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For centuries, traditional medicine from plants (phytotherapy) was the only treatment for infectious and non-infectious diseases. Although it is still practiced in several countries with excellent therapeutic results, it is frequently underestimated because, unlike Western medicine, it is not based on an empirical scientific foundation. However, interest in the search for plant-based therapeutic resources has been stimulated by disciplines such as phytochemistry and the side effects of conventional pharmacological therapies. For example

Aquaculture is an important source of food and livelihood for hundreds of millions of people around the world, however, aquaculture systems are affected by different factors, among them the appearance of resistant or multiresistant bacteria to antimicrobials. The secondary metabolites of plants have been proposed as alternatives for the treatment of these bacteria. The aim of the present study was to determine the antibacterial activity of Caesalpinia coriaria fruit hydroalcoholic extract and gallic acid over Aeromonas hydrophila, Aeromonas veronii, and Aeromonas dhakensis to identify new molecules for the treatment of diseases caused by Aeromonas spp. The C. coriaria fruit hydroalcoholic extract (HECc) was obtained by hydroalcoholic maceration and subjected to bipartition with ethyl acetate and water to obtain an aqueous fraction (Ac-FrCc) and an organic fraction (Ac-FrEtCc); gallic acid was purchased commercially. The Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), MBC/MIC ratio, and cytotoxicity of HECc, its fractions, and gallic acid were determined. The results indicate that HECc fractions (Ac-FrCc and Ac-FrEtCc) and gallic acid have bactericidal activity against A. hydrophila and A. dhakensis, but only gallic acid showed bactericidal activity against A. veronii. The HECc and Ac-FrCc showed no toxicity, Ac-FrEtCc showed low toxicity, and gallic acid showed medium toxicity. The HECc, Ac-FrCc, and Ac-FrEtCc may be alternatives for the treatment of diseases caused by the genus Aeromonas, however, in vivo assays are necessary to corroborate these results.

Canine distemper virus (CDV) is the causative agent of one of the most spread, highly contagious and lethal viral disease in canines, whose morbidity varies between 25-75% and mortality between 50-90%. The aim of the present study was to carry out an epidemiological study of CDV from a Veterinary Hospital in the city of Pachuca de Soto, State of Hidalgo. The information was obtained from 7280 medical records of canine patients treated during the 2017-2018 period, of which 65 met the positive case criteria for the CDV. An observational, cross-sectional epidemiological study with a retrospective search of cases was carried out. It was determined that 8 out of every 1000 patients attending the clinic were positive for CDV, observing that a higher frequency of males with 63%, with Relative Risk (RR) of 0.67 and an Odds Ratio (OR) of 0.47 , patients under 6 months have the highest frequency of cases with 62% (RR of 8.0 and OR of 19.2); Despite the variability of the breeds within the analysis performed, the highest frequency was found in mongrel dogs with 52% (RR of 1.79 and OR of 2.66), on the other hand, it was determined that seasonality influences the degree of presentation of this disease, being greater in winter with 45% of cases (RR 1.81 and OR 2.47). In conclusion, in the Veterinary Hospital under study, the CDV affects male Creole dogs less than seven months of age unvaccinated more and the disease occurs most frequently in winter

Objective: To isolate and identify the exact species of the genus Mycobacterium from Didelphis (D.) virginiana, and the direct implications of this bacterium to public health and veterinary medicine. Methods: Thirty-one D. virginiana were captured and necropsied in Hidalgo, Mexico. Tissue samples were collected to culture mycobacteria present and examine individual specimens’ histopathology. Mycobacterium identification was obtained through the application of amplification and sequencing of 16S rDNA techniques. Results: Three strains were isolated and identified as Mycobacterium (M.) avium subsp. hominissuis by utilizing M. avium complex- specific primers. Granulomatous lesions were observed in the subpleural zone (granuloma grade Π ) and bronchial (granuloma grade I ) of the lungs of D. virginiana with positive isolation. Conclusions: Three strains of M. avium subsp. hominissuis, from lung tissue samples of D. virginiana were identified. This subspecies of M. avium has important implications in public health and veterinary medicine.

Goat production in Mexico is an important economic activity that is affected by different gastrointestinal nematode (GIN) species. GINs resistant to commercial anthelmintics have been reported. Plant extracts or agro-industrial by-products, such as coffee pulp, have been proposed as control alternatives, given their secondary metabolite content. The aim of the present study was to determine the anthelmintic activity of the hydroalcoholic extract of coffee pulp against benzimidazole-resistant GINs. Stool samples were collected from goats, from which GIN eggs were identified and quantified. Molecular techniques confirmed the genus of GINs and their benzimidazole resistance profile. The percentage of egg hatching inhibition (% EHI) and larval mortality (% LM) with the hydroalcoholic extract of coffee pulp was determined at concentrations from 200 to 0.39 mg/mL. The genera Haemonchus spp. and Trichostrongylus spp. were identified, and the presence of the β-tubulin gene mutation, associated with benzimidazole (BZ) resistance, was determined. Hydroalcoholic extract of coffee pulp inhibited 100% of egg hatching at 200 and 100 mg/mL, with no larvicidal effect at the evaluated concentrations.

Se evaluó el comportamiento productivo de ovejas en pastoreo con y sin suplementación en épocas críticas y el comportamiento de los corderos en la Facultad de Agronomía y Veterinaria de la Universidad Autónoma de San Luis Potosí, a 22.23° LN y a 100.85° LO, a 1,835 m.s.n.m. en clima seco templado. Se utilizaron 24 hembras ovinas de la raza Rambouillet, con un peso promedio de 55 kg en praderas irrigadas de festuca alta (Festuca arundinaceae). Se utilizaron tres tratamientos, 1) solo pastoreo, 2) 300 gr/día de suplemento (rastrojo + pollinaza 10% + zeolita 5%) por oveja. y 3) 400 gr/día de suplemento (rastrojo + pollinaza 20% + zeolita 5%) por oveja. A las ovejas se les midió el Peso al empadre (PE), al parto (PP) y al destete (PD), Condición corporal al empadre (CCE), parto (CCP) y destete (CCD); a los corderos se les midió Peso al nacimiento del cordero (PNC) y destete (PDC), además Ganancia de Peso de Hembras (GPH), y Ganancia diaria de peso de corderos (GDPC). Se utilizó un modelo estadístico completamente al azar. Peso, ganancia de peso y condición corporal de hembras se vio favorecida por la suplementación estratégica al suministrar mayores nutrientes en las etapas de empadre, último tercio de gestación y lactancia, lo que se reflejó en mayores pesos de los corderos al nacimiento y destete

Sea urchins are a group of benthic invertebrates characterized by having rigid globose bodies, covered in spines, and have an innate immune system that has allowed them to survive in the environment and defend against many pathogens that affect them. They are consumed for their unique flavor, but also for possessing a rich source of bioactive compounds which make them a source for a wide array of medicinal properties. Thus, these may be used to discover and develop new drugs such as anti-bacterials, anti-carcinogenics and anti-virals. Precisely for those reasons, this revision is centered on the known biological activities in various sea urchin species. Recently, the potential pharmacological benefits of nine sea urchin species [Diadema antillarum (Philippi 1845), Echinometra mathaei (de Blainville), Evechinus chloroticus (Valenciennes), Mesocentrotus nudus (Agassiz, 1863), Paracentrotus lividus (Lamarck, 1816), Scaphechinus mirabilis (Agazzis, 1863), Stomopneustes variolaris (Lamarck, 1816), Tripneustes depressus (Agassiz, 1863), and Tripneustes ventricosus (Lamarck, 1816)] have been evaluated. Our work includes a comprehensive review of the anti-fungal, anti-parasitic, anti-inflammatory, hepatoprotective, anti-viral, anti-diabetic, anti-lipidemic, gastro-protective and anti-cardiotoxic effects. Furthermore, we revised the compounds responsible of these pharmacological effects. This work was intended for a broad readership in the fields of pharmacology, drugs and devices, marine biology and aquaculture, fisheries and fish science. Our results suggest that organic extracts, as well as pure compounds obtained from several parts of sea urchin bodies are effective in vitro and in vivo pharmacological models. As such, these properties manifest the potential use of sea urchins to develop emergent active ingredients.

For centuries, traditional medicine from plants (phytotherapy) was the only treatment for infectious and non-infectious diseases. Although it is still practiced in several countries with excellent therapeutic results, it is frequently underestimated because, unlike Western medicine, it is not based on an empirical scientific foundation. However, interest in the search for plant-based therapeutic resources has been stimulated by disciplines such as phytochemistry and the side effects of conventional pharmacological therapies. For example, Larrea tridentata is a perennial shrub used in traditional medicine in northern Mexico and the southern United States to treat infertility, rheumatism, arthritis, colds, diarrhea, skin problems, pain, inflammation and excess body weight. Scientific research has revealed its beneficial effects—antioxidant, antitumor, neuroprotective, regenerative, antibacterial, antiviral, antifungal, anthelmintic, antiprotozoal and insecticidal—although reports indicate that some compounds in Larrea tridentata may be hepatotoxic and nephrotoxic. Therefore, the aim of this review was to highlight the updates regarding phytochemical compounds and the pharmacological properties of Larrea tridentata.

Simple Summary Pyrethroid insecticides are extensively used in controlling agricultural insects and treatment of ectoparasitic infestation in farm animals. However, the unhygienic disposable and seepage of pyrethroids from the agricultural runoff will lead to contamination of the aquatic ecosystems, which will, in turn, induce harmful toxic effects in the exposed living aquatic organisms, including fish. Cypermethrin (CYP) is a commonly and widely used type II pyrethroid insecticide with known dangerous toxic effects on the exposed organisms. Serious hazardous effects of these toxicants have been reported in several fish species leading to high mortalities and economic losses of the exposed fish. Abstract Pesticides are chemicals used to control pests, such as aquatic weeds, insects, aquatic snails, and plant diseases. They are extensively used in forestry, agriculture, veterinary practices, and of great public health importance. Pesticides can be categorized according to their use into three major types (namely insecticides, herbicides, and fungicides). Water contamination by pesticides is known to induce harmful impacts on the production, reproduction, and survivability of living aquatic organisms, such as algae, aquatic plants, and fish (shellfish and finfish species). The literature and information present in this review article facilitate evaluating the toxic effects from exposure to various fish species to different concentrations of pesticides. Moreover, a brief overview of sources, classification, mechanisms of action, and toxicity signs of pyrethroid insecticides in several fish species will be illustrated with special emphasis on Cypermethrin toxicity.

Due to the emergence of bacterial resistance in phytopathogenic microorganisms, it is necessary to search for new treatment alternatives for these pathogens. Natural extracts are a potential source of bioactive compounds that can act against such bacterial strains. The antibacterial activity of Larrea tridentata against bacteria with public health importance has been documented, however, few reports cover pathogens associated with the agricultural sector. The aim of the present study was to evaluate the antibacterial activity of Larrea tridentata hydroalcoholic extract (LTHE) and fractions against phytopathogenic bacteria. LTHE was obtained by the maceration technique and then subjected to bipartition using solvents of different polarities. Clavibacter michiganensis sbsp. michiganensis, Pseudomonas syringae, and Xanthomonas campestris strains were used, and their antibiotic sensitivity was determined. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of LTHE and its fractions (aqueous: LTAq-F, organic: LTEtOAc-F) were determined. LTHE and its organic fraction showed bactericidal activity against the three bacteria, showing better activity against X. campestris, exhibiting an MIC of 0.39 mg/mL and an MBC of 0.78 mg/mL. The results show that LTHE and its organic fraction have bactericidal activity in vitro against Clavibacter michiganensis sbsp. michiganensis, Pseudomonas syringae, and Xanthomonas campestris.

Coccidiosis in rabbit production is responsible for high morbidity, mortality, and economic losses. The use of natural antimicrobial substances in rabbits represents a promising way to improve their health and production. The aim of the present study was to assess the activity of Salix babylonica hydroalcoholic extract (SBHE) on the elimination of Eimeria spp. in rabbits. The phytochemical compounds and chemical composition of SBHE were determined. The cytotoxicity of SBHE was determined by a microwell assay using Artemia salina. Twenty-five New Zealand rabbits, 28 days old and 872 ± 171 g body weight (BW), were used in a completely randomized design. The rabbits were assigned to five groups of five rabbits, control group (A) received only basal diet (BD), B group received BD + 25 mg/kg BW of SBHE, C group received BD + 50 mg/kg BW of SBHE, D group received BD + 100 mg/kg BW of SBHE, and E group received BD + coccidiostat Baycox® (75 mg/kg body weight) for 28 days. Feces samples were collected at days 0, 7, 14, 21, and 28; morphological and morphometric identifications of Eimeria were carried out by the flotation technique and counting of oocysts by the McMaster technique. The rabbits were found naturally infected with Eimeria spp. The SBHE present phytochemicals with anticoccidial activity, and the cytotoxicity test indicate that this extract is nontoxic. This study demonstrates that oral administration of SBHE at 25 and 50 mg/kg BW reduced the release of oocysts per gram of feces. This effect was observed at day 14 and had the most significant effect at day 28 for both concentrations. The results indicate that SBHE could be a natural alternative for the control of coccidiosis in rabbit production.

Bovine mastitis is one of the most common diseases in dairy cows, and it causes significant economic losses in dairy industries worldwide. Gram-positive and Gram-negative bacteria can cause bovine mastitis, and many of them have developed antimicrobial resistance. There is an urgent need for novel therapeutic options to treat the disease. Larrea tridentata-derived compounds represent an important potential alternative treatment. The aim of the present study was to isolate and characterize antibacterial compounds from Larrea tridentata against multidrug-resistant bacteria associated with bovine mastitis. The L. tridentata hydroalcoholic extract (LTHE) exhibited antibacterial activity. The extract was subjected to a bipartition, giving an aqueous fraction (moderate antibacterial activity) and an organic fraction (higher antibacterial activity). Chromatographic separation of the organic fraction enabled us to obtain four active sub-fractions. Chemical analyses through HPLC techniques were conducted for the LTHE, fractions, and sub-fraction Ltc1-F3, from which we isolated two compounds, characterized by 1H and 13C NMR analyses. Compound nor-3 demethoxyisoguaiacin exhibited the best antibacterial activity against the evaluated bacteria (MIC: 0.01–3.12 mg/mL; MBC: 0.02–3.12 mg/mL). The results indicated that nor-3 demethoxyisoguaiacin can be used as an alternative treatment for multidrug-resistant bacteria associated with mastitis.

The resistance of microbial strain to the use of medically important antibiotics, high cost of production, and the resistance of ecto and endoparasite to anthelminthic and acaricidal is a cause for concern. There has been intensified effort in search for alternatives to synthetic drugs. Such alternative must be able to kill, reduce, or inhibit pathogenic microbial population while improving the commensal microbes’. Black cumin (Nigella sativa Linn.), pawpaw (Carica papaya Linn.) and mustard (Brassica nigra Linn.) seeds fit into those categories. The antimicrobial functions of black seeds, is preventing the formation of biofilm among microbial strain. The glucosinolate compound in it could be degraded in 48 h by incubating it with fungi (Aspergillus sp. NR-4201) strain. Similarly, Enterobacter cloacae is capable of degrading benzyl isothiocyanate content of mustard. The 15% inclusion of mustard oil in vitro was capable of reducing methane formation. Sinapine a derivative of mustard is cable of enhancing the growth of some microbes except Escherichia coli and thus a potential probiotics. Pawpaw seed is very potent in their control of wide range of ecto and endo parasites. However, seeds of black cumin, pawpaw and mustard might be incorporated into livestock nutrition.

The case of a 2.5-year-old sheep sent for diagnostic evaluation is presented. At the necropsy, mucoid degeneration of coronary fat, pleural adhesions, hypostatic congestion, hemoperitoneum, reticulum, rumen, omasum, and abomasum distention were found. The most significant finding was the whitish, hard-to-touch appearance of the right atrium. The atrial sample was decalcified for sectioning and histopathology study. The histology of the section performed revealed: extensive areas of cartilaginous metaplasia, swelling, and undulation of cardiac muscle fibers, fatty infiltration between cardiac fibers, as well as figures compatible with Sarcocystis. The clinical chemistry indicated hyperphosphatemia and hypermagnesemia. According to the process for the sheep diagnostic evaluation, cartilaginous metaplasia of the right atrium, associated with the ingestion of Trisetum flavescens was found. In the different animal species, the probability of progressive poisoning from the consumption of toxic plants is a risk that can occur in any animal production system.

The present report describes a schwannoma type III found in a 12-year-old male SharPei dog, this neoplasm was excised and analyzed using histopathological and immunohistochemistry examinations. This schwannoma was classified as high malignancy and presented metastasis to the axillary node. A secondary bacterial septicemia was identified with routine and molecular techniques. The Inmunohistochemical diagnosis of the mass, corroborated the presumptive diagnosis, which suggested a Schwannoma as well as its malignancy degree. In addition, Leucobacter chromiiresistens was isolated, a specie of which to date there are no reports as a pathogen in animals or humans.

RESUMEN Objetivo Determinar el comportamiento temporal y espacial de la leptospirosis en México durante el periodo 2013-2019. Materiales y Métodos Se utilizó la información depositada en los boletines epidemiológicos del Sistema Nacional de Vigilancia Epidemiológica de México. Para determinar el comportamiento espacial se construyeron canales endémicos y análisis de series de tiempo. Para determinar el comportamiento espacial, se utilizó el modelo estadístico espacial Scan. Resultados Se determinó que los casos de leptospirosis en México se presentan todo el año; sin embargo, los picos endémicos se observaron en los meses de agosto, septiembre y octubre. Con respecto al comportamiento espacial, no se determinó ninguna agrupación estadísticamente; no obstante, los estados con la mayor prevalencia fueron: Sinaloa, con 146.7 casos por cada millón de habitantes, y Tabasco, con 142 casos por cada millón de habitantes. Discusión La leptospirosis es una enfermedad influenciada por los hábitos y las costumbres de la población, al igual que por variables climatológicas que favorecen el contacto con el agente etiológico. Esto coincide con lo reportado por otros estudios que han determinado que la incidencia de leptospirosis aumenta durante la época de lluvia y que esta enfermedad se relaciona con actividades de recreación en zonas tropicales. Conclusiones La leptospirosis es una enfermedad zoonótica emergente de gran importancia en México, que se presenta con mayor frecuencia en época de lluvias. Los estados de Sinaloa y Tabasco son los más afectados. ABSTRACT Objetive To determine the temporal and spatial behavior of leptospirosis in Mexico during the period 2013-2019. Materials and Methods It was used the information deposited in the epidemiological bulletins of National System of Epidemiological Surveillance of Mexico. To determine spatial behavior, endemic channels and time series analyzes were constructed. The spatial statistical model Scan was used to determine the spatial behavior. Results It was determined that leptospirosis cases in Mexico occur throughout the year, however endemic peaks were observed during the months of August, September and October. Regarding to spatial behavior, it was not statistically determined any aggrupation, nevertheless the States with the highest prevalence were Sinaloa with 146.7 cases per million of habitants and Tabasco with 142 cases per million of habitants. Discussion Leptospirosis is a disease which is affected by habits and customs of the populations, as well as by climatological events, favoring the contact with the etiological agent, which coincides with that reported in other studies, in which it was determined that leptospirosis incidence increases during the rainy season, likewise, it was reported that this disease is related to recreational activities in tropical areas. Conclusions To date leptospirosis is an important emerging zoonotic disease in Mexico, the disease occurs more frequently during rainy season, being Sinaloa and Tabasco the most affected States by this disease.

Diabetes mellitus (DM) and coronavirus disease 2019 (COVID-19) are public health issues worldwide, and their comorbidities trigger the progress to severe disease and even death in such patients. Globally, DM has affected an estimated 9.3% adults, and as of April 18, 2021, the World Health Organization (WHO) has confirmed 141,727,940 COVID-19 confirmed cases. The virus is spread via droplets, aerosols, and direct touch with others. Numerous predictive factors have been linked to COVID-19 severity, including impaired immune response and increased inflammatory response, among others. Angiotensin receptor blockers and angiotensin converting enzyme 2 have also been identified as playing a boosting role in both susceptibility and severity to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Specifically, in DM patients, both their control and management during this pandemic is herculean as the restriction periods have markedly hampered the maintenance of means to control glycemia, hypertension, and neuroendocrine and kidney diseases. In addition, as a result of the underlyin cardio-metabolic and immunological disorders, DM patients are at a higher risk of developing the severe form of COVID-19 despite other comorbidities, such as hypertension, also potentially boosting the development of higher COVID-19 severity. However, even in non-DM patients, SARS-CoV-2 may also cause transient hyperglycemia through induction of insulin resistance and/or pancreatic β-cell injury. Therefore, a strict glucose monitoring of DM patients with COVID-19 is mandatory to prevent life-threatening complications.

Overweight and obesity are defined as an unnecessary accumulation of fat, which poses a risk to health. It is a well-identified risk factor for increased mortality due to heightened rates of heart disease, certain cancers, musculoskeletal disorders, and bacterial, protozoan and viral infections. The increasing prevalence of obesity is of concern, as conventional pathogenesis may indeed be increased in obese hosts rather than healthy hosts, especially during this COVID-19 pandemic. COVID-19 is a new disease and we do not have the luxury of cumulative data. Obesity activates the development of gene induced hypoxia and adipogenesis in obese animals. Several factors can influence obesity, for example, stress can increase the body weight by allowing people to consume high amounts of food with a higher propensity to consume palatable food. Obesity is a risk factor for the development of immune-mediated and some inflammatory-mediated diseases, including atherosclerosis and psoriasis, leading to a dampened immune response to infectious agents, leading to weaker post-infection impacts. Moreover, the obese host creates a special microenvironment for disease pathogenesis, marked by persistent low-grade inflammation. Therefore, it is advisable to sustain healthy eating habits by increasing the consumption of various plant-based and low-fat foods to protect our bodies and decrease the risk of infectious diseases, especially COVID-19.

Pepper originated from the Capsicum genus, which is recognized as one of the most predominant and globally distributed genera of the Solanaceae family. It is a diverse genus, consisting of more than 31 different species including five domesticated species, Capsicum baccatum, C. annuum, C. pubescen, C. frutescens, and C. chinense. Pepper is the most widely used spice in the world and is highly valued due to its pungency and unique flavor. Pepper is a good source of provitamin A; vitamins E and C; carotenoids; and phenolic compounds such as capsaicinoids, luteolin, and quercetin. All of these compounds are associated with their antioxidant as well as other biological activities. Interestingly, Capsicum fruits have been used as food additives in the treatment of toothache, parasitic infections, coughs, wound healing, sore throat, and rheumatism. Moreover, it possesses antimicrobial, antiseptic, anticancer, counterirritant, appetite stimulator, antioxidant, and immunomodulator activities. Capsaicin and Capsicum creams are accessible in numerous ways and have been utilized in HIV-linked neuropathy and intractable pain.

Simple Summary Aquaculture development is limited by several diseases associated with bacteria that have developed resistance to antibiotics. In this context, new treatment alternatives are necessary. The aim of the present study was to evaluate the in vitro antibacterial effects of Salix babylonica hydro-alcoholic extract against some bacteria that affect rainbow trout (Oncorhynchus mykiss) and tilapia (Oreochromis spp.). The results indicate that Salix babylonica hydro-alcoholic extract has an antibacterial effect and could be an alternative treatment in diseases caused by microorganisms resistant to drugs in aquaculture. Abstract Aquaculture development is limited by bacteria associated with several diseases; antibiotics are used for the treatment of these affections, but bacteria have developed resistance to these drugs. It is important to develop effective treatments that allow the production of antibiotic-free food. The aim of the present study is to evaluate the in vitro antibacterial effects of Salix babylonica hydro-alcoholic extract (SbHE) against Aeromonas hydrophila, Listonella anguillarum, Edwarsiella tarda, and Streptococcus iniae, bacteria that affect Oncorhynchus mykiss and Oreochromis spp. production. SbHE was obtained through the maceration technique. Reference strains were used and their sensitivity to antibiotics was determined. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of SbHE were determined. Results showed that three of four evaluated bacteria were multidrug resistant, except S. iniae. SbHE showed antibacterial activity against all bacteria. Results indicate an MIC of 1.56 to 25 mg/mL and an MBC of 3.12 to 100 mg/mL. The greatest inhibitory activity occurred against L. anguillarum obtaining a MIC of 1.56 mg/mL and an MBC of 3.12 mg/mL. Results indicate that SbHE has bactericidal activity against A. hydrophila, L. anguilalurm, and S. iniae as well as bacteriostatic activity against E. tarda and could be an alternative treatment against these bacteria.

Plants have been used since ancient times to cure certain infectious diseases, and some of them are now standard treatments for several diseases. Due to the side effects and resistance of pathogenic microorganisms to antibiotics and most drugs on the market, a great deal of attention has been paid to extracts and biologically active compounds isolated from plant species used in herbal medicine. Artemisia absinthium is an important perennial shrubby plant that has been widely used for the treatment of several ailments. Traditionally, A. absinthium has always been of pharmaceutical and botanical importance and used to manage several disorders including hepatocyte enlargement, hepatitis, gastritis, jaundice, wound healing, splenomegaly, dyspepsia, indigestion, flatulence, gastric pain, anemia, and anorexia. It has also been documented to possess antioxidant, antifungal, antimicrobial, anthelmintic, anti-ulcer, anticarcinogenic, hepatoprotective, neuroprotective, antidepressant, analgesic, immunomodulatory, and cytotoxic activity. Long-term use of A. absinthium essential oil may cause toxic and mental disorders in humans with clinical manifestations including convulsions, sleeplessness, and hallucinations. Combination chemotherapies of artemisia extract or its isolated active constituents with the currently available antibabesial or anti-malarial drugs are now documented to relieve malaria and piroplasmosis infections. The current review examines the phytoconstituents, toxic and biological activities of A. absinthium.

Plants have been used since ancient times to cure certain infectious diseases, and some of them are now standard treatments for several diseases. Due to the side effects and resistance of pathogenic microorganisms to antibiotics and most drugs on the market, a great deal of attention has been paid to extracts and biologically active compounds isolated from plant species used in herbal medicine. Artemisia absinthium is an important perennial shrubby plant that has been widely used for the treatment of several ailments. Traditionally, A. absinthium has always been of pharmaceutical and botanical importance and used to manage several disorders including hepatocyte enlargement, hepatitis, gastritis, jaundice, wound healing, splenomegaly, dyspepsia, indigestion, flatulence, gastric pain, anemia, and anorexia. It has also been documented to possess antioxidant, antifungal, antimicrobial, anthelmintic, anti-ulcer, anticarcinogenic, hepatoprotective, neuroprotective, antidepressant, analgesic, immunomodulatory, and cytotoxic activity. Long-term use of A. absinthium essential oil may cause toxic and mental disorders in humans with clinical manifestations including convulsions, sleeplessness, and hallucinations. Combination chemotherapies of artemisia extract or its isolated active constituents with the currently available antibabesial or anti-malarial drugs are now documented to relieve malaria and piroplasmosis infections. The current review examines the phytoconstituents, toxic and biological activities of A. absinthium.

Rattlesnakes have venoms with a complex toxin mixture comprised of polypeptides and proteins. Previous studies have shown that some of these polypeptides are of high value for the development of new medical treatments. The aim of the present study is to evaluate, in vitro, the antibacterial and hemolytic activity of Crotalus triseriatus and Crotalus ravus venoms. A direct field search was conducted to obtain Crotalus triseriatus and Crotalus ravus venom samples. These were evaluated to determine their antibacterial activity against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa through the techniques of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Hemolytic activity was also determined. Antibacterial activity was determined for treatments (Crotalus triseriatus 2) CT2 and (Crotalus ravus 3) CR3, obtaining a Minimum Inhibitory Concentration of 50 &micro, g/mL and a Minimum Bactericidal Concentration of 100 &micro, g/mL against Pseudomonas aeruginosa. CT1 (Crotalus triseriatus 1), CT2, and CR3 presented hemolytic activity, on the other hand, Crotalus ravus 4 (CR4) did not show hemolytic activity. The results of the present study indicate for the first time that Crotalus triseriatus and Crotalus ravus venoms contain some bioactive compounds with bactericidal activity against Pseudomonas aeruginosa which could be used as alternative treatment in diseases caused by this pathogenic bacterium.

Simple Summary Rattlesnakes (Crotalus ravus and Crotalus triseriatus) have some compounds that resemble polypeptides and proteins in their venoms which can be used in therapeutic treatment as antibacterial compounds. The aim of the present study is to evaluate the antibacterial and hemolytic activity of two rattlesnake venoms. The results of the present study indicate that the evaluated venoms have bactericidal activity against Pseudomonas aeruginosa, an important bacterium that affects animals and humans, thereby providing a new and efficient treatment alternative against this pathogenic bacterium. Abstract Rattlesnakes have venoms with a complex toxin mixture comprised of polypeptides and proteins. Previous studies have shown that some of these polypeptides are of high value for the development of new medical treatments. The aim of the present study is to evaluate, in vitro, the antibacterial and hemolytic activity of Crotalus triseriatus and Crotalus ravus venoms. A direct field search was conducted to obtain Crotalus triseriatus and Crotalus ravus venom samples. These were evaluated to determine their antibacterial activity against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa through the techniques of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Hemolytic activity was also determined. Antibacterial activity was determined for treatments (Crotalus triseriatus 2) CT2 and (Crotalus ravus 3) CR3, obtaining a Minimum Inhibitory Concentration of 50 µg/mL and a Minimum Bactericidal Concentration of 100 µg/mL against Pseudomonas aeruginosa. CT1 (Crotalus triseriatus 1), CT2, and CR3 presented hemolytic activity; on the other hand, Crotalus ravus 4 (CR4) did not show hemolytic activity. The results of the present study indicate for the first time that Crotalus triseriatus and Crotalus ravus venoms contain some bioactive compounds with bactericidal activity against Pseudomonas aeruginosa which could be used as alternative treatment in diseases caused by this pathogenic bacterium.

The excessive use of antibiotics, has generated resistance of microorganisms to these, have been searched effectives alternatives for treating diseases caused by resistant or multiresistant microorganism, within of these alternatives are plants, which by its content of secondary compounds have antibacterial activity. The aim on the present experiment was characterize and determine the antibacterial activity of methanolic extract of Salix babylonica (SB) against important bacteria in public health. To obtain extract, the maceration technique was used, qualitative and quantitative (gas chromatography) chemical characterization was carried. For antibacterial activity, the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) was determined, the characterization of the extract allowed the identification of phenolic compounds, coumarins, lactones, flavonols, quinones, saponins, triterpenes and steroidal compounds, also Thymol (0.5319 mg/mL) and Carvacrol (0.4158 mg/mL). The extract showed the best activity against Bacillus. subtilis (MIC: 12.5 mg/mL and WBC: 25 mg/mL), Listeria. monocytogenes and Staphylococcus. aureus (MIC: 25 mg/mL and MBC: 50 mg/mL). It is concluded that the methanolic extract of SB can be an alternative for the treatment of diseases produced by resistant or multiresistant bacteria to antibiotics.

The World Organization for Animal Health (OIE) mentions that the inappropriate use of antibiotics has led to the emergence of strains of bacteria resistant to the mechanisms of action of these drugs, due to the scarcity of effective therapies, the development of new treatment options is required for diseases that affect health, in this respect plant extracts or their pure compounds offer an alternative. The aim of the present investigation was to characterize and evaluate the in vitro antibacterial activity of the hydroalcoholic extract of Croton draco on bacteria of sanitary importance. The extract was obtained by hydroalcoholic maceration, a qualitative and chemical characterization of the extract was carried out, and the antibacterial activity was determined by the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC). The qualitative characterization tests performed on the hydroalcoholic extract of Croton draco indicate the presence of phenolic compounds, terpenes, saponins and alkaloids, by gas chromatography the presence of thymol and carvacrol was determined in concentrations of 0.5340 mg/ml and 0.4206 mg/mL respectively. The bacteria showed different degrees of sensitivity to the hydroalcoholic extract, however, greater activity was determined against Gram positive bacteria such as Listeria monocytogenes, Staphylococcus aureus and Bacillus subtilis.

Mycobacteriumgenus causes a variety of zoonotic diseases. The best known example is the zoonotic tuberculosis due toM. bovis. Much less is known about “nontuberculous mycobacteria (NTM),” which are also associated with infections in humans. The Mexican standard NOM-ZOO-031-1995 regulates the presence ofM. bovisin cattle; however, no regulation exists for the NTM species. The objective of this study was to isolate and identify nontuberculous mycobacteria species from cattle of local herds in the south region of the State of Mexico through the identification and detection of the 100 bp molecular marker in the 23S rRNA gene with subsequent sequencing of the 16S rRNA gene. Milk samples (35) and nasal exudate samples (68) were collected. From the 108 strains isolated, 39 were selected for identification. Thirteen strains isolated from nasal exudates amplified the 100 bp molecular marker and were identified asM. neoaurum(six strains),M. parafortuitum(four strains),M. moriokaense(two strains), andM. confluentis(one strain). ExceptM. parafortuitum, the other species identified are of public health and veterinary concern because they are pathogenic to humans, especially those with underlying medical conditions.

espanolResumen El objetivo del presente trabajo fue determinar el porcentaje de bovinos para abasto positivos a clorhidrato de clembuterol (CCL), con base en muestras de orina de bovinos sacrificados en 3 rastros del estado de Mexico. Para esto se colectaron muestras en los rastros municipales de Toluca (59), Ixtlahuaca (52) y Atlacomulco (40), y se obtuvo un total de 151 muestras. El analisis de las muestras se realizo a traves de la prueba de ELISA y para el reporte de resultados se utilizo estadistica descriptiva. De las muestras analizadas 105 fueron positivas (69,53 %), por rastro la positividad, fue de 48, 30 y 27 respectivamente. Al establecer diferentes rangos de concentracion a CCL, en el rango de 200 a 1999 pg g-1 se ubicaron 46 muestras; 6 muestras, en el rango de 2000 a 3525 pg g-1; 3, en el rango de 3526 a 5050 pg g-1; 1 muestra, entre 5051 y 6575 pg g-1, y 95 se ubicaron entre 6575 y >8100 pg g-1. El rastro con mayor porcentaje de positividad fue el de Toluca, con base en los rangos establecidos, y las concentraciones mas elevadas tambien se observaron en el mismo rastro. El limite minimo de deteccion de CCL de 2000 pg g-1 permite considerar que, con base tanto en la concentracion como en su cinetica, las cantidades detectadas en el estudio sirven como un indicador importante de residualidad a traves de este tipo de muestra. EnglishAbstract The present work aimed to determine the percentage of bovines that tested positive for clenbuterol hydrochloride (CCL), based on urine samples of cattle slaughtered in 3 slaughterhouses of the State of Mexico. To the effect, samples were collected in the municipal slaughterhouses of Toluca (59), Ixtlahuaca (52), and Atlacomulco (40), resulting in a total of 151 samples. Sample analysis was carried out using the ELISA test, and descriptive statistics were used to report the results. 105 of the analyzed samples were positive (69.53%); by slaughterhouse, positivity was 48, 30, and 27, respectively. When establishing different ranges of CCL concentration, 46 samples were in the range of 200 to 1999 pg g-1; 6 samples were in the range of 2000 to 3525 pg g-1; 3 were in the range of 3526 to 5050 pg g-1; 1 sample was between 5051 and 6575 pg g-1, and 95 samples were between 6575 and >8100 pg g-1. The slaughterhouse with the highest percentage of positivity was that of Toluca, based on the established ranges, and the highest concentrations were also observed there. The minimum limit for the detection of CCL (2000 pg g-1) allows considering that, based on both concentration and kinetics, the quantities detected in the study serve as an important indicator of residuality in this type of sample. portuguesResumo O objetivo do presente trabalho foi determinar a porcentagem de bovinos para fornecimento, positivos ao cloridrato de Clenbuterol (CCL), baseados em amostras de urina de bovinos abatidos em tres trilhas do estado de Mexico. Para isso, foram coletadas amostras nas trilhas municipais de Toluca (59), Ixtlahuaca (52) e Atlacomulco (40), e obtivera-se um total de 151 amostras. A analise das amostras realizou-se atraves do teste de ELISA e para o relatorio de resultados foi usada estatistica descritiva. Das amostras analisadas 105 foram positivas (69,53 %), pelo traco a positividade foi de 48, 30 e 27 respetivamente. Ao estabelecer diferentes faixas de concentracao a CCL, 46 amostras foram colocadas na faixa de 200 a 1999 pg g-1; 6 amostras na faixa de 2000 a 3525 pg g-1; 3 na faixa de 3526 a 5050 pg g-1; 1 amostra entre 5051 e 6575 pg g-1, e 95 entre 6575 e >8100 pg g-1. A trilha com maior porcentagem de positividade foi a de Toluca, baseada nas faixas estabelecidas e as concentracoes mais altas observaram-se na mesma trilha. O limite minimo de deteccao de CCL de 2000 pg g-1 permite considerar que, baseado na concentracao e na cinetica, as quantidades detectadas no estudo servem como indicador importante de residualidade atraves deste tipo de amostra.

Due to the resistance that parasites have acquired to conventional drugs, new strategies are currently being sought to control the cycle of parasites associated with health problems in animal production. The objective of the study was to determine the anthelmintic activity of Leucaena leucocephala pod (LLP) hydroalcoholic extract on gastrointestinal nematodes of sheep. A hydroalcoholic extract of LLP was prepared and the concentrations 50, 25, 12.5 and 6.25 mg/mL were evaluated to determine the activity on eggs hatching inhibition of gastrointestinal nematodes in sheep and the mortality on larvae of nematodes (L3). It was determined that the hydroalcoholic extract of LLP at 50 mg / mL inhibited the eggs hatching of nematode (20%) and at 6.25 mg/mL killed 22% of the larvae of nematodes (L3). The hydroalcoholic extract of LLP contains active metabolites that act in inhibition eggs hatching on field nematodes and on infective L3. The hydroalcoholic extract of LLP can be an unconventional alternative for the prevention and control of parasites in small ruminant at field level.

Aquaculture development is limited by bacteria associated with several diseases, antibiotics are used for the treatment of these affections, but bacteria have developed resistance to these drugs. It is important to develop effective treatments that allow the production of antibiotic-free food. The aim of the present study is to evaluate the in vitro antibacterial effects of Salix babylonica hydro-alcoholic extract (SbHE) against Aeromonas hydrophila, Listonella anguillarum, Edwarsiella tarda, and Streptococcus iniae, bacteria that affect Oncorhynchus mykiss and Oreochromis spp. production. SbHE was obtained through the maceration technique. Reference strains were used and their sensitivity to antibiotics was determined. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of SbHE were determined. Results showed that three of four evaluated bacteria were multidrug resistant, except S. iniae. SbHE showed antibacterial activity against all bacteria. Results indicate an MIC of 1.56 to 25 mg/mL and an MBC of 3.12 to 100 mg/mL. The greatest inhibitory activity occurred against L. anguillarum obtaining a MIC of 1.56 mg/mL and an MBC of 3.12 mg/mL. Results indicate that SbHE has bactericidal activity against A. hydrophila, L.anguilalurm, and S. iniae as well as bacteriostatic activity against E. tarda and could be an alternative treatment against these bacteria.

Exosomes are extracellular vesicles (EVs) belonging to the nanovesicles family that function as signaling molecules between cells. After their first description in the late 1960s, interest in their potential as a research target has steadily increased. They are small secreted organelles with a single membrane that are well enriched in lipids, proteins, nucleic acids, and glycoconjugates. Exosomes take part in a larger communication network in which cells communicate between one another by DNA shuttling, proteins, RNA, and membrane-bound factors. The machinery of protein quality control occurs through the process termed “exosome biogenesis”. Furthermore, the pathway involved in intercellular movement of vesicles is vital in various aspects of human health and diseases. Due to their inherent properties, exosomes are currently being developed as potential therapeutic agents in a wide range of diseases including infectious and non-infectious diseases. Exosomes and other EVs sourced from Mesenchymal stem cells (MSCs) have been shown in different studies to possess therapeutic effects in diverse disease models either in vivo or in vitro. Some mechanisms and/or pathways that MSC-derived exosomes use to illustrate their therapeutic effect against some diseases have also been summarized. This review aims to highlight the recent findings and potential therapeutic application of exosomes in different diseases such as autoimmune, cardiovascular, obesity, neural, soft tissues, bone, and cartilage.

Antimicrobial resistance has been increasing in recent years and is most frequently found in pathogenic microorganisms resistant or multiresistant to drugs. The secondary metabolites of plants have been evaluated as alternatives for control and treatment of these microorganisms. The aim of this study was to isolate and identify the secondary metabolites with antibacterial activity from Caesalpinia coriaria (Jacq) Willd fruit. Hydroalcoholic extract (CCHA), was subjected to a bipartition with ethyl acetate giving two fractions an aqueous (Aq-F) and an organic (EtOAc-F). The isolation of bioactive fraction (EtOAc-F) allowed obtain two important compounds, methyl gallate (1) and gallic acid (2). These compounds were identified by high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR). The CCHA, both fractions and the isolated compounds were evaluated in vitro to determine their Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) against Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Listeria monocytogenes and Staphylococcus aureus. Gallic acid (2) showed the lowest MIC on S. typhi, (0.156 mg/mL), L. monocytogenes and S. aureus (1.25 mg/mL), while methyl gallate (1) had the best inhibitory effect against E. coli and P. aeruginosa (1.25 mg/mL). On the other hand, methyl gallate (1) showed the best MBC on P. aeruginosa (2.50 mg/mL), and gallic acid (2) had the lowest MBC on P. aeruginosa and L. monocytogenes. In conclusion, methyl gallate (1) and gallic acid (2) are the compounds responsible for the antibacterial activity of Caesalpinia coriaria fruit.

Currently, the use of medicinal plants has become an alternative for treatment and control of diseases in both veterinary and human medicine. Recent studies have reported that secondary metabolites present in all plants have effects on production and animal health as they have bactericidal or bacteriostatic, anthelmintic (tannins and saponins), anticancer, antioxidant and immunostimulant (phenolic compounds, alkaloid saponins, and terpenes) effects; and for their content of secondary metabolites they have been proposed as an alternative in animal feed because can increase productive and reproductive parameters. The presence or absence of these effects depends on the kind of plant, the content of secondary metabolites, the kind of secondary metabolite and the quantity and the frequency in which they are consumed. The aim of the present review was to carry out a bibliography search of the main secondary metabolites of plants with antimicrobial and over gastrointestinal nematodes activity, reported in Veterinary Medicine Keyword: secondary metabolites, antimicrobial, antihelmintic Veterinary Medicine.

The donkeys (Equus africanus asinus), like the rest of the animal species, are susceptible to infestation by ecto and endoparasites, the investigations with respect to parasitosis that affect this species are very scarce and it is assumed that symptoms, pathogeny, treatment, and control, are similar to those observed in horses. The aim of the present investigation was to identify the main species of gastrointestinal parasites in donkeys of Tulancingo Valley. Eleven donkeys with an average age of two years were used which were managed according to international bioethical standards. Feces were collected and processed to observe the eggs morphology of gastrointestinal parasites and quantification the eggs by flotation and Mc Master techniques respectively. The 100% of donkeys presented eggs of gastrointestinal parasites, 91% (10/11) presented Trichostrongylus spp, 64% (7/11) Strongylus spp, 36% (4/11) Trichonema spp, 27% (3/11) Parascaris equorum, Strongyloide westeri, 9% (1/11) Anaplocephala spp and Oxyuris equi. It was determined that Trichostrongylus spp, is the main gastrointestinal parasite in donkeys of Tulancingo Valley. Keywords: Equus africanus asinus, Oxyurus eqqi, Parascaris equorum, Strongyloides, Thrichonema, Strongylus.

This study was carried out to assess the effect of the chemical substances of Salix babylonica (SB) extract on in vitro rumen fermentation of five mixed rations with different maize silage to concentrate ratios. Fifty-nine compounds were identified in SB extract using the retention time and mass spectral technique. Interactive effects were noted (P < 0.001) for the asymptotic gas production (GP) (b), the rate of production (c), the initial delay before GP begins (L), pH, dry matter digestibility, metabolizable energy (ME), organic matter digestibility (OMD), short chain fatty acids (SCFAs), gas yield at 24 h (GY_{24}), microbial crude protein, and in vitro GP. Both 1.2 and 1.8 mL SB/g DM had the highest (P < 0.05) b and c values. Addition of 1.2 and 1.8 mL SB/g DM linearly improved (P < 0.001) ME, OMD, SCFAs, and GY24. It could be concluded that, based on the highly detected interaction effects between ration types and extract doses for fermentation parameters and GP, the most effective dose of SB varied between incubated total mixed rations. However, the ration of 25% silage and 75% concentrate had the highest nutritive value, especially at doses of 1.2 to 1.8 mL SB/g DM total mixed ration.

Bovine tuberculosis (bTB) is a chronic infectious disease that affects both domestic animals and wildlife. Veterinary epidemiology studies evaluate bTB using geographic information systems (GIS), which can characterise the spatial and temporal distribution of diseases and identify the geographic areas and animal populations at risk of contracting a disease. The present study used space‑time permutation scan statistic to identify the spatial and temporal distribution of bTB from 2005 to 2010 in the State of Mexico with the goal of creating a similarity model using Mahalanobis Distance to identify areas suitable for bTB occurrence. Three significant clusters were identified using space‑time permutation scan statistic and the similarity model identified several areas with suitable environmental and demographic characteristics. The results demonstrate that the occurrence of bTB in the State of Mexico is not randomly distributed.

The principle of animal wellbeing, which states that animals should be free from pain, injury, and disease, is difficult to maintain, because microorganisms are most frequently found to be resistant or multi-resistant to drugs. The secondary metabolites of plants are an alternative for the treatment of these microorganisms. The aim of this work was to determine the antibacterial effect of Salix babylonica L. hydroalcoholic extract (SBHE) against Escherichia coli, Staphylococcus aureus and Listeria monocytogenes, and identify the compounds associated with the activity. The SBHE showed activity against the three strains, and was subjected to a bipartition, obtaining aqueous fraction (ASB) with moderate activity and organic fraction (ACSB) with good activity against the three strains. The chromatographic separation of ACSB, allowed us to obtain ten fractions (F1AC to F10AC), and only three showed activity (F7AC, F8AC and F10AC). In F7AC, five compounds were identified preliminary by GC-MS, in F8AC and F10AC were identified luteolin (1) and luteolin 7-O-glucoside (2) by HPLC, respectively. The best antibacterial activity was obtained with F7AC (Listeria monocytogenes, MIC: 0.78 mg/mL, MBC: 0.78 mg/mL) and F8AC (Staphylococcus aureus, MIC: 0.39 mg/mL, MBC: 0.78 mg/mL). The results indicated that the compounds obtained from SBHE can be used as an alternative treatment against these microorganisms and, by this mechanism, contribute to animal and human health.

La tuberculosis es una enfermedad crónica diagnosticada por baciloscopía. En 1992, Roller y col. identicaron un marcador molecular característico de bacterias Gram positivas con alto contenido de guanina y citosina. El objetivó de este artículo fue amplicar este marcador molecular en muestras de esputo para detectar la presencia de micobacterias. Se recolectaron 28 muestras de esputo, se les amplicó el marcador molecular, se inocularon en los medios de cultivo Lowenstein-Jensen y Stonebrink y a las cepas aisladas se les amplicó el gen rRNA 16S para su identicación. Seis muestras amplicaron el marcador molecular. Cinco cepas tu vieron una semejanza del 100% con el complejo tuberculosis y una cepa 99% con Mycobacterium conceptionense . Con la amplicación del marcador molecular, se logró detectar la presencia de micobacterias en muestras de esputo.

A region-wide survey was conducted in the tropical area of Tierra Caliente, State of Guerrero, Mexico to estimate the prevalence of subclinical bovine mastitis (SCM), distribution of mastitis pathogens, and in vitro antimicrobial susceptibility of different mastitis pathogens in dairy farms. In total, 1036 quarter milk samples were obtained from 259 cows at 87 different dairy farms. Collected quarter milk samples were submitted for California Mastitis Test (CMT), bacteriological examination, and testing for antimicrobial susceptibility. Overall prevalence of SCM in the studied area was 20.5 %. Prevalence in the different regions was as follows: 28 % in Arcelia municipality, 21 % in Tlalchapa municipality, 19.4 % in Pungarabato municipality, and 14.3 % in Finch Cutzamala municipality. Of all positive isolates, 97.5 % were Gram-negative bacteria. Moreover, of all positive isolates, 37.5 % were Proteus vulgaris, 25 % Salmonella spp., 12.5 % Enterobacter aerogenes, and 10 % Escherichia coli. Klebsiella pneumonia and E. coli were sensitive for netilmicin antimicrobial. However, E. coli was sensitive for pefloxacin and gentamicin with a sensitivity for pefloxacin for E. aerogenes, while Staphylococci were sensitive for gentamicin and dicloxacillin. It could be concluded that practices such as the implementation of mastitis control programs, improved milking hygiene together with an intramammary treatment with netilmicin, pefloxacin, and gentamicin antimicrobials should be considered for mastitis prevention in the study area of Tierra Caliente, in the tropical area of Guerrero, Mexico.

"La tuberculosis es una enfermedad crónica diagnosticada por baciloscopía. En 1992, Roller y col. identi?caron un marcador molecular característico de bacterias Gram positivas con alto contenido de guanina y citosina. El objetivó de este artículo fue ampli?car este marcador molecular en muestras de esputo para detectar la presencia de micobacterias. Se recolectaron 28 muestras de esputo, se les ampli?có el marcador molecular, se inocularon en los medios de cultivo Lowenstein-Jensen y Stonebrink y a las cepas aisladas se les ampli?có el gen rRNA 16S para su identi?cación. Seis muestras ampli?caron el marcador molecular. Cinco cepas tu vieron una semejanza del 100% con el complejo tuberculosis y una cepa 99% con Mycobacterium conceptionense . Con la ampli?cación del marcador molecular, se logró detectar la presencia de micobacterias en muestras de esputo."

Tuberculosis (TB) is one of the oldest human diseases that still affects large population groups. According to the World Health Organization (WHO), there were approximately 9.4 million new cases worldwide in the year 2010. In Mexico, there were 18,848 new cases of TB of all clinical variants in 2010. The identification of clusters in space-time is of great interest in epidemiological studies. The objective of this research was to identify the spatial and temporal distribution of TB during the period 2006–2010 in the State of Mexico, using geographic information system (GIS) and SCAN statistics program. Nine significant clusters () were identified using spatial and space-time analysis. The conclusion is that TB in the State of Mexico is not randomly distributed but is concentrated in areas close to Mexico City.

RESUMEN El objetivo del estudio fue determinar la actividad antibacteriana in vitro del extracto hidroalcohólico de Dalea bicolor sobre bacterias de importancia en salud pública. La actividad antibacteriana se determinó mediante la Concentración Mínima Inhibitoria (CMI) y la Concentración Mínima Bactericida (CMB). Se determinó que el extracto hidroalcohólico de Dalea bicolor posee actividad inhibitoria a concentraciones de 3.12 a 12.5 mg/ml y actividad bactericida a 50 y 100 mg/ml, pudiendo ser considerado como una alternativa para el tratamiento de infecciones asociadas a bacterias de importancia en salud pública. ABSTRACT The aim of this study was to determine the in vitro antibacterial activity of the hydroalcoholic extract of Dalea bicolor on bacteria of importance in public health. The antibacterial activity was determined by the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC). It was determined that the hydroalcoholic extract of Dalea bicolor has inhibitory activity at concentrations of 3.12 to 12.5 mg/ml and bactericidal activity at 50 and 100 mg/ml and can be considered as an alternative for the treatment of infections associated with bacteria of importance in public health.