1. Cutting Edge: Receptor-Mediated Endocytosis of Heat Shock Proteins by Professional Antigen-Presenting Cells
- Author
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Arnold-Schild, D., Hanau, D., Spehner, D., Schmid, C., Rammensee, H. G., Henri de la Salle, and Schild, H.
- Subjects
Leukemia P388 ,Immunology ,H-2 Antigens ,Receptors, Antigen, T-Cell ,Antigen-Presenting Cells ,Coated Pits, Cell-Membrane ,Endosomes ,Clathrin ,Endocytosis ,Cell Line ,Mice ,Antigens, Neoplasm ,Animals ,Immunology and Allergy ,Gold ,Heat-Shock Proteins - Abstract
Immunization with heat shock proteins (HSPs) induces Ag-specific CTL responses. The specificity of the immune response is based on peptides associated with HSPs. To investigate how exogenous HSP/peptide complexes gain access to the MHC class I-restricted Ag presentation pathway, we incubated the monocytic cell line P388D1 and the dendritic cell line D2SC/1 with gold-labeled HSPs gp96 and HSC70. We show that HSPs bind specifically to the surface of these APCs and are internalized spontaneously by receptor-mediated endocytosis, demonstrating the existence of specific receptors for HSPs on these cells. In addition, we observe colocalization of internalized HSPs and surface MHC class I molecules in early and late endosomal structures. These findings provide possible explanations for the immunogenicity of HSP/peptide complexes and for the transfer of HSP-associated peptides onto MHC class I molecules.
- Published
- 1999
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