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1. Labeling of Bifidobacterium longum Cells with 13C-Substituted Leucine for Quantitative Proteomic Analyses

Author

European Commission, Comisión Interministerial de Ciencia y Tecnología, CICYT (España), Swiss National Science Foundation, Couté, Yohann, Hernandez, Céline, Ron D., Appel, Sanchez, Jean-Charles, Margolles Barros, Abelardo, European Commission, Comisión Interministerial de Ciencia y Tecnología, CICYT (España), Swiss National Science Foundation, Couté, Yohann, Hernandez, Céline, Ron D., Appel, Sanchez, Jean-Charles, and Margolles Barros, Abelardo

Abstract

Stable isotope labeling of amino acids in cell culture was used for Bifidobacterium longum. A comprehensive proteomic strategy was developed and validated by designing an appropriate semidefined medium that allows stable replacement of natural leucine by [13C6]leucine. Using this strategy, proteins having variations of at least 50% in their expression rates can be quantified with great confidence

Published
2007

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