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Bacteria were isolated seasonally from the Mediterranean sponges Chondrilla nucula and Petrosia ficiformis and screened for antibacterial activities. Selected isolates were taxonomically identified by 16S rRNA gene sequencing. A total of 416 different bacterial strains were isolated, 60 (14.4%) of which displayed variable degrees of antimicrobial activity. Of the bioactive strains, 58.3% were able to inhibit Staphylococcus aureus, 6.7% were active against Bacillus subtilis, 11.7% against both Enterococcus faecalis and Escherichia coli, 38.3% against Pseudoalteromonas atlantica and 33.3% against Pseudomonas elongata. 16S rRNA gene sequence analysis showed that 2 isolates, 1 from seawater samples and 1 from P. ficiformis, were most closely related to Bacillus subtilis (99% similarity) and that another isolate from P. ficiformis was most closely related to a previously described sponge-associated Alphaproteobacterium NW001 (98% similarity). Two isolates from C. nucula were most closely related to Brachybacterium paraconglomeratum (99% similarity) and Shewanella algae (89% similarity). The high percentage of bioactive isolates derived from the 2 sponges suggests that marine microorganisms, whether animal-associated or planktonic, are promising sources for drug discovery.

Bacteria were isolated seasonally from the Mediterranean sponges Chondrilla nucula and Petrosia ficiformis and screened for antibacterial activities. Selected isolates were taxonomically identified by 16S rRNA gene sequencing. A total of 416 different bacterial strains were isolated, 60 (14.4%) of which displayed variable degrees of antimicrobial activity. Of the bioactive strains, 58.3% were able to inhibit Staphylococcus aureus, 6.7% were active against Bacillus subtilis, 11.7% against both Enterococcus faecalis and Escherichia coli, 38.3% against Pseudoalteromonas atlantica and 33.3% against Pseudomonas elongata. 16S rRNA gene sequence analysis showed that 2 isolates, 1 from seawater samples and 1 from P. ficiformis, were most closely related to Bacillus subtilis (99% similarity) and that another isolate from P. ficiformis was most closely related to a previously described sponge-associated Alphaproteobacterium NW001 (98% similarity). Two isolates from C. nucula were most closely related to Brachybacterium paraconglomeratum (99% similarity) and Shewanella algae (89% similarity). The high percentage of bioactive isolates derived from the 2 sponges suggests that marine microorganisms, whether animal-associated or planktonic, are promising sources for drug discovery.