1. Single-cell spatial transcriptome reveals cell-type organization in the macaque cortex
- Author
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Chen, Ao, Sun, Yidi, Lei, Ying, Li, Chao, Liao, Sha, Meng, Juan, Bai, Yiqin, Liu, Zhen, Liang, Zhifeng, Zhu, Zhiyong, Yuan, Nini, Yang, Hao, Wu, Zihan, Lin, Feng, Wang, Kexin, Li, Mei, Zhang, Shuzhen, Yang, Meisong, Fei, Tianyi, Zhuang, Zhenkun, Huang, Yiming, Zhang, Yong, Xu, Yuanfang, Cui, Luman, Zhang, Ruiyi, Han, Lei, Sun, Xing, Chen, Bichao, Li, Wenjiao, Huangfu, Baoqian, Ma, Kailong, Ma, Jianyun, Li, Zhao, Lin, Yikun, Wang, He, Zhong, Yanqing, Zhang, Huifang, Yu, Qian, Wang, Yaqian, Liu, Xing, Peng, Jian, Liu, Chuanyu, Chen, Wei, Pan, Wentao, An, Yingjie, Xia, Shihui, Lu, Yanbing, Wang, Mingli, Song, Xinxiang, Liu, Shuai, Wang, Zhifeng, Gong, Chun, Huang, Xin, Yuan, Yue, Zhao, Yun, Chai, Qinwen, Tan, Xing, Liu, Jianfeng, Zheng, Mingyuan, Li, Shengkang, Huang, Yaling, Hong, Yan, Huang, Zirui, Li, Min, Jin, Mengmeng, Li, Yan, Zhang, Hui, Sun, Suhong, Gao, Li, Bai, Yinqi, Cheng, Mengnan, Hu, Guohai, Liu, Shiping, Wang, Bo, Xiang, Bin, Li, Shuting, Li, Huanhuan, Chen, Mengni, Wang, Shiwen, Li, Minglong, Liu, Weibin, Liu, Xin, Zhao, Qian, Lisby, Michael, Wang, Jing, Fang, Jiao, Lin, Yun, Xie, Qing, He, Jie, Xu, Huatai, Huang, Wei, Mulder, Jan, Yang, Huanming, Sun, Yangang, Uhlen, Mathias, Poo, Muming, Wang, Jian, Yao, Jianhua, Wei, Wu, Li, Yuxiang, Shen, Zhiming, Liu, Longqi, Liu, Zhiyong, Xu, Xun, Li, Chengyu, Chen, Ao, Sun, Yidi, Lei, Ying, Li, Chao, Liao, Sha, Meng, Juan, Bai, Yiqin, Liu, Zhen, Liang, Zhifeng, Zhu, Zhiyong, Yuan, Nini, Yang, Hao, Wu, Zihan, Lin, Feng, Wang, Kexin, Li, Mei, Zhang, Shuzhen, Yang, Meisong, Fei, Tianyi, Zhuang, Zhenkun, Huang, Yiming, Zhang, Yong, Xu, Yuanfang, Cui, Luman, Zhang, Ruiyi, Han, Lei, Sun, Xing, Chen, Bichao, Li, Wenjiao, Huangfu, Baoqian, Ma, Kailong, Ma, Jianyun, Li, Zhao, Lin, Yikun, Wang, He, Zhong, Yanqing, Zhang, Huifang, Yu, Qian, Wang, Yaqian, Liu, Xing, Peng, Jian, Liu, Chuanyu, Chen, Wei, Pan, Wentao, An, Yingjie, Xia, Shihui, Lu, Yanbing, Wang, Mingli, Song, Xinxiang, Liu, Shuai, Wang, Zhifeng, Gong, Chun, Huang, Xin, Yuan, Yue, Zhao, Yun, Chai, Qinwen, Tan, Xing, Liu, Jianfeng, Zheng, Mingyuan, Li, Shengkang, Huang, Yaling, Hong, Yan, Huang, Zirui, Li, Min, Jin, Mengmeng, Li, Yan, Zhang, Hui, Sun, Suhong, Gao, Li, Bai, Yinqi, Cheng, Mengnan, Hu, Guohai, Liu, Shiping, Wang, Bo, Xiang, Bin, Li, Shuting, Li, Huanhuan, Chen, Mengni, Wang, Shiwen, Li, Minglong, Liu, Weibin, Liu, Xin, Zhao, Qian, Lisby, Michael, Wang, Jing, Fang, Jiao, Lin, Yun, Xie, Qing, He, Jie, Xu, Huatai, Huang, Wei, Mulder, Jan, Yang, Huanming, Sun, Yangang, Uhlen, Mathias, Poo, Muming, Wang, Jian, Yao, Jianhua, Wei, Wu, Li, Yuxiang, Shen, Zhiming, Liu, Longqi, Liu, Zhiyong, Xu, Xun, and Li, Chengyu
- Abstract
Elucidating the cellular organization of the cerebral cortex is critical for understanding brain structure and function. Using large-scale single-nucleus RNA sequencing and spatial transcriptomic analysis of 143 macaque cortical regions, we obtained a comprehensive atlas of 264 transcriptome-defined cortical cell types and mapped their spatial distribution across the entire cortex. We characterized the cortical layer and region preferences of glutamatergic, GABAergic, and non-neuronal cell types, as well as regional differences in cell-type composition and neighborhood complexity. Notably, we discovered a relationship between the regional distribution of various cell types and the region’s hierarchical level in the visual and somatosensory systems. Cross-species comparison of transcriptomic data from human, macaque, and mouse cortices further revealed primate-specific cell types that are enriched in layer 4, with their marker genes expressed in a region-dependent manner. Our data provide a cellular and molecular basis for understanding the evolution, development, aging, and pathogenesis of the primate brain., Elucidating the cellular organization of the cerebral cortex is critical for understanding brain structure and function. Using large-scale single-nucleus RNA sequencing and spatial transcriptomic analysis of 143 macaque cortical regions, we obtained a comprehensive atlas of 264 transcriptome-defined cortical cell types and mapped their spatial distribution across the entire cortex. We characterized the cortical layer and region preferences of glutamatergic, GABAergic, and non-neuronal cell types, as well as regional differences in cell-type composition and neighborhood complexity. Notably, we discovered a relationship between the regional distribution of various cell types and the region's hierarchical level in the visual and somatosensory systems. Cross-species comparison of transcriptomic data from human, macaque, and mouse cortices further revealed primate-specific cell types that are enriched in layer 4, with their marker genes expressed in a region-dependent manner. Our data provide a cellular and molecular basis for understanding the evolution, development, aging, and pathogenesis of the primate brain.
- Published
- 2023