. Tissue in quails of both groups was not detected. The biological value of quail meat in both groups was identical and no toxicity. Tasting evaluation of the broth and meat showed that NDC did not affect the studied taste. The obtained results show that the quails that received the feed additive "Nanocerium" with water according to the indicators of veterinary and sanitary examination are of good quality, which allows to use it in human food without restrictions. Key words: poultry, nanocerium, organoleptic evaluation, microbial contamination, biological value, tasting evaluation.

Борошняні кондитерські вироби традиційно є одними зі складників у раціоні харчування населення багатьох країн світу, тому розширення асортименту цих виробів із використанням біо- логічно цінної сировини спеціального спрямування є актуальним. Для обґрунтування рецептурного складу безглютенових бісквітних напівфабрикатів було замінено пшеничне борошно вищого ґатунку на борошно кукурудзяне і кунжутний шрот у різних співвідношеннях: 50:50; 70:30; 30:70. На основі проведених досліджень органолептичних і фізико-хімічних показників якості безглютенових бісквіт- них напівфабрикатів встановлена можливість використання кукурудзяного борошна і кунжутного шроту на заміну пшеничного борошна у співвідношенні 50:50. Розроблені вироби характеризуються відмінними органолептичними показниками, добрими об’ємом та пористістю. Встановлено, що в розроблених бісквітних напівфабрикатах з’являється приємний кунжутний смак, а в кольорі – наси- чені жовті відтінки. Розроблені бісквітні напівфабрикати характеризуються збільшеним вмістом білків, мінеральних речовин (калію, кальцію, магнію, фосфору та заліза), вітамінів, а також харчових волокон. Це пояснюється тим, що використана нетрадиційна сировина переважає пшеничне борошно за вмістом цих речовин. З огляду на більш збалансований амінокислотний склад кунжутного шроту і кукурудзяного борошна біологічна цінність розроблених напівфабрикатів також збільшується. У результаті проведених досліджень обґрунтовано доцільність використання кукурудзяного борошна і кунжутного шроту для розробки рецептури бісквітних напівфабрикатів. Виявлено закономірності, які визначають позитивний вплив на органолептичні і фізико-хімічні показники кукурудзяного борошна і кунжутного шроту в співвідношенні 50:50. За харчовою та біологічною цінністю найкращим був зразок бісквітного напівфабрикату із заміною пшеничного борошна на 30% кукурудзяного борошна і 70% кунжутного шроту, але цей зразок за органолептичними показниками значно поступався іншим. Використання випеченого бісквітного напівфабрикату на основі кукурудзяного борошна і кунжут- ного шроту в співвідношенні 50:50 дасть змогу розширити асортимент безглютенових кондитер- ських виробів. Заміна пшеничного борошна на кукурудзяне борошно та кунжутний шрот дала змогу повністю вилучити з рецептури бісквіту глютен, що й було основною метою наших досліджень.

The deficit of feed-grade protein for the productive animals and poultry is presently propelling the interest toward the use of the hydrolysates of slaughter wastes as the potential protein sources. The slaughter wastes of poultry (feathers, intestines, blood, heads, shanks, meat-bone residues of the deboning) can mount up to one third of the initial bodyweight. Earlier we have developed the two-stage technology of easily digestible protein additives involving the hydrolysis and fermentation of protein from poultry slaughter wastes (primarily keratin from feathers and collagen from meat-bone residue). The study presented is a pioneer evidence that the substitution of these additives for fishmeal in diets for broilers provides more rapid growth and does not deteriorate the protein, fatty and amino acid profiles, concentrations of non-peptide, peptide and residual nitrogen, technological properties of meat. The study was performed in 2019 and aimed at the comparative evaluation of meat quality at 38 and 49 days of age in floor-housed broilers fed standard diet and three experimental diets with these hydrolysates. The broilers (cross Ross-308, 35 birds per treatment) kept in conditions of the Center for Genetics & Selection “EPH Zagorskoye” (Moscow Province) were allotted to four treatments partially slaughtered at 38 days of age and partially at 49 days. Control treatment 1 was fed a standard broiler diet with fishmeal; in experimental treatments fishmeal was substituted by enzymatic hydrolysate of feathers (treatment 2), enzymatic hydrolysate of collagen (treatment 3), mixture of these two hydrolysates with addition of probiotic Bacell M (0.2 %), and bran (0.5 %) as a source of vegetable fiber (treatment 4). It was found that supplementation of diets with the hydrolysates of keratin and collagen significantly improved live bodyweight in broilers by 8.78-10.89 % in compare to control (p £ 0.001). The content of peptide nitrogen in breast muscles in treatments 1 and 4 tended to grow with the increase in slaughter age (in treatment 1 from 0.07 at 38 days to 0.27 % at 49 days; in treatment 4 from 0.07 to 0.35 %). The number of protein fractions (with molecular weights from 100 to < 20 KDa) in meat increased with slaughter age with all studied diets. Protein of breast muscles contained more isoleucine, leucine, valine, and phenylalanine at 38 and 49 days of age in compare to thigh meat with all studied diets. The digestibility of essential amino acids in breast muscles in treatment 4 grew from 81.78 % with slaughter at 38 days to 90.29 % at 49 days. Evaluation of biological value of meat revealed better balanced ratios of the essential amino acids in breast muscles in control treatment at 38 days of age (the difference in the amino acid score 70.53 %) and in treatment 4 at 49 days of age (the difference in the amino acid score 66.48 %). In thigh meat this difference was higher in treatment 3 at 38 days of age (59.69 %) and in treatment 4 at 49 days of age (61.43 %). There were no significant differences between the treatments in concentrations of fatty acids in meat. The conclusion was made that the use of new protein additives based on the enzymatic hydrolysates of feathers and collagen in diets for broilers does not deteriorate the parameters of meat quality.

The deficit of feed-grade protein for the productive animals and poultry is presently propelling the interest toward the use of the hydrolysates of slaughter wastes as the potential protein sources. The slaughter wastes of poultry (feathers, intestines, blood, heads, shanks, meat-bone residues of the deboning) can mount up to one third of the initial bodyweight. Earlier we have developed the two-stage technology of easily digestible protein additives involving the hydrolysis and fermentation of protein from poultry slaughter wastes (primarily keratin from feathers and collagen from meat-bone residue). The study presented is a pioneer evidence that the substitution of these additives for fishmeal in diets for broilers provides more rapid growth and does not deteriorate the protein, fatty and amino acid profiles, concentrations of non-peptide, peptide and residual nitrogen, technological properties of meat. The study was performed in 2019 and aimed at the comparative evaluation of meat quality at 38 and 49 days of age in floor-housed broilers fed standard diet and three experimental diets with these hydrolysates. The broilers (cross Ross-308, 35 birds per treatment) kept in conditions of the Center for Genetics & Selection “EPH Zagorskoye” (Moscow Province) were allotted to four treatments partially slaughtered at 38 days of age and partially at 49 days. Control treatment 1 was fed a standard broiler diet with fishmeal; in experimental treatments fishmeal was substituted by enzymatic hydrolysate of feathers (treatment 2), enzymatic hydrolysate of collagen (treatment 3), mixture of these two hydrolysates with addition of probiotic Bacell M (0.2 %), and bran (0.5 %) as a source of vegetable fiber (treatment 4). It was found that supplementation of diets with the hydrolysates of keratin and collagen significantly improved live bodyweight in broilers by 8.78-10.89 % in compare to control (p £ 0.001). The content of peptide nitrogen in breast muscles in treatments 1 and 4 tended to grow with the increase in slaughter age (in treatment 1 from 0.07 at 38 days to 0.27 % at 49 days; in treatment 4 from 0.07 to 0.35 %). The number of protein fractions (with molecular weights from 100 to < 20 KDa) in meat increased with slaughter age with all studied diets. Protein of breast muscles contained more isoleucine, leucine, valine, and phenylalanine at 38 and 49 days of age in compare to thigh meat with all studied diets. The digestibility of essential amino acids in breast muscles in treatment 4 grew from 81.78 % with slaughter at 38 days to 90.29 % at 49 days. Evaluation of biological value of meat revealed better balanced ratios of the essential amino acids in breast muscles in control treatment at 38 days of age (the difference in the amino acid score 70.53 %) and in treatment 4 at 49 days of age (the difference in the amino acid score 66.48 %). In thigh meat this difference was higher in treatment 3 at 38 days of age (59.69 %) and in treatment 4 at 49 days of age (61.43 %). There were no significant differences between the treatments in concentrations of fatty acids in meat. The conclusion was made that the use of new protein additives based on the enzymatic hydrolysates of feathers and collagen in diets for broilers does not deteriorate the parameters of meat quality.

The paper emphasizes the importance of not only the quantitative but also qualitative composition of protein in nutrition. The authors propose protein classification into three main groups according to the concept of reference (ideal) protein. A mathematical model is examined to solve the task of rational mixture production upon the given profile of reference protein. Two variants of the criterion for formation of optimal composition are described. One of them presents the classical sum of squares of the residual for essential amino acid scores and 1. The second also presents the sum of squares of the residual for essential amino acid scores and 1 but with regard to only those amino acids, which scores are less than 1. The minima of these criteria at the set of variants for the content of ingredients are taken as targeted functions. The algorithm and the program of calculation were realized in the program environment Builder C++ 6.0. The macro flowchart of the algorithm is presented and detailed description of each block is given. The program interface before and after the start of the calculation module is shown. The main windows and interpretation of the presented data are described. An example of realization of the proposed mathematical apparatus when calculating a food model composition is given. Plant components (white kidney beans, flax, peanut, grit “Poltavskaya», dry red carrot) were used as an object of the research. Most plant proteins were incomplete. It is possible to regulate the chemical composition including correction of a protein profile by combination of plant raw materials. Analysis of alternative variants demonstrated that minimum essential amino acid score in the first composition was 0.79 (by the first criterion), in the second 1.0 (by the second criterion); the reference protein proportion in the mixture was 10.8 and 13.5, respectively, according to the first and second criterion. The comparative results by other quality indicators for protein in the mixture are also presented: the coefficient of amino acid score difference (CAASD), biological value (BV), coefficient of utility, essential amino acids index (IEAA).

Introduction. Sockeye salmon (Oncorhynchus nerka L.) is a valuable Pacific salmon. Sockeye heads are a significant share in processing sockeye salmon. Traditionally, fish by-products are used to make fishmeal. However, due to the high content of collagen proteins and fat in sockeye salmon heads, it is difficult to produce fishmeal from this raw material. Controlled enzymatic or combined hydrolysis allows protein, fat, and minerals to be extracted to supply the market with higher value products with desirable features. This research was aimed to analyse the chemical composition and biological value of hydrolysis products obtained from sockeye heads. Study objects and methods. We investigated hydrolysis products of sockeye salmon heads, namely protein hydrolysates, fat and sludge. Thermal hydrolysis and enzymatic-thermal hydrolysis were used for the tests. Thermal hydrolysis was realized in reactor. For enzymatic-thermal hydrolysis, the raw material was pre-treated by proteolytic enzyme Alcalase. The hydrolysates obtained were investigated. Chemical composition was determined in accordance with State Standard 7636-85. HPLC was used for molecular weight and amino acid analysis. Gas chromatography was used for fatty acid analysis. Biological value of proteins was determined by the balance of the amino acid composition comparing it with the “ideal protein model”. Results and discussion. Thermal hydrolysis resulted in the production of protein hydrolysate powder with protein content of 92.0% dry matter and a protein recovery rate of 39.6%. Combined hydrolysis resulted in the production of protein hydrolysate powder with protein content of 92.6% and a protein recovery rate of 83%. All protein hydrolysates contained all essential amino acids. The biological value of protein hydrolysate obtained by thermal and combined hydrolysis was 80.1 and 82.8%, respectively. Conclusion. Hydrolysed products obtained by thermal and enzymatic-thermal hydrolysis had a valuable chemical composition and could be recommended for food and feed use.

Wheat bread remains in the diet not only of Russians, but also of the population of other countries as a daily product, which makes it possible to consider it as an object for modifying the chemical composition and nutritional value in order to give it functional, dietary, or therapeutic and prophylactic properties. The purpose of the research is to study the composition of the functional components of non-traditional types of flour used in wheat bread technology in order to establish the effectiveness of their use. The objects of research were: bakery wheat flour of the highest grade, walnut flour, peanut flour, coconut flour. The analysis of organoleptic, physicochemical indicators and nutritional value of wheat bakery flour and various types of flour from walnut is carried out. An acceptable sensory compatibility of the studied raw material was established. Replacing a part of wheat flour in the recipe of bakery products with raw materials from peanuts or walnuts is a reasonable condition for eliminating the deficiency of dietary fiber in them. Increasing the biological value of products and the content of polyunsaturated fatty acids in it due to the use of peanut and coconut flour is of undeniable nutritional value. The source of phosphorus and copper can be considered flour from walnuts and peanuts; iron and selenium – walnut and coconut flour; magnesium and calcium – walnut flour. In a practical way, the effectiveness of replacing wheat flour in the recipe of bakery products with raw materials from nut-bearing raw materials has been established to increase the content of functional food ingredients in finished products.

Using nanotechnologies to obtain compounds of mineral substances is promising because of the importance of the amount of mineral substances, but the limiting factor is particularly their bioavailability in the digestive tract. The conducted studies determined the stimulating effect of nanoaquachelates of separate biogene microelements on the course of biochemical processes of the organisms of animals, their productivity and quality of the obtained products. However, the mechanisms of impact of carboxylates of separate elements and their combinations on functioning of physiological systems of various species of animals, level of transformation of these elements into the products and their biological value remain unstudied. Therefore, the objectives of the study were changes in hematological biochemical and productive parameters of the organisms of rabbits consuming I, Se, S, the impacts of these elements on their organism from the 53 to 85th days of life. Studies were performed on young Termonde rabbits. Animals of experimental groups I, II, III, and IV were fed with feeds of the diet of the control group for 24 h with water containing solution of І, Se, S calculated respectively as 2.5, 5.0, 10.0 and 20.0 µg of І/L of water. The experiment lasted 45 days, including a 12 day preparation period and 33 day experimental. On the 53rd day of life of animals in the preparation period, and 68th and 85th days of life in the experimental periods (15th and 33rd days of watering with supplements), we collected samples of blood from the marginal ear vein of 6 animals (3 males and 3 females) of the group for hematological and biochemical studies. We determined that watering with the solution of microelements І, Se, S led to changes in the overall amount of white blood cells, making it 17.1% lower in the blood of animals of the experimental group II, 26.2% higher in group ІІІ on the 15th day, and 12.0% higher in group IV on the 31st day of the experiment compared with the control. Absolute amounts of lymphocytes, monocytes and granulocytes by the periods of the study were observed to have no significant changes throughout the study, though their parameters were within the physiological values compared with the control. The amount of red blood cells in the blood of rabbits of experimental groups І, ІІ and ІІІ was higher respectively by 8.1%, 5.7% and 12.0% at the first stage of the study and 18.1%, 13.3%, 24.5% and 24.9% higher in animals of experimental groups І, ІІ; ІІІ and ІV at the final stage of the study compared with the control group. Hemoglobin concentration in blood of rabbits of experimental groups II and III during the study was significantly higher. Hematocrit value in blood of rabbits of experimental groups I–III was significantly higher on the 33rd day of the experiment compared with the control. Consumption of solution of microelements in the amount of 10.0 µg of I/L by the animals of experimental group III led to 15.1% higher content of total protein in the blood on the 33rd day of the study compared with the control group of animals. The level of creatinine was no higher than the physiological parameters and was 9.2%, 15.0% and 15.4% higher in the blood of rabbits of experimental groups II, III, IV on the 33rd day of the experiment compared with the control group. No significant changes in the content of triacylglycerols in the blood of rabbits of the experimental groups were seen throughout the study. However, the content of cholesterol in the blood of rabbits of experimental groups III and IV was significantly higher at the first stage compared with the control group. Further, it is practical to study physiologically substantiated amounts of solution of microelements І, Se, S in the diet of mother rabbits to determine their effect on their ability to become pregnant, reproductive function, milk production and survival of offspring.

Arthrospira platensis is a source of proteins, polysaccharides, polyunsaturated fatty acids (in tiny amounts) and phenolic components that give it different biological properties. The type of nutri...

The paper presents the results of a study of indicators that characterize the nutritional and biological value of new functional products-canned minced thorny skate with the addition of Atlantic cod – «Meatballs of stingray and cod in tomato sauce» and «Thorny skate and cod in white sauce». The content of 100 g of the product of the main food nutrients, namely, protein – from 8.4 to 10.0 g, fat – from 2.6 to 6.7 g, carbohydrates – from 10.2 to 18.1 g, depending on the recipe, was experimentally established. Studies have confirmed the high content of minor food substance – chondroitin sulfate (CS) – in canned thorny skate from 250 to 270 mg per 100 g of the product, which provides the recommended level of adequate consumption from 35.7 to 38.6%. On the basis of experimental data on the amino acid composition of the protein of new products, the indicators characterizing the biological value are calculated. Based on the results of the study, a reasonable conclusion was made about the high nutritional value and the presence of functional properties in canned thorny skate with the addition of Atlantic cod.

According to modern classification of trace elements, which is based on their biological significance for living organisms, selenium is classified as a group of vital or biogenic elements. As a biotic element, it has unique physico-chemical and biochemical properties and, with adequate intake into the body of farm animals and poultry has a positive effect on a number of physiological processes. The discovery of biological properties of selenium became the basis for its use first in the prevention and treatment of many diseases associated with a deficiency of this trace element, and later – as a stimulator of growth and development of young animals, as well as in order to increase egg production, poultry safety, improve the incubation characteristics of eggs and several other productive qualities. Scientists who have studied the effects of selenium on poultry have paid relatively little attention to meat quality. The effect of additives of different selenium doses in compound feed on the chemical composition, energy and biological value of Ukrainian white breed ducklings’ meat was studied in the scientific and economic experiment. Four groups of ducklings with 100 heads in each groups were formed to conduct the scientific and economic experiment. The duration of the experiment was 56 days and corresponded to the period of raising ducklings for meat. The ducklings of the first control group did not receive selenium supplementation. Selenium was additionally introduced into compound feed for poultry of the experimental groups in the following amount, mg/kg: the second group – 0.2; the third – 0.4 and the fourth – 0.6. It was established that the introduction of selenium into compound feed in the studied dose did not significantly affect to the quality of ducklings’ meat, although it had a positive effect on some indicators that characterize its chemical composition, nutritional and biological value. Among the experimental groups, ducklings of the third and fourth groups, which were injected with selenium into compound feed at the rate of 0.4 and 0.6 mg/kg, stood out in terms of meat quality.