1. Monoclonal antibodies to the main immunogenic region of the nicotinic acetylcholine receptor bind to residues 61-76 of the alpha subunit
- Author
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B. Roth, M. Ballivet, G.J. Hughes, S.J. Tzartos, Alex Mauron, C. Alliod, T. Barkas, and J.M. Gabriel
- Subjects
medicine.drug_class ,Recombinant Fusion Proteins ,Molecular Sequence Data ,Receptors, Nicotinic ,Recombinant fusion proteins immunology ,Biology ,Torpedo ,Monoclonal antibody ,Biochemistry ,law.invention ,Amino acid sequence ,Epitopes immunology ,Epitopes ,Mice ,Electrophoresis polyacrylamide gel ,Receptors nicotinic immunology ,law ,Molecular sequence data ,Antibodies, monoclonal immunology ,medicine ,Animals ,Amino Acid Sequence ,Peptide fragments immunology ,Binding site ,Binding sites antibody ,Molecular Biology ,G alpha subunit ,Acetylcholine receptor ,Immunoassay ,Antibodies, Monoclonal ,Cell Biology ,Molecular biology ,Fusion protein ,Peptide Fragments ,Rats ,Nicotinic acetylcholine receptor ,Electrophoresis, Polyacrylamide Gel ,Binding Sites, Antibody ,Chickens ,Alpha chain - Abstract
Monoclonal antibodies (mAbs) to the main immunogenic region (MIR) bind to fusion proteins containing region 37-200 of the alpha chain of Torpedo, mouse, and chicken nicotinic acetylcholine receptor. In the case of the mouse alpha chain, these mAbs react with sequence 61-216 but not with 74-216. A synthetic peptide M1, containing residues 61-76 of the mouse alpha chain, also binds these anti-MIR mAbs, showing that all or part of their binding site is included in this region. The conformational dependence and epitope specificity of the mAbs are discussed.
- Published
- 1988