1. Electrochemical antibody-aptamer assay for VEGF cancer biomarker detection
- Author
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Ravalli, Andrea, Marrazza, Giovanna, Rivas, Lourdes, Escosura-Muñiz, Alfredo de la, Merkoçi, Arben, Ravalli, Andrea, Marrazza, Giovanna, Rivas, Lourdes, Escosura-Muñiz, Alfredo de la, and Merkoçi, Arben
- Abstract
In this work, a simple and sensitive approach for VEGF detection using antibody-aptamer assay and gold screen-printed electrodes as transducers is presented. The assay was performed in a sandwich format. Anti-VEGF antibody was first chemically immobilized on the gold working electrode surface of screen-printed cell. After the incubation with the antigen, the sandwich assay was realized by incubation step with biotinylated anti-VEGF aptamer. The sensor was then incubated with streptavidin-alkaline phosphatase and with 1-naphthyl phosphate. Differential pulse voltammetry (DPV) measurements were performed to detect VEGF biomarker.
- Published
- 2014