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The relative content of biologically active substances in the extracts of wood decaying fungi was studied using GC-MS. The results showed that the real tinder (Fomes fomentarius (L.) Fr.) contains up to 18% fatty acids, 51.6% steroids and 8.35% triterpene compounds. The nest fungus (Hapalopilus nidulans (Fr.) P. Karst.) contains 32.7% of fatty acids, 9.8% of steroid substances and 27.0% of tetracyclic triterpenoids. Chaga (Inonotus obliguus (Ach. ex. Pers.) Pilat) is characterized by the presence of 14% fatty acids, 11.6% of which are unsaturated; 2.4% of ergosterol, and 30% of betulin. A distinctive feature of the extract from the birch sponge (Piptoporus betulinus (Bull.) P. Karst.) is the presence of 67.9% arabitol, and the absence of steroid and triterpene compounds. Bordered tinder fungus (Fomitopsis pinicola (Sw.) P. Karst.), causing brown rot of wood, contains 30.15% ergosterol, which is a provitamin form of vitamin D2. The tinder fungus extract (Trichaptum biforme (Fr.) Ryvarden) contains 67.8% fatty acids, of which 50.0% are unsaturated fatty acids. The extract from the humpback tinder fungus (Trametes gibbosa (Pers.) Fr.) contains 34.9% fatty acids, 9.76% steroids, and 27.06% terpenoid compounds. © 2022 Author(s).

The optimal conditions for pretreatment protein substrates (mixture of cow’s milk whey proteins) and their hydrolysis by proteases of various classes for the target peptide fractions were determined. Were obtained by enzymatic hydrolysis of the whey protein of cow’s milk peptide fraction; qualitative and quantitative composition of the peptides were defined. Alcalase (a serine protease) in comparison with papain and neutrase (metal and cysteine protease) provides the most efficient hydrolysis of the major protein allergens whey: β -lactoglobulin, α-lactoalbumin. BSA removal is achieved by ultrafiltration or pre-heat treatment of the substrate. Optimum conditions for obtaining whey protein hydrolyzate is a mixture of pre-heat treatment of the substrates at 80°C for 10 min (pH 8.0), and subsequent cleavage of alkalas duration 120 min. At the same time there is a reduction in antigenicity of 20–25 times. It was established the absence in heat-treated hydr olysates bivalent antigenic determinants. Assessed the sensitizing effect of whey protein concentrate and milk enzymatic hydrolyzate.