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The present study focused on the comparison of growth performance of three species of live feed (microalgae) in a fixed nutrient medium in indoor culture condition. The study was conducted at the hatchery complex of Bangladesh Fisheries Research Institute, Brackishwater Station, Paikgacha, Khulna. The order of growth pattern obtained for three microalgae were Tetraselmis sp.>Nannochlorum sp.>Nannochloropsis sp. In the three consecutive trials, highest average growth 3.47×106 ± 0.8×106, 5.08×106 ± 0.9×106 and 6.91×106 ± 1.0×106 (cells/ml) (Mean± SEM) were observed for Nannochloropsis sp., Nannochlorum sp. and Tetraselmis sp. respectively. Indoor culture condition is therefore, advantageous for Tetraselmis culture in comparison to the other studied species.