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1. Sulfated β-(1→4)-galacto-oligosaccharides and their effect on angiogenesis

Author

D. H. Paper, Christian W Käsbauer, and Gerhard Franz

Subjects
Molecular Sequence Data, Ion chromatography, Neovascularization, Physiologic, Oligosaccharides, Angiogenesis Inhibitors, Chick Embryo, Polysaccharide, Fibroblast growth factor, Biochemistry, Analytical Chemistry, chemistry.chemical_compound, Sulfation, Allantois, Polysaccharides, Carbohydrate Conformation, Animals, Sulfate, chemistry.chemical_classification, Plants, Medicinal, Chromatography, Organic Chemistry, Fabaceae, Chorion, General Medicine, Oligosaccharide, Angiogenesis inhibitor, Carbohydrate Sequence, chemistry, Carbohydrate conformation
Abstract

Sulfated beta-(1-->4)-galacto-oligosaccharides were prepared from an arabino-galacto-rhamno-galacturonan from Lupinus polyphyllus Lindl. by successive partial hydrolysis and SO3-pyridine sulfation in DMF. The resulting oligosaccharide polysulfates were analyzed by analytical GPC and the sulfate content was determined by ion chromatography. DP 5 and higher showed a pronounced antiangiogenic effect with scores of 0.9-1.2 for DP 7-9 using the CAM-assay. An interaction with the fibroblast growth factor FGF-2 was noticed for DP 4-12 depending on the degree of sulfation using the FGF-2-trypsin assay.

Published
2001

2. LaPSvS1, a (1--3)-beta-galactan sulfate and its effect on angiogenesis in vivo and in vitro

Author

H. Vogl, Robert J. Linhardt, Gerhard Franz, Jutta Bürgermeister, and D. H. Paper

Subjects
Time Factors, Angiogenesis, Anti-Inflammatory Agents, Neovascularization, Physiologic, Angiogenesis Inhibitors, Chick Embryo, Sulfuric Acid Esters, Fibroblast growth factor, Biochemistry, Galactans, Analytical Chemistry, chemistry.chemical_compound, Sulfation, In vivo, Allantois, Animals, Sodium dodecyl sulfate, Plants, Medicinal, Dose-Response Relationship, Drug, Chemistry, Heparin, Sulfates, Organic Chemistry, Sodium Dodecyl Sulfate, Fabaceae, General Medicine, Chorion, Galactan, Arabinose, In vitro, Angiogenesis inhibitor, Spectrometry, Fluorescence, Fibroblast Growth Factor 2
Abstract

LaPSvS1, a highly sulfated branched (1-->3)-beta-galactan was prepared from the arabino-galactan from Larix decidua Miller by partial hydrolysis and subsequent sulfation with SO(3)-pyridine in DMF. The molecular weight was analyzed by GPC and the sulfate content was determined by ion chromatography. LaPSvS1 exhibited good antiangiogenic and antiinflammatory effects in two different modifications of the known CAM-assay. In vitro results obtained in the FGF-2-trypsin-assay and in fluorospectrometric experiments revealed that LaPSvS1 interacts with the fibroblast growth factor 2 system. This interaction is correlated with the in vivo effect of LaPSvS1 on FGF-2 induced angiogenesis.

Published
2002