Control of cellular tRNA repertoires can drive specific programmes of translation to favour the maintenance of proliferative or differentiated phenotypes. tRNAiMet is the initiator methionine tRNA, responsible for recognising the start codon and initiating translation. We have investigated how increased expression of tRNAiMet can influence cell behaviour, using both immortalised cell lines in vitro and mouse models in vivo. Levels of tRNAiMet are increased in carcinoma-associated fibroblasts compared to normal fibroblasts. To understand the cellular effects of tRNAiMet overexpression in more detail we generated immortalised mouse embryonic fibroblasts (iMEFs) that overexpressed tRNAiMet (iMEF.tRNAiMet) or an empty vector as control (iMEF.Vector). Full characterisation of iMEF.Vector and iMEF.tRNAiMet cell lines showed that overexpression of tRNAiMet did not affect cell size, energy metabolism, cell spreading, rate of cellular protein synthesis or proliferation. Increased expression of tRNAiMet did, however, have marked effects on cell migration; with iMEF.tRNAiMet cells migrating approximately 1.5 fold faster than iMEF.Vector controls when assessing both directional and random migration. This tRNAiMet-driven increase in cell speed was dependent on the levels of phosphorylated eIF2α, indicating that fibroblast migration might be influenced by tRNAiMet in the ternary complex. Furthermore, the ability of tRNAiMet to increase cell migration depended on the ability of integrin α5β1 to bind its extracellular ligand fibronectin. However, despite the robust and reproducible role of both phospho-eIF2α and integrin α5β1 in this process, the way in which these are mechanistically linked to tRNAiMet levels is yet to be determined. To investigate whether increased stromal tRNAiMet expression may contribute to tumour progression, we utilised a mouse that expressed additional copies of the tRNAiMet gene (2+tRNAiMet mouse), and performed syngeneic allografts into these animals. Sub