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Analysis of the link between the Soviet occupation of East Germany and internal resistance within the German Democratic Republic reveals that ongoing payment of reparations by East Germans out of local production—via the Soviet’s ownership of prominent local companies—affected both the incidence and the intensity of unrest at the precinct level during the uprising of June 17, 1953. This result is robust when controlling for variation in the presence of Soviet military bases and deaths in Soviet nkvd Special Camps, as well as a host of regional factors potentially correlated with differences in unrest.

We examine how the decision‐making of political elites respond to an imminent external threat to the existence of the state in times of war. To do so, we exploit exogeneous variation in exposure to battle deaths and bombing raids to estimate the effect of variation in the intensity of war on the probability that individuals charged with treason and/or high treason in Nazi Germany received the death sentence. A doubling of the number of military fatalities as well as bombing raids in the same week in which a defendant was sentenced increased the likelihood of receiving the death penalty by about 10 percentage points. (JEL K14, N44)

Objective This study examined how well healthcare providers perceive and understand their patients' health beliefs and values compared to patients' actual beliefs, and to determine if communication relationships maybe improved as a result of healthcare providers' understanding of their patients' illness from their perspective. Methods A total of 61 participants (7 healthcare providers and 54 patients) were enrolled in the study. Healthcare providers and patients individually completed survey instruments and each participated in a structured focus group. Results Healthcare provider and patient differences revealed that patients perceived greater meaning of their illness (p = 0.038), and a greater preference for partnership (p = 0.026) compared to providers. The three qualitative themes most important for understanding patients' health beliefs and values as perceived by healthcare providers were education, trust, and culture. Educating patients was perceived as having the greatest impact and also the easiest method to implement to foster providers' understanding, with at least one patient focus group in agreement of same. Likewise, three themes were derived from patients' perspectives as relatively more important in understanding providers' beliefs and values; bidirectional communication, comprehensive treatment, and discipline. Overwhelmingly, bidirectional communication was perceived as a critical factor as having the greatest impact and may also be easiest to implement according to these patients. Conclusion When patients and healthcare providers listen and communicate with each other, they are likely to develop a shared understanding that may improve future decision making and quality of care patients receive.

We examine the role of judicial policy preferences in influencing whether judges in Nazi Germany sentenced defendants charged with serious political offences to death. We find that judicial policy preferences, measured by the depth of the ideological commitment of the judge to the Nazi Party worldview, were an important determinant of whether judges imposed the death sentence. Judges more committed to the Nazi Party were more likely to impose the death sentence on defendants belonging to organised political opposition groups, those accused of violent resistance and those with characteristics to which Nazism was intolerant. This article is protected by copyright. All rights reserved.

The tools of econometric analysis and inferential statistics reveal that senior Nazi-era judges in pre-war Germany exhibited statistically significant levels of discretion in their sentencing of individuals convicted of high treason or treason. In fact, some of these judges, though appointed to the People’s Court to serve the Nazi state, were inclined to show relative leniency, within certain political limits, when taking into account the characteristics, backgrounds, affiliations, actions, and experiences of those whom they convicted. A modicum of judicial autonomy can co-exist with dictatorship so long as it enhances the efficiency of the courts and does not impugn the regime.

This article compares the real GDP per capita of the Cape Colony and Natal between 1861 and 1909 with that of Australia's two most developed colonies, Victoria and New South Wales. Estimates of European and non-European GDP per capita for both South African colonies are also provided. Together, this information allows for the first time an evaluation of the growth performance of these important parts of the South African economy in the colonial era. The article concludes that South African performance in this period was stronger than often assumed and that by the beginning of the twentieth century European South Africans, now more fully integrated into a British World economy, operated at a level of GDP per capita that matched and in some places may have exceeded that of Australians. Non-European South Africans, however, did not share in these same advances.

Background\ud Postmastectomy radiotherapy in patients with four or more positive axillary nodes reduces breast cancer mortality, but its role in patients with one to three involved nodes is controversial. We assessed the effects of postmastectomy radiotherapy on quality of life (QOL) in women with intermediate-risk breast cancer.\ud \ud Methods\ud SUPREMO is an open-label, international, parallel-group, randomised, controlled trial. Women aged 18 years or older with intermediate-risk breast cancer (defined as pT1–2N1; pT3N0; or pT2N0 if also grade III or with lymphovascular invasion) who had undergone mastectomy and, if node positive, axillary surgery, were randomly assigned (1:1) to receive chest wall radiotherapy (50 Gy in 25 fractions or a radiobiologically equivalent dose of 45 Gy in 20 fractions or 40 Gy in 15 fractions) or no radiotherapy. Randomisation was done with permuted blocks of varying block length, and stratified by centre, without masking of patients or investigators. The primary endpoint is 10-year overall survival. Here, we present 2-year results of QOL (a prespecified secondary endpoint). The QOL substudy, open to all UK patients, consists of questionnaires (European Organisation for Research and Treatment of Cancer QLQ-C30 and QLQ-BR23, Body Image Scale, Hospital Anxiety and Depression Scale [HADS], and EQ-5D-3L) completed before randomisation, and at 1, 2, 5, and 10 years. The prespecified primary outcomes within this QOL substudy were global QOL, fatigue, physical function, chest wall symptoms, shoulder and arm symptoms, body image, and anxiety and depression. Data were analysed by intention to treat, using repeated mixed-effects methods. This trial is registered with the ISRCTN registry, number ISRCTN61145589.\ud \ud Findings\ud Between Aug 4, 2006, and April 29, 2013, 1688 patients were enrolled internationally and randomly assigned to receive chest wall radiotherapy (n=853) or not (n=835). 989 (79%) of 1258 patients from 111 UK centres consented to participate in the QOL substudy (487 in the radiotherapy group and 502 in the no radiotherapy group), of whom 947 (96%) returned the baseline questionnaires and were included in the analysis (radiotherapy, n=471; no radiotherapy, n=476). At up to 2 years, chest wall symptoms were worse in the radiotherapy group than in the no radiotherapy group (mean score 14·1 [SD 15·8] in the radiotherapy group vs 11·6 [14·6] in the no radiotherapy group; effect estimate 2·17, 95% CI 0·40–3·94; p=0·016); however, there was an improvement in both groups between years 1 and 2 (visit effect −1·34, 95% CI −2·36 to −0·31; p=0·010). No differences were seen between treatment groups in arm and shoulder symptoms, body image, fatigue, overall QOL, physical function, or anxiety or depression scores.\ud \ud Interpretation\ud Postmastectomy radiotherapy led to more local (chest wall) symptoms up to 2 years postrandomisation compared with no radiotherapy, but the difference between groups was small. These data will inform shared decision making while we await survival (trial primary endpoint) results.\ud \ud Funding\ud Medical Research Council, European Organisation for Research and Treatment of Cancer, Cancer Australia, Dutch Cancer Society, Trustees of Hong Kong and Shanghai Banking Corporation.

This study examined how well healthcare providers perceive and understand their patients' health beliefs and values compared to patients' actual beliefs, and to determine if communication relationships maybe improved as a result of healthcare providers' understanding of their patients' illness from their perspective.A total of 61 participants (7 healthcare providers and 54 patients) were enrolled in the study. Healthcare providers and patients individually completed survey instruments and each participated in a structured focus group.Healthcare provider and patient differences revealed that patients perceived greater meaning of their illness (p = 0.038), and a greater preference for partnership (p = 0.026) compared to providers. The three qualitative themes most important for understanding patients' health beliefs and values as perceived by healthcare providers were education, trust, and culture. Educating patients was perceived as having the greatest impact and also the easiest method to implement to foster providers' understanding, with at least one patient focus group in agreement of same. Likewise, three themes were derived from patients' perspectives as relatively more important in understanding providers' beliefs and values; bidirectional communication, comprehensive treatment, and discipline. Overwhelmingly, bidirectional communication was perceived as a critical factor as having the greatest impact and may also be easiest to implement according to these patients.When patients and healthcare providers listen and communicate with each other, they are likely to develop a shared understanding that may improve future decision making and quality of care patients receive.

Recent literature suggests that there was a marked intensification of consumerism in the Anglophone world in the latter half of the nineteenth century, though little systematic empirical evidence of the phenomenon or its origins has to date been provided. This paper develops an economic model to redress this situation. Using a fixed-effects panel data model, it shows that the enduring racial ties, cultural affinity and sense of connectedness of British emigrants in Australasia, Canada and the US between 1879 and 1913, as evidenced by their remittance flows, were reflected in a strong preference for consuming British products.

This article uses the court records of a sample of individuals, aged between 15 and 62, tried for high treason in Nazi Germany to analyze a rare, real-world prisoner's-dilemma-like scenario that resisters faced once taken into custody: keep quiet and protect their collaborators or turn informant in the hope of obtaining leniency? We find that, although self-interest and defection to the authorities was the norm for most, significant rates of cooperation remained. We also find evidence that the size of the stake, age, education, beliefs, affiliations, and sense of community could play roles in facilitating cooperative behavior.

This article explores whether deregulation of the Australian retail banking sector in the 1980s delivered the enhanced consumer choice that had been promised. Using new data on banking products and their usage, it analyses consumers' ability to select optimal ‘frontier’ products. It concludes that following deregulation of retail banking, product offerings underwent such tumultuous change that the scope for effective consumer choice was severely constrained. While there were improvements towards the end of the period, progress was not assisted by the banks' strategy of proliferating and re-bundling products. Consequently, the anticipated improvements to consumer choice were slow to arrive.

Anecdotal reports suggest that application of a cool device to the back of the neck at the onset of a hot flush can afford symptomatic relief. The effects of a novel handheld mechanical cooling device in a population of perimenopausal women with moderate-to-severe vasomotor symptoms were evaluated.In this randomized, double-blind, sham-controlled pilot study, 40 perimenopausal women experiencing ≥ 7 moderate-to-severe hot flushes per day were recruited at a single university site. Women were randomized to the active (n = 20) or sham (n = 20) device, which was applied to the back of the neck with each hot flush over the 4-week treatment period. Hot flush scores were calculated based on frequency and severity of symptoms. The Carpenter Hot Flash Related Daily Interference Scale and Zung Anxiety Scale were used to evaluate impact on quality of life. At study end, participants completed an open-ended questionnaire to assess the degree of unblinding and overall subjective improvement in symptoms with use of the device.No statistically significant differences were observed between the effects of the active and sham device. However, thematic analysis of the open-ended questionnaire revealed that 12/17 women (70.6%) in the active group, compared to 4/18 (22.2%) women in the sham group felt the device provided some symptomatic relief.Although the majority of women using the active device acknowledged that its cooling effect afforded a degree of symptomatic relief, the symptom scores chosen for this pilot study did not reflect a beneficial effect.

Since the end of the Second World War, historical research on German and Austrian resistance has steadily expanded, deepened and enrichened our understanding of the phenomenon. Like all healthy fields of research, though, mysteries and gaps not only persist but are constantly being opened up. This book has sought to add to that accumulated knowledge by attempting to tease further insights from the archives with quantitative analysis. Its results have both cast light on a number of important existing debates and identified new areas of study that call for greater research. The various threads discovered in earlier chapters are brought together in this concluding chapter, and some of the key implications of our findings for the understanding of serious resistance activities in Nazi Germany are discussed. One new thing that emerges from this analysis is a framework that for the first time allows the objective evaluation of the impact of serious resistance to be made, an approach which with simple modifications could be easily extended to the study of dissent, opposition and nonconformity.

We study the effect of legally irrelevant events on the sentencing outcomes of around 2,500 individual defendants, heard before the People's Court in Nazi Germany. Our analysis exploits exogeneous variation in battle deaths and estimates their effect on the likelihood of receiving the death penalty. According to our results, higher German fatalities on the battlefield systematically increased the chances of receiving the death penalty. We show that decisions by experienced judges were less affected by battle deaths, while judges who were more ideologically committed to the regime were more likely to impose the death penalty in response to hearing news of higher German fatalities. Our results are not driven by particular types of offenses or defendants, time periods, or changes in arrest patterns and are robust to the use of major bombing raids of German cities instead of battle deaths. We also find some evidence that victories of the German national soccer team decreased the chances of capital punishment.

This chapter provides an overview of some of the key personal and environmental characteristics of those who participated in serious resistance to the Nazi regime. It reminds us that as a group they were more than just the politics to which they subscribed. Moreover, the chapter describes a range of interesting phenomena: inter alia, the steady aging of resisters over the course of the regime, especially during the war; the dominance of blue-collar labour in resistance movements; and the small, though disproportionate, contribution of foreigners, the stateless and people of partial Jewish ancestry in the struggle to undermine Hitler’s state from within. The chapter also demonstrates the meaningfulness of analysing resistance by stage of life and gender. In particular, its analysis highlights the distinctiveness of two often overlooked types of resistance: juvenile and female.

In this chapter, we turn our attention to another feature of the resister’s story, one that has typically been less scrutinised and certainly is not as well understood: their experience once they had been arrested and entered custody. It was there in the interrogation chambers, cells and court rooms of the Nazi regime that the personal consequences of their actions were to be first painfully brought to bear. The chapter begins with an outline of the nature and structure of the Nazi legal system and how it evolved to deal with the most serious of political crimes it confronted: treason and high treason. It then moves on to explore aspects of the reality of the resister’s actual engagement with that system. In particular, the chapter considers the length of time it took to get from arrest to sentence, the type of charges resisters were likely to face, the verdicts they could expect, the determinants of the sentence they were ultimately to receive and what role, if any, the discretion of the judge presiding over the case would play in that process. The chapter also investigates a critical choice that all resisters in custody, irrespective of their background or motivations, had to make, a choice that would directly impact their and their co-defendants’ fate: should they cooperate with the authorities or not?

Despite the personal dangers involved, a remarkable number of Germans and Austrians chose to resist the Nazi regime. The aim of this chapter is to provide a succinct, yet comprehensive, overview of the myriad groups and organisations of differing political, religious and ethnic persuasions that took the fateful decision to resist the regime. The chapter identifies precisely when, where and with what frequency each of these groups carried out their serious resistance. While the chapter captures and highlights the amazing diversity of political and spiritual beliefs which provided the organisational context for, and motivation to, those who chose to engage in serious resistance, it also identifies the enduring importance of certain parties and groups – in particular, the Communist Party of Germany (Kommunistische Partei Deutschlands or KPD) and Social Democratic Party of Germany (Sozialdemokratische Partei Deutschlands or SPD) in Germany and the Communist Party of Austria (Kommunistische Partei Osterreichs or KPO) and Catholic-Conservative-Legitimist organisations in Austria – in that struggle to bring down Hitler’s regime from within.

In this chapter, our analysis of serious internal resistance to Hitler’s regime begins with an examination of the overall structure and composition of that resistance from the inception of Nazi rule in January 1933 through to the collapse of the German legal system in early 1945. The chapter seeks to answer two fundamental questions in particular, one temporal and one geographic: namely, how did the levels of serious resistance change across time and what regions were most active in this resistance? Along the way, a number of secondary issues are also considered: inter alia, can a distinct periodisation of resistance be identified, how did the different phases of the war impact resistance activities, did the intensity of such activities in any location merely reflect its population size, and is it more appropriate to talk of the commonality or distinctiveness of regional resistance experiences? The answers to such questions gleaned from quantitative analyses of the available data on treason, and high treason shed new light on, and challenge aspects of, our understanding of the path of serious resistance within Nazi Germany. The chapter offers a new periodisation of serious resistance in Nazi Germany.

Analysis of the sixty-nine juveniles tried for high treason before the People's Court in Nazi Germany between 1933 and 1945, based on the available court records, finds that juvenile resistance in Nazi Germany possessed a distinct form and character; it was a phenomenon rather than an exceptional act. Juvenile resisters charged with high treason were typically working-class males of German ethnicity, motivated primarily by left-wing and religious beliefs, acting in small groups free of significant adult supervision and direction. Examination of the verdicts and sentencing of these juvenile resisters sheds light on how the Nazi justice system reacted to such serious internal resistance from its young.

Since the fall of the Berlin Wall, the significance of the German Democratic Republic (GDR) and all that it purported to stand for has been largely cast aside. Other than as a cautionary tale, the GDR has been widely seen as offering little to contemporary political discourse. By contrast, in recent years, its experience, especially in its early formative period, has attracted a lot of attention from historians. In part this burst of activity can be attributed to the opening of closed archives in eastern Europe, but it is also related to the desire to understand better how a flawed system could maintain such seeming stability for so long, and then, how all that could collapse so suddenly and ignobly in 1989. Was its demise inevitable, rooted, as it were, in the DNA of the system, or were there alternative paths that could have been taken? Much of the recent research is founded on the premise that insights and answers to such questions can be uncovered by going back to the origins of the system. This article is written in the same vein. Its aim is to shed light on how aspects of the East German workplace evolved in the period between the beginnings of Soviet occupation and the establishment of a Soviet-style planned economy by 1949–50.

Candida albicans is a diploid organism that exhibits high levels of heterozygosity. Although the precise manner by which this heterozygosity provides advantage for the commensal/pathogenic life styles of C. albicans is not known, heterozygous markers are themselves useful for studying genomic rearrangements, which occur frequently in C. albicans. Treatment of CAI-4 with UV light yielded histidine auxotrophs which could be complemented by HIS4, suggesting that strain CAI-4 is heterozygous for HIS4. These auxotrophs appeared to have undergone mitotic recombination and/or chromosome loss. As expected from a heterozygote, disruption of the functional allele of HIS4 resulted in a his4::hisG-URA3-hisG strain that is auxotrophic for histidine. Sequencing of random clones of the HIS4 ORF from CAI-4 and its precursor SC5314 revealed the presence of 11 SNPs, seven synonymous and four non-synonymous. Site-directed mutagenesis indicates that only one of those SNPs, T929G (Gly310Val), is responsible for the non-functionality of the encoded enzyme. HIS4 analysis of five commonly used laboratory strains is reported. This study provides a new, easily measured nutritional marker that can be used in future genetic studies in C. albicans.

Between 1870 and the 1950s, the volume and proportion of British exports to the Empire and Commonwealth grew steadily. Many have attributed this trend to non-market advantages allegedly rooted in imperial rule and the inherent Britishness of these markets. Quantitative methods show, however, that in most periods, other considerations, most notably the economic growth of the importing markets, were of much greater importance in explaining the pattern of British exports.

The yeast Candida albicans, a commensal colonizer and occasional pathogen of humans, has a rudimentary mating ability. However, mating is a cumbersome process that has never been observed outside the laboratory, and the population structure of the species is predominantly clonal. Here we discuss recent findings that indicate that mating ability is under selection in C. albicans, i.e. that it is a biologically relevant process. C. albicans strains can only mate after they have sustained genetic damage. We propose that the rescue of such damaged strains by mating may be the primary reason why mating ability is under selection.

The yeast Candida albicans can mate. However, in the natural environment mating may generate progeny (fusants) fitter than clonal lineages too rarely to render mating biologically significant: C. albicans has never been observed to mate in its natural environment, the human host, and the population structure of the species is largely clonal. It seems incapable of meiosis, and most isolates are diploid and carry both mating-type-like (MTL) locus alleles, preventing mating. Only chromosome loss or localized loss of heterozygosity can generate mating-competent cells, and recombination of parental alleles is limited. To determine if mating is a biologically significant process, we investigated if mating is under selection. The ratio of nonsynonymous to synonymous mutations in mating genes and the frequency of mutations abolishing mating indicated that mating is under selection. The MTL locus is located on chromosome 5, and when we induced chromosome 5 loss in 10 clinical isolates, most of the resulting MTL-homozygotes could mate with each other, producing fusants. In laboratory culture, a novel environment favoring novel genotypes, some fusants grew faster than their parents, in which loss of heterozygosity had reduced growth rates, and also faster than their MTL-heterozygous ancestors—albeit often only after serial propagation. In a small number of experiments in which co-inoculation of an oral colonization model with MTL-homozygotes yielded small numbers of fusants, their numbers declined over time relative to those of the parents. Overall, our results indicate that mating generates genotypes superior to existing MTL-heterozygotes often enough to be under selection.

Britain of the nineteenth century was a net recipient of migrant remittances. Surprisingly little, however, is known about the flow of such funds to the UK. This article addresses this hiatus in several ways. First, it provides an account of the main mechanisms by which remittances were transferred in this period. Second, it presents new estimates of the volume of remittances flowing to Britain between 1875 and 1913, and, in doing so, offers a comparison of remittance patterns between different Anglophone societies. Third, it assesses the significance of remittances for their recipients in the UK. The article ends by considering the implications of all of the above for the way in which historians are currently trying to formulate the concept of a ‘British world’.

Candida albicans is the most common fungal pathogen of humans. The recent discovery of sexuality in this organism has led to the demonstration of a mating type locus which is usually heterozygous, although some isolates are homozygous. Tetraploids can be formed between homozygotes of the opposite mating type. However, the role of the mating process and tetraploid formation in virulence has not been investigated. We describe here experiments using a murine model of disseminated candidiasis which demonstrate that in three strains, including CAI-4, the most commonly used strain background, tetraploids are less virulent than diploids and can undergo changes in ploidy during infection. In contrast to reports with other strains, we find that MTL homozygotes are almost as virulent as the heterozygotes. These results show that the level of ploidy in Candida albicans can affect virulence, but the mating type configuration does not necessarily do so.

The size of the genome in the opportunistic fungus Candida albicans is 15.6 Mb. Whole-genome shotgun sequencing was carried out at Stanford University where the sequences were assembled into 412 contigs. C. albicans is a diploid basically, and analysis of the sequence is complicated due to repeated sequences and to sequence polymorphism between homologous chromosomes. Chromosome 7 is 1 Mb in size and the best characterized of the 8 chromosomes in C. albicans. We assigned 16 of the contigs, ranging in length from 7309 to 267,590 bp, to chromosome 7 and determined sequences of 16 regions. These regions included four gaps, a misassembled sequence, and two major repeat sequences (MRS) of >16 kb. The length of the continuous sequence attained was 949,626 bp and provided complete coverage of chromosome 7 except for telomeric regions. Sequence analysis was carried out and predicted 404 genes, 11 of which included at least one intron. A 7-kb indel, which might be caused by a retrotransposon, was identified as the largest difference between the homologous chromosomes. Synteny analysis revealed that the degree of synteny between C. albicans and Saccharomyces cerevisiae is too weak to use for completion of the genomic sequence in C. albicans.

Economists have typically not devoted much attention to the act of invention. This paper attempts to redress this situation by exploring a form of cognition, analogical transfer, which is thought by some researchers to lie at the heart of successful creativity. An analogical transfer is said to have occurred when information and experiences from one known situation is retrieved and utilized in the search for the solution to an entirely different situation. This paper shows how such analogical thought can give rise to a theoretical framework, in which disparate factors pertaining to technological creativity can be pieced together to yield an explanation of the level of inventive output experienced.

Transnational expansion and the establishment of settlements in other parts of the world, invariably at the expense of the Indigenous Peoples, have been both regular and defining features of global...

Most Candida albicans strains are heterozygous at the MTL (mating-type-like) locus, but mating occurs in hemi- or homozygous strains. The white-opaque switch process is repressed by the heterodimer of the MTLa1 and MTLalpha2 gene products, while mating genes are induced by a2 and alpha1. Mating occurs in opaque cells and produces tetraploid progeny. A small percentage (3-7%) of clinical isolates are homozygous at the MTL locus and most are mating-competent. MTL gene expression is controlled in part by a gene which activates MTLalpha genes and represses MTLa genes in response to hemoglobin. A failure to find meiosis and the lack of evidence of mating in vivo, together with some of the properties of opaque cells, leads to the suggestion that mating may have persisted because the tightly associated switch facilitates the commensal lifestyle of this fungus.

Summary One hundred and twenty Candida albicans clinical isolates from the late 1980s and early 1990s were examined for homozygosity at the MTL locus. Of these, 108 were heterozygous (MTLa/MTLα), whereas seven were MTLa and five were MTLα. Five of the homozygous isolates were able to switch to the opaque cell morphology, while opaque cells were not detectable among the remaining seven. Nevertheless, all but one of the isolates homozygous at the MTL locus were shown to mate and to yield cells containing markers from both parents; the non-mater was found to have a frameshift in the MTLα1 gene. In contrast to Saccharomyces cerevisiae, C. albicans homozygotes with no active MTL allele failed to mate rather than mating as a cells. There was no correlation between homozygosity and fluconazole resistance, mating and fluconazole resistance or switching and fluconazole resistance, in part because most of the strains were isolated before the widespread use of this antifungal agent, and only three were in fact drug resistant. Ten of the 12 homozygotes had rearranged karyotypes involving one or more homologue of chromosomes 4, 5, 6 and 7. We suggest that karyotypic rearrangement, drug resistance and homozygosity come about as the result of induction of hyper-recombination during the infection process; hence, they tend to occur together, but each is the independent result of the same event. Furthermore, as clinical strains can mate and form tetraploids, mating and marker exchange are likely to be a significant part of the life cycle of C. albicans in vivo.

Although increases in chromosome copy number typically have devastating developmental consequences in mammals, fungal cells such as Saccharomyces cerevisiae seem to tolerate trisomies without obvious impairment of growth. Here, we demonstrate that two commonly used laboratory strains of the yeast Candida albicans, CAI-4 and SGY-243, can carry three copies of chromosome 1. Although the trisomic strains grow well in the laboratory, Ura + derivatives of CAI-4, carrying three copies of chromosome 1, are avirulent in the intravenously inoculated mouse model, unlike closely related strains carrying two copies of chromosome 1. Furthermore, changes in chromosome copy number occur during growth in an animal host and during growth in the presence of growth-inhibiting drugs. These results suggest that chromosome copy number variation provides a mechanism for genetic variation in this asexual organism.

The belief that Britain's empire markets were soft is well entrenched in the literature. It is, however, a belief that has been largely untested. Indeed, the literature does not even offer an explicit definition of softness. This article attempts to fill this gap by discussing the meaning of the term and then posing the question whether between 1870 and 1914 Britain's fastest growing markets—Australasia and Canada—can in fact reasonably be labelled soft, as has often been assumed. The article concludes that the demand for British imports in these markets was driven more by considerations of income and price than by colonial sentiment or preference.

Candida albicans is the single, most frequently isolated human fungal pathogen. As with most fungal pathogens, the factors which contribute to pathogenesis in C. albicans are not known, despite more than a decade of molecular genetic analysis. Candida albicans was thought to be asexual until the discovery of the MTL loci homologous to the mating type (MAT) loci in Saccharomyces cerevisiae led to the demonstration that mating is possible. Using Candida albicans mutants in genes likely to be involved in mating, we analysed the process to determine its similarity to mating in Saccharomyces cerevisiae. We examined disruptions of three of the genes in the MAPK pathway which is involved in filamentous growth in both S. cerevisiae and C. albicans and is known to control pheromone response in the former fungus. Disruptions in HST7 and CPH1 blocked mating in both MTLa and MTL(alpha) strains, whereas disruptions in STE20 had no effect. A disruption in KEX2, a gene involved in processing the S. cerevisiae pheromone Mf(alpha), prevented mating in MTL(alpha) but not MTLa cells, whereas a disruption in HST6, the orthologue of the STE6 gene which encodes an ABC transporter responsible for secretion of the Mfa pheromone, prevented mating in MTLa but not in MTL(alpha) cells. Disruption of two cell wall genes, ALS1 and INT1, had no effect on mating, even though ALS1 was identified by similarity to the S. cerevisiae sexual agglutinin, SAG1. The results reveal that these two diverged yeasts show a surprising similarity in their mating processes.