Search

The aim of this research is to determine the effect of Althaea rosea flower gum loaded with Thymbra spicata essential oils coating on packaged beef patties during cold storage. For this purpose, samples were evaluated in terms of pH, color, thiobarbituric acid reactive substances (TBARS), and microbiological properties. In addition, texture profile analysis (TPA) was performed to evaluate the textural properties of the beef patties. The essential oil treatment to the beef patties had a significant effect (p

Pestil is one of the important foods traditionally produced in Türkiye, with high energy, vitamin and mineral content. It is obtained by naturally drying the pulp obtained from different kinds of fruits after mixing it with ingredients such as sugar and flour. In regions where the fruiting period depends on the season, fruit pulp is produced in order to benefit from it in winter. In addition, bioactive components originating from the fruit increase the nutritional value. In this study, the physicochemical and antioxidative properties of the pestil obtained from three different Hawthorn fruits (Crataegus meyeri, Crataegus turkestenica and Crataegus orientalis) known to have positive effects on health were investigated. The results revealed that, depending on the fruit type, pestil samples have high antioxidative properties (IC50: 50.11±0.16-52.1±2.14 mg/mL) due to the high phenol content (39.8±0.16-52.95±1.21 mg GA/g DW). In this context, in the present study, it has been seen that the pestil prepared with the traditional method from three different Hawthorn fruits should be industrially produced as a product for people to access healthy and various foods today.

In Turkish cuisine, ready–to–eat vegetable salads (REVS) served with pide/lahmacun, kebab types, and tantuni from animal source in meat restaurants were evaluated since they have the potential to carry risks in terms of Public Health. The microbiological properties of REVS were investigated using agar plate method. Antimicrobial resistance of foodborne pathogens including Escherichia coli and Staphylococcus aureus was tested using Kirby–Bauer disc diffusion method. Moreover, the presence of important enteric viruses was detected by Polymerase Chain Reaction (PCR). The number of total aerobic bacteria, coliform bacteria, yeast and molds and, Staphylococcus and Micrococcus spp. ranged from less than 1 to 6.40, 1 to 6.26, less than 1–5.82 and less than 1–5.66 log10 colony forming units·grams-1 (CFU·g–1) in REVS samples, respectively. None of the REVS tested in this study contained Salmonella spp., whereas E. coli and S. aureus were isolated in 38.1% (16/42) and 2.4% (1/42), respectively. S. aureus was resistant to gentamicin, kanamycin, aztreonam, and ciprofloxacin in the disc diffusion assay, however, it was not harboring the mecA gene. E. coli strains (n=16) were resistant (100%) to aminoglycoside antibiotics and 35.7% (6/16) of the isolates were extended spectrum beta lactamase (ESBL) producing. blaTEM and blaCTXM8/25 were detected in two isolates, whereas one isolate carried blaCTXM–1 and blaTEM together by PCR. Of the REVS, two were evaluated as positive for rotavirus (4.8%), six for hepatitis A (14%), and hepatitis E virus (14%). These results indicate the high microorganism load, presence of ESBL E. coli, and viral enteric pathogens in REVS, hence it is important to perform routine hygiene practices.

Since the middle of the twentieth century, the use of dyes has become more common in every food group as well as in the pharmaceutical, textile and cosmetic industries. Azo dyes, including carmoisine, are the most important of the dye classes with the widest color range. In this study, the effects of carmoisine exposure on the embryonic development of zebrafish at a wide dose scale, including recommended and overexposure doses (from 4 to 2000 ppm), were investigated in detail. For this purpose, many morphological and physiological parameters were examined in zebrafish exposed to carmoisine at determined doses for 96 h, and the mechanisms of action of the changes in these parameters were tried to be clarified with the metabolite levels determined. The no observed effect concentration (NOEC) and median lethal concentration (LC

Fluoride exposure through drinking water, foods, cosmetics, and drugs causes genotoxic effects, oxidative damage, and impaired cognitive abilities. In our study, the effects of fluoride on anxiety caused by the circadian clock and circadian clock changes in a zebrafish model were investigated at the molecular level on parents and the next generations. For this purpose, adult zebrafish were exposed to 1.5 ppm, 5 ppm, and 100 ppm fluoride for 6 weeks. At the end of exposure, anxiety-like behaviors and sleep/wake behaviors of the parent fish were evaluated with the circadian rhythm test and the novel tank test. In addition, antioxidant enzyme activities and melatonin levels in brain tissues were measured. In addition, morphological, physiological, molecular and behavioral analyzes of offspring taken from zebrafish exposed to fluoride were performed. In addition, histopathological analyzes were made in the brain tissues of both adult zebrafish and offspring, and the damage caused by fluoride was determined. The levels of BMAL1, CLOCK, PER2, GNAT2, BDNF and CRH proteins were measured by immunohistochemical analysis and significant changes in their levels were determined in the F

Salmonella spp. are zoonotic pathogenic agents that cause important infections in humans and animals. They are the most common foodborne pathogens after Campylobacter spp. worldwide. The aim of this study was to determine the phenotypic and genotypic extended-spectrum β-lactamase (ESBL) and colistin resistance of 67 Salmonella spp. isolated from retail chicken meats between May and December 2016, and stored in the culture collection of Ataturk University, Faculty of Veterinary Medicine, Department of Microbiology. The isolates were serotyped using multiplex polymerase chain reaction (mPCR). The serovar distribution of strains was 74.6% S. Infantis, 16.4% S. Enteritidis, 3.0% S. Arizonae, 3.0% S. Dublin, 1.5% S. Gallinarum and 1.5% S. Indica. Of the 67 strains, 20 (29.9%) were ESBL-positive. The main types of β-lactamase identified were blaCTX-M-1, blaCTX-M-8-25, blaSHV and blaTEM. Four isolates were found to be phenotypically colistin-resistant. These isolates did not carry mobilised colistin resistance (mcr) genes 1 to 5. In this study, both genotypically ESBL-producing and phenotypically colistin-resistant Salmonella strains were found. We revealed that ESBL-producing Salmonella strains have dramatically increased over the years, especially when compared with previously reported chicken meat Salmonella strains in Turkey. The increase in Salmonella strains, particularly ESBL producers and the colistin resistant, is of great concern for selected antimicrobial therapy in human infections. Hence, epidemiological information and monitoring systems are extremely important in controlling Salmonella infections in public health services.

Anxiety and obesity are two current phenomena. They are among the important public health problems with increasing prevalence worldwide. Although it is claimed that there are strong relations between them, the mechanism of this relationship has not been fully clarified yet. On the other hand, the effect of this relationship on the offspring has been another research subject. In this study, obese zebrafish were obtained by feeding two different diets, one containing high amount of lipid (HF) and the other containing high amount of carbohydrate (HK), and their anxiety levels were evaluated. To establish a relationship between these two phenomena, in addition to histopathological and immunohistochemical analysis in the brain tissues of fish, the transcription levels of some genes related to lipid and carbohydrate metabolisms were determined. In addition, offspring were taken from obese zebrafish and studied to examine the effect of parental obesity on offspring. As a result, it was observed that the HC diet, causing more weight increase than the HF diet, showed an anxiolytic while the HF diet an anxiogenic effect. It was suggested that the probable cause of this situation may be the regulatory effect on the appetite-related genes depending on the upregulation severity of the PPAR gene family based on the diet content. In addition, it was also suggested that it may have contributed to this process in neuron degenerations caused by oxidative stress. Regarding effects on offspring, it can be concluded that HF diet-induced obesity has more negative effects on the next generation than the HC diet. Level of evidence No Level of evidence: animal study.

Colistin is one of the most effective antibiotics against multidrug resistant Gram-negative bacteria. However, the recent emergence of plasmid-borne mobilized colistin resistance (mcr) genes is con...

Global warming further increases the toxic threat of environmental pollutants on organisms. In order to reveal the dimensions of this threat more clearly, it is of great importance that the studies be carried out with temperature differences as close as possible to the temperature values that will represent the global climate projection. In our study, how the toxicity of glyphosate, which is widely used around the world, on zebrafish changes with temperature increases of 0.5° was investigated on behavioral and molecular basis. For this purpose, adult zebrafish were exposed to glyphosate at concentrations of 1 ppm and 5 ppm for 96 h in four environments with a temperature difference of 0.5° (28.5; 29.0; 29.5; 30.0 °C). At the end of the exposure, half of the zebrafish were sampled and remaining half were left for a 10-day recovery process. At the end of the trials, zebrafish were subjected to circadian rhythm and anxiety tests. In addition, histopathological, immunohistochemical and metabolome analyses were performed on brain tissues. As a result, it has been detected that anxiety and circadian rhythm were disrupted in parallel with the increased temperature and glyphosate concentration, and increased histopathological findings and 5-HT4R and GNAT2 immunopositivity in the brain. As a result of metabolome analysis, more than thirty annotated metabolites have been determined due to the synergistic effect of temperature increase and glyphosate exposure. As a conclusion, it was concluded that even a temperature increase of 0.5° caused an increasing effect of glyphosate toxicity in the zebrafish model.

Ready-to-eat (RTE) salads service and utilize with pide/lahmacun, kebab types, and tantuni in meat restaurants in Turkish cuisine. Hence, the vegetable salads served with meat-based RTE foods are a risk factor for public health. In this study, we report the microbiological properties, antimicrobial resistance of foodborne pathogens including E. coli and S. aureus, and the presence of important enteric viruses in eastern Turkey. The total aerobic bacteria (TAB) ranged from − 1 in the RTE salads (n = 42), whereas yeast and molds was ranged from − 1. The coliform bacteria and the Staphylococcus and Micrococcus spp. count were determined − 1 and − 1 in all REVS samples, respectively. None of the RTE vegetable salads (REVS) tested in this study contained Salmonella spp., whereas E. coli and S. aureus were isolated in 38.1% (16/42) and 2.4% (1/42), respectively. S. aureus was resistant to gentamicin, kanamycin, aztreonam, and ciprofloxacin in the disc diffusion assay, however, it was not harboring the mecA gene. E. coli strains (n = 16) were resistant (100%) to aminoglycoside antibiotics (gentamicin and kanamycin) and 35.7% (6/16) of the isolates were ESBL producing. BlaTEM and blaCTXM8/25 were detected in two isolates, whereas one isolate carried blaCTXM−1 and blaTEM together by PCR. Of the REVS, two were evaluated as positive for rotavirus (4.8%), and six for hepatitis A (14.3%) and hepatitis E virus (14.3%). These results indicate the high microorganism load, presence of ESBL E. coli, and viral enteric pathogens in REVS, hence it is important to perform routine hygiene practices.

Antioxidant substances are used not only in food and beverages, but also in soap formulations which are known to be effective in skin cleansing, and are also used as ingredients in the detergent and cosmetics market. The aim of this study was to determine the bactericidal effects of plain and antibacterial soaps containing Punica granatum flower (PGF), Carrot seed (CS, Daucus carota), Lavender (LV, Lavandula officinalis L.) extracts which are known for their high antioxidant activity. Antioxidant properties were determined by using the cupric ion reducing antioxidant capacity (CUPRAC) and Ferric reducing antioxidant power (FRAP) for all soap samples. The effectiveness of soaps which are prepared using PGF, CS and LV extracts on gram positive and gram negative bacteria was investigated. From the findings obtained, all plant extracted soaps had antibacterial activity and it was determined that soap containing carrot extract caused a significant decrease in bacterial levels compared to other soaps. As a result, it has been concluded that the use of antimicrobial and antioxidant compounds as soap formulation components is extremely important for increasing the effectiveness of soap against bacteria.

The aim of this study was to investigate the effects of two modified semen diluents on the spermatological parameters of Saanen goat sperm stored at 4°C. Ejaculates were obtained from five Saanen bucks by means of an electro-ejaculator and divided into five aliquots. Then, aliquots of ejaculates were used to create five experimental groups as follows: Group 1 (control), group 2A containing seminal plasma (SP) and bovine serum albumin (BSA; 10 mg/mL-1), group 2B containing BSA without SP, group 3A containing egg yolk (EY; 17.00%) and SP and finally group 3B containing EY without SP. All aliquots of semen were extended with Tris-based extender. Spermatological parameters of experimental groups were observed at 0, 6, 12 and 24 h periods during storage at 4°C. Motility of semen in groups 2A and 2B was found to be best values compared to other groups. However, the motility of group 3A at 24 h was 0.00%. In conclusion, it was concluded that the addition of 10 mg/mL-1 of BSA to Tris-based extender could be useful in the storage of Saanen goat semen regardless of SP at 4°C.

Nano-sized plastic particles formed from both daily use plastics and its debris have become a potential health and environmental problem due to features such as transportation through food webs and maternal transfer. Although many studies on the toxicity of plastics exist more detailed and molecular studies are needed to evaluate and review the effects of plastics especially in nano-size range. For this purpose, we have microinjected polystyrene nanoplastics (PNP) (20 nm) to the zebrafish embryo, which is one of the best model organisms for developmental toxicity studies, to simulate intake with food or maternal. Survival, hatching and malformations evaluated during the experimental period (120 h). Moreover, we have aimed to put forth the presence of reactive oxygen species (ROS) and apoptosis signalling accumulation in the body in addition to bioaccumulation of PNP and immunochemical toxicity (8-OHdG) on the brain of zebrafish larvae at the 120th hour. According to results, it has been demonstrated that 20 nm diameter PNP can reach the brain and bioaccumulate there, moreover lead to oxidative DNA damage in the brain regions where it bioaccumulates. Here we have also imaged the PNP from a vertebrate brain via transmission electron microscopy (TEM) for the first time. As a result of these, it has been detected increasing mortality and prevailing abnormalities in addition to excessive ROS and apoptosis in especially the brain. As a conclusion, obtained data have suggested that precautions, on the use and contamination of the plastic product, to be taken during both pregnancy and baby care/feeding are important for the health of the baby in future.

Fungi that contaminate the fruits at stages such as ripening, harvesting, storage, transportation may cause deterioration and economic losses, and even some strains can produce mycotoxins known to be harmful to health. In this respect, it was examined that the mold diversity of some fruits sold in eastern Turkey. A total of 113 different fruits, both non-moldy and moldy, were collected and 395 strains were isolated and identified using classical methods from different parts of the fruits (surfaces, core cavities and rotten parts). It was found the 11 different genera of fungi including Penicillium spp. (34.43%), Cladosporium spp. (22.53%), Rhizopus spp. (21.01%), Alternaria spp. (8.10%), Botrytis spp. (7.34%), Aspergillus spp. (2.27%), Byssochlamys spp. (1.52%), Acremonium spp. (0.76%), Fusarium spp. (0.76%), Colletotrichum spp. (0.76%), and Geotrichum spp. (0.51%) in the analysed strawberry, grape, apple, cherry, pear, plum, pomegranate, apricot, peach, orange, tangerine, and quince fruits. It is noteworthy that the fungi known to produce mycotoxins such as Penicillium expansum and Penicillium italicum are among the fungi isolated within the scope of the study.

Staphylococcus aureus is an opportunistic and ubiquitous pathogen found in the skin, nares, and mucosal membranes of mammals. Increasing resistance to antimicrobials including methicillin has become an important public concern. One hundred and eight (108) S. aureus strains isolated from a total of 572 clinical and animal products samples, were investigated for their biofilm capability, methicillin resistance, enterotoxin genes, and genetic diversity. Although only one strain isolated from raw retail was found as a strong biofilm producer, the percentage of antimicrobial resistance pattern was relatively higher. 17.59% of S. aureus strains tested in this study were resistant to cefoxitin and identified as methicillin-resistant S. aureus (MRSA) isolates. mecA and mecC harboring S. aureus strains were detected at a rate of 2.79% and 0.93%, respectively. In addition, staphylococcal enterotoxin genes including Sea, Seb, Sec, and Sed genes were found to be 18.5%, 32.4%, 6.5% and 3.7%, respectively. The phylogenetic relationship among the isolates showed relationship between joint calf and cow milk isolates. Multi locus sequence typing (MLST) revealed three different sequence types (STs) including ST84, ST829, and ST6238. These findings highlight the development and spread of MRSA strains with zoonotic potential in animals and the food chain throughout the world.

The increase in temperature due to global warming greatly affects the toxicity produced by pesticides in the aquatic ecosystem. Studies investigating the effects of such environmental stress factors on next generations are important in terms of the sustainability of ecosystems. In this study, the effects of parental synergistic exposure to glyphosate and temperature increase on the next generation were investigated in a zebrafish model. For this purpose, adult zebrafish were exposed to 1 ppm and 5 ppm glyphosate for 96 h at four different temperatures (28.5, 29.0, 29.5, 30.0 °C). At the end of this period, some of the fish were subjected to the recovery process for 10 days. At the end of both treatments, a new generation was taken from the fish and morphological, physiological, molecular and behavioral analysis were performed on the offspring. According to the results, in parallel with the 0.5-degree temperature increase applied to the parents with glyphosate exposure, lower survival rate, delay in hatching, increased body malformations and lower blood flow and heart rate were detected in the offspring. In addition, according to the results of whole mouth larva staining, increased apoptosis, free oxygen radical formation and lipid accumulation were detected in the offspring. Moreover, it has been observed that the temperature increases to which the parents are exposed affects the light signal transmission and serotonin pathways in the offspring, resulting in more dark/light locomotor activity and increased thigmotaxis.

Global warming and plastic pollution are among the most important environmental problems today. Unfortunately, our world is warming more than expected and biological life, especially in the oceans, has come to the limit of the struggle for survival with the nano-scale plastic pollution that is constantly released from the main material. In this study, the synergic effect of one-degree temperature increase (28, 29, 30 °C) and 100 nm size polystyrene plastic nanoparticles on circadian rhythm, brain damage and metabolomics in zebrafish were investigated in an environment where temperature control with 0.05-degree precision is provided. A temperature increase of 1°, together with nanoplastic exposure, affected the circadian rhythm in zebrafish, caused damage to the brain and caused significant changes in the intensity of a total of 18 metabolites in different pathways. It was also detected Raman signals of polystyrene in the brain homogenate. As a consequence, it is suggested that one degree of temperature increase pave the way for degeneration in the brain by disrupting some metabolic pathways, thereby significantly increasing the negative effects of nano-plastic on behavior.

With the development of sequencing technology, microRNAs (miRNA) have between 19-24 bases in length which play a role in cell communication was discovered. These miRNAs have important tasks in the development and differentiation of cells, as well as in metabolism. The miRNAs carried in the microvesicle are prevented from degradation depending on the conditions such as extreme pH and temperature. In the current studies, it has been determined that the foods have regulate the activities at cellular level in metabolism by miRNA expression. ’This was first demonstrated by detecting rice miR-168a which absorbed by digestive system. On the other hand, these miRNAs were found to inhibit the mRNA expression of LDL RAP1 involved in cholesterol transport. In studies where miRNAs have been absorbed by the digestive system, it has been suggested that miRNAs, in particular animal origin, pass into serum and plasma. With the miRNAs have this potential, it can be used as biological markers for the prevention of the health problems associated with diet. It’s known that foods contain the components such as fat, sugar, protein, vitamin affect to metabolism not only, but also miRNAs affect it directly or indirectly. A recent dietary miRNA database was created with the increasing importance of foodborne miRNAs and there are miRNA profiles associated with foods such as apples, Atlantic salmon, bananas, chicken meat and egg, corn, beef fat and milk, breast milk, grapes, oranges, pork, rice, tomato, soybeans and wheat in this database. In this study, biosynthesis, the mechanism of action and presence in food and effects on metabolism of miRNAs were discussed.

Nanotechnological approaches are the latest modality for early stage detection of cancer. The need of rapid, non-invasive, patient specific, and informative techniques in cancer diagnostics lead to the utilization of nanotechnology, microfluidics, and lab-on-a-chip platforms for liquid biopsy, and the developments through these technologies increased the knowledge also for case specific applications. In this review, nanotechnology-based methodologies that are developed in the last decade for cancer diagnostics are investigated and are discussed under four main categories for the purpose of simplification as; nanochip based, nanofilm based, magnetic nanomaterial-based methods, and combinational utilization of multiple methodologies. We suggest a combinational approach on device development with an aim of producing a compact, cost effective, rapid, sensitive, and non-invasive diagnostic device as a conclusion of literature review.

With the ever-increasing plastic pollution, the nano-sized plastic particles that are constantly released from the main materials have a greater potential threat. Studies continue on how to eliminate plastic waste, which has become a global problem, from nature. We are aware that complete elimination is not easy at all, but it is not known clearly that even if it is successful, its effects on organisms will also disappear completely. In this study, zebrafish injected with 20 nm-sized polystyrene particles (PS) only during the embryonic period were grown in an environment without plastic exposure. The effects of PS on their offspring embryo/larvae were examined at morphological, molecular and metabolomic levels. Results showed that parental PNP exposure caused significant malformations, decreased survival rate, increased heart rate and blood flow rate, as well as decreased eye size, height and locomotor activity, which were attributed to growth retardation in the offspring. According to the results of whole-mount immunofluorescence larval staining, cell death and reactive oxygen species were significantly increased, while lipid accumulation was decreased in new generation larvae from zebrafish injected with PNP. In order to elucidate the mechanisms underlying these morphological, physiological and molecular damages, the metabolome analyses were performed by evaluating the Q-TOF MS/MS spectra with chemometric analyses in the offspring larvae. According to the metabolomics results, 28 annotated metabolomes suggested by the OPLS-DA analysis that may vary significantly through a variable in projection scores were detected. In addition, it was detected that the significantly increased histopathological findings and immunopositivity of JNK, H2A.X, PI3 and NOP10 in new generation larvae. In conclusion, it has been shown that exposure to PS, even only during the embryonic period, may affect many cancer-related biological processes in the next generation.

Microfluidic systems are relatively new technology field with a constant need of novel and practical manufacturing materials and methods. One of the main shortcomings of current methods is the inability to provide rapid bonding, with high bonding strength, and sound microchannel integrity. Herein we propose a novel method of assembly that overcomes the mentioned limitations. Polymer-assisted bonding is a novel, rapid, simple, and inexpensive method where a polymer is solubilized in a solvent and the constituted solution is used as a bonding agent. In this study, we combined this method with utilization of several phase-changing materials (PCMs) as channel-protective agents. Glauber’s salt appeared to be more suitable as a channel-protective agent compared to rest of the salts that have been used in this study. Based on the bonding strength, quality analyses, leakage tests, and SEM imaging, the superior assisting bonding solvent was determined to be dichloromethane with a PMMA concentration of 2.5% (W/V). It showed a bonding strength of 23.794 MPa and a nearly non-visible bonding layer formation of 2.83 µm in width which is proved by SEM imaging. The said combination of PCM, solvent, and polymer concentration also showed success in leakage tests and an application of micro-droplet generator fabrication. The application was carried out to test the applicability of developed prototyping methodology, which resulted in conclusive outcomes as the droplet generator simulation run in COMSOL Multiphysics version 5.1 software. In conclusion, the developed fabrication method promises simple, rapid, and strong bonding with sharp and clear micro-channel engraving.

The effect of sunflower oil (SO), sunflower oil-pomegranate sauce (PS1) and sunflower oil-plum sauce (PS2) on shelf life of marinated carp fillets was investigated in terms of sensory evaluation, chemical (peroxide value (POV), thiobarbituric acid (TBA), free fatty acids (FFA), total volatile basic nitrogen (TVB-N) and pH), and microbiological properties (Total viable count (TVC), Escherichia coli, Total coliform, Staphylococci/Micrococci, Salmonella spp.) during 30 days of storage. Proximate composition, which includes total crude protein%, fat%, moisture% and ash% of fresh carp fillets before marination process was analyzed and found 18.69±0.86, 4.08±0.19, 74.33±0.63 and 2.17±0.45, respectively. Sensory evaluation analysis showed that total appearance, odor-taste and texture scores decreased during storage. TVB-N significantly increased in all group after 1 month of storage (P

Ethyl carbamate (EC, urethane), which is used as an anesthetic especially by veterinarians due to its very long duration of action, is also a naturally occurring compound in all fermented foods and beverages. Although the health problem of EC is related to its carcinogenic potential, the scarcity of current studies that can be used in the evaluation of usage limits encouraged us to do this study. In this context, zebrafish embryos were exposed to serial doses of EC. According to the results, it was observed that EC exposure caused a significant decrease in survival and hatching rates as well as significant body malformations. Whole-mount staining results showed that EC caused dose-dependent increased apoptosis. Oxidative stress caused by EC exposure was demonstrated by whole-mount staining, transcriptional and immunohistochemically. Furthermore, it has been shown histochemically that EC exposure causes necrosis and degeneration in the brain. In behavioral tests, it was observed that EC caused hyperactivity associated with these neuronal degenerations. In addition, a dramatic decrease in blood flow was detected in association with pericardial edema. In the light of the current results, it should be carefully considered that EC can be found naturally in many human diets, especially fermented foods.

Salmonella spp. are zoonotic pathogenic agents that cause important infections in humans and animals. They are the most common foodborne pathogens after Campylobacter spp. worldwide. The aim of this study was to determine the phenotypic and genotypic extended-spectrum β-lactamase (ESBL) and colistin resistance of 67 Salmonella spp. isolated from retail chicken meats between May and December 2016, and stored in the culture collection of Ataturk University, Faculty of Veterinary Medicine, Department of Microbiology. The isolates were serotyped using multiplex polymerase chain reaction (mPCR). The serovar distribution of strains was 74.6% S. Infantis, 16.4% S. Enteritidis, 3.0% S. Arizonae, 3.0% S. Dublin, 1.5% S. Gallinarum and 1.5% S. Indica. Of the 67 strains, 20 (29.9%) were ESBL- positive. The main types of β-lactamase identified were blaCTX-M-1, blaCTX-M-8-25, blaSHV and blaTEM. Four isolates were found to be phenotypically colistin-resistant. These isolates did not carry mobilised colistin resistance (mcr) genes 1 to 5. In this study, both genotypically ESBL-producing and phenotypically colistin-resistant Salmonella strains were found. We revealed that ESBL-producing Salmonella strains have dramatically increased over the years, especially when compared with previously reported chicken meat Salmonella strains in Turkey. The increase in Salmonella strains, particularly ESBL producers and the colistin resistant, is of great concern for selected antimicrobial therapy in human infections. Hence, epidemiological information and monitoring systems are extremely important in controlling Salmonella infections in public health services., Salmonella spp. zoonotski su patogeni koji uzrokuju infekcije u ljudi i životinja. Na globalnoj su razini najčešći patogeni koji se prenose hranom poslije bakterija Campylobacter spp. Cilj ovog istraživanja bio je fenotipski i genotipski odrediti β-laktamaze proširenog spektra (ESBL) i otpornost na kolistin 67 sojeva bakterija Salmonella spp. izoliranih iz pilećeg mesa za prodaju od svibnja do prosinca 2016. i pohranjenih u kulturama na Odsjeku za mikrobiologiju Fakulteta veterinarske medicine na Sveučilištu Ataturk. Izolati su serotipizirani primjenom višestruke lančane reakcije polimerazom (mPCR). Distribucija serovara bila je: 74,6 % za S. Infantis, 16,4 % za S. Enteritidis, 3,0 % za S. Arizonae, 3,0 % za S. Dublin, 1,5 % za S. Gallinarum i 1,5 % za S. Indica. Od 67 sojeva njih je 20 (29,9 %) bilo pozitivno na ESBL. Glavni identificirani tipovi β-laktamaza bili su: blaCTX-M-1, blaCTX-M-8-25, blaSHV i blaTEM. Četiri izolata bila su fenotipski otporna na kolistin. Ovi izolati nisu sadržavali aktivne gene rezistencije na kolistin (mcr) 1 do 5. U ovom su istraživanju nađeni i sojevi salmonele koji su genotipski proizvodili ESBL i sojevi fenotipski otporni na kolistin. Sojevi salmonele koji proizvode ESBL s godinama su se znatno povećali, posebno u usporedbi s prethodnim izvješćima o sojevima salmonele u mesu pilića u Turskoj. Porast sojeva salmonele, osobito onih koji proizvode ESBL i onih koji su otporni na kolistin, zabrinjava zbog antimikrobnog liječenja infekcija u ljudi. Zbog toga su u kontroli infekcija salmonelama u javnome zdravstvu iznimno važni epidemiološki podaci i sustavi praćenja.

Butylated hydroxyanisole (BHA) has been widely used in the cosmetics, pharmaceutical, and food industries due to its antioxidant activity. Despite the antioxidant effects, reported adverse effects of BHA at the cellular level have made its use controversial. In this regard, this study was performed to elucidate the potential toxicity mechanism caused by BHA at the molecular level in zebrafish embryos. For this purpose, zebrafish embryos were exposed to BHA at levels of 0.5, 1, 5, 7.5 and 10 ppm and monitored at 24, 48, 72 and 96 hours. Survival rate, hatching rate and malformations were evaluated. We examined the potential for reactive oxygen species (ROS) production and apoptosis signalling accumulation in the whole body. Moreover, we evaluated histopathological and immunohistochemical (8-OHDG) characterization of the brain in zebrafish embryos at the 96th hour. We also examined apoptosis, histopathological and immunohistochemical (8-OHDG) characteristics in 96 hpf zebrafish larvae exposed to tertiary butylhydroquinone (TBHQ), one of the major metabolites of BHA, at doses of 0.5, 2.5, 3.75 and 5 ppm. Consequently, it has been considered that increased embryonic and larval malformations in this study may have been caused by ROS-induced apoptosis. After 96 h of exposure, positive 8-OHdG immunofluorescence, degenerative changes, and necrosis were observed in the brain of BHA and TBHQ-treated zebrafish larvae in a dose-dependent manner. BHA and TBHQ exposure could lead to an increase in 8-OHdG activities by resulting oxidative DNA damage. In particular, the obtained data indicate that the induction of ROS formation, occurring during exposure to BHA and/or multiple hydroxyl groups, could be responsible for apoptosis.

Colistin is one of the most effective antibiotics against multidrug resistant Gram-negative bacteria. However, the recent emergence of plasmid-borne mobilized colistin resistance (

The demand for meat is constantly rising due to the increasing world population. Factors such as insufficient resources, ethical concerns, and animal welfare encourage the new studies to discover alternative meat sources. One of these alternatives is in vitro meat (IVM), which is produced in a laboratory condition. Since IVM is a fairly new and unknown alternative, studies are needed to find out consumers' perceptions and parameters that affect their perception. In this context, our current research was established to investigate general attitudes towards IVM and to examine whether some predictive factors have an impact on consumers' attitudes. The study was established using 80 male and 81 female students attending Atatürk University Erzurum Vocational School. All questions asked to the participants are presented on a 1 ± 5 likert type scale. If the participants stated that they would not try IVM, other follow-up questions were not submitted. Then, all participants were given a versatile option, which investigated under what conditions they would not want to try IVM. Statistical analysis shows that students are somehow willing to consume IVM. On the other hand, it has been determined that taste/appearance is the primary obstacle in terms of IVM consumption. At the same time, it has been determined that gender and meat type are the most important variables in attitude towards IVM. However, all the results show that there is a deep confusion about the perception of IVM. Although the study is small-scale, it has revealed that it is an important step in measuring the perceptions of IVM and the need for further research on the subject of IVM., Artan dünya nüfusu nedeniyle ete olan talep günümüzde sürekli olarak artmaktadır. Mevcut kaynakların yetersiz oluşu, etik kaygılar, hayvan refahı gibi faktörler alternatif et kaynaklarının keşfi konusunda yeni çalışmalar yapmaya teşvik etmektedir. Bu alternatiflerden biri de temelde bir laboratuar ortamında üretilen in vitro ettir (IVE). IVE oldukça yeni ve bilinmeyen bir alternatif olduğundan, tüketicilerin ürüne karşı algılarının ve algılarını etkileyen parametrelerin ortaya konulmasıyla ilgili çalışmalara ihtiyaç duyulmaktadır. Bu bağlamda, mevcut araştırmamız, IVE’ye karşı genel tutumları araştırmak ve tüketicilerin tutumları üzerinde bazı öngörücü faktörlerin bir etkisi olup olmadığını incelemek için kurulmuştur. Çalışma Atatürk Üniversitesi Erzurum Meslek Yüksekokulunda okuyan 80 erkek ve 81 kadın öğrenci katılımcı kullanılarak kurulmuştur. Katılımcılara sorulan tüm sorular 1 ± 5 likert tipi ölçekte sunulmuştur. Katılımcılar IVE'yi denemeyeceklerini belirttiyse, diğer takip soruları sunulmamıştır. Daha sonra tüm katılımcılara, hangi koşullarda IVE'yi denemek istemeyeceklerini araştıran çok yönlü bir seçenek verilmiştir. İstatistiksel analizler, öğrencilerin bir şekilde IVE tüketmeye istekli olduklarını göstermektedir. Diğer taraftan IVE tüketimi konusunda tat/görünüşün öncelikli engel olduğu belirlenmiştir. Bununla birlikte, cinsiyet ve et türünün, IVE’ye yönelik tutumda en önemli değişkenler olduğu tespit edilmiştir. Ancak elde edilen tüm sonuçlar IVE algısı konusunda derin bir karmaşının olduğunu göstermektedir. Çalışma küçük ölçekli olsa da, IVE’ye yönelik algıların ölçülmesinde önemli bir adım olduğu ve IVE konusu hakkında daha fazla araştırmanın gerekliliğini ortaya koymuştur.

Patulin, bazi Penicillium, Aspergillus ve Byssochlamys kuf turleri tarafindan uretilen bir mikotoksindir. Bircok olumsuz saglik etkisi oldugu ve ticari meyve sularinda ve elma urunlerinde sik sik tespit edildigi gorulmustur ki bu da halk sagligi acisindan bir endise kaynagidir. Elma ve turevi urunlerde patulin bulasmasindan sorumlu en onemli organizma Penicillium expansum'dur (P. expansum). Elmalar / meyvelerde P. expansum buyumesinin inhibe edilmesi ve meyve urunlerinde patulin kontaminasyonunun azaltilmasi uzerine bircok calisma yapilmis olmasina ragmen, bu urunler dunyada hala buyuk bir kirlenme sorunu olmaya devam etmektedirler. Elma ve diger meyve kaynakli urunlerdeki patulin kirlenmesi cesitli fiziksel, kimyasal ve biyolojik kontrol yontemleri kullanilarak onlenebilir. Iyi Hasat Oncesi ve Hasat Sonrasi Uygulamalar gibi onleyici tedbirler, islem engelleriyle birlikte, kirlenmeyi onlemek icin en iyi cozum olabilir. Mevcut teknolojileri gelistirmek, yeni teknolojiler gelistirmek ve optimize etmek icin arastirma ve gelistirme de sorunu ortadan kaldirmak icin en iyi firsati verebilir.

The purpose of this study was to see if the microalgae species Chlorella miniata possess an antioxidant and antiinflammatorypotential and to see if there is a correlation between the cultivation conditions and these potentials. For this purpose, cultivation of the algae under different growth conditions had been carried out; afterwards the soxhletextraction of the produced biomass had been completed. Lastly the assays of Trolox equivalent antioxidant capacity (TEAC), ferric reducing antioxidant capacity (FARP), total antioxidant capacity (TAO), xanthine oxidase inhibition, and hyaluronidase inhibition were studied using extracts of microalgae obtained from the different cultivation parameters. It's important to mention that this was the first study using these assays on species of Chlorella miniata and the first that utilized the cultivation parameters on microalgae. The results were, 22,91 mg/g algae TEAC, 776,70 mg/g algae gallic acid equivalent of FRAP and 37,07% xanthine oxidase inhibition with a concentration of 10,5 mg algae per ml ethanol, 65,39% of hyaluronidase inhibition with a concentration of 0,9 mg algae per ml ethanol. The effects of the cultivation parameters on these assays and the Lc MS/MS analyses for various phenolic compounds in the extracts were also a part of the study.

Propyl gallate (PG) is a chemical compound obtained by esterification of propanol with gallic acid. Due to its antioxidative properties, it is widely used in cosmetics and pharmaceutical industries as well as to protect the oils in foods such as butter, milk-based desserts, chewing gum, mayonnaise, meat, soups, cereals, spices and seasonings from rancidity. This study has been designed to assessment 8-OHdG and 4-HNE activity, and histopathological changes in the brain tissues of zebrafish larvae, which is a lecithotrophic organism, after 96 h of PG exposure via microinjecting to yolk sac of embryo. To this end, approximately 5 nL of various concentrations of PG (1, 10, and 50 ppm) has been injected into yolk sac of fertilized embryo (final exposure concentrations are 5, 50, 250 pg/egg) with micro manipulator system. After 96 h exposure time, propyl gallate caused immunofluorescence positivity of 8-OHdG and 4-HNE in the brain tissues of zebrafish larvae. PG was not effect brain tissue histopathological in low concentrations (1 and 10 ppm) but highest concentration (50 ppm) caused degenerative changes in brain. These results suggests that PG treatment could lead oxidative DNA damage by causing an increase 8-OHdG and 4-HNE activities. This strategy will enable us to better understand the mechanisms of propyl gallate in brain tissues of zebrafish larvae.

The toxicity of sodiumcarboxymethyl cellulose (CMC), which has GRAS status and has been determined as "ADI non specified", was re-evaluated with a new modelling and molecular-based data. For this purpose, CMC, a food additive, was injected to the yolk sac (food) of the zebrafish embryo by the microinjection method at the 4th hour of fertilization at different concentrations. As a result, it was found that CMC showed no toxic effects within the framework of the parameters studied. But, we determined increasing lipid accumulation in zebrafish embryos exposed to CMC in a dose-dependent manner. To elucidate the mechanism underlying this lipid accumulation, the expression levels of genes related to obesity-linked lipid metabolism were examined. Our findings show that while CMC does not cause a toxic effect in zebrafish embryos, it can lead important effects on lipid metabolism by causing changes in the expression of some genes associated with obesity. (C) 2020 Elsevier B.V. All rights reserved.

Specific microbiological parameters and the presence of Salmonella spp. were investigated in 72 chicken meat samples (36 wings and 36 drumsticks) collected from markets and butcher shops. The specific microbiological parameters were determined using a conventional cultural method and the presence of Salmonella spp. in chicken samples was determined using conventional and immunomagnetic separation (IMS)-polymerase chain reaction (PCR) methods. In addition, antimicrobial susceptibility of the isolates was revealed using the Kirby-Bauer disc diffusion method. The results indicated that 30 of the 72 samples were positive for Salmonella spp. by the conventional method, and 42 of the 72 were positive by the IMS-PCR method. However, 30 of the 72 samples were positive for Salmonella spp. by both methods. The Salmonella spp. isolates were confirmed by the VITEK2 Compact System and PCR. The susceptibilities of the isolates against 10 antibiotics were determined. The results indicated that isolates (27/30) showed the highest susceptibility to gentamycin (90.00%), while the highest resistance was to nalidixic acid and tetracycline at the 100 and 93.34% levels, respectively. These results indicate a high prevalence of Salmonella spp. in poultry meat from Erzurum city, Turkey, and the antimicrobial resistance profile of these isolates should be considered for public health. The results also show that the IMS-PCR technique was superior to the conventional method for detecting Salmonella in poultry meat. Parâmetros microbiológicos específicos e a presença de Salmonella spp. foram investigados em 72 amostras de carne de frango (36 asas e 36 baquetas) coletadas em mercados e açougues. Os parâmetros microbiológicos específicos foram determinados utilizando um método cultural convencional e a presença de Salmonella spp. em amostras de frango foi determinada utilizando métodos de reação em cadeia da polimerase (PCR) por separação convencional e imunomagnética (IMS). Além disso, a suscetibilidade antimicrobiana dos isolados foi revelada pelo método de difusão do disco de Kirby-Bauer. Os resultados indicaram que 30 das 72 amostras foram positivas para Salmonella spp. pelo método convencional, e 42 das 72 foram positivas pelo método IMS-PCR. No entanto, 30 das 72 amostras foram positivas para Salmonella spp. por ambos os métodos. Os isolados de Salmonella spp. foram confirmados pelo sistema VITEK2 Compact e PCR. As susceptibilidades dos isolados a 10 antibióticos foram determinadas. Os resultados indicaram que os isolados (27/30) apresentaram maior suscetibilidade à gentamicina (90,00%), enquanto a maior resistência foi ao ácido nalidíxico e à tetraciclina nos níveis de 100 e 93,34%, respectivamente. Estes resultados indicam uma alta prevalência de Salmonella spp. em carne de frango da cidade de Erzurum, Turquia, e o perfil de resistência antimicrobiana desses isolados deve ser considerado para a saúde pública. Os resultados também demonstram que a técnica de IMS-PCR foi superior ao método convencional para detecção de Salmonella em carne de frango.

In this study, somephysicochemical and microbiological characteristics of 50 raw milk sampleswhich are sold in local dairy delicatessens in Erzurum province weredetermined. As a result of the research, the non-fat dry matter (%), fat (%),total dry matter (%), pH, acidity (%), and density values ​​of the milk samples were 7.6-10.5, 1.5-6.8, 10.9-15.1,6.46-7.33, 0.121-0.252 and 1.026-1.034, respectively. When the data wereevaluated according to the related standards, it was determined that 12% of theraw milk was not suitable for fat, 6% for non-fat dry matter and 12% for totalacidity. The total aerobic of mesophilic bacteria counts in 98% of the sampleswere > 100.000 cfu/mL-1, of the total coliform bacteria countswere in the range of 5.18-5.83 log cfu/mL-1 in 38%, the totalEnterococcus spp. counts were in the range of 5.76-6.68 log cfu/mL-1,the total number of Staphylococcus/Micrococcus spp. counts were in the range of3.60-4.80 log cfu/mL-1 in 60% and the yeast-mold counts 4.38-5.07log cfu/mL-1 in 36%. When all the results were taken intoconsideration, it was recognized that the samples were found to have worsemicrobiological quality than the chemical properties., Bu araştırmada Erzurumilinde lokal süt şarküterilerinde satışa sunulan 50 adet çiğ süt örneğinin bazıfizikokimyasal ve mikrobiyolojik özellikleri belirlenmeye çalışılmıştır.Araştırma sonucunda süt örneklerinin yağsız kurumadde (%), yağ (%), toplamkurumadde (%), pH, asitlik derecesi (%), ve yoğunluk değerlerinin sırasıyla7,6-10,5, 1,5-6,8, 10,9-15,1, 6,46-7,33, 0,121-0,252 ve 1,026-1,034 arasındadeğiştiği ve süt örneklerinin hepsinin karbonat testinin negatif olduğusaptanmıştır. Elde edilen veriler ilgili standartlara göre değerlendirildiğindeçiğ sütlerin %12’sinin yağ, %6’sının yağsız kurumadde, %12’sinin de asitlikdeğeri yönüyle uygun olmadığı saptanmıştır. Örneklerin, %98’inde toplammezofilik aerobik bakteri sayısının >100.000 kob/mL-1, %38’indetoplam koliform bakteri sayısının 5,18-5,83 log kob/mL-1 arasında,total Enterococcus türlerinin sayısının 5,76-6,68 log kob/mL-1arasında, toplam Staphylococcus/Micrococcus türlerinin sayısının % 60’ında3,60-4,80 log kob/mL-1 arasında ve maya-küf sayısının ise %36’sında4,38-5,.07 log kob/mL-1 arasında olduğu belirlenmiştir. Elde edilentüm sonuçlar göz önünde bulundurulduğunda incelenen örneklerin kimyasalözelliklerinden ziyade mikrobiyal kalitelerinin daha kötü olduğu tespitedilmiştir.

[No abstract available]

Antioxidant substances used at any stage of in vitro embryo production increase intracellular glutathione synthesis GSH improve nuclear maturation rates and protect embryos against endogenous or exogenous oxidative stresses making embryos resistant to freeze as well as increasing cell quality and number of embryos reaching blastocysts Production of GSH depends on the availability and uptake of cysteine in the medium However cysteine is very unstable outside the cell and is auto oxidized to cystine Cysteamine reduces cystine to cysteine and promotes the uptake of cysteine by cells thereby enhancing the GSH synthesis Consequently cysteamine plays an important role in the synthesis of GSH and is a key factor in the defense mechanism against ROS

Oxidative stress during in vitro culture leads to defects in development of gametes and embryos. Several antioxidants such as cysteamine, L-ascorbic acid, beta mercaptoethanol, cysteine, glutathione, proteins, vitamins have been used to supplement culture media to counter the oxidative stress. This study was conducted to detect the effect of adding cysteamine to the maturation medium to subsequent cleavage rates of sheep embryos. Totally 604 ovaries were obtained by ten replica and 2060 oocytes were collected. The cumulus oocyte complexes were recovered by the slicing method. A total of 1818 selected oocytes were divided into two groups and used for maturation (88.25%). The first group was created as supplemented with cysteamine (Group A) and second group (Group B, control) without cysteamine in TCM-199. The two groups were incubated for 24 h at 38.8 °C in an atmosphere of 5% CO2 in humidified air for in vitro maturation (IVM). After IVM, oocytes were fertilized with 50 x 107 / mL fresh ram semen in BSOF medium for 18 h. After fertilization, maturation groups were divided into two subgroups with different culture media: Group AI-SOF (Synthetic Oviduct Fluid medium), Group AII-CR1aa (Charles Rosencrans medium), Group BI-SOF and Group BII-CR1aa were achieved. Cleavage rates were evaluated at day 2. post insemination. The rates of cleavage were detected as 59.54% (184/309), 55.44% (173/312), 65.34% (215/329), 59.34% (200/337) respectively, with showing no statistically significant difference between the groups at the level of P>0.05. In conclusion, supplementing cysteamine to maturation media in TCM-199 did not affect the cleavage rates of sheep embryos in SOF and CR1aa culture media.

Propyl gallate (PG) is an antioxidant substance widely used in cosmetics, pharmaceutical and food industries. The aim of this study was to evaluate the potential toxic effect of PG injected to zebrafish embryos. To this end, zebrafish embryos were exposed to PG with 0, 1, 10 and 50 ppm concentrations which are lower than ADI and were monitored at 24, 48, 72 and 96 hpf. Survival rate, hatching rate and malformations were evaluated during this period. Moreover, it has been detected the accumulation of fluorescence signal of ROS and apoptotic cell in whole body at the end of 96 hpf. According to results, survival rate slightly decreased in highest concentration, and PG accelerated hatching in 1 and 10 ppm concentrations whereas delayed in 50 ppm concentration. In addition, it has been detected accumulation of fluorescence signal of ROS and apoptotic cells in a dose dependent-manner. Consequently, it has been considered that increased embryonic or larval malformation in this study may have been caused by ROS-induced apoptosis. The obtained data suggested that the developmental toxicity caused by PG and/or multiple hydroxyl groups arose when PG hydrolyze to gallic acid is probably triggered by the induction of ROS formation and consequent apoptosis.

Bu çalışmada bir mikro alg türü olan Chlorella miniata'nın baloncuk kolon tipi fotobiyoreaktörler içinde, BRISTOL ortamında ve seçilen ışık şiddetini veren beyaz floresan aydınlatma şartlarında, inkübasyon dolabında sabit şartlarda büyümesi incelenmiştir. Chlorella miniata ile 6 litrelik steril ortamlarında, (22°C, 245 µmol m-2s-1) sürekli bir stok biyokütle üretimi yapılmıştır. Üretim süreçleri 21 günlük bir büyüme evresi reaktör ortamından alınan günlük örneklerin analiz edilmesiyle kontrollü olarak tamamlanmıştır. Büyüme şartlarına bağlı olarak büyüme hızı sıcaklık, ışık şiddeti ve her ikisine de bağlı çeşitli modellerle incelenmiştir. Matematik modeller yardımıyla, uygun büyüme koşulları 21,38 oC sıcaklık ve 291,5 µmol foton m-2 s-1 olarak hesaplanmıştır. Biyokütleden Soxhlet ekstraksiyonu ile ekstraktlar elde edilmiş LC MS/MS çalışmaları sonucunda ekstraktların içeriğinde yüksek miktarlarda salisilik, kafeik vanilik, t-sinamik asit ve siyanidin ve mirisetin'e rastlanmıştır. Çalışma diğer bir amacı Chlorella miniata türünün olası antioksidan ve anti-inflamatuar kapasitesinin araştırılması ve bu olası kapasite ile yetiştirilme koşulları arasında bağlantılar aranmasıdır. Bu amaçla, trolox eşleniği olarak antioksidan kapasitenin ölçümü (TEAC), toplam antioksidan kapasitenin ölçümü (TAO), ferrik indirgen antioksidan kapasitenin ölçümü (FRAP), ksantin oksidaz inhibisyonu ve hiyaluronidaz inhibisyonu çalışmaları yetiştirilmiş mikro algler için yapılmıştır. Bu çalışmaların sonuçları, 22,91 mg/g alg TEAC, 776,70 mg/g alg gallik asit eşleniği FRAP ve 37,07% ksantin oksidaz inhibisyonu, 65,39% hiyaluronidaz inhibisyonu olarak bulunmuştur., The aim of the study was to acquire three findings regarding microalgae. The microalgae species Chlorella miniata was used for the study. The first step was to determine the specific growth rate changes of the microalgae with cultivation parameters, the experiments were carried out using varying cultivation conditions and they were performed using bubble column photobioreactors with a volume of 6 liters, and 1 vvm aeration, illuminated using white fluorescent light. Using mathematical modelling, the optimum temperature and light intensity for the maximum specific growth rate were calculated to be 21,38 oC and 291,5 µmol photon m-2 s-1 respectively. The last two parts of the study was to see if the microalgae species Chlorella miniata possess an antioxidant and anti-inflammatory potential and to see if there is a correlation between the cultivation conditions and these potentials. For this purpose the assays of Trolox equivalent antioxidant capacity (TEAC), ferric reducing antioxidant capacity (FARP), total antioxidant capacity (TAO), xanthine oxidase inhibition, and hyaluronidase inhibition were studied using extracts of microalgae obtained from the different cultivation parameters. It's important to mention that this was the first study using these assays on species of Chlorella miniata and the first that utilized the cultivation parameters on microalgae. The results were, 22,91 mg/g algae TEAC, 776,70 mg/g algae gallic acid equivalent of FRAP and 37,07% xanthine oxidase inhibition with a concentration of 10,5 mg algae per ml ethanol, 65,39% of hyaluronidase inhibition with a concentration of 0,9 mg algae per ml ethanol. The effects of the cultivation parameters on these assays were also a part of the study.