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Supplemental material, sj-docx-1-smo-10.1177_20503121231175759 for Association between micronutrients, oxidative stress biomarkers and angiogenic growth mediators in early and late-onset preeclamptic Ghanaian women by Enoch Odame Anto, Wina Ivy Ofori Boadu, Otchere Addai-Mensah, Yaw Amo Wiafe, William KBA Owiredu, Christian Obirikorang, Max Efui Annani-Akollor, Eric Adua, Michael Appiah, Stephen Opoku, Emmanuel Acheampong, Evans Adu Asamoah, Eddie-Williams Owiredu, Agartha Odame Anto, Augustine Tawiah, Frank Ankobea, Ebenezer Afrifa Yamoah and David Antony Coall in SAGE Open Medicine

Supplemental material, sj-docx-1-smo-10.1177_20503121231175759 for Association between micronutrients, oxidative stress biomarkers and angiogenic growth mediators in early and late-onset preeclamptic Ghanaian women by Enoch Odame Anto, Wina Ivy Ofori Boadu, Otchere Addai-Mensah, Yaw Amo Wiafe, William KBA Owiredu, Christian Obirikorang, Max Efui Annani-Akollor, Eric Adua, Michael Appiah, Stephen Opoku, Emmanuel Acheampong, Evans Adu Asamoah, Eddie-Williams Owiredu, Agartha Odame Anto, Augustine Tawiah, Frank Ankobea, Ebenezer Afrifa Yamoah and David Antony Coall in SAGE Open Medicine

Additional file 1. Primers used, BLASTP hits for HRP2 sequence modelled from Ghanaian isolates and BLASTP of Ghanaian HRP2 sequences for all 50 samples.

Objectives. Sub-Saharan Africa (SSA) is observing an accelerating prevalence rate of type 2 diabetes mellitus (T2DM) influenced by gene-environment interaction of modifiable and nonmodifiable factors. We conducted a systematic review and meta-analysis on the heritability and genetic risk of T2DM in SSA. Methods. We reviewed all published articles on T2DM in SSA between January 2000 and December 2019 and available in PubMed, Scopus, and Web of Science. Studies that reported on the genetics and/or heritability of T2DM or indicators of glycaemia were included. Data extracted included the study design, records of family history, pattern and characteristics of inheritance, genetic determinants, and effects estimates. Results. The pattern and characteristics of T2DM heritability in SSA are preference for maternal aggregation, higher among first degree compared to second-degree relatives; early age-onset (

Glioma is the world’s commonest primary brain malignancy with much of its biology relating to translational and post-translational events still unknown. In this study, we investigated the clinicopathological significance of N-linked β1-6-GlcNAc branches and GnT-V enzyme in the development and progression of astrocytic glioma. Expression of GnT-V and its GlcNAc-β1-6 oligosaccharides by-product together with Con-A binding sugars were assessed immunohistochemically on tissue microarrays of 16 normal brain and 159 tissue samples of astrocytomas of variable grades and histology. Although tissues of both grade I astrocytomas and normal brain showed considerably higher GnT-V expression, GlcNAc-β1-6 expression was significantly high only in tissues of grade I astrocytomas (p < 0.001), which is attributable to elevated levels of the precursor Con-A binding sugar moieties (p < 0.001). The activity of GnT-V enzyme was found to be dependent on the degree of glioma pathogenesis, as the GlcNAc-β1-6 branched expression diminished with every progressive grade of glioma, reaching minimum in glioblastoma (p < 0.001). Having biphasic activity in gliomagenesis, the role of GnT-V in glioma was deciphered by generating different ectopic GnT-V expressions in U-87 cells, which showed the highest GnT-V expression among selected glioma cell lines. Transient GnT-V rescue was achieved in knockdown clones by transfection with GnT-V expression vector. Suppression of GnT-V in U-87 cells slowed cell proliferation with G0/G1 cell cycle phase arrest. Reduced tumorigenicity, invasiveness and cell-ECM interactions were also associated with suppressed in vitro GnT-V activity suggesting GnT-V may act as an oncoprotein. We report for the first time that GnT-V products are involved in early gliomagenesis but their reduced expression, correlating with low Con-A binding sugars level found in high tumor grades predicts the loss of total N-glycosylation in glioma development and may be of potential diagnostic and/or prognostic value in astrocytoma.