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1. Talaromyces borbonicus, sp. nov., a novel fungus from biodegraded Arundo donax with potential abilities in lignocellulose conversion.

Author

Varriale, Simona, Varriale, Simona, Houbraken, Jos, Granchi, Zoraide, Pepe, Olimpia, Cerullo, Gabriella, Ventorino, Valeria, Chin-A-Woeng, Thomas, Meijer, Martin, Riley, Robert, Grigoriev, Igor V, Henrissat, Bernard, de Vries, Ronald P, Faraco, Vincenza, Varriale, Simona, Varriale, Simona, Houbraken, Jos, Granchi, Zoraide, Pepe, Olimpia, Cerullo, Gabriella, Ventorino, Valeria, Chin-A-Woeng, Thomas, Meijer, Martin, Riley, Robert, Grigoriev, Igor V, Henrissat, Bernard, de Vries, Ronald P, and Faraco, Vincenza

Abstract

A novel fungal species able to synthesize enzymes with potential synergistic actions in lignocellulose conversion was isolated from the biomass of Arundo donax during biodegradation under natural conditions in the Gussone Park of the Royal Palace of Portici (Naples, Italy). In this work, this species was subjected to morphological and phylogenetic analyses. Sequencing of its genome was performed, resulting in 28 scaffolds that were assembled into 27.05 Mb containing 9744 predicted genes, among which 396 belong to carbohydrate-active enzyme (CAZyme)-encoding genes. Here we describe and illustrate this previously unknown species, which was named Talaromyces borbonicus, by a polyphasic approach combining phenotypic, physiological, and sequence data.

Published
2018

2. Fungal glucuronoyl esterases: Genome mining based enzyme discovery and biochemical characterization

Author

Dilokpimol, Adiphol, Mäkelä, Miia R, Cerullo, Gabriella, Zhou, Miaomiao, Varriale, Simona, Gidijala, Loknath, Brás, Joana L A, Jütten, Peter, Piechot, Alexander, Verhaert, Raymond, Faraco, Vincenza, Hilden, Kristiina S., de Vries, Ronald P, Dilokpimol, Adiphol, Mäkelä, Miia R, Cerullo, Gabriella, Zhou, Miaomiao, Varriale, Simona, Gidijala, Loknath, Brás, Joana L A, Jütten, Peter, Piechot, Alexander, Verhaert, Raymond, Faraco, Vincenza, Hilden, Kristiina S., and de Vries, Ronald P

Abstract

4-O-Methyl-d-glucuronic acid (MeGlcA) is a side-residue of glucuronoarabinoxylan and can form ester linkages to lignin, contributing significantly to the strength and rigidity of the plant cell wall. Glucuronoyl esterases (4-O-methyl-glucuronoyl methylesterases, GEs) can cleave this ester bond, and therefore may play a significant role as auxiliary enzymes in biomass saccharification for the production of biofuels and biochemicals. GEs belong to a relatively new family of carbohydrate esterases (CE15) in the CAZy database (www.cazy.org), and so far around ten fungal GEs have been characterized. To explore additional GE enzymes, we used a genome mining strategy. BLAST analysis with characterized GEs against approximately 250 publicly accessible fungal genomes identified more than 150 putative fungal GEs, which were classified into eight phylogenetic sub-groups. To validate the genome mining strategy, 21 selected GEs from both ascomycete and basidiomycete fungi were heterologously produced in Pichia pastoris. Of these enzymes, 18 were active against benzyl d-glucuronate demonstrating the suitability of our genome mining strategy for enzyme discovery.

Published
2018

3. Fungal glucuronoyl esterases: Genome mining based enzyme discovery and biochemical characterization

Author

Dilokpimol, Adiphol, Mäkelä, Miia R, Cerullo, Gabriella, Zhou, Miaomiao, Varriale, Simona, Gidijala, Loknath, Brás, Joana L A, Jütten, Peter, Piechot, Alexander, Verhaert, Raymond, Faraco, Vincenza, Hilden, Kristiina S., de Vries, Ronald P, Dilokpimol, Adiphol, Mäkelä, Miia R, Cerullo, Gabriella, Zhou, Miaomiao, Varriale, Simona, Gidijala, Loknath, Brás, Joana L A, Jütten, Peter, Piechot, Alexander, Verhaert, Raymond, Faraco, Vincenza, Hilden, Kristiina S., and de Vries, Ronald P

Abstract

4-O-Methyl-d-glucuronic acid (MeGlcA) is a side-residue of glucuronoarabinoxylan and can form ester linkages to lignin, contributing significantly to the strength and rigidity of the plant cell wall. Glucuronoyl esterases (4-O-methyl-glucuronoyl methylesterases, GEs) can cleave this ester bond, and therefore may play a significant role as auxiliary enzymes in biomass saccharification for the production of biofuels and biochemicals. GEs belong to a relatively new family of carbohydrate esterases (CE15) in the CAZy database (www.cazy.org), and so far around ten fungal GEs have been characterized. To explore additional GE enzymes, we used a genome mining strategy. BLAST analysis with characterized GEs against approximately 250 publicly accessible fungal genomes identified more than 150 putative fungal GEs, which were classified into eight phylogenetic sub-groups. To validate the genome mining strategy, 21 selected GEs from both ascomycete and basidiomycete fungi were heterologously produced in Pichia pastoris. Of these enzymes, 18 were active against benzyl d-glucuronate demonstrating the suitability of our genome mining strategy for enzyme discovery.

Published
2018

4. Fungal glucuronoyl esterases : Genome mining based enzyme discovery and biochemical characterization

Author

Dilokpimol, Adiphol, Mäkelä, Miia R, Cerullo, Gabriella, Zhou, Miaomiao, Varriale, Simona, Gidijala, Loknath, Brás, Joana L A, Jütten, Peter, Piechot, Alexander, Verhaert, Raymond, Faraco, Vincenza, Hilden, Kristiina S, de Vries, Ronald P, Dilokpimol, Adiphol, Mäkelä, Miia R, Cerullo, Gabriella, Zhou, Miaomiao, Varriale, Simona, Gidijala, Loknath, Brás, Joana L A, Jütten, Peter, Piechot, Alexander, Verhaert, Raymond, Faraco, Vincenza, Hilden, Kristiina S, and de Vries, Ronald P

Published
2018

5. Draft Genome Sequence of Talaromyces adpressus

Author

Sub Molecular Microbiology, Sub Molecular Plant Physiology, Molecular Plant Physiology, Cerullo, Gabriella, Houbraken, Jos, Granchi, Zoraide, Pepe, Olimpia, Varriale, Simona, Ventorino, Valeria, Chin-A-Woeng, Thomas, Meijer, Martin, de Vries, Ronald P, Faraco, Vincenza, Sub Molecular Microbiology, Sub Molecular Plant Physiology, Molecular Plant Physiology, Cerullo, Gabriella, Houbraken, Jos, Granchi, Zoraide, Pepe, Olimpia, Varriale, Simona, Ventorino, Valeria, Chin-A-Woeng, Thomas, Meijer, Martin, de Vries, Ronald P, and Faraco, Vincenza

Published
2018

6. Fungal feruloyl esterases: Functional validation of genome mining based enzyme discovery including uncharacterized subfamilies

Author

Sub Molecular Plant Physiology, Molecular Plant Physiology, Dilokpimol, Adiphol, Mäkelä, Miia R, Varriale, Simona, Zhou, Miaomiao, Cerullo, Gabriella, Gidijala, Loknath, Hinkka, Harri, Brás, Joana L A, Jütten, Peter, Piechot, Alexander, Verhaert, Raymond, Hildén, Kristiina S, Faraco, Vincenza, de Vries, Ronald P, Sub Molecular Plant Physiology, Molecular Plant Physiology, Dilokpimol, Adiphol, Mäkelä, Miia R, Varriale, Simona, Zhou, Miaomiao, Cerullo, Gabriella, Gidijala, Loknath, Hinkka, Harri, Brás, Joana L A, Jütten, Peter, Piechot, Alexander, Verhaert, Raymond, Hildén, Kristiina S, Faraco, Vincenza, and de Vries, Ronald P

Published
2018

7. The Synthetic Potential of Fungal Feruloyl Esterases: A Correlation with Current Classification Systems and Predicted Structural Properties

Author

Sub Molecular Plant Physiology, Molecular Plant Physiology, Antonopoulou, Io, Dilokpimol, Adiphol, Iancu, Laura, Makela, Miia R., Varriale, Simona, Cerullo, Gabriella, Huttner, Silvia, Uthoff, Stefan, Juetten, Peter, Piechot, Alexander, Steinbuechel, Alexander, Olsson, Lisbeth, Faraco, Vincenza, Hilden, Kristiina S., de Vries, Ronald P., Rova, Ulrika, Christakopoulos, Paul, Sub Molecular Plant Physiology, Molecular Plant Physiology, Antonopoulou, Io, Dilokpimol, Adiphol, Iancu, Laura, Makela, Miia R., Varriale, Simona, Cerullo, Gabriella, Huttner, Silvia, Uthoff, Stefan, Juetten, Peter, Piechot, Alexander, Steinbuechel, Alexander, Olsson, Lisbeth, Faraco, Vincenza, Hilden, Kristiina S., de Vries, Ronald P., Rova, Ulrika, and Christakopoulos, Paul

Published
2018

8. The Synthetic Potential of Fungal Feruloyl Esterases: A Correlation with Current Classification Systems and Predicted Structural Properties

Author

Antonopoulou, Io, Dilokpimol, Adiphol, Iancu, Laura, Makela, Miia R., Varriale, Simona, Cerullo, Gabriella, Huttner, Silvia, Uthoff, Stefan, Juetten, Peter, Piechot, Alexander, Steinbuechel, Alexander, Olsson, Lisbeth, Faraco, Vincenza, Hilden, Kristiina S., de Vries, Ronald P., Rova, Ulrika, Christakopoulos, Paul, Antonopoulou, Io, Dilokpimol, Adiphol, Iancu, Laura, Makela, Miia R., Varriale, Simona, Cerullo, Gabriella, Huttner, Silvia, Uthoff, Stefan, Juetten, Peter, Piechot, Alexander, Steinbuechel, Alexander, Olsson, Lisbeth, Faraco, Vincenza, Hilden, Kristiina S., de Vries, Ronald P., Rova, Ulrika, and Christakopoulos, Paul

Published
2018

9. Draft Genome Sequence of Talaromyces adpressus

Author

Cerullo, Gabriella, Houbraken, Jos, Granchi, Zoraide, Pepe, Olimpia, Varriale, Simona, Ventorino, Valeria, Chin-A-Woeng, Thomas, Meijer, Martin, de Vries, Ronald P., Faraco, Vincenza, Cerullo, Gabriella, Houbraken, Jos, Granchi, Zoraide, Pepe, Olimpia, Varriale, Simona, Ventorino, Valeria, Chin-A-Woeng, Thomas, Meijer, Martin, de Vries, Ronald P., and Faraco, Vincenza

Published
2018

10. Talaromyces borbonicus, sp. nov., a novel fungus from biodegraded Arundo donax with potential abilities in lignocellulose conversion.

Author

Varriale, Simona, Houbraken, J., Granchi, Zoraide, Pepe, Olimpia, Cerullo, Gabriella, Ventorino, Valeria, Chin-A-Woeng, Thomas, Meijer, M., Riley, Robert, Grigoriev, Igor V, Henrissat, Bernard, de Vries, R.P., Faraco, Vincenza, Varriale, Simona, Houbraken, J., Granchi, Zoraide, Pepe, Olimpia, Cerullo, Gabriella, Ventorino, Valeria, Chin-A-Woeng, Thomas, Meijer, M., Riley, Robert, Grigoriev, Igor V, Henrissat, Bernard, de Vries, R.P., and Faraco, Vincenza

Published
2018

11. Fungal feruloyl esterases: Functional validation of genome mining based enzyme discovery including uncharacterized subfamilies

Author

Dilokpimol, Adiphol, Makela, Miia R., Varriale, Simona, Zhou, Miaomiao, Cerullo, Gabriella, Gidijala, Loknath, Hinkka, Harri, Bras, Joana L. A., Juetten, Peter, Piechot, Alexander, Verhaert, Raymond, Hilden, Kristiina S., Faraco, Vincenza, de Vries, Ronald P., Dilokpimol, Adiphol, Makela, Miia R., Varriale, Simona, Zhou, Miaomiao, Cerullo, Gabriella, Gidijala, Loknath, Hinkka, Harri, Bras, Joana L. A., Juetten, Peter, Piechot, Alexander, Verhaert, Raymond, Hilden, Kristiina S., Faraco, Vincenza, and de Vries, Ronald P.

Abstract

Feruloyl esterases (FAEs) are a diverse group of enzymes that specifically catalyze the hydrolysis of ester bonds between a hydroxycinnamic (e.g. ferulic) acid and plant poly- or oligosaccharides. FAEs as auxiliary enzymes significantly assist xylanolytic and pectinolytic enzymes in gaining access to their site of action during biomass saccharification for biofuel and biochemical production. A limited number of FAEs have been functionally characterized compared to over 1000 putative fungal FAEs that were recently predicted by similarity-based genome mining, which divided phylogenetically into different subfamilies (SFs). In this study, 27 putative and six characterized FAEs from both ascomycete and basidiomycete fungi were selected and heterologously expressed in Pichia pastoris and the recombinant proteins biochemically characterized to validate the previous genome mining and phylogenetical grouping and to expand the information on activity of fungal FAEs. As a result, 20 enzymes were shown to possess FAE activity, being active towards pNP-ferulate and/or methyl hydroxycinnamate substrates, and covering 11 subfamilies. Most of the new FAEs showed activities comparable to those of previously characterized fungal FAEs.

Published
2018

12. Fungal glucuronoyl esterases: Genome mining based enzyme discovery and biochemical characterization

Author

Sub Molecular Plant Physiology, Molecular Plant Physiology, Dilokpimol, Adiphol, Mäkelä, Miia R, Cerullo, Gabriella, Zhou, Miaomiao, Varriale, Simona, Gidijala, Loknath, Brás, Joana L A, Jütten, Peter, Piechot, Alexander, Verhaert, Raymond, Faraco, Vincenza, Hilden, Kristiina S, de Vries, Ronald P, Sub Molecular Plant Physiology, Molecular Plant Physiology, Dilokpimol, Adiphol, Mäkelä, Miia R, Cerullo, Gabriella, Zhou, Miaomiao, Varriale, Simona, Gidijala, Loknath, Brás, Joana L A, Jütten, Peter, Piechot, Alexander, Verhaert, Raymond, Faraco, Vincenza, Hilden, Kristiina S, and de Vries, Ronald P

Published
2018

13. Talaromyces borbonicus, sp. nov., a novel fungus from biodegraded Arundo donax with potential abilities in lignocellulose conversion.

Author

Varriale, Simona, Varriale, Simona, Houbraken, Jos, Granchi, Zoraide, Pepe, Olimpia, Cerullo, Gabriella, Ventorino, Valeria, Chin-A-Woeng, Thomas, Meijer, Martin, Riley, Robert, Grigoriev, Igor V, Henrissat, Bernard, de Vries, Ronald P, Faraco, Vincenza, Varriale, Simona, Varriale, Simona, Houbraken, Jos, Granchi, Zoraide, Pepe, Olimpia, Cerullo, Gabriella, Ventorino, Valeria, Chin-A-Woeng, Thomas, Meijer, Martin, Riley, Robert, Grigoriev, Igor V, Henrissat, Bernard, de Vries, Ronald P, and Faraco, Vincenza

Abstract

A novel fungal species able to synthesize enzymes with potential synergistic actions in lignocellulose conversion was isolated from the biomass of Arundo donax during biodegradation under natural conditions in the Gussone Park of the Royal Palace of Portici (Naples, Italy). In this work, this species was subjected to morphological and phylogenetic analyses. Sequencing of its genome was performed, resulting in 28 scaffolds that were assembled into 27.05 Mb containing 9744 predicted genes, among which 396 belong to carbohydrate-active enzyme (CAZyme)-encoding genes. Here we describe and illustrate this previously unknown species, which was named Talaromyces borbonicus, by a polyphasic approach combining phenotypic, physiological, and sequence data.

Published
2018

14. Draft Genome Sequence of Talaromyces adpressus

Author

Sub Molecular Microbiology, Sub Molecular Plant Physiology, Molecular Plant Physiology, Cerullo, Gabriella, Houbraken, Jos, Granchi, Zoraide, Pepe, Olimpia, Varriale, Simona, Ventorino, Valeria, Chin-A-Woeng, Thomas, Meijer, Martin, de Vries, Ronald P, Faraco, Vincenza, Sub Molecular Microbiology, Sub Molecular Plant Physiology, Molecular Plant Physiology, Cerullo, Gabriella, Houbraken, Jos, Granchi, Zoraide, Pepe, Olimpia, Varriale, Simona, Ventorino, Valeria, Chin-A-Woeng, Thomas, Meijer, Martin, de Vries, Ronald P, and Faraco, Vincenza

Published
2018

15. Fungal feruloyl esterases: Functional validation of genome mining based enzyme discovery including uncharacterized subfamilies

Author

Sub Molecular Plant Physiology, Molecular Plant Physiology, Dilokpimol, Adiphol, Mäkelä, Miia R, Varriale, Simona, Zhou, Miaomiao, Cerullo, Gabriella, Gidijala, Loknath, Hinkka, Harri, Brás, Joana L A, Jütten, Peter, Piechot, Alexander, Verhaert, Raymond, Hildén, Kristiina S, Faraco, Vincenza, de Vries, Ronald P, Sub Molecular Plant Physiology, Molecular Plant Physiology, Dilokpimol, Adiphol, Mäkelä, Miia R, Varriale, Simona, Zhou, Miaomiao, Cerullo, Gabriella, Gidijala, Loknath, Hinkka, Harri, Brás, Joana L A, Jütten, Peter, Piechot, Alexander, Verhaert, Raymond, Hildén, Kristiina S, Faraco, Vincenza, and de Vries, Ronald P

Published
2018

16. Fungal glucuronoyl esterases: Genome mining based enzyme discovery and biochemical characterization

Author

Sub Molecular Plant Physiology, Molecular Plant Physiology, Dilokpimol, Adiphol, Mäkelä, Miia R, Cerullo, Gabriella, Zhou, Miaomiao, Varriale, Simona, Gidijala, Loknath, Brás, Joana L A, Jütten, Peter, Piechot, Alexander, Verhaert, Raymond, Faraco, Vincenza, Hilden, Kristiina S, de Vries, Ronald P, Sub Molecular Plant Physiology, Molecular Plant Physiology, Dilokpimol, Adiphol, Mäkelä, Miia R, Cerullo, Gabriella, Zhou, Miaomiao, Varriale, Simona, Gidijala, Loknath, Brás, Joana L A, Jütten, Peter, Piechot, Alexander, Verhaert, Raymond, Faraco, Vincenza, Hilden, Kristiina S, and de Vries, Ronald P

Published
2018

17. The Synthetic Potential of Fungal Feruloyl Esterases: A Correlation with Current Classification Systems and Predicted Structural Properties

Author

Sub Molecular Plant Physiology, Molecular Plant Physiology, Antonopoulou, Io, Dilokpimol, Adiphol, Iancu, Laura, Makela, Miia R., Varriale, Simona, Cerullo, Gabriella, Huttner, Silvia, Uthoff, Stefan, Juetten, Peter, Piechot, Alexander, Steinbuechel, Alexander, Olsson, Lisbeth, Faraco, Vincenza, Hilden, Kristiina S., de Vries, Ronald P., Rova, Ulrika, Christakopoulos, Paul, Sub Molecular Plant Physiology, Molecular Plant Physiology, Antonopoulou, Io, Dilokpimol, Adiphol, Iancu, Laura, Makela, Miia R., Varriale, Simona, Cerullo, Gabriella, Huttner, Silvia, Uthoff, Stefan, Juetten, Peter, Piechot, Alexander, Steinbuechel, Alexander, Olsson, Lisbeth, Faraco, Vincenza, Hilden, Kristiina S., de Vries, Ronald P., Rova, Ulrika, and Christakopoulos, Paul

Published
2018

18. Fungal glucuronoyl esterases: Genome mining based enzyme discovery and biochemical characterization

Author

Sub Molecular Plant Physiology, Molecular Plant Physiology, Dilokpimol, Adiphol, Mäkelä, Miia R, Cerullo, Gabriella, Zhou, Miaomiao, Varriale, Simona, Gidijala, Loknath, Brás, Joana L A, Jütten, Peter, Piechot, Alexander, Verhaert, Raymond, Faraco, Vincenza, Hilden, Kristiina S, de Vries, Ronald P, Sub Molecular Plant Physiology, Molecular Plant Physiology, Dilokpimol, Adiphol, Mäkelä, Miia R, Cerullo, Gabriella, Zhou, Miaomiao, Varriale, Simona, Gidijala, Loknath, Brás, Joana L A, Jütten, Peter, Piechot, Alexander, Verhaert, Raymond, Faraco, Vincenza, Hilden, Kristiina S, and de Vries, Ronald P

Published
2018

19. Draft Genome Sequence of Talaromyces adpressus

Author

Sub Molecular Microbiology, Sub Molecular Plant Physiology, Molecular Plant Physiology, Cerullo, Gabriella, Houbraken, Jos, Granchi, Zoraide, Pepe, Olimpia, Varriale, Simona, Ventorino, Valeria, Chin-A-Woeng, Thomas, Meijer, Martin, de Vries, Ronald P, Faraco, Vincenza, Sub Molecular Microbiology, Sub Molecular Plant Physiology, Molecular Plant Physiology, Cerullo, Gabriella, Houbraken, Jos, Granchi, Zoraide, Pepe, Olimpia, Varriale, Simona, Ventorino, Valeria, Chin-A-Woeng, Thomas, Meijer, Martin, de Vries, Ronald P, and Faraco, Vincenza

Published
2018

20. Fungal feruloyl esterases: Functional validation of genome mining based enzyme discovery including uncharacterized subfamilies

Author

Sub Molecular Plant Physiology, Molecular Plant Physiology, Dilokpimol, Adiphol, Mäkelä, Miia R, Varriale, Simona, Zhou, Miaomiao, Cerullo, Gabriella, Gidijala, Loknath, Hinkka, Harri, Brás, Joana L A, Jütten, Peter, Piechot, Alexander, Verhaert, Raymond, Hildén, Kristiina S, Faraco, Vincenza, de Vries, Ronald P, Sub Molecular Plant Physiology, Molecular Plant Physiology, Dilokpimol, Adiphol, Mäkelä, Miia R, Varriale, Simona, Zhou, Miaomiao, Cerullo, Gabriella, Gidijala, Loknath, Hinkka, Harri, Brás, Joana L A, Jütten, Peter, Piechot, Alexander, Verhaert, Raymond, Hildén, Kristiina S, Faraco, Vincenza, and de Vries, Ronald P

Published
2018

21. The Synthetic Potential of Fungal Feruloyl Esterases: A Correlation with Current Classification Systems and Predicted Structural Properties

Author

Sub Molecular Plant Physiology, Molecular Plant Physiology, Antonopoulou, Io, Dilokpimol, Adiphol, Iancu, Laura, Makela, Miia R., Varriale, Simona, Cerullo, Gabriella, Huttner, Silvia, Uthoff, Stefan, Juetten, Peter, Piechot, Alexander, Steinbuechel, Alexander, Olsson, Lisbeth, Faraco, Vincenza, Hilden, Kristiina S., de Vries, Ronald P., Rova, Ulrika, Christakopoulos, Paul, Sub Molecular Plant Physiology, Molecular Plant Physiology, Antonopoulou, Io, Dilokpimol, Adiphol, Iancu, Laura, Makela, Miia R., Varriale, Simona, Cerullo, Gabriella, Huttner, Silvia, Uthoff, Stefan, Juetten, Peter, Piechot, Alexander, Steinbuechel, Alexander, Olsson, Lisbeth, Faraco, Vincenza, Hilden, Kristiina S., de Vries, Ronald P., Rova, Ulrika, and Christakopoulos, Paul

Published
2018

22. The Synthetic Potential of Fungal Feruloyl Esterases: A Correlation with Current Classification Systems and Predicted Structural Properties

Author

Antonopoulou, Io, Dilokpimol, Adiphol, Iancu, Laura, Makela, Miia R., Varriale, Simona, Cerullo, Gabriella, Huttner, Silvia, Uthoff, Stefan, Juetten, Peter, Piechot, Alexander, Steinbuechel, Alexander, Olsson, Lisbeth, Faraco, Vincenza, Hilden, Kristiina S., de Vries, Ronald P., Rova, Ulrika, Christakopoulos, Paul, Antonopoulou, Io, Dilokpimol, Adiphol, Iancu, Laura, Makela, Miia R., Varriale, Simona, Cerullo, Gabriella, Huttner, Silvia, Uthoff, Stefan, Juetten, Peter, Piechot, Alexander, Steinbuechel, Alexander, Olsson, Lisbeth, Faraco, Vincenza, Hilden, Kristiina S., de Vries, Ronald P., Rova, Ulrika, and Christakopoulos, Paul

Published
2018

23. Draft Genome Sequence of Talaromyces adpressus

Author

Cerullo, Gabriella, Houbraken, Jos, Granchi, Zoraide, Pepe, Olimpia, Varriale, Simona, Ventorino, Valeria, Chin-A-Woeng, Thomas, Meijer, Martin, de Vries, Ronald P., Faraco, Vincenza, Cerullo, Gabriella, Houbraken, Jos, Granchi, Zoraide, Pepe, Olimpia, Varriale, Simona, Ventorino, Valeria, Chin-A-Woeng, Thomas, Meijer, Martin, de Vries, Ronald P., and Faraco, Vincenza

Published
2018

24. Talaromyces borbonicus, sp. nov., a novel fungus from biodegraded Arundo donax with potential abilities in lignocellulose conversion.

Author

Varriale, Simona, Houbraken, J., Granchi, Zoraide, Pepe, Olimpia, Cerullo, Gabriella, Ventorino, Valeria, Chin-A-Woeng, Thomas, Meijer, M., Riley, Robert, Grigoriev, Igor V, Henrissat, Bernard, de Vries, R.P., Faraco, Vincenza, Varriale, Simona, Houbraken, J., Granchi, Zoraide, Pepe, Olimpia, Cerullo, Gabriella, Ventorino, Valeria, Chin-A-Woeng, Thomas, Meijer, M., Riley, Robert, Grigoriev, Igor V, Henrissat, Bernard, de Vries, R.P., and Faraco, Vincenza

Published
2018

25. Fungal feruloyl esterases: Functional validation of genome mining based enzyme discovery including uncharacterized subfamilies

Author

Dilokpimol, Adiphol, Makela, Miia R., Varriale, Simona, Zhou, Miaomiao, Cerullo, Gabriella, Gidijala, Loknath, Hinkka, Harri, Bras, Joana L. A., Juetten, Peter, Piechot, Alexander, Verhaert, Raymond, Hilden, Kristiina S., Faraco, Vincenza, de Vries, Ronald P., Dilokpimol, Adiphol, Makela, Miia R., Varriale, Simona, Zhou, Miaomiao, Cerullo, Gabriella, Gidijala, Loknath, Hinkka, Harri, Bras, Joana L. A., Juetten, Peter, Piechot, Alexander, Verhaert, Raymond, Hilden, Kristiina S., Faraco, Vincenza, and de Vries, Ronald P.

Abstract

Feruloyl esterases (FAEs) are a diverse group of enzymes that specifically catalyze the hydrolysis of ester bonds between a hydroxycinnamic (e.g. ferulic) acid and plant poly- or oligosaccharides. FAEs as auxiliary enzymes significantly assist xylanolytic and pectinolytic enzymes in gaining access to their site of action during biomass saccharification for biofuel and biochemical production. A limited number of FAEs have been functionally characterized compared to over 1000 putative fungal FAEs that were recently predicted by similarity-based genome mining, which divided phylogenetically into different subfamilies (SFs). In this study, 27 putative and six characterized FAEs from both ascomycete and basidiomycete fungi were selected and heterologously expressed in Pichia pastoris and the recombinant proteins biochemically characterized to validate the previous genome mining and phylogenetical grouping and to expand the information on activity of fungal FAEs. As a result, 20 enzymes were shown to possess FAE activity, being active towards pNP-ferulate and/or methyl hydroxycinnamate substrates, and covering 11 subfamilies. Most of the new FAEs showed activities comparable to those of previously characterized fungal FAEs.

Published
2018

26. Fungal glucuronoyl esterases: Genome mining based enzyme discovery and biochemical characterization

Author

Dilokpimol, Adiphol, Mäkelä, Miia R, Cerullo, Gabriella, Zhou, Miaomiao, Varriale, Simona, Gidijala, Loknath, Brás, Joana L A, Jütten, Peter, Piechot, Alexander, Verhaert, Raymond, Faraco, Vincenza, Hilden, Kristiina S., de Vries, Ronald P, Dilokpimol, Adiphol, Mäkelä, Miia R, Cerullo, Gabriella, Zhou, Miaomiao, Varriale, Simona, Gidijala, Loknath, Brás, Joana L A, Jütten, Peter, Piechot, Alexander, Verhaert, Raymond, Faraco, Vincenza, Hilden, Kristiina S., and de Vries, Ronald P

Abstract

4-O-Methyl-d-glucuronic acid (MeGlcA) is a side-residue of glucuronoarabinoxylan and can form ester linkages to lignin, contributing significantly to the strength and rigidity of the plant cell wall. Glucuronoyl esterases (4-O-methyl-glucuronoyl methylesterases, GEs) can cleave this ester bond, and therefore may play a significant role as auxiliary enzymes in biomass saccharification for the production of biofuels and biochemicals. GEs belong to a relatively new family of carbohydrate esterases (CE15) in the CAZy database (www.cazy.org), and so far around ten fungal GEs have been characterized. To explore additional GE enzymes, we used a genome mining strategy. BLAST analysis with characterized GEs against approximately 250 publicly accessible fungal genomes identified more than 150 putative fungal GEs, which were classified into eight phylogenetic sub-groups. To validate the genome mining strategy, 21 selected GEs from both ascomycete and basidiomycete fungi were heterologously produced in Pichia pastoris. Of these enzymes, 18 were active against benzyl d-glucuronate demonstrating the suitability of our genome mining strategy for enzyme discovery.

Published
2018

27. Water hyacinth a potential source for value addition: An overview

Author

Sindhu, Raveendran, Binod, Parameswaran, Pandey, Ashok, Madhavan, Aravind, Alphonsa, Jose Anju, Vivek, Narisetty, Gnansounou, Edgard, Castro, Eulogio, Faraco, Vincenza, Sindhu, Raveendran, Binod, Parameswaran, Pandey, Ashok, Madhavan, Aravind, Alphonsa, Jose Anju, Vivek, Narisetty, Gnansounou, Edgard, Castro, Eulogio, and Faraco, Vincenza

Abstract

Water hyacinth a fresh water aquatic plant is considered as a noxious weed in many parts of the world since it grows very fast and depletes nutrients and oxygen from water bodies adversely affecting the growth of both plants and animals. Hence conversion of this problematic weed to value added chemicals and fuels helps in the self-sustainability especially for developing countries. The present review discusses the various value added products and fuels which can be produced from water hyacinth, the recent research and developmental activities on the bioconversion of water hyacinth for the production of fuels and value added products as well as its possibilities and challenges in commercialization. (C) 2017 Elsevier Ltd. All rights reserved.

Published
2017
Full Text
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28. Water hyacinth a potential source for value addition: An overview

Author

Sindhu, Raveendran, Binod, Parameswaran, Pandey, Ashok, Madhavan, Aravind, Alphonsa, Jose Anju, Vivek, Narisetty, Gnansounou, Edgard, Castro, Eulogio, Faraco, Vincenza, Sindhu, Raveendran, Binod, Parameswaran, Pandey, Ashok, Madhavan, Aravind, Alphonsa, Jose Anju, Vivek, Narisetty, Gnansounou, Edgard, Castro, Eulogio, and Faraco, Vincenza

Abstract

Water hyacinth a fresh water aquatic plant is considered as a noxious weed in many parts of the world since it grows very fast and depletes nutrients and oxygen from water bodies adversely affecting the growth of both plants and animals. Hence conversion of this problematic weed to value added chemicals and fuels helps in the self-sustainability especially for developing countries. The present review discusses the various value added products and fuels which can be produced from water hyacinth, the recent research and developmental activities on the bioconversion of water hyacinth for the production of fuels and value added products as well as its possibilities and challenges in commercialization. (C) 2017 Elsevier Ltd. All rights reserved.

Published
2017
Full Text
View/download PDF

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