1. Observation of Liquid-Liquid Phase Separation of FUS-LC using VUV-CD Spectroscopy
- Author
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Kentaro, Fujii, Nobuo, Maita, Masato, Kato, Koichi, Matsuo(Hiroshima Univ.), Kentaro, Fujii, Nobuo, Maita, Masato, Kato, and Koichi, Matsuo(Hiroshima Univ.)
- Abstract
Aggregation of the RNA-binding protein FUS (Fused in Sarcoma) has been implicated in the neurodegenerative diseases such as ALS (amyotrophic lateral sclerosis) and FTD (frontotemporal dementia) [1]. The low-complexity domain of the FUS (FUS-LC) mediated liquid-liquid phase separation (LLPS) [2], but the structural mechanism is not known in detail. To address the revealing the mechanism, several structural analyses such as NMR or x-ray crystallography were examined [2, 3]. Reentry, Murakami and co-authors were performed Raman microscopy to analyze LLPS local structure [4]. They revealed that the FUS LC have extremely high concentrations which could not achieved in vitro experiments. In order to reveal the process to form LLPS such a high concentration, we examined the spectroscopic study using VUV-CD measurement, which can analyze the secondary structure of the proteins., The 26th Hiroshima International Symposium on Synchrotron Radiation
- Published
- 2022