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Habitat complexity is widely considered an important determinant of biodiversity, and enhancing complexity can play a key role in restoring degraded habitats. However, the effects of habitat complexity on ecosystem functioning - as opposed to biodiversity and community structure - are relatively poorly understood for artificial habitats, which dominate many coastlines. With Greening of Grey Infrastructure (GGI) approaches, or eco-engineering, increasingly being applied around the globe, it is important to understand the effects that modifying habitat complexity has on both biodiversity and ecological functioning in these highly modified habitats. We assessed how manipulating physical (primary substrate) and/or biogenic habitat (bivalves) complexity on intertidal artificial substrata affected filtration rates, net and gross primary productivity (NPP and GPP, respectively) and community respiration (CR) - as well as abundance of filter feeders and macro-algae and habitat use by cryptobenthic fish across six locations in three continents. We manipulated both physical and biogenic complexity using 1) flat or ridged (2.5 cm or 5 cm) settlement tiles that were either 2) unseeded or seeded with oysters or mussels. Across all locations, increasing physical and biogenic complexity (5 cm seeded tiles) had a significant effect on most ecological functioning variables, increasing overall filtration rates and community respiration of the assemblages on tiles but decreasing productivity (both GPP and NPP) across all locations. There were no overall effects of increasing either type of habitat complexity on cryptobenthic fish MaxN, total time in frame or macro-algal cover. Within each location, there were marked differences in the effects of habitat complexity. In Hobart, we found higher filtration, filter feeder biomass and community respiration on 5 cm tiles compared to flat tiles. However, at this location, both macro-algae cover and GPP decreased with increasing physical compl

Aim Topographic complexity is widely accepted as a key driver of biodiversity, but at the patch‐scale, complexity–biodiversity relationships may vary spatially and temporally according to the environmental stressors complexity mitigates, and the species richness and identity of potential colonists. Using a manipulative experiment, we assessed spatial variation in patch‐scale effects of complexity on intertidal biodiversity. Location 27 sites within 14 estuaries/bays distributed globally. Time period 2015–2017. Major taxa studied Functional groups of algae, sessile and mobile invertebrates. Methods Concrete tiles of differing complexity (flat; 2.5‐cm or 5‐cm complex) were affixed at low–high intertidal elevation on coastal defence structures, and the richness and abundance of the colonizing taxa were quantified after 12 months. Results The patch‐scale effects of complexity varied spatially and among functional groups. Complexity had neutral to positive effects on total, invertebrate and algal taxa richness, and invertebrate abundances. However, effects on the abundance of algae ranged from positive to negative, depending on location and functional group. The tidal elevation at which tiles were placed accounted for some variation. The total and invertebrate richness were greater at low or mid than at high intertidal elevations. Latitude was also an important source of spatial variation, with the effects of complexity on total richness and mobile mollusc abundance greatest at lower latitudes, whilst the cover of sessile invertebrates and sessile molluscs responded most strongly to complexity at higher latitudes. Conclusions After 12 months, patch‐scale relationships between biodiversity and habitat complexity were not universally positive. Instead, the relationship varied among functional groups and according to local abiotic and biotic conditions. This result challenges the assumption that effects of complexity on biodiversity are universally positive. The va

The clinical significance and treatment strategies for minimal acute rejection (grade A1), the most common form of acute rejection (AR), remain controversial. In this retrospective single-center cohort study of 441 lung transplant recipients, we formally evaluate the association between minimal AR and chronic lung allograft dysfunction (CLAD) and test a novel hypothesis using bronchoalveolar lavage (BAL) CXCL9 concentration during minimal AR as a biomarker of subsequent CLAD development. In univariable and multivariable models adjusted for all histopathologic injury patterns, minimal AR was not associated with CLAD development. However, minimal AR with elevated BAL CXCL9 concentrations markedly increased CLAD risk in a dose-response manner. Minimal AR with CXCL9 concentrations greater than the 25th, 50th, and 75th percentile had adjusted hazard ratios (HRs) for CLAD of 1.1 (95% confidence interval [CI] 0.8-1.6), 1.6 (95% CI 1.1-2.3), and 2.2 (95% CI 1.4-3.4), respectively. Thus we demonstrate the utility of BAL CXCL9 measurement as a prognostic biomarker that allows discrimination of recipients at increased risk of CLAD development after minimal AR. BAL CXCL9 measurement during transbronchial biopsies may provide clinically useful prognostic data and guide treatment decisions for this common form of AR, as a possible strategy to minimize CLAD development.

The impact of allograft injury time of onset on the risk of chronic lung allograft dysfunction (CLAD) remains unknown. We hypothesized that episodes of late-onset (≥6 months) allograft injury would produce an augmented CXCR3/ligand immune response, leading to increased CLAD. In a retrospective single-center study, 1894 transbronchial biopsy samples from 441 lung transplant recipients were reviewed for the presence of acute rejection (AR), lymphocytic bronchiolitis (LB), diffuse alveolar damage (DAD), and organizing pneumonia (OP). The association between the time of onset of each injury pattern and CLAD was assessed by using multivariable Cox models with time-dependent covariates. Bronchoalveolar lavage (BAL) CXCR3 ligand concentrations were compared between early- and late-onset injury patterns using linear mixed-effects models. Late-onset DAD and OP were strongly associated with CLAD: adjusted hazard ratio 2.8 (95% confidence interval 1.5-5.3) and 2.0 (1.1-3.4), respectively. The early-onset form of these injury patterns did not increase CLAD risk. Late-onset LB and acute rejection (AR) predicted CLAD in univariable models but lost significance after multivariable adjustment for late DAD and OP. AR was the only early-onset injury pattern associated with CLAD development. Elevated BAL CXCR3 ligand concentrations during late-onset allograft injury parallel the increase in CLAD risk and support our hypothesis that late allograft injuries result in a more profound CXCR3/ligand immune response.

Staphylococcus aureus is the most commonly isolated gram-positive bacterium after lung transplantation (LT) and has been associated with poor posttransplant outcomes, but its effect on bronchiolitis obliterans syndrome (BOS) and death in the context of the allograft inflammatory environment has not been studied. A three-state Cox semi-Markovian model was used to determine the influence of allograft S. aureus and the ELR+ CXC chemokines on the survival rates and cause-specific hazards for movement from lung transplant (State 1) to BOS (State 2), from transplant (State 1) to death (State 3), and from BOS (State 2) to death (State 3). Acute rejection, pseudomonas pneumonia, bronchoalveolar lavage fluid (BALF) CXCL5 and its interaction with S. aureus all increased the likelihood of transition from transplant to BOS. Transition to death from transplant was facilitated by pseudomonas infection and single lung transplant. Movement from BOS to death was affected by the interaction between aspergillus, pseudomonas and CXCL5, but not S. aureus. S. aureus isolation had state specific effects after LT and only in concert with elevated BALF CXCL5 concentrations did it augment the risk of BOS. Pseudomonas and elevated BALF concentrations of CXCL5 continued as significant risk factors for BOS and death after BOS in lung transplantation.

© 2014, Coastal and Estuarine Research Federation. Resilience of seagrass meadows relies on the ability of seagrass to successfully recolonise denuded areas or disperse to new areas. While seed germination and rhizome extension have been explored as modes of recovery and expansion, the contribution of seagrass viviparous propagules to meadow population dynamics has received little attention. Here, we investigated the potential of seagrass viviparous propagules to act as dispersal vectors. We performed a series of density surveys, and in situ and mesocosm-based experiments in Port Phillip Bay, VIC, Australia, using Zostera nigricaulis, a species known to produce viviparous propagules. Production of viviparous propagules was higher at sites with high wind and current exposure, compared to more sheltered environments. A number of propagules remained buoyant and healthy for more than 85 days, suggesting the capacity for relatively long-distance dispersal. Transplanted propagules were found to have improved survivorship within seagrass habitats compared to bare sediment over the short term (4 weeks); however, all propagules suffered longer-term (<100 days) mortality in field experiments. Conditions outside of meadows, including sediment scouring, reduced the likelihood of successful colonisation in bare sediment. Furthermore, sediment characteristics within meadows, such as a smaller grain size and high organic content, positively influenced propagule establishment. This research provides preliminary evidence that propagules have the potential to act as an important long-distance dispersal vector, a process that has previously gone unrecognised. Even though successful establishment of propagules may be rare, viviparous propagules show great potential for seagrass populations given they are facing global decline.

© 2014, Coastal and Estuarine Research Federation. Resilience of seagrass meadows relies on the ability of seagrass to successfully recolonise denuded areas or disperse to new areas. While seed germination and rhizome extension have been explored as modes of recovery and expansion, the contribution of seagrass viviparous propagules to meadow population dynamics has received little attention. Here, we investigated the potential of seagrass viviparous propagules to act as dispersal vectors. We performed a series of density surveys, and in situ and mesocosm-based experiments in Port Phillip Bay, VIC, Australia, using Zostera nigricaulis, a species known to produce viviparous propagules. Production of viviparous propagules was higher at sites with high wind and current exposure, compared to more sheltered environments. A number of propagules remained buoyant and healthy for more than 85 days, suggesting the capacity for relatively long-distance dispersal. Transplanted propagules were found to have improved survivorship within seagrass habitats compared to bare sediment over the short term (4 weeks); however, all propagules suffered longer-term (<100 days) mortality in field experiments. Conditions outside of meadows, including sediment scouring, reduced the likelihood of successful colonisation in bare sediment. Furthermore, sediment characteristics within meadows, such as a smaller grain size and high organic content, positively influenced propagule establishment. This research provides preliminary evidence that propagules have the potential to act as an important long-distance dispersal vector, a process that has previously gone unrecognised. Even though successful establishment of propagules may be rare, viviparous propagules show great potential for seagrass populations given they are facing global decline.

Sediment organic loading has been shown to affect estuarine nitrification and denitrification, resulting in changes to sediment biogeochemistry and nutrient fluxes detrimental to estuarine health. This study examined the effects of organic loading on nutrient fluxes and microbial communities in sediments receiving effluent from a paper and pulp mill (PPM) by applying microcosm studies and molecular microbial ecology techniques. Three sites near the PPM outfall were compared to three control sites, one upstream and two downstream of the outfall. The control sites showed coupled nitrification-denitrification with minimal ammonia release from the sediment. In contrast, the impacted sites were characterised by nitrate uptake and substantial ammonia efflux from the sediments, consistent with a decoupling of nitrification and denitrification. Analysis of gene diversity demonstrated that the composition of nitrifier communities was not significantly different at the impacted sites compared to the control sites; however, analysis of gene abundance indicated that whilst there was no difference in total bacteria, total archaea or ammonia-oxidising archaea (AOA) abundance between the control and impacted sites, there was a significant reduction in ammonia-oxidising bacteria (AOB) at the impacted sites. The results of this study demonstrate an effect of organic loading on estuarine sediment biogeochemistry and highlight an apparent niche differentiation between AOA and AOB.

Seagrasses are marine angiosperms with a worldwide distribution that form conspicuous beds in nearshore habitats. Despite being universally recognised as a foundation species that performs a number of important ecosystems functions (incl. sediment stabilisation, facilitation of biodiversity, nutrient cycling and carbon sequestration), global seagrass habitats are in decline. Resilience-the ability to recover from disturbance without switching to an alternative state-is paramount to the maintenance and persistence of seagrass habitats. Genetic diversity is a key component of seagrass resilience and contributes to an understanding of population structure, connectivity between populations, and reproductive strategies. Microsatellite primers were developed to investigate the resilience of the seagrass Zostera nigricaulis, which dominates subtidal habitats in the bays of south-eastern Australia. We also tested for cross-amplification of markers between Z. nigricaulis and previously developed markers for the sympatric species Z. muelleri to assess their applicability for use in assessing patterns of genetic diversity, population structure, and mating system. Using next-generation sequencing we isolated 11 novel microsatellite loci for Z. nigricaulis, 8 of which were polymorphic for the samples tested. Allelic diversity ranged from 1 to 8. None of the primer pairs developed for Z. nigricaulis cross-amplified in Z. muelleri; but 14 of 24 primer pairs previously developed for Z. muelleri amplified clearly in Z. nigricaulis samples with six of these showing polymorphism. The results demonstrate the applicability of the Z. nigricaulis microsatellite primers for use in the study of population genetics and limited cross-amplification with Z. muelleri. © 2013 Springer Science+Business Media Dordrecht.

Seagrasses are marine angiosperms with a worldwide distribution that form conspicuous beds in nearshore habitats. Despite being universally recognised as a foundation species that performs a number of important ecosystems functions (incl. sediment stabilisation, facilitation of biodiversity, nutrient cycling and carbon sequestration), global seagrass habitats are in decline. Resilience-the ability to recover from disturbance without switching to an alternative state-is paramount to the maintenance and persistence of seagrass habitats. Genetic diversity is a key component of seagrass resilience and contributes to an understanding of population structure, connectivity between populations, and reproductive strategies. Microsatellite primers were developed to investigate the resilience of the seagrass Zostera nigricaulis, which dominates subtidal habitats in the bays of south-eastern Australia. We also tested for cross-amplification of markers between Z. nigricaulis and previously developed markers for the sympatric species Z. muelleri to assess their applicability for use in assessing patterns of genetic diversity, population structure, and mating system. Using next-generation sequencing we isolated 11 novel microsatellite loci for Z. nigricaulis, 8 of which were polymorphic for the samples tested. Allelic diversity ranged from 1 to 8. None of the primer pairs developed for Z. nigricaulis cross-amplified in Z. muelleri; but 14 of 24 primer pairs previously developed for Z. muelleri amplified clearly in Z. nigricaulis samples with six of these showing polymorphism. The results demonstrate the applicability of the Z. nigricaulis microsatellite primers for use in the study of population genetics and limited cross-amplification with Z. muelleri. © 2013 Springer Science+Business Media Dordrecht.

Denitrification is commonly measured using in situ benthic chambers or laboratory incubations of sediment cores. These techniques are similar in principle but differ considerably in cost and practicality. Despite widespread use of both techniques, it is uncertain whether they give comparable results. We compared cores and chambers for measuring fluxes (dissolved oxygen [DO], N 2, NH4+, NO3- and NO 2-) and denitrification efficiency at 2 sites in Port Phillip Bay, Victoria, Australia. Overall, denitrification efficiency was not significantly different between cores and chambers, but fluxes of DO, NO 3- and NO2- differed. Chambers demonstrated higher levels of oxygen consumption and net fluxes of NO 3- and NO2- out of the sediment, suggesting that denitrification and nitrification were closely coupled. In contrast, there was a greater relative importance for uncoupled denitrification in cores as indicated by reduced oxygen consumption and net fluxes of NO 3- into the sediment. We conclude that cores and chambers give different flux results and therefore are not comparable techniques for measuring denitrification. To ascertain the cause of this, we tested the hypothesis that cores failed to adequately incorporate the impacts of macrofauna on fluxes, due to the small size of cores relative to chambers. However, densities of macrofauna were not significantly different in cores and chambers. We then hypothesised that disturbance during core collection, transportation, and handling may account for differences, but cores deployed in situ and in the laboratory gave similar results. We suggest that compression of sediment during insertion of core cylinders into the sediment may account for differences between core and chamber fluxes. © Inter-Research 2006.

Denitrification is commonly measured using in situ benthic chambers or laboratory incubations of sediment cores. These techniques are similar in principle but differ considerably in cost and practicality. Despite widespread use of both techniques, it is uncertain whether they give comparable results. We compared cores and chambers for measuring fluxes (dissolved oxygen [DO], N 2, NH4+, NO3- and NO 2-) and denitrification efficiency at 2 sites in Port Phillip Bay, Victoria, Australia. Overall, denitrification efficiency was not significantly different between cores and chambers, but fluxes of DO, NO 3- and NO2- differed. Chambers demonstrated higher levels of oxygen consumption and net fluxes of NO 3- and NO2- out of the sediment, suggesting that denitrification and nitrification were closely coupled. In contrast, there was a greater relative importance for uncoupled denitrification in cores as indicated by reduced oxygen consumption and net fluxes of NO 3- into the sediment. We conclude that cores and chambers give different flux results and therefore are not comparable techniques for measuring denitrification. To ascertain the cause of this, we tested the hypothesis that cores failed to adequately incorporate the impacts of macrofauna on fluxes, due to the small size of cores relative to chambers. However, densities of macrofauna were not significantly different in cores and chambers. We then hypothesised that disturbance during core collection, transportation, and handling may account for differences, but cores deployed in situ and in the laboratory gave similar results. We suggest that compression of sediment during insertion of core cylinders into the sediment may account for differences between core and chamber fluxes. © Inter-Research 2006.

Escape responses of Australian scallops (Pecten fumatus and Chlamys asperrima) to native and introduced predatory seastars were compared in laboratory and field trials. The native seastar Coscinasterias muricata elicited an almost immediate escape response by scallops in all trials. In contrast, there was a low frequency of escape response exhibited by scallops when held in contact with the introduced seastar Asterias amurensis. These results are consistent with the hypothesis that escape response in invertebrates has evolved relative to predation risk. The absence of predator recognition in marine invertebrates may have serious implications for wild and farmed populations in southern Australia where introduced predators are prevalent.

The northern Pacific seastar, Asterias amurensis, was first collected in southeast Tasmania in 1986. Mistaken for the endemic asteroid Uniophora granifera, its true identity was not realised until 1992. It is now a conspicuous predator in soft sediment habitats in this region, and is considered a major threat to native assemblages and commercial species. We examined the structure of soft sediment assemblages at different spatial scales in southeast Tasmania, and correlated spatial variation in community composition with seastar abundances. We found that the structure of soft sediment assemblages is highly variable at a range of spatial scales from metres to tens of kilometres. Clear differences in the composition of assemblages and abundances of major taxa were detected between areas with and without seastars and between areas with low and high seastar densities. However, the nature of these patterns suggests that they are more likely due to differences in sediment characteristics than due to impacts of the seastar. Thus, spatial differences in soft sediment assemblages might have been erroneously attributed to seastars without detailed information on important physical factors such as sediment characteristics. A second survey, using larger sampling units (1 m2) but across a more limited spatial extent, targeted bivalves and heart urchins that were identified as important prey of the seastar in observations of feeding and in experimental studies. Large-scale patterns of abundance and size structure were consistent with seastar effects anticipated from small-scale experimental and feeding studies for some, but not all, species. While the field survey ultimately provided evidence about the presence or absence of seastar impacts at large-scales, the identification of key ecological variables in experimental and feeding studies proved crucial to both the design and interpretation of patterns observed in the large-scale surveys. Overall, this work highlighted the necessi

Introduced species are having major impacts in terrestrial, freshwater and marine ecosystems worldwide. It is increasingly recognised that effects of multiple species often cannot be predicted from the effect of each species alone, due to complex interactions, but most investigations of invasion impacts have examined only one non-native species at a time and have not addressed the interactive effects of multiple species. We conducted a field experiment to compare the individual and combined effects of two introduced marine predators, the northern Pacific seastar Asterias amurensis and the European green crab Carcinus maenas, on a soft-sediment invertebrate assemblage in Tasmania. Spatial overlap in the distribution of these invaders is just beginning in Tasmania, and appears imminent as their respective ranges expand, suggesting a strong overlap in food resources will result from the shared proclivity for bivalve prey. A. amurensis and C. maenas provide good models to test the interaction between multiple introduced predators, because they leave clear predator-specific traces of their predatory activity for a number of common prey taxa (bivalves and gastropods). Our experiments demonstrate that both predators had a major effect on the abundance of bivalves, reducing populations of the commercial bivalves Fulvia tenuicostata and Katelysia rhytiphora. The interaction between C. maenas and A. amurensis appears to be one of resource competition, resulting in partitioning of bivalves according to size between predators, with A. amurensis consuming the large and C. maenas the small bivalves. At a large spatial scale, we predict that the combined effect on bivalves may be greater than that due to each predator alone simply because their combined distribution is likely to cover a broader range of habitats. At a smaller scale, in the shallow subtidal, where spatial overlap is expected to be most extensive, our results indicate the individual effects of each predator are like

In the past decade there has been a rapid increase in the study of the ecological consequences of marine invasions, but we still have data for only a small proportion of established marine invaders. This is exemplified by Port Phillip Bay, Australia, for which we have quantitative data on the impacts of only a handful of the >160 introduced and cryptogenic species present. Some of the most conspicuous of these invaders are the large epibenthic suspension feeders living on soft sediments. In this study, we examined the impact of 2 of the most common epibenthic suspension feeders in Port Phillip Bay, the introduced polychaete Sabella spallanzanii and an introduced solitary ascidian, Styela clava, by manipulating their densities in the field across the range of naturally occurring densities. Because of their physical presence at the sediment–water interface and suspension feeding activities, we predicted varying impacts across different macrofaunal groups (suspension feeders, deposit feeders, recruits and mobile species). These predictions were not supported for either introduced species despite good power. For individual taxa, there was a significant negative relationship between Sabella density and the abundance of lumbrinerid polychaetes and gammarid amphipods, and between Styela density and the abundance of lumbrinerids, tanaids, crustaceans as a group, and the bivalve Laternula rostrata. Nonetheless, these taxa only represent a small proportion of those present, and importantly, the effects generally emerged at Sabella and Styela densities (>1 to 2 ind. m–2) greater than those typically recorded on Port Phillip Bay sediments. Therefore, we suggest that the effects of Sabella and Styela on soft sediment assemblages in Port Phillip Bay are likely to be negligible.

Introduced species are having major impacts in terrestrial, freshwater, and marine ecosystems worldwide. Given that resources for management are limited and that only a small percentage of invaders are likely to cause large ecological change, management priorities should be based on the severity of immediate and anticipated impacts on native assemblages and commercial species. This paper synthesizes work on the current and predicted impacts of an introduced predatory seastar (Asterias amurensis) on soft sediment assemblages, including native species subject to commercial fishing, in the Derwent Estuary and other areas of southeast Tasmania. Due to the absence of baseline data prior to the arrival of the seastar and the presence of other anthropogenic stressors in the estuary, estimating the impact of the seastar is difficult. To help overcome the weaknesses of any single method, our assessment of impact rests on 'weight of evidence' from multiple approaches. Results from experimental manipulations at small scales, detailed observations of feeding, and field surveys over a range of spatial scales in areas with and without the seastar provide strong evidence that predation by the seastar is likely to be responsible for the decline and subsequent rarity of bivalve species that live just below or on the sediment surface in the Derwent Estuary. The data suggest that should seastar densities in other areas on the Tasmanian coast attain the current levels in the Derwent Estuary, there are likely to be large direct effects on native assemblages, particularly on populations of large surface dwelling bivalves, including several commercial species. Given the seastar's ability to exploit a broad range of food resources other than bivalves, and the functional importance of bivalves in native systems, we predict broader direct and indirect effects on native assemblages. We would be unable to reach these same conclusions from a single approach to assessing impacts. The overall pic

The introduction and establishment of the predatory asteroid Asterias amurensis in coastal waters of southern Australia is considered a major threat to benthic marine assemblages and commercial bivalve species. We compare the impact of the seastar in experiments on three soft-sediment assemblages. The experiments were carried out immediately beyond the current range of the seastar in southeast Tasmania. This allows us to assess the repeatability, and hence predictability, of the type and magnitude of the impact of A. amurensis on soft-sediment assemblages. Responses to manipulations at the species level were dissimilar across the three experiments, reflecting marked initial differences in the three assemblages at both the species and functional group levels. However, at the functional group level, there were notable similarities in the impact of the seastar on the different assemblages. When potential prey taxa were separated into functional groups reflecting ecological availability (e.g. surface dwelling vs. deep burrowing bivalves), seastar effects were largely restricted to the surface bivalves. While the effect of seastar predation on surface bivalves was common in all three assemblages, the magnitude of the impact varied both among and within assemblages. Variability in the impact of A. amurensis at both levels appeared to be largely due to differences in relative availability of prey species. Overall, the results of these experiments indicate that while the impact of the seastar is broadly predictable at the functional group level, the exact nature of seastar effects is likely to be site- and time-specific given the inherent natural variability in soft-sediment assemblages and the seastar's responses to them.

Project Summary PRINCIPAL INVESTIGATOR: Prof. Craig R. Johnson The pattern of distribution of the long-spined sea urchin Centrostephanus rodgersii over ca. 40 y in the Kent group, Bass St., suggests initial establishment in the mid 1960s with subsequent expansion of populations to its current status as the dominant invertebrate on shallow subtidal rocky reef. On the east coast of Tasmania, C. rodgersii is most abundant in the vicinity of its location of initial discovery in 1978, but it occurs throughout the east coast between Eddystone Pt in the north and Recherche Bay in the south. Barrens habitat, supporting high densities of sea urchins but largely devoid of macroalgae, occurs extensively in the Kent group and at several sites on the northern half of the Tasmanian east coast, but declines with increasing latitude and does not occur south of the Tasman Peninsula. At the southern extent of barrens habitat on the open coast, barrens are incipient and occur as small patches in macroalgal beds. Evidence suggests that the barrens habitat in the Kent group and on the open rocky coast of Tasmania is formed by grazing of C. rodgersii and not by Heliociaris erythrogramma, another sea urchin that occurs on these barrens. This is largely because there is a significant positive relationship between C. rodgersii density and extent of barrens but not between H. erythrogramma density and extent of barrens, and because H. erythrogramma is not know to form barrens on exposed coast. These collective patterns suggest that the incursion of C. rodgersii into Tasmanian waters was from the north, and that spread on the east coast of Tasmania propagated from an ‘epicentre’ in the vicinity of St Helens in the northeast. We suggest that the initial incursion was via larvae transported from NSW in the East Australian Current, which has increasingly influenced the east coast of Tasmania over at least the past 4-5 decades. The lack of any genetic differentiation among C. rodgersii population

Escape responses of Australian scallops (Pecten fumatus and Chlamys asperrima) to native and introduced predatory seastars were compared in laboratory and field trials. The native seastar Coscinasterias muricata elicited an almost immediate escape response by scallops in all trials. In contrast, there was a low frequency of escape response exhibited by scallops when held in contact with the introduced seastar Asterias amurensis. These results are consistent with the hypothesis that escape response in invertebrates has evolved relative to predation risk. The absence of predator recognition in marine invertebrates may have serious implications for wild and farmed populations in southern Australia where introduced predators are prevalent.

Project Summary PRINCIPAL INVESTIGATOR: Prof. Craig R. Johnson The pattern of distribution of the long-spined sea urchin Centrostephanus rodgersii over ca. 40 y in the Kent group, Bass St., suggests initial establishment in the mid 1960s with subsequent expansion of populations to its current status as the dominant invertebrate on shallow subtidal rocky reef. On the east coast of Tasmania, C. rodgersii is most abundant in the vicinity of its location of initial discovery in 1978, but it occurs throughout the east coast between Eddystone Pt in the north and Recherche Bay in the south. Barrens habitat, supporting high densities of sea urchins but largely devoid of macroalgae, occurs extensively in the Kent group and at several sites on the northern half of the Tasmanian east coast, but declines with increasing latitude and does not occur south of the Tasman Peninsula. At the southern extent of barrens habitat on the open coast, barrens are incipient and occur as small patches in macroalgal beds. Evidence suggests that the barrens habitat in the Kent group and on the open rocky coast of Tasmania is formed by grazing of C. rodgersii and not by Heliociaris erythrogramma, another sea urchin that occurs on these barrens. This is largely because there is a significant positive relationship between C. rodgersii density and extent of barrens but not between H. erythrogramma density and extent of barrens, and because H. erythrogramma is not know to form barrens on exposed coast. These collective patterns suggest that the incursion of C. rodgersii into Tasmanian waters was from the north, and that spread on the east coast of Tasmania propagated from an ‘epicentre’ in the vicinity of St Helens in the northeast. We suggest that the initial incursion was via larvae transported from NSW in the East Australian Current, which has increasingly influenced the east coast of Tasmania over at least the past 4-5 decades. The lack of any genetic differentiation among C. rodgersii population

In the past decade there has been a rapid increase in the study of the ecological consequences of marine invasions, but we still have data for only a small proportion of established marine invaders. This is exemplified by Port Phillip Bay, Australia, for which we have quantitative data on the impacts of only a handful of the >160 introduced and cryptogenic species present. Some of the most conspicuous of these invaders are the large epibenthic suspension feeders living on soft sediments. In this study, we examined the impact of 2 of the most common epibenthic suspension feeders in Port Phillip Bay, the introduced polychaete Sabella spallanzanii and an introduced solitary ascidian, Styela clava, by manipulating their densities in the field across the range of naturally occurring densities. Because of their physical presence at the sediment–water interface and suspension feeding activities, we predicted varying impacts across different macrofaunal groups (suspension feeders, deposit feeders, recruits and mobile species). These predictions were not supported for either introduced species despite good power. For individual taxa, there was a significant negative relationship between Sabella density and the abundance of lumbrinerid polychaetes and gammarid amphipods, and between Styela density and the abundance of lumbrinerids, tanaids, crustaceans as a group, and the bivalve Laternula rostrata. Nonetheless, these taxa only represent a small proportion of those present, and importantly, the effects generally emerged at Sabella and Styela densities (>1 to 2 ind. m–2) greater than those typically recorded on Port Phillip Bay sediments. Therefore, we suggest that the effects of Sabella and Styela on soft sediment assemblages in Port Phillip Bay are likely to be negligible.

Introduced species are having major impacts in terrestrial, freshwater, and marine ecosystems worldwide. Given that resources for management are limited and that only a small percentage of invaders are likely to cause large ecological change, management priorities should be based on the severity of immediate and anticipated impacts on native assemblages and commercial species. This paper synthesizes work on the current and predicted impacts of an introduced predatory seastar (Asterias amurensis) on soft sediment assemblages, including native species subject to commercial fishing, in the Derwent Estuary and other areas of southeast Tasmania. Due to the absence of baseline data prior to the arrival of the seastar and the presence of other anthropogenic stressors in the estuary, estimating the impact of the seastar is difficult. To help overcome the weaknesses of any single method, our assessment of impact rests on 'weight of evidence' from multiple approaches. Results from experimental manipulations at small scales, detailed observations of feeding, and field surveys over a range of spatial scales in areas with and without the seastar provide strong evidence that predation by the seastar is likely to be responsible for the decline and subsequent rarity of bivalve species that live just below or on the sediment surface in the Derwent Estuary. The data suggest that should seastar densities in other areas on the Tasmanian coast attain the current levels in the Derwent Estuary, there are likely to be large direct effects on native assemblages, particularly on populations of large surface dwelling bivalves, including several commercial species. Given the seastar's ability to exploit a broad range of food resources other than bivalves, and the functional importance of bivalves in native systems, we predict broader direct and indirect effects on native assemblages. We would be unable to reach these same conclusions from a single approach to assessing impacts. The overall pic

The introduction and establishment of the predatory asteroid Asterias amurensis in coastal waters of southern Australia is considered a major threat to benthic marine assemblages and commercial bivalve species. We compare the impact of the seastar in experiments on three soft-sediment assemblages. The experiments were carried out immediately beyond the current range of the seastar in southeast Tasmania. This allows us to assess the repeatability, and hence predictability, of the type and magnitude of the impact of A. amurensis on soft-sediment assemblages. Responses to manipulations at the species level were dissimilar across the three experiments, reflecting marked initial differences in the three assemblages at both the species and functional group levels. However, at the functional group level, there were notable similarities in the impact of the seastar on the different assemblages. When potential prey taxa were separated into functional groups reflecting ecological availability (e.g. surface dwelling vs. deep burrowing bivalves), seastar effects were largely restricted to the surface bivalves. While the effect of seastar predation on surface bivalves was common in all three assemblages, the magnitude of the impact varied both among and within assemblages. Variability in the impact of A. amurensis at both levels appeared to be largely due to differences in relative availability of prey species. Overall, the results of these experiments indicate that while the impact of the seastar is broadly predictable at the functional group level, the exact nature of seastar effects is likely to be site- and time-specific given the inherent natural variability in soft-sediment assemblages and the seastar's responses to them.

The northern Pacific seastar, Asterias amurensis, was first collected in southeast Tasmania in 1986. Mistaken for the endemic asteroid Uniophora granifera, its true identity was not realised until 1992. It is now a conspicuous predator in soft sediment habitats in this region, and is considered a major threat to native assemblages and commercial species. We examined the structure of soft sediment assemblages at different spatial scales in southeast Tasmania, and correlated spatial variation in community composition with seastar abundances. We found that the structure of soft sediment assemblages is highly variable at a range of spatial scales from metres to tens of kilometres. Clear differences in the composition of assemblages and abundances of major taxa were detected between areas with and without seastars and between areas with low and high seastar densities. However, the nature of these patterns suggests that they are more likely due to differences in sediment characteristics than due to impacts of the seastar. Thus, spatial differences in soft sediment assemblages might have been erroneously attributed to seastars without detailed information on important physical factors such as sediment characteristics. A second survey, using larger sampling units (1 m2) but across a more limited spatial extent, targeted bivalves and heart urchins that were identified as important prey of the seastar in observations of feeding and in experimental studies. Large-scale patterns of abundance and size structure were consistent with seastar effects anticipated from small-scale experimental and feeding studies for some, but not all, species. While the field survey ultimately provided evidence about the presence or absence of seastar impacts at large-scales, the identification of key ecological variables in experimental and feeding studies proved crucial to both the design and interpretation of patterns observed in the large-scale surveys. Overall, this work highlighted the necessi

Introduced species are having major impacts in terrestrial, freshwater and marine ecosystems worldwide. It is increasingly recognised that effects of multiple species often cannot be predicted from the effect of each species alone, due to complex interactions, but most investigations of invasion impacts have examined only one non-native species at a time and have not addressed the interactive effects of multiple species. We conducted a field experiment to compare the individual and combined effects of two introduced marine predators, the northern Pacific seastar Asterias amurensis and the European green crab Carcinus maenas, on a soft-sediment invertebrate assemblage in Tasmania. Spatial overlap in the distribution of these invaders is just beginning in Tasmania, and appears imminent as their respective ranges expand, suggesting a strong overlap in food resources will result from the shared proclivity for bivalve prey. A. amurensis and C. maenas provide good models to test the interaction between multiple introduced predators, because they leave clear predator-specific traces of their predatory activity for a number of common prey taxa (bivalves and gastropods). Our experiments demonstrate that both predators had a major effect on the abundance of bivalves, reducing populations of the commercial bivalves Fulvia tenuicostata and Katelysia rhytiphora. The interaction between C. maenas and A. amurensis appears to be one of resource competition, resulting in partitioning of bivalves according to size between predators, with A. amurensis consuming the large and C. maenas the small bivalves. At a large spatial scale, we predict that the combined effect on bivalves may be greater than that due to each predator alone simply because their combined distribution is likely to cover a broader range of habitats. At a smaller scale, in the shallow subtidal, where spatial overlap is expected to be most extensive, our results indicate the individual effects of each predator are like