Search

The SEVA platform (https://seva-plasmids.com) was launched one decade ago, both as a database (DB) and as a physical repository of plasmid vectors for genetic analysis and engineering of Gram-negative bacteria with a structure and nomenclature that follows a strict, fixed architecture of functional DNA segments. While the current update keeps the basic features of earlier versions, the platform has been upgraded not only with many more ready-to-use plasmids but also with features that expand the range of target species, harmonize DNA assembly methods and enable new applications. In particular, SEVA 4.0 includes (i) a sub-collection of plasmids for easing the composition of multiple DNA segments with MoClo/Golden Gate technology, (ii) vectors for Gram-positive bacteria and yeast and [iii] off-the-shelf constructs with built-in functionalities. A growing collection of plasmids that capture part of the standard-but not its entirety-has been compiled also into the DB and repository as a separate corpus (SEVAsib) because of its value as a resource for constructing and deploying phenotypes of interest. Maintenance and curation of the DB were accompanied by dedicated diffusion and communication channels that make the SEVA platform a popular resource for genetic analyses, genome editing and bioengineering of a large number of microorganisms.

The SEVA platform (https://seva-plasmids.com) was launched one decade ago, both as a database (DB) and as a physical repository of plasmid vectors for genetic analysis and engineering of Gram-negative bacteria with a structure and nomenclature that follows a strict, fixed architecture of functional DNA segments. While the current update keeps the basic features of earlier versions, the platform has been upgraded not only with many more ready-to-use plasmids but also with features that expand the range of target species, harmonize DNA assembly methods and enable new applications. In particular, SEVA 4.0 includes (i) a sub-collection of plasmids for easing the composition of multiple DNA segments with MoClo/Golden Gate technology, (ii) vectors for Gram-positive bacteria and yeast and [iii] off-the-shelf constructs with built-in functionalities. A growing collection of plasmids that capture part of the standard-but not its entirety-has been compiled also into the DB and repository as a separate corpus (SEVAsib) because of its value as a resource for constructing and deploying phenotypes of interest. Maintenance and curation of the DB were accompanied by dedicated diffusion and communication channels that make the SEVA platform a popular resource for genetic analyses, genome editing and bioengineering of a large number of microorganisms.

Tree kinds of anti-scratch coatings were applied to protect a polymer water base applied on glass surface. Oleic acid (AP5), silane by CCVD and lacquer by curing UV were evaluated. Each one of them was put in contact with two types of cardboard, laminated and polylaminated using Rub Tester Sutherland 2000TM equipment. At the end of the test, was evaluated the damage level for each sample. The coatings were evaluated both before and after applied the abrasion. Brightness, hardness, superficial tension, transmittance and lubricity were measure in each case searching the performance level of scratch resistance and protection of the prior film. Results show that the best behavior is reached with oleic acid used like lubricant. Also, the aesthetic properties of the first coating are not affected., Tres tipos de recubrimientos fueron evaluados como medio de protección al tallado de un polímero base agua aplicado sobre una superficie vítrica. Las opciones fueron ácido oleico aplicado en fase vapor (AP5), Silano utilizando la técnica de CCVD y un Barniz epóxico de secado UV. Una vez aplicados, cada uno de estos materiales sobre el barniz base agua, fueron puestos en contacto con dos tipos de cartones, poli laminado y laminado a través del método de prueba Rub Tester con el equipo Sutherland 2000TM evaluando el nivel de desgaste para cada caso. Cada uno de los recubrimientos fue caracterizado antes y después de la abrasión, determinando propiedades de brillo, dureza, tensión superficial, transmitancia y lubricidad con el objetivo de conocer el nivel de efectividad de la protección al tallado de la primera capa. Los resultados muestran la mejor protección al tallado aplicando ácido oleico como segunda capa además de tener la ventaja de no afectar las propiedades estéticas del recubrimiento polimérico previo.

Posterior chamber phakic intraocular lens (pIOL) implantation is a common option for correcting moderate-to-high ocular refractive defects. Because this pIOL is implanted on ciliary sulcus, the distance between the back surface of the pIOL and the anterior surface of the crystalline lens, that it is known as vault, should be measured in diferent conditions to ensure the technique’s safety. Cyclopentolate is a drug that dilates the pupil and relaxes accommodation (cycloplegia). It is often used for diferent ocular examinations and for other medical purposes. However, there is no evidence of the efect of this drug on vault. This study quantifed central vault changes associated with cyclopentolate instillation. We measured the vault under normal conditions (pre-cycloplegic instillation) and after instilling cyclopentolate on 39 eyes of 39 patients with implanted pIOL. Our results suggest that cyclopentolate instillation may induce changes to vault in eyes with implanted pIOL. These changes seem safe and are mainly associated with vault under normal conditions, but also with anterior chamber depth, pupillary diameter and pIOL size.

Posterior chamber phakic intraocular lens (pIOL) implantation is a common option for correcting moderate-to-high ocular refractive defects. Because this pIOL is implanted on ciliary sulcus, the distance between the back surface of the pIOL and the anterior surface of the crystalline lens, that it is known as vault, should be measured in diferent conditions to ensure the technique’s safety. Cyclopentolate is a drug that dilates the pupil and relaxes accommodation (cycloplegia). It is often used for diferent ocular examinations and for other medical purposes. However, there is no evidence of the efect of this drug on vault. This study quantifed central vault changes associated with cyclopentolate instillation. We measured the vault under normal conditions (pre-cycloplegic instillation) and after instilling cyclopentolate on 39 eyes of 39 patients with implanted pIOL. Our results suggest that cyclopentolate instillation may induce changes to vault in eyes with implanted pIOL. These changes seem safe and are mainly associated with vault under normal conditions, but also with anterior chamber depth, pupillary diameter and pIOL size.

Posterior chamber phakic intraocular lens (pIOL) implantation is a common option for correcting moderate-to-high ocular refractive defects. Because this pIOL is implanted on ciliary sulcus, the distance between the back surface of the pIOL and the anterior surface of the crystalline lens, that it is known as vault, should be measured in diferent conditions to ensure the technique’s safety. Cyclopentolate is a drug that dilates the pupil and relaxes accommodation (cycloplegia). It is often used for diferent ocular examinations and for other medical purposes. However, there is no evidence of the efect of this drug on vault. This study quantifed central vault changes associated with cyclopentolate instillation. We measured the vault under normal conditions (pre-cycloplegic instillation) and after instilling cyclopentolate on 39 eyes of 39 patients with implanted pIOL. Our results suggest that cyclopentolate instillation may induce changes to vault in eyes with implanted pIOL. These changes seem safe and are mainly associated with vault under normal conditions, but also with anterior chamber depth, pupillary diameter and pIOL size.

Se presenta un conjunto de actividades encaminadas a crear un juego para el aprendizaje del léxico español para estudiantes extranjeros, similar al "Pasapalabras" pero basado en definiciones redactadas con primitivos y moléculas, y en trabajo experimental, realizado todo ello por los estudiantes de Grado de Lengua Española y sus Literaturas, durante el curso 2017/18.

Se presenta un conjunto de actividades encaminadas a crear un juego para el aprendizaje del léxico español para estudiantes extranjeros, similar al "Pasapalabras" pero basado en definiciones redactadas con primitivos y moléculas, y en trabajo experimental, realizado todo ello por los estudiantes de Grado de Lengua Española y sus Literaturas, durante el curso 2017/18.

Documento de trabajo que recoge trece informes realizados por estudiantes del Grado de Lengua Española y sus Literaturas durante el curso 2017/18, que recoge los datos, su análisis y discusión de diversos trabajos experimentales en los que se intenta una aproximación al significado léxico de algunas palabras mediante primitivos y moléculas. Este trabajo se inserta en el Proyecto de Innovación "Piensapalabras" (2017/18, nº 125).