Author: "Schulz, Sebastian" / Database: OAIster - Searchworks@Jio Institute Digital Library Search Results

Your search keyword '"Schulz, Sebastian"' showing total 33 results

Start Over Author "Schulz, Sebastian" Database OAIster

33 results on '"Schulz, Sebastian"'

1. A Safe Bayesian Optimization Algorithm for Tuning the Optical Synchronization System at European XFEL

Author: Lübsen, Jannis, Schütte, Maximilian, Schulz, Sebastian, Eichler, Annika, Lübsen, Jannis, Schütte, Maximilian, Schulz, Sebastian, and Eichler, Annika
Abstract: The European XFEL is one of the largest linear particle accelerator in the world used to generate extremely intense and ultra short X-Ray flashes to study ultra fast, time resolved chemical processes. In order to improve the quality of the observations, the laser-based optical synchronization system is optimized by tuning PI controllers using a safe Bayesian optimization approach. Since machine time on the European XFEL is very expensive, the algorithm needs to find the optimal parameters as fast as possible. In this contribution, we present a safe Bayesian optimization algorithm which, while guaranteeing safety, shows significantly improved convergence speed and noise robustness. Application and comparison results are presented in simulation for the optical synchronization system of the European XFEL and an experimental demonstration is performed for a laboratory synchronization system.
Published: 2024

2. A case study for measuring the relativistic dipole of a galaxy cross-correlation with the Dark Energy Spectroscopic Instrument

Author: Bonvin, Camille, Lepori, Francesca, Schulz, Sebastian, Tutusaus, Isaac, Adamek, Julian, Fosalba, Pablo, Bonvin, Camille, Lepori, Francesca, Schulz, Sebastian, Tutusaus, Isaac, Adamek, Julian, and Fosalba, Pablo
Abstract: The data on spectroscopic galaxy clustering collected by the Dark Energy Spectroscopic Instrument (DESI) will allow the significant detection of subtle features in the galaxy two-point correlation in redshift space, beyond the "standard" redshift-space distortions. Here we present an independent assessment of the detectability of the relativistic dipole in the cross-correlation of two populations of galaxies if they would be selected from the Bright Galaxy Survey (BGS) of DESI. We build synthetic galaxy catalogues with the characteristics of the BGS using the light cone of a relativistic $N$-body simulation. Exploring different ways of splitting the populations of galaxies we find that with an unequal split with more bright galaxies than faint galaxies the detectability is significantly boosted, reaching 19 $\sigma$ in the redshift bin $0.2 \lesssim z \lesssim 0.3$ and expected to be even higher at lower redshift. Moreover, we find that the measured dipole agrees very well with the prediction of relativistic effects from linear theory down to separations of $\sim$ 30 Mpc/$h$., Comment: version matching publication
Published: 2023
Full Text: View/download PDF

3. Glass-Like Random Catalogues for Two-Point Estimates on the Light Cone

Author: Schulz, Sebastian and Schulz, Sebastian
Abstract: We introduce grlic, a publicly available Python tool for generating glass-like point distributions with a radial density profile $n(r)$ as it is observed in large-scale surveys of galaxy distributions on the past light cone. Utilising these glass-like catalogues, we assess the bias and variance of the Landy-Szalay (LS) estimator of the first three two-point correlation function (2PCF) multipoles in halo and particle catalogues created with the cosmological N-body code gevolution. Our results demonstrate that the LS estimator calculated with the glass catalogues is biased by less than $10^{-4}$ with respect to the estimate derived from Poisson-sampled random catalogues, for all multipoles considered and on all but the smallest scales. Additionally, the estimates derived from glass-like catalogues exhibit significantly smaller standard deviation $\sigma$ than estimates based on commonly used Poisson-sampled random catalogues of comparable size. The standard deviation of the estimate depends on a power of the number of objects $N_R$ in the random catalogue; we find a power law $\sigma \propto N_R^{-0.9}$ for glass-like random catalogues as opposed to $\sigma \propto N_R^{-0.48}$ using Poisson-sampled random catalogues. Given a required precision, this allows for a much reduced number of objects in the glass-like random catalogues used for the LS estimate of the 2PCF multipoles, significantly reducing the computational costs of each estimate., Comment: 16 pages, 14 figures
Published: 2023
Full Text: View/download PDF

4. Euclid: modelling massive neutrinos in cosmology — a code comparison

Author: Adamek, Julian; https://orcid.org/0000-0002-0723-6740, Angulo, Raul E; https://orcid.org/0000-0003-2953-3970, Arnold, Christian, Baldi, Marco; https://orcid.org/0000-0003-4145-1943, Biagetti, Matteo; https://orcid.org/0000-0002-5097-479X, Bose, Ben; https://orcid.org/0000-0003-1965-8614, Carbone, C, Castro, Tiago; https://orcid.org/0000-0002-6292-3228, Dakin, Jeppe; https://orcid.org/0000-0002-2915-0315, Elbers, Willem; https://orcid.org/0000-0002-2207-6108, Potter, Douglas; https://orcid.org/0000-0002-0757-5195, Schneider, Aurel; https://orcid.org/0000-0001-7055-8104, Schulz, Sebastian; https://orcid.org/0000-0002-8235-9986, Stadel, Joachim; https://orcid.org/0000-0001-7565-8622, et al, Adamek, Julian; https://orcid.org/0000-0002-0723-6740, Angulo, Raul E; https://orcid.org/0000-0003-2953-3970, Arnold, Christian, Baldi, Marco; https://orcid.org/0000-0003-4145-1943, Biagetti, Matteo; https://orcid.org/0000-0002-5097-479X, Bose, Ben; https://orcid.org/0000-0003-1965-8614, Carbone, C, Castro, Tiago; https://orcid.org/0000-0002-6292-3228, Dakin, Jeppe; https://orcid.org/0000-0002-2915-0315, Elbers, Willem; https://orcid.org/0000-0002-2207-6108, Potter, Douglas; https://orcid.org/0000-0002-0757-5195, Schneider, Aurel; https://orcid.org/0000-0001-7055-8104, Schulz, Sebastian; https://orcid.org/0000-0002-8235-9986, Stadel, Joachim; https://orcid.org/0000-0001-7565-8622, and et al
Abstract: The measurement of the absolute neutrino mass scale from cosmological large-scale clustering data is one of the key science goals of the Euclid mission. Such a measurement relies on precise modelling of the impact of neutrinos on structure formation, which can be studied with N-body simulations. Here we present the results from a major code comparison effort to establish the maturity and reliability of numerical methods for treating massive neutrinos. The comparison includes eleven full N-body implementations (not all of them independent), two N-body schemes with approximate time integration, and four additional codes that directly predict or emulate the matter power spectrum. Using a common set of initial data we quantify the relative agreement on the nonlinear power spectrum of cold dark matter and baryons and, for the N-body codes, also the relative agreement on the bispectrum, halo mass function, and halo bias. We find that the different numerical implementations produce fully consistent results. We can therefore be confident that we can model the impact of massive neutrinos at the sub-percent level in the most common summary statistics. We also provide a code validation pipeline for future reference.
Published: 2023

5. The halo bias for number counts on the light cone from relativistic N-body simulations

Author: Lepori, Francesca, Schulz, Sebastian; https://orcid.org/0000-0002-8235-9986, Adamek, Julian; https://orcid.org/0000-0002-0723-6740, Durrer, Ruth; https://orcid.org/0000-0001-9833-2086, Lepori, Francesca, Schulz, Sebastian; https://orcid.org/0000-0002-8235-9986, Adamek, Julian; https://orcid.org/0000-0002-0723-6740, and Durrer, Ruth; https://orcid.org/0000-0001-9833-2086
Abstract: We present the halo number counts and its two-point statistics, the observable angular power spectrum, extracted for the first time from relativistic N-body simulations. The halo catalogues used in this work are built from the relativistic N-body code gevolution, and the observed redshift and angular positions of the sources are computed using a non-perturbative ray-tracing method, which includes all relativistic scalar contributions to the number counts. We investigate the validity and limitations of the linear bias prescription to describe our simulated power spectra. In particular, we assess the consistency of different bias measurements on large scales, and we estimate up to which scales a linear bias is accurate in modelling the data, within the statistical errors. We then test a second-order perturbative bias expansion for the angular statistics, on a range of redshifts and scales previously unexplored in this context, that is 0.4 ≤ z̅ ≤ 2 up to scales ℓ $_{max}$ ∼ 1000. We find that the angular power spectra at equal redshift can be modelled with high accuracy with a minimal extension of the number of bias parameters, that is using a two-parameter model comprising linear bias and tidal bias. We show that this model performs significantly better than a model without tidal bias but with quadratic bias as extra degree of freedom, and that the latter is inaccurate at z̅ ≥ 0.7. Finally, we extract from our simulations the cross-correlation of halo number counts and lensing convergence. We show that the estimate of the linear bias from this cross-correlation is consistent with the measurements based on the clustering statistics alone, and that it is crucial to take into account the effect of magnification in the halo number counts to avoid systematic shifts in the computed bias.
Published: 2023

6. Mitochondrial respiration in B lymphocytes is essential for humoral immunity by controlling the flux of the TCA cycle

Author: Urbanczyk, Sophia, Baris, Olivier R., Hofmann, Joerg, Taudte, R. Verena, Guegen, Naig, Golombek, Florian, Castiglione, Kathrin, Meng, Xianyi, Bozec, Aline, Thomas, Jana, Weckwerth, Leonie, Mougiakakos, Dimitrios, Schulz, Sebastian R., Schuh, Wolfgang, Schloetzer-Schrehardt, Ursula, Steinmetz, Tobit D., Brodesser, Susanne, Wiesner, Rudolf J., Mielenz, Dirk, Urbanczyk, Sophia, Baris, Olivier R., Hofmann, Joerg, Taudte, R. Verena, Guegen, Naig, Golombek, Florian, Castiglione, Kathrin, Meng, Xianyi, Bozec, Aline, Thomas, Jana, Weckwerth, Leonie, Mougiakakos, Dimitrios, Schulz, Sebastian R., Schuh, Wolfgang, Schloetzer-Schrehardt, Ursula, Steinmetz, Tobit D., Brodesser, Susanne, Wiesner, Rudolf J., and Mielenz, Dirk
Abstract: To elucidate the function of oxidative phosphorylation (OxPhos) during B cell differentiation, we employ CD23Cre-driven expression of the dominant-negative K320E mutant of the mitochondrial helicase Twinkle (DNT). DNT-expression depletes mitochondrial DNA during B cell maturation, reduces the abundance of respiratory chain protein subunits encoded by mitochondrial DNA, and, consequently, respiratory chain super complexes in activated B cells. Whereas B cell development in DNT mice is normal, B cell proliferation, germinal centers, class switch to IgG, plasma cell maturation, and T cell-dependent as well as T cell -independent humoral immunity are diminished. DNT expression dampens OxPhos but increases glycolysis in lipopolysaccharide and B cell receptor-activated cells. Lipopolysaccharide-activated DNT-B cells exhibit altered metabolites of glycolysis, the pentose phosphate pathway, and the tricarboxylic acid cycle and a lower amount of phosphatidic acid. Consequently, mTORC1 activity and BLIMP1 induction are curtailed, whereas HIF1a is stabilized. Hence, mitochondrial DNA controls the metabolism of activated B cells via OxPhos to foster humoral immunity.
Published: 2022

7. The halo bias for number counts on the light cone from relativistic N-body simulations

Author: Lepori, Francesca, Schulz, Sebastian, Adamek, Julian, Durrer, Ruth, Lepori, Francesca, Schulz, Sebastian, Adamek, Julian, and Durrer, Ruth
Abstract: We present the halo number counts and its two-point statistics, the observable angular power spectrum, extracted for the first time from relativistic N-body simulations. The halo catalogues used in this work are built from the relativistic N-body code gevolution, and the observed redshift and angular positions of the sources are computed using a non-perturbative ray-tracing method, which includes all relativistic scalar contributions to the number counts. We investigate the validity and limitations of the linear bias prescription to describe our simulated power spectra. In particular, we assess the consistency of different bias measurements on large scales, and we estimate up to which scales a linear bias is accurate in modelling the data, within the statistical errors. We then test a second-order perturbative bias expansion for the angular statistics, on a range of redshifts and scales previously unexplored in this context, that is $0.4 \le \bar{z} \le 2$ up to scales $\ell_\mathrm{max} \sim 1000$. We find that the angular power spectra at equal redshift can be modelled with high accuracy with a minimal extension of the number of bias parameters, that is using a two-parameter model comprising linear bias and tidal bias. We show that this model performs significantly better than a model without tidal bias but with quadratic bias as extra degree of freedom, and that the latter is inaccurate at $\bar{z} \ge 0.7$. Finally, we extract from our simulations the cross-correlation of halo number counts and lensing convergence. We show that the estimate of the linear bias from this cross-correlation is consistent with the measurements based on the clustering statistics alone, and that it is crucial to take into account the effect of magnification in the halo number counts to avoid systematic shifts in the computed bias., Comment: 28 pages, 16 figures
Published: 2022
Full Text: View/download PDF

8. The dust-continuum size of TNG50 galaxies at z = 1–5: a comparison with the distribution of stellar light, stars, dust, and H2

Author: MIT Kavli Institute for Astrophysics and Space Research, Massachusetts Institute of Technology. Department of Physics, Popping, Gergö, Pillepich, Annalisa, Calistro Rivera, Gabriela, Schulz, Sebastian, Hernquist, Lars, Kaasinen, Melanie, Marinacci, Federico, Nelson, Dylan, Vogelsberger, Mark, MIT Kavli Institute for Astrophysics and Space Research, Massachusetts Institute of Technology. Department of Physics, Popping, Gergö, Pillepich, Annalisa, Calistro Rivera, Gabriela, Schulz, Sebastian, Hernquist, Lars, Kaasinen, Melanie, Marinacci, Federico, Nelson, Dylan, and Vogelsberger, Mark
Abstract: We present predictions for the extent of the dust-continuum emission of main-sequence galaxies drawn from the TNG50 simulation in the range z = 1–5. We couple the radiative transfer code SKIRT to the output of the TNG50 simulation and measure the dust-continuum half-light radius of the modelled galaxies, assuming a Milky Way dust type and a metallicity-dependent dust-to-metal ratio. The dust-continuum half-light radius at observed-frame 850 $\mu$m is up to ∼75 per cent larger than the stellar half-mass radius, but significantly more compact than the observed-frame 1.6 $\mu$m (roughly corresponding to H band) half-light radius, particularly towards high redshifts: the compactness compared to the 1.6 $\mu$m emission increases with redshift. This is driven by obscuration of stellar light from the galaxy centres, which increases the apparent extent of 1.6 $\mu$m disc sizes relative to that at 850 $\mu$m. The difference in relative extents increases with redshift because the observed-frame 1.6 $\mu$m emission stems from ever shorter wavelength stellar emission. These results suggest that the compact dust-continuum emission observed in z > 1 galaxies is not (necessarily) evidence of the build-up of a dense central stellar component. We find that the dust-continuum half-light radius closely follows the radius containing half the star formation and half the dust mass in galaxies and is ∼80 per cent of the radius containing half the H2 mass. The presented results are a common feature of main-sequence galaxies.
Published: 2022

9. Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

Author: Rolf M. Schwiete Foundation, Associazione Italiana per la Ricerca sul Cancro, German Research Foundation, National Institutes of Health (US), European Commission, Cossarizza, Andrea, Chang, Hyun‐Dong, Radbruch, Andreas, Abrignani, Sergio, Addo, Richard, Akdis, Mübeccel, Andrä, Immanuel, Andreata, Francesco, Annunziato, Francesco, Arranz, Eduardo, Bacher, Petra, Knop, Laura, Knopf, Jasmin, Koay, Hui-Fern, Kobow, Katja, Kriegsmann, Katharina, Kristyanto, H., Krueger, Andreas, Kuehne, Jenny F., Kunze-Schumacher, Heike, Maini, Mala K., Verheyden, Nikita A., Kvistborg, Pia, Kwok, Immanuel, Latorre, Daniela, Mair, Florian, Bari, Sudipto, Manta, Calin, Armin Manz, Rudolf, Mashreghi, Mir-Farzin, Mazzoni, Alessio, McCluskey, James, Borstel, Anouk von, Mei, Henrik E., Melchers, Fritz, Melzer, Susanne, Mielenz, Dirk, Monin, Leticia, Barnaba, Vincenzo, Moretta, Lorenzo, Multhoff, Gabriele, Muñoz, Luis Enrique, Muñoz-Ruiz, Miguel, Ward-Hartstonge, Kirsten A., Muscate, Franziska, Natalini, Ambra, Neumann, Katrin, Guan N., Lai, Niedobitek, Antonia, Niemz, Jana, Barros-Martins, Joana, Nogueira Almeida, Larissa, Notarbartolo, Samuele, Ostendorf, Lennard, Warnatz, Klaus, Pallett, Laura J., Patel, Amit A., Itir Percin, Gulce, Peruzzi, Giovanna, Pinti, Marcello, Pockley, A. Graham, Pracht, Katharina, Baumjohann, Dirk, Prinz, Immo, Pujol-Autonell, Irma, Waskow, Claudia, Pulvirenti, Nadia, Quatrini, Linda, Quinn, Kylie M., Radbruch, Helena, Rhys, Hefin, Rodrigo, Maria B., Romagnani, Chiara, Saggau, Carina, Beccaria, Cristian G., Sakaguchi, Shimon, Wiedemann, Annika, Sallusto, Federica, Sanderink, Lieke, Sandrock, Inga, Schauer, Christine, Scheffold, Alexander, Scherer, Hans U., Schiemann, Matthias, Schildberg, Frank A., Schober, Kilian, Bernardo, David, Wilharm, Anneke, Schoen, Janina, Schuh, Wolfgang, Schüler, Thomas, Schulz, Axel R., Schulz, Sebastian, Schulze, Julia, Simonetti, Sonia, Singh, Jeeshan, Sitnik, Katarzyna M., Stark, Regina, Wing, James, Boardman, Dominic A., Starossom, Sarah, Stehle, Christina, Szelinski, Franziska, Tan, Leonard, Tarnok, Attila, Tornack, Julia, Tree, Timothy I. M., Van Beek, Jasper J. P., Veen, Willem van de, Wirz, Oliver, Gisbergen, Klaas van, Borger, Jessica, Vasco, Chiara, Böttcher, Chotima, Lenz, Daniel, Wittner, Jens, Yang, Jennie H. M., Yang, Juhao, Brockmann, Leonie, Burns, Marie, Busch, Dirk H., Cameron, Garth, Cammarata, Ilenia, Cassotta, Antonino, Chang, Yinshui, Levings, Megan K., Chirdo, Fernando G., Christakou, Eleni, Cicin-Sain, Luka, Cook, Laura, Corbett, Alexandra J., Cornelis, Rebecca, Cosmi, Lorenzo, Davey, Martin S., Biasi, Sara De, Simone, Gabriele De, Lino, Andreia C., Zotto, Genny del, Delacher, Michael, Rosa, Francesca Di, Santo, James Di, Diefenbach, Andreas, Dong, Jun, Dörner, Thomas, Dress, Regine J., Dutertre, Charles-Antoine, Eckle, Sidonia B. G., Liotta, Francesco, Eede, Pascale, Evrard, Maximilien, Falk, Christine S., Feuerer, Markus, Fillatreau, Simon, Fiz-López, Aida, Follo, Marie, Foulds, Gemma A., Fröbel, Julia, Gagliani, Nicola, Long, Heather M., Galletti, Giovanni, Gangaev, Anastasia, Garbi, Natalio, Garrote, José Antonio, Geginat, Jens, Gherardin, Nicholas A., Gibellini, Lara, Ginhoux, Florent, Godfrey, Dale I., Gruarin, Paola, Lugli, Enrico, Haftmann, Claudia, Hansmann, Leo, Harpur, Christopher M., Hayday, Adrian C., Heine, Guido, Hernández, Daniela Carolina, Herrmann, Martin, Hoelsken, Oliver, Huang, Qing, Huber, Samuel, MacDonald, Katherine N., Huber, Johanna E., Huehn, Jochen, Hundemer, Michael, Hwang, William Y. K., Iannacone, Matteo, Ivison, Sabine M., Jäck, Hans-Martin, Jani, Peter K., Keller, Baerbel, Kessler, Nina, Maggi, Laura, Ketelaars, Steven, Rolf M. Schwiete Foundation, Associazione Italiana per la Ricerca sul Cancro, German Research Foundation, National Institutes of Health (US), European Commission, Cossarizza, Andrea, Chang, Hyun‐Dong, Radbruch, Andreas, Abrignani, Sergio, Addo, Richard, Akdis, Mübeccel, Andrä, Immanuel, Andreata, Francesco, Annunziato, Francesco, Arranz, Eduardo, Bacher, Petra, Knop, Laura, Knopf, Jasmin, Koay, Hui-Fern, Kobow, Katja, Kriegsmann, Katharina, Kristyanto, H., Krueger, Andreas, Kuehne, Jenny F., Kunze-Schumacher, Heike, Maini, Mala K., Verheyden, Nikita A., Kvistborg, Pia, Kwok, Immanuel, Latorre, Daniela, Mair, Florian, Bari, Sudipto, Manta, Calin, Armin Manz, Rudolf, Mashreghi, Mir-Farzin, Mazzoni, Alessio, McCluskey, James, Borstel, Anouk von, Mei, Henrik E., Melchers, Fritz, Melzer, Susanne, Mielenz, Dirk, Monin, Leticia, Barnaba, Vincenzo, Moretta, Lorenzo, Multhoff, Gabriele, Muñoz, Luis Enrique, Muñoz-Ruiz, Miguel, Ward-Hartstonge, Kirsten A., Muscate, Franziska, Natalini, Ambra, Neumann, Katrin, Guan N., Lai, Niedobitek, Antonia, Niemz, Jana, Barros-Martins, Joana, Nogueira Almeida, Larissa, Notarbartolo, Samuele, Ostendorf, Lennard, Warnatz, Klaus, Pallett, Laura J., Patel, Amit A., Itir Percin, Gulce, Peruzzi, Giovanna, Pinti, Marcello, Pockley, A. Graham, Pracht, Katharina, Baumjohann, Dirk, Prinz, Immo, Pujol-Autonell, Irma, Waskow, Claudia, Pulvirenti, Nadia, Quatrini, Linda, Quinn, Kylie M., Radbruch, Helena, Rhys, Hefin, Rodrigo, Maria B., Romagnani, Chiara, Saggau, Carina, Beccaria, Cristian G., Sakaguchi, Shimon, Wiedemann, Annika, Sallusto, Federica, Sanderink, Lieke, Sandrock, Inga, Schauer, Christine, Scheffold, Alexander, Scherer, Hans U., Schiemann, Matthias, Schildberg, Frank A., Schober, Kilian, Bernardo, David, Wilharm, Anneke, Schoen, Janina, Schuh, Wolfgang, Schüler, Thomas, Schulz, Axel R., Schulz, Sebastian, Schulze, Julia, Simonetti, Sonia, Singh, Jeeshan, Sitnik, Katarzyna M., Stark, Regina, Wing, James, Boardman, Dominic A., Starossom, Sarah, Stehle, Christina, Szelinski, Franziska, Tan, Leonard, Tarnok, Attila, Tornack, Julia, Tree, Timothy I. M., Van Beek, Jasper J. P., Veen, Willem van de, Wirz, Oliver, Gisbergen, Klaas van, Borger, Jessica, Vasco, Chiara, Böttcher, Chotima, Lenz, Daniel, Wittner, Jens, Yang, Jennie H. M., Yang, Juhao, Brockmann, Leonie, Burns, Marie, Busch, Dirk H., Cameron, Garth, Cammarata, Ilenia, Cassotta, Antonino, Chang, Yinshui, Levings, Megan K., Chirdo, Fernando G., Christakou, Eleni, Cicin-Sain, Luka, Cook, Laura, Corbett, Alexandra J., Cornelis, Rebecca, Cosmi, Lorenzo, Davey, Martin S., Biasi, Sara De, Simone, Gabriele De, Lino, Andreia C., Zotto, Genny del, Delacher, Michael, Rosa, Francesca Di, Santo, James Di, Diefenbach, Andreas, Dong, Jun, Dörner, Thomas, Dress, Regine J., Dutertre, Charles-Antoine, Eckle, Sidonia B. G., Liotta, Francesco, Eede, Pascale, Evrard, Maximilien, Falk, Christine S., Feuerer, Markus, Fillatreau, Simon, Fiz-López, Aida, Follo, Marie, Foulds, Gemma A., Fröbel, Julia, Gagliani, Nicola, Long, Heather M., Galletti, Giovanni, Gangaev, Anastasia, Garbi, Natalio, Garrote, José Antonio, Geginat, Jens, Gherardin, Nicholas A., Gibellini, Lara, Ginhoux, Florent, Godfrey, Dale I., Gruarin, Paola, Lugli, Enrico, Haftmann, Claudia, Hansmann, Leo, Harpur, Christopher M., Hayday, Adrian C., Heine, Guido, Hernández, Daniela Carolina, Herrmann, Martin, Hoelsken, Oliver, Huang, Qing, Huber, Samuel, MacDonald, Katherine N., Huber, Johanna E., Huehn, Jochen, Hundemer, Michael, Hwang, William Y. K., Iannacone, Matteo, Ivison, Sabine M., Jäck, Hans-Martin, Jani, Peter K., Keller, Baerbel, Kessler, Nina, Maggi, Laura, and Ketelaars, Steven
Abstract: The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers.
Published: 2021

10. The dust-continuum size of TNG50 galaxies at $z=1-5$: a comparison with the distribution of stellar light, stars, dust and H$_2$

Author: Popping, Gergö, Pillepich, Annalisa, Rivera, Gabriela Calistro, Schulz, Sebastian, Hernquist, Lars, Kaasinen, Melanie, Marinacci, Federico, Nelson, Dylan, Vogelsberger, Mark, Popping, Gergö, Pillepich, Annalisa, Rivera, Gabriela Calistro, Schulz, Sebastian, Hernquist, Lars, Kaasinen, Melanie, Marinacci, Federico, Nelson, Dylan, and Vogelsberger, Mark
Abstract: We present predictions for the extent of the dust-continuum emission of thousands of main-sequence galaxies drawn from the TNG50 simulation between $z=1-5$. To this aim, we couple the radiative transfer code SKIRT to the output of the TNG50 simulation and measure the dust-continuum half-light radius of the modeled galaxies, assuming a Milky Way dust type and a metallicity dependent dust-to-metal ratio. The dust-continuum half-light radius at observed-frame 850 $\mu$m is up to $\sim$75 per cent larger than the stellar half-mass radius, but significantly more compact than the observed-frame 1.6 $\mu$m (roughly corresponding to H-band) half-light radius, particularly towards high redshifts: the compactness compared to the 1.6 $\mu$m emission increases with redshift. This is driven by obscuration of stellar light from the galaxy centres, which increases the apparent extent of 1.6 $\mu$m disk sizes relative to that at 850 $\mu$m. The difference in relative extents increases with redshift because the observed-frame 1.6 $\mu$m emission stems from ever shorter wavelength stellar emission. These results suggest that the compact dust-continuum emission observed in $z>1$ galaxies is not (necessarily) evidence of the buildup of a dense central stellar component. We also find that the dust-continuum half-light radius very closely follows the radius containing half the star formation in galaxies, indicating that single band dust-continuum emission is a good tracer of the location of (obscured) star formation. The dust-continuum emission is more compact than the H2 mass (for galaxies at $z\geq 2$) and the underlying dust mass. The dust emission strongly correlates with locations with the highest dust temperatures, which do not need to be the locations where most H$_2$ and/or dust is located. The presented results are a common feature of main-sequence galaxies., Comment: Submitted to MNRAS
Published: 2021
Full Text: View/download PDF

11. A pair of noncompeting neutralizing human monoclonal antibodies protecting from disease in a SARS-CoV-2 infection model

Author: Peter, Antonia Sophia, Roth, Edith, Schulz, Sebastian R., Fraedrich, Kirsten, Steinmetz, Tobit, Damm, Dominik, Hauke, Manuela, Richel, Elie, Müller-Schmucker, Sandra, Habenicht, Katharina, Eberlein, Valentina, Issmail, Leila, Uhlig, Nadja, Dolles, Simon, Grüner, Eva, Peterhoff, David, Ciesek, Sandra, Hoffmann, Markus, Pöhlmann, Stefan, McKay, Paul F., Shattock, Robin J., Wölfel, Roman, Socher, Eileen, Wagner, Ralf, Eichler, Jutta, Sticht, Heinrich, Schuh, Wolfgang, Neipel, Frank, Ensser, Armin, Mielenz, Dirk Alexander, Tenbusch, Matthias, Winkler, Thomas H., Grunwald, Thomas, Überla, Klaus Thomas, Jäck, Hans-Martin, Peter, Antonia Sophia, Roth, Edith, Schulz, Sebastian R., Fraedrich, Kirsten, Steinmetz, Tobit, Damm, Dominik, Hauke, Manuela, Richel, Elie, Müller-Schmucker, Sandra, Habenicht, Katharina, Eberlein, Valentina, Issmail, Leila, Uhlig, Nadja, Dolles, Simon, Grüner, Eva, Peterhoff, David, Ciesek, Sandra, Hoffmann, Markus, Pöhlmann, Stefan, McKay, Paul F., Shattock, Robin J., Wölfel, Roman, Socher, Eileen, Wagner, Ralf, Eichler, Jutta, Sticht, Heinrich, Schuh, Wolfgang, Neipel, Frank, Ensser, Armin, Mielenz, Dirk Alexander, Tenbusch, Matthias, Winkler, Thomas H., Grunwald, Thomas, Überla, Klaus Thomas, and Jäck, Hans-Martin
Abstract: TRIANNI mice carry an entire set of human immunoglobulin V region gene segments and are a powerful tool to rapidly isolate human monoclonal antibodies. After immunizing these mice with DNA encoding the spike protein of SARS-CoV-2 and boosting with spike protein, we identified 29 hybridoma antibodies that reacted with the SARS-CoV-2 spike protein. Nine antibodies neutralize SARS-CoV-2 infection at IC50 values in the subnanomolar range. ELISA-binding studies and DNA sequence analyses revealed one cluster of three clonally related neutralizing antibodies that target the receptor-binding domain and compete with the cellular receptor hACE2. A second cluster of six clonally related neutralizing antibodies bind to the N-terminal domain of the spike protein without competing with the binding of hACE2 or cluster 1 antibodies. SARS-CoV-2 mutants selected for resistance to an antibody from one cluster are still neutralized by an antibody from the other cluster. Antibodies from both clusters markedly reduced viral spread in mice transgenic for human ACE2 and protected the animals from SARS-CoV-2-induced weight loss. The two clusters of potent noncompeting SARS-CoV-2 neutralizing antibodies provide potential candidates for therapy and prophylaxis of COVID-19. The study further supports transgenic animals with a human immunoglobulin gene repertoire as a powerful platform in pandemic preparedness initiatives.
Published: 2021

12. Escherichia coli Data-Driven Strain Design Using Aggregated Adaptive Laboratory Evolution Mutational Data

Author: Phaneuf, Patrick V, Zielinski, Daniel C, Yurkovich, James T, Johnsen, Josefin, Szubin, Richard, Yang, Lei, Kim, Se Hyeuk, Schulz, Sebastian, Wu, Muyao, Dalldorf, Christopher, Ozdemir, Emre, Lennen, Rebecca M, Palsson, Bernhard O, Feist, Adam M, Phaneuf, Patrick V, Zielinski, Daniel C, Yurkovich, James T, Johnsen, Josefin, Szubin, Richard, Yang, Lei, Kim, Se Hyeuk, Schulz, Sebastian, Wu, Muyao, Dalldorf, Christopher, Ozdemir, Emre, Lennen, Rebecca M, Palsson, Bernhard O, and Feist, Adam M
Abstract: Microbes are being engineered for an increasingly large and diverse set of applications. However, the designing of microbial genomes remains challenging due to the general complexity of biological systems. Adaptive Laboratory Evolution (ALE) leverages nature's problem-solving processes to generate optimized genotypes currently inaccessible to rational methods. The large amount of public ALE data now represents a new opportunity for data-driven strain design. This study describes how novel strain designs, or genome sequences not yet observed in ALE experiments or published designs, can be extracted from aggregated ALE data and demonstrates this by designing, building, and testing three novel Escherichia coli strains with fitnesses comparable to ALE mutants. These designs were achieved through a meta-analysis of aggregated ALE mutations data (63 Escherichia coli K-12 MG1655 based ALE experiments, described by 93 unique environmental conditions, 357 independent evolutions, and 13 957 observed mutations), which additionally revealed global ALE mutation trends that inform on ALE-derived strain design principles. Such informative trends anticipate ALE-derived strain designs as largely gene-centric, as opposed to noncoding, and composed of a relatively small number of beneficial variants (approximately 6). These results demonstrate how strain design efforts can be enhanced by the meta-analysis of aggregated ALE data.
Published: 2021

13. A redshift-dependent IRX-$\beta$ dust attenuation relation for TNG50 galaxies

Author: Schulz, Sebastian, Popping, Gergö, Pillepich, Annalisa, Nelson, Dylan, Vogelsberger, Mark, Marinacci, Federico, Hernquist, Lars, Schulz, Sebastian, Popping, Gergö, Pillepich, Annalisa, Nelson, Dylan, Vogelsberger, Mark, Marinacci, Federico, and Hernquist, Lars
Abstract: We study the relation between the UV-slope, $\beta$, and the ratio between the infrared- and UV-luminosities (IRX) of galaxies from TNG50, the latest installment of the IllustrisTNG galaxy formation simulations. We select 7280 star-forming main-sequence (SFMS) galaxies with stellar mass $10^9M_\odot$ at redshifts $0 \leq z \leq 4$ and perform radiative transfer with SKIRT to model effects of interstellar medium dust on the emitted stellar light. Assuming a Milky Way (MW) dust type and a dust-to-metal ratio of 0.3, we find that TNG50 SFMS galaxies generally agree with observationally-derived IRX-$\beta$ relations at $z \lesssim 1$. However, we find a redshift-dependent systematic offset with respect to empirically-derived local relations, with the TNG50 IRX-$\beta$ relation shifting towards lower $\beta$ and steppening at higher redshifts. This is partially driven by variations in the dust-uncorrected UV-slope of galaxies, due to different star-formation histories of galaxies selected at different cosmic epochs; we suggest the remainder of the effect is caused by differences in the effective dust attenuation curves (EDACs) of galaxies as a function of redshift. We find a typical galaxy-to-galaxy variation of 0.3 dex in IRX at fixed $\beta$, correlated with intrinsic galaxy properties: galaxies with higher star-formation rates, star-formation efficiencies, gas metallicities and stellar masses exhibit larger IRX values. We demonstrate a degeneracy between stellar age, dust geometry and dust composition: $z=4$ galaxies with a Small Magellanic Cloud dust type follow the same IRX-$\beta$ relation as low-redshift galaxies with MW dust. We provide a redshift-dependent fitting function for the IRX-$\beta$ relation for MW dust based on our models., Comment: The paper has been accepted for publishing in MNRAS
Published: 2020
Full Text: View/download PDF

14. Über die Veränderung der Insulinsensitivität in gesunden, freiwilligen Probanden nach 14-tägiger Einnahme von Tenofovir alafenamid-haltiger antiretroviraler Medikation

Author: Spinner, Christoph (Priv.-Doz. Dr.), Schmid, Roland M. (Prof. Dr.), Schulz, Sebastian Peter, Spinner, Christoph (Priv.-Doz. Dr.), Schmid, Roland M. (Prof. Dr.), and Schulz, Sebastian Peter
Abstract: Die TAF-IR-Studie konnte durch die Messung der Insulinresistenz unter 14-tägiger Einnahme von F/TAF, E/C/F/TAF und R/F/TAF in gesunden Probanden keine negativen Effekte auf den Glukosemetabolismus mittels HEGC finden. Dies unterstreicht die bereits erwartete Patientensicherheit der weiterentwickelten antiretroviralen Medikation mit Tenofovir alafenamid auch im Hinblick auf die Insulinsensitivität. Es ergaben sich daher keine Hinweise auf eine diabetogene Wirkung bei 14-tägiger Einnahme von TAF., The TAF-IR-study clearly demonstrated, for the first time, that healthy volunteers taking F/TAF, E/C/F/TAF or R/F/TAF over a 14-day period did not show any significant nor relevant changes in IR, as assessed by MBW, BBW/I and MCR in HECG. These results support the assumed safety of TAF-based approaches in HIV treatment.
Published: 2020

15. Dr Schulz, Sebastian

Author: Dr Schulz, Sebastian and Dr Schulz, Sebastian
Published: 2020

16. Dr Schulz, Sebastian

Author: Dr Schulz, Sebastian and Dr Schulz, Sebastian
Published: 2020

17. A redshift-dependent IRX-$\beta$ dust attenuation relation for TNG50 galaxies

Author: Schulz, Sebastian, Popping, Gergö, Pillepich, Annalisa, Nelson, Dylan, Vogelsberger, Mark, Marinacci, Federico, Hernquist, Lars, Schulz, Sebastian, Popping, Gergö, Pillepich, Annalisa, Nelson, Dylan, Vogelsberger, Mark, Marinacci, Federico, and Hernquist, Lars
Abstract: We study the relation between the UV-slope, $\beta$, and the ratio between the infrared- and UV-luminosities (IRX) of galaxies from TNG50, the latest installment of the IllustrisTNG galaxy formation simulations. We select 7280 star-forming main-sequence (SFMS) galaxies with stellar mass $10^9M_\odot$ at redshifts $0 \leq z \leq 4$ and perform radiative transfer with SKIRT to model effects of interstellar medium dust on the emitted stellar light. Assuming a Milky Way (MW) dust type and a dust-to-metal ratio of 0.3, we find that TNG50 SFMS galaxies generally agree with observationally-derived IRX-$\beta$ relations at $z \lesssim 1$. However, we find a redshift-dependent systematic offset with respect to empirically-derived local relations, with the TNG50 IRX-$\beta$ relation shifting towards lower $\beta$ and steppening at higher redshifts. This is partially driven by variations in the dust-uncorrected UV-slope of galaxies, due to different star-formation histories of galaxies selected at different cosmic epochs; we suggest the remainder of the effect is caused by differences in the effective dust attenuation curves (EDACs) of galaxies as a function of redshift. We find a typical galaxy-to-galaxy variation of 0.3 dex in IRX at fixed $\beta$, correlated with intrinsic galaxy properties: galaxies with higher star-formation rates, star-formation efficiencies, gas metallicities and stellar masses exhibit larger IRX values. We demonstrate a degeneracy between stellar age, dust geometry and dust composition: $z=4$ galaxies with a Small Magellanic Cloud dust type follow the same IRX-$\beta$ relation as low-redshift galaxies with MW dust. We provide a redshift-dependent fitting function for the IRX-$\beta$ relation for MW dust based on our models., Comment: The paper has been accepted for publishing in MNRAS
Published: 2020
Full Text: View/download PDF

18. Über die Veränderung der Insulinsensitivität in gesunden, freiwilligen Probanden nach 14-tägiger Einnahme von Tenofovir alafenamid-haltiger antiretroviraler Medikation

Author: Schmid, Roland M. (Prof. Dr.), Schmid, Roland M. (Prof. Dr.);Spinner, Christoph (Priv.-Doz. Dr.), Schulz, Sebastian Peter, Schmid, Roland M. (Prof. Dr.), Schmid, Roland M. (Prof. Dr.);Spinner, Christoph (Priv.-Doz. Dr.), and Schulz, Sebastian Peter
Abstract: Die TAF-IR-Studie konnte durch die Messung der Insulinresistenz unter 14-tägiger Einnahme von F/TAF, E/C/F/TAF und R/F/TAF in gesunden Probanden keine negativen Effekte auf den Glukosemetabolismus mittels HEGC finden. Dies unterstreicht die bereits erwartete Patientensicherheit der weiterentwickelten antiretroviralen Medikation mit Tenofovir alafenamid auch im Hinblick auf die Insulinsensitivität. Es ergaben sich daher keine Hinweise auf eine diabetogene Wirkung bei 14-tägiger Einnahme von TAF., The TAF-IR-study clearly demonstrated, for the first time, that healthy volunteers taking F/TAF, E/C/F/TAF or R/F/TAF over a 14-day period did not show any significant nor relevant changes in IR, as assessed by MBW, BBW/I and MCR in HECG. These results support the assumed safety of TAF-based approaches in HIV treatment.
Published: 2020

19. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Author: Cossarizza, Andrea, Chang, Hyun-Dong, Radbruch, Andreas, Acs, Andreas, Adam, Dieter, Adam-Klages, Sabine, Agace, William W., Aghaeepour, Nima, Akdis, Muebeccel, Allez, Matthieu, Almeida, Larissa Nogueira, Alvisi, Giorgia, Anderson, Graham, Andrae, Immanuel, Annunziato, Francesco, Anselmo, Achille, Bacher, Petra, Baldari, Cosima T., Bari, Sudipto, Barnaba, Vincenzo, Barros-Martins, Joana, Battistini, Luca, Bauer, Wolfgang, Baumgart, Sabine, Baumgarth, Nicole, Baumjohann, Dirk, Baying, Bianka, Bebawy, Mary, Becher, Burkhard, Beisker, Wolfgang, Benes, Vladimir, Beyaert, Rudi, Blanco, Alfonso, Boardman, Dominic A., Bogdan, Christian, Borger, Jessica G., Borsellino, Giovanna, Boulais, Philip E., Bradford, Jolene A., Brenner, Dirk, Brinkman, Ryan R., Brooks, Anna E. S., Busch, Dirk H., Buescher, Martin, Bushnell, Timothy P., Calzetti, Federica, Cameron, Garth, Cammarata, Ilenia, Cao, Xuetao, Cardell, Susanna L., Casola, Stefano, Cassatella, Marco A., Cavani, Andrea, Celada, Antonio, Chatenoud, Lucienne, Chattopadhyay, Pratip K., Chow, Sue, Christakou, Eleni, Cicin-Sain, Luka, Clerici, Mario, Colombo, Federico S., Cook, Laura, Cooke, Anne, Cooper, Andrea M., Corbett, Alexandra J., Cosma, Antonio, Cosmi, Lorenzo, Coulie, Pierre G., Cumano, Ana, Cvetkovic, Ljiljana, Dang, Van Duc, Dang-Heine, Chantip, Davey, Martin S., Davies, Derek, De Biasi, Sara, Del Zotto, Genny, Dela Cruz, Gelo Victoriano, Delacher, Michael, Della Bella, Silvia, Dellabona, Paolo, Deniz, Guennur, Dessing, Mark, Di Santo, James P., Diefenbach, Andreas, Dieli, Francesco, Dolf, Andreas, Doerner, Thomas, Dress, Regine J., Dudziak, Diana, Dustin, Michael, Dutertre, Charles-Antoine, Ebner, Friederike, Eckle, Sidonia B. G., Edinger, Matthias, Eede, Pascale, Ehrhardt, Goetz R. A., Eich, Marcus, Engel, Pablo, Engelhardt, Britta, Erdei, Anna, Esser, Charlotte, Everts, Bart, Evrard, Maximilien, Falk, Christine S., Fehniger, Todd A., Felipo-Benavent, Mar, Ferry, Helen, Feuerer, Markus, Filby, Andrew, Filkor, Kata, Fillatreau, Simon, Follo, Marie, Foerster, Irmgard, Foster, John, Foulds, Gemma A., Frehse, Britta, Frenette, Paul S., Frischbutter, Stefan, Fritzsche, Wolfgang, Galbraith, David W., Gangaev, Anastasia, Garbi, Natalio, Gaudilliere, Brice, Gazzinelli, Ricardo T., Geginat, Jens, Gerner, Wilhelm, Gherardin, Nicholas A., Ghoreschi, Kamran, Gibellini, Lara, Ginhoux, Florent, Goda, Keisuke, Godfrey, Dale I., Goettlinger, Christoph, Gonzalez-Navajas, Jose M., Goodyear, Carl S., Gori, Andrea, Grogan, Jane L., Grummitt, Daryl, Gruetzkau, Andreas, Haftmann, Claudia, Hahn, Jonas, Hammad, Hamida, Haemmerling, Guenter, Hansmann, Leo, Hansson, Goran, Harpur, Christopher M., Hartmann, Susanne, Hauser, Andrea, Hauser, Anja E., Haviland, David L., Hedley, David, Hernandez, Daniela C., Herrera, Guadalupe, Herrmann, Martin, Hess, Christoph, Hoefer, Thomas, Hoffmann, Petra, Hogquist, Kristin, Holland, Tristan, Hollt, Thomas, Holmdahl, Rikard, Hombrink, Pleun, Houston, Jessica P., Hoyer, Bimba F., Huang, Bo, Huang, Fang-Ping, Huber, Johanna E., Huehn, Jochen, Hundemer, Michael, Hunter, Christopher A., Hwang, William Y. K., Iannone, Anna, Ingelfinger, Florian, Ivison, Sabine M., Jaeck, Hans-Martin, Jani, Peter K., Javega, Beatriz, Jonjic, Stipan, Kaiser, Toralf, Kalina, Tomas, Kamradt, Thomas, Kaufmann, Stefan H. E., Keller, Baerbel, Ketelaars, Steven L. C., Khalilnezhad, Ahad, Khan, Srijit, Kisielow, Jan, Klenerman, Paul, Knopf, Jasmin, Koay, Hui-Fern, Kobow, Katja, Kolls, Jay K., Kong, Wan Ting, Kopf, Manfred, Korn, Thomas, Kriegsmann, Katharina, Kristyanto, Hendy, Kroneis, Thomas, Krueger, Andreas, Kuehne, Jenny, Kukat, Christian, Kunkel, Desiree, Kunze-Schumacher, Heike, Kurosaki, Tomohiro, Kurts, Christian, Kvistborg, Pia, Kwok, Immanuel, Landry, Jonathan, Lantz, Olivier, Lanuti, Paola, LaRosa, Francesca, Lehuen, Agnes, LeibundGut-Landmann, Salome, Leipold, Michael D., Leung, Leslie Y. T., Levings, Megan K., Lino, Andreia C., Liotta, Francesco, Litwin, Virginia, Liu, Yanling, Ljunggren, Hans-Gustaf, Lohoff, Michael, Lombardi, Giovanna, Lopez, Lilly, Lopez-Botet, Miguel, Lovett-Racke, Amy E., Lubberts, Erik, Luche, Herve, Ludewig, Burkhard, Lugli, Enrico, Lunemann, Sebastian, Maecker, Holden T., Maggi, Laura, Maguire, Orla, Mair, Florian, Mair, Kerstin H., Mantovani, Alberto, Manz, Rudolf A., Marshall, Aaron J., Martinez-Romero, Alicia, Martrus, Gloria, Marventano, Ivana, Maslinski, Wlodzimierz, Matarese, Giuseppe, Mattioli, Anna Vittoria, Maueroder, Christian, Mazzoni, Alessio, McCluskey, James, McGrath, Mairi, McGuire, Helen M., McInnes, Iain B., Mei, Henrik E., Melchers, Fritz, Melzer, Susanne, Mielenz, Dirk, Miller, Stephen D., Mills, Kingston H. G., Minderman, Hans, Mjosberg, Jenny, Moore, Jonni, Moran, Barry, Moretta, Lorenzo, Mosmann, Tim R., Mueller, Susann, Multhoff, Gabriele, Munoz, Luis Enrique, Munz, Christian, Nakayama, Toshinori, Nasi, Milena, Neumann, Katrin, Ng, Lai Guan, Niedobitek, Antonia, Nourshargh, Sussan, Nunez, Gabriel, O'Connor, Jose-Enrique, Ochel, Aaron, Oja, Anna, Ordonez, Diana, Orfao, Alberto, Orlowski-Oliver, Eva, Ouyang, Wenjun, Oxenius, Annette, Palankar, Raghavendra, Panse, Isabel, Pattanapanyasat, Kovit, Paulsen, Malte, Pavlinic, Dinko, Penter, Livius, Peterson, Paert, Peth, Christian, Petriz, Jordi, Piancone, Federica, Pickl, Winfried F., Piconese, Silvia, Pinti, Marcello, Pockley, A. Graham, Podolska, Malgorzata Justyna, Poon, Zhiyong, Pracht, Katharina, Prinz, Immo, Pucillo, Carlo E. M., Quataert, Sally A., Quatrini, Linda, Quinn, Kylie M., Radbruch, Helena, Radstake, Tim R. D. J., Rahmig, Susann, Rahn, Hans-Peter, Rajwa, Bartek, Ravichandran, Gevitha, Raz, Yotam, Rebhahn, Jonathan A., Recktenwald, Diether, Reimer, Dorothea, Reis e Sousa, Caetano, Remmerswaal, Ester B. M., Richter, Lisa, Rico, Laura G., Riddell, Andy, Rieger, Aja M., Robinson, J. Paul, Romagnani, Chiara, Rubartelli, Anna, Ruland, Juergen, Saalmueller, Armin, Saeys, Yvan, Saito, Takashi, Sakaguchi, Shimon, Sala-de-Oyanguren, Francisco, Samstag, Yvonne, Sanderson, Sharon, Sandrock, Inga, Santoni, Angela, Sanz, Ramon Bellmas, Saresella, Marina, Sautes-Fridman, Catherine, Sawitzki, Birgit, Schadt, Linda, Scheffold, Alexander, Scherer, Hans U., Schiemann, Matthias, Schildberg, Frank A., Schimisky, Esther, Schlitzer, Andreas, Schlosser, Josephine, Schmid, Stephan, Schmitt, Steffen, Schober, Kilian, Schraivogel, Daniel, Schuh, Wolfgang, Schueler, Thomas, Schulte, Reiner, Schulz, Axel Ronald, Schulz, Sebastian R., Scotta, Cristiano, Scott-Algara, Daniel, Sester, David P., Shankey, T. Vincent, Silva-Santos, Bruno, Simon, Anna Katharina, Sitnik, Katarzyna M., Sozzani, Silvano, Speiser, Daniel E., Spidlen, Josef, Stahlberg, Anders, Stall, Alan M., Stanley, Natalie, Stark, Regina, Stehle, Christina, Steinmetz, Tobit, Stockinger, Hannes, Takahama, Yousuke, Takeda, Kiyoshi, Tan, Leonard, Tarnok, Attila, Tiegs, Gisa, Toldi, Gergely, Tornack, Julia, Traggiai, Elisabetta, Trebak, Mohamed, Tree, Timothy I. M., Trotter, Joe, Trowsdale, John, Tsoumakidou, Maria, Ulrich, Henning, Urbanczyk, Sophia, van de Veen, Willem, van den Broek, Maries, van der Pol, Edwin, Van Gassen, Sofie, Van Isterdael, Gert, van Lier, Rene A. W., Veldhoen, Marc, Vento-Asturias, Salvador, Vieira, Paulo, Voehringer, David, Volk, Hans-Dieter, von Borstel, Anouk, von Volkmann, Konrad, Waisman, Ari, Walker, Rachael V., Wallace, Paul K., Wang, Sa A., Wang, Xin M., Ward, Michael D., Ward-Hartstonge, Kirsten A., Warnatz, Klaus, Warnes, Gary, Warth, Sarah, Waskow, Claudia, Watson, James V., Watzl, Carsten, Wegener, Leonie, Weisenburger, Thomas, Wiedemann, Annika, Wienands, Juergen, Wilharm, Anneke, Wilkinson, Robert John, Willimsky, Gerald, Wing, James B., Winkelmann, Rieke, Winkler, Thomas H., Wirz, Oliver F., Wong, Alicia, Wurst, Peter, Yang, Jennie H. M., Yang, Juhao, Yazdanbakhsh, Maria, Yu, Liping, Yue, Alice, Zhang, Hanlin, Zhao, Yi, Ziegler, Susanne Maria, Zielinski, Christina, Zimmermann, Jakob, Zychlinsky, Arturo, Cossarizza, Andrea, Chang, Hyun-Dong, Radbruch, Andreas, Acs, Andreas, Adam, Dieter, Adam-Klages, Sabine, Agace, William W., Aghaeepour, Nima, Akdis, Muebeccel, Allez, Matthieu, Almeida, Larissa Nogueira, Alvisi, Giorgia, Anderson, Graham, Andrae, Immanuel, Annunziato, Francesco, Anselmo, Achille, Bacher, Petra, Baldari, Cosima T., Bari, Sudipto, Barnaba, Vincenzo, Barros-Martins, Joana, Battistini, Luca, Bauer, Wolfgang, Baumgart, Sabine, Baumgarth, Nicole, Baumjohann, Dirk, Baying, Bianka, Bebawy, Mary, Becher, Burkhard, Beisker, Wolfgang, Benes, Vladimir, Beyaert, Rudi, Blanco, Alfonso, Boardman, Dominic A., Bogdan, Christian, Borger, Jessica G., Borsellino, Giovanna, Boulais, Philip E., Bradford, Jolene A., Brenner, Dirk, Brinkman, Ryan R., Brooks, Anna E. S., Busch, Dirk H., Buescher, Martin, Bushnell, Timothy P., Calzetti, Federica, Cameron, Garth, Cammarata, Ilenia, Cao, Xuetao, Cardell, Susanna L., Casola, Stefano, Cassatella, Marco A., Cavani, Andrea, Celada, Antonio, Chatenoud, Lucienne, Chattopadhyay, Pratip K., Chow, Sue, Christakou, Eleni, Cicin-Sain, Luka, Clerici, Mario, Colombo, Federico S., Cook, Laura, Cooke, Anne, Cooper, Andrea M., Corbett, Alexandra J., Cosma, Antonio, Cosmi, Lorenzo, Coulie, Pierre G., Cumano, Ana, Cvetkovic, Ljiljana, Dang, Van Duc, Dang-Heine, Chantip, Davey, Martin S., Davies, Derek, De Biasi, Sara, Del Zotto, Genny, Dela Cruz, Gelo Victoriano, Delacher, Michael, Della Bella, Silvia, Dellabona, Paolo, Deniz, Guennur, Dessing, Mark, Di Santo, James P., Diefenbach, Andreas, Dieli, Francesco, Dolf, Andreas, Doerner, Thomas, Dress, Regine J., Dudziak, Diana, Dustin, Michael, Dutertre, Charles-Antoine, Ebner, Friederike, Eckle, Sidonia B. G., Edinger, Matthias, Eede, Pascale, Ehrhardt, Goetz R. A., Eich, Marcus, Engel, Pablo, Engelhardt, Britta, Erdei, Anna, Esser, Charlotte, Everts, Bart, Evrard, Maximilien, Falk, Christine S., Fehniger, Todd A., Felipo-Benavent, Mar, Ferry, Helen, Feuerer, Markus, Filby, Andrew, Filkor, Kata, Fillatreau, Simon, Follo, Marie, Foerster, Irmgard, Foster, John, Foulds, Gemma A., Frehse, Britta, Frenette, Paul S., Frischbutter, Stefan, Fritzsche, Wolfgang, Galbraith, David W., Gangaev, Anastasia, Garbi, Natalio, Gaudilliere, Brice, Gazzinelli, Ricardo T., Geginat, Jens, Gerner, Wilhelm, Gherardin, Nicholas A., Ghoreschi, Kamran, Gibellini, Lara, Ginhoux, Florent, Goda, Keisuke, Godfrey, Dale I., Goettlinger, Christoph, Gonzalez-Navajas, Jose M., Goodyear, Carl S., Gori, Andrea, Grogan, Jane L., Grummitt, Daryl, Gruetzkau, Andreas, Haftmann, Claudia, Hahn, Jonas, Hammad, Hamida, Haemmerling, Guenter, Hansmann, Leo, Hansson, Goran, Harpur, Christopher M., Hartmann, Susanne, Hauser, Andrea, Hauser, Anja E., Haviland, David L., Hedley, David, Hernandez, Daniela C., Herrera, Guadalupe, Herrmann, Martin, Hess, Christoph, Hoefer, Thomas, Hoffmann, Petra, Hogquist, Kristin, Holland, Tristan, Hollt, Thomas, Holmdahl, Rikard, Hombrink, Pleun, Houston, Jessica P., Hoyer, Bimba F., Huang, Bo, Huang, Fang-Ping, Huber, Johanna E., Huehn, Jochen, Hundemer, Michael, Hunter, Christopher A., Hwang, William Y. K., Iannone, Anna, Ingelfinger, Florian, Ivison, Sabine M., Jaeck, Hans-Martin, Jani, Peter K., Javega, Beatriz, Jonjic, Stipan, Kaiser, Toralf, Kalina, Tomas, Kamradt, Thomas, Kaufmann, Stefan H. E., Keller, Baerbel, Ketelaars, Steven L. C., Khalilnezhad, Ahad, Khan, Srijit, Kisielow, Jan, Klenerman, Paul, Knopf, Jasmin, Koay, Hui-Fern, Kobow, Katja, Kolls, Jay K., Kong, Wan Ting, Kopf, Manfred, Korn, Thomas, Kriegsmann, Katharina, Kristyanto, Hendy, Kroneis, Thomas, Krueger, Andreas, Kuehne, Jenny, Kukat, Christian, Kunkel, Desiree, Kunze-Schumacher, Heike, Kurosaki, Tomohiro, Kurts, Christian, Kvistborg, Pia, Kwok, Immanuel, Landry, Jonathan, Lantz, Olivier, Lanuti, Paola, LaRosa, Francesca, Lehuen, Agnes, LeibundGut-Landmann, Salome, Leipold, Michael D., Leung, Leslie Y. T., Levings, Megan K., Lino, Andreia C., Liotta, Francesco, Litwin, Virginia, Liu, Yanling, Ljunggren, Hans-Gustaf, Lohoff, Michael, Lombardi, Giovanna, Lopez, Lilly, Lopez-Botet, Miguel, Lovett-Racke, Amy E., Lubberts, Erik, Luche, Herve, Ludewig, Burkhard, Lugli, Enrico, Lunemann, Sebastian, Maecker, Holden T., Maggi, Laura, Maguire, Orla, Mair, Florian, Mair, Kerstin H., Mantovani, Alberto, Manz, Rudolf A., Marshall, Aaron J., Martinez-Romero, Alicia, Martrus, Gloria, Marventano, Ivana, Maslinski, Wlodzimierz, Matarese, Giuseppe, Mattioli, Anna Vittoria, Maueroder, Christian, Mazzoni, Alessio, McCluskey, James, McGrath, Mairi, McGuire, Helen M., McInnes, Iain B., Mei, Henrik E., Melchers, Fritz, Melzer, Susanne, Mielenz, Dirk, Miller, Stephen D., Mills, Kingston H. G., Minderman, Hans, Mjosberg, Jenny, Moore, Jonni, Moran, Barry, Moretta, Lorenzo, Mosmann, Tim R., Mueller, Susann, Multhoff, Gabriele, Munoz, Luis Enrique, Munz, Christian, Nakayama, Toshinori, Nasi, Milena, Neumann, Katrin, Ng, Lai Guan, Niedobitek, Antonia, Nourshargh, Sussan, Nunez, Gabriel, O'Connor, Jose-Enrique, Ochel, Aaron, Oja, Anna, Ordonez, Diana, Orfao, Alberto, Orlowski-Oliver, Eva, Ouyang, Wenjun, Oxenius, Annette, Palankar, Raghavendra, Panse, Isabel, Pattanapanyasat, Kovit, Paulsen, Malte, Pavlinic, Dinko, Penter, Livius, Peterson, Paert, Peth, Christian, Petriz, Jordi, Piancone, Federica, Pickl, Winfried F., Piconese, Silvia, Pinti, Marcello, Pockley, A. Graham, Podolska, Malgorzata Justyna, Poon, Zhiyong, Pracht, Katharina, Prinz, Immo, Pucillo, Carlo E. M., Quataert, Sally A., Quatrini, Linda, Quinn, Kylie M., Radbruch, Helena, Radstake, Tim R. D. J., Rahmig, Susann, Rahn, Hans-Peter, Rajwa, Bartek, Ravichandran, Gevitha, Raz, Yotam, Rebhahn, Jonathan A., Recktenwald, Diether, Reimer, Dorothea, Reis e Sousa, Caetano, Remmerswaal, Ester B. M., Richter, Lisa, Rico, Laura G., Riddell, Andy, Rieger, Aja M., Robinson, J. Paul, Romagnani, Chiara, Rubartelli, Anna, Ruland, Juergen, Saalmueller, Armin, Saeys, Yvan, Saito, Takashi, Sakaguchi, Shimon, Sala-de-Oyanguren, Francisco, Samstag, Yvonne, Sanderson, Sharon, Sandrock, Inga, Santoni, Angela, Sanz, Ramon Bellmas, Saresella, Marina, Sautes-Fridman, Catherine, Sawitzki, Birgit, Schadt, Linda, Scheffold, Alexander, Scherer, Hans U., Schiemann, Matthias, Schildberg, Frank A., Schimisky, Esther, Schlitzer, Andreas, Schlosser, Josephine, Schmid, Stephan, Schmitt, Steffen, Schober, Kilian, Schraivogel, Daniel, Schuh, Wolfgang, Schueler, Thomas, Schulte, Reiner, Schulz, Axel Ronald, Schulz, Sebastian R., Scotta, Cristiano, Scott-Algara, Daniel, Sester, David P., Shankey, T. Vincent, Silva-Santos, Bruno, Simon, Anna Katharina, Sitnik, Katarzyna M., Sozzani, Silvano, Speiser, Daniel E., Spidlen, Josef, Stahlberg, Anders, Stall, Alan M., Stanley, Natalie, Stark, Regina, Stehle, Christina, Steinmetz, Tobit, Stockinger, Hannes, Takahama, Yousuke, Takeda, Kiyoshi, Tan, Leonard, Tarnok, Attila, Tiegs, Gisa, Toldi, Gergely, Tornack, Julia, Traggiai, Elisabetta, Trebak, Mohamed, Tree, Timothy I. M., Trotter, Joe, Trowsdale, John, Tsoumakidou, Maria, Ulrich, Henning, Urbanczyk, Sophia, van de Veen, Willem, van den Broek, Maries, van der Pol, Edwin, Van Gassen, Sofie, Van Isterdael, Gert, van Lier, Rene A. W., Veldhoen, Marc, Vento-Asturias, Salvador, Vieira, Paulo, Voehringer, David, Volk, Hans-Dieter, von Borstel, Anouk, von Volkmann, Konrad, Waisman, Ari, Walker, Rachael V., Wallace, Paul K., Wang, Sa A., Wang, Xin M., Ward, Michael D., Ward-Hartstonge, Kirsten A., Warnatz, Klaus, Warnes, Gary, Warth, Sarah, Waskow, Claudia, Watson, James V., Watzl, Carsten, Wegener, Leonie, Weisenburger, Thomas, Wiedemann, Annika, Wienands, Juergen, Wilharm, Anneke, Wilkinson, Robert John, Willimsky, Gerald, Wing, James B., Winkelmann, Rieke, Winkler, Thomas H., Wirz, Oliver F., Wong, Alicia, Wurst, Peter, Yang, Jennie H. M., Yang, Juhao, Yazdanbakhsh, Maria, Yu, Liping, Yue, Alice, Zhang, Hanlin, Zhao, Yi, Ziegler, Susanne Maria, Zielinski, Christina, Zimmermann, Jakob, and Zychlinsky, Arturo
Abstract: These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
Published: 2019

20. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Author: Cossarizza, Andrea, Chang, Hyun-Dong, Radbruch, Andreas, Acs, Andreas, Adam, Dieter, Adam-Klages, Sabine, Agace, William W., Aghaeepour, Nima, Akdis, Muebeccel, Allez, Matthieu, Almeida, Larissa Nogueira, Alvisi, Giorgia, Anderson, Graham, Andrae, Immanuel, Annunziato, Francesco, Anselmo, Achille, Bacher, Petra, Baldari, Cosima T., Bari, Sudipto, Barnaba, Vincenzo, Barros-Martins, Joana, Battistini, Luca, Bauer, Wolfgang, Baumgart, Sabine, Baumgarth, Nicole, Baumjohann, Dirk, Baying, Bianka, Bebawy, Mary, Becher, Burkhard, Beisker, Wolfgang, Benes, Vladimir, Beyaert, Rudi, Blanco, Alfonso, Boardman, Dominic A., Bogdan, Christian, Borger, Jessica G., Borsellino, Giovanna, Boulais, Philip E., Bradford, Jolene A., Brenner, Dirk, Brinkman, Ryan R., Brooks, Anna E. S., Busch, Dirk H., Buescher, Martin, Bushnell, Timothy P., Calzetti, Federica, Cameron, Garth, Cammarata, Ilenia, Cao, Xuetao, Cardell, Susanna L., Casola, Stefano, Cassatella, Marco A., Cavani, Andrea, Celada, Antonio, Chatenoud, Lucienne, Chattopadhyay, Pratip K., Chow, Sue, Christakou, Eleni, Cicin-Sain, Luka, Clerici, Mario, Colombo, Federico S., Cook, Laura, Cooke, Anne, Cooper, Andrea M., Corbett, Alexandra J., Cosma, Antonio, Cosmi, Lorenzo, Coulie, Pierre G., Cumano, Ana, Cvetkovic, Ljiljana, Dang, Van Duc, Dang-Heine, Chantip, Davey, Martin S., Davies, Derek, De Biasi, Sara, Del Zotto, Genny, Dela Cruz, Gelo Victoriano, Delacher, Michael, Della Bella, Silvia, Dellabona, Paolo, Deniz, Guennur, Dessing, Mark, Di Santo, James P., Diefenbach, Andreas, Dieli, Francesco, Dolf, Andreas, Doerner, Thomas, Dress, Regine J., Dudziak, Diana, Dustin, Michael, Dutertre, Charles-Antoine, Ebner, Friederike, Eckle, Sidonia B. G., Edinger, Matthias, Eede, Pascale, Ehrhardt, Goetz R. A., Eich, Marcus, Engel, Pablo, Engelhardt, Britta, Erdei, Anna, Esser, Charlotte, Everts, Bart, Evrard, Maximilien, Falk, Christine S., Fehniger, Todd A., Felipo-Benavent, Mar, Ferry, Helen, Feuerer, Markus, Filby, Andrew, Filkor, Kata, Fillatreau, Simon, Follo, Marie, Foerster, Irmgard, Foster, John, Foulds, Gemma A., Frehse, Britta, Frenette, Paul S., Frischbutter, Stefan, Fritzsche, Wolfgang, Galbraith, David W., Gangaev, Anastasia, Garbi, Natalio, Gaudilliere, Brice, Gazzinelli, Ricardo T., Geginat, Jens, Gerner, Wilhelm, Gherardin, Nicholas A., Ghoreschi, Kamran, Gibellini, Lara, Ginhoux, Florent, Goda, Keisuke, Godfrey, Dale I., Goettlinger, Christoph, Gonzalez-Navajas, Jose M., Goodyear, Carl S., Gori, Andrea, Grogan, Jane L., Grummitt, Daryl, Gruetzkau, Andreas, Haftmann, Claudia, Hahn, Jonas, Hammad, Hamida, Haemmerling, Guenter, Hansmann, Leo, Hansson, Goran, Harpur, Christopher M., Hartmann, Susanne, Hauser, Andrea, Hauser, Anja E., Haviland, David L., Hedley, David, Hernandez, Daniela C., Herrera, Guadalupe, Herrmann, Martin, Hess, Christoph, Hoefer, Thomas, Hoffmann, Petra, Hogquist, Kristin, Holland, Tristan, Hollt, Thomas, Holmdahl, Rikard, Hombrink, Pleun, Houston, Jessica P., Hoyer, Bimba F., Huang, Bo, Huang, Fang-Ping, Huber, Johanna E., Huehn, Jochen, Hundemer, Michael, Hunter, Christopher A., Hwang, William Y. K., Iannone, Anna, Ingelfinger, Florian, Ivison, Sabine M., Jaeck, Hans-Martin, Jani, Peter K., Javega, Beatriz, Jonjic, Stipan, Kaiser, Toralf, Kalina, Tomas, Kamradt, Thomas, Kaufmann, Stefan H. E., Keller, Baerbel, Ketelaars, Steven L. C., Khalilnezhad, Ahad, Khan, Srijit, Kisielow, Jan, Klenerman, Paul, Knopf, Jasmin, Koay, Hui-Fern, Kobow, Katja, Kolls, Jay K., Kong, Wan Ting, Kopf, Manfred, Korn, Thomas, Kriegsmann, Katharina, Kristyanto, Hendy, Kroneis, Thomas, Krueger, Andreas, Kuehne, Jenny, Kukat, Christian, Kunkel, Desiree, Kunze-Schumacher, Heike, Kurosaki, Tomohiro, Kurts, Christian, Kvistborg, Pia, Kwok, Immanuel, Landry, Jonathan, Lantz, Olivier, Lanuti, Paola, LaRosa, Francesca, Lehuen, Agnes, LeibundGut-Landmann, Salome, Leipold, Michael D., Leung, Leslie Y. T., Levings, Megan K., Lino, Andreia C., Liotta, Francesco, Litwin, Virginia, Liu, Yanling, Ljunggren, Hans-Gustaf, Lohoff, Michael, Lombardi, Giovanna, Lopez, Lilly, Lopez-Botet, Miguel, Lovett-Racke, Amy E., Lubberts, Erik, Luche, Herve, Ludewig, Burkhard, Lugli, Enrico, Lunemann, Sebastian, Maecker, Holden T., Maggi, Laura, Maguire, Orla, Mair, Florian, Mair, Kerstin H., Mantovani, Alberto, Manz, Rudolf A., Marshall, Aaron J., Martinez-Romero, Alicia, Martrus, Gloria, Marventano, Ivana, Maslinski, Wlodzimierz, Matarese, Giuseppe, Mattioli, Anna Vittoria, Maueroder, Christian, Mazzoni, Alessio, McCluskey, James, McGrath, Mairi, McGuire, Helen M., McInnes, Iain B., Mei, Henrik E., Melchers, Fritz, Melzer, Susanne, Mielenz, Dirk, Miller, Stephen D., Mills, Kingston H. G., Minderman, Hans, Mjosberg, Jenny, Moore, Jonni, Moran, Barry, Moretta, Lorenzo, Mosmann, Tim R., Mueller, Susann, Multhoff, Gabriele, Munoz, Luis Enrique, Munz, Christian, Nakayama, Toshinori, Nasi, Milena, Neumann, Katrin, Ng, Lai Guan, Niedobitek, Antonia, Nourshargh, Sussan, Nunez, Gabriel, O'Connor, Jose-Enrique, Ochel, Aaron, Oja, Anna, Ordonez, Diana, Orfao, Alberto, Orlowski-Oliver, Eva, Ouyang, Wenjun, Oxenius, Annette, Palankar, Raghavendra, Panse, Isabel, Pattanapanyasat, Kovit, Paulsen, Malte, Pavlinic, Dinko, Penter, Livius, Peterson, Paert, Peth, Christian, Petriz, Jordi, Piancone, Federica, Pickl, Winfried F., Piconese, Silvia, Pinti, Marcello, Pockley, A. Graham, Podolska, Malgorzata Justyna, Poon, Zhiyong, Pracht, Katharina, Prinz, Immo, Pucillo, Carlo E. M., Quataert, Sally A., Quatrini, Linda, Quinn, Kylie M., Radbruch, Helena, Radstake, Tim R. D. J., Rahmig, Susann, Rahn, Hans-Peter, Rajwa, Bartek, Ravichandran, Gevitha, Raz, Yotam, Rebhahn, Jonathan A., Recktenwald, Diether, Reimer, Dorothea, Reis e Sousa, Caetano, Remmerswaal, Ester B. M., Richter, Lisa, Rico, Laura G., Riddell, Andy, Rieger, Aja M., Robinson, J. Paul, Romagnani, Chiara, Rubartelli, Anna, Ruland, Juergen, Saalmueller, Armin, Saeys, Yvan, Saito, Takashi, Sakaguchi, Shimon, Sala-de-Oyanguren, Francisco, Samstag, Yvonne, Sanderson, Sharon, Sandrock, Inga, Santoni, Angela, Sanz, Ramon Bellmas, Saresella, Marina, Sautes-Fridman, Catherine, Sawitzki, Birgit, Schadt, Linda, Scheffold, Alexander, Scherer, Hans U., Schiemann, Matthias, Schildberg, Frank A., Schimisky, Esther, Schlitzer, Andreas, Schlosser, Josephine, Schmid, Stephan, Schmitt, Steffen, Schober, Kilian, Schraivogel, Daniel, Schuh, Wolfgang, Schueler, Thomas, Schulte, Reiner, Schulz, Axel Ronald, Schulz, Sebastian R., Scotta, Cristiano, Scott-Algara, Daniel, Sester, David P., Shankey, T. Vincent, Silva-Santos, Bruno, Simon, Anna Katharina, Sitnik, Katarzyna M., Sozzani, Silvano, Speiser, Daniel E., Spidlen, Josef, Stahlberg, Anders, Stall, Alan M., Stanley, Natalie, Stark, Regina, Stehle, Christina, Steinmetz, Tobit, Stockinger, Hannes, Takahama, Yousuke, Takeda, Kiyoshi, Tan, Leonard, Tarnok, Attila, Tiegs, Gisa, Toldi, Gergely, Tornack, Julia, Traggiai, Elisabetta, Trebak, Mohamed, Tree, Timothy I. M., Trotter, Joe, Trowsdale, John, Tsoumakidou, Maria, Ulrich, Henning, Urbanczyk, Sophia, van de Veen, Willem, van den Broek, Maries, van der Pol, Edwin, Van Gassen, Sofie, Van Isterdael, Gert, van Lier, Rene A. W., Veldhoen, Marc, Vento-Asturias, Salvador, Vieira, Paulo, Voehringer, David, Volk, Hans-Dieter, von Borstel, Anouk, von Volkmann, Konrad, Waisman, Ari, Walker, Rachael V., Wallace, Paul K., Wang, Sa A., Wang, Xin M., Ward, Michael D., Ward-Hartstonge, Kirsten A., Warnatz, Klaus, Warnes, Gary, Warth, Sarah, Waskow, Claudia, Watson, James V., Watzl, Carsten, Wegener, Leonie, Weisenburger, Thomas, Wiedemann, Annika, Wienands, Juergen, Wilharm, Anneke, Wilkinson, Robert John, Willimsky, Gerald, Wing, James B., Winkelmann, Rieke, Winkler, Thomas H., Wirz, Oliver F., Wong, Alicia, Wurst, Peter, Yang, Jennie H. M., Yang, Juhao, Yazdanbakhsh, Maria, Yu, Liping, Yue, Alice, Zhang, Hanlin, Zhao, Yi, Ziegler, Susanne Maria, Zielinski, Christina, Zimmermann, Jakob, Zychlinsky, Arturo, Cossarizza, Andrea, Chang, Hyun-Dong, Radbruch, Andreas, Acs, Andreas, Adam, Dieter, Adam-Klages, Sabine, Agace, William W., Aghaeepour, Nima, Akdis, Muebeccel, Allez, Matthieu, Almeida, Larissa Nogueira, Alvisi, Giorgia, Anderson, Graham, Andrae, Immanuel, Annunziato, Francesco, Anselmo, Achille, Bacher, Petra, Baldari, Cosima T., Bari, Sudipto, Barnaba, Vincenzo, Barros-Martins, Joana, Battistini, Luca, Bauer, Wolfgang, Baumgart, Sabine, Baumgarth, Nicole, Baumjohann, Dirk, Baying, Bianka, Bebawy, Mary, Becher, Burkhard, Beisker, Wolfgang, Benes, Vladimir, Beyaert, Rudi, Blanco, Alfonso, Boardman, Dominic A., Bogdan, Christian, Borger, Jessica G., Borsellino, Giovanna, Boulais, Philip E., Bradford, Jolene A., Brenner, Dirk, Brinkman, Ryan R., Brooks, Anna E. S., Busch, Dirk H., Buescher, Martin, Bushnell, Timothy P., Calzetti, Federica, Cameron, Garth, Cammarata, Ilenia, Cao, Xuetao, Cardell, Susanna L., Casola, Stefano, Cassatella, Marco A., Cavani, Andrea, Celada, Antonio, Chatenoud, Lucienne, Chattopadhyay, Pratip K., Chow, Sue, Christakou, Eleni, Cicin-Sain, Luka, Clerici, Mario, Colombo, Federico S., Cook, Laura, Cooke, Anne, Cooper, Andrea M., Corbett, Alexandra J., Cosma, Antonio, Cosmi, Lorenzo, Coulie, Pierre G., Cumano, Ana, Cvetkovic, Ljiljana, Dang, Van Duc, Dang-Heine, Chantip, Davey, Martin S., Davies, Derek, De Biasi, Sara, Del Zotto, Genny, Dela Cruz, Gelo Victoriano, Delacher, Michael, Della Bella, Silvia, Dellabona, Paolo, Deniz, Guennur, Dessing, Mark, Di Santo, James P., Diefenbach, Andreas, Dieli, Francesco, Dolf, Andreas, Doerner, Thomas, Dress, Regine J., Dudziak, Diana, Dustin, Michael, Dutertre, Charles-Antoine, Ebner, Friederike, Eckle, Sidonia B. G., Edinger, Matthias, Eede, Pascale, Ehrhardt, Goetz R. A., Eich, Marcus, Engel, Pablo, Engelhardt, Britta, Erdei, Anna, Esser, Charlotte, Everts, Bart, Evrard, Maximilien, Falk, Christine S., Fehniger, Todd A., Felipo-Benavent, Mar, Ferry, Helen, Feuerer, Markus, Filby, Andrew, Filkor, Kata, Fillatreau, Simon, Follo, Marie, Foerster, Irmgard, Foster, John, Foulds, Gemma A., Frehse, Britta, Frenette, Paul S., Frischbutter, Stefan, Fritzsche, Wolfgang, Galbraith, David W., Gangaev, Anastasia, Garbi, Natalio, Gaudilliere, Brice, Gazzinelli, Ricardo T., Geginat, Jens, Gerner, Wilhelm, Gherardin, Nicholas A., Ghoreschi, Kamran, Gibellini, Lara, Ginhoux, Florent, Goda, Keisuke, Godfrey, Dale I., Goettlinger, Christoph, Gonzalez-Navajas, Jose M., Goodyear, Carl S., Gori, Andrea, Grogan, Jane L., Grummitt, Daryl, Gruetzkau, Andreas, Haftmann, Claudia, Hahn, Jonas, Hammad, Hamida, Haemmerling, Guenter, Hansmann, Leo, Hansson, Goran, Harpur, Christopher M., Hartmann, Susanne, Hauser, Andrea, Hauser, Anja E., Haviland, David L., Hedley, David, Hernandez, Daniela C., Herrera, Guadalupe, Herrmann, Martin, Hess, Christoph, Hoefer, Thomas, Hoffmann, Petra, Hogquist, Kristin, Holland, Tristan, Hollt, Thomas, Holmdahl, Rikard, Hombrink, Pleun, Houston, Jessica P., Hoyer, Bimba F., Huang, Bo, Huang, Fang-Ping, Huber, Johanna E., Huehn, Jochen, Hundemer, Michael, Hunter, Christopher A., Hwang, William Y. K., Iannone, Anna, Ingelfinger, Florian, Ivison, Sabine M., Jaeck, Hans-Martin, Jani, Peter K., Javega, Beatriz, Jonjic, Stipan, Kaiser, Toralf, Kalina, Tomas, Kamradt, Thomas, Kaufmann, Stefan H. E., Keller, Baerbel, Ketelaars, Steven L. C., Khalilnezhad, Ahad, Khan, Srijit, Kisielow, Jan, Klenerman, Paul, Knopf, Jasmin, Koay, Hui-Fern, Kobow, Katja, Kolls, Jay K., Kong, Wan Ting, Kopf, Manfred, Korn, Thomas, Kriegsmann, Katharina, Kristyanto, Hendy, Kroneis, Thomas, Krueger, Andreas, Kuehne, Jenny, Kukat, Christian, Kunkel, Desiree, Kunze-Schumacher, Heike, Kurosaki, Tomohiro, Kurts, Christian, Kvistborg, Pia, Kwok, Immanuel, Landry, Jonathan, Lantz, Olivier, Lanuti, Paola, LaRosa, Francesca, Lehuen, Agnes, LeibundGut-Landmann, Salome, Leipold, Michael D., Leung, Leslie Y. T., Levings, Megan K., Lino, Andreia C., Liotta, Francesco, Litwin, Virginia, Liu, Yanling, Ljunggren, Hans-Gustaf, Lohoff, Michael, Lombardi, Giovanna, Lopez, Lilly, Lopez-Botet, Miguel, Lovett-Racke, Amy E., Lubberts, Erik, Luche, Herve, Ludewig, Burkhard, Lugli, Enrico, Lunemann, Sebastian, Maecker, Holden T., Maggi, Laura, Maguire, Orla, Mair, Florian, Mair, Kerstin H., Mantovani, Alberto, Manz, Rudolf A., Marshall, Aaron J., Martinez-Romero, Alicia, Martrus, Gloria, Marventano, Ivana, Maslinski, Wlodzimierz, Matarese, Giuseppe, Mattioli, Anna Vittoria, Maueroder, Christian, Mazzoni, Alessio, McCluskey, James, McGrath, Mairi, McGuire, Helen M., McInnes, Iain B., Mei, Henrik E., Melchers, Fritz, Melzer, Susanne, Mielenz, Dirk, Miller, Stephen D., Mills, Kingston H. G., Minderman, Hans, Mjosberg, Jenny, Moore, Jonni, Moran, Barry, Moretta, Lorenzo, Mosmann, Tim R., Mueller, Susann, Multhoff, Gabriele, Munoz, Luis Enrique, Munz, Christian, Nakayama, Toshinori, Nasi, Milena, Neumann, Katrin, Ng, Lai Guan, Niedobitek, Antonia, Nourshargh, Sussan, Nunez, Gabriel, O'Connor, Jose-Enrique, Ochel, Aaron, Oja, Anna, Ordonez, Diana, Orfao, Alberto, Orlowski-Oliver, Eva, Ouyang, Wenjun, Oxenius, Annette, Palankar, Raghavendra, Panse, Isabel, Pattanapanyasat, Kovit, Paulsen, Malte, Pavlinic, Dinko, Penter, Livius, Peterson, Paert, Peth, Christian, Petriz, Jordi, Piancone, Federica, Pickl, Winfried F., Piconese, Silvia, Pinti, Marcello, Pockley, A. Graham, Podolska, Malgorzata Justyna, Poon, Zhiyong, Pracht, Katharina, Prinz, Immo, Pucillo, Carlo E. M., Quataert, Sally A., Quatrini, Linda, Quinn, Kylie M., Radbruch, Helena, Radstake, Tim R. D. J., Rahmig, Susann, Rahn, Hans-Peter, Rajwa, Bartek, Ravichandran, Gevitha, Raz, Yotam, Rebhahn, Jonathan A., Recktenwald, Diether, Reimer, Dorothea, Reis e Sousa, Caetano, Remmerswaal, Ester B. M., Richter, Lisa, Rico, Laura G., Riddell, Andy, Rieger, Aja M., Robinson, J. Paul, Romagnani, Chiara, Rubartelli, Anna, Ruland, Juergen, Saalmueller, Armin, Saeys, Yvan, Saito, Takashi, Sakaguchi, Shimon, Sala-de-Oyanguren, Francisco, Samstag, Yvonne, Sanderson, Sharon, Sandrock, Inga, Santoni, Angela, Sanz, Ramon Bellmas, Saresella, Marina, Sautes-Fridman, Catherine, Sawitzki, Birgit, Schadt, Linda, Scheffold, Alexander, Scherer, Hans U., Schiemann, Matthias, Schildberg, Frank A., Schimisky, Esther, Schlitzer, Andreas, Schlosser, Josephine, Schmid, Stephan, Schmitt, Steffen, Schober, Kilian, Schraivogel, Daniel, Schuh, Wolfgang, Schueler, Thomas, Schulte, Reiner, Schulz, Axel Ronald, Schulz, Sebastian R., Scotta, Cristiano, Scott-Algara, Daniel, Sester, David P., Shankey, T. Vincent, Silva-Santos, Bruno, Simon, Anna Katharina, Sitnik, Katarzyna M., Sozzani, Silvano, Speiser, Daniel E., Spidlen, Josef, Stahlberg, Anders, Stall, Alan M., Stanley, Natalie, Stark, Regina, Stehle, Christina, Steinmetz, Tobit, Stockinger, Hannes, Takahama, Yousuke, Takeda, Kiyoshi, Tan, Leonard, Tarnok, Attila, Tiegs, Gisa, Toldi, Gergely, Tornack, Julia, Traggiai, Elisabetta, Trebak, Mohamed, Tree, Timothy I. M., Trotter, Joe, Trowsdale, John, Tsoumakidou, Maria, Ulrich, Henning, Urbanczyk, Sophia, van de Veen, Willem, van den Broek, Maries, van der Pol, Edwin, Van Gassen, Sofie, Van Isterdael, Gert, van Lier, Rene A. W., Veldhoen, Marc, Vento-Asturias, Salvador, Vieira, Paulo, Voehringer, David, Volk, Hans-Dieter, von Borstel, Anouk, von Volkmann, Konrad, Waisman, Ari, Walker, Rachael V., Wallace, Paul K., Wang, Sa A., Wang, Xin M., Ward, Michael D., Ward-Hartstonge, Kirsten A., Warnatz, Klaus, Warnes, Gary, Warth, Sarah, Waskow, Claudia, Watson, James V., Watzl, Carsten, Wegener, Leonie, Weisenburger, Thomas, Wiedemann, Annika, Wienands, Juergen, Wilharm, Anneke, Wilkinson, Robert John, Willimsky, Gerald, Wing, James B., Winkelmann, Rieke, Winkler, Thomas H., Wirz, Oliver F., Wong, Alicia, Wurst, Peter, Yang, Jennie H. M., Yang, Juhao, Yazdanbakhsh, Maria, Yu, Liping, Yue, Alice, Zhang, Hanlin, Zhao, Yi, Ziegler, Susanne Maria, Zielinski, Christina, Zimmermann, Jakob, and Zychlinsky, Arturo
Abstract: These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
Published: 2019

21. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Author: Cossarizza, Andrea, Chang, Hyun-Dong, Radbruch, Andreas, Acs, Andreas, Adam, Dieter, Adam-Klages, Sabine, Agace, William W, Aghaeepour, Nima, Akdis, Mübeccel, Allez, Matthieu, Almeida, Larissa Nogueira, Alvisi, Giorgia, Anderson, Graham, Andrä, Immanuel, Annunziato, Francesco, Anselmo, Achille, Bacher, Petra, Baldari, Cosima T, Bari, Sudipto, Barnaba, Vincenzo, Barros-Martins, Joana, Battistini, Luca, Bauer, Wolfgang, Baumgart, Sabine, Baumgarth, Nicole, Baumjohann, Dirk, Baying, Bianka, Bebawy, Mary, Becher, Burkhard, Beisker, Wolfgang, Benes, Vladimir, Beyaert, Rudi, Blanco, Alfonso, Boardman, Dominic A, Bogdan, Christian, Borger, Jessica G, Borsellino, Giovanna, Boulais, Philip E, Bradford, Jolene A, Brenner, Dirk, Brinkman, Ryan R, Brooks, Anna E S, Busch, Dirk H, Büscher, Martin, Bushnell, Timothy P, Calzetti, Federica, Cameron, Garth, Cammarata, Ilenia, Cao, Xuetao, Cardell, Susanna L, Casola, Stefano, Cassatella, Marco A, Cavani, Andrea, Celada, Antonio, Chatenoud, Lucienne, Chattopadhyay, Pratip K, Chow, Sue, Christakou, Eleni, Čičin-Šain, Luka, Clerici, Mario, Colombo, Federico S, Cook, Laura, Cooke, Anne, Cooper, Andrea M, Corbett, Alexandra J, Cosma, Antonio, Cosmi, Lorenzo, Coulie, Pierre G, Cumano, Ana, Cvetkovic, Ljiljana, Dang, Van Duc, Dang-Heine, Chantip, Davey, Martin S, Davies, Derek, De Biasi, Sara, Del Zotto, Genny, Dela Cruz, Gelo Victoriano, Delacher, Michael, Della Bella, Silvia, Dellabona, Paolo, Deniz, Günnur, Dessing, Mark, Di Santo, James P, Diefenbach, Andreas, Dieli, Francesco, Dolf, Andreas, Dörner, Thomas, Dress, Regine J, Dudziak, Diana, Dustin, Michael, Dutertre, Charles-Antoine, Ebner, Friederike, Eckle, Sidonia B G, Edinger, Matthias, Eede, Pascale, Ehrhardt, Götz R A, Eich, Marcus, Engel, Pablo, Engelhardt, Britta, Erdei, Anna, Esser, Charlotte, Everts, Bart, Evrard, Maximilien, Falk, Christine S, Fehniger, Todd A, Felipo-Benavent, Mar, Ferry, Helen, Feuerer, Markus, Filby, Andrew, Filkor, Kata, Fillatreau, Simon, Follo, Marie, Förster, Irmgard, Foster, John, Foulds, Gemma A, Frehse, Britta, Frenette, Paul S, Frischbutter, Stefan, Fritzsche, Wolfgang, Galbraith, David W, Gangaev, Anastasia, Garbi, Natalio, Gaudilliere, Brice, Gazzinelli, Ricardo T, Geginat, Jens, Gerner, Wilhelm, Gherardin, Nicholas A, Ghoreschi, Kamran, Gibellini, Lara, Ginhoux, Florent, Goda, Keisuke, Godfrey, Dale, Goettlinger, Christoph, González-Navajas, Jose M, Goodyear, Carl S, Gori, Andrea, Grogan, Jane L, Grummitt, Daryl, Grützkau, Andreas, Haftmann, Claudia, Hahn, Jonas, Hammad, Hamida, Hämmerling, Günter, Hansmann, Leo, Hansson, Goran, Harpur, Christopher M, Hartmann, Susanne, Hauser, Andrea, Hauser, Anja E, Haviland, David L, Hedley, David, Hernández, Daniela C, Herrera, Guadalupe, Herrmann, Martin, Hess, Christoph, Höfer, Thomas, Hoffmann, Petra, Hogquist, Kristin, Holland, Tristan, Höllt, Thomas, Holmdahl, Rikard, Hombrink, Pleun, Houston, Jessica P, Hoyer, Bimba F, Huang, Bo, Huang, Fang-Ping, Huber, Johanna E, Huehn, Jochen, Hundemer, Michael, Hunter, Christopher A, Hwang, William Y K, Iannone, Anna, Ingelfinger, Florian, Ivison, Sabine M, Jäck, Hans-Martin, Jani, Peter K, Jávega, Beatriz, Jonjic, Stipan, Kaiser, Toralf, Kalina, Tomas, Kamradt, Thomas, Kaufmann, Stefan H E, Keller, Baerbel, Ketelaars, Steven L C, Khalilnezhad, Ahad, Khan, Srijit, Kisielow, Jan, Klenerman, Paul, Knopf, Jasmin, Koay, Hui-Fern, Kobow, Katja, Kolls, Jay K, Kong, Wan Ting, Kopf, Manfred, Korn, Thomas, Kriegsmann, Katharina, Kristyanto, Hendy, Kroneis, Thomas, Krueger, Andreas, Kühne, Jenny, Kukat, Christian, Kunkel, Désirée, Kunze-Schumacher, Heike, Kurosaki, Tomohiro, Kurts, Christian, Kvistborg, Pia, Kwok, Immanuel, Landry, Jonathan, Lantz, Olivier, Lanuti, Paola, LaRosa, Francesca, Lehuen, Agnès, LeibundGut-Landmann, Salomé, Leipold, Michael D, Leung, Leslie Y T, Levings, Megan K, Lino, Andreia C, Liotta, Francesco, Litwin, Virginia, Liu, Yanling, Ljunggren, Hans-Gustaf, Lohoff, Michael, Lombardi, Giovanna, Lopez, Lilly, López-Botet, Miguel, Lovett-Racke, Amy E, Lubberts, Erik, Luche, Herve, Ludewig, Burkhard, Lugli, Enrico, Lunemann, Sebastian, Maecker, Holden T, Maggi, Laura, Maguire, Orla, Mair, Florian, Mair, Kerstin H, Mantovani, Alberto, Manz, Rudolf A, Marshall, Aaron J, Martínez-Romero, Alicia, Martrus, Glòria, Marventano, Ivana, Maslinski, Wlodzimierz, Matarese, Giuseppe, Mattioli, Anna Vittoria, Maueröder, Christian, Mazzoni, Alessio, McCluskey, James, McGrath, Mairi, McGuire, Helen M, McInnes, Iain B, Mei, Henrik E, Melchers, Fritz, Melzer, Susanne, Mielenz, Dirk, Miller, Stephen D, Mills, Kingston H G, Minderman, Hans, Mjösberg, Jenny, Moore, Jonni, Moran, Barry, Moretta, Lorenzo, Mosmann, Tim R, Müller, Susann, Multhoff, Gabriele, Muñoz, Luis Enrique, Münz, Christian, Nakayama, Toshinori, Nasi, Milena, Neumann, Katrin, Ng, Lai Guan, Niedobitek, Antonia, Nourshargh, Sussan, Núñez, Gabriel, O'Connor, José-Enrique, Ochel, Aaron, Oja, Anna, Ordonez, Diana, Orfao, Alberto, Orlowski-Oliver, Eva, Ouyang, Wenjun, Oxenius, Annette, Palankar, Raghavendra, Panse, Isabel, Pattanapanyasat, Kovit, Paulsen, Malte, Pavlinic, Dinko, Penter, Livius, Peterson, Pärt, Peth, Christian, Petriz, Jordi, Piancone, Federica, Pickl, Winfried F, Piconese, Silvia, Pinti, Marcello, Pockley, A Graham, Podolska, Malgorzata Justyna, Poon, Zhiyong, Pracht, Katharina, Prinz, Immo, Pucillo, Carlo E M, Quataert, Sally A, Quatrini, Linda, Quinn, Kylie M, Radbruch, Helena, Radstake, Tim R D J, Rahmig, Susann, Rahn, Hans-Peter, Rajwa, Bartek, Ravichandran, Gevitha, Raz, Yotam, Rebhahn, Jonathan A, Recktenwald, Diether, Reimer, Dorothea, Reis E Sousa, Caetano, Remmerswaal, Ester B M, Richter, Lisa, Rico, Laura G, Riddell, Andy, Rieger, Aja M, Robinson, J Paul, Romagnani, Chiara, Rubartelli, Anna, Ruland, Jürgen, Saalmüller, Armin, Saeys, Yvan, Saito, Takashi, Sakaguchi, Shimon, Sala-de-Oyanguren, Francisco, Samstag, Yvonne, Sanderson, Sharon, Sandrock, Inga, Santoni, Angela, Sanz, Ramon Bellmàs, Saresella, Marina, Sautes-Fridman, Catherine, Sawitzki, Birgit, Schadt, Linda, Scheffold, Alexander, Scherer, Hans U, Schiemann, Matthias, Schildberg, Frank A, Schimisky, Esther, Schlitzer, Andreas, Schlosser, Josephine, Schmid, Stephan, Schmitt, Steffen, Schober, Kilian, Schraivogel, Daniel, Schuh, Wolfgang, Schüler, Thomas, Schulte, Reiner, Schulz, Axel Ronald, Schulz, Sebastian R, Scottá, Cristiano, Scott-Algara, Daniel, Sester, David P, Shankey, T Vincent, Silva-Santos, Bruno, Simon, Anna Katharina, Sitnik, Katarzyna M, Sozzani, Silvano, Speiser, Daniel E, Spidlen, Josef, Stahlberg, Anders, Stall, Alan M, Stanley, Natalie, Stark, Regina, Stehle, Christina, Steinmetz, Tobit, Stockinger, Hannes, Takahama, Yousuke, Takeda, Kiyoshi, Tan, Leonard, Tárnok, Attila, Tiegs, Gisa, Toldi, Gergely, Tornack, Julia, Traggiai, Elisabetta, Trebak, Mohamed, Tree, Timothy I M, Trotter, Joe, Trowsdale, John, Tsoumakidou, Maria, Ulrich, Henning, Urbanczyk, Sophia, van de Veen, Willem, van den Broek, Maries, van der Pol, Edwin, Van Gassen, Sofie, Van Isterdael, Gert, van Lier, René A W, Veldhoen, Marc, Vento-Asturias, Salvador, Vieira, Paulo, Voehringer, David, Volk, Hans-Dieter, von Borstel, Anouk, von Volkmann, Konrad, Waisman, Ari, Walker, Rachael V, Wallace, Paul K, Wang, Sa A, Wang, Xin M, Ward, Michael D, Ward-Hartstonge, Kirsten A, Warnatz, Klaus, Warnes, Gary, Warth, Sarah, Waskow, Claudia, Watson, James V, Watzl, Carsten, Wegener, Leonie, Weisenburger, Thomas, Wiedemann, Annika, Wienands, Jürgen, Wilharm, Anneke, Wilkinson, Robert John, Willimsky, Gerald, Wing, James B, Winkelmann, Rieke, Winkler, Thomas H, Wirz, Oliver F, Wong, Alicia, Wurst, Peter, Yang, Jennie H M, Yang, Juhao, Yazdanbakhsh, Maria, Yu, Liping, Yue, Alice, Zhang, Hanlin, Zhao, Yi, Ziegler, Susanne Maria, Zielinski, Christina, Zimmermann, Jakob, Zychlinsky, Arturo, Cossarizza, Andrea, Chang, Hyun-Dong, Radbruch, Andreas, Acs, Andreas, Adam, Dieter, Adam-Klages, Sabine, Agace, William W, Aghaeepour, Nima, Akdis, Mübeccel, Allez, Matthieu, Almeida, Larissa Nogueira, Alvisi, Giorgia, Anderson, Graham, Andrä, Immanuel, Annunziato, Francesco, Anselmo, Achille, Bacher, Petra, Baldari, Cosima T, Bari, Sudipto, Barnaba, Vincenzo, Barros-Martins, Joana, Battistini, Luca, Bauer, Wolfgang, Baumgart, Sabine, Baumgarth, Nicole, Baumjohann, Dirk, Baying, Bianka, Bebawy, Mary, Becher, Burkhard, Beisker, Wolfgang, Benes, Vladimir, Beyaert, Rudi, Blanco, Alfonso, Boardman, Dominic A, Bogdan, Christian, Borger, Jessica G, Borsellino, Giovanna, Boulais, Philip E, Bradford, Jolene A, Brenner, Dirk, Brinkman, Ryan R, Brooks, Anna E S, Busch, Dirk H, Büscher, Martin, Bushnell, Timothy P, Calzetti, Federica, Cameron, Garth, Cammarata, Ilenia, Cao, Xuetao, Cardell, Susanna L, Casola, Stefano, Cassatella, Marco A, Cavani, Andrea, Celada, Antonio, Chatenoud, Lucienne, Chattopadhyay, Pratip K, Chow, Sue, Christakou, Eleni, Čičin-Šain, Luka, Clerici, Mario, Colombo, Federico S, Cook, Laura, Cooke, Anne, Cooper, Andrea M, Corbett, Alexandra J, Cosma, Antonio, Cosmi, Lorenzo, Coulie, Pierre G, Cumano, Ana, Cvetkovic, Ljiljana, Dang, Van Duc, Dang-Heine, Chantip, Davey, Martin S, Davies, Derek, De Biasi, Sara, Del Zotto, Genny, Dela Cruz, Gelo Victoriano, Delacher, Michael, Della Bella, Silvia, Dellabona, Paolo, Deniz, Günnur, Dessing, Mark, Di Santo, James P, Diefenbach, Andreas, Dieli, Francesco, Dolf, Andreas, Dörner, Thomas, Dress, Regine J, Dudziak, Diana, Dustin, Michael, Dutertre, Charles-Antoine, Ebner, Friederike, Eckle, Sidonia B G, Edinger, Matthias, Eede, Pascale, Ehrhardt, Götz R A, Eich, Marcus, Engel, Pablo, Engelhardt, Britta, Erdei, Anna, Esser, Charlotte, Everts, Bart, Evrard, Maximilien, Falk, Christine S, Fehniger, Todd A, Felipo-Benavent, Mar, Ferry, Helen, Feuerer, Markus, Filby, Andrew, Filkor, Kata, Fillatreau, Simon, Follo, Marie, Förster, Irmgard, Foster, John, Foulds, Gemma A, Frehse, Britta, Frenette, Paul S, Frischbutter, Stefan, Fritzsche, Wolfgang, Galbraith, David W, Gangaev, Anastasia, Garbi, Natalio, Gaudilliere, Brice, Gazzinelli, Ricardo T, Geginat, Jens, Gerner, Wilhelm, Gherardin, Nicholas A, Ghoreschi, Kamran, Gibellini, Lara, Ginhoux, Florent, Goda, Keisuke, Godfrey, Dale, Goettlinger, Christoph, González-Navajas, Jose M, Goodyear, Carl S, Gori, Andrea, Grogan, Jane L, Grummitt, Daryl, Grützkau, Andreas, Haftmann, Claudia, Hahn, Jonas, Hammad, Hamida, Hämmerling, Günter, Hansmann, Leo, Hansson, Goran, Harpur, Christopher M, Hartmann, Susanne, Hauser, Andrea, Hauser, Anja E, Haviland, David L, Hedley, David, Hernández, Daniela C, Herrera, Guadalupe, Herrmann, Martin, Hess, Christoph, Höfer, Thomas, Hoffmann, Petra, Hogquist, Kristin, Holland, Tristan, Höllt, Thomas, Holmdahl, Rikard, Hombrink, Pleun, Houston, Jessica P, Hoyer, Bimba F, Huang, Bo, Huang, Fang-Ping, Huber, Johanna E, Huehn, Jochen, Hundemer, Michael, Hunter, Christopher A, Hwang, William Y K, Iannone, Anna, Ingelfinger, Florian, Ivison, Sabine M, Jäck, Hans-Martin, Jani, Peter K, Jávega, Beatriz, Jonjic, Stipan, Kaiser, Toralf, Kalina, Tomas, Kamradt, Thomas, Kaufmann, Stefan H E, Keller, Baerbel, Ketelaars, Steven L C, Khalilnezhad, Ahad, Khan, Srijit, Kisielow, Jan, Klenerman, Paul, Knopf, Jasmin, Koay, Hui-Fern, Kobow, Katja, Kolls, Jay K, Kong, Wan Ting, Kopf, Manfred, Korn, Thomas, Kriegsmann, Katharina, Kristyanto, Hendy, Kroneis, Thomas, Krueger, Andreas, Kühne, Jenny, Kukat, Christian, Kunkel, Désirée, Kunze-Schumacher, Heike, Kurosaki, Tomohiro, Kurts, Christian, Kvistborg, Pia, Kwok, Immanuel, Landry, Jonathan, Lantz, Olivier, Lanuti, Paola, LaRosa, Francesca, Lehuen, Agnès, LeibundGut-Landmann, Salomé, Leipold, Michael D, Leung, Leslie Y T, Levings, Megan K, Lino, Andreia C, Liotta, Francesco, Litwin, Virginia, Liu, Yanling, Ljunggren, Hans-Gustaf, Lohoff, Michael, Lombardi, Giovanna, Lopez, Lilly, López-Botet, Miguel, Lovett-Racke, Amy E, Lubberts, Erik, Luche, Herve, Ludewig, Burkhard, Lugli, Enrico, Lunemann, Sebastian, Maecker, Holden T, Maggi, Laura, Maguire, Orla, Mair, Florian, Mair, Kerstin H, Mantovani, Alberto, Manz, Rudolf A, Marshall, Aaron J, Martínez-Romero, Alicia, Martrus, Glòria, Marventano, Ivana, Maslinski, Wlodzimierz, Matarese, Giuseppe, Mattioli, Anna Vittoria, Maueröder, Christian, Mazzoni, Alessio, McCluskey, James, McGrath, Mairi, McGuire, Helen M, McInnes, Iain B, Mei, Henrik E, Melchers, Fritz, Melzer, Susanne, Mielenz, Dirk, Miller, Stephen D, Mills, Kingston H G, Minderman, Hans, Mjösberg, Jenny, Moore, Jonni, Moran, Barry, Moretta, Lorenzo, Mosmann, Tim R, Müller, Susann, Multhoff, Gabriele, Muñoz, Luis Enrique, Münz, Christian, Nakayama, Toshinori, Nasi, Milena, Neumann, Katrin, Ng, Lai Guan, Niedobitek, Antonia, Nourshargh, Sussan, Núñez, Gabriel, O'Connor, José-Enrique, Ochel, Aaron, Oja, Anna, Ordonez, Diana, Orfao, Alberto, Orlowski-Oliver, Eva, Ouyang, Wenjun, Oxenius, Annette, Palankar, Raghavendra, Panse, Isabel, Pattanapanyasat, Kovit, Paulsen, Malte, Pavlinic, Dinko, Penter, Livius, Peterson, Pärt, Peth, Christian, Petriz, Jordi, Piancone, Federica, Pickl, Winfried F, Piconese, Silvia, Pinti, Marcello, Pockley, A Graham, Podolska, Malgorzata Justyna, Poon, Zhiyong, Pracht, Katharina, Prinz, Immo, Pucillo, Carlo E M, Quataert, Sally A, Quatrini, Linda, Quinn, Kylie M, Radbruch, Helena, Radstake, Tim R D J, Rahmig, Susann, Rahn, Hans-Peter, Rajwa, Bartek, Ravichandran, Gevitha, Raz, Yotam, Rebhahn, Jonathan A, Recktenwald, Diether, Reimer, Dorothea, Reis E Sousa, Caetano, Remmerswaal, Ester B M, Richter, Lisa, Rico, Laura G, Riddell, Andy, Rieger, Aja M, Robinson, J Paul, Romagnani, Chiara, Rubartelli, Anna, Ruland, Jürgen, Saalmüller, Armin, Saeys, Yvan, Saito, Takashi, Sakaguchi, Shimon, Sala-de-Oyanguren, Francisco, Samstag, Yvonne, Sanderson, Sharon, Sandrock, Inga, Santoni, Angela, Sanz, Ramon Bellmàs, Saresella, Marina, Sautes-Fridman, Catherine, Sawitzki, Birgit, Schadt, Linda, Scheffold, Alexander, Scherer, Hans U, Schiemann, Matthias, Schildberg, Frank A, Schimisky, Esther, Schlitzer, Andreas, Schlosser, Josephine, Schmid, Stephan, Schmitt, Steffen, Schober, Kilian, Schraivogel, Daniel, Schuh, Wolfgang, Schüler, Thomas, Schulte, Reiner, Schulz, Axel Ronald, Schulz, Sebastian R, Scottá, Cristiano, Scott-Algara, Daniel, Sester, David P, Shankey, T Vincent, Silva-Santos, Bruno, Simon, Anna Katharina, Sitnik, Katarzyna M, Sozzani, Silvano, Speiser, Daniel E, Spidlen, Josef, Stahlberg, Anders, Stall, Alan M, Stanley, Natalie, Stark, Regina, Stehle, Christina, Steinmetz, Tobit, Stockinger, Hannes, Takahama, Yousuke, Takeda, Kiyoshi, Tan, Leonard, Tárnok, Attila, Tiegs, Gisa, Toldi, Gergely, Tornack, Julia, Traggiai, Elisabetta, Trebak, Mohamed, Tree, Timothy I M, Trotter, Joe, Trowsdale, John, Tsoumakidou, Maria, Ulrich, Henning, Urbanczyk, Sophia, van de Veen, Willem, van den Broek, Maries, van der Pol, Edwin, Van Gassen, Sofie, Van Isterdael, Gert, van Lier, René A W, Veldhoen, Marc, Vento-Asturias, Salvador, Vieira, Paulo, Voehringer, David, Volk, Hans-Dieter, von Borstel, Anouk, von Volkmann, Konrad, Waisman, Ari, Walker, Rachael V, Wallace, Paul K, Wang, Sa A, Wang, Xin M, Ward, Michael D, Ward-Hartstonge, Kirsten A, Warnatz, Klaus, Warnes, Gary, Warth, Sarah, Waskow, Claudia, Watson, James V, Watzl, Carsten, Wegener, Leonie, Weisenburger, Thomas, Wiedemann, Annika, Wienands, Jürgen, Wilharm, Anneke, Wilkinson, Robert John, Willimsky, Gerald, Wing, James B, Winkelmann, Rieke, Winkler, Thomas H, Wirz, Oliver F, Wong, Alicia, Wurst, Peter, Yang, Jennie H M, Yang, Juhao, Yazdanbakhsh, Maria, Yu, Liping, Yue, Alice, Zhang, Hanlin, Zhao, Yi, Ziegler, Susanne Maria, Zielinski, Christina, Zimmermann, Jakob, and Zychlinsky, Arturo
Abstract: These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
Published: 2019

22. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Author: Cossarizza, Andrea, Chang, Hyun-Dong, Radbruch, Andreas, Acs, Andreas, Adam, Dieter, Adam-Klages, Sabine, Agace, William W., Aghaeepour, Nima, Akdis, Muebeccel, Allez, Matthieu, Almeida, Larissa Nogueira, Alvisi, Giorgia, Anderson, Graham, Andrae, Immanuel, Annunziato, Francesco, Anselmo, Achille, Bacher, Petra, Baldari, Cosima T., Bari, Sudipto, Barnaba, Vincenzo, Barros-Martins, Joana, Battistini, Luca, Bauer, Wolfgang, Baumgart, Sabine, Baumgarth, Nicole, Baumjohann, Dirk, Baying, Bianka, Bebawy, Mary, Becher, Burkhard, Beisker, Wolfgang, Benes, Vladimir, Beyaert, Rudi, Blanco, Alfonso, Boardman, Dominic A., Bogdan, Christian, Borger, Jessica G., Borsellino, Giovanna, Boulais, Philip E., Bradford, Jolene A., Brenner, Dirk, Brinkman, Ryan R., Brooks, Anna E. S., Busch, Dirk H., Buescher, Martin, Bushnell, Timothy P., Calzetti, Federica, Cameron, Garth, Cammarata, Ilenia, Cao, Xuetao, Cardell, Susanna L., Casola, Stefano, Cassatella, Marco A., Cavani, Andrea, Celada, Antonio, Chatenoud, Lucienne, Chattopadhyay, Pratip K., Chow, Sue, Christakou, Eleni, Cicin-Sain, Luka, Clerici, Mario, Colombo, Federico S., Cook, Laura, Cooke, Anne, Cooper, Andrea M., Corbett, Alexandra J., Cosma, Antonio, Cosmi, Lorenzo, Coulie, Pierre G., Cumano, Ana, Cvetkovic, Ljiljana, Dang, Van Duc, Dang-Heine, Chantip, Davey, Martin S., Davies, Derek, De Biasi, Sara, Del Zotto, Genny, Dela Cruz, Gelo Victoriano, Delacher, Michael, Della Bella, Silvia, Dellabona, Paolo, Deniz, Guennur, Dessing, Mark, Di Santo, James P., Diefenbach, Andreas, Dieli, Francesco, Dolf, Andreas, Doerner, Thomas, Dress, Regine J., Dudziak, Diana, Dustin, Michael, Dutertre, Charles-Antoine, Ebner, Friederike, Eckle, Sidonia B. G., Edinger, Matthias, Eede, Pascale, Ehrhardt, Goetz R. A., Eich, Marcus, Engel, Pablo, Engelhardt, Britta, Erdei, Anna, Esser, Charlotte, Everts, Bart, Evrard, Maximilien, Falk, Christine S., Fehniger, Todd A., Felipo-Benavent, Mar, Ferry, Helen, Feuerer, Markus, Filby, Andrew, Filkor, Kata, Fillatreau, Simon, Follo, Marie, Foerster, Irmgard, Foster, John, Foulds, Gemma A., Frehse, Britta, Frenette, Paul S., Frischbutter, Stefan, Fritzsche, Wolfgang, Galbraith, David W., Gangaev, Anastasia, Garbi, Natalio, Gaudilliere, Brice, Gazzinelli, Ricardo T., Geginat, Jens, Gerner, Wilhelm, Gherardin, Nicholas A., Ghoreschi, Kamran, Gibellini, Lara, Ginhoux, Florent, Goda, Keisuke, Godfrey, Dale I., Goettlinger, Christoph, Gonzalez-Navajas, Jose M., Goodyear, Carl S., Gori, Andrea, Grogan, Jane L., Grummitt, Daryl, Gruetzkau, Andreas, Haftmann, Claudia, Hahn, Jonas, Hammad, Hamida, Haemmerling, Guenter, Hansmann, Leo, Hansson, Goran, Harpur, Christopher M., Hartmann, Susanne, Hauser, Andrea, Hauser, Anja E., Haviland, David L., Hedley, David, Hernandez, Daniela C., Herrera, Guadalupe, Herrmann, Martin, Hess, Christoph, Hoefer, Thomas, Hoffmann, Petra, Hogquist, Kristin, Holland, Tristan, Hoellt, Thomas, Holmdahl, Rikard, Hombrink, Pleun, Houston, Jessica P., Hoyer, Bimba F., Huang, Bo, Huang, Fang-Ping, Huber, Johanna E., Huehn, Jochen, Hundemer, Michael, Hunter, Christopher A., Hwang, William Y. K., Iannone, Anna, Ingelfinger, Florian, Ivison, Sabine M., Jaeck, Hans-Martin, Jani, Peter K., Javega, Beatriz, Jonjic, Stipan, Kaiser, Toralf, Kalina, Tomas, Kamradt, Thomas, Kaufmann, Stefan H. E., Keller, Baerbel, Ketelaars, Steven L. C., Khalilnezhad, Ahad, Khan, Srijit, Kisielow, Jan, Klenerman, Paul, Knopf, Jasmin, Koay, Hui-Fern, Kobow, Katja, Kolls, Jay K., Kong, Wan Ting, Kopf, Manfred, Korn, Thomas, Kriegsmann, Katharina, Kristyanto, Hendy, Kroneis, Thomas, Krueger, Andreas, Kuehne, Jenny, Kukat, Christian, Kunkel, Desiree, Kunze-Schumacher, Heike, Kurosaki, Tomohiro, Kurts, Christian, Kvistborg, Pia, Kwok, Immanuel, Landry, Jonathan, Lantz, Olivier, Lanuti, Paola, LaRosa, Francesca, Lehuen, Agnes, LeibundGut-Landmann, Salome, Leipold, Michael D., Leung, Leslie Y. T., Levings, Megan K., Lino, Andreia C., Liotta, Francesco, Litwin, Virginia, Liu, Yanling, Ljunggren, Hans-Gustaf, Lohoff, Michael, Lombardi, Giovanna, Lopez, Lilly, Lopez-Botet, Miguel, Lovett-Racke, Amy E., Lubberts, Erik, Luche, Herve, Ludewig, Burkhard, Lugli, Enrico, Lunemann, Sebastian, Maecker, Holden T., Maggi, Laura, Maguire, Orla, Mair, Florian, Mair, Kerstin H., Mantovani, Alberto, Manz, Rudolf A., Marshall, Aaron J., Martinez-Romero, Alicia, Martrus, Gloria, Marventano, Ivana, Maslinski, Wlodzimierz, Matarese, Giuseppe, Mattioli, Anna Vittoria, Maueroeder, Christian, Mazzoni, Alessio, McCluskey, James, McGrath, Mairi, McGuire, Helen M., McInnes, Iain B., Mei, Henrik E., Melchers, Fritz, Melzer, Susanne, Mielenz, Dirk, Miller, Stephen D., Mills, Kingston H. G., Minderman, Hans, Mjösberg, Jenny, Moore, Jonni, Moran, Barry, Moretta, Lorenzo, Mosmann, Tim R., Mueller, Susann, Multhoff, Gabriele, Munoz, Luis Enrique, Muenz, Christian, Nakayama, Toshinori, Nasi, Milena, Neumann, Katrin, Ng, Lai Guan, Niedobitek, Antonia, Nourshargh, Sussan, Nunez, Gabriel, OConnor, Jose-Enrique, Ochel, Aaron, Oja, Anna, Ordonez, Diana, Orfao, Alberto, Orlowski-Oliver, Eva, Ouyang, Wenjun, Oxenius, Annette, Palankar, Raghavendra, Panse, Isabel, Pattanapanyasat, Kovit, Paulsen, Malte, Pavlinic, Dinko, Penter, Livius, Peterson, Paert, Peth, Christian, Petriz, Jordi, Piancone, Federica, Pickl, Winfried F., Piconese, Silvia, Pinti, Marcello, Pockley, A. Graham, Podolska, Malgorzata Justyna, Poon, Zhiyong, Pracht, Katharina, Prinz, Immo, Pucillo, Carlo E. M., Quataert, Sally A., Quatrini, Linda, Quinn, Kylie M., Radbruch, Helena, Radstake, Tim R. D. J., Rahmig, Susann, Rahn, Hans-Peter, Rajwa, Bartek, Ravichandran, Gevitha, Raz, Yotam, Rebhahn, Jonathan A., Recktenwald, Diether, Reimer, Dorothea, Reis e Sousa, Caetano, Remmerswaal, Ester B. M., Richter, Lisa, Rico, Laura G., Riddell, Andy, Rieger, Aja M., Robinson, J. Paul, Romagnani, Chiara, Rubartelli, Anna, Ruland, Juergen, Saalmueller, Armin, Saeys, Yvan, Saito, Takashi, Sakaguchi, Shimon, Sala-de-Oyanguren, Francisco, Samstag, Yvonne, Sanderson, Sharon, Sandrock, Inga, Santoni, Angela, Sanz, Ramon Bellmas, Saresella, Marina, Sautes-Fridman, Catherine, Sawitzki, Birgit, Schadt, Linda, Scheffold, Alexander, Scherer, Hans U., Schiemann, Matthias, Schildberg, Frank A., Schimisky, Esther, Schlitzer, Andreas, Schlosser, Josephine, Schmid, Stephan, Schmitt, Steffen, Schober, Kilian, Schraivogel, Daniel, Schuh, Wolfgang, Schueler, Thomas, Schulte, Reiner, Schulz, Axel Ronald, Schulz, Sebastian R., Scotta, Cristiano, Scott-Algara, Daniel, Sester, David P., Shankey, T. Vincent, Silva-Santos, Bruno, Simon, Anna Katharina, Sitnik, Katarzyna M., Sozzani, Silvano, Speiser, Daniel E., Spidlen, Josef, Stahlberg, Anders, Stall, Alan M., Stanley, Natalie, Stark, Regina, Stehle, Christina, Steinmetz, Tobit, Stockinger, Hannes, Takahama, Yousuke, Takeda, Kiyoshi, Tan, Leonard, Tarnok, Attila, Tiegs, Gisa, Toldi, Gergely, Tornack, Julia, Traggiai, Elisabetta, Trebak, Mohamed, Tree, Timothy I. M., Trotter, Joe, Trowsdale, John, Tsoumakidou, Maria, Ulrich, Henning, Urbanczyk, Sophia, van de Veen, Willem, van den Broek, Maries, van der Pol, Edwin, Van Gassen, Sofie, Van Isterdael, Gert, van Lier, Rene A. W., Veldhoen, Marc, Vento-Asturias, Salvador, Vieira, Paulo, Voehringer, David, Volk, Hans-Dieter, von Borstel, Anouk, von Volkmann, Konrad, Waisman, Ari, Walker, Rachael V., Wallace, Paul K., Wang, Sa A., Wang, Xin M., Ward, Michael D., Ward-Hartstonge, Kirsten A., Warnatz, Klaus, Warnes, Gary, Warth, Sarah, Waskow, Claudia, Watson, James V., Watzl, Carsten, Wegener, Leonie, Weisenburger, Thomas, Wiedemann, Annika, Wienands, Juergen, Wilharm, Anneke, Wilkinson, Robert John, Willimsky, Gerald, Wing, James B., Winkelmann, Rieke, Winkler, Thomas H., Wirz, Oliver F., Wong, Alicia, Wurst, Peter, Yang, Jennie H. M., Yang, Juhao, Yazdanbakhsh, Maria, Yu, Liping, Yue, Alice, Zhang, Hanlin, Zhao, Yi, Ziegler, Susanne Maria, Zielinski, Christina, Zimmermann, Jakob, Zychlinsky, Arturo, Cossarizza, Andrea, Chang, Hyun-Dong, Radbruch, Andreas, Acs, Andreas, Adam, Dieter, Adam-Klages, Sabine, Agace, William W., Aghaeepour, Nima, Akdis, Muebeccel, Allez, Matthieu, Almeida, Larissa Nogueira, Alvisi, Giorgia, Anderson, Graham, Andrae, Immanuel, Annunziato, Francesco, Anselmo, Achille, Bacher, Petra, Baldari, Cosima T., Bari, Sudipto, Barnaba, Vincenzo, Barros-Martins, Joana, Battistini, Luca, Bauer, Wolfgang, Baumgart, Sabine, Baumgarth, Nicole, Baumjohann, Dirk, Baying, Bianka, Bebawy, Mary, Becher, Burkhard, Beisker, Wolfgang, Benes, Vladimir, Beyaert, Rudi, Blanco, Alfonso, Boardman, Dominic A., Bogdan, Christian, Borger, Jessica G., Borsellino, Giovanna, Boulais, Philip E., Bradford, Jolene A., Brenner, Dirk, Brinkman, Ryan R., Brooks, Anna E. S., Busch, Dirk H., Buescher, Martin, Bushnell, Timothy P., Calzetti, Federica, Cameron, Garth, Cammarata, Ilenia, Cao, Xuetao, Cardell, Susanna L., Casola, Stefano, Cassatella, Marco A., Cavani, Andrea, Celada, Antonio, Chatenoud, Lucienne, Chattopadhyay, Pratip K., Chow, Sue, Christakou, Eleni, Cicin-Sain, Luka, Clerici, Mario, Colombo, Federico S., Cook, Laura, Cooke, Anne, Cooper, Andrea M., Corbett, Alexandra J., Cosma, Antonio, Cosmi, Lorenzo, Coulie, Pierre G., Cumano, Ana, Cvetkovic, Ljiljana, Dang, Van Duc, Dang-Heine, Chantip, Davey, Martin S., Davies, Derek, De Biasi, Sara, Del Zotto, Genny, Dela Cruz, Gelo Victoriano, Delacher, Michael, Della Bella, Silvia, Dellabona, Paolo, Deniz, Guennur, Dessing, Mark, Di Santo, James P., Diefenbach, Andreas, Dieli, Francesco, Dolf, Andreas, Doerner, Thomas, Dress, Regine J., Dudziak, Diana, Dustin, Michael, Dutertre, Charles-Antoine, Ebner, Friederike, Eckle, Sidonia B. G., Edinger, Matthias, Eede, Pascale, Ehrhardt, Goetz R. A., Eich, Marcus, Engel, Pablo, Engelhardt, Britta, Erdei, Anna, Esser, Charlotte, Everts, Bart, Evrard, Maximilien, Falk, Christine S., Fehniger, Todd A., Felipo-Benavent, Mar, Ferry, Helen, Feuerer, Markus, Filby, Andrew, Filkor, Kata, Fillatreau, Simon, Follo, Marie, Foerster, Irmgard, Foster, John, Foulds, Gemma A., Frehse, Britta, Frenette, Paul S., Frischbutter, Stefan, Fritzsche, Wolfgang, Galbraith, David W., Gangaev, Anastasia, Garbi, Natalio, Gaudilliere, Brice, Gazzinelli, Ricardo T., Geginat, Jens, Gerner, Wilhelm, Gherardin, Nicholas A., Ghoreschi, Kamran, Gibellini, Lara, Ginhoux, Florent, Goda, Keisuke, Godfrey, Dale I., Goettlinger, Christoph, Gonzalez-Navajas, Jose M., Goodyear, Carl S., Gori, Andrea, Grogan, Jane L., Grummitt, Daryl, Gruetzkau, Andreas, Haftmann, Claudia, Hahn, Jonas, Hammad, Hamida, Haemmerling, Guenter, Hansmann, Leo, Hansson, Goran, Harpur, Christopher M., Hartmann, Susanne, Hauser, Andrea, Hauser, Anja E., Haviland, David L., Hedley, David, Hernandez, Daniela C., Herrera, Guadalupe, Herrmann, Martin, Hess, Christoph, Hoefer, Thomas, Hoffmann, Petra, Hogquist, Kristin, Holland, Tristan, Hoellt, Thomas, Holmdahl, Rikard, Hombrink, Pleun, Houston, Jessica P., Hoyer, Bimba F., Huang, Bo, Huang, Fang-Ping, Huber, Johanna E., Huehn, Jochen, Hundemer, Michael, Hunter, Christopher A., Hwang, William Y. K., Iannone, Anna, Ingelfinger, Florian, Ivison, Sabine M., Jaeck, Hans-Martin, Jani, Peter K., Javega, Beatriz, Jonjic, Stipan, Kaiser, Toralf, Kalina, Tomas, Kamradt, Thomas, Kaufmann, Stefan H. E., Keller, Baerbel, Ketelaars, Steven L. C., Khalilnezhad, Ahad, Khan, Srijit, Kisielow, Jan, Klenerman, Paul, Knopf, Jasmin, Koay, Hui-Fern, Kobow, Katja, Kolls, Jay K., Kong, Wan Ting, Kopf, Manfred, Korn, Thomas, Kriegsmann, Katharina, Kristyanto, Hendy, Kroneis, Thomas, Krueger, Andreas, Kuehne, Jenny, Kukat, Christian, Kunkel, Desiree, Kunze-Schumacher, Heike, Kurosaki, Tomohiro, Kurts, Christian, Kvistborg, Pia, Kwok, Immanuel, Landry, Jonathan, Lantz, Olivier, Lanuti, Paola, LaRosa, Francesca, Lehuen, Agnes, LeibundGut-Landmann, Salome, Leipold, Michael D., Leung, Leslie Y. T., Levings, Megan K., Lino, Andreia C., Liotta, Francesco, Litwin, Virginia, Liu, Yanling, Ljunggren, Hans-Gustaf, Lohoff, Michael, Lombardi, Giovanna, Lopez, Lilly, Lopez-Botet, Miguel, Lovett-Racke, Amy E., Lubberts, Erik, Luche, Herve, Ludewig, Burkhard, Lugli, Enrico, Lunemann, Sebastian, Maecker, Holden T., Maggi, Laura, Maguire, Orla, Mair, Florian, Mair, Kerstin H., Mantovani, Alberto, Manz, Rudolf A., Marshall, Aaron J., Martinez-Romero, Alicia, Martrus, Gloria, Marventano, Ivana, Maslinski, Wlodzimierz, Matarese, Giuseppe, Mattioli, Anna Vittoria, Maueroeder, Christian, Mazzoni, Alessio, McCluskey, James, McGrath, Mairi, McGuire, Helen M., McInnes, Iain B., Mei, Henrik E., Melchers, Fritz, Melzer, Susanne, Mielenz, Dirk, Miller, Stephen D., Mills, Kingston H. G., Minderman, Hans, Mjösberg, Jenny, Moore, Jonni, Moran, Barry, Moretta, Lorenzo, Mosmann, Tim R., Mueller, Susann, Multhoff, Gabriele, Munoz, Luis Enrique, Muenz, Christian, Nakayama, Toshinori, Nasi, Milena, Neumann, Katrin, Ng, Lai Guan, Niedobitek, Antonia, Nourshargh, Sussan, Nunez, Gabriel, OConnor, Jose-Enrique, Ochel, Aaron, Oja, Anna, Ordonez, Diana, Orfao, Alberto, Orlowski-Oliver, Eva, Ouyang, Wenjun, Oxenius, Annette, Palankar, Raghavendra, Panse, Isabel, Pattanapanyasat, Kovit, Paulsen, Malte, Pavlinic, Dinko, Penter, Livius, Peterson, Paert, Peth, Christian, Petriz, Jordi, Piancone, Federica, Pickl, Winfried F., Piconese, Silvia, Pinti, Marcello, Pockley, A. Graham, Podolska, Malgorzata Justyna, Poon, Zhiyong, Pracht, Katharina, Prinz, Immo, Pucillo, Carlo E. M., Quataert, Sally A., Quatrini, Linda, Quinn, Kylie M., Radbruch, Helena, Radstake, Tim R. D. J., Rahmig, Susann, Rahn, Hans-Peter, Rajwa, Bartek, Ravichandran, Gevitha, Raz, Yotam, Rebhahn, Jonathan A., Recktenwald, Diether, Reimer, Dorothea, Reis e Sousa, Caetano, Remmerswaal, Ester B. M., Richter, Lisa, Rico, Laura G., Riddell, Andy, Rieger, Aja M., Robinson, J. Paul, Romagnani, Chiara, Rubartelli, Anna, Ruland, Juergen, Saalmueller, Armin, Saeys, Yvan, Saito, Takashi, Sakaguchi, Shimon, Sala-de-Oyanguren, Francisco, Samstag, Yvonne, Sanderson, Sharon, Sandrock, Inga, Santoni, Angela, Sanz, Ramon Bellmas, Saresella, Marina, Sautes-Fridman, Catherine, Sawitzki, Birgit, Schadt, Linda, Scheffold, Alexander, Scherer, Hans U., Schiemann, Matthias, Schildberg, Frank A., Schimisky, Esther, Schlitzer, Andreas, Schlosser, Josephine, Schmid, Stephan, Schmitt, Steffen, Schober, Kilian, Schraivogel, Daniel, Schuh, Wolfgang, Schueler, Thomas, Schulte, Reiner, Schulz, Axel Ronald, Schulz, Sebastian R., Scotta, Cristiano, Scott-Algara, Daniel, Sester, David P., Shankey, T. Vincent, Silva-Santos, Bruno, Simon, Anna Katharina, Sitnik, Katarzyna M., Sozzani, Silvano, Speiser, Daniel E., Spidlen, Josef, Stahlberg, Anders, Stall, Alan M., Stanley, Natalie, Stark, Regina, Stehle, Christina, Steinmetz, Tobit, Stockinger, Hannes, Takahama, Yousuke, Takeda, Kiyoshi, Tan, Leonard, Tarnok, Attila, Tiegs, Gisa, Toldi, Gergely, Tornack, Julia, Traggiai, Elisabetta, Trebak, Mohamed, Tree, Timothy I. M., Trotter, Joe, Trowsdale, John, Tsoumakidou, Maria, Ulrich, Henning, Urbanczyk, Sophia, van de Veen, Willem, van den Broek, Maries, van der Pol, Edwin, Van Gassen, Sofie, Van Isterdael, Gert, van Lier, Rene A. W., Veldhoen, Marc, Vento-Asturias, Salvador, Vieira, Paulo, Voehringer, David, Volk, Hans-Dieter, von Borstel, Anouk, von Volkmann, Konrad, Waisman, Ari, Walker, Rachael V., Wallace, Paul K., Wang, Sa A., Wang, Xin M., Ward, Michael D., Ward-Hartstonge, Kirsten A., Warnatz, Klaus, Warnes, Gary, Warth, Sarah, Waskow, Claudia, Watson, James V., Watzl, Carsten, Wegener, Leonie, Weisenburger, Thomas, Wiedemann, Annika, Wienands, Juergen, Wilharm, Anneke, Wilkinson, Robert John, Willimsky, Gerald, Wing, James B., Winkelmann, Rieke, Winkler, Thomas H., Wirz, Oliver F., Wong, Alicia, Wurst, Peter, Yang, Jennie H. M., Yang, Juhao, Yazdanbakhsh, Maria, Yu, Liping, Yue, Alice, Zhang, Hanlin, Zhao, Yi, Ziegler, Susanne Maria, Zielinski, Christina, Zimmermann, Jakob, and Zychlinsky, Arturo
Abstract: These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion., Funding Agencies|Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)German Research Foundation (DFG) [125440785 - SFB 877]; Bavarian Immune Therapy Network (BayImmuNet); Netherlands Organisation for Scientific Research - Domain Applied and Engineering Sciences (NWO-TTW), research program VENI [15924]; DFGGerman Research Foundation (DFG) [MA 2273/14-1]; Excellence Cluster "Inflammation at Interfaces" [EXC 306/2]; German Research FoundationGerman Research Foundation (DFG) [ME 3644/5-1, TRR130 TP24]; NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R50CA211108]; Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [TRR130, C03]; Germany Research FoundationGerman Research Foundation (DFG) [CRC738/B3]; German Rheumatism Research Centre Berlin; European Union Innovative Medicines Initiative - Joint Undertaking [777357]; Else Kroner-Fresenius-Foundation, German Federal Ministry of Education and Research e:Med sysINFLAME Program [01ZX1306B]; KMU-innovativ "InnoCyt"; Leibniz Science Campus; ISAC SRL Emerging Leaders program; European Research Council under the European UnionEuropean Research Council (ERC) [695551]
Published: 2019
Full Text: View/download PDF

23. Probing Transient Valence Orbital Changes with Picosecond Valence-to-Core X-ray Emission Spectroscopy

Author: March, Anne Marie, Assefa, Tadesse A., Boemer, Christina, Bressler, Christian, Britz, Alexander, Diez, Michael, Doumy, Gilles, Galler, Andreas, Harder, Manuel, Khakhulin, Dmitry, Németh, Zoltán, Pápai, Mátyás Imre, Schulz, Sebastian, Southworth, Stephen H., Yavas, Hasan, Young, Linda, Gawelda, Wojciech, Vankó, György, March, Anne Marie, Assefa, Tadesse A., Boemer, Christina, Bressler, Christian, Britz, Alexander, Diez, Michael, Doumy, Gilles, Galler, Andreas, Harder, Manuel, Khakhulin, Dmitry, Németh, Zoltán, Pápai, Mátyás Imre, Schulz, Sebastian, Southworth, Stephen H., Yavas, Hasan, Young, Linda, Gawelda, Wojciech, and Vankó, György
Abstract: We probe the dynamics of valence electrons in photoexcited [Fe(terpy)2]2+ in solution to gain deeper insight into the Fe ligand bond changes. We use hard X-ray emission spectroscopy (XES), which combines element specificity and high penetration with sensitivity to orbital structure, making it a powerful technique for molecular studies in a wide variety of environments. A picosecond-time-resolved measurement of the complete Is X-ray emission spectrum captures the transient photoinduced changes and includes the weak valence-to-core (vtc) emission lines that correspond to transitions from occupied valence orbitals to the nascent core-hole. Vtc-XES offers particular insight into the molecular orbitals directly involved in the light-driven dynamics; a change in the metal ligand orbital overlap results in an intensity reduction and a blue energy shift in agreement with our theoretical calculations and more subtle features at the highest energies reflect changes in the frontier orbital populations.
Published: 2017

24. Pseudomonas aeruginosa LysR PA4203 regulator NmoR acts as a repressor of the PA4202 nmoA gene, encoding a nitronate monooxygenase

Author: Vercammen, Ken, Wei, Qing, Charlier, Daniel, Dötsch, Andreas, Haüssler, Susanne, Schulz, Sebastian, Salvi, Francesca, Gadda, Giovanni, Spain, Jim, Rybtke, Morten Levin, Tolker-Nielsen, Tim, Dingemans, Jozef, Ye, Lumeng, Cornelis, Pierre, Vercammen, Ken, Wei, Qing, Charlier, Daniel, Dötsch, Andreas, Haüssler, Susanne, Schulz, Sebastian, Salvi, Francesca, Gadda, Giovanni, Spain, Jim, Rybtke, Morten Levin, Tolker-Nielsen, Tim, Dingemans, Jozef, Ye, Lumeng, and Cornelis, Pierre
Abstract: The PA4203 gene encodes a LysR regulator and lies between the ppgL gene (PA4204), which encodes a periplasmic gluconolactonase, and, in the opposite orientation, the PA4202 (nmoA) gene, coding for a nitronate monooxygenase, and ddlA (PA4201), encoding a d-alanine alanine ligase. The intergenic regions between PA4203 and ppgL and between PA4203 and nmoA are very short (79 and 107 nucleotides, respectively). Here we show that PA4203 (nmoR) represses its own transcription and the expression of nmoA. A chromatin immunoprecipitation analysis showed the presence of a single NmoR binding site between nmoA and nmoR, which was confirmed by electrophoretic mobility shift assays (EMSAs) with the purified NmoR protein. Despite this observation, a transcriptome analysis revealed more genes to be affected in an nmoR mutant, including genes known to be part of the MexT LysR activator regulon. The PA1225 gene, encoding a quinone oxidoreductase, was the most highly upregulated gene in the nmoR deletion mutant, independently of MexT. Finally, deletion of the nmoA gene resulted in an increased sensitivity of the cells to 3-nitropropionic acid (3-NPA), confirming the role of the nitronate monooxygenase protein in the detoxification of nitronate.
Published: 2015

25. Attenuation of the ELAV1-like protein HuR sensitizes adenocarcinoma cells to the intrinsic apoptotic pathway by increasing the translation of caspase-2L

Author: Winkler, Christoph, Doller, Anke, Imre, Gergely, Badawi, Amel, Schmid, Tobias, Schulz, Sebastian, Steinmeyer, Nico, Pfeilschifter, Josef, Rajalingam, Krishnaraj, Eberhardt, Wolfgang, Winkler, Christoph, Doller, Anke, Imre, Gergely, Badawi, Amel, Schmid, Tobias, Schulz, Sebastian, Steinmeyer, Nico, Pfeilschifter, Josef, Rajalingam, Krishnaraj, and Eberhardt, Wolfgang
Abstract: Caspase-2 represents the most conserved member of the caspase family, which exhibits features of both initiator and effector caspases. Using ribonucleoprotein (RNP)-immunoprecipitation assay, we identified the proapoptotic caspase-2L encoding mRNA as a novel target of the ubiquitous RNA-binding protein HuR in DLD-1 colon carcinoma cells. Unexpectedly, crosslinking-RNP and RNA probe pull-down experiments revealed that HuR binds exclusively to the caspase-2-5' untranslated region (UTR) despite that the 3' UTR of the mRNA bears several adenylate- and uridylate-rich elements representing the prototypical HuR binding sites. By using RNAi-mediated loss-of-function approach, we observed that HuR regulates the mRNA and in turn the protein levels of caspase-2 in a negative manner. Silencing of HuR did not affect the stability of caspase-2 mRNA but resulted in an increased redistribution of caspase-2 transcripts from RNP particles to translational active polysomes implicating that HuR exerts a direct repressive effect on caspase-2 translation. Consistently, in vitro translation of a luciferase reporter gene under the control of an upstream caspase-2-5'UTR was strongly impaired after the addition of recombinant HuR, whereas translation of caspase-2 coding region without the 5'UTR is not affected by HuR confirming the functional role of the caspase-2-5'UTR. Functionally, an elevation in caspase-2 level by HuR knockdown correlated with an increased sensitivity of cells to apoptosis induced by staurosporine- and pore-forming toxins as implicated by their significant accumulation in the sub G1 phase and an increase in caspase-2, -3 and poly ADP-ribose polymerase cleavage, respectively. Importantly, HuR knockdown cells remained insensitive toward STS-induced apoptosis if cells were additionally transfected with caspase-2-specific siRNAs. Collectively, our findings support the hypothesis that HuR by acting as an endogenous inhibitor of caspase-2-driven apoptosis may essentially cont
Published: 2014

26. On the Unlikeliness of Multi-Field Inflation: Bounded Random Potentials and our Vacuum

Author: Battefeld, Diana, Battefeld, Thorsten, Schulz, Sebastian, Battefeld, Diana, Battefeld, Thorsten, and Schulz, Sebastian
Abstract: Based on random matrix theory, we compute the likelihood of saddles and minima in a class of random potentials that are softly bounded from above and below, as required for the validity of low energy effective theories. Imposing this bound leads to a random mass matrix with non-zero mean of its entries. If the dimensionality of field-space is large, inflation is rare, taking place near a saddle point (if at all), since saddles are more likely than minima or maxima for common values of the potential. Due to the boundedness of the potential, the latter become more ubiquitous for rare low/large values respectively. Based on the observation of a positive cosmological constant, we conclude that the dimensionality of field-space after (and most likely during) inflation has to be low if no anthropic arguments are invoked, since the alternative, encountering a metastable deSitter vacuum by chance, is extremely unlikely., Comment: 32 pages, 5 figures; v4: identical to published version (typos corrected)
Published: 2012
Full Text: View/download PDF

27. On the Unlikeliness of Multi-Field Inflation: Bounded Random Potentials and our Vacuum

Author: Battefeld, Diana, Battefeld, Thorsten, Schulz, Sebastian, Battefeld, Diana, Battefeld, Thorsten, and Schulz, Sebastian
Abstract: Based on random matrix theory, we compute the likelihood of saddles and minima in a class of random potentials that are softly bounded from above and below, as required for the validity of low energy effective theories. Imposing this bound leads to a random mass matrix with non-zero mean of its entries. If the dimensionality of field-space is large, inflation is rare, taking place near a saddle point (if at all), since saddles are more likely than minima or maxima for common values of the potential. Due to the boundedness of the potential, the latter become more ubiquitous for rare low/large values respectively. Based on the observation of a positive cosmological constant, we conclude that the dimensionality of field-space after (and most likely during) inflation has to be low if no anthropic arguments are invoked, since the alternative, encountering a metastable deSitter vacuum by chance, is extremely unlikely., Comment: 32 pages, 5 figures; v4: identical to published version (typos corrected)
Published: 2012
Full Text: View/download PDF

28. On the Unlikeliness of Multi-Field Inflation: Bounded Random Potentials and our Vacuum

Author: Battefeld, Diana, Battefeld, Thorsten, Schulz, Sebastian, Battefeld, Diana, Battefeld, Thorsten, and Schulz, Sebastian
Abstract: Based on random matrix theory, we compute the likelihood of saddles and minima in a class of random potentials that are softly bounded from above and below, as required for the validity of low energy effective theories. Imposing this bound leads to a random mass matrix with non-zero mean of its entries. If the dimensionality of field-space is large, inflation is rare, taking place near a saddle point (if at all), since saddles are more likely than minima or maxima for common values of the potential. Due to the boundedness of the potential, the latter become more ubiquitous for rare low/large values respectively. Based on the observation of a positive cosmological constant, we conclude that the dimensionality of field-space after (and most likely during) inflation has to be low if no anthropic arguments are invoked, since the alternative, encountering a metastable deSitter vacuum by chance, is extremely unlikely., Comment: 32 pages, 5 figures; v4: identical to published version (typos corrected)
Published: 2012
Full Text: View/download PDF

29. On the Unlikeliness of Multi-Field Inflation: Bounded Random Potentials and our Vacuum

Author: Battefeld, Diana, Battefeld, Thorsten, Schulz, Sebastian, Battefeld, Diana, Battefeld, Thorsten, and Schulz, Sebastian
Abstract: Based on random matrix theory, we compute the likelihood of saddles and minima in a class of random potentials that are softly bounded from above and below, as required for the validity of low energy effective theories. Imposing this bound leads to a random mass matrix with non-zero mean of its entries. If the dimensionality of field-space is large, inflation is rare, taking place near a saddle point (if at all), since saddles are more likely than minima or maxima for common values of the potential. Due to the boundedness of the potential, the latter become more ubiquitous for rare low/large values respectively. Based on the observation of a positive cosmological constant, we conclude that the dimensionality of field-space after (and most likely during) inflation has to be low if no anthropic arguments are invoked, since the alternative, encountering a metastable deSitter vacuum by chance, is extremely unlikely., Comment: 32 pages, 5 figures; v4: identical to published version (typos corrected)
Published: 2012
Full Text: View/download PDF

30. Ein Hochschulschriftenserver für die SLUB Dresden - Weboberfläche für Browsing und Recherche

Author: Meyer-Wegener, Klaus, Keller, Bernd, Wohlfarth, Dagmar, Grothe, Jürgen, Schulz, Sebastian, Meyer-Wegener, Klaus, Keller, Bernd, Wohlfarth, Dagmar, Grothe, Jürgen, and Schulz, Sebastian
Abstract: In Deutschland stehen vor allem die Universitätsbibliotheken zu Beginn des 21. Jahrhunderts vor der großen Herausforderung, die sich rasch vollziehenden Veränderungen und die sich bietenden technischen Möglichkeiten zu erkennen und als Chance zu begreifen, sich vom Image angestaubter Archivieranstalten zu lösen und sich nach und nach zu ”universitären Informations- und Servicezentren” zu entwickeln. Auch für die Sächsische Landesbibliothek - Staats- und Universitätsbibliothek (SLUB - im Folgenden nur noch SLUB genannt) trifft diese Standortbestimmung zu. Hier machte man sich etwa ab dem Jahr 1999 verstärkt darüber Gedanken, wie man in Zukunft Hochschulschriften digital verwalten und archivieren könnte.
Published: 2006

31. Ein Hochschulschriftenserver für die SLUB Dresden - Weboberfläche für Browsing und Recherche

Author: Meyer-Wegener, Klaus, Keller, Bernd, Wohlfarth, Dagmar, Grothe, Jürgen, Schulz, Sebastian, Meyer-Wegener, Klaus, Keller, Bernd, Wohlfarth, Dagmar, Grothe, Jürgen, and Schulz, Sebastian
Abstract: In Deutschland stehen vor allem die Universitätsbibliotheken zu Beginn des 21. Jahrhunderts vor der großen Herausforderung, die sich rasch vollziehenden Veränderungen und die sich bietenden technischen Möglichkeiten zu erkennen und als Chance zu begreifen, sich vom Image angestaubter Archivieranstalten zu lösen und sich nach und nach zu ”universitären Informations- und Servicezentren” zu entwickeln. Auch für die Sächsische Landesbibliothek - Staats- und Universitätsbibliothek (SLUB - im Folgenden nur noch SLUB genannt) trifft diese Standortbestimmung zu. Hier machte man sich etwa ab dem Jahr 1999 verstärkt darüber Gedanken, wie man in Zukunft Hochschulschriften digital verwalten und archivieren könnte.
Published: 2006

32. Ein Hochschulschriftenserver für die SLUB Dresden - Weboberfläche für Browsing und Recherche

Author: Meyer-Wegener, Klaus, Keller, Bernd, Wohlfarth, Dagmar, Grothe, Jürgen, Schulz, Sebastian, Meyer-Wegener, Klaus, Keller, Bernd, Wohlfarth, Dagmar, Grothe, Jürgen, and Schulz, Sebastian
Abstract: In Deutschland stehen vor allem die Universitätsbibliotheken zu Beginn des 21. Jahrhunderts vor der großen Herausforderung, die sich rasch vollziehenden Veränderungen und die sich bietenden technischen Möglichkeiten zu erkennen und als Chance zu begreifen, sich vom Image angestaubter Archivieranstalten zu lösen und sich nach und nach zu ”universitären Informations- und Servicezentren” zu entwickeln. Auch für die Sächsische Landesbibliothek - Staats- und Universitätsbibliothek (SLUB - im Folgenden nur noch SLUB genannt) trifft diese Standortbestimmung zu. Hier machte man sich etwa ab dem Jahr 1999 verstärkt darüber Gedanken, wie man in Zukunft Hochschulschriften digital verwalten und archivieren könnte.
Published: 2006

33. Ein Hochschulschriftenserver für die SLUB Dresden - Weboberfläche für Browsing und Recherche

Author: Meyer-Wegener, Klaus, Keller, Bernd, Wohlfarth, Dagmar, Grothe, Jürgen, Schulz, Sebastian, Meyer-Wegener, Klaus, Keller, Bernd, Wohlfarth, Dagmar, Grothe, Jürgen, and Schulz, Sebastian
Abstract: In Deutschland stehen vor allem die Universitätsbibliotheken zu Beginn des 21. Jahrhunderts vor der großen Herausforderung, die sich rasch vollziehenden Veränderungen und die sich bietenden technischen Möglichkeiten zu erkennen und als Chance zu begreifen, sich vom Image angestaubter Archivieranstalten zu lösen und sich nach und nach zu ”universitären Informations- und Servicezentren” zu entwickeln. Auch für die Sächsische Landesbibliothek - Staats- und Universitätsbibliothek (SLUB - im Folgenden nur noch SLUB genannt) trifft diese Standortbestimmung zu. Hier machte man sich etwa ab dem Jahr 1999 verstärkt darüber Gedanken, wie man in Zukunft Hochschulschriften digital verwalten und archivieren könnte.
Published: 2006

Catalog

Books, media, physical & digital resources

See catalog results

Searchworks

Select search scope, currently: Articles

Catalog

books, media & more in Jio Institute collections

Articles

journal articles & other e-resources

Refine your results

33 results on '"Schulz, Sebastian"'

1. A Safe Bayesian Optimization Algorithm for Tuning the Optical Synchronization System at European XFEL

2. A case study for measuring the relativistic dipole of a galaxy cross-correlation with the Dark Energy Spectroscopic Instrument

3. Glass-Like Random Catalogues for Two-Point Estimates on the Light Cone

4. Euclid: modelling massive neutrinos in cosmology — a code comparison

5. The halo bias for number counts on the light cone from relativistic N-body simulations

6. Mitochondrial respiration in B lymphocytes is essential for humoral immunity by controlling the flux of the TCA cycle

7. The halo bias for number counts on the light cone from relativistic N-body simulations

8. The dust-continuum size of TNG50 galaxies at z = 1–5: a comparison with the distribution of stellar light, stars, dust, and H2

9. Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

10. The dust-continuum size of TNG50 galaxies at $z=1-5$: a comparison with the distribution of stellar light, stars, dust and H$_2$

11. A pair of noncompeting neutralizing human monoclonal antibodies protecting from disease in a SARS-CoV-2 infection model

12. Escherichia coli Data-Driven Strain Design Using Aggregated Adaptive Laboratory Evolution Mutational Data

13. A redshift-dependent IRX-$\beta$ dust attenuation relation for TNG50 galaxies

14. Über die Veränderung der Insulinsensitivität in gesunden, freiwilligen Probanden nach 14-tägiger Einnahme von Tenofovir alafenamid-haltiger antiretroviraler Medikation

15. Dr Schulz, Sebastian

16. Dr Schulz, Sebastian

17. A redshift-dependent IRX-$\beta$ dust attenuation relation for TNG50 galaxies

18. Über die Veränderung der Insulinsensitivität in gesunden, freiwilligen Probanden nach 14-tägiger Einnahme von Tenofovir alafenamid-haltiger antiretroviraler Medikation

19. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

20. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

21. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

22. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

23. Probing Transient Valence Orbital Changes with Picosecond Valence-to-Core X-ray Emission Spectroscopy

24. Pseudomonas aeruginosa LysR PA4203 regulator NmoR acts as a repressor of the PA4202 nmoA gene, encoding a nitronate monooxygenase

25. Attenuation of the ELAV1-like protein HuR sensitizes adenocarcinoma cells to the intrinsic apoptotic pathway by increasing the translation of caspase-2L

26. On the Unlikeliness of Multi-Field Inflation: Bounded Random Potentials and our Vacuum

27. On the Unlikeliness of Multi-Field Inflation: Bounded Random Potentials and our Vacuum

28. On the Unlikeliness of Multi-Field Inflation: Bounded Random Potentials and our Vacuum

29. On the Unlikeliness of Multi-Field Inflation: Bounded Random Potentials and our Vacuum

30. Ein Hochschulschriftenserver für die SLUB Dresden - Weboberfläche für Browsing und Recherche

31. Ein Hochschulschriftenserver für die SLUB Dresden - Weboberfläche für Browsing und Recherche

32. Ein Hochschulschriftenserver für die SLUB Dresden - Weboberfläche für Browsing und Recherche

33. Ein Hochschulschriftenserver für die SLUB Dresden - Weboberfläche für Browsing und Recherche

Catalog

Searchworks

Select search scope, currently: Articles Catalog books, media & more in Jio Institute collections Articles journal articles & other e-resources

Search

Search Constraints

Refine your results

Search Limiters

Publication Year Range

Publication Type

Database

Publisher

33 results on '"Schulz, Sebastian"'

Search Results

Catalog

Select search scope, currently: Articles

Catalog

books, media & more in Jio Institute collections

Articles

journal articles & other e-resources