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The study has determined the influence of plasma-chemically activated water and its level of toxicity on test objects. It has been established that the reaction of biological objects is positive, which is reflected in their livelihood. The obtained research results show that the nature of the influence of water of different structures on the species composition of the selected test objects depends on the individual properties of each of them. The influence of plasma-chemically activated water on the functional properties of Riccia fluitans, Lémna mínor L., Paramecium сaudatum, Artemia salina and Cyprinus carpio has been investigated. The completed tests have helped supplement and substantiate the already existing idea about the properties of the plasma-chemically activated water and the possibilities of its use in the processing of raw materials and the manufacturing of finished products.In the course of the research, it was found that the Paramecium сaudatum survival was 87–89.5 %, with no toxic effects of the plasma-chemically activated water on these objects. The cultivation of Artemia salina in water that had been pre-treated by the proposed method showed that the number of viable organisms had increased by 60–70 % compared with the reference. The obtained results of studying the influence of the plasma-chemically activated water on the development of Riccia fluitans and Lémna mínor L. indicate that the activation of growth processes is 7–39 % higher than in the reference. It has been determined that the cultivation of Cyprinus carpio in the treated water contributes to increasing the survival rate as it improved the test objects by 13 % and 6 %, respectively, as to the reference. Using the method of gas-discharge diagnostics has shown that the treatment of water with nonthermal plasma for 30 minutes contributes to increasing its biopotential. Consequently, the obtained results indicate the expediency of using plasma-chemically activated water for the production, Досліджено вплив плазмохімічно активованої води на функціональний стан Riccia fluitans, Lémna mínor L., Paramecium сaudatum, Artemia salina і Cyprinus carpio. Визначено позитивну дію плазмохімічно активованої води на біологічні об’єкти. Встановлені основні показники для кожного методу біотестування. Отримані результати доповнюють відомості щодо безпечності використання плазмохімічно активованої води як компонента харчового ланцюга, Исследовано влияние плазмохимически активированной воды на функциональное состояние Riccia fluitans, Lémna mínor L., Paramecium сaudatum, Artemia salina и Cyprinus carpio. Определено позитивное действие плазмохимически активированной воды на биологические объекты. Установлены основные показатели для каждого метода биотестирования. Полученные результаты дополняют сведения о безопасности использования плазмохимически активированной воды как компонента пищевой цепи