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1. Tetraselmis chuii Edible Microalga as a New Source of Neuroprotective Compounds Obtained Using Fast Biosolvent Extraction

Author

Cokdinleyen, Melis, Alvarez-Rivera, Gerardo, Tejera, Jose Luis González, Mendiola, José A., Valdés, Alberto, Kara, Huseyin, Ibáñez, Elena, Cifuentes, Alejandro, Cokdinleyen, Melis, Alvarez-Rivera, Gerardo, Tejera, Jose Luis González, Mendiola, José A., Valdés, Alberto, Kara, Huseyin, Ibáñez, Elena, and Cifuentes, Alejandro

Abstract

Tetraselmis chuii is an EFSA-approved novel food and dietary supplement with increasing use in nutraceutical production worldwide. This study investigated the neuroprotective potential of bioactive compounds extracted from T. chuii using green biobased solvents (ethyl acetate, AcOEt, and cyclopentyl methyl ether, CPME) under pressurized liquid extraction (PLE) conditions and supercritical fluid extraction (SFE). Response surface optimization was used to study the effect of temperature and solvent composition on the neuroprotective properties of the PLE extracts, including anticholinergic activity, reactive oxygen/nitrogen species (ROS/RNS) scavenging capacity, and anti-inflammatory activity. Optimized extraction conditions of 40 °C and 34.9% AcOEt in CPME resulted in extracts with high anticholinergic and ROS/RNS scavenging capacity, while operation at 180 °C and 54.1% AcOEt in CPME yielded extracts with potent anti-inflammatory properties using only 20 min. Chemical characterization revealed the presence of carotenoids (neoxanthin, violaxanthin, zeaxanthin, α- and β-carotene) known for their anti-cholinesterase, antioxidant, and anti-inflammatory potential. The extracts also exhibited high levels of omega-3 polyunsaturated fatty acids (PUFAs) with a favorable ω-3/ω-6 ratio (>7), contributing to their neuroprotective and anti-inflammatory effects. Furthermore, the extracts were found to be safe to use, as cytotoxicity assays showed no observed toxicity in HK-2 and THP-1 cell lines at or below a concentration of 40 μg mL−1. These results highlight the neuroprotective potential of Tetraselmis chuii extracts, making them valuable in the field of nutraceutical production and emphasize the interest of studying new green solvents as alternatives to conventional toxic solvents.

Published
2024

2. Hass avocado (Persea americana Mill.) residues as a new potential source of neuroprotective compounds using pressurized liquid extraction

Author

Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Consejo Superior de Investigaciones Científicas (España), Universidad Nacional de Colombia, Grisales-Mejía, Juan Felipe, Álvarez-Rivera, Gerardo, Torres-Castañeda, Harlen G., Andrade-Mahecha, Margarita M., Martínez-Correa, Hugo A., Mendiola, J. A., Cifuentes, Alejandro, Ibáñez, Elena, Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Consejo Superior de Investigaciones Científicas (España), Universidad Nacional de Colombia, Grisales-Mejía, Juan Felipe, Álvarez-Rivera, Gerardo, Torres-Castañeda, Harlen G., Andrade-Mahecha, Margarita M., Martínez-Correa, Hugo A., Mendiola, J. A., Cifuentes, Alejandro, and Ibáñez, Elena

Abstract

Epicarp and seed of Hass avocado (Persea americana Mill.) are agro-industrial residues rich in bioactive compounds. In this study, a pressurized liquid extraction (PLE) method was optimized to obtain phenolic compound-rich extracts with neuroprotective potential. The optimal extracts, obtained from 100% epicarp with pure water (0% ethanol) at 40 °C and 100 bar, demonstrated high extraction yield (26.8 ± 0.9%) and total phenol content (TPC = 505 ± 25 mg gallic acid equivalent/g dry extract), good antioxidant capacity (ABTS = 3350 ± 179 µmol Trolox equivalent/g dry extract, ORAC = 0.14 ± 0.01 µg/mL), and moderate acetylcholinesterase inhibition (33.6 ± 2.9 µg/mL). UHPLC-ESI-QTOF-MS analysis of the optimal extracts tentatively identified phenolic acids, phenolic alcohol derivatives, and various flavonoids; namely flavonols. The results reveal the potential of avocado processing by-products, particularly the epicarp, as source of potential neuroprotective agents. The proposed PLE methodology was demonstrated to be a sustainable and efficient strategy for avocado residues valorization.

Published
2024

3. Optimization of supercritical fluid extraction of bioactive compounds from Ammodaucus leucotrichus fruits by using multivariate response surface methodology

Author

Fundação de Amparo à Pesquisa do Estado de São Paulo, Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Consejo Superior de Investigaciones Científicas (España), Abderrezag, Norelhouda, Sanchez Bragagnolo, Felipe, Louaer, Ouahida, Meniai, Abdeslam-Hassen, Cifuentes, Alejandro, Ibáñez, Elena, Mendiola, J. A., Fundação de Amparo à Pesquisa do Estado de São Paulo, Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Consejo Superior de Investigaciones Científicas (España), Abderrezag, Norelhouda, Sanchez Bragagnolo, Felipe, Louaer, Ouahida, Meniai, Abdeslam-Hassen, Cifuentes, Alejandro, Ibáñez, Elena, and Mendiola, J. A.

Abstract

This study explored supercritical fluid extraction (SFE) of bioactive molecules from Ammodaucus leucotrichus seeds, a little-known source of potent bioactive compounds. Response surface methodology assessed extraction parameters (pressure, temperature, and ethanol co-solvent) and their effects on global yield, total phenolic compound (TPC), total carbohydrate content (TCC), and anti-Alzheimer activity. Optimization via a Box-Behnken design identified the optimal conditions: 15 % ethanol, 300 bar, and 70 °C. Chemical profiling using GC-MS and UHPLC-q-tof-MS/MS detected over 41 metabolites. In addition, a biorefinery sequential extraction process was performed to obtain extracts with high neuroprotective potential. The SFE extracts exhibited modest AChE inhibition. Notably, sequential SFE and PLE extractions yielded extracts with enhanced antioxidant activity compared to separate processes. In conclusion, Ammodaucus leucotrichus presents a promising natural source of secondary metabolites. Its potential health benefits warrant further exploration, emphasizing the need for continued research.

Published
2024

4. Pressurized liquid extraction of glucosinolates from Camelina sativa (L.) Crantz by-products: Process optimization and biological activities of green extract

Author

European Commission, Ministero dell'Istruzione, dell'Università e della Ricerca, Pagliari, Stefania, Domínguez-Rodríguez, Gloria, Cifuentes, Alejandro, Ibáñez, Elena, Labra, Massimo, Campone, Luca, European Commission, Ministero dell'Istruzione, dell'Università e della Ricerca, Pagliari, Stefania, Domínguez-Rodríguez, Gloria, Cifuentes, Alejandro, Ibáñez, Elena, Labra, Massimo, and Campone, Luca

Abstract

The cultivation of Camelina sativa (L.) Crantz is rapidly increasing due to oil production resulting in a substantial volume of by-products, which still have an interesting composition in secondary metabolites, especially glucosinolates. Therefore, a green extraction procedure of glucosinolates by Pressurised Liquid Extraction was developed and optimized using a chemometric approach. Furthermore, the glucosinolates were purified by solid phase extraction, and a preliminary study on bioaccessibility and bioavailability study was carried out to evaluate the resistance of the glucosinolates to the digestive process. The application of pressurised liquid extraction to the recovery of glucosinolates from camelina sativa by-product, is a green, automatic, and rapid method, representing a valid alternative to conventional extraction method to obtain ingredients for food industries.

Published
2024

5. A Prunus avium L. infusion inhibits sugar uptake and counteracts oxidative stress-induced stimulation of glucose uptake by intestinal epithelial (Caco-2) cells

Author

Fundação para a Ciência e a Tecnologia (Portugal), Ministério da Ciência, Tecnologia e Ensino Superior (Portugal), European Commission, Barreto Peixoto, Juliana A., Silva, Cláudia, Costa, Anabela S. G., Álvarez-Rivera, Gerardo, Cifuentes, Alejandro, Ibáñez, Elena, Oliveira, M. Beatriz P. P., Alves, Rita C., Martel, Fátima, Andrade, Nelson, Fundação para a Ciência e a Tecnologia (Portugal), Ministério da Ciência, Tecnologia e Ensino Superior (Portugal), European Commission, Barreto Peixoto, Juliana A., Silva, Cláudia, Costa, Anabela S. G., Álvarez-Rivera, Gerardo, Cifuentes, Alejandro, Ibáñez, Elena, Oliveira, M. Beatriz P. P., Alves, Rita C., Martel, Fátima, and Andrade, Nelson

Abstract

Sweet cherry (Prunus avium L.) is among the most valued fruits due to its organoleptic properties and nutritional worth. Cherry stems are rich in bioactive compounds, known for their anti-inflammatory and antioxidant properties. Innumerable studies have indicated that some bioactive compounds can modulate sugar absorption in the small intestine. In this study, the phenolic profile of a cherry stem infusion was investigated, as well as its capacity to modulate intestinal glucose and fructose transport in Caco-2 cells. Long-term (24 h) exposure to cherry stem infusion (25%, v/v) significantly reduced glucose (3H-DG) and fructose (14C-FRU) apical uptake, reduced the apical-to-basolateral Papp to 3H-DG, and decreased mRNA expression levels of the sugar transporters SGLT1, GLUT2 and GLUT5. Oxidative stress (induced by tert-butyl hydroperoxide) caused an increase in 3H-DG uptake, which was abolished by the cherry stem infusion. These findings suggest that cherry stem infusion can reduce the intestinal absorption of both glucose and fructose by decreasing the gene expression of their membrane transporters. Moreover, this infusion also appears to be able to counteract the stimulatory effect of oxidative stress upon glucose intestinal uptake. Therefore, it can be a potentially useful compound for controlling hyperglycemia, especially in the presence of increased intestinal oxidative stress levels.

Published
2024

6. Chemical profile of cell cultures of Kalanchoë gastonis-bonnieri transformed by Agrobacterium rhizogenes

Author

Consejo Nacional de Ciencia y Tecnología (México), Instituto Politécnico Nacional (México), Barrera Núñez, María Guadalupe, Bueno, Mónica, Molina-Montiel, Miguel Ángel, Reyes-Vaquero, Lorena, Ibáñez, Elena, Del Villar-Martínez, Alma, Consejo Nacional de Ciencia y Tecnología (México), Instituto Politécnico Nacional (México), Barrera Núñez, María Guadalupe, Bueno, Mónica, Molina-Montiel, Miguel Ángel, Reyes-Vaquero, Lorena, Ibáñez, Elena, and Del Villar-Martínez, Alma

Abstract

Kalanchoë gastonis-bonnieri Raym.-Hamet & Perrier is a plant used for medicinal purposes in the treatment of several ailments. The aim of this study was to analyze the chemical profile of extracts from K. gastonis-bonnieri embryogenic calli, generated from genetically transformed roots by Agrobacterium rhizogenes. Putative transformants were verified by PCR. Hydroalcoholic extracts were obtained and the chemical profile was analyzed by LC-ESI-MS/MS. Root formation was obtained from 80% of infected seedlings. Fifteen root lines were isolated, and two lines showed prominent longitudinal growth and profuse branching in the B5 semi-solid medium. In all lines, the formation of nodules and later embryogenic callus was observed. Putative transgenic root lines were cultivated in free-plant growth regulators B5 medium. In the two selected lines, the PCR amplification of rolA, rolB, rolC, rolD, and aux1 genes was detected. The extract of embryogenic calli showed 60 chemical compounds tentatively identified, such as ferulic acid, quinic acid, neobaisoflavone, and malic acid, among others, and the chemical profile was different in comparison to wild-type extracts. This is the first study reporting the analysis of the chemical profile of hairy root extracts derived from Kalanchoë gastonis-bonnieri. This work displays the great potential for obtaining chemical compounds of pharmacological importance from hairy roots and facilitates the identification of new useful drugs against human chronic-degenerative diseases.

Published
2024

7. An eco-friendly extraction method to obtain pectin from passion fruit rinds (Passiflora edulis sp.) using subcritical water and pressurized natural deep eutectic solvents

Author

Sao Paulo Research Foundation, Fundação de Amparo à Pesquisa do Estado de São Paulo, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia e Innovación (España), Pereira, Débora Tamires Vitor, Méndez-Albiñana, Pablo, Mendiola, J. A., Villamiel, Mar, Cifuentes, Alejandro, Martínez, Julian, Ibáñez, Elena, Sao Paulo Research Foundation, Fundação de Amparo à Pesquisa do Estado de São Paulo, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia e Innovación (España), Pereira, Débora Tamires Vitor, Méndez-Albiñana, Pablo, Mendiola, J. A., Villamiel, Mar, Cifuentes, Alejandro, Martínez, Julian, and Ibáñez, Elena

Abstract

This work evaluated the efficiency of Subcritical Water Extraction (SWE) and Pressurized Natural Deep Eutectic Solvents (P-NaDESs) under different temperatures (100, 120, 140 and 160 °C) in obtaining pectin from Passion Fruit Rinds (PFR) and its residual biomass (PFR - UAPLE), and compare the results with those of Conventional Extraction (CE). The highest pectin yields, 19.1 and 27.6 %, were achieved using P-NaDES (Citric Acid:Glucose:Water) at 120 °C for PFR and its PFR-UAPLE, respectively. Regarding the Degree of Esterification (DE), pectin obtained with SWE and CE had DE below 50 %, while with P-NaDES (Citric Acid: Glucose:Water), DE was above 50 %. Higher Molecular Weights (MW) (98 and 81 kDa) were obtained with SWE and P-NaDES from PFR compared to PFR-UAPLE and CE. Galacturonic acid was the most abundant (74 to 78 %) monosaccharide obtained by SWE. In terms of morphology, water extraction provided pectin with more uniform textures, whereas extraction with acidified mixtures led to more heterogeneous surfaces. Overall, comparing SWE and P-NaDES, the obtained pectins differed in terms of monomeric composition, MW and DE. These results indicate that pectins obtained by both methods can have different applications depending on their structural characteristics.

Published
2024

8. A comprehensive study on the chemical characterization and neuroprotective evaluation of pracaxi nuts extracts obtained by a sustainable approach

Author

Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Agencia Nacional de Investigación y Desarrollo (Chile), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Mohammadnezhad, Pouya [0009-0004-8838-3225], Valdés, Alberto [0000-0002-7901-5816], Barrientos, Ruth E. [0000-0002-7162-9290], Ibáñez, Elena [0000-0003-2127-8303], Cifuentes, Alejandro [0000-0002-7464-0217], Mohammadnezhad, Pouya, Valdés, Alberto, Barrientos, Ruth E., Ibáñez, Elena, Block, Jane Mara, Cifuentes, Alejandro, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Agencia Nacional de Investigación y Desarrollo (Chile), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Mohammadnezhad, Pouya [0009-0004-8838-3225], Valdés, Alberto [0000-0002-7901-5816], Barrientos, Ruth E. [0000-0002-7162-9290], Ibáñez, Elena [0000-0003-2127-8303], Cifuentes, Alejandro [0000-0002-7464-0217], Mohammadnezhad, Pouya, Valdés, Alberto, Barrientos, Ruth E., Ibáñez, Elena, Block, Jane Mara, and Cifuentes, Alejandro

Abstract

The Amazonian Pentaclethra macroloba (Willd.) Kuntze nuts contain a lipidic fraction with health-promoting effects, but little is known about the bioactivity of other constituents. In this study, the lipidic fraction obtained using supercritical fluid extraction (SFE) with CO2 was chemically characterized by using lipidomics techniques. The SFE-CO2 residue, named as pracaxi cake, was re-extracted by pressurized liquid extraction following a biorefinery approach. Using a response surface methodology and based on the extraction yield and different in vitro assays, two optimum conditions were obtained: 80% and 12.5% of ethanol at 180 °C. Under these conditions, extraction yield and different in vitro measurements related to neuroprotection were assessed. Chemical characterization of these extracts suggested the presence of triterpenoid saponins and spermidine phenolamides, which were not previously reported in pracaxi nuts. These results suggest that pracaxi oil extraction by-products are a valuable source of bioactive compounds with neuroprotective potential.

Published
2023

9. An alternative exploitation of Synechocystis sp. PCC6803: A cascade approach for the recovery of high added-value products

Author

Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Imbimbo, Paola [0000-0002-5491-2297], Álvarez-Rivera, Gerardo [0000-0003-1657-8141], Ibáñez, Elena [0000-0003-2127-8303], Santos, Filipe Branco dos [0000-0002-4268-8080], Monti, Daria Maria [0000-0002-1136-0576], Imbimbo, Paola, D’Elia, Luigi, Corrado, Iolanda, Álvarez-Rivera, Gerardo, Marzocchella, Antonio, Ibáñez, Elena, Pezzella, Cinzia, Santos, Filipe Branco dos, Monti, Daria Maria, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Imbimbo, Paola [0000-0002-5491-2297], Álvarez-Rivera, Gerardo [0000-0003-1657-8141], Ibáñez, Elena [0000-0003-2127-8303], Santos, Filipe Branco dos [0000-0002-4268-8080], Monti, Daria Maria [0000-0002-1136-0576], Imbimbo, Paola, D’Elia, Luigi, Corrado, Iolanda, Álvarez-Rivera, Gerardo, Marzocchella, Antonio, Ibáñez, Elena, Pezzella, Cinzia, Santos, Filipe Branco dos, and Monti, Daria Maria

Abstract

Microalgal biomass represents a very interesting biological feedstock to be converted into several high-value products in a biorefinery approach. In this study, the cyanobacterium Synechocystis sp. PCC6803 was used to obtain different classes of molecules: proteins, carotenoids and lipids by using a cascade approach. In particular, the protein extract showed a selective cytotoxicity towards cancer cells, whereas carotenoids were found to be active as antioxidants both in vitro and on a cell-based model. Finally, for the first time, lipids were recovered from Synechocystis biomass as the last class of molecules and were successfully used as an alternative substrate for the production of polyhydroxyalkanoate (PHA) by the native PHA producer Pseudomonas resinovorans. Taken together, our results lead to a significant increase in the valorization of Synechocystis sp. PCC6803 biomass, thus allowing a possible offsetting of the process costs.

Published
2023

10. Gas-Expanded Liquids Extraction

Author

Universidad de Nariño, Consejo Superior de Investigaciones Científicas (España), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Suárez Montenegro, Zully J., Abderrezag, Norelhouda, Ibáñez, Elena, Mendiola, J. A., Universidad de Nariño, Consejo Superior de Investigaciones Científicas (España), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Suárez Montenegro, Zully J., Abderrezag, Norelhouda, Ibáñez, Elena, and Mendiola, J. A.

Abstract

Gas Expanded Liquids (GXL) are mixtures of liquid solvents (organic, water) and gases or supercritical fluids with diverse physicochemical properties halfway between pure liquids and supercritical fluids. The possibility of changing their properties by introducing small changes in pressure, temperature, and/or solvent/gas ratio, makes these solvents a very interesting and appropriate option for developing green extraction protocols for food analysis. In general, GXLs have similar densities as the solvent used in their composition, while having improved mass transfer through reduced viscosity, increased solute diffusivity, and decreased interfacial tension. Some other advantages are related to the wide range of polarities that can be obtained, depending on the liquid selected. Moreover, the substitution of a liquid fraction for a gas reduces the final use of organic solvent, thus improving the green character of GXLs. In the present chapter, the physicochemical properties of GXL are addressed along together with the description of applications in the food science and technology area.

Published
2023

11. Supporting Information: Inside out porphyridium cruentum: beyond the conventional biorefinery concept

Author

Liberti, Davide, Imbimbo, Paola, Giustino, Enrica, D’Elia, Luigi, Ferraro, Giarita, Casillo, Angela, Illiano, Anna, Pinto, Gabriella, Chiara Di Meo, Maria, Álvarez-Rivera, Gerardo, Corsaro, Maria Michela, Amoresano, Angela, Zarrelli, Armando, Ibáñez, Elena, Merlino, Antonello, Monti, Daria Maria, Liberti, Davide, Imbimbo, Paola, Giustino, Enrica, D’Elia, Luigi, Ferraro, Giarita, Casillo, Angela, Illiano, Anna, Pinto, Gabriella, Chiara Di Meo, Maria, Álvarez-Rivera, Gerardo, Corsaro, Maria Michela, Amoresano, Angela, Zarrelli, Armando, Ibáñez, Elena, Merlino, Antonello, and Monti, Daria Maria

Published
2023

12. Supplementary materials An alternative exploitation of Synechocystis sp. PCC6803: a cascade approach for the recovery of high added-value products

Author

Imbimbo, Paola, D’Elia, Luigi, Corrado, Iolanda, Álvarez-Rivera, Gerardo, Marzocchella, Antonio, Ibáñez, Elena, Pezzella, Cinzia, Santos, Filipe Branco dos, Monti, Daria Maria, Imbimbo, Paola, D’Elia, Luigi, Corrado, Iolanda, Álvarez-Rivera, Gerardo, Marzocchella, Antonio, Ibáñez, Elena, Pezzella, Cinzia, Santos, Filipe Branco dos, and Monti, Daria Maria

Published
2023

14. NADEs as promising solvents in high-pressure extraction (PLE) of bioactives from avocado residues

Author

Grisales-Mejía, Juan Felipe, Ortega, J. P., Cedeño-Fierro, V., Torres-Castañeda, Harlen G., Andrade-Mahecha, Margarita M., Martínez-Correa, H. A., Mendiola, J. A., Cifuentes, Alejandro, Ibáñez, Elena, Grisales-Mejía, Juan Felipe, Ortega, J. P., Cedeño-Fierro, V., Torres-Castañeda, Harlen G., Andrade-Mahecha, Margarita M., Martínez-Correa, H. A., Mendiola, J. A., Cifuentes, Alejandro, and Ibáñez, Elena

Published
2023

15. Evaluation of the neuroprotective effect of extracts obtained by PLE with biobased solvents from Tetraselmis chuii as a novel food

Author

Ministerio de Ciencia, Innovación y Universidades (España), Ministerio de Ciencia e Innovación (España), Ministerio de Economía y Competitividad (España), Agencia Estatal de Investigación (España), Cokdinleyen, Melis, Mendiola, J. A., Álvarez-Rivera, Gerardo, Valdés, Alberto, Cifuentes, Alejandro, Ibáñez, Elena, Ministerio de Ciencia, Innovación y Universidades (España), Ministerio de Ciencia e Innovación (España), Ministerio de Economía y Competitividad (España), Agencia Estatal de Investigación (España), Cokdinleyen, Melis, Mendiola, J. A., Álvarez-Rivera, Gerardo, Valdés, Alberto, Cifuentes, Alejandro, and Ibáñez, Elena

Published
2023

16. Antioxidant and potential neuroprotective activity of Heliotropium taltalense I.M. Johnst (Heliotropiaceae) extracts obtained by pressurized liquid extraction

Author

Barrientos, Ruth E., Álvarez-Rivera, Gerardo, Mohammadnezhad, Pouya, Cifuentes, Fredi, Palacios, Javier, Paredes, Adrián, Mendiola, J. A., Simirgiotis, Mario Juan, Ibáñez, Elena, Barrientos, Ruth E., Álvarez-Rivera, Gerardo, Mohammadnezhad, Pouya, Cifuentes, Fredi, Palacios, Javier, Paredes, Adrián, Mendiola, J. A., Simirgiotis, Mario Juan, and Ibáñez, Elena

Published
2023

17. Extraction of neuroprotective compounds from Eucalyptus leaves using green technologies. Ternary mixture design

Author

Ministerio de Ciencia, Innovación y Universidades (España), Ministerio de Ciencia e Innovación (España), European Commission, Agencia Estatal de Investigación (España), Consejo Superior de Investigaciones Científicas (España), Hasni, S., Riguene, H., Rigane, G., Ghazghazi, H., Ben Salem, R., Cifuentes, Alejandro, Mendiola, J. A., Ibáñez, Elena, Ministerio de Ciencia, Innovación y Universidades (España), Ministerio de Ciencia e Innovación (España), European Commission, Agencia Estatal de Investigación (España), Consejo Superior de Investigaciones Científicas (España), Hasni, S., Riguene, H., Rigane, G., Ghazghazi, H., Ben Salem, R., Cifuentes, Alejandro, Mendiola, J. A., and Ibáñez, Elena

Published
2023

19. Extraction of marine phenolics using compressed fluids

Author

Montero, Lidia, Socas-Rodríguez, Bárbara, Mendiola, J. A., Ibáñez, Elena, Montero, Lidia, Socas-Rodríguez, Bárbara, Mendiola, J. A., and Ibáñez, Elena

Abstract

The marine environment is considered an inexhaustible source of antioxidants. Marine organisms like macro- and microalgae and sponges are excellent sources of phenolic compounds that attract researchers and consumers’ interest. These marine organisms present a complex chemical and nutritional composition, where phenolic compounds are found at much lower concentrations than macronutrients. Therefore, specific extraction strategies should be applied to obtain rich phenolic extracts to establish their chemical composition and biological functionality and develop commercial products. Nowadays, there is a clear trend to apply efficient and green extraction techniques for recovering natural ingredients, like phenolic compounds. One of the most useful and successful strategies is the use of compressed fluids extraction techniques such as supercritical fluid extraction (SFE), gas expanded liquid extraction (GXL), and pressurized liquid extraction (PLE). This chapter describes and discusses the fundamentals of these alternative green techniques and their applications to extract phenolic compounds from marine sources.

Published
2023

20. Editorial: Insights in Nutrition and Food Science Technology

Author

Ministerio de Ciencia, Innovación y Universidades (España), Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), Ibáñez, Elena, Cifuentes, Alejandro, Ministerio de Ciencia, Innovación y Universidades (España), Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), Ibáñez, Elena, and Cifuentes, Alejandro

Published
2023

21. Compositional analysis of foods

Author

Herrero, Miguel, Socas-Rodríguez, Bárbara, Sánchez-Martínez, J. David, Ibáñez, Elena, Cifuentes, Alejandro, Herrero, Miguel, Socas-Rodríguez, Bárbara, Sánchez-Martínez, J. David, Ibáñez, Elena, and Cifuentes, Alejandro

Abstract

Current applications and most recent trends in the analysis of foods and food components using liquid chromatography (LC) are presented in this chapter. Although LC is a widely extended analytical tool for food analysis within different fields (safety, quality, and traceability), this work is mainly focused on the analysis of food constituents, paying special attention to the analysis of carbohydrates, vitamins, amino acids, peptides, proteins, and lipids, as well as other minor food compounds (e.g., polyphenols and carotenoids) and food additives used as preservatives, antioxidants, colorants, or sweeteners. The text includes fundamentals about their physiological roles together with sample preparation and LC conditions most typically used for their analysis in foods.

Published
2023

22. Contribution of phenolics and free amino acids on the antioxidant profile of commercial lemon verbena infusions

Author

European Commission, Programa Operacional do Potencial Humano (Portugal), Fundação para a Ciência e a Tecnologia (Portugal), Ministério da Ciência, Tecnologia e Ensino Superior (Portugal), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia e Innovación (España), Barreto Peixoto, Juliana A., Álvarez-Rivera, Gerardo, Costa, Anabela S. G., Machado, Susana, Cifuentes, Alejandro, Ibáñez, Elena, Oliveira, Beatriz P.P., Alves, Rita C., European Commission, Programa Operacional do Potencial Humano (Portugal), Fundação para a Ciência e a Tecnologia (Portugal), Ministério da Ciência, Tecnologia e Ensino Superior (Portugal), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia e Innovación (España), Barreto Peixoto, Juliana A., Álvarez-Rivera, Gerardo, Costa, Anabela S. G., Machado, Susana, Cifuentes, Alejandro, Ibáñez, Elena, Oliveira, Beatriz P.P., and Alves, Rita C.

Abstract

Lemon verbena infusions are widely appreciated due to their agreeable lemony flavor and medicinal properties. In this study, the antioxidant potential, phenolic profile, and free amino acid profile of lemon verbena infusions from different commercial brands were studied. Characterization by UHPLC-QTOF-HRMS allowed the identification of 34 phenolics. The free amino acid profile (by RP-HPLC-FLD) was assessed for the first time, allowing the quantification of 16 amino acids. Furthermore, the infusions showed high antioxidant activity by different assays (ferric reducing antioxidant power, DPPH• scavenging, and oxygen radical absorbance capacity assays), which in turn were significantly correlated with total phenolics and total flavonoid contents. Notwithstanding, phenylalanine seemed to have also an impact on the antioxidant activity of the infusions, with significant correlations found. Finally, significant differences were found in all the evaluated parameters for one of the four commercial brands herein studied, which was possibly related to the different geographical origins of this sample. Overall, these lemon verbena infusions proved to be rich in a huge variety of bioactive compounds that can provide therapeutic potential.

Published
2023

23. Mapping Protein Targets of Carnosol, a Molecule Identified in Rosmarinus officinalis: In Silico Docking Studies and Network Pharmacology

Author

Taboada-Alquerque, María, Pajaro-Valenzuela, Danilo, Caballero-Gallardo, Karina, Cifuentes, Alejandro, Ibáñez, Elena, Ahumedo-Monterrosa, Maicol, Stashenko, Elena E., Olivero-Verbel, Jesus, Taboada-Alquerque, María, Pajaro-Valenzuela, Danilo, Caballero-Gallardo, Karina, Cifuentes, Alejandro, Ibáñez, Elena, Ahumedo-Monterrosa, Maicol, Stashenko, Elena E., and Olivero-Verbel, Jesus

Abstract

Carnosol is a natural diterpene present in Rosmarinus officinalis L. (rosemary) with anti-tumor and anti-inflammatory properties. Despite its importance, the pharmacological mechanisms underlying the interactions between carnosol and human targets are still unclear. The goal was to identify plausible human target for carnosol and the network pharmacology. Rosemary was analyzed using HPLC-QTOF-MS/MS. Potential carnosol targets were identified using docking and a public database (CTD). Carnosol was screened against 708 human proteins using AutoDock Vina, and affinity values were used as prioritization criteria. The targets set was uploaded to WebGestalt to obtain Gene Ontology (GO) and KEGG pathway enrichment analysis. HPLC-QTOF-MS/MS analyses allowed the tentative annotation of nine chemicals, with carnosol being the most ionized. There were 53 plausible targets for carnosol, with 20 identified using virtual screening, including Hsp90α (−10.9 kcal/mol), AKR1C3 (−10.4 kcal/mol), and Hsp90β (−10.4 kcal/mol), and 33 identified from CTD. The potential targets for carnosol identified with PPI and molecular docking were HSP90AA1, MAPK1, MAPK3, CAT, JUN, AHR, and CASP3. GO terms and KEGG pathways analysis found that carnosol is closely related to infection (Chagas, influenza A, toxoplasmosis, and pertussis) and inflammation (IL-17 and TNF signaling pathway and Th-17 cell differentiation). These results demonstrated that carnosol may induce an immuno-inflammatory response.

Published
2023

24. Editorial: Insights into food science and technology in Latin America

Author

Sánchez-Camargo, A. P., Gutiérrez-Uribe, Janet A., Silva, María Fernanda, Salvador Ferreira, Sandra Regina, Cifuentes, Alejandro, Ibáñez, Elena, Sánchez-Camargo, A. P., Gutiérrez-Uribe, Janet A., Silva, María Fernanda, Salvador Ferreira, Sandra Regina, Cifuentes, Alejandro, and Ibáñez, Elena

Published
2023

25. Bacterial carotenoids: Extraction, characterization, and applications

Author

Ministerio de Ciencia, Tecnología e Innovación (Colombia), Universidad de Los Andes (Colombia), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), López, Gerson-Dirceu, Álvarez-Rivera, Gerardo, Carazzone, Chiara, Ibáñez, Elena, Leidy, Chad, Cifuentes, Alejandro, Ministerio de Ciencia, Tecnología e Innovación (Colombia), Universidad de Los Andes (Colombia), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), López, Gerson-Dirceu, Álvarez-Rivera, Gerardo, Carazzone, Chiara, Ibáñez, Elena, Leidy, Chad, and Cifuentes, Alejandro

Abstract

Natural carotenoids are secondary metabolites that exhibit antioxidant, anti-inflammatory, and anti-cancer properties. These types of compounds are highly demanded by pharmaceutical, cosmetic, nutraceutical, and food industries, leading to the search for new natural sources of carotenoids. In recent years, the production of carotenoids from bacteria has become of great interest for industrial applications. In addition to carotenoids with C40-skeletons, some bacteria have the ability to synthesize characteristic carotenoids with C30-skeletons. In this regard, a great variety of methodologies for the extraction and identification of bacterial carotenoids has been reported and this is the first review that condenses most of this information. To understand the diversity of carotenoids from bacteria, we present their biosynthetic origin in order to focus on the methodologies employed in their extraction and characterization. Special emphasis has been made on high-performance liquid chromatography-mass spectrometry (HPLC-MS) for the analysis and identification of bacterial carotenoids. We end up this review showing their potential commercial use. This review is proposed as a guide for the identification of these metabolites, which are frequently reported in new bacteria strains.

Published
2023

26. A smartphone-powered photoelectrochemical POCT via Z-scheme Cu2O/Cu3SnS4 for dibutyl phthalate in the environmental and food

Author

National Natural Science Foundation of China, Hubei Provincial Natural Science Foundation, Chen, Xiao, Wu, Wenqin, Zeng, Jing, Ibáñez, Elena, Cifuentes, Alejandro, Mao, Jin, Yu, Li, Wu, Huimin, Li, Peiwu, Zhang, Zhaowei, National Natural Science Foundation of China, Hubei Provincial Natural Science Foundation, Chen, Xiao, Wu, Wenqin, Zeng, Jing, Ibáñez, Elena, Cifuentes, Alejandro, Mao, Jin, Yu, Li, Wu, Huimin, Li, Peiwu, and Zhang, Zhaowei

Abstract

As a major hazardous additive released from microplastics and nanoplastics, identifying dibutyl phthalate (DBP) in complex matrices attracts a growing concern in environmental monitoring and food safety. For the first time, Cu2O/Cu3SnS4 nanoflower is prepared and serves as the photoactive material which can be constructed as a smartphone-based photoelectrochemical (PEC) point-of-care test (POCT). Effectively matching energy levels between Cu2O and Cu3SnS4 accelerated the transfer of photogenerated electron-hole pairs, significantly improving the intelligent PEC POCT performance. The novel Cu2O/Cu3SnS4 has proven to be the Z-scheme heterojunction by density functional theory calculation. A competitive immunoassay has been realized on a Cu2O/Cu3SnS4 modified electrode, dramatically decreasing the photocurrent signal and enhancing POCT sensitivity. The smartphone has been used to record and transfer PEC results. Under optimal conditions, the PEC POCT exhibited a satisfying linear range (0.04–400 ng/mL) and a low detection limit of 7.94 pg/mL in real samples, together with excellent stability, repeatability, reproducibility and selectivity. The PEC POCT system provides good performance and practicability in determining DBP in water and edible oil samples. This proposal provides a practical strategy for the intelligent POCT for environment monitoring and food safety.

Published
2023

28. Supplementary Materials for Phenolic fingerprinting and bioactivity profiling of extracts and isolated compounds from Gypothamnium pinifolium Phil.

Author

Barrientos, Ruth E., Ibáñez, Elena, Puerta, Adrián, Padrón, José M., Paredes, Adrián, Cifuentes, Fredi, Romero-Parra, Javier, Palacios, Javier, Bórquez, Jorge, Simirgiotis, Mario Juan, Barrientos, Ruth E., Ibáñez, Elena, Puerta, Adrián, Padrón, José M., Paredes, Adrián, Cifuentes, Fredi, Romero-Parra, Javier, Palacios, Javier, Bórquez, Jorge, and Simirgiotis, Mario Juan

Published
2022

31. Synergic effect of natural deep eutectic solvent and high-intensity ultrasound on obtaining a ready-to-use genipin extract: Crosslinking and anti-neurodegenerative properties: Appendix A. Supplementary data

Author

Landim Neves, Maria Isabel, Socas-Rodríguez, Bárbara, Valdés, Alberto, Keven Silva, Eric, Cifuentes, Alejandro, Meireles, Maria Angela A., Ibáñez, Elena, Landim Neves, Maria Isabel, Socas-Rodríguez, Bárbara, Valdés, Alberto, Keven Silva, Eric, Cifuentes, Alejandro, Meireles, Maria Angela A., and Ibáñez, Elena

Published
2022

32. Neuroprotective potential of terpenoid-rich extracts from orange juice by-products obtained by pressurized liquid extraction: Appendix A. Supplementary data

Author

Sánchez-Martínez, J. David, Álvarez-Rivera, Gerardo, Gallego, Rocío, Bittencourt Fagundes, Mariane, Valdés, Alberto, Mendiola, J. A., Ibáñez, Elena, Cifuentes, Alejandro, Sánchez-Martínez, J. David, Álvarez-Rivera, Gerardo, Gallego, Rocío, Bittencourt Fagundes, Mariane, Valdés, Alberto, Mendiola, J. A., Ibáñez, Elena, and Cifuentes, Alejandro

Published
2022

36. Synergic effect of natural deep eutectic solvent and high-intensity ultrasound on obtaining a ready-to-use genipin extract: Crosslinking and anti-neurodegenerative properties

Author

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Landim Neves, Maria Isabel, Socas-Rodríguez, Bárbara, Valdés, Alberto, Keven Silva, Eric, Cifuentes, Alejandro, Meireles, Maria Angela A., Ibáñez, Elena, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Landim Neves, Maria Isabel, Socas-Rodríguez, Bárbara, Valdés, Alberto, Keven Silva, Eric, Cifuentes, Alejandro, Meireles, Maria Angela A., and Ibáñez, Elena

Abstract

In this paper, genipin, an important natural crosslinker and anti-neurodegenerative compound, was extracted from unripe Genipa americana L., combining high-intensity ultrasound (HIUS) and natural deep eutectic solvents (NADESs). The extraction process conditions were evaluated step-by-step to reach the best genipin recovery. The obtained ready-to-use genipin-NADES extract was examined regarding its crosslinking properties and anti-neurodegenerative capacity. For the condictions tested, the highest genipin recovery was obtained using 40 % water and 60 % betaine:lactic acid NADES in molar ratio 1:3 (n/n) as the solvent, a solvent:feed ratio of 19 (w/w), and HIUS acoustic power of 14 ± 1 W. The HIUS-assisted extraction using NADES as solvent showed to be a promising and efficient green extraction technique to obtain genipin. The ready-to-use genipin-NADES extract presented crosslinking capacity and anticholinergic activity. These results indicate that genipin-NADES extract can be directly applied in hydrogels for drug delivery, films, tissue engineering, and others. Moreover, it can be used in food, supplements, and medicine to enhance their neuroprotective effect.

Published
2022

37. Editorial: Women in nutrition and food science technology

Author

Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ibáñez, Elena, Montero, Lidia, Sánchez-Camargo, A. P., Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ibáñez, Elena, Montero, Lidia, and Sánchez-Camargo, A. P.

Published
2022

38. Metabolomic fingerprinting and biological activities of Gypothamnium pinifolium Phil. from Northern Chile

Author

Fondo Nacional de Desarrollo Científico y Tecnológico (Chile), Agencia Nacional de Investigación y Desarrollo (Chile), Universidad de Antofagasta, Barrientos, Ruth E., Álvarez-Rivera, Gerardo, Ibáñez, Elena, Mendiola, J. A., Paredes, Adrián, Cifuentes, Fredi, Palacios, Javier, Simirgiotis, Mario Juan, Fondo Nacional de Desarrollo Científico y Tecnológico (Chile), Agencia Nacional de Investigación y Desarrollo (Chile), Universidad de Antofagasta, Barrientos, Ruth E., Álvarez-Rivera, Gerardo, Ibáñez, Elena, Mendiola, J. A., Paredes, Adrián, Cifuentes, Fredi, Palacios, Javier, and Simirgiotis, Mario Juan

Published
2022

39. Compressed fluids as sample preparation for the analysis of antioxidant and neuroprotective compounds from Avocado (peresea americana, var Hass) epicarp with a biorefinery approach

Author

Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Consejo Superior de Investigaciones Científicas (España), Universidad Nacional de Colombia, Grisales-Mejía, Juan Felipe, Vega Gómez, Laura M., Mendiola, J. A., Álvarez-Rivera, Gerardo, Torres-Castañeda, Harlen G., Martínez-Correa, H. A., Andrade-Mahecha, Margarita M., Cifuentes, Alejandro, Ibáñez, Elena, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Consejo Superior de Investigaciones Científicas (España), Universidad Nacional de Colombia, Grisales-Mejía, Juan Felipe, Vega Gómez, Laura M., Mendiola, J. A., Álvarez-Rivera, Gerardo, Torres-Castañeda, Harlen G., Martínez-Correa, H. A., Andrade-Mahecha, Margarita M., Cifuentes, Alejandro, and Ibáñez, Elena

Published
2022

40. Extraction and evaluation of natural bioactive compounds through green foodomics

Author

Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Gallego, Rocío, Sánchez-Martínez, J. David, Álvarez-Rivera, Gerardo, Mendiola, J. A., Valdés, Alberto, Cifuentes, Alejandro, Ibáñez, Elena, Herrero, Miguel, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Gallego, Rocío, Sánchez-Martínez, J. David, Álvarez-Rivera, Gerardo, Mendiola, J. A., Valdés, Alberto, Cifuentes, Alejandro, Ibáñez, Elena, and Herrero, Miguel

Published
2022

41. LC-HRMS based metabolomics to understand mercury tolerance in plants: Are flavonoids doing the trick?

Author

Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Álvarez-Rivera, Gerardo, Sanz, Aurora, Cifuentes, Alejandro, Ibáñez, Elena, Paape, Timothy, Lucas, M. Mercedes, Pueyo, José Javier, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Álvarez-Rivera, Gerardo, Sanz, Aurora, Cifuentes, Alejandro, Ibáñez, Elena, Paape, Timothy, Lucas, M. Mercedes, and Pueyo, José Javier

Published
2022

43. Compressed fluids extraction and purification of neuroprotective compounds from Tetraselmis chuii

Author

Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Cokdinleyen, Melis, Sánchez-Martínez, J. David, Mendiola, J. A., Álvarez-Rivera, Gerardo, Cifuentes, Alejandro, Ibáñez, Elena, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Cokdinleyen, Melis, Sánchez-Martínez, J. David, Mendiola, J. A., Álvarez-Rivera, Gerardo, Cifuentes, Alejandro, and Ibáñez, Elena

Published
2022

44. Exploring the neuroprotective potential of artichoke by-products by pressurized liquid extraction coupled to LC-QTOF-MS/MS

Author

Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Vega Gómez, Laura M., Sánchez-Martínez, J. David, Mendiola, J. A., Ibáñez, Elena, Cifuentes, Alejandro, Álvarez-Rivera, Gerardo, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Vega Gómez, Laura M., Sánchez-Martínez, J. David, Mendiola, J. A., Ibáñez, Elena, Cifuentes, Alejandro, and Álvarez-Rivera, Gerardo

Published
2022

47. Gas and liquid chromatography coupled to high-resolution mass spectrometry to evaluate the neuroprotective potential of natural extracts

Author

Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Educación y Ciencia (España), Sánchez-Martínez, J. David, Álvarez-Rivera, Gerardo, Valdés, Alberto, Mendiola, J. A., Herrero, Miguel, Ibáñez, Elena, Cifuentes, Alejandro, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Educación y Ciencia (España), Sánchez-Martínez, J. David, Álvarez-Rivera, Gerardo, Valdés, Alberto, Mendiola, J. A., Herrero, Miguel, Ibáñez, Elena, and Cifuentes, Alejandro

Published
2022

48. Transcriptomics and metabolomics evaluation of the in vivo neuroprotective potential of a Dunaliella salina microalgae extract

Author

Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Valdés, Alberto, Gallego, Rocío, Ibáñez, Elena, Herrero, Miguel, Cifuentes, Alejandro, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Valdés, Alberto, Gallego, Rocío, Ibáñez, Elena, Herrero, Miguel, and Cifuentes, Alejandro

Published
2022

49. Activity of natural compounds against Alzheimer investigated by foodomics

Author

Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Valdés, Alberto, Álvarez-Rivera, Gerardo, Mendiola, J. A., Herrero, Miguel, Ibáñez, Elena, Cifuentes, Alejandro, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Valdés, Alberto, Álvarez-Rivera, Gerardo, Mendiola, J. A., Herrero, Miguel, Ibáñez, Elena, and Cifuentes, Alejandro

Published
2022

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