1. Design and Preparation of “corn-like” SPIONs@DFK-SBP-M13 Assembly for Improvement of Effective Internalization
- Author
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Zhang,Na, Wu,Hui, Liang,Yingzhi, Ye,Jianming, Zhang,Huan, Miao,Yuqing, Luo,Yane, Fan,Haiming, Yue,Tianli, Zhang,Na, Wu,Hui, Liang,Yingzhi, Ye,Jianming, Zhang,Huan, Miao,Yuqing, Luo,Yane, Fan,Haiming, and Yue,Tianli
- Abstract
Na Zhang,1,* Hui Wu,2,* Yingzhi Liang,1,* Jianming Ye,1 Huan Zhang,3 Yuqing Miao,3 Yane Luo,1 Haiming Fan,3 Tianli Yue1,4 1College of Food Science and Technology, Northwest University, Xiâan, Shaanxi, 710069, Peopleâs Republic of China; 2State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, Peopleâs Republic of China; 3Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xiâan, Shaanxi, 710069, Peopleâs Republic of China; 4Laboratory of Quality and Safety Risk Assessment for Agro-Products (Yangling), Ministry of Agriculture, Beijing, Peopleâs Republic of China*These authors contributed equally to this workCorrespondence: Yane Luo Email luoyane@nwu.edu.cnPurpose: Superparamagnetic iron oxide nanoparticles (SPIONs) have exhibited preeminent diagnosis and treatment performances, but their low internalization severely limits predesigned functions. The low cell internalization is now an urgent bottleneck problem for almost all nanomaterials. To achieve more internalization of SPIONS, recombinant M13 phage was designed for targeted delivery and smart release.Methods: M13 phages were designed to co-express exogenous SPARC binding peptide (SBP) and cathepsin B cleavage peptide (DFK), formed recombinant DFK-SBP-M13. 3.37± 0.06 nm of SPIONs were modified by 3, 4-dihydroxyhydrocinnamic acid (DHCA) to gain 10.80 ± 0.21 nm of DHCA-coated SPIONs, i.e., DHCA@SPIONs. Upon adjusting the proportions of DHCA@SPIONs and DFK-SBP-M13, the multi-carboxyl SPIONs assembled onto recombinant M13 phages via covalent bonding. The assemblies were co-cultured with MDA-MB-231 cells to interpret their internalization and smart release.Results: The âcorn-likeâ SPIONs@DFK-SBP-M13 (261.47± 3.30 nm) assemblies have not been reported previously. The assembly was stable, dispersible
- Published
- 2021