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8 results on '"Anglade, I."'

1. Effect of (xeno)-estrogens on zebrafish P450c17 (C17, 17 alpha-hydroxylase/17, 20-lyase) mRNA and protein expression in gonadal tissue

Author

Brion, F., Hinfray, N., Palluel, O., Maillard, J., Anglade, I., Aït-Aïssa, S., Olivier, K. A. H., Jean-Marc PORCHER, Institut National de l'Environnement Industriel et des Risques (INERIS), Interactions Cellulaires et Moléculaires, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-IFR98-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-IFR98-Centre National de la Recherche Scientifique (CNRS), Interactions cellulaires et moléculaires (ICM), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Civs, Gestionnaire, and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDE] Environmental Sciences, GENE EXPRESSION, endocrine system, [SDV.TOX.ECO] Life Sciences [q-bio]/Toxicology/Ecotoxicology, GENE PROTEINE EXPRESSSION, P450C17, ZEBRAFISH, urogenital system, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, GONADES, GONADS, POISSON ZEBRE---STEROIDOGENESIS, Gene expression, Gonads, P450c17, Steroidogenesis, Zebrafish, [SDE]Environmental Sciences, ComputingMethodologies_GENERAL, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, STEROIDOGENESE, ComputingMilieux_MISCELLANEOUS
Abstract

International audience; The objectives of this study were to determine the expression and the cellular localization of P450c17 in gonadal tissues in the zebrafish and to investigate the effect of (xeno)-hormones on its expression at both mRNA and protein levels. We showed that gonads are sites for cyp17 gene expression and we documented for the first time the cellular localization of the cyp17 protein expression within ovarian and testicular tissues in a cyprinid fish. Moreover, we demonstrated the down-regulating effect of (xeno)-estrogens on testicular cyp17 expression.

Published
2008
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3. Cloning, tissue distribution, and central expression of the gonadotropin-releasing hormone receptor in the rainbow trout (Oncorhynchus mykiss)

Author

Madigou, T., Mananos-Sanchez, E., Hulshof, S., Anglade, I., Silvia Zanuy, Kah, O., Centre National de la Recherche Scientifique (France), Ministère de la Recherche et des Technologies (France), Institut National de la Recherche Agronomique (France), Fondation Daniel Langlois pour l'Art, la Science et la Technologie, and European Commission

Subjects
endocrine system, Central nervous system, GnRH, GnRH receptor, Anterior pituitary
Abstract

A full-length cDNA encoding a GnRH receptor (GnRH-R) has been obtained from the brain of rainbow trout. This cDNA encodes a protein of 386 amino acids (aa) exhibiting the typical arrangement of the G-protein-coupled receptors in seven transmembrane domains. However, a second ATG could give rise to a receptor with a 30-aa longer extracellular domain. As already shown in other fish and Xenopus, this protein possesses an intracellular domain, in contrast with its mammalian counterparts. In the case of rainbow trout, this intracellular carboxy-terminal tail consists of 58 residues. Northern blotting experiments carried out in the brain, the pituitary, and the liver only resulted in a single band of 1.9-2 kilobases in the pituitary, although reverse transcription-polymerase chain reaction amplification products were found in the brain, the pituitary, the retina, and the ovary. In situ hybridization using a probe corresponding to the full-length coding region of the receptor was performed on vitellogenic or ovulating females and allowed to detect a weak but specific signal in the proximal pars distalis of the pituitary, the preoptic region, the mediobasal hypothalamus, and the optic tectum. However, the strongest signal was consistently detected in a mesencephalic structure, the nucleus lateralis valvulae, the significance of which is presently open to speculation., Supported by the CNRS, INRA, Ministère de la Recherche et de la Technologie, Fondation Langlois, and European Union (Fair CT97-3785). The first two authors have contributed equally to this study.

Published
2000

4. Localization of nuclear androgen receptor messengers in the brain and pituitary of the rainbow trout

Author

Menuet, Arnaud, Guiguen, Yann, Teitsma, C., Pakdel, Farzad, Mananos, E., Mazurais, D., Kah, O., Anglade, I., UPRES-A 6026 Endocrinologie moléculaire de la reproduction, Centre National de la Recherche Scientifique (CNRS), Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES), Université de Rennes (UR), and ProdInra, Migration

Subjects
[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, [SDV.BDLR] Life Sciences [q-bio]/Reproductive Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
1999

5. Involvement of gamma-aminobutyric acid in the control of GHT-1 and GHT-2 secretion in male and female rainbow trout

Author

Mananos, E.L., Anglade, I., Chyb, J., Saligaut, Christian, Breton, Bernard, Kah, Olivier, Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], GABA, [SDV]Life Sciences [q-bio], SALMONIDAE ONCORHYNCHUS MYKISS, [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
1999

8. Comparaison de la distribution de GnRH de mammifère (Hormone fournissant de la Gonadotropine) et de GnRH II de poulet dans le cerveau du jeune esturgeon sibérien (Acipenser baeri)

Author

Lepretre, E., Anglade, I., Williot, Patrick, Vandesande, F., Tramu, G., Kah, Olivier, Irstea Publications, Migration, Aquaculture et pêche (UR APBX), Centre national du machinisme agricole, du génie rural, des eaux et forêts (CEMAGREF), LABORATOIRE DE NEUROCYTOCHIMIE FONCTIONNELLE TALENCE, Partenaires IRSTEA, Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), and LABORATORY OF NEUROENDOCRINOLOGY LEUVEN BEL

Subjects
[SDE] Environmental Sciences, CEMAGREF, [SDE]Environmental Sciences
Abstract

The brain of the sturgeon has recently been shown to contain at least two forms of GnRH : mammalian GnRH and chicken GnRH-II. In this study, we compared the distribution of immunoreactive mGnRH and cGnRH-II in the brain of immature Siberian sturgeons., Il a été récemment démontré que le cerveau de l'esturgeon contenait au moins deux formes de GnRH : la GnRH de mammifère et la GnRH-II de poulet. Dans cette étude, nous avons comparé la répartition de la GnRH de mammifère et de la GnRH-II de poulet dans le cerveau de jeunes esturgeons de Sibérie.

Published
1993

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