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Your search keyword '"Bellini, Sarah"' showing total 3 results
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3 results on '"Bellini, Sarah"'

1. Spectral measurement method coupled with an optical model of microalgal cells to retrieve the cell concentration and mean diameter of culture samples in the context of culture monitoring

Author

Bellini, Sarah, Bendoula, Ryad, Le Floc'H, E., Carré, C, Roger, Jean-Michel, Information – Technologies – Analyse Environnementale – Procédés Agricoles (UMR ITAP), Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université Montpellier 2 - Sciences et Techniques (UM2), and Irstea Publications, Migration

Subjects
[SDE] Environmental Sciences, [SDE]Environmental Sciences
Abstract

International audience; This work presents a spectral measurement method coupled with an optical model of microalgal cells to retrieve the cell concentration and mean diameter of non diluted culture samples from a ballistic transmission measurement on the [400-750nm] spectral range. The AlgaSim simulation program was implemented to simulate the extinction cross sections of microalgal cells represented as spheres with concentric layers of different biological materials, and described by a set of physical and chemical parameters. The measurement system is composed of one integrating sphere specially designed to perform a sound measurement of the ballistic transmission, from which the spectral extinction coefficient of the sample algal suspension can be extracted. This study shows that from the measured extinction coefficient, by inverting AlgaSim simulation model, the concentration and mean diameter of two different monospecific microalgal cultures could be screened at different growth stages.

Published
2014

2. Spectroscopic measurement method using Adding-Doubling for advanced diagnosis in the monitoring of phytoplankton mass cultivation: interpretation with Mie theory

Author

Latrille, Eric, Bellini, Sarah, Bendoula, Ryad, Roger, Jean-Michel, Laboratoire de Biotechnologie de l'Environnement [Narbonne] (LBE), Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), Institut National de Recherche en Sciences et Technologies pour l'Environnement et l'Agriculture (IRSTEA). FRA., Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences
Published
2013

3. Potential of a spectroscopic measurement method using adding-doubling to retrieve the bulk optical properties of dense microalgal media

Author

Sarah Bellini, Ryad Bendoula, Jean-Michel Roger, Eric Latrille, Information – Technologies – Analyse Environnementale – Procédés Agricoles (UMR ITAP), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), Laboratoire de Biotechnologie de l'Environnement [Narbonne] (LBE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA), Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Bellini, Sarah, Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)

Subjects
Chlorophyll, Materials science, Light, [SDV]Life Sciences [q-bio], ALGUE, Analytical chemistry, 01 natural sciences, Quantitative Biology::Cell Behavior, 010309 optics, Tissue Culture Techniques, radiative transfer theory, DIAGNOSTIC, Critical point (thermodynamics), 0103 physical sciences, Radiative transfer, Transmittance, Microalgae, Scattering, Radiation, Sample preparation, Spectroscopy, adding-doubling, dense microalgal culture monitoring, multiple light scattering, inverse, bulk optical properties, Instrumentation, propriété optique, Cell Size, Scattering, Spectrum Analysis, 010401 analytical chemistry, SPECTROSCOPIE, 0104 chemical sciences, Computational physics, REFLECTANCE, MESURE OPTIQUE, Integrating sphere, Attenuation coefficient, [SDE]Environmental Sciences, culture d'algue, CARACTERISATION, Algorithms
Abstract

International audience; In the context of algal mass cultivation, current techniques used for the characterization of algal cells require time-consuming sample preparation and a large amount of costly, standard instrumentation. As the physical and chemical properties of the algal cells strongly affect their optical properties, the optical characterization is seen as a promising method to provide an early diagnosis in the context of mass cultivation monitoring. This article explores the potential of a spectroscopic measurement method coupled with the inversion of the radiative transfer theory for the retrieval of the bulk optical properties of dense algal samples.Total transmittance and total reflectance measurements were performed over the 380-1020nm range on dense algal samples with a double integrating sphere setup. The bulk absorption and scattering coefficients were thus extracted over the 380-1020nm range by inverting the radiative transfer theory using inverse-adding-doubling computations. The experimental results are presented and discussed: the configuration of the optical setup remains a critical point. The absorption coefficients obtained for the four samples of this study appear not to be more informative about pigment composition than would be classical methods in analytical spectroscopy, however there is a real added value in measuring the reduced scattering coefficient, as it appears to be strongly correlated to the size distribution of the algal cells.

Published
2014

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