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Ear biometrics has been found to be a good and reliable technique for human recognition. Initially ear biometrics could not gain popularity because there were doubts about its uniqueness. But, it started to gain momentum after a theory which came into existence and which said that it was very unlikely for any two years to be completely identical in all respects. The implemented methodology consists of steps such as pre-processing, feature extraction and matching based on the selected features. Our technique determines the extent to which these features support matching. The proposed work has been carried out on on a dataset containing 60 images for analyzing their features and matching of the source image with the dataset images. The results have been obtained on the basis of images correctly classified. The system accuracy telling us the extent to which matching could be performed on the basis of selected features.

Acute myeloid leukaemia (AML) is a heterogeneous disease characterized by transcriptional dysregulation that results in a block in differentiation and increased malignant self-renewal. Various epigenetic therapies aimed at reversing these hallmarks of AML have progressed into clinical trials, but most show only modest efficacy owing to an inability to effectively eradicate leukaemia stem cells (LSCs)1. Here, to specifically identify novel dependencies in LSCs, we screened a bespoke library of small hairpin RNAs that target chromatin regulators in a unique ex vivo mouse model of LSCs. We identify the MYST acetyltransferase HBO1 (also known as KAT7 or MYST2) and several known members of the HBO1 protein complex as critical regulators of LSC maintenance. Using CRISPR domain screening and quantitative mass spectrometry, we identified the histone acetyltransferase domain of HBO1 as being essential in the acetylation of histone H3 at K14. H3 acetylated at K14 (H3K14ac) facilitates the processivity of RNA polymerase II to maintain the high expression of key genes (including Hoxa9 and Hoxa10) that help to sustain the functional properties of LSCs. To leverage this dependency therapeutically, we developed a highly potent small-molecule inhibitor of HBO1 and demonstrate its mode of activity as a competitive analogue of acetyl-CoA. Inhibition of HBO1 phenocopied our genetic data and showed efficacy in a broad range of human cell lines and primary AML cells from patients. These biological, structural and chemical insights into a therapeutic target in AML will enable the clinical translation of these findings.

Long-term effect of nitrogen substitution (25 to 50%) through different organics, viz., FYM (farm yard ma-nure), GM (green manure) and WS (wheat straw) on crop yield, nutrition and physico-chemical properties of soil was studied under rice-wheat system. The data of long term experiment revealed that maximum grain yield of 46.83 qha-1 in wheat was obtained when 100% RDF applied through mineral fertilizers after 50% N of RDF being substituted with FYM in rice. Grain yield of wheat declined under control and sub-optimal fertilizer inputs (50% or 75% recommended fertilizer NPK), whereas positive yield increment was observed under treatments receiving organic supplements. The analysis of soil samples showed that soil pH reduced from initial value of 7.40 to 7.22, organic carbon build-up from 0.46% to 0.76%, available N from 194.00 to 225.95 kgha-1 available P2O5 from 23.60 to 49.54 kgha-1 and available K2O from 155.00 to 189.95 kgha-1 However, available Sulphur and DTPA-Zn increased from 7.74 to 14.41 kg ha-1 and 0.75 to 1.37 mg kg-1 respectively due to long-term (29 years) integrated nutrient management practices under rice-wheat system in alluvial soil. In conclusion, substitution of 50% and 25% N of RDF to rice through organics either FYM / Green manure / Wheat straw significantly increased the crop yield and nutrient uptake of wheat as well as build up the organic carbon, available N, P2O5, K2O, Sulphur, DTPA extractable Zn, Cu, Fe and Mn of post harvest soil after 29 years of the experiment.

The Hippo pathway has been identified as a key barrier for tumorigenesis, acting through downregulation of YAP/TAZ activity. Elevated YAP/TAZ activity has been documented in many human cancers. Ubiquitylation has been shown to play a key role in regulating YAP/TAZ activity through downregulation of a number of Hippo pathway components. Several ubiquitin ligase complexes have been implicated in this process, however, little is known about the deubiquitylating enzymes that counteract these activities to regulate YAP/TAZ. Here we identify the deubiquitylating enzyme USP9x as a regulator of YAP/TAZ activity. We demonstrate that USPx regulates ubiquitin-mediated turnover of the YAP inhibitor, Angiomotin. USP9x acts to deubiquitylate Angiomotin at lysine 496, resulting in stabilization of Angiomotin and lower YAP/TAZ activity. USP9x mRNA levels were reduced in several cancers. Clinically, USP9x mRNA levels were reduced in several cancers with low USPx expression correlating with poor prognosis in renal clear cell carcinoma. Our data indicate that USP9x may be a useful biomarker for renal clear cell carcinoma.

Colour in soils as well as other object is the visual perceptual property which is perceived by human eye. They are governed by spectrum of light corresponding to wavelength or reflected energy of the material. Developed model for soil acidity is based on visual interpretation, principal component and spectral enhancement techniques by using of the satellite image (IRS LISS III, 2014). In this context, red soil patch is much sensitive in red spectral band comparison to green and blue spectral bands and perceived as red tone by human eyes but same soil patch appears green in false colour composite (FCC) image of NIR (0.70-0.80?m), Red (0.60-0.70 ?m) and Green (0.50-0.60?m) bands. The maximum coverage of red soil patches having low pH < 6.5 (1:2.5) was recognized in 44.07 per cent of the total geographical area (3019.56 sq.km) under Banka district. Maximum red soil patches having their acidity were recognised in Katoria (18.56%), Chanan (15.15%), Bounsi (10.44%) and Banka (9.92%) blocks. Overall results indicated that variation of tone in different bands helps for the separation of red soil patches.

Activating mutations in the receptor tyrosine kinase FLT3 are frequently found in acute myelogenous leukemia patients and confer poor clinical prognosis. It is unclear how leukemic blasts escape cytokine control that regulates normal hematopoiesis. We have recently demonstrated that FLT3-internal tandem duplication (ITD), when localized to the biosynthetic compartment, aberrantly activates STAT5. Here, we show that one of the target genes induced by STAT5 is suppressor of cytokine signaling (SOCS)1 - a surprising finding for a known tumor suppressor. Although SOCS1 expression in murine bone marrow severely impaired cytokine-induced colony growth, it failed to inhibit FLT3-ITD-supported colony growth, indicating resistance of FLT3-ITD to SOCS1. In addition, SOCS1 coexpression did not affect FLT3-ITD-mediated signaling or proliferation. Importantly, SOCS1 coexpression inhibited interferon-αand interferon-γ signaling and protected FLT3-ITD hematopoietic cells from interferon-mediated growth inhibitory effects. In a murine bone marrow transplantation model, the coexpression of SOCS1 and FLT3-ITD significantly shortened the latency of a myeloproliferative disease compared with FLT3-ITD alone (P <.01). Mechanistically, SOCS proteins shield FLT3-ITD from external cytokine control, thereby promoting leukemogenesis. The data demonstrate that SOCS1 acts as a conditional oncogene, providing novel molecular insights into cytokine resistance in oncogenic transformation. Restoring cytokine control may provide a new way of therapeutic intervention.