1. Drug resistance associated properties of blasts subpopulations with different CD34 expression in childhood acute lymphoblastic leukemia (ALL)
- Author
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T V, Shman, U U, Fedasenka, V P, Savitski, A M, Kustanovich, and O V, Aleinikova
- Subjects
Male ,ATP Binding Cassette Transporter, Subfamily B ,Adolescent ,Infant ,Antigens, CD34 ,Precursor Cell Lymphoblastic Leukemia-Lymphoma ,Neoplasm Proteins ,Proto-Oncogene Proteins c-bcl-2 ,Drug Resistance, Neoplasm ,Cell Line, Tumor ,Child, Preschool ,ATP Binding Cassette Transporter, Subfamily G, Member 2 ,Humans ,ATP-Binding Cassette Transporters ,Female ,ATP Binding Cassette Transporter, Subfamily B, Member 1 ,RNA, Messenger ,Child ,Vault Ribonucleoprotein Particles - Abstract
The objective of this study was to investigate expression of drug resistance associated genes in CD34+ and CD34- leukemic subpopulations in childhood acute lymphoblastic leukemia (ALL).ALL samples with heterogeneous CD34 expression were separated into CD34-positive and CD34-negative subpopulations and mRNA levels of MDR1, LRP, BCRP and BCL-2 genes were compared.BCL-2 gene expression levels did not differ significantly between CD34+ vs CD34- subpopulations in most analyzed ALL cases. Oppositely, MDR1 gene hadtwo-fold differences in expression levels between subpopulations in the majority of ALL cases. In T-lineage ALL CD34- fractions had increased level of BCRP and LRP genes in comparison with CD34+ ones whereas in most of B-lineage ALL expression of these genes did not differ.It was not found the unique pattern of resistance related genes expression in CD34+ vs CD34- subpopulations. However, in majority of studied pediatric ALL cases with CD34 heterogeneous expression one of subpopulations (positive or negative) could have an advantage for survival through elevated expression of drug resistance related genes.
- Published
- 2011