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32 results on '"Gutiérrez-Gallego R"'

1. Evaluation of protein N-glycosylation in 2-DE: Erythropoietin as a study case

Author

Llop, E., Gutiérrez Gallego, R., Belalcazar, V., Gerwig, G.J., Kamerling, J.P., Segura, J., Pascual, J.A., Llop, E., Gutiérrez Gallego, R., Belalcazar, V., Gerwig, G.J., Kamerling, J.P., Segura, J., and Pascual, J.A.

Abstract

The structure, function, and physico-chemical properties of many proteins are determined by PTM, being glycosylation the most complex. This study describes how a combination of typical proteomics methods (2-DE) combines with glycomics strategies (HPLC, MALDI-TOF-MS, exoglycosidases sequencing) to yield comprehensive data about single spot-microheterogeneity, providing meaningful information for the detection of disease markers, pharmaceutical industry, antidoping control, etc. Recombinant erythropoietin and its hyperglycosylated analogue darbepoetin- a were chosen as showcases because of their relevance in these fields and the analytical challenge they represent. The combined approach yielded good results in terms of sample complexity (mixture glycoforms), reproducibility, sensitivity (~25 pmoles of glycoprotein/spot), and identification of the underlying protein. Heterogeneity was present in all spots but with a clear tendency; spots proximal to the anode contained the highest amount of tetra-antennary tetrasialylated glycans, whereas the opposite occurred for spots proximal to the cathode with the majority of the structures being undersialylated. Spot microheterogeneity proved a consequence of the multiple glycosylation sites as they contributed directly to the number of possibilities to account for a discrete charge in a single spot. The interest of this combined glycoproteomics method resides in the efficiency for detecting and quantifying subtle dissimilarities originated from altered ratios of identical glycans including N-acetyl-lactosamine repeats, acetylation, or antigenic epitopes, that do not significantly contribute to the electrophoretic mobility, but affect the glycan microheterogeneity and the potential underlying related functionality.

Published
2007

2. Evaluation of protein N-glycosylation in 2-DE: Erythropoietin as a study case

Author

Chemie van glyco-conjugaten, Dep Scheikunde, Llop, E., Gutiérrez Gallego, R., Belalcazar, V., Gerwig, G.J., Kamerling, J.P., Segura, J., Pascual, J.A., Chemie van glyco-conjugaten, Dep Scheikunde, Llop, E., Gutiérrez Gallego, R., Belalcazar, V., Gerwig, G.J., Kamerling, J.P., Segura, J., and Pascual, J.A.

Published
2007

3. Evaluation of protein N-glycosylation in 2-DE: Erythropoietin as a study case

Author

Chemie van glyco-conjugaten, Dep Scheikunde, Llop, E., Gutiérrez Gallego, R., Belalcazar, V., Gerwig, G.J., Kamerling, J.P., Segura, J., Pascual, J.A., Chemie van glyco-conjugaten, Dep Scheikunde, Llop, E., Gutiérrez Gallego, R., Belalcazar, V., Gerwig, G.J., Kamerling, J.P., Segura, J., and Pascual, J.A.

Published
2007

4. Evaluation of protein N-glycosylation in 2-DE: Erythropoietin as a study case

Author

Chemie van glyco-conjugaten, Dep Scheikunde, Llop, E., Gutiérrez Gallego, R., Belalcazar, V., Gerwig, G.J., Kamerling, J.P., Segura, J., Pascual, J.A., Chemie van glyco-conjugaten, Dep Scheikunde, Llop, E., Gutiérrez Gallego, R., Belalcazar, V., Gerwig, G.J., Kamerling, J.P., Segura, J., and Pascual, J.A.

Published
2007

11. Epitope diversity of N-glycans from bovine peripheral myelin glycoprotein P0 revealed by mass spectrometry and nano probe magic angle spinning 1H NMR spectroscopy

Author

Vliegenthart, J.F.G., Gutiérrez Gallego, R., Jiménez Blanco, J.L., Thijssen-van Zuylen, C.W.E.M., Gotfredsen, C.H., Voshol, H., Duus, J.Ø., Schachner, M., Vliegenthart, J.F.G., Gutiérrez Gallego, R., Jiménez Blanco, J.L., Thijssen-van Zuylen, C.W.E.M., Gotfredsen, C.H., Voshol, H., Duus, J.Ø., and Schachner, M.

Published
2001

14. Epitope diversity of N-glycans from bovine peripheral myelin glycoprotein P0 revealed by mass spectrometry and nano probe magic angle spinning 1H NMR spectroscopy

Author

Vliegenthart, J.F.G., Gutiérrez Gallego, R., Jiménez Blanco, J.L., Thijssen-van Zuylen, C.W.E.M., Gotfredsen, C.H., Voshol, H., Duus, J.Ø., Schachner, M., Vliegenthart, J.F.G., Gutiérrez Gallego, R., Jiménez Blanco, J.L., Thijssen-van Zuylen, C.W.E.M., Gotfredsen, C.H., Voshol, H., Duus, J.Ø., and Schachner, M.

Published
2001

16. Epitope diversity of N-glycans from bovine peripheral myelin glycoprotein P0 revealed by mass spectrometry and nano probe magic angle spinning 1H NMR spectroscopy

Author

Vliegenthart, J.F.G., Gutiérrez Gallego, R., Jiménez Blanco, J.L., Thijssen-van Zuylen, C.W.E.M., Gotfredsen, C.H., Voshol, H., Duus, J.Ø., Schachner, M., Vliegenthart, J.F.G., Gutiérrez Gallego, R., Jiménez Blanco, J.L., Thijssen-van Zuylen, C.W.E.M., Gotfredsen, C.H., Voshol, H., Duus, J.Ø., and Schachner, M.

Published
2001

18. Epitope diversity of N-glycans from bovine peripheral myelin glycoprotein P0 revealed by mass spectrometry and nano probe magic angle spinning 1H NMR spectroscopy

Author

Vliegenthart, J.F.G., Gutiérrez Gallego, R., Jiménez Blanco, J.L., Thijssen-van Zuylen, C.W.E.M., Gotfredsen, C.H., Voshol, H., Duus, J.Ø., Schachner, M., Vliegenthart, J.F.G., Gutiérrez Gallego, R., Jiménez Blanco, J.L., Thijssen-van Zuylen, C.W.E.M., Gotfredsen, C.H., Voshol, H., Duus, J.Ø., and Schachner, M.

Published
2001

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