1. High dye removal capacity of Peniophora laxitexta immobilized in a combined support based on polyurethane foam and lignocellulosic substrates
- Author
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Laura Noemí Levin, Leonardo Matias Majul, and Sonia Alejandra Wirth
- Subjects
Textile ,PENIOPHORA LAXITEXTA ,0208 environmental biotechnology ,Biotecnología del Medio Ambiente ,MIXED SUPPORT ,02 engineering and technology ,INGENIERÍAS Y TECNOLOGÍAS ,010501 environmental sciences ,01 natural sciences ,Peniophora laxitexta ,Biotecnología Industrial ,Ciencias Biológicas ,chemistry.chemical_compound ,LACCASE ,Environmental Chemistry ,Waste Management and Disposal ,Effluent ,IMMOBILIZED FUNGI ,Bioremediación, Diagnóstico Biotecnológico en Gestión Medioambiental ,0105 earth and related environmental sciences ,Water Science and Technology ,Polyurethane ,Laccase ,business.industry ,Chemistry ,DYE REMOVAL ,General Medicine ,Pulp and paper industry ,Bioprocesamiento Tecnológico, Biocatálisis, Fermentación ,020801 environmental engineering ,Wastewater ,Micología ,business ,CIENCIAS NATURALES Y EXACTAS - Abstract
Dye removal from effluents is a major problem for most textile industries. At present, wastewater treatments are currently based on physico-chemical methods which are expensive, energy inefficient and of limited versatility. The aim of this work was to find a low-cost and efficient method for dye removal. To do this, we designed a combined system based on the immobilization of the ligninolytic white-rot fungus Peniophora laxitexta (BAFC 4687) on mixed supports consisting of two polyurethane foam discs and a middle layer of diverse lignocellulosic substrates: soybean hulls, wheat straw or cellulose spent casings. As a corticioid fungus, P. laxitexta was able to completely colonize the supports developing a compact and tight structure that maintained the integrity of the system after several dye removal cycles. The immobilized fungus removed between 30% and 50% of the azoic dye Xylidine and more than 50% of the anthraquinonic dye RBBR in three successive cycles, and near 90% of the triphenylmethane Malachite Green in 10 repeated cycles without any loss of efficiency. Our analysis showed that the removal of the dyes was due to the combined effect of adsorption to the supports and enzymatic decolorization by soluble laccases and enzymes associated with the mycelium. Additionally, we showed that the presence of Malachite Green induced the expression of a new laccase isoform with high decolorization capacity. Based on these results, we propose that this inoculated laminar biocarrier could be effectively used for dye removal in textile wastewater. Fil: Majul, Leonardo Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina Fil: Wirth, Sonia Alejandra. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Levin, Laura Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
- Published
- 2020