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36 results on '"Sinaniotis, A."'

1. A European-Japanese study on peach allergy: IgE to Pru p 7 associates with severity

Author

MS Dermatologie/Allergologie, Infection & Immunity, MS Reumatologie/Immunologie/Infectie, Regenerative Medicine and Stem Cells, JC onderzoeksprogramma Methodologie, Lab Reumatologie/Klinische Immunologie, Kallen, E. J.J., Revers, A., Fernández-Rivas, M., Asero, R., Ballmer-Weber, B., Barreales, L., Belohlavkova, S., de Blay, F., Clausen, M., Dubakiene, R., Ebisawa, M., Fernández-Perez, C., Fritsche, P., Fukutomi, Y., Gislason, D., Hoffmann-Sommergruber, K., Jedrzejczak-Czechowicz, M., Knulst, A. C., Kowalski, M. L., Kralimarkova, T., Lidholm, J., Metzler, C., Mills, E. N.C., Papadopoulos, N. G., Popov, T. A., Purohit, A., Reig, I., Seneviratne, S. L., Sinaniotis, A., Takei, M., Versteeg, S. A., Vassilopoulou, A. E., Vieths, S., Welsing, P. M.J., Zwinderman, A. H., Le, T. M., Van Ree, R., MS Dermatologie/Allergologie, Infection & Immunity, MS Reumatologie/Immunologie/Infectie, Regenerative Medicine and Stem Cells, JC onderzoeksprogramma Methodologie, Lab Reumatologie/Klinische Immunologie, Kallen, E. J.J., Revers, A., Fernández-Rivas, M., Asero, R., Ballmer-Weber, B., Barreales, L., Belohlavkova, S., de Blay, F., Clausen, M., Dubakiene, R., Ebisawa, M., Fernández-Perez, C., Fritsche, P., Fukutomi, Y., Gislason, D., Hoffmann-Sommergruber, K., Jedrzejczak-Czechowicz, M., Knulst, A. C., Kowalski, M. L., Kralimarkova, T., Lidholm, J., Metzler, C., Mills, E. N.C., Papadopoulos, N. G., Popov, T. A., Purohit, A., Reig, I., Seneviratne, S. L., Sinaniotis, A., Takei, M., Versteeg, S. A., Vassilopoulou, A. E., Vieths, S., Welsing, P. M.J., Zwinderman, A. H., Le, T. M., and Van Ree, R.

Published
2023

2. Development and validation of the Food Allergy Severity Score

Author

Fernández-Rivas, Montserrat, Gómez García, Ismael, Gonzalo-Fernández, Alejandro, Fuentes Ferrer, Manuel, Dölle-Bierke, Sabine, Marco-Martín, Guadalupe, Ballmer-Weber, Barbara K, Asero, Riccardo, Belohlavkova, Simona, Beyer, Kirsten, de Blay, Frédéric, Clausen, Michael, Datema, Mareen R, Dubakiene, Ruta, Grimshaw, Kate E C, Hoffmann-Sommergruber, Karin, Hourihane, Jonathan O'B, Jedrzejczak-Czechowicz, Monika, Knulst, André C, Kralimarkova, Tanya, Le, Thuy-My, Papadopoulos, Nikolaos G, Popov, Todor A, Poulsen, Lars K, Purohit, Ashok, Seneviratne, Suranjith L, Simpson, Angela, Sinaniotis, Atanasios, Turkalji, Mirjana, Vázquez-Cortés, Sonia, Vera-Berrios, Rosialzira N, Muraro, Antonella, Worm, Margitta, Roberts, Graham, van Ree, Ronald, Fernández-Pérez, Cristina, Turner, Paul J, Mills, Elizabeth N Clare, Fernández-Rivas, Montserrat, Gómez García, Ismael, Gonzalo-Fernández, Alejandro, Fuentes Ferrer, Manuel, Dölle-Bierke, Sabine, Marco-Martín, Guadalupe, Ballmer-Weber, Barbara K, Asero, Riccardo, Belohlavkova, Simona, Beyer, Kirsten, de Blay, Frédéric, Clausen, Michael, Datema, Mareen R, Dubakiene, Ruta, Grimshaw, Kate E C, Hoffmann-Sommergruber, Karin, Hourihane, Jonathan O'B, Jedrzejczak-Czechowicz, Monika, Knulst, André C, Kralimarkova, Tanya, Le, Thuy-My, Papadopoulos, Nikolaos G, Popov, Todor A, Poulsen, Lars K, Purohit, Ashok, Seneviratne, Suranjith L, Simpson, Angela, Sinaniotis, Atanasios, Turkalji, Mirjana, Vázquez-Cortés, Sonia, Vera-Berrios, Rosialzira N, Muraro, Antonella, Worm, Margitta, Roberts, Graham, van Ree, Ronald, Fernández-Pérez, Cristina, Turner, Paul J, and Mills, Elizabeth N Clare

Abstract

BACKGROUND: The heterogeneity and lack of validation of existing severity scores for food allergic reactions limit standardization of case management and research advances. We aimed to develop and validate a severity score for food allergic reactions.METHODS: Following a multidisciplinary experts consensus, it was decided to develop a food allergy severity score (FASS) with ordinal (oFASS) and numerical (nFASS) formats. oFASS with 3 and 5 grades were generated through expert consensus, and nFASS by mathematical modeling. Evaluation was performed in the EuroPrevall outpatient clinic cohort (8232 food reactions) by logistic regression with request of emergency care and medications used as outcomes. Discrimination, classification, and calibration were calculated. Bootstrapping internal validation was followed by external validation (logistic regression) in 5 cohorts (3622 food reactions). Correlation of nFASS with the severity classification done by expert allergy clinicians by Best-Worst Scaling of 32 food reactions was calculated.RESULTS: oFASS and nFASS map consistently, with nFASS having greater granularity. With the outcomes emergency care, adrenaline and critical medical treatment, oFASS and nFASS had a good discrimination (receiver operating characteristic area under the curve [ROC-AUC]>0.80), classification (sensitivity 0.87-0.92, specificity 0.73-0.78), and calibration. Bootstrapping over ROC-AUC showed negligible biases (1.0 × 10-6 -1.23 × 10-3 ). In external validation, nFASS performed best with higher ROC-AUC. nFASS was strongly correlated (R 0.89) to best-worst scoring of 334 expert clinicians.CONCLUSION: FASS is a validated and reliable method to measure severity of food allergic reactions. The ordinal and numerical versions that map onto each other are suitable for use by different stakeholders in different settings.

Published
2022

3. Development and validation of the Food Allergy Severity Score

Author

Fernández-Rivas, Montserrat, Gómez García, Ismael, Gonzalo-Fernández, Alejandro, Fuentes Ferrer, Manuel, Dölle-Bierke, Sabine, Marco-Martín, Guadalupe, Ballmer-Weber, Barbara K, Asero, Riccardo, Belohlavkova, Simona, Beyer, Kirsten, de Blay, Frédéric, Clausen, Michael, Datema, Mareen R, Dubakiene, Ruta, Grimshaw, Kate E C, Hoffmann-Sommergruber, Karin, Hourihane, Jonathan O'B, Jedrzejczak-Czechowicz, Monika, Knulst, André C, Kralimarkova, Tanya, Le, Thuy-My, Papadopoulos, Nikolaos G, Popov, Todor A, Poulsen, Lars K, Purohit, Ashok, Seneviratne, Suranjith L, Simpson, Angela, Sinaniotis, Atanasios, Turkalji, Mirjana, Vázquez-Cortés, Sonia, Vera-Berrios, Rosialzira N, Muraro, Antonella, Worm, Margitta, Roberts, Graham, van Ree, Ronald, Fernández-Pérez, Cristina, Turner, Paul J, Mills, Elizabeth N Clare, Fernández-Rivas, Montserrat, Gómez García, Ismael, Gonzalo-Fernández, Alejandro, Fuentes Ferrer, Manuel, Dölle-Bierke, Sabine, Marco-Martín, Guadalupe, Ballmer-Weber, Barbara K, Asero, Riccardo, Belohlavkova, Simona, Beyer, Kirsten, de Blay, Frédéric, Clausen, Michael, Datema, Mareen R, Dubakiene, Ruta, Grimshaw, Kate E C, Hoffmann-Sommergruber, Karin, Hourihane, Jonathan O'B, Jedrzejczak-Czechowicz, Monika, Knulst, André C, Kralimarkova, Tanya, Le, Thuy-My, Papadopoulos, Nikolaos G, Popov, Todor A, Poulsen, Lars K, Purohit, Ashok, Seneviratne, Suranjith L, Simpson, Angela, Sinaniotis, Atanasios, Turkalji, Mirjana, Vázquez-Cortés, Sonia, Vera-Berrios, Rosialzira N, Muraro, Antonella, Worm, Margitta, Roberts, Graham, van Ree, Ronald, Fernández-Pérez, Cristina, Turner, Paul J, and Mills, Elizabeth N Clare

Abstract

BACKGROUND: The heterogeneity and lack of validation of existing severity scores for food allergic reactions limit standardization of case management and research advances. We aimed to develop and validate a severity score for food allergic reactions.METHODS: Following a multidisciplinary experts consensus, it was decided to develop a food allergy severity score (FASS) with ordinal (oFASS) and numerical (nFASS) formats. oFASS with 3 and 5 grades were generated through expert consensus, and nFASS by mathematical modeling. Evaluation was performed in the EuroPrevall outpatient clinic cohort (8232 food reactions) by logistic regression with request of emergency care and medications used as outcomes. Discrimination, classification, and calibration were calculated. Bootstrapping internal validation was followed by external validation (logistic regression) in 5 cohorts (3622 food reactions). Correlation of nFASS with the severity classification done by expert allergy clinicians by Best-Worst Scaling of 32 food reactions was calculated.RESULTS: oFASS and nFASS map consistently, with nFASS having greater granularity. With the outcomes emergency care, adrenaline and critical medical treatment, oFASS and nFASS had a good discrimination (receiver operating characteristic area under the curve [ROC-AUC]>0.80), classification (sensitivity 0.87-0.92, specificity 0.73-0.78), and calibration. Bootstrapping over ROC-AUC showed negligible biases (1.0 × 10-6 -1.23 × 10-3 ). In external validation, nFASS performed best with higher ROC-AUC. nFASS was strongly correlated (R 0.89) to best-worst scoring of 334 expert clinicians.CONCLUSION: FASS is a validated and reliable method to measure severity of food allergic reactions. The ordinal and numerical versions that map onto each other are suitable for use by different stakeholders in different settings.

Published
2022

4. Development and validation of the Food Allergy Severity Score

Author

MS Dermatologie/Allergologie, Infection & Immunity, Fernández-Rivas, Montserrat, Gómez García, Ismael, Gonzalo-Fernández, Alejandro, Fuentes Ferrer, Manuel, Dölle-Bierke, Sabine, Marco-Martín, Guadalupe, Ballmer-Weber, Barbara K., Asero, Riccardo, Belohlavkova, Simona, Beyer, Kirsten, de Blay, Frédéric, Clausen, Michael, Datema, Mareen R., Dubakiene, Ruta, Grimshaw, Kate E.C., Hoffmann-Sommergruber, Karin, Hourihane, Jonathan O.B., Jedrzejczak-Czechowicz, Monika, Knulst, André C., Kralimarkova, Tanya, Le, Thuy My, Papadopoulos, Nikolaos G., Popov, Todor A., Poulsen, Lars K., Purohit, Ashok, Seneviratne, Suranjith L., Simpson, Angela, Sinaniotis, Atanasios, Turkalji, Mirjana, Vázquez-Cortés, Sonia, Vera-Berrios, Rosialzira N., Muraro, Antonella, Worm, Margitta, Roberts, Graham, van Ree, Ronald, Fernández-Pérez, Cristina, Turner, Paul J., Mills, Elizabeth N.Clare, MS Dermatologie/Allergologie, Infection & Immunity, Fernández-Rivas, Montserrat, Gómez García, Ismael, Gonzalo-Fernández, Alejandro, Fuentes Ferrer, Manuel, Dölle-Bierke, Sabine, Marco-Martín, Guadalupe, Ballmer-Weber, Barbara K., Asero, Riccardo, Belohlavkova, Simona, Beyer, Kirsten, de Blay, Frédéric, Clausen, Michael, Datema, Mareen R., Dubakiene, Ruta, Grimshaw, Kate E.C., Hoffmann-Sommergruber, Karin, Hourihane, Jonathan O.B., Jedrzejczak-Czechowicz, Monika, Knulst, André C., Kralimarkova, Tanya, Le, Thuy My, Papadopoulos, Nikolaos G., Popov, Todor A., Poulsen, Lars K., Purohit, Ashok, Seneviratne, Suranjith L., Simpson, Angela, Sinaniotis, Atanasios, Turkalji, Mirjana, Vázquez-Cortés, Sonia, Vera-Berrios, Rosialzira N., Muraro, Antonella, Worm, Margitta, Roberts, Graham, van Ree, Ronald, Fernández-Pérez, Cristina, Turner, Paul J., and Mills, Elizabeth N.Clare

Published
2022

5. Walnut Allergy across Europe: Distribution of Allergen Sensitization Patterns and Prediction of Severity

Author

MS Dermatologie/Allergologie, Infection & Immunity, DIGD-Onderzoek, Lyons, S A, Datema, M R, Le, T T M, Asero, R, Barreales, L, Belohlavkova, S, de Blay, F, Clausen, M, Dubakiene, R, Fernández-Perez, C, Fritsche, P, Gislason, D, Hoffmann-Sommergruber, K, Jedrzejczak-Czechowicz, M, Jongejan, L, Kowalski, M L, Kralimarkova, T, Lidholm, J, Papadopoulos, N G, Pontoppidan, B, Popov, T A, Del Prado, N, Purohit, A, Reig, I, Seneviratne, S L, Sinaniotis, A, Vassilopoulou, E, Versteeg, S A, Vieths, S, Zwinderman, A H, Welsing, P M J, Mills, E N C, Ballmer-Weber, Barbara, Knulst, A C, Fernández-Rivas, Montserrat, Van Ree, Ronald, MS Dermatologie/Allergologie, Infection & Immunity, DIGD-Onderzoek, Lyons, S A, Datema, M R, Le, T T M, Asero, R, Barreales, L, Belohlavkova, S, de Blay, F, Clausen, M, Dubakiene, R, Fernández-Perez, C, Fritsche, P, Gislason, D, Hoffmann-Sommergruber, K, Jedrzejczak-Czechowicz, M, Jongejan, L, Kowalski, M L, Kralimarkova, T, Lidholm, J, Papadopoulos, N G, Pontoppidan, B, Popov, T A, Del Prado, N, Purohit, A, Reig, I, Seneviratne, S L, Sinaniotis, A, Vassilopoulou, E, Versteeg, S A, Vieths, S, Zwinderman, A H, Welsing, P M J, Mills, E N C, Ballmer-Weber, Barbara, Knulst, A C, Fernández-Rivas, Montserrat, and Van Ree, Ronald

Published
2021

6. Component-resolved diagnosis and beyond : Multivariable regression models to predict severity of hazelnut allergy

Author

Datema, M. R., van Ree, R., Asero, R., Barreales, L., Belohlavkova, S., de Blay, F., Clausen, M., Dubakiene, R., Fernández-Perez, C., Fritsche, P., Gislason, D., Hoffmann-Sommergruber, K., Jedrzejczak-Czechowicz, M., Jongejan, L., Knulst, A. C., Kowalski, M., Kralimarkova, T. Z., Le, T. M., Lidholm, J., Papadopoulos, N. G., Popov, T. A., del Prado, N., Purohit, A., Reig, I., Seneviratne, S. L., Sinaniotis, A., Versteeg, S. A., Vieths, S., Zwinderman, A. H., Mills, E. N.C., Fernández-Rivas, M., Ballmer-Weber, B., Datema, M. R., van Ree, R., Asero, R., Barreales, L., Belohlavkova, S., de Blay, F., Clausen, M., Dubakiene, R., Fernández-Perez, C., Fritsche, P., Gislason, D., Hoffmann-Sommergruber, K., Jedrzejczak-Czechowicz, M., Jongejan, L., Knulst, A. C., Kowalski, M., Kralimarkova, T. Z., Le, T. M., Lidholm, J., Papadopoulos, N. G., Popov, T. A., del Prado, N., Purohit, A., Reig, I., Seneviratne, S. L., Sinaniotis, A., Versteeg, S. A., Vieths, S., Zwinderman, A. H., Mills, E. N.C., Fernández-Rivas, M., and Ballmer-Weber, B.

Published
2018

7. Component-resolved diagnosis and beyond : Multivariable regression models to predict severity of hazelnut allergy

Author

Datema, M. R., van Ree, R., Asero, R., Barreales, L., Belohlavkova, S., de Blay, F., Clausen, M., Dubakiene, R., Fernández-Perez, C., Fritsche, P., Gislason, D., Hoffmann-Sommergruber, K., Jedrzejczak-Czechowicz, M., Jongejan, L., Knulst, A. C., Kowalski, M., Kralimarkova, T. Z., Le, T. M., Lidholm, J., Papadopoulos, N. G., Popov, T. A., del Prado, N., Purohit, A., Reig, I., Seneviratne, S. L., Sinaniotis, A., Versteeg, S. A., Vieths, S., Zwinderman, A. H., Mills, E. N.C., Fernández-Rivas, M., Ballmer-Weber, B., Datema, M. R., van Ree, R., Asero, R., Barreales, L., Belohlavkova, S., de Blay, F., Clausen, M., Dubakiene, R., Fernández-Perez, C., Fritsche, P., Gislason, D., Hoffmann-Sommergruber, K., Jedrzejczak-Czechowicz, M., Jongejan, L., Knulst, A. C., Kowalski, M., Kralimarkova, T. Z., Le, T. M., Lidholm, J., Papadopoulos, N. G., Popov, T. A., del Prado, N., Purohit, A., Reig, I., Seneviratne, S. L., Sinaniotis, A., Versteeg, S. A., Vieths, S., Zwinderman, A. H., Mills, E. N.C., Fernández-Rivas, M., and Ballmer-Weber, B.

Published
2018

8. Component-resolved diagnosis and beyond : Multivariable regression models to predict severity of hazelnut allergy

Author

Datema, M. R., van Ree, R., Asero, R., Barreales, L., Belohlavkova, S., de Blay, F., Clausen, M., Dubakiene, R., Fernández-Perez, C., Fritsche, P., Gislason, D., Hoffmann-Sommergruber, K., Jedrzejczak-Czechowicz, M., Jongejan, L., Knulst, A. C., Kowalski, M., Kralimarkova, T. Z., Le, T. M., Lidholm, J., Papadopoulos, N. G., Popov, T. A., del Prado, N., Purohit, A., Reig, I., Seneviratne, S. L., Sinaniotis, A., Versteeg, S. A., Vieths, S., Zwinderman, A. H., Mills, E. N.C., Fernández-Rivas, M., Ballmer-Weber, B., Datema, M. R., van Ree, R., Asero, R., Barreales, L., Belohlavkova, S., de Blay, F., Clausen, M., Dubakiene, R., Fernández-Perez, C., Fritsche, P., Gislason, D., Hoffmann-Sommergruber, K., Jedrzejczak-Czechowicz, M., Jongejan, L., Knulst, A. C., Kowalski, M., Kralimarkova, T. Z., Le, T. M., Lidholm, J., Papadopoulos, N. G., Popov, T. A., del Prado, N., Purohit, A., Reig, I., Seneviratne, S. L., Sinaniotis, A., Versteeg, S. A., Vieths, S., Zwinderman, A. H., Mills, E. N.C., Fernández-Rivas, M., and Ballmer-Weber, B.

Published
2018

9. Component-resolved diagnosis and beyond: Multivariable regression models to predict severity of hazelnut allergy

Author

Arts-assistenten Kinderen, MS Dermatologie/Allergologie, Infection & Immunity, Datema, M. R., van Ree, R., Asero, R., Barreales, L., Belohlavkova, S., de Blay, F., Clausen, M., Dubakiene, R., Fernández-Perez, C., Fritsche, P., Gislason, D., Hoffmann-Sommergruber, K., Jedrzejczak-Czechowicz, M., Jongejan, L., Knulst, A. C., Kowalski, M., Kralimarkova, T. Z., Le, T. M., Lidholm, J., Papadopoulos, N. G., Popov, T. A., del Prado, N., Purohit, A., Reig, I., Seneviratne, S. L., Sinaniotis, A., Versteeg, S. A., Vieths, S., Zwinderman, A. H., Mills, E. N.C., Fernández-Rivas, M., Ballmer-Weber, B., Arts-assistenten Kinderen, MS Dermatologie/Allergologie, Infection & Immunity, Datema, M. R., van Ree, R., Asero, R., Barreales, L., Belohlavkova, S., de Blay, F., Clausen, M., Dubakiene, R., Fernández-Perez, C., Fritsche, P., Gislason, D., Hoffmann-Sommergruber, K., Jedrzejczak-Czechowicz, M., Jongejan, L., Knulst, A. C., Kowalski, M., Kralimarkova, T. Z., Le, T. M., Lidholm, J., Papadopoulos, N. G., Popov, T. A., del Prado, N., Purohit, A., Reig, I., Seneviratne, S. L., Sinaniotis, A., Versteeg, S. A., Vieths, S., Zwinderman, A. H., Mills, E. N.C., Fernández-Rivas, M., and Ballmer-Weber, B.

Published
2018

10. Hazelnut allergy across Europe dissected molecularly : A EuroPrevall outpatient clinic survey

Author

Datema, Mareen R., Zuidmeer-Jongejan, Laurian, Asero, Riccardo, Barreales, Laura, Belohlavkova, Simona, de Blay, Frederic, Bures, Peter, Clausen, Michael, Dubakiene, Ruta, Gislason, David, Jedrzejczak-Czechowicz, Monika, Kowalski, Marek L., Knulst, Andre C., Kralimarkova, Tanya, Le, Thuy-My, Lovegrove, Alison, Marsh, Justin, Papadopoulos, Nikolaos G., Popov, Todor, del Prado, Nayade, Purohit, Ashok, Reese, Gerald, Reig, Isabel, Seneviratne, Suranjith L., Sinaniotis, Athanasios, Versteeg, Serge A., Vieths, Stefan, Zwinderman, Aeilko H., Mills, Clare, Lidholm, Jonas, Hoffmann-Sommergruber, Karin, Fernandez-Rivas, Montserrat, Ballmer-Weber, Barbara, van Ree, Ronald, Datema, Mareen R., Zuidmeer-Jongejan, Laurian, Asero, Riccardo, Barreales, Laura, Belohlavkova, Simona, de Blay, Frederic, Bures, Peter, Clausen, Michael, Dubakiene, Ruta, Gislason, David, Jedrzejczak-Czechowicz, Monika, Kowalski, Marek L., Knulst, Andre C., Kralimarkova, Tanya, Le, Thuy-My, Lovegrove, Alison, Marsh, Justin, Papadopoulos, Nikolaos G., Popov, Todor, del Prado, Nayade, Purohit, Ashok, Reese, Gerald, Reig, Isabel, Seneviratne, Suranjith L., Sinaniotis, Athanasios, Versteeg, Serge A., Vieths, Stefan, Zwinderman, Aeilko H., Mills, Clare, Lidholm, Jonas, Hoffmann-Sommergruber, Karin, Fernandez-Rivas, Montserrat, Ballmer-Weber, Barbara, and van Ree, Ronald

Published
2015

11. Erratum : IgE recognition patterns in peanut allergy are age dependent: Perspectives of the EuroPrevall study (Allergy (2015) 70 (391-406))

Author

Ballmer-Weber, B. K., Lidholm, J., Fernández-Rivas, M., Seneviratne, S., Hanschmann, K. M., Vogel, L., Bures, P., Fritsche, P., Summers, C., Knulst, A. C., Le, T. M., Reig, I., Papadopoulos, N. G., Sinaniotis, A., Belohlavkova, S., Popov, T., Kralimarkova, T., De Blay, F., Purohit, A., Clausen, M., Kowalski, M. L., Asero, R., Dubakiene, R., Barreales, L., Clare Mills, E. N., Van Ree, R., Vieths, S., Ballmer-Weber, B. K., Lidholm, J., Fernández-Rivas, M., Seneviratne, S., Hanschmann, K. M., Vogel, L., Bures, P., Fritsche, P., Summers, C., Knulst, A. C., Le, T. M., Reig, I., Papadopoulos, N. G., Sinaniotis, A., Belohlavkova, S., Popov, T., Kralimarkova, T., De Blay, F., Purohit, A., Clausen, M., Kowalski, M. L., Asero, R., Dubakiene, R., Barreales, L., Clare Mills, E. N., Van Ree, R., and Vieths, S.

Published
2015

12. The EuroPrevall outpatient clinic study on food allergy : Background and methodology

Author

Fernández-Rivas, Montserrat, Barreales, L., Mackie, A. R., Fritsche, P., Vázquez-Cortés, S., Jedrzejczak-Czechowicz, M., Kowalski, M. L., Clausen, M., Gislason, D., Sinaniotis, A., Kompoti, E., Le, T. M., Knulst, A. C., Purohit, A., De Blay, F., Kralimarkova, T., Popov, T., Asero, R., Belohlavkova, S., Seneviratne, S. L., Dubakiene, R., Lidholm, J., Hoffmann-Sommergruber, K., Burney, P., Crevel, R., Brill, M., Fernández-Pérez, C., Vieths, S., Clare Mills, E. N., Van Ree, R., Ballmer-Weber, B. K., Fernández-Rivas, Montserrat, Barreales, L., Mackie, A. R., Fritsche, P., Vázquez-Cortés, S., Jedrzejczak-Czechowicz, M., Kowalski, M. L., Clausen, M., Gislason, D., Sinaniotis, A., Kompoti, E., Le, T. M., Knulst, A. C., Purohit, A., De Blay, F., Kralimarkova, T., Popov, T., Asero, R., Belohlavkova, S., Seneviratne, S. L., Dubakiene, R., Lidholm, J., Hoffmann-Sommergruber, K., Burney, P., Crevel, R., Brill, M., Fernández-Pérez, C., Vieths, S., Clare Mills, E. N., Van Ree, R., and Ballmer-Weber, B. K.

Published
2015

13. Erratum : IgE recognition patterns in peanut allergy are age dependent: Perspectives of the EuroPrevall study (Allergy (2015) 70 (391-406))

Author

Ballmer-Weber, B. K., Lidholm, J., Fernández-Rivas, M., Seneviratne, S., Hanschmann, K. M., Vogel, L., Bures, P., Fritsche, P., Summers, C., Knulst, A. C., Le, T. M., Reig, I., Papadopoulos, N. G., Sinaniotis, A., Belohlavkova, S., Popov, T., Kralimarkova, T., De Blay, F., Purohit, A., Clausen, M., Kowalski, M. L., Asero, R., Dubakiene, R., Barreales, L., Clare Mills, E. N., Van Ree, R., Vieths, S., Ballmer-Weber, B. K., Lidholm, J., Fernández-Rivas, M., Seneviratne, S., Hanschmann, K. M., Vogel, L., Bures, P., Fritsche, P., Summers, C., Knulst, A. C., Le, T. M., Reig, I., Papadopoulos, N. G., Sinaniotis, A., Belohlavkova, S., Popov, T., Kralimarkova, T., De Blay, F., Purohit, A., Clausen, M., Kowalski, M. L., Asero, R., Dubakiene, R., Barreales, L., Clare Mills, E. N., Van Ree, R., and Vieths, S.

Published
2015

14. The EuroPrevall outpatient clinic study on food allergy : Background and methodology

Author

Fernández-Rivas, Montserrat, Barreales, L., Mackie, A. R., Fritsche, P., Vázquez-Cortés, S., Jedrzejczak-Czechowicz, M., Kowalski, M. L., Clausen, M., Gislason, D., Sinaniotis, A., Kompoti, E., Le, T. M., Knulst, A. C., Purohit, A., De Blay, F., Kralimarkova, T., Popov, T., Asero, R., Belohlavkova, S., Seneviratne, S. L., Dubakiene, R., Lidholm, J., Hoffmann-Sommergruber, K., Burney, P., Crevel, R., Brill, M., Fernández-Pérez, C., Vieths, S., Clare Mills, E. N., Van Ree, R., Ballmer-Weber, B. K., Fernández-Rivas, Montserrat, Barreales, L., Mackie, A. R., Fritsche, P., Vázquez-Cortés, S., Jedrzejczak-Czechowicz, M., Kowalski, M. L., Clausen, M., Gislason, D., Sinaniotis, A., Kompoti, E., Le, T. M., Knulst, A. C., Purohit, A., De Blay, F., Kralimarkova, T., Popov, T., Asero, R., Belohlavkova, S., Seneviratne, S. L., Dubakiene, R., Lidholm, J., Hoffmann-Sommergruber, K., Burney, P., Crevel, R., Brill, M., Fernández-Pérez, C., Vieths, S., Clare Mills, E. N., Van Ree, R., and Ballmer-Weber, B. K.

Published
2015

15. Erratum : IgE recognition patterns in peanut allergy are age dependent: Perspectives of the EuroPrevall study (Allergy (2015) 70 (391-406))

Author

Ballmer-Weber, B. K., Lidholm, J., Fernández-Rivas, M., Seneviratne, S., Hanschmann, K. M., Vogel, L., Bures, P., Fritsche, P., Summers, C., Knulst, A. C., Le, T. M., Reig, I., Papadopoulos, N. G., Sinaniotis, A., Belohlavkova, S., Popov, T., Kralimarkova, T., De Blay, F., Purohit, A., Clausen, M., Kowalski, M. L., Asero, R., Dubakiene, R., Barreales, L., Clare Mills, E. N., Van Ree, R., Vieths, S., Ballmer-Weber, B. K., Lidholm, J., Fernández-Rivas, M., Seneviratne, S., Hanschmann, K. M., Vogel, L., Bures, P., Fritsche, P., Summers, C., Knulst, A. C., Le, T. M., Reig, I., Papadopoulos, N. G., Sinaniotis, A., Belohlavkova, S., Popov, T., Kralimarkova, T., De Blay, F., Purohit, A., Clausen, M., Kowalski, M. L., Asero, R., Dubakiene, R., Barreales, L., Clare Mills, E. N., Van Ree, R., and Vieths, S.

Published
2015

16. Hazelnut allergy across Europe dissected molecularly : A EuroPrevall outpatient clinic survey

Author

Datema, Mareen R., Zuidmeer-Jongejan, Laurian, Asero, Riccardo, Barreales, Laura, Belohlavkova, Simona, de Blay, Frederic, Bures, Peter, Clausen, Michael, Dubakiene, Ruta, Gislason, David, Jedrzejczak-Czechowicz, Monika, Kowalski, Marek L., Knulst, Andre C., Kralimarkova, Tanya, Le, Thuy-My, Lovegrove, Alison, Marsh, Justin, Papadopoulos, Nikolaos G., Popov, Todor, del Prado, Nayade, Purohit, Ashok, Reese, Gerald, Reig, Isabel, Seneviratne, Suranjith L., Sinaniotis, Athanasios, Versteeg, Serge A., Vieths, Stefan, Zwinderman, Aeilko H., Mills, Clare, Lidholm, Jonas, Hoffmann-Sommergruber, Karin, Fernandez-Rivas, Montserrat, Ballmer-Weber, Barbara, van Ree, Ronald, Datema, Mareen R., Zuidmeer-Jongejan, Laurian, Asero, Riccardo, Barreales, Laura, Belohlavkova, Simona, de Blay, Frederic, Bures, Peter, Clausen, Michael, Dubakiene, Ruta, Gislason, David, Jedrzejczak-Czechowicz, Monika, Kowalski, Marek L., Knulst, Andre C., Kralimarkova, Tanya, Le, Thuy-My, Lovegrove, Alison, Marsh, Justin, Papadopoulos, Nikolaos G., Popov, Todor, del Prado, Nayade, Purohit, Ashok, Reese, Gerald, Reig, Isabel, Seneviratne, Suranjith L., Sinaniotis, Athanasios, Versteeg, Serge A., Vieths, Stefan, Zwinderman, Aeilko H., Mills, Clare, Lidholm, Jonas, Hoffmann-Sommergruber, Karin, Fernandez-Rivas, Montserrat, Ballmer-Weber, Barbara, and van Ree, Ronald

Published
2015

17. Hazelnut allergy across Europe dissected molecularly : A EuroPrevall outpatient clinic survey

Author

Datema, Mareen R., Zuidmeer-Jongejan, Laurian, Asero, Riccardo, Barreales, Laura, Belohlavkova, Simona, de Blay, Frederic, Bures, Peter, Clausen, Michael, Dubakiene, Ruta, Gislason, David, Jedrzejczak-Czechowicz, Monika, Kowalski, Marek L., Knulst, Andre C., Kralimarkova, Tanya, Le, Thuy-My, Lovegrove, Alison, Marsh, Justin, Papadopoulos, Nikolaos G., Popov, Todor, del Prado, Nayade, Purohit, Ashok, Reese, Gerald, Reig, Isabel, Seneviratne, Suranjith L., Sinaniotis, Athanasios, Versteeg, Serge A., Vieths, Stefan, Zwinderman, Aeilko H., Mills, Clare, Lidholm, Jonas, Hoffmann-Sommergruber, Karin, Fernandez-Rivas, Montserrat, Ballmer-Weber, Barbara, van Ree, Ronald, Datema, Mareen R., Zuidmeer-Jongejan, Laurian, Asero, Riccardo, Barreales, Laura, Belohlavkova, Simona, de Blay, Frederic, Bures, Peter, Clausen, Michael, Dubakiene, Ruta, Gislason, David, Jedrzejczak-Czechowicz, Monika, Kowalski, Marek L., Knulst, Andre C., Kralimarkova, Tanya, Le, Thuy-My, Lovegrove, Alison, Marsh, Justin, Papadopoulos, Nikolaos G., Popov, Todor, del Prado, Nayade, Purohit, Ashok, Reese, Gerald, Reig, Isabel, Seneviratne, Suranjith L., Sinaniotis, Athanasios, Versteeg, Serge A., Vieths, Stefan, Zwinderman, Aeilko H., Mills, Clare, Lidholm, Jonas, Hoffmann-Sommergruber, Karin, Fernandez-Rivas, Montserrat, Ballmer-Weber, Barbara, and van Ree, Ronald

Published
2015

18. The EuroPrevall outpatient clinic study on food allergy : Background and methodology

Author

Fernández-Rivas, Montserrat, Barreales, L., Mackie, A. R., Fritsche, P., Vázquez-Cortés, S., Jedrzejczak-Czechowicz, M., Kowalski, M. L., Clausen, M., Gislason, D., Sinaniotis, A., Kompoti, E., Le, T. M., Knulst, A. C., Purohit, A., De Blay, F., Kralimarkova, T., Popov, T., Asero, R., Belohlavkova, S., Seneviratne, S. L., Dubakiene, R., Lidholm, J., Hoffmann-Sommergruber, K., Burney, P., Crevel, R., Brill, M., Fernández-Pérez, C., Vieths, S., Clare Mills, E. N., Van Ree, R., Ballmer-Weber, B. K., Fernández-Rivas, Montserrat, Barreales, L., Mackie, A. R., Fritsche, P., Vázquez-Cortés, S., Jedrzejczak-Czechowicz, M., Kowalski, M. L., Clausen, M., Gislason, D., Sinaniotis, A., Kompoti, E., Le, T. M., Knulst, A. C., Purohit, A., De Blay, F., Kralimarkova, T., Popov, T., Asero, R., Belohlavkova, S., Seneviratne, S. L., Dubakiene, R., Lidholm, J., Hoffmann-Sommergruber, K., Burney, P., Crevel, R., Brill, M., Fernández-Pérez, C., Vieths, S., Clare Mills, E. N., Van Ree, R., and Ballmer-Weber, B. K.

Published
2015

19. Erratum : IgE recognition patterns in peanut allergy are age dependent: Perspectives of the EuroPrevall study (Allergy (2015) 70 (391-406))

Author

Ballmer-Weber, B. K., Lidholm, J., Fernández-Rivas, M., Seneviratne, S., Hanschmann, K. M., Vogel, L., Bures, P., Fritsche, P., Summers, C., Knulst, A. C., Le, T. M., Reig, I., Papadopoulos, N. G., Sinaniotis, A., Belohlavkova, S., Popov, T., Kralimarkova, T., De Blay, F., Purohit, A., Clausen, M., Kowalski, M. L., Asero, R., Dubakiene, R., Barreales, L., Clare Mills, E. N., Van Ree, R., Vieths, S., Ballmer-Weber, B. K., Lidholm, J., Fernández-Rivas, M., Seneviratne, S., Hanschmann, K. M., Vogel, L., Bures, P., Fritsche, P., Summers, C., Knulst, A. C., Le, T. M., Reig, I., Papadopoulos, N. G., Sinaniotis, A., Belohlavkova, S., Popov, T., Kralimarkova, T., De Blay, F., Purohit, A., Clausen, M., Kowalski, M. L., Asero, R., Dubakiene, R., Barreales, L., Clare Mills, E. N., Van Ree, R., and Vieths, S.

Published
2015

20. Erratum: IgE recognition patterns in peanut allergy are age dependent: Perspectives of the EuroPrevall study (Allergy (2015) 70 (391-406))

Author

MS Dermatologie/Allergologie, Infection & Immunity, Arts-assistenten Kinderen, Ballmer-Weber, B. K., Lidholm, J., Fernández-Rivas, M., Seneviratne, S., Hanschmann, K. M., Vogel, L., Bures, P., Fritsche, P., Summers, C., Knulst, A. C., Le, T. M., Reig, I., Papadopoulos, N. G., Sinaniotis, A., Belohlavkova, S., Popov, T., Kralimarkova, T., De Blay, F., Purohit, A., Clausen, M., Kowalski, M. L., Asero, R., Dubakiene, R., Barreales, L., Clare Mills, E. N., Van Ree, R., Vieths, S., MS Dermatologie/Allergologie, Infection & Immunity, Arts-assistenten Kinderen, Ballmer-Weber, B. K., Lidholm, J., Fernández-Rivas, M., Seneviratne, S., Hanschmann, K. M., Vogel, L., Bures, P., Fritsche, P., Summers, C., Knulst, A. C., Le, T. M., Reig, I., Papadopoulos, N. G., Sinaniotis, A., Belohlavkova, S., Popov, T., Kralimarkova, T., De Blay, F., Purohit, A., Clausen, M., Kowalski, M. L., Asero, R., Dubakiene, R., Barreales, L., Clare Mills, E. N., Van Ree, R., and Vieths, S.

Published
2015

21. The EuroPrevall outpatient clinic study on food allergy: Background and methodology

Author

Infection & Immunity, MS Dermatologie/Allergologie, Arts-assistenten Kinderen, Fernández-Rivas, Montserrat, Barreales, L., Mackie, A. R., Fritsche, P., Vázquez-Cortés, S., Jedrzejczak-Czechowicz, M., Kowalski, M. L., Clausen, M., Gislason, D., Sinaniotis, A., Kompoti, E., Le, T. M., Knulst, A. C., Purohit, A., De Blay, F., Kralimarkova, T., Popov, T., Asero, R., Belohlavkova, S., Seneviratne, S. L., Dubakiene, R., Lidholm, J., Hoffmann-Sommergruber, K., Burney, P., Crevel, R., Brill, M., Fernández-Pérez, C., Vieths, S., Clare Mills, E. N., Van Ree, R., Ballmer-Weber, B. K., Infection & Immunity, MS Dermatologie/Allergologie, Arts-assistenten Kinderen, Fernández-Rivas, Montserrat, Barreales, L., Mackie, A. R., Fritsche, P., Vázquez-Cortés, S., Jedrzejczak-Czechowicz, M., Kowalski, M. L., Clausen, M., Gislason, D., Sinaniotis, A., Kompoti, E., Le, T. M., Knulst, A. C., Purohit, A., De Blay, F., Kralimarkova, T., Popov, T., Asero, R., Belohlavkova, S., Seneviratne, S. L., Dubakiene, R., Lidholm, J., Hoffmann-Sommergruber, K., Burney, P., Crevel, R., Brill, M., Fernández-Pérez, C., Vieths, S., Clare Mills, E. N., Van Ree, R., and Ballmer-Weber, B. K.

Published
2015

22. Hazelnut allergy across Europe dissected molecularly: A EuroPrevall outpatient clinic survey

Author

Infection & Immunity, MS Dermatologie/Allergologie, Datema, Mareen R., Zuidmeer-Jongejan, Laurian, Asero, Riccardo, Barreales, Laura, Belohlavkova, Simona, de Blay, Frederic, Bures, Peter, Clausen, Michael, Dubakiene, Ruta, Gislason, David, Jedrzejczak-Czechowicz, Monika, Kowalski, Marek L., Knulst, Andre C., Kralimarkova, Tanya, Le, Thuy-My, Lovegrove, Alison, Marsh, Justin, Papadopoulos, Nikolaos G., Popov, Todor, del Prado, Nayade, Purohit, Ashok, Reese, Gerald, Reig, Isabel, Seneviratne, Suranjith L., Sinaniotis, Athanasios, Versteeg, Serge A., Vieths, Stefan, Zwinderman, Aeilko H., Mills, Clare, Lidholm, Jonas, Hoffmann-Sommergruber, Karin, Fernandez-Rivas, Montserrat, Ballmer-Weber, Barbara, van Ree, Ronald, Infection & Immunity, MS Dermatologie/Allergologie, Datema, Mareen R., Zuidmeer-Jongejan, Laurian, Asero, Riccardo, Barreales, Laura, Belohlavkova, Simona, de Blay, Frederic, Bures, Peter, Clausen, Michael, Dubakiene, Ruta, Gislason, David, Jedrzejczak-Czechowicz, Monika, Kowalski, Marek L., Knulst, Andre C., Kralimarkova, Tanya, Le, Thuy-My, Lovegrove, Alison, Marsh, Justin, Papadopoulos, Nikolaos G., Popov, Todor, del Prado, Nayade, Purohit, Ashok, Reese, Gerald, Reig, Isabel, Seneviratne, Suranjith L., Sinaniotis, Athanasios, Versteeg, Serge A., Vieths, Stefan, Zwinderman, Aeilko H., Mills, Clare, Lidholm, Jonas, Hoffmann-Sommergruber, Karin, Fernandez-Rivas, Montserrat, Ballmer-Weber, Barbara, and van Ree, Ronald

Published
2015

23. Further studies on the biological activity of hazelnut allergens

Author

Blanc, F, Bernard, H, Ah-Leung, S, Przybylski-Nicaise, L, Skov, P Stahl, Purohit, A, de Blay, F, Ballmer-Weber, B, Fritsche, P, Rivas, M Fernandez, Reig, I, Sinaniotis, A, Vassilopoulou, E, Hoffmann-Sommergruber, K, Vieths, S, Rigby, N, Mills, C, Adel-Patient, K, Blanc, F, Bernard, H, Ah-Leung, S, Przybylski-Nicaise, L, Skov, P Stahl, Purohit, A, de Blay, F, Ballmer-Weber, B, Fritsche, P, Rivas, M Fernandez, Reig, I, Sinaniotis, A, Vassilopoulou, E, Hoffmann-Sommergruber, K, Vieths, S, Rigby, N, Mills, C, and Adel-Patient, K

Abstract

BACKGROUND Sensitization to hazelnut allergens vary depending on the geographic origin and age of the patients. The objective of this study was to further investigate the allergenic activity of hazelnut allergens using sera from patients recruited in various European regions and presenting different sensitization patterns to hazelnut proteins. METHODS Natural Cor a 11 and Cor a 9 were purified from hazelnut whereas Cor a 1 and Cor a 8 were produced as recombinant proteins (rCor a 1.04 and rCor a 8). Sera from hazelnut allergic patients were collected in France (n = 5), Switzerland (n = 2), Greece (n = 11) and Spain (n = 3), within the Europrevall project. Total and allergen-specific IgE were quantified by enzyme allergosorbent test and IgE immunoblot were performed using pooled sera from birch-pollen endemic region or from Greece. Histamine Release (HR) assays were performed with stripped basophils passively sensitized with individual sera and challenged by a hazelnut extract or the different hazelnut allergens. RESULTS As previously described, hazelnut allergic patients from Mediterranean countries are mainly sensitized to the nsLTP Cor a 8 whereas patients from France and Switzerland are sensitized to pollen-related allergens. Interestingly, an intermediate profile was evidenced in patients from Madrid. Hazelnut 7S globulin (Cor a 11) and 11S globulin (Cor a 9) were found to be minor allergens, recognized only by patients from Mediterranean countries. The biologic activity of the 4 tested allergens, analysed by HR assay, further confirmed the sensitization patterns, but also demonstrated the very high elicitation potency of Cor a 8. CONCLUSIONS This work, extending previously published researches, represents a step towards the better understanding of the complexity of hazelnut allergy and provides new data on the biological activity of hazelnut allergens and extracts.

Published
2015

24. Molecular Characterization of Recombinant Mus a 5 Allergen from Banana Fruit

Author

Mrkić, Ivan, Abughren, Mohamed, Nikolić, Jasna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Petersen, Arnd, Papadopoulos, Nikolaos G., Gavrović-Jankulović, Marija, Mrkić, Ivan, Abughren, Mohamed, Nikolić, Jasna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Petersen, Arnd, Papadopoulos, Nikolaos G., and Gavrović-Jankulović, Marija

Abstract

Allergy to banana fruit appears to have become an important cause of fruit allergy in Europe. Among five allergens that have been found, beta-1,3-glucanase denoted as Mus a 5 was identified as a candidate allergen for the component-resolved allergy diagnosis of banana allergy. Because of the variations in protein levels in banana fruit, in this study Mus a 5 was produced as a fusion protein with glutathione-S-transferase in Escherichia coli. The recombinant Mus a 5 was purified under native conditions by a combination of affinity, ion-exchange, and reversed phase chromatography. N-terminal sequence was confirmed by Edman degradation and 55 % of the primary structure was identified by mass fingerprint, while the secondary structure was assessed by circular dichroism spectroscopy. IgG reactivity of recombinant protein was shown in 2-D immunoblot with anti-Mus a 5 antibodies, while IgG and IgE binding to natural Mus a 5 was inhibited with the recombinant Mus a 5 in immunoblot inhibition test. IgE reactivity of recombinant Mus a 5 was shown in ELISA within a group of ten persons sensitized to banana fruit. Recombinant Mus a 5 is a novel reagent suitable for the component-resolved allergy diagnosis of banana allergy.

Published
2014

25. Molecular Characterization of Recombinant Mus a 5 Allergen from Banana Fruit

Author

Mrkić, Ivan, Abughren, Mohamed, Nikolić, Jasna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Petersen, Arnd, Papadopoulos, Nikolaos G., Gavrović-Jankulović, Marija, Mrkić, Ivan, Abughren, Mohamed, Nikolić, Jasna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Petersen, Arnd, Papadopoulos, Nikolaos G., and Gavrović-Jankulović, Marija

Abstract

Allergy to banana fruit appears to have become an important cause of fruit allergy in Europe. Among five allergens that have been found, beta-1,3-glucanase denoted as Mus a 5 was identified as a candidate allergen for the component-resolved allergy diagnosis of banana allergy. Because of the variations in protein levels in banana fruit, in this study Mus a 5 was produced as a fusion protein with glutathione-S-transferase in Escherichia coli. The recombinant Mus a 5 was purified under native conditions by a combination of affinity, ion-exchange, and reversed phase chromatography. N-terminal sequence was confirmed by Edman degradation and 55 % of the primary structure was identified by mass fingerprint, while the secondary structure was assessed by circular dichroism spectroscopy. IgG reactivity of recombinant protein was shown in 2-D immunoblot with anti-Mus a 5 antibodies, while IgG and IgE binding to natural Mus a 5 was inhibited with the recombinant Mus a 5 in immunoblot inhibition test. IgE reactivity of recombinant Mus a 5 was shown in ELISA within a group of ten persons sensitized to banana fruit. Recombinant Mus a 5 is a novel reagent suitable for the component-resolved allergy diagnosis of banana allergy.

Published
2014

26. Molecular Characterization of Recombinant Mus a 5 Allergen from Banana Fruit

Author

Mrkić, Ivan, Abughren, Mohamed, Nikolić, Jasna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Petersen, Arnd, Papadopoulos, Nikolaos G., Gavrović-Jankulović, Marija, Mrkić, Ivan, Abughren, Mohamed, Nikolić, Jasna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Petersen, Arnd, Papadopoulos, Nikolaos G., and Gavrović-Jankulović, Marija

Abstract

Allergy to banana fruit appears to have become an important cause of fruit allergy in Europe. Among five allergens that have been found, beta-1,3-glucanase denoted as Mus a 5 was identified as a candidate allergen for the component-resolved allergy diagnosis of banana allergy. Because of the variations in protein levels in banana fruit, in this study Mus a 5 was produced as a fusion protein with glutathione-S-transferase in Escherichia coli. The recombinant Mus a 5 was purified under native conditions by a combination of affinity, ion-exchange, and reversed phase chromatography. N-terminal sequence was confirmed by Edman degradation and 55 % of the primary structure was identified by mass fingerprint, while the secondary structure was assessed by circular dichroism spectroscopy. IgG reactivity of recombinant protein was shown in 2-D immunoblot with anti-Mus a 5 antibodies, while IgG and IgE binding to natural Mus a 5 was inhibited with the recombinant Mus a 5 in immunoblot inhibition test. IgE reactivity of recombinant Mus a 5 was shown in ELISA within a group of ten persons sensitized to banana fruit. Recombinant Mus a 5 is a novel reagent suitable for the component-resolved allergy diagnosis of banana allergy.

Published
2014

27. Molecular Characterization of Recombinant Mus a 5 Allergen from Banana Fruit

Author

Mrkić, Ivan, Abughren, Mohamed, Nikolić, Jasna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Petersen, Arnd, Papadopoulos, Nikolaos G., Gavrović-Jankulović, Marija, Mrkić, Ivan, Abughren, Mohamed, Nikolić, Jasna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Petersen, Arnd, Papadopoulos, Nikolaos G., and Gavrović-Jankulović, Marija

Abstract

Allergy to banana fruit appears to have become an important cause of fruit allergy in Europe. Among five allergens that have been found, beta-1,3-glucanase denoted as Mus a 5 was identified as a candidate allergen for the component-resolved allergy diagnosis of banana allergy. Because of the variations in protein levels in banana fruit, in this study Mus a 5 was produced as a fusion protein with glutathione-S-transferase in Escherichia coli. The recombinant Mus a 5 was purified under native conditions by a combination of affinity, ion-exchange, and reversed phase chromatography. N-terminal sequence was confirmed by Edman degradation and 55 % of the primary structure was identified by mass fingerprint, while the secondary structure was assessed by circular dichroism spectroscopy. IgG reactivity of recombinant protein was shown in 2-D immunoblot with anti-Mus a 5 antibodies, while IgG and IgE binding to natural Mus a 5 was inhibited with the recombinant Mus a 5 in immunoblot inhibition test. IgE reactivity of recombinant Mus a 5 was shown in ELISA within a group of ten persons sensitized to banana fruit. Recombinant Mus a 5 is a novel reagent suitable for the component-resolved allergy diagnosis of banana allergy.

Published
2014

28. Molecular Characterization of Recombinant Mus a 5 Allergen from Banana Fruit

Author

Mrkić, Ivan, Abughren, Mohamed, Nikolić, Jasna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Petersen, Arnd, Papadopoulos, Nikolaos G., Gavrović-Jankulović, Marija, Mrkić, Ivan, Abughren, Mohamed, Nikolić, Jasna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Petersen, Arnd, Papadopoulos, Nikolaos G., and Gavrović-Jankulović, Marija

Abstract

Allergy to banana fruit appears to have become an important cause of fruit allergy in Europe. Among five allergens that have been found, beta-1,3-glucanase denoted as Mus a 5 was identified as a candidate allergen for the component-resolved allergy diagnosis of banana allergy. Because of the variations in protein levels in banana fruit, in this study Mus a 5 was produced as a fusion protein with glutathione-S-transferase in Escherichia coli. The recombinant Mus a 5 was purified under native conditions by a combination of affinity, ion-exchange, and reversed phase chromatography. N-terminal sequence was confirmed by Edman degradation and 55 % of the primary structure was identified by mass fingerprint, while the secondary structure was assessed by circular dichroism spectroscopy. IgG reactivity of recombinant protein was shown in 2-D immunoblot with anti-Mus a 5 antibodies, while IgG and IgE binding to natural Mus a 5 was inhibited with the recombinant Mus a 5 in immunoblot inhibition test. IgE reactivity of recombinant Mus a 5 was shown in ELISA within a group of ten persons sensitized to banana fruit. Recombinant Mus a 5 is a novel reagent suitable for the component-resolved allergy diagnosis of banana allergy.

Published
2014

29. Molecular Characterization of Recombinant Mus a 5 Allergen from Banana Fruit

Author

Mrkić, Ivan, Abughren, Mohamed, Nikolić, Jasna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Petersen, Arnd, Papadopoulos, Nikolaos G., Gavrović-Jankulović, Marija, Mrkić, Ivan, Abughren, Mohamed, Nikolić, Jasna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Petersen, Arnd, Papadopoulos, Nikolaos G., and Gavrović-Jankulović, Marija

Abstract

Allergy to banana fruit appears to have become an important cause of fruit allergy in Europe. Among five allergens that have been found, beta-1,3-glucanase denoted as Mus a 5 was identified as a candidate allergen for the component-resolved allergy diagnosis of banana allergy. Because of the variations in protein levels in banana fruit, in this study Mus a 5 was produced as a fusion protein with glutathione-S-transferase in Escherichia coli. The recombinant Mus a 5 was purified under native conditions by a combination of affinity, ion-exchange, and reversed phase chromatography. N-terminal sequence was confirmed by Edman degradation and 55 % of the primary structure was identified by mass fingerprint, while the secondary structure was assessed by circular dichroism spectroscopy. IgG reactivity of recombinant protein was shown in 2-D immunoblot with anti-Mus a 5 antibodies, while IgG and IgE binding to natural Mus a 5 was inhibited with the recombinant Mus a 5 in immunoblot inhibition test. IgE reactivity of recombinant Mus a 5 was shown in ELISA within a group of ten persons sensitized to banana fruit. Recombinant Mus a 5 is a novel reagent suitable for the component-resolved allergy diagnosis of banana allergy.

Published
2014

30. Molecular and immunological characterization of Mus a 5 allergen from banana fruit

Author

Aleksić, Ivana, Popović, Milica, Dimitrijević, Rajna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Atanasković-Marković, Marina, Lindner, Buko, Petersen, Arnd, Papadopoulos, Nikolaos G., Gavrović-Jankulović, Marija, Aleksić, Ivana, Popović, Milica, Dimitrijević, Rajna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Atanasković-Marković, Marina, Lindner, Buko, Petersen, Arnd, Papadopoulos, Nikolaos G., and Gavrović-Jankulović, Marija

Abstract

Scope Banana fruit has become an important cause of fruit allergy in the recent years. Among the five registered IUIS allergens, Mus a 1 and Mus a 2 have been characterized in detail. In this study, molecular characterization and evaluation of the allergenic properties of beta-1,3-glucanase from banana (Musa acuminata), denoted as Mus a 5, were performed Methods and results: The gene of Mus a 5 was cloned and sequenced. The obtained cDNA revealed a novel Mus a 5 isoform with an open reading frame encoding a protein of 340 amino acids comprising a putative signal peptide of 28 amino acid residues. By MALDI-TOF analysis Mus a 5 isolated from banana fruit revealed a molecular mass of 33 451 +/- 67 Da. Two Mus a 5 isoforms (pI 7.7 and 8.0) were detected by 2D immunoblot with an identical N-terminal sequence. By mass fingerprint, 76 and 83% of the primary structure was confirmed for the two mature Mus a 5 isoforms, respectively. IgE reactivity to Mus a 5 was found in 74% of patients sensitized to banana fruit. Upregulation of basophil activation markers CD63 and CD203c was achieved with Mus a 5 in a concentration-dependent manner. Conclusion: Mus a 5 is a functional allergen and a candidate for the component-resolved allergy diagnosis of banana allergy.

Published
2012

31. Molecular and immunological characterization of Mus a 5 allergen from banana fruit

Author

Aleksić, Ivana, Popović, Milica, Dimitrijević, Rajna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Atanasković-Marković, Marina, Lindner, Buko, Petersen, Arnd, Papadopoulos, Nikolaos G., Gavrović-Jankulović, Marija, Aleksić, Ivana, Popović, Milica, Dimitrijević, Rajna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Atanasković-Marković, Marina, Lindner, Buko, Petersen, Arnd, Papadopoulos, Nikolaos G., and Gavrović-Jankulović, Marija

Abstract

Scope Banana fruit has become an important cause of fruit allergy in the recent years. Among the five registered IUIS allergens, Mus a 1 and Mus a 2 have been characterized in detail. In this study, molecular characterization and evaluation of the allergenic properties of beta-1,3-glucanase from banana (Musa acuminata), denoted as Mus a 5, were performed Methods and results: The gene of Mus a 5 was cloned and sequenced. The obtained cDNA revealed a novel Mus a 5 isoform with an open reading frame encoding a protein of 340 amino acids comprising a putative signal peptide of 28 amino acid residues. By MALDI-TOF analysis Mus a 5 isolated from banana fruit revealed a molecular mass of 33 451 +/- 67 Da. Two Mus a 5 isoforms (pI 7.7 and 8.0) were detected by 2D immunoblot with an identical N-terminal sequence. By mass fingerprint, 76 and 83% of the primary structure was confirmed for the two mature Mus a 5 isoforms, respectively. IgE reactivity to Mus a 5 was found in 74% of patients sensitized to banana fruit. Upregulation of basophil activation markers CD63 and CD203c was achieved with Mus a 5 in a concentration-dependent manner. Conclusion: Mus a 5 is a functional allergen and a candidate for the component-resolved allergy diagnosis of banana allergy.

Published
2012

32. Molecular and immunological characterization of Mus a 5 allergen from banana fruit

Author

Aleksić, Ivana, Popović, Milica, Dimitrijević, Rajna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Atanasković-Marković, Marina, Lindner, Buko, Petersen, Arnd, Papadopoulos, Nikolaos G., Gavrović-Jankulović, Marija, Aleksić, Ivana, Popović, Milica, Dimitrijević, Rajna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Atanasković-Marković, Marina, Lindner, Buko, Petersen, Arnd, Papadopoulos, Nikolaos G., and Gavrović-Jankulović, Marija

Abstract

Scope Banana fruit has become an important cause of fruit allergy in the recent years. Among the five registered IUIS allergens, Mus a 1 and Mus a 2 have been characterized in detail. In this study, molecular characterization and evaluation of the allergenic properties of beta-1,3-glucanase from banana (Musa acuminata), denoted as Mus a 5, were performed Methods and results: The gene of Mus a 5 was cloned and sequenced. The obtained cDNA revealed a novel Mus a 5 isoform with an open reading frame encoding a protein of 340 amino acids comprising a putative signal peptide of 28 amino acid residues. By MALDI-TOF analysis Mus a 5 isolated from banana fruit revealed a molecular mass of 33 451 +/- 67 Da. Two Mus a 5 isoforms (pI 7.7 and 8.0) were detected by 2D immunoblot with an identical N-terminal sequence. By mass fingerprint, 76 and 83% of the primary structure was confirmed for the two mature Mus a 5 isoforms, respectively. IgE reactivity to Mus a 5 was found in 74% of patients sensitized to banana fruit. Upregulation of basophil activation markers CD63 and CD203c was achieved with Mus a 5 in a concentration-dependent manner. Conclusion: Mus a 5 is a functional allergen and a candidate for the component-resolved allergy diagnosis of banana allergy.

Published
2012

33. Molecular and immunological characterization of Mus a 5 allergen from banana fruit

Author

Aleksić, Ivana, Popović, Milica M., Dimitrijevic, Rajna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Atanasković-Marković, Marina, Lindner, Buko, Petersen, Arnd, Papadopoulos, Nikolaos G., Gavrović-Jankulović, Marija, Aleksić, Ivana, Popović, Milica M., Dimitrijevic, Rajna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Atanasković-Marković, Marina, Lindner, Buko, Petersen, Arnd, Papadopoulos, Nikolaos G., and Gavrović-Jankulović, Marija

Abstract

Scope Banana fruit has become an important cause of fruit allergy in the recent years. Among the five registered IUIS allergens, Mus a 1 and Mus a 2 have been characterized in detail. In this study, molecular characterization and evaluation of the allergenic properties of beta-1,3-glucanase from banana (Musa acuminata), denoted as Mus a 5, were performed Methods and results: The gene of Mus a 5 was cloned and sequenced. The obtained cDNA revealed a novel Mus a 5 isoform with an open reading frame encoding a protein of 340 amino acids comprising a putative signal peptide of 28 amino acid residues. By MALDI-TOF analysis Mus a 5 isolated from banana fruit revealed a molecular mass of 33 451 +/- 67 Da. Two Mus a 5 isoforms (pI 7.7 and 8.0) were detected by 2D immunoblot with an identical N-terminal sequence. By mass fingerprint, 76 and 83% of the primary structure was confirmed for the two mature Mus a 5 isoforms, respectively. IgE reactivity to Mus a 5 was found in 74% of patients sensitized to banana fruit. Upregulation of basophil activation markers CD63 and CD203c was achieved with Mus a 5 in a concentration-dependent manner. Conclusion: Mus a 5 is a functional allergen and a candidate for the component-resolved allergy diagnosis of banana allergy.

Published
2012

34. Molecular and immunological characterization of Mus a 5 allergen from banana fruit

Author

Aleksić, Ivana, Popović, Milica M., Dimitrijevic, Rajna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Atanasković-Marković, Marina, Lindner, Buko, Petersen, Arnd, Papadopoulos, Nikolaos G., Gavrović-Jankulović, Marija, Aleksić, Ivana, Popović, Milica M., Dimitrijevic, Rajna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Atanasković-Marković, Marina, Lindner, Buko, Petersen, Arnd, Papadopoulos, Nikolaos G., and Gavrović-Jankulović, Marija

Abstract

Scope Banana fruit has become an important cause of fruit allergy in the recent years. Among the five registered IUIS allergens, Mus a 1 and Mus a 2 have been characterized in detail. In this study, molecular characterization and evaluation of the allergenic properties of beta-1,3-glucanase from banana (Musa acuminata), denoted as Mus a 5, were performed Methods and results: The gene of Mus a 5 was cloned and sequenced. The obtained cDNA revealed a novel Mus a 5 isoform with an open reading frame encoding a protein of 340 amino acids comprising a putative signal peptide of 28 amino acid residues. By MALDI-TOF analysis Mus a 5 isolated from banana fruit revealed a molecular mass of 33 451 +/- 67 Da. Two Mus a 5 isoforms (pI 7.7 and 8.0) were detected by 2D immunoblot with an identical N-terminal sequence. By mass fingerprint, 76 and 83% of the primary structure was confirmed for the two mature Mus a 5 isoforms, respectively. IgE reactivity to Mus a 5 was found in 74% of patients sensitized to banana fruit. Upregulation of basophil activation markers CD63 and CD203c was achieved with Mus a 5 in a concentration-dependent manner. Conclusion: Mus a 5 is a functional allergen and a candidate for the component-resolved allergy diagnosis of banana allergy.

Published
2012

35. Molecular and immunological characterization of Mus a 5 allergen from banana fruit

Author

Aleksić, Ivana, Popović, Milica, Dimitrijević, Rajna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Atanasković-Marković, Marina, Lindner, Buko, Petersen, Arnd, Papadopoulos, Nikolaos G., Gavrović-Jankulović, Marija, Aleksić, Ivana, Popović, Milica, Dimitrijević, Rajna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Atanasković-Marković, Marina, Lindner, Buko, Petersen, Arnd, Papadopoulos, Nikolaos G., and Gavrović-Jankulović, Marija

Abstract

Scope Banana fruit has become an important cause of fruit allergy in the recent years. Among the five registered IUIS allergens, Mus a 1 and Mus a 2 have been characterized in detail. In this study, molecular characterization and evaluation of the allergenic properties of beta-1,3-glucanase from banana (Musa acuminata), denoted as Mus a 5, were performed Methods and results: The gene of Mus a 5 was cloned and sequenced. The obtained cDNA revealed a novel Mus a 5 isoform with an open reading frame encoding a protein of 340 amino acids comprising a putative signal peptide of 28 amino acid residues. By MALDI-TOF analysis Mus a 5 isolated from banana fruit revealed a molecular mass of 33 451 +/- 67 Da. Two Mus a 5 isoforms (pI 7.7 and 8.0) were detected by 2D immunoblot with an identical N-terminal sequence. By mass fingerprint, 76 and 83% of the primary structure was confirmed for the two mature Mus a 5 isoforms, respectively. IgE reactivity to Mus a 5 was found in 74% of patients sensitized to banana fruit. Upregulation of basophil activation markers CD63 and CD203c was achieved with Mus a 5 in a concentration-dependent manner. Conclusion: Mus a 5 is a functional allergen and a candidate for the component-resolved allergy diagnosis of banana allergy.

Published
2012

36. Molecular and immunological characterization of Mus a 5 allergen from banana fruit

Author

Aleksić, Ivana, Popović, Milica, Dimitrijević, Rajna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Atanasković-Marković, Marina, Lindner, Buko, Petersen, Arnd, Papadopoulos, Nikolaos G., Gavrović-Jankulović, Marija, Aleksić, Ivana, Popović, Milica, Dimitrijević, Rajna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Atanasković-Marković, Marina, Lindner, Buko, Petersen, Arnd, Papadopoulos, Nikolaos G., and Gavrović-Jankulović, Marija

Abstract

Scope Banana fruit has become an important cause of fruit allergy in the recent years. Among the five registered IUIS allergens, Mus a 1 and Mus a 2 have been characterized in detail. In this study, molecular characterization and evaluation of the allergenic properties of beta-1,3-glucanase from banana (Musa acuminata), denoted as Mus a 5, were performed Methods and results: The gene of Mus a 5 was cloned and sequenced. The obtained cDNA revealed a novel Mus a 5 isoform with an open reading frame encoding a protein of 340 amino acids comprising a putative signal peptide of 28 amino acid residues. By MALDI-TOF analysis Mus a 5 isolated from banana fruit revealed a molecular mass of 33 451 +/- 67 Da. Two Mus a 5 isoforms (pI 7.7 and 8.0) were detected by 2D immunoblot with an identical N-terminal sequence. By mass fingerprint, 76 and 83% of the primary structure was confirmed for the two mature Mus a 5 isoforms, respectively. IgE reactivity to Mus a 5 was found in 74% of patients sensitized to banana fruit. Upregulation of basophil activation markers CD63 and CD203c was achieved with Mus a 5 in a concentration-dependent manner. Conclusion: Mus a 5 is a functional allergen and a candidate for the component-resolved allergy diagnosis of banana allergy.

Published
2012

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