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This article examines two case studies related to LGBTQ disclosures of sexual violence on university campuses, in the US and Australia. We argue that in a landscape of mediated #MeToo discourse, LGBTQ testimonies are often mapped onto a cisheteropatriarchal framework which limits adequate institutional responses to LGBTQ experiences of sexual violence. We illustrate how resolutions are often imagined via individualised empowerment narratives, and how this works in concert with the cisheteropatriarchal framing to delimit responses to sexual violence. We consider alternative possibilities for accounting for LGBTQ experiences going forward, and how institutions like universities might better respond to these issues.

As COVID-19 began to unfold, I found myself confronted with a second predicament: an identity crisis. After nearly a decade of dating queer-identifying people entered a partnership with an ostensibly straight cisgender man. Up to this moment I had thought of myself as loosely affiliated with the label of “bisexual”, but I often used the term “queer” as a way to signal my disinterest in occupying normative space. I also (regrettably) felt that this term distanced myself from my socially disparaged bisexual kin. ii Yet, as a cisgender woman dating a cisgender man, a seemingly “normative” partnership was precisely the location I began to occupy at the beginning of 2020. The wider context of the pandemic forced a shrinking of worlds and a return to the domestic. Local lockdowns escalated a sense of urgency around clarifying intimate personal networks. As such, the crisis context forced to me confront my internal battle around identity and normativity in a shrunken and hyper-domestic sphere. I wondered, with my so-called “opposite sex” partner staying with me in lockdown, was I suddenly living a heteronormative life? Where does bisexuality fit in to this question of normative sexual formations and partnerships? More generally, I wondered to what extent interpersonal arrangements that provide safety, security and comfort in times of crisis always align with the “normative”.

This paper both revisits and productively draws on Judith Butler’s theory of sexuality offered in Gender Trouble, to consider how this foundational queer theory text inadvertently works to contain and bracket plurisexualities such as bisexuality. I consider how Butler at once rejects the idea of originary bisexuality provoked by psychoanalytic discourse on polymorphous perversity yet subsumes bisexual oppression into that of homosexuality. I interrogate Butler’s monosexual presumptions to extend their notion of the heterosexual matrix to account for an operational hetero/homo binary that both excludes plurisexuality as its Other and obscures this exclusion. Following this theoretical line, I consider how the asymmetric hetero/homo binary has been coded along the lines of comedy/tragedy in culture, and in contrast how bisexuality and other plurisexualities are often rendered impossible. I suggest that plurisexualities, representing a refusal to refuse the originary prohibition, “haunt” both straight and queer contexts alike.

While hair and beauty salons are often thought of as spaces that reproduce normative ideals of gender and sexuality, the past decade has seen an increase in salons catering specifically to LGBTQ+ clientele. More broadly, research has shown that far from only treating ‘surface’ concerns, salons in general are sites of intensified affect, making them a unique space of entangled body, emotional, and identity work. This article draws on interviews with salon workers from 2017 and client responses to a survey about salons during COVID-19 from 2020, conducted in Australia. This article gives particular focus to salons catering to the LGBTQ+ community (‘queer salons’), as well as data from salon clients that identify as LGBTQ+. Drawing on this dataset this article offers insight into how queer salons challenge our expectations of what hair and beauty salons can do for queer precarity in terms of physical, emotional and identity vulnerability and trouble ideas of the salon as a space which merely reinforces normative ideals of beauty. Furthermore, this article considers how salons might provide a sense of safety and belonging for some LGBTQ+ people, especially within the context of a pandemic.

This article reviews an assembled archive of the literature published to date on the sensory dimensions of the COVID-19 pandemic. Just as sensory scholars have often highlighted periods of sensory revolution, we find that the recent pandemic has augured some notable shifts, albeit often on the more micro and domestic scale. We present a five-sense sensorium that offers an overview of how the senses have been engaged with by scholars during the pandemic, and what the major issues and themes have been. Drawing on the literature, we suggest that there have been shifts in our sensate experiences and an increased awareness of the sensory dimensions of daily life that may usually go unnoticed. However, we also note the many sensory-related inequalities have been revealed over this period, which continue to unfold unevenly as the pandemic continues. We argue that going forward sensory scholars ought to attend to these questions of inequality, as well as tracking the possible undoing of some of the sensory revolutions that may have taken place so far.

During Australia’s first nationwide lockdown due to COVID-19 in 2020, hairdressers and barbers were allowed to remain operating while beauty salons and similar businesses were ordered to shut. This article offers some preliminary insights into the impact of the pandemic on salon workers during the period, in particular the additional emotional labour required. Drawing on a survey of salon workers based in Australia ( n = 92), this article considers the emotional labour involved in salon work in tandem with the impact of COVID-19 disruptions on this workforce. Results of the survey reveal the variety of emotional disclosures that salon workers generally encounter from clients and how these disclosures continued during the period, as well as the emotions experienced by workers themselves. Survey results suggest that many salon workers, who were themselves experiencing heightened levels of physical, emotional and financial vulnerability, were expected to continue their emotional roles for clients during a period of high anxiety and stress. This work suggests that future decision making ought to consider the impact on, and how best to support, all workforces who remain in operation during lockdowns, particularly emotional labourers, and not just those typically imagined as ‘essential’.

Community programs designed to train salon workers to address the issue of family violence are becoming increasingly commonplace. This article draws on interviews with trainees of one such program called HaiR-3Rs, run by the Eastern Domestic Violence Service (EDVOS) and launched in January 2018 in Victoria, Australia. HaiR-3Rs trains salon workers in recognising, responding to, and referring clients experiencing family violence. Using data collected from qualitative interviews this article reflects on trainee experiences of the HaiR-3Rs program. This article offers insights into whether training salon workers to respond to the issue of family violence places an additional burden of expectation on workers, as well as practical issues and limits of the training. The results of this study suggest that programs like HaiR-3Rs tap into deeper issues about the emotional nature of salon work, and has implications for the hair and beauty industry more broadly.

This article takes the documentary film The Hunting Ground, and the controversy it provoked, as a starting point for interrogating approaches to the representation and regulation of sexual assault on campus. We focus on the work of critical legal theorist Janet Halley, who has been a leading and contentious figure in advocating against the film and for a reconsideration of how Title IX is implemented on university campuses. In 2015, nineteen Harvard law professors, for whom Halley was the spokesperson, issued a press release objecting to the Hunting Ground for misrepresenting the case of one of their students.

In recent years there has been rising popular discourse around ‘toxic masculinity’, and the problems of a hegemonic gender structure that facilitates male violence and misogyny. In the public debate over whether toxic masculinity is fact or fiction, ‘toxic femininity’ is often raised by men’s rights activists and others as an anti-feminist retort, to suggest that women can be ‘toxic’ too. This paper provides a sketch of how the term has been used so far, in tandem with an overview of the limitations of the more extensively discussed idea of ‘toxic masculinity’. This paper suggests that rather than deploying ‘toxic femininity’, it is more useful to consider what might be ‘toxic’ about some approaches to femininity. Drawing on existing theories of femininity, including emphasised, hegemonic, normative, patriarchal femininity, pariah femininities, and femmephobia, this paper offers the notion of ‘rigid femininities’ to explain the structures that keep us locked into a ‘toxic’ gender system. This paper utilises the term toxic femininity as a jumping-off point for theorising femininity broadly. In theorising femininity, this paper offers a conceptualisation of the ‘toxic’ attachments that reinforce the gendered power structure/essentialized gender.

This article takes up references to breasts as a key case study to examine white Western feminist debate around embodiment and objectification. Tracking shifting understandings of ‘the gaze’ in these accounts, we find that objectification is often rendered singular, ahistorical and, increasingly, individually internalised. The history of these approaches to objectification helps to explain why during the early 2000s, theorisations of feminist politics-lost were often rhetorically located alongside discussions of surgically modified breasts as a symbol of a new era of ‘fake’ feminism. In contrast, the 2010s saw several feminist movements premised on exposure of flesh and claims to individual recuperation of bodily autonomy. This article contends that both of these perspectives rely on a notion, built over successive eras of white Western feminist thought, that political work can and ought to be done through the body as a site of representational politics. This article subsequently offers a brief insight into how we might queer our approach to breasts to better account for the messiness of experiences of the flesh, considering the personal as political, while not investing in the body as the site where politics must be enacted.

In this first-ever comprehensive examination of queerbaiting, fan studies scholar Joseph Brennan and his contributors examine cases that shed light on the sometimes exploitative industry practice of teasing homoerotic possibilities that, ...

Like other fangirls, fans of former boyband One Direction ("Directioners") have often been represented in media discourse as obsessive and hysterical, with fan behaviour interpreted as longing for heterosexual intimacy with band members. Subverting this heteronormative framing, a group of Directioners known as "Larries" have built a sub-fandom around imagining a relationship ("ship") between two of the band members, Harry Styles and Louis Tomlinson. Representation of the Larry fandom has gone beyond pathologizing fangirls to framing their shipping practice in terms of "fake news." The conspiracy theory panic around Larries misses the complex ways that subtext and queer reading are mobilized within the fandom to invoke feelings of queer intimacy and belonging. Drawing on a digital ethnography conducted on Twitter with Larries, we argue that these fans engage in queer reading strategies to explicitly imagine and interrupt dominant heterosexual narratives, and thus queer the figure of the fangirl.

In December 2017, Australia legalized same-sex marriage (SSM), following a 13-year ban and a drawn-out postal survey on marriage equality that saw campaigners mobilize for a ‘Yes’ vote on a non-binding poll. Through a discourse analysis of the Yes and No campaigns’ television and online video advertisements, we demonstrate how the Yes campaign was symptomatic of what we call a ‘post-liberation’ approach that saw SSM as the last major hurdle for Lesbian, Gay, Bisexual, Transgender, Intersex and Queer (LGBTIQ) politics. While the No campaign linked SSM to gender fluidity, transgender identity, and sex education programmes, in contrast the Yes campaign limited itself to narratives around love and marriage. In not attending to the link between sex, gender and sexuality, the Yes campaign narrowed the possibilities of the debate, preserving existing White heteronormative expectations of gender and sexuality. We contrast the debate that unfolded during the postal survey to the Australian Gay Liberation movement of the 1970s, the latter of which was able to successfully and radically challenge similarly homophobic campaigns. Rather than relying on ‘palatable’ or mainstream ideas of equality, love and fairness, Gay Liberation in Australia embraced the radical potential of LGBTIQ activism and presented a utopian, optimistic vision of a transformed future. Here we suggest that we can learn from the history of campaigns around sexuality, to understand what was ‘won’ in the SSM debate, and to better develop strategies for change in the future.

In December 2017, Australia legalized same-sex marriage (SSM), following a 13-year ban and a drawn-out postal survey on marriage equality that saw campaigners mobilize for a ‘Yes’ vote on a non-binding poll. Through a discourse analysis of the Yes and No campaigns’ television and online video advertisements, we demonstrate how the Yes campaign was symptomatic of what we call a ‘post-liberation’ approach that saw SSM as the last major hurdle for Lesbian, Gay, Bisexual, Transgender, Intersex and Queer (LGBTIQ) politics. While the No campaign linked SSM to gender fluidity, transgender identity, and sex education programmes, in contrast the Yes campaign limited itself to narratives around love and marriage. In not attending to the link between sex, gender and sexuality, the Yes campaign narrowed the possibilities of the debate, preserving existing White heteronormative expectations of gender and sexuality. We contrast the debate that unfolded during the postal survey to the Australian Gay Liberation movement of the 1970s, the latter of which was able to successfully and radically challenge similarly homophobic campaigns. Rather than relying on ‘palatable’ or mainstream ideas of equality, love and fairness, Gay Liberation in Australia embraced the radical potential of LGBTIQ activism and presented a utopian, optimistic vision of a transformed future. Here we suggest that we can learn from the history of campaigns around sexuality, to understand what was ‘won’ in the SSM debate, and to better develop strategies for change in the future.

The pathological sequestration of TAR DNA-binding protein 43 (TDP-43, encoded by TARDBP) into cytoplasmic pathological inclusions characterizes the distinct clinical syndromes of amyotrophic lateral sclerosis and behavioural variant frontotemporal dementia, while also co-occurring in a proportion of patients with Alzheimer's disease, suggesting that the regional concentration of TDP-43 pathology has most relevance to specific clinical phenotypes. This has been reflected in the three different pathological staging schemes for TDP-43 pathology in these different clinical syndromes, with none of these staging schemes including a preclinical phase similar to that which has proven beneficial in other neurodegenerative diseases. To apply each of these three staging schemes for TDP-43 pathology, the clinical phenotype must be known undermining the potential predictive value of the pathological examination. The present study set out to test whether a more unified approach could accurately predict clinical phenotypes based solely on the regional presence and severity of TDP-43 pathology. The selection of brain regions of interest was based on key regions routinely sampled for neuropathological assessment under current consensus criteria that have also been used in the three TDP-43 staging schemes. The severity of TDP-43 pathology in these regions of interest was assessed in four clinicopathological phenotypes: amyotrophic lateral sclerosis (n = 27, 47-78 years, 15 males), behavioural variant frontotemporal dementia (n = 15, 49-82 years, seven males), Alzheimer's disease (n = 26, 51-90 years, 11 males) and cognitively normal elderly individuals (n = 17, 80-103 years, nine males). Our results demonstrate that the presence of TDP-43 in the hypoglossal nucleus discriminates patients with amyotrophic lateral sclerosis with an accuracy of 98%. The severity of TDP-43 deposited in the anterior cingulate cortex identifies patients with behavioural variant frontotemporal dementia with

More than ever, our future depends upon how we manage the future of our waste. As an integrated part of sustainable development, effective waste management can reduce our global footprint. Ignoring or neglecting the challenges of waste, however, can lead to significant health, environmental and economic consequences. A staggering 1.3 billion tonnes of food is produced each year to feed the world’s 7 billion people. Yet, according to the Food and Agriculture Organization (FAO), around US$1 trillion of that food goes to waste. With 200,000 new people added every day, the world can ill afford to waste such a massive amount of food. Global waste, however, does not stop at food. Consumers are increasingly buying products that are wrapped in plastics and paper. Much of this packaging – and eventually the products themselves – will end up in landfills. This trend has both health and environmental consequences, especially given the rapid rise of hazardous waste such as electronics.

Background: We present a lipidomics analysis of human Parkinson’s disease tissues. We have focused on the primary visual cortex, a region that is devoid of pathological changes and Lewy bodies; and two additional regions, the amygdala and anterior cingulate cortex which contain Lewy bodies at different disease stages but do not have as severe degeneration as the substantia nigra. Methodology/Principal Findings: Using liquid chromatography mass spectrometry lipidomics techniques for an initial screen of 200 lipid species, significant changes in 79 sphingolipid, glycerophospholipid and cholesterol species were detected in the visual cortex of Parkinson’s disease patients (n = 10) compared to controls (n = 10) as assessed by two-sided unpaired t-test (p-value ,0.05). False discovery rate analysis confirmed that 73 of these 79 lipid species were significantly changed in the visual cortex (q-value ,0.05). By contrast, changes in 17 and 12 lipid species were identified in the Parkinson’s disease amygdala and anterior cingulate cortex, respectively, compared to controls; none of which remained significant after false discovery rate analysis. Using gas chromatography mass spectrometry techniques, 6 out of 7 oxysterols analysed from both non-enzymatic and enzymatic pathways were also selectively increased in the Parkinson’s disease visual cortex. Many of these changes in visual cortex lipids were correlated with relevant changes in the expression of genes involved in lipid metabolism and an oxidative stress response as determined by quantitative polymerase chain reaction techniques. Conclusions/Significance: The data indicate that changes in lipid metabolism occur in the Parkinson’s disease visual cortex in the absence of obvious pathology. This suggests that normalization of lipid metabolism and/or oxidative stress status in the visual cortex may represent a novel route for treatment of non-motor symptoms, such as visual hallucinations, that are experienced by a majority of

Background: We present a lipidomics analysis of human Parkinson’s disease tissues. We have focused on the primary visual cortex, a region that is devoid of pathological changes and Lewy bodies; and two additional regions, the amygdala and anterior cingulate cortex which contain Lewy bodies at different disease stages but do not have as severe degeneration as the substantia nigra. Methodology/Principal Findings: Using liquid chromatography mass spectrometry lipidomics techniques for an initial screen of 200 lipid species, significant changes in 79 sphingolipid, glycerophospholipid and cholesterol species were detected in the visual cortex of Parkinson’s disease patients (n = 10) compared to controls (n = 10) as assessed by two-sided unpaired t-test (p-value ,0.05). False discovery rate analysis confirmed that 73 of these 79 lipid species were significantly changed in the visual cortex (q-value ,0.05). By contrast, changes in 17 and 12 lipid species were identified in the Parkinson’s disease amygdala and anterior cingulate cortex, respectively, compared to controls; none of which remained significant after false discovery rate analysis. Using gas chromatography mass spectrometry techniques, 6 out of 7 oxysterols analysed from both non-enzymatic and enzymatic pathways were also selectively increased in the Parkinson’s disease visual cortex. Many of these changes in visual cortex lipids were correlated with relevant changes in the expression of genes involved in lipid metabolism and an oxidative stress response as determined by quantitative polymerase chain reaction techniques. Conclusions/Significance: The data indicate that changes in lipid metabolism occur in the Parkinson’s disease visual cortex in the absence of obvious pathology. This suggests that normalization of lipid metabolism and/or oxidative stress status in the visual cortex may represent a novel route for treatment of non-motor symptoms, such as visual hallucinations, that are experienced by a majority of

Background: We present a lipidomics analysis of human Parkinson’s disease tissues. We have focused on the primary visual cortex, a region that is devoid of pathological changes and Lewy bodies; and two additional regions, the amygdala and anterior cingulate cortex which contain Lewy bodies at different disease stages but do not have as severe degeneration as the substantia nigra. Methodology/Principal Findings: Using liquid chromatography mass spectrometry lipidomics techniques for an initial screen of 200 lipid species, significant changes in 79 sphingolipid, glycerophospholipid and cholesterol species were detected in the visual cortex of Parkinson’s disease patients (n = 10) compared to controls (n = 10) as assessed by two-sided unpaired t-test (p-value ,0.05). False discovery rate analysis confirmed that 73 of these 79 lipid species were significantly changed in the visual cortex (q-value ,0.05). By contrast, changes in 17 and 12 lipid species were identified in the Parkinson’s disease amygdala and anterior cingulate cortex, respectively, compared to controls; none of which remained significant after false discovery rate analysis. Using gas chromatography mass spectrometry techniques, 6 out of 7 oxysterols analysed from both non-enzymatic and enzymatic pathways were also selectively increased in the Parkinson’s disease visual cortex. Many of these changes in visual cortex lipids were correlated with relevant changes in the expression of genes involved in lipid metabolism and an oxidative stress response as determined by quantitative polymerase chain reaction techniques. Conclusions/Significance: The data indicate that changes in lipid metabolism occur in the Parkinson’s disease visual cortex in the absence of obvious pathology. This suggests that normalization of lipid metabolism and/or oxidative stress status in the visual cortex may represent a novel route for treatment of non-motor symptoms, such as visual hallucinations, that are experienced by a majority of

Background: We present a lipidomics analysis of human Parkinson’s disease tissues. We have focused on the primary visual cortex, a region that is devoid of pathological changes and Lewy bodies; and two additional regions, the amygdala and anterior cingulate cortex which contain Lewy bodies at different disease stages but do not have as severe degeneration as the substantia nigra. Methodology/Principal Findings: Using liquid chromatography mass spectrometry lipidomics techniques for an initial screen of 200 lipid species, significant changes in 79 sphingolipid, glycerophospholipid and cholesterol species were detected in the visual cortex of Parkinson’s disease patients (n = 10) compared to controls (n = 10) as assessed by two-sided unpaired t-test (p-value ,0.05). False discovery rate analysis confirmed that 73 of these 79 lipid species were significantly changed in the visual cortex (q-value ,0.05). By contrast, changes in 17 and 12 lipid species were identified in the Parkinson’s disease amygdala and anterior cingulate cortex, respectively, compared to controls; none of which remained significant after false discovery rate analysis. Using gas chromatography mass spectrometry techniques, 6 out of 7 oxysterols analysed from both non-enzymatic and enzymatic pathways were also selectively increased in the Parkinson’s disease visual cortex. Many of these changes in visual cortex lipids were correlated with relevant changes in the expression of genes involved in lipid metabolism and an oxidative stress response as determined by quantitative polymerase chain reaction techniques. Conclusions/Significance: The data indicate that changes in lipid metabolism occur in the Parkinson’s disease visual cortex in the absence of obvious pathology. This suggests that normalization of lipid metabolism and/or oxidative stress status in the visual cortex may represent a novel route for treatment of non-motor symptoms, such as visual hallucinations, that are experienced by a majority of

ATP-binding cassette transporter A1 (ABCA1) reduces amyloid-beta burden in transgenic mouse models of Alzheimer's disease (AD). Associations between ABCA1 polymorphisms and AD risk are also established. Little is known regarding the regulation of ABCA1 expression in the brain and how this may be affected by AD. In the present study we assessed ABCA1 mRNA and protein expression in the hippocampus of AD cases compared to controls. ABCA1 was clearly expressed in hippocampal neurons and expression was increased two-to three-fold in AD cases. The increased hippocampal ABCA1 expression was associated with increased APOE and PUMA gene expression, implying an association with neuronal stress. Consistent with this, treatment of SK-N-SH neurons with amyloid-beta peptide resulted in a 48% loss in survival and a significant upregulation of ABCA1, APOE, and PUMA gene expression. Studies in young (2 month) and old (12 month) transgenic mice expressing a familial AD form of human amyloid-beta protein precursor and presenilin-1 revealed a significant age-dependent upregulation of hippocampal Abca1 compared to wild-type control mice. However, hippocampal Apoe and Puma gene expression were not correlated with increased Abca1 expression in mice. Our data indicate that ABCA1 is upregulated in AD hippocampal neurons potentially via an amyloid-beta-mediated pathway.

ATP-binding cassette transporter A1 (ABCA1) reduces amyloid-beta burden in transgenic mouse models of Alzheimer's disease (AD). Associations between ABCA1 polymorphisms and AD risk are also established. Little is known regarding the regulation of ABCA1 expression in the brain and how this may be affected by AD. In the present study we assessed ABCA1 mRNA and protein expression in the hippocampus of AD cases compared to controls. ABCA1 was clearly expressed in hippocampal neurons and expression was increased two-to three-fold in AD cases. The increased hippocampal ABCA1 expression was associated with increased APOE and PUMA gene expression, implying an association with neuronal stress. Consistent with this, treatment of SK-N-SH neurons with amyloid-beta peptide resulted in a 48% loss in survival and a significant upregulation of ABCA1, APOE, and PUMA gene expression. Studies in young (2 month) and old (12 month) transgenic mice expressing a familial AD form of human amyloid-beta protein precursor and presenilin-1 revealed a significant age-dependent upregulation of hippocampal Abca1 compared to wild-type control mice. However, hippocampal Apoe and Puma gene expression were not correlated with increased Abca1 expression in mice. Our data indicate that ABCA1 is upregulated in AD hippocampal neurons potentially via an amyloid-beta-mediated pathway.

ATP-binding cassette transporter A1 (ABCA1) reduces amyloid-beta burden in transgenic mouse models of Alzheimer's disease (AD). Associations between ABCA1 polymorphisms and AD risk are also established. Little is known regarding the regulation of ABCA1 expression in the brain and how this may be affected by AD. In the present study we assessed ABCA1 mRNA and protein expression in the hippocampus of AD cases compared to controls. ABCA1 was clearly expressed in hippocampal neurons and expression was increased two-to three-fold in AD cases. The increased hippocampal ABCA1 expression was associated with increased APOE and PUMA gene expression, implying an association with neuronal stress. Consistent with this, treatment of SK-N-SH neurons with amyloid-beta peptide resulted in a 48% loss in survival and a significant upregulation of ABCA1, APOE, and PUMA gene expression. Studies in young (2 month) and old (12 month) transgenic mice expressing a familial AD form of human amyloid-beta protein precursor and presenilin-1 revealed a significant age-dependent upregulation of hippocampal Abca1 compared to wild-type control mice. However, hippocampal Apoe and Puma gene expression were not correlated with increased Abca1 expression in mice. Our data indicate that ABCA1 is upregulated in AD hippocampal neurons potentially via an amyloid-beta-mediated pathway.

ATP-binding cassette transporter A1 (ABCA1) reduces amyloid-beta burden in transgenic mouse models of Alzheimer's disease (AD). Associations between ABCA1 polymorphisms and AD risk are also established. Little is known regarding the regulation of ABCA1 expression in the brain and how this may be affected by AD. In the present study we assessed ABCA1 mRNA and protein expression in the hippocampus of AD cases compared to controls. ABCA1 was clearly expressed in hippocampal neurons and expression was increased two-to three-fold in AD cases. The increased hippocampal ABCA1 expression was associated with increased APOE and PUMA gene expression, implying an association with neuronal stress. Consistent with this, treatment of SK-N-SH neurons with amyloid-beta peptide resulted in a 48% loss in survival and a significant upregulation of ABCA1, APOE, and PUMA gene expression. Studies in young (2 month) and old (12 month) transgenic mice expressing a familial AD form of human amyloid-beta protein precursor and presenilin-1 revealed a significant age-dependent upregulation of hippocampal Abca1 compared to wild-type control mice. However, hippocampal Apoe and Puma gene expression were not correlated with increased Abca1 expression in mice. Our data indicate that ABCA1 is upregulated in AD hippocampal neurons potentially via an amyloid-beta-mediated pathway.

This paper draws on a digital ethnography conducted via Twitter, of a controversial subnetwork of fans of boyband One Direction known as “Larries”. The Larry fandom is built around imagining a romantic relationship exists between One Direction band members Harry Styles and Louis Tomlinson, referred to by the portmanteau “Larry”. Imagining relationships between celebrities is a controversial practice known in fan communities as “Real Person Slash” (RPS). For this practice Larries have been framed within popular media as conspiracy theorists. Our study sought to examine whether a more complex and nuanced understanding of the fandom was possible, particularly regarding relationships and community building in this fandom. Through digital ethnographic methods, we explored how the practices of Larries create a space in which new and unexpected desires, identities, intimacies and relations are constituted. Between March and July 2018, we followed approximately 490 public accounts, kept a fieldnote diary, and conducted semi-structured interviews with seven active users via direct messenger. Conducting observations on a daily basis over an extended period as regular fans would, we became privy to the highs, lows, excitement, and tensions of the group, that may be otherwise obscured. This study raises questions about what might be missed in studying controversial groups at a macro level only. This paper considers the benefits of undertaking qualitative digital ethnographic work and discuss the affective flows of digital networks on social media that might remain obscured by other digital methods.

n this paper, we review some numerical techniques based on the linear Krylov subspace iteration that can be used for the efficient calculation of the forward and the inverse electrical impedance tomography problems. Exploring their computational advantages in solving large-scale systems of equations, we specifically address their implementation in reconstructing localized impedance changes occurring within the human brain. If the conductivity of the head tissues is assumed to be real, the preconditioned conjugate gradients (PCGs) algorithm can be used to calculate efficiently the approximate forward solution to a given error tolerance. The performance and the regularizing properties of the PCG iteration for solving ill-conditioned systems of equations (PCGNs) is then explored, and a suitable preconditioning matrix is suggested in order to enhance its convergence rate. For image reconstruction, the nonlinear inverse problem is considered. Based on the Gauss-Newton method for solving nonlinear problems we have developed two algorithms that implement the PCGN iteration to calculate the linear step solution. Using an anatomically detailed model of the human head and a specific scalp electrode arrangement, images of a simulated impedance change inside brain's white matter have been reconstructed.

This document is part of the John Cocke papers that contains the personal, business, and legal papers of this 19th century Marengo County, Alabama, plantation owner, who not only managed his own plantation but also served as an agent for various family members. Financial papers consist of receipts from grocers and suppliers detailing purchases (including slave purchases); account books for his blacksmith shop; and labor accounts with payroll. There are cotton records that contain correspondence as well as accounts.

This document is part of the John Cocke papers that contains the personal, business, and legal papers of this 19th century Marengo County, Alabama, plantation owner, who not only managed his own plantation but also served as an agent for various family members. Financial papers consist of receipts from grocers and suppliers detailing purchases (including slave purchases); account books for his blacksmith shop; and labor accounts with payroll. There are cotton records that contain correspondence as well as accounts.