1. Histological and histomorphometric studies on the cerebellar cortex and silver stained nucleolus organizer regions of Purkinje neurons in chronic morphine-treated rats
- Author
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Ilhami Celik, Muzaffer Seker, Ahmet Salbacak, Ilhami Celik, Muzaffer Seker, and Ahmet Salbacak
- Abstract
The effects of chronic morphine administration on the rat cerebellum and silver stained nucleolus organizer regions in Purkinje cells were investigated by means of histological, histochemical and histometrical techniques. Thirty-two young (30-32 days of age) Wistar rats (equal numbers of both genders) were randomly divided into 2 groups, as control and morphine-treated, each having equal numbers of both genders, a total of 16 animals. The control animals were injected subcutaneously with 1mL/kg physiological saline, and the morphine-treated rats received 5 mg/kg morphine hydrochloride subcutaneously at daily intervals for 30 days. The thickness of the molecular and granular layers of the cerebellum, the diameters of the nuclei and nucleoli of cerebellar Purkinje neurones, and the number and size of the silver staining nucleolus organizer regions of the Purkinje cell nuclei were determined histomorphometrically. The morphine administration caused slight histological changes in the cerebellum. The molecular layer thickness of the cerebellum was significantly (P<0.05) reduced, and the decrease was mainly in the male animals. In contrast, the layer thickened insignificantly in the morphine-treated females. The granular layer thickened slightly but insignificantly in both genders of the morphine-treated group. The Purkinje cell count significantly decreased with morphine treatment. Nucleus size did not change with morphine treatment. However, morphine-treated animals had smaller nucleoli. It was concluded that morphine treatment caused significant histomorphological changes in the cerebellar cortex in a sexually dimorphic manner., Učinci kronične primjene morfina na mali mozak štakora i na srebrom obojene regije nukleolusne organizacije Purkinjeovih neurona istraživani su histološkim, histokemijskim i histometrijskim tehnikama. Trideset i dva mlada Wistar štakora (dob 30 - 32 dana), u jednakom omjeru spolova, metodom slučajnog izbora podijeljena su u dvije skupine s po 16 jedinki u svakoj skupini. Životinjama u kontrolnoj skupini potkožno je primijenjen 1 mL/kg fiziološke otopine, dok su životinje u pokusnoj skupini dobile 5 mg/kg morfin hidroklorida, potkožno, svaki dan tijekom 30 dana. Za određivanje zadebljanja molekularnih i granulacijskih slojeva maloga mozga, promjera jezgre i nukleolusa Purkinjeovih neurona u malom mozgu te broja i veličine srebrom obojenih regija nukleolusne organizacije Purkinjeovih neurona primijenjena je histomorfometrijska metoda. Davanje morfina izazvalo je blage histološke promjene u malom mozgu. Debljina molekularnog sloja maloga mozga značajno je smanjena (P<0,05). To je ponajprije bilo izraženo kod životinja muškoga spola, dok je molekularni sloj kod ženki tretiranih morfinom bio tek neznatno zadebljan. Granulirani sloj bio je blago i nesignifikantno zadebljan u oba spola unutar skupine tretirane morfinom. Broj Purkinjeovih stanica značajno je smanjen nakon primjene morfina, dok to nije bio slučaj s veličinom jezgre koja se nije mijenjala. No, životinje koje su dobivale morfin imale su manje nukleole. Zaključeno je da liječenje morfinom uzrokuje značajne histomorfološke promjene u kori maloga mozga, pri čemu važnu ulogu ima spol jedinke.
- Published
- 2018