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Interest in identifying pools of antibacterial-resistance genes has grown over the last decade, with veterinary antibiotics (VAs) receiving particular attention. In this paper, a mesoscale study aimed at evaluating the vertical transport of common VAs-namely, fluoroquinolones, tetracyclines, sulfonamides, and lincosamides in agricultural soil subjected to drip irrigation-was performed under greenhouse conditions. Accordingly, leachates of cropped and uncropped soil, amended with swine-slurry leading to 19-38 mu g kg(-1) (dry mass) antibiotics in the soil, were analyzed over the course of the productive cycle of a lettuce (42 days) with three sampling campaigns (N=24). High lincomycin (LCM) concentrations (30-39 mu g L-1) were detected in the leachates collected from the swine-slurry-amended soil. The highest LCM mass recovered in the leachates (30.1 +/- 1.63 %) was obtained from cropped experimental units. In addition, the LCM leaching constant and its leaching potential as obtained from the first-order model were higher in the leachates from the cropped experimental units. Lower concentrations of sulfadimethoxine were also detected in leachates and in soil. Enrofloxacin and oxytetracycline occurred only in soil, which is consistent with high soil interaction., Postprint (published version)

Interest in identifying pools of antibacterial-resistance genes has grown over the last decade, with veterinary antibiotics (VAs) receiving particular attention. In this paper, a mesoscale study aimed at evaluating the vertical transport of common VAs-namely, fluoroquinolones, tetracyclines, sulfonamides, and lincosamides in agricultural soil subjected to drip irrigation-was performed under greenhouse conditions. Accordingly, leachates of cropped and uncropped soil, amended with swine-slurry leading to 19-38 mu g kg(-1) (dry mass) antibiotics in the soil, were analyzed over the course of the productive cycle of a lettuce (42 days) with three sampling campaigns (N=24). High lincomycin (LCM) concentrations (30-39 mu g L-1) were detected in the leachates collected from the swine-slurry-amended soil. The highest LCM mass recovered in the leachates (30.1 +/- 1.63 %) was obtained from cropped experimental units. In addition, the LCM leaching constant and its leaching potential as obtained from the first-order model were higher in the leachates from the cropped experimental units. Lower concentrations of sulfadimethoxine were also detected in leachates and in soil. Enrofloxacin and oxytetracycline occurred only in soil, which is consistent with high soil interaction., Postprint (published version)

The capacity of a hybrid constructed wetland (CW) system consisting of two vertical flow (VF) CWs working alternatively (3 m(2)), one horizontal flow (HF) CW (2 m(2)) and one surface flow (FWS) CW (2 m(2)) in series to eliminate 13 emerging organic contaminants (EOCs) under three different hydraulic loading rates (HLRs) (0.06, 0.13 and 0.18 m d(-1) considering the area of the two VF beds) was studied through a continuous injection experiment General toxicity, dioxin-like activity, antimicrobial activity and estrogenicity were also measured under the highest hydraulic loading rate. The hybrid system was highly efficient on the removal of total injected EOCs (except for antibiotics, 43 +/- 32%) at all three HLRs (87 +/- 10%). The removal efficiency in the hybrid CW system showed to decrease as the HLR increased for most compounds. The VF wetlands removed most of the injected EOCs more efficiently than the other two CWs, which was attributable to the predominant aerobic degradation pathways of the VF beds (70 +/- 21%). General toxicity was reduced up to 90% by the VF beds. Estrogenicity and dioxin-like activity were similarly reduced by the VF and the HF wetlands, whereas antimicrobial activity was mainly removed by the FWS wetland. Bearing this in mind, this injection study has demonstrated that the use of hybrid CW systems is a suitable wastewater technology for removing EOCs and toxicity even at high HLRs. (C) 2013 Elsevier B.V. All rights reserved., Postprint (published version)

Interest in identifying pools of antibacterial-resistance genes has grown over the last decade, with veterinary antibiotics (VAs) receiving particular attention. In this paper, a mesoscale study aimed at evaluating the vertical transport of common VAs-namely, fluoroquinolones, tetracyclines, sulfonamides, and lincosamides in agricultural soil subjected to drip irrigation-was performed under greenhouse conditions. Accordingly, leachates of cropped and uncropped soil, amended with swine-slurry leading to 19-38 mu g kg(-1) (dry mass) antibiotics in the soil, were analyzed over the course of the productive cycle of a lettuce (42 days) with three sampling campaigns (N=24). High lincomycin (LCM) concentrations (30-39 mu g L-1) were detected in the leachates collected from the swine-slurry-amended soil. The highest LCM mass recovered in the leachates (30.1 +/- 1.63 %) was obtained from cropped experimental units. In addition, the LCM leaching constant and its leaching potential as obtained from the first-order model were higher in the leachates from the cropped experimental units. Lower concentrations of sulfadimethoxine were also detected in leachates and in soil. Enrofloxacin and oxytetracycline occurred only in soil, which is consistent with high soil interaction., Postprint (published version)