Search

Supplemental material, sj-pdf-1-vdi-10.1177_10406387231164368 for T-cell–rich, large B-cell lymphoma in the brain of a horse by Daniel R. Rissi, Anne C. Avery and Robert C. Burnett in Journal of Veterinary Diagnostic Investigation

Supplemental material, sj-pdf-1-vdi-10.1177_10406387221139799 for A series of heterogeneous lymphoproliferative diseases with CD3 and MUM1 co-expressed in cats and dogs by Kelly L. Hughes, Emily D. Rout, Paul R. Avery, Alana A. Pavuk, Anne C. Avery and A Russell Moore in Journal of Veterinary Diagnostic Investigation

Supplemental material, sj-pdf-1-vdi-10.1177_10406387221139799 for A series of heterogeneous lymphoproliferative diseases with CD3 and MUM1 co-expressed in cats and dogs by Kelly L. Hughes, Emily D. Rout, Paul R. Avery, Alana A. Pavuk, Anne C. Avery and A Russell Moore in Journal of Veterinary Diagnostic Investigation

Additional file 10. Multidimensional scaling plot showing clustering of European dogs. Dogs marked in red met our threshold for potential European origin based on clustering and were subsequently removed from the analysis.

Additional file 9. Flow cytometric analysis of peripheral blood samples. (A) Sample considered diagnostic for TZL due to homogeneous expansion of CD5+CD45− T cells (red cells). (B) Sample diagnosed as TZUS due to smaller population of CD5+CD45− T cells and absence of lymphocytosis or lymphadenopathy. (C) Sample considered a control; all T cells are CD5 + CD45+ (green cells; normal).

Additional file 6. GWA for combined TZL and MCT datasets. QQ-plot (left) and Manhattan plot (right). A) GWAS of combined TZL and MCT cases versus TZUS and TZL controls; B) GWAS of TZL cases versus combined TZUS, TZL controls, and MCT controls.

Additional file 5. Multidimensional scaling plot for combined TZL and MCT datasets. Plot is colored by phenotype and dataset.

Additional file 1. GWA for all TZL combinations. QQ-plot (left) and Manhattan plot (right). A) TZL case + TZUS vs. control; B) TZL case vs. control; C) TZL case vs. TZUS.

The goal of environmental enrichment for laboratory animals is to improve welfare, but some enrichment practices may affect research in unintended ways or even be harmful to the animals themselves. We previously found that mice raised at a commercial vendor then given multiple enrichment devices upon arrival at our facilities experienced thymic atrophy and greater variation in measured parameters than did their unenriched counterparts, suggesting that enrichment conditions affected corticosteroid expression in mice. The current study verified and expanded these results, examining 120 female BALB/c mice raised with or without nesting material at a commercial vendor (n = 60 per group) and allocated (n = 20 per group) to receive no enrichment, nesting material, or 'superenrichment' on arrival at our facilities. Nesting material provided prior to weaning was associated with higher levels of urinary corticosteroid, whereas superenrichment and nesting material during the adult period both led to increased thymic atrophy. Paradoxically, mice that never received enrichment, despite having the lowest corticosterone levels and least thymic atrophy, had increased tail wounds resulting from aggressive interactions. Therefore, enrichment devices that are as seemingly innocuous as nesting material, even if only provided in the preweaning period, may lead to significant, lasting changes in behavioral, physical, or immunologic measures with the potential to alter research outcomes.