1. Methodological considerations for ghrelin isoforms assay in clinical evaluation in anorexia nervosa
- Author
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C. Tezenas du Montcel, P. Duriez, N. Lebrun, D. Grouselle, B. de Grimaudet, R. Dardennes, J. Epelbaum, M. Cuenca, O. Viltart, P. Gorwood, and V. Tolle
- Subjects
Embryology ,Cell Biology ,Anatomy ,Developmental Biology - Abstract
The growing interest concerning the role of metabolic sensors in various eating disorders requires the implementation of a strict methodology to collect, store and process blood samples in clinical studies. In particular, measurement of isoforms of the appetite-stimulating hormone, ghrelin, has been challenging in clinical settings. Indeed the acyl ghrelin (AG) isoform is rapidly degraded into desacyl ghrelin (DAG) by blood esterases, thus optimal conditions for the conservation of AG and accurate determination of AG/DAG ratio should be used. Here, we compared different protease inhibitors (Aprotinin, PHMB, AEBSF) during blood collection, increasing delays (0-180 min) before centrifugation, plasma supplementation with various HCl concentrations, storage durations of frozen plasma (8 and 447 days) and immunoenzyme-assay procedures (one-step
- Published
- 2022