74 results on '"Arduini F"'
Search Results
2. A paper-based electrochemical device for the detection of pesticides in aerosol phase inspired by nature: A flower-like origami biosensor for precision agriculture
- Author
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Caratelli, V, Fegatelli, G, Moscone, D, and Arduini, F
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Aerosols ,Biomedical Engineering ,Biophysics ,Environmental pollution ,Miniaturized device ,Screen-printed electrode ,Smartphone-assisted analysis ,Wax printing ,Agriculture ,General Medicine ,Biosensing Techniques ,Settore CHIM/01 ,Butyrylcholinesterase ,Electrochemistry ,Pesticides ,Biotechnology - Abstract
Pesticides are largely used at worldwide level to improve food production, fulfilling the needs of the global population which is increasing year by year. Although pesticides are beneficial for crop production, their extensive use has serious consequences for the pollution of the produced food as well as for soil and groundwaters. Indeed, it is reported that 50% of sprayed pesticides reach different destinations other than their target species, including soil, surface waters, and groundwaters. For this reason, we developed a flower-like origami paper-based device for pesticides detection in aerosol phase for precision agriculture. In detail, the paper-based electrochemical platform detects paraoxon, 2,4-dichlorophenoxyacetic acid, and glyphosate at ppb levels by measuring their inhibitory activity towards three different enzymes namely butyrylcholinesterase, alkaline phosphatase, and peroxidase enzyme, respectively. This integrated electrochemical device is composed of three office paper-based screen-printed electrodes and filter paper-based pads loaded with enzymes and enzymatic substrates. The pesticide detection is carried out by measuring through chronoamperometric technique the initial and residual enzymatic activity by using a smartphone-assisted potentiostat and evaluating the percentage of inhibition, proportional to the amount of aerosolized pesticides. This paper-based device was able to detect the three classes of pesticides in aerosol phase with limits of detection equal to 30 ppb, 10 ppb, and 2 ppb, respectively for 2,4-D, glyphosate, and paraoxon.
- Published
- 2021
3. Deep eutectic solvents with bio-based chiral cations: a new electroanalytical approach for the enantiomeric excess determination of chiral probes
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Emanuele, E, Arnaboldi, S, Grecchi, S, Guazzelli, L, Longhi, M, Arduini, F, Micheli, L, and Mussini, P
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Settore CHIM/01 ,Settore CHIM/02 - Published
- 2021
4. Nuovi elettrodi stampati paper-based per il monitoraggio dei processi degradativi del cemento armato
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Arduini, F, Colozza, N, Sassolini, A, Mazzaracchio, V, Luca, F, Kersti, H, and MOSCONE DINIA, D
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Settore CHIM/01 - Published
- 2019
5. Paper-based Electroanalytical Strip for User-Friendly Blood Glutathione Detection
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Arduini, F, Cinti, S, Tomei Maria Rita, and MOSCONE DINIA, D
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Settore CHIM/01 - Published
- 2019
6. Nuovi elettrodi per la misura del pH, integrati in dispositivi medici, utile per monitorare la presenza di infezioni
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Arduini, F, Luca, F, Mazzaracchio, V, and MOSCONE DINIA, D
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Settore CHIM/01 - Published
- 2019
7. Nuovi sensori stampati su carta nanostrutturati
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Boccella, A, Moscone, D, Arduini, F, Neagu, D, and Tomei Maria Rita
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Settore CHIM/01 - Published
- 2019
8. Monitoraggio della corrosione nelle barre di rinforzo per le strutture in cemento armato
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Arduini, F, Colozza, N, Sassolini, A, Abruzzese, D, Micheletti, A, and Tiero, A
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corrosione ,cemento armato ,barre di armatura ,sensori elettrochimici ,Settore CHIM/01 ,Settore ICAR/08 - Published
- 2019
9. Paper-Based Electrochemical Devices in Biomedical Field: Recent Advances and Perspectives
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Arduini F, Cinti S, Scognamiglio V, Moscone D, Arduini, F, Cinti, S, Scognamiglio, V, and Moscone, D
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biomedical field ,Electrochemical Inkjet Origami Paper-based device POCTs Screen printing μPAD μPED ,Settore CHIM/01 - Chimica Analitica ,paper biosensor - Abstract
In EU, electronic equipment generated 9 mill tons of waste in 2005, and it is expected to grow to more than 12 million tons by 2020. To decrease the environmental pollutions of electronics, the development of sustainable sensors, including paper-based electrochemical devices, are attracting growing interest thanks to their cost-effectiveness, suitability for in situ analysis, and environmentally friendly. The development of the paper-based devices as point of care is a paramount issue, since this type of systems meets the requirements of the ASSURED criteria coined by WHO, which describe the ideal characteristics of a diagnostic test. As a matter of fact, paper-based (bio)sensors with electrochemical detection practically match this criteria, as they are Affordable (by those at risk of infection), Sensitive (low false-negatives), Specific (few false-positives), User-friendly (simple to perform and requiring minimal training), Rapid (to give useful information at first visit) and robust (do not require refrigerated storage), Equipment-free, Delivered to those who need it. This chapter covers the recent progresses on paper-based electrochemical (bio)sensors for biomedical field, giving an overview of the different papers and techniques used for the fabrication of paper-based devices as well as the different configurations designed. The main section encompasses the description of several paper-based sensors and biosensors (i.e. enzymatic, immuno- and nucleic-acid based sensors) with biomedical applications as well. The last section describes the future perspectives for eco-designed (bio)sensors, being a hot topic in the field of analytical sensing tools.
- Published
- 2017
10. Electroanalysis moves towards paper-based printed electronics
- Author
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Cinti, S., Colozza, N., Cacciotti, I., Moscone, D., Polomoshnov, M., Sowade, E., Baumann, R.R., Arduini, F., and Publica
- Abstract
Herein we demonstrated, for the first time, the possibility to use the paper employed in printed electronics (i.e. p_e:smart) as substrate to develop a paper-based sensor. To improve the electrochemical performances of the inkjet-printed sensor, a dispersion based on carbon black nanoparticles was used to modify the working electrode, allowing for a highly performant nanomodified electrochemical sensor platform. This disposable sensor was characterized both electrochemically and morphologically, and it has been successively challenged towards a model analyte namely ascorbic acid. It has been evidenced that the presence of carbon black as nanomodifier decreased the overpotential for ascorbic acid oxidation (from 0.47 V to 0.28 V) with respect to the unmodified sensor and boosted the sensitivity (ca. 3-times). The applicability of this printed electrochemical sensor was demonstrated for the detection of ascorbic acid in a dietary supplement, quantifying 999 ± 130 mg with respect to the 1000 mg reported on the label.
- Published
- 2018
11. Chemical vapor deposition: influence of iron catalyst in the carbon spheres synthesis for energy and electrochemical applications
- Author
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Scarselli, M, Limosani, F, Passacantando, M, D(')Orazio, F, Nardone, M, Cacciotti, I, Arduini, F, Gautron, E, and De Crescenzi, M
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Settore CHIM/03 - Published
- 2018
12. Past, Present and Future Challenges of Biosensors and Bioanalytical Tools in Analytical Chemistry: A Tribute to Professor Marco Mascini
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Arduini, F, Cinti, S, Viviana, S, and MOSCONE DINIA, D
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Settore CHIM/01 - Chimica Analitica - Published
- 2017
13. Commercially Available (Bio)sensors in the Agrifood Sector
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Antonacci A., Arduini F., Moscone D., Palleschi G., and Scognamiglio V.
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agrifood sector ,commercial biosensors - Abstract
A paramount research is focused nowadays on agrifood analysis, to ensure the commercialisation of safe products that meet the requirements of consumer perception. Food manufacturing processes might be threaten by contamination or lack of quality at each stage of the food supply chain while growing, processing, packaging, or distributing. Therefore, there is an increasing demand for fast and automatic analytical tools suitable for real-time control of food quality and safety through the whole chain. (Bio)sensors have high potential to this concern, being fast and sensitive analytical methods for the analysis of food composition and contamination, as well as for online process control. In this review, commercial (bio)sensors available for the agrifood sector are reported and compared in terms of their crucial features tailored for the different agrifood sector segments, including food analysis, process control, intelligent packaging, and smart agriculture. Moreover, an overview on the last trends in biosensor technologies is reported with the aim to enhance the biosensor performance and to develop newer sensor design and construction, fostering food production processes at high-quality standards.
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- 2016
14. SCREEN‐PRINTED ELECTRODES MODIFIED WITH CARBON NANOMATERIALS: A CHALLENGE AMONG CARBON BLACK, CARBON NANOTUBES AND GRAPHENE
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Arduini, F, Cinti, S, Carbone, M, Sansone, L, Cacciotti, I, MOSCONE DINIA, D, and Palleschi, G
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Settore CHIM/03 - Chimica Generale e Inorganica ,Settore CHIM/01 - Chimica Analitica - Published
- 2015
15. An acetylcholinesterase biosensor for determination of low concentrations of Paraoxon and Dichlorvos
- Author
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Di Tuoro, D, Portaccio, M, Lepore, M, Vitiello, A, Arduini, F, MOSCONE DINIA, D, Bencivenga, U, Mita, Dg, DI TUORO, D, Portaccio, Marianna Bianca Emanuela, Lepore, Maria, Arduini, F, Moscone, D, Bencivenga, U, and Mita, D. G.
- Subjects
Insecticides ,concentration ,dichlorvo ,tmb ,Bioengineering ,Biosensing Techniques ,base biosensor ,biosensor ,chemistry.chemical_compound ,Limit of Detection ,Bromide ,Dichlorvos ,pam ,medicine ,ache ,acetylthiocholine ,Animals ,Humans ,Organic chemistry ,Settore CHIM/01 - Chimica Analitica ,ppb ,Molecular Biology ,pesticide ,Chromatography ,Paraoxon ,paraoxon ,acetylcholinesterase ,paste ,current intensity ,electrode ,Substrate (chemistry) ,Electrochemical Techniques ,General Medicine ,Enzymes, Immobilized ,Oxime ,Acetylcholinesterase ,chemistry ,Acetylthiocholine ,Cholinesterase Inhibitors ,Oxidation-Reduction ,Biosensor ,Biotechnology ,medicine.drug - Abstract
The characterization of an economic and ease-to-use carbon paste acetylcholinesterase (AChE) based biosensor to determine the concentration of pesticides Paraoxon and Dichlorvos is discussed. AChE hydrolyses acetylthiocholine (ATCh) in thiocoline (TC) and acetic acid (AA). When AChE is immobilized into a paste carbon working electrode kept at +410 mV vs. Ag/AgCl electrode, the enzyme reaction rate using acetylthiocholine chloride (ATCl) as substrate is monitored as a current intensity. Because Paraoxon and Dichlorvos inhibit the AChE reaction, the decrease of the current intensity, at fixed ATCl concentration, is a measure of their concentration. Linear calibration curves for Paraoxon and Dichlorvos determination have been obtained. The detection limits resulted to be 0.86 ppb and 4.2 ppb for Paraoxon and Dichlorvos, respectively, while the extension of the linear range was up 23 ppb for the former pesticide and up to 33 ppb for the latter. Because the inhibited enzyme can be reactivated when immediately treated with an oxime, the biosensor reactivation has been studied when 1,1'-trimethylene bis 4-formylpyridinium bromide dioxime (TMB-4) and pyridine 2-aldoxime methiodide (2-PAM) were used. TMB-4 resulted more effective. The comparison with the behavior of similar AChE based biosensors is also presented.
- Published
- 2011
16. High performance electrochemical sensor based on modified screen-printed electrodes with cost-effective dispersion of nanostructured carbon black
- Author
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ARDUINI, F, AMINE, A, MAJORANI, C, DI GIORGIO, F, D, CATALDO, F, MOSCONE, D, PALLESCHI, DE FELICIS, DANIELE, Arduini, F, Amine, A, Majorani, C, Di, Giorgio, F, DE FELICIS, Daniele, D, Cataldo, F, Moscone, and D, Palleschi
- Published
- 2010
17. A thionine-modified carbon paste amperometric biosensor for cathecol and Bisphenol A determination
- Author
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PORTACCIO, Marianna Bianca Emanuela, LEPORE, Maria, DIANO, Nadia, DI TUORO D., ARDUINI F., MITA D. G., MITA L., MOSCONE D., Portaccio, Marianna Bianca Emanuela, DI TUORO, D., Arduini, F., Lepore, Maria, Mita, D. G., Diano, Nadia, Mita, L., and Moscone, D.
- Subjects
Catechol ,Bisphenol A Tyrosinase Endocrine disruptor Thionine - Abstract
A thionine-modified carbon paste electrode for catechol and Bisphenol A (BPA) detection is presented. Graphite powder was modified by adsorbing thionine as electrochemical mediator. The electrochemical response of the modified carbon paste electrode (CPE) was determined before electrode modification with tyrosinase. Then, tyrosinase was added in order to assemble a biosensor. Once established the best operative conditions, an interelectrode reproducibility around 7% was obtained and the resulting biosensor showed improved sensitivities and (S = 139.6±1.1 nA/*M for catechol and S = 85.4±1.5 nA/*M for BPA) in comparison with the biosensor constructed without thionine (S = 104.4±0.5 nA/*M for catechol and S = 51.1±0.6 nA/*Mfor BPA) and low detection limits (0.15*Mfor both the electrodes and analytes). Also the comparison with the results reported in the literature showed higher sensitivity and lower detection limit for our biosensor. Moreover the functioning of the thionine-tyrosinase CPE was validated following a biodegradation process of water polluted by BPA and comparing the time changes of BPA concentration inferred by the biosensor calibration curve and those determined by means of HPLC measurements
- Published
- 2010
18. Biosensing technology for a sustainable food safety (review)
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Scognamiglio, V, Arduini, F, Palleschi, G, and Rea, G
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Settore CHIM/01 - Chimica Analitica - Published
- 2014
19. Analytical optimizations of a procedure for lead detection in milk by means of bismuth-modified screen-printed electrodes (part two)
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Calvo Quintana, J, Arduini, F, Amine, A, van Velzen, K, Palleschi, G, and MOSCONE DINIA, D
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Settore CHIM/01 - Chimica Analitica - Published
- 2012
20. A rapid enzymatic method for aflatoxin B detection
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MOSCONE DINIA, D, Arduini, F, and Amine, A
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Aflatoxins ,Acetylcholinesterase ,Food Contamination ,Settore CHIM/01 - Chimica Analitica - Published
- 2011
21. Development of a biosensor for AFB1 detection in olive oil samples
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Ben Rejeb, I, Arduini, F, Arvinte, A, Amine, A, MOSCONE DINIA, D, Gargouri, M, and Palleschi, G
- Subjects
Acetylcholinesterase Aflatoxin B1 Inhibition Prussian Blue Screen-printed electrode Olive oil ,Settore CHIM/01 - Chimica Analitica - Published
- 2009
22. Metodi di analisi basati sull’inibizione delle colinestrerasi per applicazioni nel campo ambientale e alimentare
- Author
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Arduini, F
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aflatoxins ,cholinesterase inhibition ,pesticides ,nerve agents ,Settore CHIM/01 - Chimica Analitica - Published
- 2008
23. Biosensori per la determinazione enzimatica del Trealosio
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Antonelli, Marta Letizia, Bruno, C., and Arduini, F.
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- 2008
24. Bioremediation and biodetermination of bisphenol A (BPA) in aqueous solutions
- Author
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Mita, Dg, Attanasio, A, Diano, N, Grano, V, Bencivenga, U, Rossi, S, Canciglia, P, Mita, L, Portaccio, M, Arduini, F, Amine, A, and MOSCONE DINIA, D
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Settore CHIM/01 - Chimica Analitica - Published
- 2007
25. BIOREMEDIATION AND BIODETERMINATION OF BISPHENOL A (BPA) IN AQUEOUS SOLUTION
- Author
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Mita, Dg, Attanasio, A, Diano, N, Grano, V, Bencivenga, U, Rossi, S, Canciglia, Paolo, Mita, L, Portaccio, M, Arduini, F, Amine, A, and Moscone, D.
- Published
- 2007
26. Utilità dell’anestesia pericapsulare in corso di biopsia prostatica transperineale sotto guida ecografica
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Drudi, Francesco Maria, Marchetti, F, Mendicino, Palma, Pasqualini, V, Filpo, Marzia, Profiti, R, Arduini, F, and Cantisani, Vito
- Published
- 2004
27. The G/A nucleotide change at cDNA position 2494 in the E-cadherin gene (CDH1): analysis in Italian patients
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Ruzzo, Annamaria, Graziano, F, Costagliola, A, Arduini, F, Testa, E, Bearzi, I, Cortese, G, DE GAETANO, A, Andreoni, Francesca, Lai, V, and Magnani, Mauro
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CDH1 ,gastric cancer ,missense mutation ,E-cadherin ,germline - Abstract
Current studies are investigating new E-cadherin gene (CDH1) mutations that may be responsible for diffuse gastric cancer susceptibility. Recently, a novel CDH1 germline variant presenting a G/A nucleotide change at cDNA position 2494 has been found in Japanese patients with familial diffuse gastric cancer. The consequent amino acid variation (Val/Met) may alter the binding activity to beta-catenin and the adhesive function of the E-cadherin protein. We have investigated its frequency in Italian cases of sporadic diffuse gastric cancer a well as in healthy controls. Peripheral blood samples were collected from consecutive patients with sporadic, diffuse gastric cancer and from healthy controls in the District of Urbino, Marche Region, Central Italy. After DNA extraction, standard techniques for molecular analyses were used to investigate the 2494 G/A germline nucleotide change in CDH1 cDNA. None of the 48 patients and 48 controls showed the G/A 2494 nucleotide change. Assuming a binomial distribution of the mutation among individuals and the absence of mutations in the 48 patients, the 95% upper bound for the underlying mutation frequency was 7.4%. The novel CDH1 nucleotide change is uncommon in Italian patients with sporadic diffuse gastric cancer. Given these results, further analyses in large population-based studies are not advisable
- Published
- 2003
28. Diagnostica per immagini: evoluzione tecnologica - L'ecografia
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Ricci, Paolo, Arduini, F, and DI FILIPPO, A.
- Published
- 2002
29. [Computed tomography evaluation of metastasis of cutaneous melanoma]
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Potente G, Cantisani C, Cantisani V, Bottoni U, stefano calvieri, Gm, Andreoli, Arduini F, and Guerrisi R
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Adult ,Aged, 80 and over ,Male ,Lung Neoplasms ,Skin Neoplasms ,Adolescent ,Brain Neoplasms ,Liver Neoplasms ,Middle Aged ,Lymphatic Metastasis ,Humans ,Female ,Tomography, X-Ray Computed ,Melanoma ,Aged - Abstract
To assess the value of Computed Tomography (CT) in the diagnosis and in morphologic characterization of metastatic melanoma.The data of total body CT of 124 consecutive patients with melanoma having a Breslow index 1 mm or a positive sentinel lymph node have been retrospectively reviewed.The CT scan showed loco-regional and/or distant metastases in 36 patients (39%). Ten of these (28%) had metastases only to lymph nodes, whereas 26 patients (72%) had multiple metastases. Nodal, pulmonary, brain, subcutaneous, hepatic, adrenal, bone, gastrointestinal, breast and abdominal wall metastases were detected in 80.6%, 47.2%, 25%, 25%, 16.7%, 13.9%, 11.1%, 5.6%, 5.6% and 2.8% of the patients respectively. All the patients with metastases also had a positive sentinel lymph nodes and/or symptoms of metastatic disease.CT fails to reveal any characteristic feature of metastatic melanoma, but it is of value in the diagnosis of loco-regional and distant metastases in III stage disease.
- Published
- 2001
30. A Multilevel GMRF based approach to image segmentation and restoration
- Author
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Regazzoni, Carlo, Arduini, F., and Vernazza, Gianni
- Published
- 1993
31. MULTILEVEL MRFS FOR INTELLIGENT IMAGE SEGMENTATION
- Author
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Regazzoni, C. S., Arduini, F., and Bavazzano, M.
- Published
- 1992
32. A Numerical and Symbolic Fusion Method for Interpretation of Image Sequence
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Arduini, F, Cabri, R, Foresti, Gian Luca, Murino, V, Regazzoni, C, and Vernazza, G.
- Published
- 1991
33. Biosensors for quality and safety control of olive oil: A review
- Author
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Arduini, F., Amine, A., Moscone, D., and Giuseppe Palleschi
34. Electroanalytical Sensor Based on Gold-Nanoparticle-Decorated Paper for Sensitive Detection of Copper Ions in Sweat and Serum
- Author
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Simona Roggero, Neda Bagheri, Paolo A. Netti, Vincenzo Mazzaracchio, Stefano Cinti, Danila Moscone, Noemi Colozza, Mohammad Saraji, Fabiana Arduini, Concetta Di Natale, Bagheri, N., Mazzaracchio, V., Cinti, S., Colozza, N., Di Natale, C., Netti, P. A., Saraji, M., Roggero, S., Moscone, D., and Arduini, F.
- Subjects
Analyte ,Microfluidics ,Metal Nanoparticles ,Nanoparticle ,chemistry.chemical_element ,Nanotechnology ,Biosensing Techniques ,Standard solution ,010402 general chemistry ,01 natural sciences ,Analytical Chemistry ,law.invention ,Biosensing Technique ,law ,Ion ,Sweat ,Ions ,Filter paper ,010401 analytical chemistry ,Copper ,0104 chemical sciences ,chemistry ,Reagent ,Gold ,Atomic absorption spectroscopy - Abstract
The growth of (bio)sensors in analytical chemistry is mainly attributable to the development of affordable, effective, portable, and user-friendly analytical tools. In the field of sensors, paper-based devices are gaining a relevant position for their outstanding features including foldability, ease of use, and instrument-free microfluidics. Herein, a multifarious use of filter paper to detect copper ions in bodily fluids is reported by exploiting this eco-friendly material to (i) synthesize AuNPs without the use of reductants and/or external stimuli, (ii) print the electrodes, (iii) load the reagents for the assay, (iv) filter the gross impurities, and (v) preconcentrate the target analyte. Copper ions were detected down to 3 ppb with a linearity up to 400 ppb in standard solutions. The applicability in biological matrices, namely, sweat and serum, was demonstrated by recovery studies and by analyzing these biofluids with the paper-based platform and the reference method (atomic absorption spectroscopy), demonstrating satisfactory accuracy of the novel eco-designed analytical tool.
- Published
- 2021
35. Robot-assisted rehabilitation for children with neurological disabilities: Results of the Italian consensus conference CICERONE
- Author
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Saviola, Donatella, Castelli, Enrico, Beretta, Elena, De Tanti, Antonio, Arduini, Francesca, Biffi, Emilia, Colazza, Alessandra, Di Pede, Chiara, Guzzetta, Andrea, Lucarini, Ludovica, Maghini, Irene, Mandalà, Martina, Nespoli, Maurizio, Pavarelli, Claudia, Policastro, Francesca, Polverelli, Marco, Rossi, Andrea, Dei Bambini, Ospedale, Sgandurra, Giuseppina, Boldrini, Paolo, Bonaiuti, Donatella, Mazzoleni, Stefano, Posteraro, Federico, Benanti, Paolo, Draicchio, Francesco, Falabella, Vincenzo, Galeri, Silvia, Gimigliano, Francesca, Grigioni, Mauro, Mazzon, Stefano, Molteni, Franco, Morone, Giovanni, Petrarca, Maurizio, Picelli, Alessandro, Senatore, Michele, Turchetti, Giuseppe, Castelli, E., Beretta, E., De Tanti, A., Arduini, F., Biffi, E., Colazza, A., Di Pede, C., Guzzetta, A., Lucarini, L., Maghini, I., Mandala, M., Nespoli, M., Pavarelli, C., Policastro, F., Polverelli, M., Rossi, A., Sgandurra, G., Boldrini, P., Bonaiuti, D., Mazzoleni, S., Posteraro, F., Benanti, P., Draicchio, F., Falabella, V., Galeri, S., Gimigliano, F., Grigioni, M., Mazzon, S., Molteni, F., Morone, G., Petrarca, M., Picelli, A., Senatore, M., Turchetti, G., and Saviola, D.
- Subjects
children ,Robot ,robots ,lower limb ,Physical Therapy, Sports Therapy and Rehabilitation ,Neurology (clinical) ,upper limb ,gait ,Robots ,rehabilitation - Abstract
BACKGROUND: The use of robotic technologies in pediatric rehabilitation has seen a large increase, but with a lack of a comprehensive framework about their effectiveness. OBJECTIVE: An Italian Consensus Conference has been promoted to develop recommendations on these technologies: definitions and classification criteria of devices, indications and limits of their use in neurological diseases, theoretical models, ethical and legal implications. In this paper, we present the results for the pediatric age. METHODS: A systematic search on Cochrane Library, PEDro and PubMed was performed. Papers published up to March 1st, 2020, in English, were included and analyzed using the methodology of the Centre for Evidence-Based Medicine in Oxford, AMSTAR2 and PEDro scales for systematic reviews and RCT, respectively. RESULTS: Some positives aspects emerged in the area of gait: an increased number of children reaching the stance, an improvement in walking distance, speed and endurance. Critical aspects include the heterogeneity of the studied cases, measurements and training protocols. CONCLUSION: Many studies demonstrate the benefits of robotic training in developmental age. However, it is necessary to increase the number of trials to achieve greater homogeneity between protocols and to confirm the effectiveness of pediatric robotic rehabilitation.
- Published
- 2022
36. Paper-based electroanalytical strip for user-friendly blood glutathione detection
- Author
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Valeria Manovella, Stefano Cinti, Danila Moscone, Fabiana Arduini, Nicolò Interino, Maria Rita Tomei, Tomei, M. R., Cinti, S., Interino, N., Manovella, V., Moscone, D., and Arduini, F.
- Subjects
Blood, Reagent-free, Screen-printed electrodes, Self-care, Wax printing ,Materials science ,Screen-printed electrodes ,02 engineering and technology ,Overpotential ,010402 general chemistry ,01 natural sciences ,chemistry.chemical_compound ,Conductive ink ,Materials Chemistry ,Settore CHIM/01 - Chimica Analitica ,Electrical and Electronic Engineering ,Process engineering ,Instrumentation ,Detection limit ,Reagent-free ,Prussian blue ,Nanocomposite ,Filter paper ,business.industry ,Metals and Alloys ,Repeatability ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Blood ,chemistry ,Screen-printed electrode ,Self-care ,0210 nano-technology ,business ,Sensitivity (electronics) ,Wax printing - Abstract
Paper-based devices are always more gaining a relevant position in the field of sensors. The continuous demand for affordable, simple, sustainable, and portable devices, is making paper as the ideal basis towards the realization of analytical tools for the easy self-testing. In this work, we demonstrate, for the first time, the development of a disposable paper-based printed electroanalytical strip for reliable, rapid, and high-throughput detection of glutathione in blood. The detection is based on the thiol-disulfide exchange reaction, which produces a detectable compound easily oxidizable at a Prussian Blue/carbon black nanocomposite involving a favorable low-interference overpotential. This nanocomposite is mixed within a carbon-based conductive ink and successively screen-printed onto a wax-patterned filter paper. The employment of paper provides a reagent-free device, as a consequence of the reagents pre-loading within the testing area. After the experimental conditions have been optimized, glutathione has been detected up to 10 mM, with a detection limit of 60 μM, and a sensitivity of (0.102 ± 0.005) μA/mM. This sensor showed satisfactory repeatability (relative standard deviation equal to 10%, for detection of glutathione 1 mM), especially by considering the hand-made manufacturing process. The “real-world” applicability of this strip has been evaluated by quantifying blood glutathione at physiological levels and by recovery studies achieving satisfactory values.
- Published
- 2019
37. Preparation of paper-based devices for reagentless electrochemical (bio)sensor strips
- Author
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Fabiana Arduini, Stefano Cinti, Danila Moscone, Cinti, S., Moscone, D., and Arduini, F.
- Subjects
Paper ,Computer science ,Nanotechnology ,Biosensing Techniques ,STRIPS ,Reference electrode ,General Biochemistry, Genetics and Molecular Biology ,Phosphates ,law.invention ,03 medical and health sciences ,Software portability ,Silver chloride ,chemistry.chemical_compound ,Settore CHIM/01 ,0302 clinical medicine ,law ,Electrodes ,030304 developmental biology ,0303 health sciences ,Electrochemical Techniques ,Equipment Design ,chemistry ,Filter (video) ,Electrode ,Screen printing ,Biosensor ,030217 neurology & neurosurgery - Abstract
Despite substantial advances in sensing technologies, the development, preparation, and use of self-testing devices is still confined to specialist laboratories and users. Decentralized analytical devices will enormously impact daily lives, enabling people to analyze diverse clinical, environmental, and food samples, evaluate them and make predictions to improve quality of life, particularly in remote, resource-scarce areas. In recent years, paper-based analytical tools have attracted a great deal of attention; the well-known properties of paper, such as abundance, affordability, lightness, and biodegradability, combined with features of printed electrochemical sensors, have enabled the development of sustainable devices that drive (bio)sensors beyond the state of the art. Their blindness toward colored/turbid matrices (i.e., blood, soil), their portability, and the capacity of paper to autonomously filter/purge/react with target species make such devices powerful in establishing point-of-need tools for use by non-specialists. This protocol describes the preparation of a voltammetric phosphate sensor and an amperometric nerve agent biosensor; both platforms produce quantitative measurements with currents in the range of microamperes. These printed strips comprise three electrodes (graphite for working and counter electrodes and silver/silver chloride (Ag/AgCl) for the reference electrode) and nanomodifiers (carbon black and Prussian blue) to improve their performance and specificity. Depending on analytical need, different types of paper (filter, office) and configurations (1D, 2D, 3D) can be adopted. The protocol, based on the use of cost-effective manufacturing techniques such as drop casting (to chemically modify the substrate surface) and wax/screen printing (for creating the channels and electrodes), can be completed in
- Published
- 2019
38. Multi-array wax paper-based platform for the pre-concentration and determination of silver ions in drinking water
- Author
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Eleonora Nobile, Neda Bagheri, Danila Moscone, Stefano Cinti, Fabiana Arduini, Bagheri, N., Cinti, S., Nobile, E., Moscone, D., and Arduini, F.
- Subjects
Paper-based ,Inorganic chemistry ,Nanoparticle ,02 engineering and technology ,01 natural sciences ,Analytical Chemistry ,Ion ,chemistry.chemical_compound ,Drinking water ,Detection limit ,Wax ,Prussian blue ,Chemistry ,010401 analytical chemistry ,Repeatability ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Pre-concentration ,Reagent ,visual_art ,Silver ion ,visual_art.visual_art_medium ,0210 nano-technology ,Pre concentration ,Wax printing - Abstract
In this work, a wax-patterned chromatographic paper has been utilized as a holistic platform to 1) synthesize Prussian Blue Nanoparticles (sensing species), 2) load the reagents for the assay, 3) concentrate the sample through multistep, and 4) visualize the determination of silver ions. Waters are continuously affected by changes in the composition, thus the utilization of reagent-free analytical tools is of huge interest for smart drinking water monitoring. Herein, we report the characterization and application of a multi-array paper-based platform for the colorimetric determination of silver ions based on the conversion from Prussian Blue to its silver-based analogue, namely Ag4[Fe(CN)6]. In particular, the platform highlights the increase of sensitivity due to paper pre-concentration of sample, that can be easily adapted to the analytical necessities. Within the proposed experimental setup, Ag+ is visualized down to a detection limit of 0.9 μM, with high repeatability and satisfactory recoveries in the range comprised between 90 and 113%.
- Published
- 2021
39. Facile development of cost effective and greener for all solid-state supercapacitor on paper substrate
- Author
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Stefano Cinti, Cherif Dridi, Fabiana Arduini, Achref Chebil, Vincenzo Mazzaracchio, Chebil, A., Mazzaracchio, V., Cinti, S., Arduini, F., and Dridi, C.
- Subjects
Materials science ,020209 energy ,Energy Engineering and Power Technology ,02 engineering and technology ,engineering.material ,Energy storage ,Paper-based substrate ,chemistry.chemical_compound ,Settore CHIM/01 ,Coating ,Carbon black ,Gel electrolyte ,Graphite ink ,0202 electrical engineering, electronic engineering, information engineering ,Graphite ,Screen printing ,Electrical and Electronic Engineering ,Supercapacitor ,Prussian blue ,Nanocomposite ,Renewable Energy, Sustainability and the Environment ,021001 nanoscience & nanotechnology ,Flexible electronics ,Flexible solid state supercapacitor ,Chemical engineering ,chemistry ,engineering ,0210 nano-technology - Abstract
The introduction of paper-based platforms for developing novel energy storage devices such as supercapacitors (SCs) highlights new promising opportunities in the field of flexible electronics. Herein, the use of paper-based substrate has shown reduced manufacturing cost and simplified coating process by screen-printing technology, as well as an improvement of the multilayer structure adhesion. The SC manufactured with Graphite ink mixed with Carbon Black (CB)/Prussian blue (PB) at different weight ratios (0, 3, 4, 5, 7, and 10 wt %) shows good performances. An optimum weight ratio of carbon black/prussian blue. 4 wt % is consistent with the following features: i) specific capacitance of 253 mF/cm² at 0.01 V/s, ii) specific energy density of 0.5 mWh/cm², iii) specific power density of 0.1 mW/cm², and iv) good cycling stability (94%) after 5000 cycles. The proposed fabrication approach exhibits a simple scale-up, a low environmental impact and a decrease of manufacturing costs: it provides self-supporting electrodes based on a mixture of graphite ink and CB/PB nanocomposite.
- Published
- 2021
40. Paper-based synthesis of Prussian Blue Nanoparticles for the development of whole blood glucose electrochemical biosensor
- Author
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Danila Moscone, Fabiana Arduini, Roberto Cusenza, Stefano Cinti, Cinti, S., Cusenza, R., Moscone, D., and Arduini, F.
- Subjects
Blood Glucose ,Paper ,Surface Properties ,Reducing agent ,Paper-based ,Electrode ,Surface Propertie ,Nanoparticle ,Nanotechnology ,Biosensing Techniques ,02 engineering and technology ,01 natural sciences ,Analytical Chemistry ,Biosensing Technique ,chemistry.chemical_compound ,Settore CHIM/01 - Chimica Analitica ,Glucose oxidase ,Particle Size ,Hydrogen peroxide ,Electrodes ,Prussian blue ,Electrochemical Technique ,biology ,Filter paper ,010401 analytical chemistry ,Electrochemical Techniques ,Hydrogen-Ion Concentration ,021001 nanoscience & nanotechnology ,BiosensorPaper-basedPoint-of-carePrussian Blue NanoparticlesScreen-printed electrodesWhole blood ,Environmentally friendly ,0104 chemical sciences ,Whole blood ,Prussian Blue Nanoparticle ,chemistry ,Point-of-care ,Screen-printed electrode ,biology.protein ,Nanoparticles ,0210 nano-technology ,Biosensor ,Ferrocyanide ,Ferrocyanides - Abstract
Nowadays, environmentally friendly synthesis pathways for preserving the environment and minimizing waste are strongly required. Herein, we propose filter paper as a convenient scaffold for chemical reactions. To demonstrate this novel approach, Prussian Blue Nanoparticles (PBNPs) were synthesized on filter paper by utilizing few μL of its precursors without external inputs, i.e. pH, voltage, reducing agents, and without producing waste as well. The functional paper, named “Paper Blue”, is successfully applied in the sensing field, exploiting the reduction of hydrogen peroxide at low applied potential. The eco-designed “Paper Blue” was combined with wax- and screen-printing to manufacture a reagentless electrochemical point-of-care device for diabetes self-monitoring, by using glucose oxidase as the biological recognition element. Blood glucose was linearly detected for a wide concentration range up to 25 mM (450 mg/dL), demonstrating its suitability for management of diabetes and glucose-related diseases. The Paper Blue-based biosensor demonstrated a correlation coefficient of 0.987 with commercial glucose strips (Bayer Contour XT). The achieved results demonstrated the effectiveness of this approach, which is also extendible to other (bio)systems to be applied in catalysis, remediation, and diagnostics.
- Published
- 2018
41. Carbon black as an outstanding and affordable nanomaterial for electrochemical (bio)sensor design
- Author
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Vincenzo Mazzaracchio, Stefano Cinti, Danila Moscone, Aziz Amine, Viviana Scognamiglio, Fabiana Arduini, Arduini, F., Cinti, S., Mazzaracchio, V., Scognamiglio, V., Amine, A., and Moscone, D.
- Subjects
Paper ,Immunosensors ,DNA sensors ,Materials science ,Nanostructure ,Electrode ,Microfluidics ,Biomedical Engineering ,Biophysics ,DNA sensor ,Nanotechnology ,Enzymatic biosensors ,Biosensing Techniques ,02 engineering and technology ,Immunosensor ,01 natural sciences ,Paper-based biosensor ,Nanomaterials ,Carbon-based nanomaterial ,Biosensing Technique ,Settore CHIM/01 ,Soot ,Carbon based nanomaterials ,Electrochemistry ,Animals ,Humans ,Paper-based biosensors ,Electrodes ,Electrochemical Technique ,Animal ,010401 analytical chemistry ,Electrochemical Techniques ,General Medicine ,Carbon black ,Equipment Design ,021001 nanoscience & nanotechnology ,Nanostructures ,0104 chemical sciences ,Carbon-based nanomaterials ,Enzymatic biosensor ,Bio sensor ,0210 nano-technology ,Biosensor ,Biotechnology ,Human - Abstract
Advances in cutting-edge technologies including nanotechnology, micmfluidics, electronic engineering, and material science have boosted a new era in the design of robust and sensitive biosensors. In recent years, carbon black has been re-discovered in the design of electrochemical (bio)sensors thanks to its interesting electroanalytical properties, absence of treatment requirement, cost-effectiveness (c.a. 1 (sic)/Kg), and easiness in the preparation of stable dispersions. Herein, we present an overview of the literature on carbon black-based electrochemical (bio)sensors, highlighting current trends and possible challenges to this rapidly developing area, with a special focus on the fabrication of carbon black-based electrodes in the realisation of sensors and biosensors (e.g. enzymatic, immunosensors, and DNA-based).
- Published
- 2020
42. Paper-based electrochemical peptide nucleic acid (PNA) biosensor for detection of miRNA-492: a pancreatic ductal adenocarcinoma biomarker
- Author
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Danila Moscone, Stefano Cinti, Concetta Avitabile, Maria Moccia, Veronica Caratelli, Fabiana Arduini, Anna Lisa Imbriani, Biagio Pede, Michele Saviano, Moccia, M., Caratelli, V., Cinti, S., Pede, B., Avitabile, C., Saviano, M., Imbriani, A. L., Moscone, D., and Arduini, F.
- Subjects
Peptide Nucleic Acids ,Serum ,Pancreatic ductal adenocarcinoma ,Biomedical Engineering ,Biophysics ,02 engineering and technology ,Biosensing Techniques ,Adenocarcinoma ,01 natural sciences ,chemistry.chemical_compound ,Settore CHIM/01 ,microRNA ,Ruthenium (III) hexamine ,Electrochemistry ,medicine ,Humans ,Screen-printing ,Peptide nucleic acid ,010401 analytical chemistry ,General Medicine ,Electrochemical Techniques ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Electrochemical gas sensor ,MicroRNAs ,Wax-printing ,medicine.anatomical_structure ,chemistry ,Duplex (building) ,Cancer research ,Differential pulse voltammetry ,0210 nano-technology ,Pancreas ,Biosensor ,Biomarkers ,Biotechnology - Abstract
Pancreatic ductal adenocarcinoma is the predominant neoplastic disease of the pancreas and it represents the fourth most frequent cause of death in cancer-related disease, with only 8% of survivors after 5-year to the diagnosis. The main issues of this type of cancer rely on fast progress (i.e. 14 months from T1 to a T4 stage), nonspecific symptoms with delay in diagnosis, and the absence of effective screening strategies. To address the lack of early diagnosis, we report a cost-effective paper-based biosensor for the detection of miRNA-492, which is recognised as a biomarker for pancreatic ductal adenocarcinoma. To design a miniaturised, sensitive, and robust paper-based platform, an electrochemical sensor was screen-printed on office paper previously wax-patterned via wax-printing technique. The paper-based sensor was then engineered with a novel and highly specific peptide nucleic acid (PNA) as the recognition element. The formation of PNA/miRNA-492 adduct was evaluated by monitoring the interaction between the positively charged ruthenium (III) hexamine with uncharged PNA and/or negatively charged PNA/miRNA-492 duplex by differential pulse voltammetry. The paper-based biosensor provided a linear range up to 100 nM, with a LOD of 6 nM. Excellent selectivity towards one- and two-base mismatches (1MM, 2MM) or scrambled (SCR) sequences was highlighted and the applicability for biomedical analyses was demonstrated, measuring miRNA-492 in undiluted serum samples.
- Published
- 2020
43. Multi-potential biomarkers for seafood quality assessment: global wide implication for human health monitoring
- Author
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Amina Antonacci, Amit Kumar Sinha, Vincenzo Mazzaracchio, Albana Uka, Stefano Cinti, Vikas Kumar, Fabiana Arduini, Viviana Scognamiglio, Kumar, V., Sinha, A. K., Uka, A., Antonacci, A., Scognamiglio, V., Mazzaracchio, V., Cinti, S., and Arduini, F.
- Subjects
seafood quality ,integumentary system ,Quality assessment ,media_common.quotation_subject ,010401 analytical chemistry ,Seafood industry ,food and beverages ,biosensors ,01 natural sciences ,Hazard ,0104 chemical sciences ,Analytical Chemistry ,Consumer satisfaction ,Human health ,Settore CHIM/01 ,Potential biomarkers ,Chemical contaminants ,Quality (business) ,Business ,Environmental planning ,nanomaterials ,Spectroscopy ,media_common - Abstract
Seafood products are widely accepted for their high nutritional properties. However, their safety should be comprehensively monitored, because chemical contaminants/xenobiotics may accumulate in seafood at levels that can pose a potential human health hazard. Indeed, seafood is one of the most vulnerable and perishable products, so its quality assessment is a prime concern throughout the production process, from harvesting, post-harvest microbial contaminations, post-mortem changes, processing, storage up to the consumption. Rapid monitoring of seafood quality and safety through very early biomarkers can bring considerable benefits to seafood industry and consumer satisfaction. A variety of analytical technologies for identifying a set of biomarkers to map (and improve) seafood production, quality, and safety, are discussed spanning from high-throughput proteomics to frontline biosensing strategies, both devoted to identify biomarkers for seafood management and assess the impact of water borne contaminants and pathogens on fish/shellfish welfare.
- Published
- 2020
44. Experimental Comparison in Sensing Breast Cancer Mutations by Signal ON and Signal OFF Paper-Based Electroanalytical Strips
- Author
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Emily P. Nguyen, Fabiana Arduini, Claudio Parolo, Giulia Cinotti, Danila Moscone, Stefano Cinti, Arben Merkoçi, Veronica Caratelli, Cinti, S., Cinotti, G., Parolo, C., Nguyen, E. P., Caratelli, V., Moscone, D., Arduini, F., and Merkoci, A.
- Subjects
Paper ,DNA, Single-Stranded ,Breast Neoplasms ,STRIPS ,Biosensing Techniques ,010402 general chemistry ,computer.software_genre ,01 natural sciences ,Signal ,Field (computer science) ,Analytical Chemistry ,law.invention ,Biosensing Technique ,DNA-based biosensors ,Breast cancer ,Settore CHIM/01 ,Design and Development ,law ,Experimental comparison ,Detection methods ,medicine ,Humans ,Liquid biopsy ,Protocol (science) ,Electrochemical Technique ,Chemistry ,010401 analytical chemistry ,Analytical performance ,Electrochemical Techniques ,medicine.disease ,Signal on ,0104 chemical sciences ,Emerging technologies ,Mutation ,Single strand DNA ,Female ,Data mining ,Detection protocols ,Biosensor ,computer ,Breast Neoplasm ,Human - Abstract
Altres ajuts: the ICN2 is funded by the CERCA Programme/Generalitat de Catalunya. The development of paper-based electroanalytical strips as powerful diagnostic tools has gained a lot of attention within the sensor community. In particular, the detection of nucleic acids in complex matrices represents a trending topic, especially when focused toward the development of emerging technologies, such as liquid biopsy. DNA-based biosensors have been largely applied in this direction, and currently, there are two main approaches based on target/probe hybridization reported in the literature, namely Signal ON and Signal OFF. In this technical note, the two approaches are evaluated in combination with paper-based electrodes, using a single strand DNA relative to H1047R (A3140G) missense mutation in exon 20 in breast cancer as the model target. A detailed comparison among the analytical performances, detection protocol, and cost associated with the two systems is provided, highlighting the advantages and drawbacks depending on the application. The present work is aimed to a wide audience, particularly for those in the field of point-of-care, and it is intended to provide the know-how to manage with the design and development stages, and to optimize the platform for the sensing of nucleic acids using a paper-based detection method.
- Published
- 2019
45. A challenge in biosensors: Is it better to measure a photon or an electron for ultrasensitive detection?
- Author
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Patrizia Simoni, Martina Zangheri, Elisa Marchegiani, Laura Fabiani, Aldo Roda, Noemi Colozza, Danila Moscone, Mara Mirasoli, Fabiana Arduini, Roda A., Arduini F., Mirasoli M., Zangheri M., Fabiani L., Colozza N., Marchegiani E., Simoni P., and Moscone D.
- Subjects
Chemiluminescence ,Biomedical Engineering ,Biophysics ,Amperometry ,Reproducibility of Result ,Nanotechnology ,Electrons ,02 engineering and technology ,Biosensing Techniques ,Immunosensor ,Electron ,01 natural sciences ,Horseradish peroxidase ,Sensitivity and Specificity ,law.invention ,Biosensing Technique ,Settore CHIM/01 ,law ,Electrochemistry ,Electrochemical biosensor ,Enzyme-based biosensor ,Photons ,Electrochemical Technique ,biology ,Chemistry ,Paper-based assay ,010401 analytical chemistry ,Reproducibility of Results ,General Medicine ,Electrochemical Techniques ,021001 nanoscience & nanotechnology ,Photon ,0104 chemical sciences ,Luminescent Measurement ,Luminescent Measurements ,biology.protein ,0210 nano-technology ,Biosensor ,Biotechnology - Abstract
Biosensor development exploiting various transduction principles is characterized by a strong competition to reach high detectability, portability and robustness. Nevertheless, a literature-based comparison is not possible, as different conditions are employed in each paper. Herein, we aim at evaluating which measurement, photons or electrons, yields better biosensor performance. Upon outlining an update in recent achievements to boost analytical performance, amperometry and chemiluminescence (CL)-based biosensors are directly compared employing the same biospecific reagents and analytical formats. Horseradish peroxidase (HRP) and hydrogen peroxide concentrations were directly measured, while glucose and mouse IgG were detected employing an enzyme paper-based biosensor and an immunosensor, respectively. Detectability was down to picomoles of hydrogen peroxide (4 pmol for CL and 210 pmol for amperometry) and zeptomoles of HRP (45 zmol for CL and 20 zmol for amperometry); IgG was detected down to 12 fM (CL) and 120 fM (amperometry), while glucose down to 17 μM (CL) and 40 μM (amperometry). Results showed that amperometric and CL biosensors offered similar detectability and analytical performance, with some peculiarities that suggest complementary application fields. As they generally provided slightly higher detectability and wider dynamic ranges, CL-based biosensors appear more suitable for point-of-care testing of clinical biomarkers, where detectability is crucial. Nevertheless, as high detectability in CL biosensors usually requires longer acquisition times, their rapidity will allocate electrochemical biosensors in real-time monitoring and wearable biosensors. The analytical challenge demonstrated that these biosensors have competitive and similar performance, and between photons and electrons the competition is still open.
- Published
- 2019
46. A 96-well wax printed Prussian Blue paper for the visual determination of cholinesterase activity in human serum
- Author
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Fabiana Arduini, Danila Moscone, Renato Massoud, Mohammad Saraji, Stefano Cinti, Neda Bagheri, Veronica Caratelli, Bagheri, N., Cinti, S., Caratelli, V., Massoud, R., Saraji, M., Moscone, D., and Arduini, F.
- Subjects
Paper ,Computer science ,Biomedical Engineering ,Biophysics ,Biosensing Techniques ,02 engineering and technology ,01 natural sciences ,Biosensing Technique ,chemistry.chemical_compound ,Limit of Detection ,Activity detection ,Electrochemistry ,Humans ,Settore CHIM/01 - Chimica Analitica ,Paper-based assay, Colorimetric detection, Multiplexed measurement, Butyrylcholinesterase, Chromatography paper ,Waxe ,Coloring Agent ,Coloring Agents ,Chromatography paper ,Detection limit ,Prussian blue ,Chromatography ,Paper-based assay ,010401 analytical chemistry ,Equipment Design ,General Medicine ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Thiocholine ,chemistry ,Waxes ,Butyrylcholinesterase ,Printing, Three-Dimensional ,Colorimetry ,0210 nano-technology ,Multiplexed measurement ,Ferrocyanide ,Colorimetric detection ,Human ,Ferrocyanides ,Biotechnology - Abstract
In the last decades, there is a growing search for analytical strategies to ensure clinical analysis without the need of laboratory set-up and skilled personnel. Indeed, user-friendly and low-cost devices are highly valued in the era of sustainability for their capability to be applied in low-resource contexts, such as developing countries. To address this issue, herein we report a 96-well paper-based and laboratory setup-free optical platform for the detection of butyrylcholinesterase enzyme (BChE) activity in human serum. We used chromatographic paper to realize a novel analytical tool exploiting its porous structure for reagentless synthesize Prussian Blue Nanoparticles (the sensing element), as well to load all the reagents required for the measurement. The principle of BChE activity detection relies on the reaction between the enzymatic product thiocholine and Prussian Blue, giving the Prussian White with subsequently Prussian Blue's fading, detected by a common office scanner supported by ImageJ software. Using this novel paper-based optical platform, BChE activity was linearly detected in the 2–15 U/mL range with a detection limit down to 0.8 U/mL. The accuracy was successfully demonstrated by recovery study with spiked serum and by comparing the data with the gold standard method.
- Published
- 2019
47. Origami multiple paper-based electrochemical biosensors for pesticide detection
- Author
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Luca Amendola, Stefano Cinti, Danila Moscone, Fabiana Arduini, Veronica Caratelli, Giuseppe Palleschi, Arduini, F., Cinti, S., Caratelli, V., Amendola, L., Palleschi, G., and Moscone, D.
- Subjects
Paper ,Insecticides ,Biomedical Engineering ,Biophysics ,02 engineering and technology ,Biosensing Techniques ,Standard solution ,Butyrylcholinesterase Alkaline phosphatase Tyrosinase Paraoxon 2,4-dichlorophenoxyacetic acid Atrazine ,01 natural sciences ,Paraoxon ,chemistry.chemical_compound ,Organophosphorus Compounds ,Rivers ,Limit of Detection ,Alkaline phosphatase ,Electrochemistry ,medicine ,Humans ,Settore CHIM/01 - Chimica Analitica ,Atrazine ,Pesticides ,Insecticide ,River ,Prussian blue ,Chromatography ,2,4-dichlorophenoxyacetic acid ,Filter paper ,010401 analytical chemistry ,Water ,General Medicine ,Pesticide ,021001 nanoscience & nanotechnology ,Potentiostat ,0104 chemical sciences ,chemistry ,Butyrylcholinesterase ,Tyrosinase ,Organophosphorus Compound ,0210 nano-technology ,Biosensor ,Butyrylcholinesterase Alkaline phosphatase Tyrosinase Paraoxon 2 ,4-dichlorophenoxyacetic acid Atrazine ,Water Pollutants, Chemical ,Biotechnology ,medicine.drug ,Human - Abstract
Herein, we propose the first three-dimensional origami paper-based device for the detection of several classes of pesticides by combining different enzyme-inhibition biosensors. This device was developed by integrating two different office paper-based screen-printed electrodes and multiple filter paper-based pads to load enzymes and enzymatic substrates. The versatile analysis of different pesticides was carried by folding and unfolding the filter paper-based structure, without any addition of reagents and any sample treatment (i.e. dilution, filtration, pH adjustment). The paper-based platform was employed to detect paraoxon, 2,4-dichlorophenoxyacetic acid, and atrazine by exploiting the capability of these different types of pesticides (i.e. organophosphorus insecticides, phenoxy-acid herbicides, and triazine herbicide) to inhibit butyrylcholinesterase, alkaline phosphatase, and tyrosinase, respectively. The degree of inhibition correlating to the quantity of pesticides was evaluated by chronoamperometrically monitoring the enzymatic activity in the absence and in the presence of pesticides by using a portable potentiostat. To improve the sensitivity, the paper-based electrodes were modified with carbon black alone in the case of platforms for 2,4-dichlorophenoxyacetic acid and atrazine detection, or decorated with Prussian blue nanoparticles for the detection of paraoxon. The paper-based device was applied for the detection of paraoxon, 2,4-dichlorophenoxyacetic acid, and atrazine at ppb level in both standard solutions and river water sample. The accuracy of this origami multiple paper-based electrochemical biosensor was evaluated in river water by recovery studies, obtaining satisfactory values (e.g. for paraoxon 90 ± 1% and 88 ± 2%, for 10 and 20 ppb, respectively). The proposed three-dimensional origami paper device allows for rapid, cost-effective and accurate pesticide detection in surface water as a result of combining filter and office papers, screen-printing, wax-printing and nanomaterial technology.
- Published
- 2019
48. Paper-Based Strips for the Electrochemical Detection of Single and Double Stranded DNA
- Author
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Danila Moscone, Federica Casotto, Stefano Cinti, Elena Proietti, Fabiana Arduini, Cinti, S., Proietti, E., Casotto, F., Moscone, D., and Arduini, F.
- Subjects
Paper ,Electrode ,Reproducibility of Result ,DNA, Single-Stranded ,Metal Nanoparticles ,Nanotechnology ,STRIPS ,010402 general chemistry ,Electrochemistry ,01 natural sciences ,Analytical Chemistry ,law.invention ,chemistry.chemical_compound ,Metal Nanoparticle ,law ,Settore CHIM/01 - Chimica Analitica ,Electrodes ,Kinetic ,Filter paper ,Electrochemical Technique ,Oligonucleotide ,010401 analytical chemistry ,Solid Phase Extraction ,HIV ,Reproducibility of Results ,DNA ,Electrochemical Techniques ,0104 chemical sciences ,Kinetics ,chemistry ,Colloidal gold ,Costs and Cost Analysi ,Costs and Cost Analysis ,Gold ,Double stranded - Abstract
The detection of double stranded DNA (dsDNA) is often associated with the use of laboratory-bound approaches and/or with the prior generation of single stranded DNA (ssDNA), making these methods not suitable for in situ monitoring, i.e., point-of-care diagnostics. Screen-printed technology, coupled to the use of triplex forming oligonucleotides (TFO) as the recognizing probes, offers a great possibility toward the development of portable analytical tools. Moreover, the continuous demand for sustainable processes and waste lowering have highlighted the role of paper-based substrates for manufacturing easy-to-use, low-cost, and sustainable electrochemical devices. In this work, filter paper and copy paper have been utilized to produce E-DNA strips. Gold nanoparticles (AuNPs) have been exploited to immobilize the methylene blue (MB)-tagged TFO and to enhance the charge transfer kinetics at the electrode surface. Both paper-based substrates have been electrochemically characterized, and in addition, the effect of the amount of waxed layers has been evaluated. The paper-based E-DNA strips have been challenged toward the detection of three model targets, obtaining 3 and 7 nM as the detection limit, respectively, for single and double stranded sequences. The repeatability of the manufacturing (homemade) process has been evaluated with a relative standard deviation of approximately 10%. The effectiveness of the filter paper-based platform has been also evaluated in undiluted serum obtaining a similar value of the detection limit (compared to the measurements carried out in buffer solution). In addition, a synthetic PCR amplified dsDNA sequence, related to HIV, has been detected in serum samples.
- Published
- 2018
49. Magnetic carbon spheres and their derivatives combined with printed electrochemical sensors
- Author
-
Manuela Scarselli, Francesca Limosani, Fabiana Arduini, Stefano Cinti, Cinti, S., Limosani, F., Scarselli, M., and Arduini, F.
- Subjects
Materials science ,Magnetic carbon structure ,Scanning electron microscope ,General Chemical Engineering ,Smart sampling ,chemistry.chemical_element ,02 engineering and technology ,Chemical vapor deposition ,010402 general chemistry ,Electrochemistry ,01 natural sciences ,symbols.namesake ,Settore CHIM/01 - Chimica Analitica ,Spectroscopy ,Magnetic carbon structuresScreen-printed electrodesSmart sampling ,021001 nanoscience & nanotechnology ,Ascorbic acid ,0104 chemical sciences ,Chemical engineering ,chemistry ,Screen-printed electrode ,Electrode ,symbols ,0210 nano-technology ,Raman spectroscopy ,Carbon - Abstract
Herein, we report the synthesis of several magnetic carbon structures starting from spheres (CSs) to more elongated structures to get tubes and fibers by customizing chemical vapour deposition parameters. These CSs-based products, ranging from nano-to micro-size, were investigated by morphological point of view using scanning electron microscopy, energy dispersive x-ray spectroscopy, and Raman spectroscopy. These carbon structures were combined with screen-printed electrodes highlighting their electrochemical effectiveness towards the detection of several species, i.e. ferricyanide, ascorbic acid, dopamine, cysteine, serotonin, and NADH. The presence of iron nuclei within the carbonaceous lattices, besides having improved the electrochemical performances at the printed electrodes, might confer these CSs-based structures a future application in the field of remediation/sensing. Herein, some preliminary applications were provided showing that these materials can be easily employed to collect species and, successively, coupled with printed electrochemical sensors.
- Published
- 2018
50. Novel bio-lab-on-a-tip for electrochemical glucose sensing in commercial beverages
- Author
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Danila Moscone, Fabiana Arduini, Vincenzo Mazzaracchio, Stefano Cinti, Roberta Marrone, Cinti, S., Marrone, R., Mazzaracchio, V., Moscone, D., and Arduini, F.
- Subjects
Materials science ,Biomedical Engineering ,Biophysics ,Lab-on-a-tip ,Nanotechnology ,Biosensing Techniques ,02 engineering and technology ,01 natural sciences ,Beverages ,Matrix (chemical analysis) ,Glucose Oxidase ,Electroanalysi ,Settore CHIM/01 ,Electrochemistry ,Miniaturization ,Glucose oxidase ,Beverage ,Electrodes ,Detection limit ,biology ,010401 analytical chemistry ,Pipette ,Electrochemical Techniques ,General Medicine ,Chronoamperometry ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Glucose ,Filter (video) ,biology.protein ,0210 nano-technology ,Biosensor ,Biotechnology - Abstract
The development of portable and user-friendly sensing platforms is a hot topic in the field of analytical chemistry. Among others, electroanalytical approaches exhibit a high amenability for reaching this purpose, i.e. the commercial strips for diabetes care are an obvious success. However, providing fully-integrated and reagent-free methods is always a leitmotiv. In this work, we evaluated the use of a disposable pipette tip, opportunely configured to demonstrate the first example of an electrochemical biosystem in a pipette tip, namely bio-lab-on-a-tip. The combination of a pipette tip, wire electrodes, enzyme, and cotton wool filter, allows the fabrication of a novel electroanalytical platform that does not need expertise-required tasks. To demonstrate the feasibility of this novel method, glucose is detected in beverages by means of chronoamperometry. The experimental setup, entirely built inside the pipette tip, is able to 1) block impurities/interferences from matrix, 2) load/release reagents for the bio-assay, 3) reduce the operating task to zero, and 4) perform electrochemical detection. With optimized experimental parameters, the bio-lab-on-a-tip is able to detect glucose linearly up to 10 mM with a detection limit of 170 μM. The effectiveness of the platform was confirmed by testing commercial beverages, e.g. Coca-Cola and Coca-Cola Zero, with high accuracy. In addition, the shelf-life of the novel device was evaluated, highlighting the role of cotton wool filter for providing a suitable environment for glucose oxidase stability. The novel concept can be easily generalized for further applications in the field of non-invasive clinical diagnostics and in-situ environmental monitoring.
- Published
- 2020
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