48 results on '"Ribeiro-Dias F"'
Search Results
2. Protective immune response mediated by neutrophils in experimental visceral leishmaniasis is enhanced by IL-32 gamma
- Author
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Gomes, R.S., Teodoro Silva, Muriel Vilela, Pelli Oliveira, Milton Adriano, Joosten, L.A.B., Ribeiro-Dias, F., Gomes, R.S., Teodoro Silva, Muriel Vilela, Pelli Oliveira, Milton Adriano, Joosten, L.A.B., and Ribeiro-Dias, F.
- Abstract
Item does not contain fulltext
- Published
- 2022
3. The role of IL-32 in Bacillus Calmette-Guérin (BCG)-induced trained immunity in infections caused by different Leishmania spp
- Author
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Silva, M.V.T., Dos Santos, J.C., Figueiredo, A.M.B., Teufel, L.U., Pereira, J.X., Matos, G.G., Pinto, S.A., Netea, M.G., Gomes, R.S., Joosten, L.A.B., Ribeiro-Dias, F., Silva, M.V.T., Dos Santos, J.C., Figueiredo, A.M.B., Teufel, L.U., Pereira, J.X., Matos, G.G., Pinto, S.A., Netea, M.G., Gomes, R.S., Joosten, L.A.B., and Ribeiro-Dias, F.
- Abstract
Item does not contain fulltext, BACKGROUND: Cells of the innate immune system undergo long-term functional reprogramming in response to Bacillus Calmette-Guérin (BCG) exposure via a process called trained immunity, conferring nonspecific protection to unrelated infections. Here, we investigate whether BCG-induced trained immunity is able to protect against infections caused by different Leishmania spp., protozoa that cause cutaneous and mucosal or visceral lesions. METHODS: We used training models of human monocytes with BCG and subsequent infection by L. braziliensis, L. amazonensis and L. infantum, and the vaccination of wild-type and transgenic mice for IL-32γ before in vivo challenge with parasites. RESULTS: We demonstrated that monocytes trained with BCG presented enhanced ability to kill L. braziliensis, L. amazonensis and L. infantum through increased production of reactive oxygen species. Interleukin (IL)-32 appears to play an essential role in the development of trained immunity. Indeed, BCG exposure induced IL-32 production in human primary monocytes, both mRNA and protein. We have used a human IL-32γ transgenic mouse model (IL-32γTg) to study the effect of BCG vaccination in different Leishmania infection models. BCG vaccination decreased lesion size and parasite load in infections caused by L. braziliensis and reduced the spread of L. amazonensis to other organs in both infected wild-type (WT) and IL-32γTg mice. In addition, BCG reduced the parasite load in the spleen, liver and bone marrow of both WT and IL-32γTg mice infected with L. infantum. BCG vaccination increased inflammatory infiltrate in infected tissues caused by different Leishmania spp. In all infections, the presence of IL-32γ was not mandatory, but it increased the protective and inflammatory effects of BCG-induced training. CONCLUSIONS: BCG's ability to train innate immune cells, providing protection against leishmaniasis, as well as the participation of IL-32γ in this process, pave the way for new treatment strategies
- Published
- 2021
4. Paracoccidioides brasiliensis induces IL-32 and is controlled by IL-15/IL-32/ vitamin D pathway in vitro
- Author
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Matos, Grazzielle Guimaraes de, Barroso de Figueiredo, Ana Marina, Diniz Goncalves, Pedro Hugo, Lima Silva, Lucas Luiz de, Bastista, Aline Carvalho, Borges, Clayton Luiz, Joosten, L.A.B., Ribeiro-Dias, F., Matos, Grazzielle Guimaraes de, Barroso de Figueiredo, Ana Marina, Diniz Goncalves, Pedro Hugo, Lima Silva, Lucas Luiz de, Bastista, Aline Carvalho, Borges, Clayton Luiz, Joosten, L.A.B., and Ribeiro-Dias, F.
- Abstract
Item does not contain fulltext
- Published
- 2021
5. Paracoccidioides brasiliensis induces IL-32 and is controlled by IL-15/IL-32/ vitamin D pathway in vitro
- Author
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Matos, Grazzielle Guimaraes de, Barroso de Figueiredo, Ana Marina, Diniz Goncalves, Pedro Hugo, Lima Silva, Lucas Luiz de, Bastista, Aline Carvalho, Borges, Clayton Luiz, Joosten, L.A.B., Ribeiro-Dias, F., Matos, Grazzielle Guimaraes de, Barroso de Figueiredo, Ana Marina, Diniz Goncalves, Pedro Hugo, Lima Silva, Lucas Luiz de, Bastista, Aline Carvalho, Borges, Clayton Luiz, Joosten, L.A.B., and Ribeiro-Dias, F.
- Abstract
Item does not contain fulltext
- Published
- 2021
6. Genetic variation in Interleukin-32 influence the immune response against New World Leishmania species and susceptibility to American Tegumentary Leishmaniasis
- Author
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Dos Santos, J.C., Leite Quixabeira, Valeria Bernadete, Teodoro Silva, Muriel Vilela, Damen, M.S.M.A., Schraa, K., Jaeger, M., Oosting, M., Keating, S.T., Netea, M.G., Ribeiro-Dias, F., Joosten, L.A., Dos Santos, J.C., Leite Quixabeira, Valeria Bernadete, Teodoro Silva, Muriel Vilela, Damen, M.S.M.A., Schraa, K., Jaeger, M., Oosting, M., Keating, S.T., Netea, M.G., Ribeiro-Dias, F., and Joosten, L.A.
- Abstract
Contains fulltext : 217639.pdf (publisher's version ) (Open Access)
- Published
- 2020
7. IL-15 enhances the capacity of primary human macrophages to control Leishmania braziliensis infection by IL-32/vitamin D dependent and independent pathways
- Author
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Lima Silva, Lucas Luiz de, Gomes, R.S., Teodoro Silva, Muriel Vilela, Joosten, L.A.B., Ribeiro-Dias, F., Lima Silva, Lucas Luiz de, Gomes, R.S., Teodoro Silva, Muriel Vilela, Joosten, L.A.B., and Ribeiro-Dias, F.
- Abstract
Item does not contain fulltext
- Published
- 2020
8. Genetic variation in Interleukin-32 influence the immune response against New World Leishmania species and susceptibility to American Tegumentary Leishmaniasis
- Author
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Dos Santos, J.C., Leite Quixabeira, Valeria Bernadete, Teodoro Silva, Muriel Vilela, Damen, M.S.M.A., Schraa, K., Jaeger, M., Oosting, M., Keating, S.T., Netea, M.G., Ribeiro-Dias, F., Joosten, L.A., Dos Santos, J.C., Leite Quixabeira, Valeria Bernadete, Teodoro Silva, Muriel Vilela, Damen, M.S.M.A., Schraa, K., Jaeger, M., Oosting, M., Keating, S.T., Netea, M.G., Ribeiro-Dias, F., and Joosten, L.A.
- Abstract
Contains fulltext : 217639.pdf (publisher's version ) (Open Access)
- Published
- 2020
9. IL-15 enhances the capacity of primary human macrophages to control Leishmania braziliensis infection by IL-32/vitamin D dependent and independent pathways
- Author
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Lima Silva, Lucas Luiz de, Gomes, R.S., Teodoro Silva, Muriel Vilela, Joosten, L.A.B., Ribeiro-Dias, F., Lima Silva, Lucas Luiz de, Gomes, R.S., Teodoro Silva, Muriel Vilela, Joosten, L.A.B., and Ribeiro-Dias, F.
- Abstract
Item does not contain fulltext
- Published
- 2020
10. IL-15 enhances the capacity of primary human macrophages to control Leishmania braziliensis infection by IL-32/vitamin D dependent and independent pathways
- Author
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Lima Silva, Lucas Luiz de, Gomes, R.S., Teodoro Silva, Muriel Vilela, Joosten, L.A.B., Ribeiro-Dias, F., Lima Silva, Lucas Luiz de, Gomes, R.S., Teodoro Silva, Muriel Vilela, Joosten, L.A.B., and Ribeiro-Dias, F.
- Abstract
Item does not contain fulltext
- Published
- 2020
11. Genetic variation in Interleukin-32 influence the immune response against New World Leishmania species and susceptibility to American Tegumentary Leishmaniasis
- Author
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Dos Santos, J.C., Leite Quixabeira, Valeria Bernadete, Teodoro Silva, Muriel Vilela, Damen, M.S.M.A., Schraa, K., Jaeger, M., Oosting, M., Keating, S.T., Netea, M.G., Ribeiro-Dias, F., Joosten, L.A., Dos Santos, J.C., Leite Quixabeira, Valeria Bernadete, Teodoro Silva, Muriel Vilela, Damen, M.S.M.A., Schraa, K., Jaeger, M., Oosting, M., Keating, S.T., Netea, M.G., Ribeiro-Dias, F., and Joosten, L.A.
- Abstract
Contains fulltext : 217639.pdf (publisher's version ) (Open Access)
- Published
- 2020
12. Non-specific effects of BCG in protozoal infections: tegumentary leishmaniasis and malaria
- Author
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Dos Santos, J.C., Silva, M., Ribeiro-Dias, F., Joosten, L.A.B., Dos Santos, J.C., Silva, M., Ribeiro-Dias, F., and Joosten, L.A.B.
- Abstract
Item does not contain fulltext, BACKGROUND: Leishmaniasis and malaria are major causes of illness in the poorest countries. In the absence of efficient strategies to prevent infections and to control the transmission of the parasites by insect vectors, treatment relies on drug therapy. Vaccine development continues on several fronts; however none of the candidates developed has so far been shown to provide long-lasting protection at a population level. Because the bacillus Calmette-Guerin (BCG) vaccine can induce heterologous protective effects, we hypothesize that BCG has beneficial effects in the control of tegumentary leishmaniasis (TL) and malaria. AIMS: In this review we describe evidence for the protective efficacy of BCG against tegumentary leishmaniasis and malaria in humans. SOURCES: Relevant data from peer-reviewed scientific literature published on Pubmed up to January 2019 were examined. CONTENT: From experimental animal and various human studies with BCG and one recent randomized malaria trial there is evidence that BCG has beneficial effects in Leishmania spp. and Plasmodium falciparum infections. Although the precise mechanisms remain unknown, BCG can activate innate immune responses, and an increasing body of evidence demonstrates that the induction of trained innate immunity could explain its non-specific protective effects. IMPLICATIONS: Despite many years of research to prevent and treat TL and malaria, these diseases remain a public health problem in tropical countries. Future studies are required to examine if BCG vaccination could be used as an effective treatment option.
- Published
- 2019
13. beta-Glucan-Induced Trained Immunity Protects against Leishmania braziliensis Infection: a Crucial Role for IL-32
- Author
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Dos Santos, J.C., Barroso de Figueiredo, Ana Marina, Teodoro Silva, Muriel Vilela, Cirovic, Branko, Bree, L.C.J. de, Damen, M.S.M.A., Moorlag, S.J.C.F.M., Helsen, M.M.A., Oosting, M., Keating, S.T., Netea, M.G., Ribeiro-Dias, F., Joosten, L.A.B., Dos Santos, J.C., Barroso de Figueiredo, Ana Marina, Teodoro Silva, Muriel Vilela, Cirovic, Branko, Bree, L.C.J. de, Damen, M.S.M.A., Moorlag, S.J.C.F.M., Helsen, M.M.A., Oosting, M., Keating, S.T., Netea, M.G., Ribeiro-Dias, F., and Joosten, L.A.B.
- Abstract
Contains fulltext : 207901.pdf (publisher's version ) (Open Access)
- Published
- 2019
14. The role of interleukin-32 in infections caused by New World Leishmania species and identification of novel treatment strategies
- Author
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Joosten, L.A.B., Ribeiro Dias, F., Dos Santos, J.C., Joosten, L.A.B., Ribeiro Dias, F., and Dos Santos, J.C.
- Abstract
Radboud University, 28 februari 2019, Promotores : Joosten, L.A.B., Ribeiro Dias, F., Contains fulltext : 200683.pdf (publisher's version ) (Open Access)
- Published
- 2019
15. Non-specific effects of BCG in protozoal infections: tegumentary leishmaniasis and malaria
- Author
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Dos Santos, J.C., Silva, M., Ribeiro-Dias, F., Joosten, L.A.B., Dos Santos, J.C., Silva, M., Ribeiro-Dias, F., and Joosten, L.A.B.
- Abstract
Item does not contain fulltext, BACKGROUND: Leishmaniasis and malaria are major causes of illness in the poorest countries. In the absence of efficient strategies to prevent infections and to control the transmission of the parasites by insect vectors, treatment relies on drug therapy. Vaccine development continues on several fronts; however none of the candidates developed has so far been shown to provide long-lasting protection at a population level. Because the bacillus Calmette-Guerin (BCG) vaccine can induce heterologous protective effects, we hypothesize that BCG has beneficial effects in the control of tegumentary leishmaniasis (TL) and malaria. AIMS: In this review we describe evidence for the protective efficacy of BCG against tegumentary leishmaniasis and malaria in humans. SOURCES: Relevant data from peer-reviewed scientific literature published on Pubmed up to January 2019 were examined. CONTENT: From experimental animal and various human studies with BCG and one recent randomized malaria trial there is evidence that BCG has beneficial effects in Leishmania spp. and Plasmodium falciparum infections. Although the precise mechanisms remain unknown, BCG can activate innate immune responses, and an increasing body of evidence demonstrates that the induction of trained innate immunity could explain its non-specific protective effects. IMPLICATIONS: Despite many years of research to prevent and treat TL and malaria, these diseases remain a public health problem in tropical countries. Future studies are required to examine if BCG vaccination could be used as an effective treatment option.
- Published
- 2019
16. beta-Glucan-Induced Trained Immunity Protects against Leishmania braziliensis Infection: a Crucial Role for IL-32
- Author
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Dos Santos, J.C., Barroso de Figueiredo, Ana Marina, Teodoro Silva, Muriel Vilela, Cirovic, Branko, Bree, L.C.J. de, Damen, M.S.M.A., Moorlag, S.J.C.F.M., Helsen, M.M.A., Oosting, M., Keating, S.T., Netea, M.G., Ribeiro-Dias, F., Joosten, L.A.B., Dos Santos, J.C., Barroso de Figueiredo, Ana Marina, Teodoro Silva, Muriel Vilela, Cirovic, Branko, Bree, L.C.J. de, Damen, M.S.M.A., Moorlag, S.J.C.F.M., Helsen, M.M.A., Oosting, M., Keating, S.T., Netea, M.G., Ribeiro-Dias, F., and Joosten, L.A.B.
- Abstract
Contains fulltext : 207901.pdf (publisher's version ) (Open Access)
- Published
- 2019
17. The role of interleukin-32 in infections caused by New World Leishmania species and identification of novel treatment strategies
- Author
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Joosten, L.A.B., Ribeiro Dias, F., Dos Santos, J.C., Joosten, L.A.B., Ribeiro Dias, F., and Dos Santos, J.C.
- Abstract
Radboud University, 28 februari 2019, Promotores : Joosten, L.A.B., Ribeiro Dias, F., Contains fulltext : 200683.pdf (publisher's version ) (Open Access)
- Published
- 2019
18. The role of interleukin-32 in infections caused by New World Leishmania species and identification of novel treatment strategies
- Author
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Joosten, L.A.B., Ribeiro Dias, F., Dos Santos, J.C., Joosten, L.A.B., Ribeiro Dias, F., and Dos Santos, J.C.
- Abstract
Radboud University, 28 februari 2019, Promotores : Joosten, L.A.B., Ribeiro Dias, F., Contains fulltext : 200683.pdf (publisher's version ) (Open Access)
- Published
- 2019
19. beta-Glucan-Induced Trained Immunity Protects against Leishmania braziliensis Infection: a Crucial Role for IL-32
- Author
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Dos Santos, J.C., Barroso de Figueiredo, Ana Marina, Teodoro Silva, Muriel Vilela, Cirovic, Branko, Bree, L.C.J. de, Damen, M.S.M.A., Moorlag, S.J.C.F.M., Helsen, M.M.A., Oosting, M., Keating, S.T., Netea, M.G., Ribeiro-Dias, F., Joosten, L.A.B., Dos Santos, J.C., Barroso de Figueiredo, Ana Marina, Teodoro Silva, Muriel Vilela, Cirovic, Branko, Bree, L.C.J. de, Damen, M.S.M.A., Moorlag, S.J.C.F.M., Helsen, M.M.A., Oosting, M., Keating, S.T., Netea, M.G., Ribeiro-Dias, F., and Joosten, L.A.B.
- Abstract
Contains fulltext : 207901.pdf (publisher's version ) (Open Access)
- Published
- 2019
20. Human Interleukin-32 gamma Plays a Protective Role in an Experimental Model of Visceral Leishmaniasis in Mice
- Author
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Gomes, R.S., Teodoro Silva, Muriel Vilela, Dos Santos, J.C., Linge, Christine van, Reis, Juliana Machado, Teixeira, M.M., Dinarello, C.A., Joosten, L.A.B., Ribeiro-Dias, F., Gomes, R.S., Teodoro Silva, Muriel Vilela, Dos Santos, J.C., Linge, Christine van, Reis, Juliana Machado, Teixeira, M.M., Dinarello, C.A., Joosten, L.A.B., and Ribeiro-Dias, F.
- Abstract
Contains fulltext : 191165.pdf (publisher's version ) (Closed access)
- Published
- 2018
21. Differential In Vitro Cytokine Induction by the Species of Cryptococcus gattii Complex
- Author
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Herkert, P.F., Dos Santos, J.C., Hagen, F., Ribeiro-Dias, F., Queiroz-Telles, F., Netea, M.G., Meis, J.F., Joosten, L.A.B., Herkert, P.F., Dos Santos, J.C., Hagen, F., Ribeiro-Dias, F., Queiroz-Telles, F., Netea, M.G., Meis, J.F., and Joosten, L.A.B.
- Abstract
Contains fulltext : 190812.pdf (publisher's version ) (Closed access), Cryptococcal species vary in capsule and cell size, thermotolerance, geographic distribution, and affected populations. Cryptococcus gattii sensu stricto and C. deuterogattii affect mainly immunocompetent hosts; however, C. bacillisporus, C. decagattii, and C. tetragattii cause infections mainly in immunocompromised hosts. This study aimed to compare the capacities of different species of the C. gattii species complex to induce cytokines and antimicrobial molecules in human peripheral blood mononuclear cells (PBMCs). Cryptococcus bacillisporus and C. deuterogattii induced the lowest levels of tumor necrosis factor alpha (TNF-alpha), interleukin-1beta (IL-1beta), and IL-6 among the five species of the C. gattii complex. Cryptococcus deuterogattii induced higher levels of IL-22 than those induced by C. tetragattii and the environmental species C. flavescens In addition, C. bacillisporus and C. gattii sensu stricto proliferated inside human monocyte-derived macrophages after 24 h of infection. All Cryptococcus species were able to generate reactive oxygen species (ROS) in human PBMCs, with C. bacillisporus and C. deuterogattii being more efficient than the other species. In conclusion, C. bacillisporus and C. deuterogattii induce lower levels of the proinflammatory cytokines TNF-alpha, IL-1beta, and IL-6 and higher ROS levels than those induced by the other species. Species of the Cryptococcus gattii complex have different abilities to induce cytokine and ROS production by human PBMCs.
- Published
- 2018
22. Human Interleukin-32 gamma Plays a Protective Role in an Experimental Model of Visceral Leishmaniasis in Mice
- Author
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Gomes, R.S., Teodoro Silva, Muriel Vilela, Dos Santos, J.C., Linge, Christine van, Reis, Juliana Machado, Teixeira, M.M., Dinarello, C.A., Joosten, L.A.B., Ribeiro-Dias, F., Gomes, R.S., Teodoro Silva, Muriel Vilela, Dos Santos, J.C., Linge, Christine van, Reis, Juliana Machado, Teixeira, M.M., Dinarello, C.A., Joosten, L.A.B., and Ribeiro-Dias, F.
- Abstract
Contains fulltext : 191165.pdf (publisher's version ) (Closed access)
- Published
- 2018
23. Differential In Vitro Cytokine Induction by the Species of Cryptococcus gattii Complex
- Author
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Herkert, P.F., Dos Santos, J.C., Hagen, F., Ribeiro-Dias, F., Queiroz-Telles, F., Netea, M.G., Meis, J.F., Joosten, L.A.B., Herkert, P.F., Dos Santos, J.C., Hagen, F., Ribeiro-Dias, F., Queiroz-Telles, F., Netea, M.G., Meis, J.F., and Joosten, L.A.B.
- Abstract
Contains fulltext : 190812.pdf (publisher's version ) (Closed access), Cryptococcal species vary in capsule and cell size, thermotolerance, geographic distribution, and affected populations. Cryptococcus gattii sensu stricto and C. deuterogattii affect mainly immunocompetent hosts; however, C. bacillisporus, C. decagattii, and C. tetragattii cause infections mainly in immunocompromised hosts. This study aimed to compare the capacities of different species of the C. gattii species complex to induce cytokines and antimicrobial molecules in human peripheral blood mononuclear cells (PBMCs). Cryptococcus bacillisporus and C. deuterogattii induced the lowest levels of tumor necrosis factor alpha (TNF-alpha), interleukin-1beta (IL-1beta), and IL-6 among the five species of the C. gattii complex. Cryptococcus deuterogattii induced higher levels of IL-22 than those induced by C. tetragattii and the environmental species C. flavescens In addition, C. bacillisporus and C. gattii sensu stricto proliferated inside human monocyte-derived macrophages after 24 h of infection. All Cryptococcus species were able to generate reactive oxygen species (ROS) in human PBMCs, with C. bacillisporus and C. deuterogattii being more efficient than the other species. In conclusion, C. bacillisporus and C. deuterogattii induce lower levels of the proinflammatory cytokines TNF-alpha, IL-1beta, and IL-6 and higher ROS levels than those induced by the other species. Species of the Cryptococcus gattii complex have different abilities to induce cytokine and ROS production by human PBMCs.
- Published
- 2018
24. Differential In Vitro Cytokine Induction by the Species of Cryptococcus gattii Complex
- Author
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Herkert, P.F., Dos Santos, J.C., Hagen, F., Ribeiro-Dias, F., Queiroz-Telles, F., Netea, M.G., Meis, J.F., Joosten, L.A.B., Herkert, P.F., Dos Santos, J.C., Hagen, F., Ribeiro-Dias, F., Queiroz-Telles, F., Netea, M.G., Meis, J.F., and Joosten, L.A.B.
- Abstract
Contains fulltext : 190812.pdf (publisher's version ) (Closed access), Cryptococcal species vary in capsule and cell size, thermotolerance, geographic distribution, and affected populations. Cryptococcus gattii sensu stricto and C. deuterogattii affect mainly immunocompetent hosts; however, C. bacillisporus, C. decagattii, and C. tetragattii cause infections mainly in immunocompromised hosts. This study aimed to compare the capacities of different species of the C. gattii species complex to induce cytokines and antimicrobial molecules in human peripheral blood mononuclear cells (PBMCs). Cryptococcus bacillisporus and C. deuterogattii induced the lowest levels of tumor necrosis factor alpha (TNF-alpha), interleukin-1beta (IL-1beta), and IL-6 among the five species of the C. gattii complex. Cryptococcus deuterogattii induced higher levels of IL-22 than those induced by C. tetragattii and the environmental species C. flavescens In addition, C. bacillisporus and C. gattii sensu stricto proliferated inside human monocyte-derived macrophages after 24 h of infection. All Cryptococcus species were able to generate reactive oxygen species (ROS) in human PBMCs, with C. bacillisporus and C. deuterogattii being more efficient than the other species. In conclusion, C. bacillisporus and C. deuterogattii induce lower levels of the proinflammatory cytokines TNF-alpha, IL-1beta, and IL-6 and higher ROS levels than those induced by the other species. Species of the Cryptococcus gattii complex have different abilities to induce cytokine and ROS production by human PBMCs.
- Published
- 2018
25. IL-32gamma promotes the healing of murine cutaneous lesions caused by Leishmania braziliensis infection in contrast to Leishmania amazonensis
- Author
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Gomes, R.S., Silva, M.V.T., Santos, J.C. Dos, Silva, L.L. de Lima, Batista, A.C., Machado, J.R., Teixeira, M.M., Dorta, M.L., Oliveira, M.A. de, Dinarello, C.A., Joosten, L.A.B., Ribeiro-Dias, F., Gomes, R.S., Silva, M.V.T., Santos, J.C. Dos, Silva, L.L. de Lima, Batista, A.C., Machado, J.R., Teixeira, M.M., Dorta, M.L., Oliveira, M.A. de, Dinarello, C.A., Joosten, L.A.B., and Ribeiro-Dias, F.
- Abstract
Contains fulltext : 177364.pdf (publisher's version ) (Open Access), BACKGROUND: Interleukin 32 (IL-32) is a pro-inflammatory cytokine induced in patients with American tegumentary leishmaniasis (ATL) caused by Leishmania braziliensis. Here, we investigated whether IL-32 is also expressed in patient lesions caused by L. amazonensis. In addition, we evaluated experimental L. amazonensis and L. braziliensis infections in C57BL/6 transgenic mice for human IL-32gamma (IL-32gammaTg) in comparison with wild-type (WT) mice that do not express the IL-32 gene. RESULTS: Human cutaneous lesions caused by L. amazonensis express higher levels of IL-32 than healthy control skin. In mice, the presence of IL-32gamma promoted the control of cutaneous lesions caused by L. braziliensis, but not lesions caused by L. amazonensis in an ear dermis infection model. In addition, IL-32gammaTg mice displayed less tissue parasitism and inflammation in IL-32gammaTg than WT mice during the healing phase of L. braziliensis infection. Production of antigen-specific pro-inflammatory cytokines was higher in IL-32gammaTg mice than in WT mice during L. braziliensis infection but not during L. amazonensis infection. CONCLUSIONS: Human cutaneous lesions caused by L. amazonensis express high levels of IL-32. In mice, the presence of IL-32gamma contributes to the lesion healing caused by L. braziliensis but not by L. amazonensis. Data suggest that despite the ability for both species to induce IL-32 in humans, the connections between this cytokine and other immune players induced by related species of parasites can lead to distinct outcomes of the murine infections.
- Published
- 2017
26. Cytokines and microbicidal molecules regulated by IL-32 in THP-1-derived human macrophages infected with New World Leishmania species
- Author
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Santos, J.C. Dos, Heinhuis, B., Gomes, R.S., Damen, M.S.M.A., Real, F., Mortara, R.A., Keating, S.T., Dinarello, C.A., Joosten, L.A.B., Ribeiro-Dias, F., Santos, J.C. Dos, Heinhuis, B., Gomes, R.S., Damen, M.S.M.A., Real, F., Mortara, R.A., Keating, S.T., Dinarello, C.A., Joosten, L.A.B., and Ribeiro-Dias, F.
- Abstract
Contains fulltext : 169942.pdf (publisher's version ) (Open Access), BACKGROUND: Interleukin-32 (IL-32) is expressed in lesions of patients with American Tegumentary Leishmaniasis (ATL), but its precise role in the disease remains unknown. METHODOLOGY/PRINCIPAL FINDINGS: In the present study, silencing and overexpression of IL-32 was performed in THP-1-derived macrophages infected with Leishmania (Viannia) braziliensis or L. (Leishmania) amazonensis to investigate the role of IL-32 in infection. We report that Leishmania species induces IL-32gamma, and show that intracellular IL-32gamma protein production is dependent on endogenous TNFalpha. Silencing or overexpression of IL-32 demonstrated that this cytokine is closely related to TNFalpha and IL-8. Remarkably, the infection index was augmented in the absence of IL-32 and decreased in cells overexpressing this cytokine. Mechanistically, these effects can be explained by nitric oxide cathelicidin and beta-defensin 2 production regulated by IL-32. CONCLUSIONS: Thus, endogenous IL-32 is a crucial cytokine involved in the host defense against Leishmania parasites.
- Published
- 2017
27. The NOD2 receptor is crucial for immune responses towards New World Leishmania species
- Author
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Dos Santos, J.C., Damen, M.S.M.A., Oosting, M., Jong, D.J. de, Heinhuis, B., Gomes, R.S., Netea, M.G., Ribeiro-Dias, F., Joosten, L.A.B., Dos Santos, J.C., Damen, M.S.M.A., Oosting, M., Jong, D.J. de, Heinhuis, B., Gomes, R.S., Netea, M.G., Ribeiro-Dias, F., and Joosten, L.A.B.
- Abstract
Contains fulltext : 179623.pdf (publisher's version ) (Open Access)
- Published
- 2017
28. Interleukin 32: a novel player in the control of infectious diseases
- Author
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Ribeiro-Dias, F., Gomes, R., Silva, L.L. de Lima, Santos, J.C. Dos, Joosten, L.A.B., Ribeiro-Dias, F., Gomes, R., Silva, L.L. de Lima, Santos, J.C. Dos, and Joosten, L.A.B.
- Abstract
Contains fulltext : 169721.pdf (publisher's version ) (Closed access), Interleukin 32 (IL-32) is a proinflammatory cytokine, expressed as 9 distinct isoforms. The most active isoform is the predominantly intracellular-functioning IL-32gamma. Involvement of IL-32 in infectious diseases is increasingly being appreciated. Production of IL-32 promotes pathways that serve to control bacterial infection, especially those caused by mycobacteria. A similar role for this cytokine is observed in the cellular response to viral infections. In addition to its protective effects against microorganisms, IL-32 is involved in immunopathogenesis of some infectious diseases. In parasitic diseases, it has been demonstrated that this cytokine is induced by Leishmania infection. In this review, we summarize the present data on the role of IL-32 in infectious diseases, highlighting this cytokine as new target for control of infections.
- Published
- 2017
29. IL-32gamma promotes the healing of murine cutaneous lesions caused by Leishmania braziliensis infection in contrast to Leishmania amazonensis
- Author
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Gomes, R.S., Silva, M.V.T., Santos, J.C. Dos, Silva, L.L. de Lima, Batista, A.C., Machado, J.R., Teixeira, M.M., Dorta, M.L., Oliveira, M.A. de, Dinarello, C.A., Joosten, L.A.B., Ribeiro-Dias, F., Gomes, R.S., Silva, M.V.T., Santos, J.C. Dos, Silva, L.L. de Lima, Batista, A.C., Machado, J.R., Teixeira, M.M., Dorta, M.L., Oliveira, M.A. de, Dinarello, C.A., Joosten, L.A.B., and Ribeiro-Dias, F.
- Abstract
Contains fulltext : 177364.pdf (publisher's version ) (Open Access), BACKGROUND: Interleukin 32 (IL-32) is a pro-inflammatory cytokine induced in patients with American tegumentary leishmaniasis (ATL) caused by Leishmania braziliensis. Here, we investigated whether IL-32 is also expressed in patient lesions caused by L. amazonensis. In addition, we evaluated experimental L. amazonensis and L. braziliensis infections in C57BL/6 transgenic mice for human IL-32gamma (IL-32gammaTg) in comparison with wild-type (WT) mice that do not express the IL-32 gene. RESULTS: Human cutaneous lesions caused by L. amazonensis express higher levels of IL-32 than healthy control skin. In mice, the presence of IL-32gamma promoted the control of cutaneous lesions caused by L. braziliensis, but not lesions caused by L. amazonensis in an ear dermis infection model. In addition, IL-32gammaTg mice displayed less tissue parasitism and inflammation in IL-32gammaTg than WT mice during the healing phase of L. braziliensis infection. Production of antigen-specific pro-inflammatory cytokines was higher in IL-32gammaTg mice than in WT mice during L. braziliensis infection but not during L. amazonensis infection. CONCLUSIONS: Human cutaneous lesions caused by L. amazonensis express high levels of IL-32. In mice, the presence of IL-32gamma contributes to the lesion healing caused by L. braziliensis but not by L. amazonensis. Data suggest that despite the ability for both species to induce IL-32 in humans, the connections between this cytokine and other immune players induced by related species of parasites can lead to distinct outcomes of the murine infections.
- Published
- 2017
30. Cytokines and microbicidal molecules regulated by IL-32 in THP-1-derived human macrophages infected with New World Leishmania species
- Author
-
Santos, J.C. Dos, Heinhuis, B., Gomes, R.S., Damen, M.S.M.A., Real, F., Mortara, R.A., Keating, S.T., Dinarello, C.A., Joosten, L.A.B., Ribeiro-Dias, F., Santos, J.C. Dos, Heinhuis, B., Gomes, R.S., Damen, M.S.M.A., Real, F., Mortara, R.A., Keating, S.T., Dinarello, C.A., Joosten, L.A.B., and Ribeiro-Dias, F.
- Abstract
Contains fulltext : 169942.pdf (publisher's version ) (Open Access), BACKGROUND: Interleukin-32 (IL-32) is expressed in lesions of patients with American Tegumentary Leishmaniasis (ATL), but its precise role in the disease remains unknown. METHODOLOGY/PRINCIPAL FINDINGS: In the present study, silencing and overexpression of IL-32 was performed in THP-1-derived macrophages infected with Leishmania (Viannia) braziliensis or L. (Leishmania) amazonensis to investigate the role of IL-32 in infection. We report that Leishmania species induces IL-32gamma, and show that intracellular IL-32gamma protein production is dependent on endogenous TNFalpha. Silencing or overexpression of IL-32 demonstrated that this cytokine is closely related to TNFalpha and IL-8. Remarkably, the infection index was augmented in the absence of IL-32 and decreased in cells overexpressing this cytokine. Mechanistically, these effects can be explained by nitric oxide cathelicidin and beta-defensin 2 production regulated by IL-32. CONCLUSIONS: Thus, endogenous IL-32 is a crucial cytokine involved in the host defense against Leishmania parasites.
- Published
- 2017
31. The NOD2 receptor is crucial for immune responses towards New World Leishmania species
- Author
-
Dos Santos, J.C., Damen, M.S.M.A., Oosting, M., Jong, D.J. de, Heinhuis, B., Gomes, R.S., Netea, M.G., Ribeiro-Dias, F., Joosten, L.A.B., Dos Santos, J.C., Damen, M.S.M.A., Oosting, M., Jong, D.J. de, Heinhuis, B., Gomes, R.S., Netea, M.G., Ribeiro-Dias, F., and Joosten, L.A.B.
- Abstract
Contains fulltext : 179623.pdf (publisher's version ) (Open Access)
- Published
- 2017
32. Interleukin 32: a novel player in the control of infectious diseases
- Author
-
Ribeiro-Dias, F., Gomes, R., Silva, L.L. de Lima, Santos, J.C. Dos, Joosten, L.A.B., Ribeiro-Dias, F., Gomes, R., Silva, L.L. de Lima, Santos, J.C. Dos, and Joosten, L.A.B.
- Abstract
Contains fulltext : 169721.pdf (publisher's version ) (Closed access), Interleukin 32 (IL-32) is a proinflammatory cytokine, expressed as 9 distinct isoforms. The most active isoform is the predominantly intracellular-functioning IL-32gamma. Involvement of IL-32 in infectious diseases is increasingly being appreciated. Production of IL-32 promotes pathways that serve to control bacterial infection, especially those caused by mycobacteria. A similar role for this cytokine is observed in the cellular response to viral infections. In addition to its protective effects against microorganisms, IL-32 is involved in immunopathogenesis of some infectious diseases. In parasitic diseases, it has been demonstrated that this cytokine is induced by Leishmania infection. In this review, we summarize the present data on the role of IL-32 in infectious diseases, highlighting this cytokine as new target for control of infections.
- Published
- 2017
33. Interleukin 32: a novel player in the control of infectious diseases
- Author
-
Ribeiro-Dias, F., Gomes, R., Silva, L.L. de Lima, Santos, J.C. Dos, Joosten, L.A.B., Ribeiro-Dias, F., Gomes, R., Silva, L.L. de Lima, Santos, J.C. Dos, and Joosten, L.A.B.
- Abstract
Contains fulltext : 169721.pdf (publisher's version ) (Closed access), Interleukin 32 (IL-32) is a proinflammatory cytokine, expressed as 9 distinct isoforms. The most active isoform is the predominantly intracellular-functioning IL-32gamma. Involvement of IL-32 in infectious diseases is increasingly being appreciated. Production of IL-32 promotes pathways that serve to control bacterial infection, especially those caused by mycobacteria. A similar role for this cytokine is observed in the cellular response to viral infections. In addition to its protective effects against microorganisms, IL-32 is involved in immunopathogenesis of some infectious diseases. In parasitic diseases, it has been demonstrated that this cytokine is induced by Leishmania infection. In this review, we summarize the present data on the role of IL-32 in infectious diseases, highlighting this cytokine as new target for control of infections.
- Published
- 2017
34. The NOD2 receptor is crucial for immune responses towards New World Leishmania species
- Author
-
Dos Santos, J.C., Damen, M.S.M.A., Oosting, M., Jong, D.J. de, Heinhuis, B., Gomes, R.S., Netea, M.G., Ribeiro-Dias, F., Joosten, L.A.B., Dos Santos, J.C., Damen, M.S.M.A., Oosting, M., Jong, D.J. de, Heinhuis, B., Gomes, R.S., Netea, M.G., Ribeiro-Dias, F., and Joosten, L.A.B.
- Abstract
Contains fulltext : 179623.pdf (publisher's version ) (Open Access)
- Published
- 2017
35. IL-32gamma promotes the healing of murine cutaneous lesions caused by Leishmania braziliensis infection in contrast to Leishmania amazonensis
- Author
-
Gomes, R.S., Silva, M.V.T., Santos, J.C. Dos, Silva, L.L. de Lima, Batista, A.C., Machado, J.R., Teixeira, M.M., Dorta, M.L., Oliveira, M.A. de, Dinarello, C.A., Joosten, L.A.B., Ribeiro-Dias, F., Gomes, R.S., Silva, M.V.T., Santos, J.C. Dos, Silva, L.L. de Lima, Batista, A.C., Machado, J.R., Teixeira, M.M., Dorta, M.L., Oliveira, M.A. de, Dinarello, C.A., Joosten, L.A.B., and Ribeiro-Dias, F.
- Abstract
Contains fulltext : 177364.pdf (publisher's version ) (Open Access), BACKGROUND: Interleukin 32 (IL-32) is a pro-inflammatory cytokine induced in patients with American tegumentary leishmaniasis (ATL) caused by Leishmania braziliensis. Here, we investigated whether IL-32 is also expressed in patient lesions caused by L. amazonensis. In addition, we evaluated experimental L. amazonensis and L. braziliensis infections in C57BL/6 transgenic mice for human IL-32gamma (IL-32gammaTg) in comparison with wild-type (WT) mice that do not express the IL-32 gene. RESULTS: Human cutaneous lesions caused by L. amazonensis express higher levels of IL-32 than healthy control skin. In mice, the presence of IL-32gamma promoted the control of cutaneous lesions caused by L. braziliensis, but not lesions caused by L. amazonensis in an ear dermis infection model. In addition, IL-32gammaTg mice displayed less tissue parasitism and inflammation in IL-32gammaTg than WT mice during the healing phase of L. braziliensis infection. Production of antigen-specific pro-inflammatory cytokines was higher in IL-32gammaTg mice than in WT mice during L. braziliensis infection but not during L. amazonensis infection. CONCLUSIONS: Human cutaneous lesions caused by L. amazonensis express high levels of IL-32. In mice, the presence of IL-32gamma contributes to the lesion healing caused by L. braziliensis but not by L. amazonensis. Data suggest that despite the ability for both species to induce IL-32 in humans, the connections between this cytokine and other immune players induced by related species of parasites can lead to distinct outcomes of the murine infections.
- Published
- 2017
36. Interleukin 32: a novel player in the control of infectious diseases.
- Author
-
Ribeiro-Dias, F. and Ribeiro-Dias, F.
- Subjects
- Radboudumc 4: lnfectious Diseases and Global Health RIMLS: Radboud Institute for Molecular Life Sciences.
- Published
- 2017
37. Leishmania (Viannia) braziliensis amastigotes induces the expression of TNFalpha and IL-10 by human peripheral blood mononuclear cells in vitro in a TLR4-dependent manner
- Author
-
Galdino, H., Jr., Gomes, R., Santos, J.C. Dos, Pessoni, L.L., Maldaner, A.E., Marques, S.M., Gomes, C.M., Dorta, M.L., Oliveira, M.A. de, Joosten, L.A., Ribeiro-Dias, F., Galdino, H., Jr., Gomes, R., Santos, J.C. Dos, Pessoni, L.L., Maldaner, A.E., Marques, S.M., Gomes, C.M., Dorta, M.L., Oliveira, M.A. de, Joosten, L.A., and Ribeiro-Dias, F.
- Abstract
Contains fulltext : 170874.pdf (publisher's version ) (Closed access), While the role of Toll-like receptors (TLRs) has been investigated in murine models of tegumentary leishmaniasis caused by Leishmania (Viannia) braziliensis, the interaction between TLRs and Leishmania sp. has not been investigated in human cells. The aim of this study was to evaluate the involvement of TLR4 in cytokine production of human peripheral blood mononuclear cells (PBMCs) induced by L. braziliensis, and whether the parasite alters the expression of TLR4 on monocytes/macrophages. Amastigote forms were obtained from mice lesions and PBMCs were isolated from healthy donors. PBMCs were cultured in absence or presence of IFNgamma, TLR4 neutralizing antibodies, natural antagonist of TLR4 (Bartonella LPS), TLR4 agonist (E. coli LPS), and amastigote forms. The concentrations of tumor necrosis factor (TNFalpha) and interleukin 10 (IL-10) were assayed by ELISA and TLR4 expression by flow cytometry. Amastigotes forms of L. braziliensis induced TNFalpha and IL-10 production only in IFNgamma-primed PBMCs. The TNFalpha and IL-10 production was inhibited by TLR4 neutralization, both with anti-TLR4 antibodies and Bartonella LPS. Interestingly, addition of E. coli LPS further increased TNFalpha but not IL-10 production induced by L. braziliensis amastigotes. Amastigotes of L. braziliensis strongly reduced membrane TLR4 expression on monocytes/macrophages, apparently by internalization after the infection. The present study reveals that TLR4 drives the production of TNFalpha and IL-10 induced by L. braziliensis amastigotes and that the parasites decrease TLR4 expression on monocyte surface.
- Published
- 2016
38. Leishmania (Viannia) braziliensis amastigotes induces the expression of TNFalpha and IL-10 by human peripheral blood mononuclear cells in vitro in a TLR4-dependent manner
- Author
-
Galdino, H., Jr., Gomes, R., Santos, J.C. Dos, Pessoni, L.L., Maldaner, A.E., Marques, S.M., Gomes, C.M., Dorta, M.L., Oliveira, M.A. de, Joosten, L.A., Ribeiro-Dias, F., Galdino, H., Jr., Gomes, R., Santos, J.C. Dos, Pessoni, L.L., Maldaner, A.E., Marques, S.M., Gomes, C.M., Dorta, M.L., Oliveira, M.A. de, Joosten, L.A., and Ribeiro-Dias, F.
- Abstract
Contains fulltext : 170874.pdf (publisher's version ) (Closed access), While the role of Toll-like receptors (TLRs) has been investigated in murine models of tegumentary leishmaniasis caused by Leishmania (Viannia) braziliensis, the interaction between TLRs and Leishmania sp. has not been investigated in human cells. The aim of this study was to evaluate the involvement of TLR4 in cytokine production of human peripheral blood mononuclear cells (PBMCs) induced by L. braziliensis, and whether the parasite alters the expression of TLR4 on monocytes/macrophages. Amastigote forms were obtained from mice lesions and PBMCs were isolated from healthy donors. PBMCs were cultured in absence or presence of IFNgamma, TLR4 neutralizing antibodies, natural antagonist of TLR4 (Bartonella LPS), TLR4 agonist (E. coli LPS), and amastigote forms. The concentrations of tumor necrosis factor (TNFalpha) and interleukin 10 (IL-10) were assayed by ELISA and TLR4 expression by flow cytometry. Amastigotes forms of L. braziliensis induced TNFalpha and IL-10 production only in IFNgamma-primed PBMCs. The TNFalpha and IL-10 production was inhibited by TLR4 neutralization, both with anti-TLR4 antibodies and Bartonella LPS. Interestingly, addition of E. coli LPS further increased TNFalpha but not IL-10 production induced by L. braziliensis amastigotes. Amastigotes of L. braziliensis strongly reduced membrane TLR4 expression on monocytes/macrophages, apparently by internalization after the infection. The present study reveals that TLR4 drives the production of TNFalpha and IL-10 induced by L. braziliensis amastigotes and that the parasites decrease TLR4 expression on monocyte surface.
- Published
- 2016
39. Leishmania (Viannia) braziliensis amastigotes induces the expression of TNFalpha and IL-10 by human peripheral blood mononuclear cells in vitro in a TLR4-dependent manner
- Author
-
Galdino, H., Jr., Gomes, R., Santos, J.C. Dos, Pessoni, L.L., Maldaner, A.E., Marques, S.M., Gomes, C.M., Dorta, M.L., Oliveira, M.A. de, Joosten, L.A., Ribeiro-Dias, F., Galdino, H., Jr., Gomes, R., Santos, J.C. Dos, Pessoni, L.L., Maldaner, A.E., Marques, S.M., Gomes, C.M., Dorta, M.L., Oliveira, M.A. de, Joosten, L.A., and Ribeiro-Dias, F.
- Abstract
Contains fulltext : 170874.pdf (publisher's version ) (Closed access), While the role of Toll-like receptors (TLRs) has been investigated in murine models of tegumentary leishmaniasis caused by Leishmania (Viannia) braziliensis, the interaction between TLRs and Leishmania sp. has not been investigated in human cells. The aim of this study was to evaluate the involvement of TLR4 in cytokine production of human peripheral blood mononuclear cells (PBMCs) induced by L. braziliensis, and whether the parasite alters the expression of TLR4 on monocytes/macrophages. Amastigote forms were obtained from mice lesions and PBMCs were isolated from healthy donors. PBMCs were cultured in absence or presence of IFNgamma, TLR4 neutralizing antibodies, natural antagonist of TLR4 (Bartonella LPS), TLR4 agonist (E. coli LPS), and amastigote forms. The concentrations of tumor necrosis factor (TNFalpha) and interleukin 10 (IL-10) were assayed by ELISA and TLR4 expression by flow cytometry. Amastigotes forms of L. braziliensis induced TNFalpha and IL-10 production only in IFNgamma-primed PBMCs. The TNFalpha and IL-10 production was inhibited by TLR4 neutralization, both with anti-TLR4 antibodies and Bartonella LPS. Interestingly, addition of E. coli LPS further increased TNFalpha but not IL-10 production induced by L. braziliensis amastigotes. Amastigotes of L. braziliensis strongly reduced membrane TLR4 expression on monocytes/macrophages, apparently by internalization after the infection. The present study reveals that TLR4 drives the production of TNFalpha and IL-10 induced by L. braziliensis amastigotes and that the parasites decrease TLR4 expression on monocyte surface.
- Published
- 2016
40. Interleukin 32gamma (IL-32gamma) is highly expressed in cutaneous and mucosal lesions of American Tegumentary Leishmaniasis patients: association with tumor necrosis factor (TNF) and IL-10
- Author
-
Galdino, H., Jr., Maldaner, A.E., Pessoni, L.L., Soriani, F.M., Pereira, L.I., Pinto, S.A., Duarte, F.B., Gomes, C.M., Fleuri, A.K., Dorta, M.L., Oliveira, M.A. de, Teixeira, M.M., Batista, A.C., Joosten, L.A., Vieira, L.Q., Ribeiro-Dias, F., Galdino, H., Jr., Maldaner, A.E., Pessoni, L.L., Soriani, F.M., Pereira, L.I., Pinto, S.A., Duarte, F.B., Gomes, C.M., Fleuri, A.K., Dorta, M.L., Oliveira, M.A. de, Teixeira, M.M., Batista, A.C., Joosten, L.A., Vieira, L.Q., and Ribeiro-Dias, F.
- Abstract
Contains fulltext : 138334.pdf (publisher's version ) (Open Access), BACKGROUND: The interleukin 32 (IL-32) is a proinflammatory cytokine produced by immune and non-immune cells. It can be induced during bacterial and viral infections, but its production was never investigated in protozoan infections. American Tegumentary Leishmaniasis (ATL) is caused by Leishmania protozoan leading to cutaneous, nasal or oral lesions. The aim of this study was to evaluate the expression of IL-32 in cutaneous and mucosal lesions as well as in peripheral blood mononuclear cells (PBMC) exposed to Leishmania (Viannia) braziliensis. METHODS: IL-32, tumour necrosis factor (TNF) and IL-10 protein expression was evaluated by immunohistochemistry in cutaneous, mucosal lesions and compared to healthy specimens. The isoforms of IL-32alpha, beta, delta, gamma mRNA, TNF mRNA and IL-10 mRNA were assessed by qPCR in tissue biopsies of lesions and healthy skin and mucosa. In addition, PBMC from healthy donors were cultured with amastigotes of L. (V.) braziliensis. In lesions, the parasite subgenus was identified by PCR-RFLP. RESULTS: We showed that the mRNA expression of IL-32, in particular IL-32gamma was similarly up-regulated in lesions of cutaneous (CL) or mucosal (ML) leishmaniasis patients. IL-32 protein was produced by epithelial, endothelial, mononuclear cells and giant cells. The IL-32 protein expression was associated with TNF in ML but not in CL. IL-32 was not associated with IL-10 in both CL and ML. Expression of TNF mRNA was higher in ML than in CL lesions, however levels of IL-10 mRNA were similar in both clinical forms. In all lesions in which the parasite was detected, L. (Viannia) subgenus was identified. Interestingly, L. (V.) braziliensis induced only IL-32gamma mRNA expression in PBMC from healthy individuals. CONCLUSIONS: These data suggest that IL-32 plays a major role in the inflammatory process caused by L. (Viannia) sp or that IL-32 is crucial for controlling the L. (Viannia) sp infection.
- Published
- 2014
41. Interleukin 32gamma (IL-32gamma) is highly expressed in cutaneous and mucosal lesions of American Tegumentary Leishmaniasis patients: association with tumor necrosis factor (TNF) and IL-10
- Author
-
Galdino, H., Jr., Maldaner, A.E., Pessoni, L.L., Soriani, F.M., Pereira, L.I., Pinto, S.A., Duarte, F.B., Gomes, C.M., Fleuri, A.K., Dorta, M.L., Oliveira, M.A. de, Teixeira, M.M., Batista, A.C., Joosten, L.A., Vieira, L.Q., Ribeiro-Dias, F., Galdino, H., Jr., Maldaner, A.E., Pessoni, L.L., Soriani, F.M., Pereira, L.I., Pinto, S.A., Duarte, F.B., Gomes, C.M., Fleuri, A.K., Dorta, M.L., Oliveira, M.A. de, Teixeira, M.M., Batista, A.C., Joosten, L.A., Vieira, L.Q., and Ribeiro-Dias, F.
- Abstract
Contains fulltext : 138334.pdf (publisher's version ) (Open Access), BACKGROUND: The interleukin 32 (IL-32) is a proinflammatory cytokine produced by immune and non-immune cells. It can be induced during bacterial and viral infections, but its production was never investigated in protozoan infections. American Tegumentary Leishmaniasis (ATL) is caused by Leishmania protozoan leading to cutaneous, nasal or oral lesions. The aim of this study was to evaluate the expression of IL-32 in cutaneous and mucosal lesions as well as in peripheral blood mononuclear cells (PBMC) exposed to Leishmania (Viannia) braziliensis. METHODS: IL-32, tumour necrosis factor (TNF) and IL-10 protein expression was evaluated by immunohistochemistry in cutaneous, mucosal lesions and compared to healthy specimens. The isoforms of IL-32alpha, beta, delta, gamma mRNA, TNF mRNA and IL-10 mRNA were assessed by qPCR in tissue biopsies of lesions and healthy skin and mucosa. In addition, PBMC from healthy donors were cultured with amastigotes of L. (V.) braziliensis. In lesions, the parasite subgenus was identified by PCR-RFLP. RESULTS: We showed that the mRNA expression of IL-32, in particular IL-32gamma was similarly up-regulated in lesions of cutaneous (CL) or mucosal (ML) leishmaniasis patients. IL-32 protein was produced by epithelial, endothelial, mononuclear cells and giant cells. The IL-32 protein expression was associated with TNF in ML but not in CL. IL-32 was not associated with IL-10 in both CL and ML. Expression of TNF mRNA was higher in ML than in CL lesions, however levels of IL-10 mRNA were similar in both clinical forms. In all lesions in which the parasite was detected, L. (Viannia) subgenus was identified. Interestingly, L. (V.) braziliensis induced only IL-32gamma mRNA expression in PBMC from healthy individuals. CONCLUSIONS: These data suggest that IL-32 plays a major role in the inflammatory process caused by L. (Viannia) sp or that IL-32 is crucial for controlling the L. (Viannia) sp infection.
- Published
- 2014
42. Interleukin 32gamma (IL-32gamma) is highly expressed in cutaneous and mucosal lesions of American Tegumentary Leishmaniasis patients: association with tumor necrosis factor (TNF) and IL-10
- Author
-
Galdino, H., Jr., Maldaner, A.E., Pessoni, L.L., Soriani, F.M., Pereira, L.I., Pinto, S.A., Duarte, F.B., Gomes, C.M., Fleuri, A.K., Dorta, M.L., Oliveira, M.A. de, Teixeira, M.M., Batista, A.C., Joosten, L.A., Vieira, L.Q., Ribeiro-Dias, F., Galdino, H., Jr., Maldaner, A.E., Pessoni, L.L., Soriani, F.M., Pereira, L.I., Pinto, S.A., Duarte, F.B., Gomes, C.M., Fleuri, A.K., Dorta, M.L., Oliveira, M.A. de, Teixeira, M.M., Batista, A.C., Joosten, L.A., Vieira, L.Q., and Ribeiro-Dias, F.
- Abstract
Contains fulltext : 138334.pdf (publisher's version ) (Open Access), BACKGROUND: The interleukin 32 (IL-32) is a proinflammatory cytokine produced by immune and non-immune cells. It can be induced during bacterial and viral infections, but its production was never investigated in protozoan infections. American Tegumentary Leishmaniasis (ATL) is caused by Leishmania protozoan leading to cutaneous, nasal or oral lesions. The aim of this study was to evaluate the expression of IL-32 in cutaneous and mucosal lesions as well as in peripheral blood mononuclear cells (PBMC) exposed to Leishmania (Viannia) braziliensis. METHODS: IL-32, tumour necrosis factor (TNF) and IL-10 protein expression was evaluated by immunohistochemistry in cutaneous, mucosal lesions and compared to healthy specimens. The isoforms of IL-32alpha, beta, delta, gamma mRNA, TNF mRNA and IL-10 mRNA were assessed by qPCR in tissue biopsies of lesions and healthy skin and mucosa. In addition, PBMC from healthy donors were cultured with amastigotes of L. (V.) braziliensis. In lesions, the parasite subgenus was identified by PCR-RFLP. RESULTS: We showed that the mRNA expression of IL-32, in particular IL-32gamma was similarly up-regulated in lesions of cutaneous (CL) or mucosal (ML) leishmaniasis patients. IL-32 protein was produced by epithelial, endothelial, mononuclear cells and giant cells. The IL-32 protein expression was associated with TNF in ML but not in CL. IL-32 was not associated with IL-10 in both CL and ML. Expression of TNF mRNA was higher in ML than in CL lesions, however levels of IL-10 mRNA were similar in both clinical forms. In all lesions in which the parasite was detected, L. (Viannia) subgenus was identified. Interestingly, L. (V.) braziliensis induced only IL-32gamma mRNA expression in PBMC from healthy individuals. CONCLUSIONS: These data suggest that IL-32 plays a major role in the inflammatory process caused by L. (Viannia) sp or that IL-32 is crucial for controlling the L. (Viannia) sp infection.
- Published
- 2014
43. The molecular signature of oxidative metabolism and the mode of macrophage activation determine the shift from acute to chronic disease in experimental arthritis: critical role of interleukin-12p40.
- Author
-
Takahashi, N., Jager, V.C.L. de, Gluck, A., Letzkus, M., Hartmann, N., Staedtler, F., Ribeiro-Dias, F., Heuvelmans-Jacobs, M., Berg, W.B. van den, Joosten, L.A.B., Takahashi, N., Jager, V.C.L. de, Gluck, A., Letzkus, M., Hartmann, N., Staedtler, F., Ribeiro-Dias, F., Heuvelmans-Jacobs, M., Berg, W.B. van den, and Joosten, L.A.B.
- Abstract
Contains fulltext : 70716.pdf (publisher's version ) (Closed access), OBJECTIVE: Repeated injection of streptococcal cell wall (SCW) fragments results in chronic arthritis in mice. The objective of this study was to identify genes and pathways that determine disease progression based on gene expression profiling in this model. METHODS: Chronic arthritis was induced in mice by 4 injections of SCW fragments. RNA samples were isolated from synovial tissue obtained at various time points and were analyzed using mouse genome array and quantitative reverse transcription-polymerase chain reaction techniques. The functional role of potential key genes was evaluated in mice with specific gene deletions. RESULTS: Gene expression analyses revealed a shift in molecular signature. In contrast to an up-regulation of the inflammatory response pathway, the pathways involved in oxidative metabolism were significantly down-regulated during the chronic phase of arthritis. Since oxidative metabolism determines the mode of macrophage activation, we investigated phenotype switching in macrophages. Markers of alternatively activated macrophages, such as arginase 1, were at maximal levels during acute inflammation. In contrast, induction of markers of classically activated macrophages (M1), such as interleukin-1beta (IL-1beta) and inducible nitric oxide synthase (iNOS), was relatively low during the acute phase of disease, but highly increased toward the chronic phase. M1 polarization during the chronic phase was accompanied by a Th1 signature, characterized by IL-12p40, IL-12p35, and interferon-gamma. However, the absence of IL-12p40, but not IL-12p35, significantly inhibited the chronic phase of arthritis and was marked by a reduction in IL-17 and iNOS levels, as well as restored expression of oxidative metabolism genes. CONCLUSION: M1 polarization accompanied by a decline in oxidative metabolism determine the chronic phase of arthritis. IL-12p40, most likely acting through the IL-23/IL-17 axis, plays a critical role in this process.
- Published
- 2008
44. Stimulation of TLR2 and TLR4 differentially skews the balance of T cells in a mouse model of arthritis.
- Author
-
Abdollahi-Roodsaz, S., Joosten, L.A.B., Koenders, M.I., Devesa, I., Roelofs, M.F., Radstake, T.R.D.J., Heuvelmans-Jacobs, M., Akira, S., Nicklin, M.J., Ribeiro-Dias, F., Berg, W.B. van den, Abdollahi-Roodsaz, S., Joosten, L.A.B., Koenders, M.I., Devesa, I., Roelofs, M.F., Radstake, T.R.D.J., Heuvelmans-Jacobs, M., Akira, S., Nicklin, M.J., Ribeiro-Dias, F., and Berg, W.B. van den
- Abstract
Contains fulltext : 70944.pdf (publisher's version ) (Open Access), TLRs may contribute to the progression of rheumatoid arthritis through recognition of microbial or host-derived ligands found in arthritic joints. Here, we show that TLR2 and TLR4, but not TLR9, are involved in the pathogenesis of autoimmune arthritis and play distinct roles in the regulation of T cells and cytokines. We investigated the involvement of TLR2, TLR4, and TLR9 in the progression of arthritis using IL-1 receptor antagonist-knockout (IL1rn-/-) mice, which spontaneously develop an autoimmune T cell-mediated arthritis. Spontaneous onset of arthritis was dependent on TLR activation by microbial flora, as germ-free mice did not develop arthritis. Clinical and histopathological evaluation of IL1rn-/-Tlr2-/- mice revealed more severe arthritis, characterized by reduced suppressive function of Tregs and substantially increased IFN-gamma production by T cells. IL1rn-/-Tlr4-/- mice were, in contrast, protected against severe arthritis and had markedly lower numbers of Th17 cells and a reduced capacity to produce IL-17. A lack of Tlr9 did not affect the progression of arthritis. While any therapeutic intervention targeting TLR2 still seems complicated, the strict position of TLR4 upstream of a number of pathogenic cytokines including IL-17 provides an interesting potential therapeutic target for rheumatoid arthritis.
- Published
- 2008
45. The molecular signature of oxidative metabolism and the mode of macrophage activation determine the shift from acute to chronic disease in experimental arthritis: critical role of interleukin-12p40.
- Author
-
Takahashi, N., Jager, V.C.L. de, Gluck, A., Letzkus, M., Hartmann, N., Staedtler, F., Ribeiro-Dias, F., Heuvelmans-Jacobs, M., Berg, W.B. van den, Joosten, L.A.B., Takahashi, N., Jager, V.C.L. de, Gluck, A., Letzkus, M., Hartmann, N., Staedtler, F., Ribeiro-Dias, F., Heuvelmans-Jacobs, M., Berg, W.B. van den, and Joosten, L.A.B.
- Abstract
Contains fulltext : 70716.pdf (publisher's version ) (Closed access), OBJECTIVE: Repeated injection of streptococcal cell wall (SCW) fragments results in chronic arthritis in mice. The objective of this study was to identify genes and pathways that determine disease progression based on gene expression profiling in this model. METHODS: Chronic arthritis was induced in mice by 4 injections of SCW fragments. RNA samples were isolated from synovial tissue obtained at various time points and were analyzed using mouse genome array and quantitative reverse transcription-polymerase chain reaction techniques. The functional role of potential key genes was evaluated in mice with specific gene deletions. RESULTS: Gene expression analyses revealed a shift in molecular signature. In contrast to an up-regulation of the inflammatory response pathway, the pathways involved in oxidative metabolism were significantly down-regulated during the chronic phase of arthritis. Since oxidative metabolism determines the mode of macrophage activation, we investigated phenotype switching in macrophages. Markers of alternatively activated macrophages, such as arginase 1, were at maximal levels during acute inflammation. In contrast, induction of markers of classically activated macrophages (M1), such as interleukin-1beta (IL-1beta) and inducible nitric oxide synthase (iNOS), was relatively low during the acute phase of disease, but highly increased toward the chronic phase. M1 polarization during the chronic phase was accompanied by a Th1 signature, characterized by IL-12p40, IL-12p35, and interferon-gamma. However, the absence of IL-12p40, but not IL-12p35, significantly inhibited the chronic phase of arthritis and was marked by a reduction in IL-17 and iNOS levels, as well as restored expression of oxidative metabolism genes. CONCLUSION: M1 polarization accompanied by a decline in oxidative metabolism determine the chronic phase of arthritis. IL-12p40, most likely acting through the IL-23/IL-17 axis, plays a critical role in this process.
- Published
- 2008
46. Stimulation of TLR2 and TLR4 differentially skews the balance of T cells in a mouse model of arthritis.
- Author
-
Abdollahi-Roodsaz, S., Joosten, L.A.B., Koenders, M.I., Devesa, I., Roelofs, M.F., Radstake, T.R.D.J., Heuvelmans-Jacobs, M., Akira, S., Nicklin, M.J., Ribeiro-Dias, F., Berg, W.B. van den, Abdollahi-Roodsaz, S., Joosten, L.A.B., Koenders, M.I., Devesa, I., Roelofs, M.F., Radstake, T.R.D.J., Heuvelmans-Jacobs, M., Akira, S., Nicklin, M.J., Ribeiro-Dias, F., and Berg, W.B. van den
- Abstract
Contains fulltext : 70944.pdf (publisher's version ) (Open Access), TLRs may contribute to the progression of rheumatoid arthritis through recognition of microbial or host-derived ligands found in arthritic joints. Here, we show that TLR2 and TLR4, but not TLR9, are involved in the pathogenesis of autoimmune arthritis and play distinct roles in the regulation of T cells and cytokines. We investigated the involvement of TLR2, TLR4, and TLR9 in the progression of arthritis using IL-1 receptor antagonist-knockout (IL1rn-/-) mice, which spontaneously develop an autoimmune T cell-mediated arthritis. Spontaneous onset of arthritis was dependent on TLR activation by microbial flora, as germ-free mice did not develop arthritis. Clinical and histopathological evaluation of IL1rn-/-Tlr2-/- mice revealed more severe arthritis, characterized by reduced suppressive function of Tregs and substantially increased IFN-gamma production by T cells. IL1rn-/-Tlr4-/- mice were, in contrast, protected against severe arthritis and had markedly lower numbers of Th17 cells and a reduced capacity to produce IL-17. A lack of Tlr9 did not affect the progression of arthritis. While any therapeutic intervention targeting TLR2 still seems complicated, the strict position of TLR4 upstream of a number of pathogenic cytokines including IL-17 provides an interesting potential therapeutic target for rheumatoid arthritis.
- Published
- 2008
47. Stimulation of TLR2 and TLR4 differentially skews the balance of T cells in a mouse model of arthritis.
- Author
-
Abdollahi-Roodsaz, S., Joosten, L.A.B., Koenders, M.I., Devesa, I., Roelofs, M.F., Radstake, T.R.D.J., Heuvelmans-Jacobs, M., Akira, S., Nicklin, M.J., Ribeiro-Dias, F., Berg, W.B. van den, Abdollahi-Roodsaz, S., Joosten, L.A.B., Koenders, M.I., Devesa, I., Roelofs, M.F., Radstake, T.R.D.J., Heuvelmans-Jacobs, M., Akira, S., Nicklin, M.J., Ribeiro-Dias, F., and Berg, W.B. van den
- Abstract
Contains fulltext : 70944.pdf (publisher's version ) (Open Access), TLRs may contribute to the progression of rheumatoid arthritis through recognition of microbial or host-derived ligands found in arthritic joints. Here, we show that TLR2 and TLR4, but not TLR9, are involved in the pathogenesis of autoimmune arthritis and play distinct roles in the regulation of T cells and cytokines. We investigated the involvement of TLR2, TLR4, and TLR9 in the progression of arthritis using IL-1 receptor antagonist-knockout (IL1rn-/-) mice, which spontaneously develop an autoimmune T cell-mediated arthritis. Spontaneous onset of arthritis was dependent on TLR activation by microbial flora, as germ-free mice did not develop arthritis. Clinical and histopathological evaluation of IL1rn-/-Tlr2-/- mice revealed more severe arthritis, characterized by reduced suppressive function of Tregs and substantially increased IFN-gamma production by T cells. IL1rn-/-Tlr4-/- mice were, in contrast, protected against severe arthritis and had markedly lower numbers of Th17 cells and a reduced capacity to produce IL-17. A lack of Tlr9 did not affect the progression of arthritis. While any therapeutic intervention targeting TLR2 still seems complicated, the strict position of TLR4 upstream of a number of pathogenic cytokines including IL-17 provides an interesting potential therapeutic target for rheumatoid arthritis.
- Published
- 2008
48. The molecular signature of oxidative metabolism and the mode of macrophage activation determine the shift from acute to chronic disease in experimental arthritis: critical role of interleukin-12p40.
- Author
-
Takahashi, N., Jager, V.C.L. de, Gluck, A., Letzkus, M., Hartmann, N., Staedtler, F., Ribeiro-Dias, F., Heuvelmans-Jacobs, M., Berg, W.B. van den, Joosten, L.A.B., Takahashi, N., Jager, V.C.L. de, Gluck, A., Letzkus, M., Hartmann, N., Staedtler, F., Ribeiro-Dias, F., Heuvelmans-Jacobs, M., Berg, W.B. van den, and Joosten, L.A.B.
- Abstract
Contains fulltext : 70716.pdf (publisher's version ) (Closed access), OBJECTIVE: Repeated injection of streptococcal cell wall (SCW) fragments results in chronic arthritis in mice. The objective of this study was to identify genes and pathways that determine disease progression based on gene expression profiling in this model. METHODS: Chronic arthritis was induced in mice by 4 injections of SCW fragments. RNA samples were isolated from synovial tissue obtained at various time points and were analyzed using mouse genome array and quantitative reverse transcription-polymerase chain reaction techniques. The functional role of potential key genes was evaluated in mice with specific gene deletions. RESULTS: Gene expression analyses revealed a shift in molecular signature. In contrast to an up-regulation of the inflammatory response pathway, the pathways involved in oxidative metabolism were significantly down-regulated during the chronic phase of arthritis. Since oxidative metabolism determines the mode of macrophage activation, we investigated phenotype switching in macrophages. Markers of alternatively activated macrophages, such as arginase 1, were at maximal levels during acute inflammation. In contrast, induction of markers of classically activated macrophages (M1), such as interleukin-1beta (IL-1beta) and inducible nitric oxide synthase (iNOS), was relatively low during the acute phase of disease, but highly increased toward the chronic phase. M1 polarization during the chronic phase was accompanied by a Th1 signature, characterized by IL-12p40, IL-12p35, and interferon-gamma. However, the absence of IL-12p40, but not IL-12p35, significantly inhibited the chronic phase of arthritis and was marked by a reduction in IL-17 and iNOS levels, as well as restored expression of oxidative metabolism genes. CONCLUSION: M1 polarization accompanied by a decline in oxidative metabolism determine the chronic phase of arthritis. IL-12p40, most likely acting through the IL-23/IL-17 axis, plays a critical role in this process.
- Published
- 2008
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