Search

Your search keyword '"Akkerdaas, J H"' showing total 2 results
Start Over Author "Akkerdaas, J H" Database OpenAIRE
2 results on '"Akkerdaas, J H"'

1. Plant-based enveloped Ara h 2 bioparticles display exceptional hypo-allergenicity

Author

Castenmiller, C., Stigler, M., Kirpas, M. E., Versteeg, S., Akkerdaas, J. H., Pena-Castellanos, G., Blokhuis, B. R., Dreskin, S. C., Auger, L., Desgagnés, R., Martel, C., Mirande, L., Morel, B., Roberge, J., Stordeur, V., Tropper, G., Vézina, L. P., Gomord, V., de Jong, E. C., Redegeld, F., Shreffler, W. G., Aglas, L., van Ree, R., Afd Pharmacology, and Pharmacology

Subjects
food allergy, hypoallergenicity, plant-based particle, Immunology, allergen immunotherapy, peanut allergy, Immunology and Allergy, bioparticle
Published
2023

2. How far can we simplify in vitro diagnostics for Fagales tree pollen allergy? A study with three whole pollen extracts and purified natural and recombinant allergens

Author

van Ree, R., van Leeuwen, W. A., Akkerdaas, J. H., Aalberse, R. C., and Other departments

Abstract

Current diagnostic tests for Fagales tree pollen allergy are often composed of mixtures of pollen of birch, alder and hazel. Their complex composition hampers accurate standardization. The aim of this study was to investigate whether mixtures of tree pollen extracts can be replaced by a single pollen species, and whether a single pollen species can be replaced by a limited number of purified natural or recombinant major allergens. Sera (n = 1725) were selected on ground of a general suspicion for inhalant allergy, and tested in a RAST for birch, alder and hazel pollen. Sera with > 0.5 RU/mL for any of the three species were tested in a RAST for natural Bet v 1 and Bet v 2 as well as for recombinant versions of both allergens. Specific IgE antibodies (> 0.3 RU/mL) against birch, alder and hazel were found in 242, 298 and 292 sera, respectively. All sera with a positive RAST for alder and/or hazel and a negative RAST for birch were low-responder sera on alder and hazel, only five sera having a RAST value > 1.0 (all < 2.0). For all sera with a RAST > 0.5 RU/mL (n = 250), the mean of individual ratio's alder/birch and hazel/birch was 1.02 and 0.54, respectively. Of 223 of these sera, 63.2% had specific IgE against natural Bet v 1 and 63.7% against natural Bet v 2. When responses to both allergens were combined 93.7% were positive. The mean ratios Bet v 1 + 2/extract were 1.00, 1.04 and 2. 11 in case of birch, alder and hazel, respectively. For 211 sera the same analysis was performed with recombinant Bet v 1 and Bet v 2. Only six sera with Bet v 1-specific IgE (all < 0.5 RU/mL) were negative ( 1.0 RU/mL. The mean of the individual recombinant/natural ratios was 0. 98 for Bet v 1 and 0.38 for Bet v 2 (P < 0.001). The mean ratio rBet v 1 + 2/birch was 0.75 with 17.5% false-negatives on the combination of recombinant allergens. Reliable in vitro diagnosis is possible with a single tree pollen extract (birch or alder). The same is true for purified natural Bet v 1 and Bet v 2. A combination of recombinant molecules is slightly less efficient

Published
1999

Catalog

Books, media, physical & digital resources
See catalog results