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Sreeranganathan M, Uthaman S, Sarmento B, et al. Int J Nanomedicine. 2017;12:7165–7182.   When reviewing the paper, the authors noted some errors in Figure 2E (page 7171) and Figure 6 (page 7176). They confirm that the corrections to Figures 2 and 6 do not alter the inferences or conclusion of the respective figures, nor the overall conclusion of the article, and apologize for this inadvertent error. In the original manuscript, Figure 2 represents the cellular internalization of the nanoparticles and Figure 2E represents the uptake of targeted nanoparticles by MKN-28 cells at a higher magnification. In Figure 2E, the fluorescence image was wrongly merged with the incorrect brightfield image of the cell. The authors have rectified this mistake by replacing Figure 2E with the confocal microscope image using the correct phase contrast, fluorescent, and merged images. The corrected version of Figure 2 is included as follows.   Read the original article &nbsp

Mohandas A, Kumar PT S, Raja B, et al. Int J Nanomedicine. 2015;10(Suppl 1):53–66. The authors have advised that Figure 8B and C (page 62) were incorrect in the original manuscript, as the Alginate control group is inadvertently repeated in the Alginate +0.1% nZnO group. Due to this mistake they have repeated the entire cell attachment studies using DAPI. The authors have confirmed that this does not in any way affect the conclusions from Figure 8B and C, and that it is not going to affect the overall conclusionof this published paper. They apologize for any inconvenience. The correct version of Figure 8 is presented below. Read the original article

Sreeranganathan M, Uthaman S, Sarmento B, et al. Int J Nanomedicine. 2017;12:7165–7182.   When reviewing the paper, the authors noted some errors in Figure 2E (page 7171) and Figure 6 (page 7176). They confirm that the corrections to Figures 2 and 6 do not alter the inferences or conclusion of the respective figures, nor the overall conclusion of the article, and apologize for this inadvertent error. In the original manuscript, Figure 2 represents the cellular internalization of the nanoparticles and Figure 2E represents the uptake of targeted nanoparticles by MKN-28 cells at a higher magnification. In Figure 2E, the fluorescence image was wrongly merged with the incorrect brightfield image of the cell. The authors have rectified this mistake by replacing Figure 2E with the confocal microscope image using the correct phase contrast, fluorescent, and merged images. The corrected version of Figure 2 is included as follows.   Read the original article &nbsp

Mohandas A, Kumar PT S, Raja B, et al. Int J Nanomedicine. 2015;10(Suppl 1):53–66. The authors have advised that Figure 8B and C (page 62) were incorrect in the original manuscript, as the Alginate control group is inadvertently repeated in the Alginate +0.1% nZnO group. Due to this mistake they have repeated the entire cell attachment studies using DAPI. The authors have confirmed that this does not in any way affect the conclusions from Figure 8B and C, and that it is not going to affect the overall conclusionof this published paper. They apologize for any inconvenience. The correct version of Figure 8 is presented below. Read the original article

Maya Sreeranganathan,1 Saji Uthaman,2 Bruno Sarmento,3–5 Chethampadi Gopi Mohan,1 In-Kyu Park,1 Rangasamy Jayakumar2 1Centre for Nanosciences and Molecular Medicine, Amrita University, Kochi, India; 2Department of Biomedical Science, BK21 PLUS Center for Creative Biomedical Scientists, Chonnam National University Medical School, Gwangju, Republic of Korea; 3I3S – Instituto de Investigação e Inovação em Saúde, University of Porto, Porto, Portugal, 4INEB – Instituto de Engenharia Biomédica, University of Porto, Porto, Portugal, 5CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Instituto Universitário de Ciências da Saúde, Gandra, Portugal Abstract: Epidermal growth factor receptor (EGFR), upregulated in gastric cancer patients, is an oncogene of interest in the development of targeted cancer nanomedicines. This study demonstrates in silico modeling of monoclonal antibody cetuximab (CET MAb)-conjugated docetaxel (DOCT)-loaded poly(γ-glutamic acid) (γ-PGA) nanoparticles (Nps) and evaluates the in vitro/in vivo effects on EGFR-overexpressing gastric cancer cells (MKN-28). Nontargeted DOCT-γ-PGA Nps (NT Nps: 110±40 nm) and targeted CET MAb-DOCT-γ-PGA Nps (T Nps: 200±20 nm) were prepared using ionic gelation followed by 1-Ethyl-3-(3-dimethyl aminopropyl)carbodiimide­– N-Hydoxysuccinimide (EDC­– NSH) chemistry. Increased uptake correlated with enhanced cytotoxicity induced by targeted Nps to EGFR +ve MKN-28 compared with nontargeted Nps as evident from MTT and flow cytometric assays. Nanoformulated DOCT showed a superior pharmacokinetic profile to that of free DOCT in Swiss albino mice, indicating the possibility of improved therapeutic e

Maya Sreeranganathan,1 Saji Uthaman,2 Bruno Sarmento,3–5 Chethampadi Gopi Mohan,1 In-Kyu Park,1 Rangasamy Jayakumar2 1Centre for Nanosciences and Molecular Medicine, Amrita University, Kochi, India; 2Department of Biomedical Science, BK21 PLUS Center for Creative Biomedical Scientists, Chonnam National University Medical School, Gwangju, Republic of Korea; 3I3S – Instituto de Investigação e Inovação em Saúde, University of Porto, Porto, Portugal, 4INEB – Instituto de Engenharia Biomédica, University of Porto, Porto, Portugal, 5CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Instituto Universitário de Ciências da Saúde, Gandra, Portugal Abstract: Epidermal growth factor receptor (EGFR), upregulated in gastric cancer patients, is an oncogene of interest in the development of targeted cancer nanomedicines. This study demonstrates in silico modeling of monoclonal antibody cetuximab (CET MAb)-conjugated docetaxel (DOCT)-loaded poly(γ-glutamic acid) (γ-PGA) nanoparticles (Nps) and evaluates the in vitro/in vivo effects on EGFR-overexpressing gastric cancer cells (MKN-28). Nontargeted DOCT-γ-PGA Nps (NT Nps: 110±40 nm) and targeted CET MAb-DOCT-γ-PGA Nps (T Nps: 200±20 nm) were prepared using ionic gelation followed by 1-Ethyl-3-(3-dimethyl aminopropyl)carbodiimide­– N-Hydoxysuccinimide (EDC­– NSH) chemistry. Increased uptake correlated with enhanced cytotoxicity induced by targeted Nps to EGFR +ve MKN-28 compared with nontargeted Nps as evident from MTT and flow cytometric assays. Nanoformulated DOCT showed a superior pharmacokinetic profile to that of free DOCT in Swiss albino mice, indicating the possibility of improved therapeutic e

Annapoorna Mohandas,* Sudheesh Kumar PT,* Biswas Raja, Vinoth-Kumar Lakshmanan, Rangasamy Jayakumar Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi, India *These authors contributed equally to this work Abstract: Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage was developed by freeze-dry method from the mixture of nZnO and alginate hydrogel. The developed composite bandage was porous with porosity at a range of 60%–70%. The swelling ratios of the bandages decreased with increasing concentrations of nZnO. The composite bandages with nZnO incorporation showed controlled degradation profile and faster blood clotting ability when compared to the KALTOSTAT® and control bandages without nZnO. The prepared composite bandages exhibited excellent antimicrobial activity against Escherichia coli, Staphylococcus aureus, Candida albicans, and methicillin resistant S. aureus (MRSA). Cytocompatibility evaluation of the prepared composite bandages done on human dermal fibroblast cells by Alamar assay and infiltration studies proved that the bandages have a non-toxic nature at lower concentrations of nZnO whereas slight reduction in viability was seen with increasing nZnO concentrations. The qualitative analysis of ex-vivo re-epithelialization on porcine skin revealed keratinocyte infiltration toward wound area for nZnO alginate bandages. Keywords: alginate, hydrogel, ZnO nanoparticle, hemostatic, antimicrobial activity, wound healingCorrigendum for this paper has been published    &nbsp

Annapoorna Mohandas,* Sudheesh Kumar PT,* Biswas Raja, Vinoth-Kumar Lakshmanan, Rangasamy Jayakumar Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi, India *These authors contributed equally to this work Abstract: Alginate hydrogel/zinc oxide nanoparticles (nZnO) composite bandage was developed by freeze-dry method from the mixture of nZnO and alginate hydrogel. The developed composite bandage was porous with porosity at a range of 60%–70%. The swelling ratios of the bandages decreased with increasing concentrations of nZnO. The composite bandages with nZnO incorporation showed controlled degradation profile and faster blood clotting ability when compared to the KALTOSTAT® and control bandages without nZnO. The prepared composite bandages exhibited excellent antimicrobial activity against Escherichia coli, Staphylococcus aureus, Candida albicans, and methicillin resistant S. aureus (MRSA). Cytocompatibility evaluation of the prepared composite bandages done on human dermal fibroblast cells by Alamar assay and infiltration studies proved that the bandages have a non-toxic nature at lower concentrations of nZnO whereas slight reduction in viability was seen with increasing nZnO concentrations. The qualitative analysis of ex-vivo re-epithelialization on porcine skin revealed keratinocyte infiltration toward wound area for nZnO alginate bandages. Keywords: alginate, hydrogel, ZnO nanoparticle, hemostatic, antimicrobial activity, wound healingCorrigendum for this paper has been published    &nbsp