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18 results on '"Carver, Akiko"'

1. The Transcription Factor Roc1 Is a Key Regulator of Cellulose Degradation in the Wood-Decaying Mushroom Schizophyllum commune

Author

Marian, Ioana M, Vonk, Peter Jan, Valdes, Ivan D, Barry, Kerrie, Bostock, Benedict, Carver, Akiko, Daum, Chris, Lerner, Harry, Lipzen, Anna, Park, Hongjae, Schuller, Margo B P, Tegelaar, Martin, Tritt, Andrew, Schmutz, Jeremy, Grimwood, Jane, Lugones, Luis G, Choi, In-Geol, Wösten, Han A B, Grigoriev, Igor V, Ohm, Robin A, Marian, Ioana M, Vonk, Peter Jan, Valdes, Ivan D, Barry, Kerrie, Bostock, Benedict, Carver, Akiko, Daum, Chris, Lerner, Harry, Lipzen, Anna, Park, Hongjae, Schuller, Margo B P, Tegelaar, Martin, Tritt, Andrew, Schmutz, Jeremy, Grimwood, Jane, Lugones, Luis G, Choi, In-Geol, Wösten, Han A B, Grigoriev, Igor V, and Ohm, Robin A

Abstract

Wood-decaying fungi of the class Agaricomycetes (phylum Basidiomycota) are saprotrophs that break down lignocellulose and play an important role in nutrient recycling. They secrete a wide range of extracellular plant cell wall degrading enzymes that break down cellulose, hemicellulose, and lignin, the main building blocks of plant biomass. Although the production of these enzymes is regulated mainly at the transcriptional level, no activating regulators have been identified in any wood-decaying fungus in the class Agaricomycetes. We studied the regulation of cellulase expression in the wood-decaying fungus Schizophyllum commune. Comparative genomics and transcriptomics on two wild isolates revealed a Zn2Cys6-type transcription factor gene (roc1) that was highly upregulated during growth on cellulose, compared to glucose. It is only conserved in the class Agaricomycetes. A roc1 knockout strain showed an inability to grow on medium with cellulose as sole carbon source, and growth on cellobiose and xylan (other components of wood) was inhibited. Growth on non-wood-related carbon sources was not inhibited. Cellulase gene expression and enzyme activity were reduced in the Droc1 strain. ChIP-Seq identified 1474 binding sites of the Roc1 transcription factor. Promoters of genes involved in lignocellulose degradation were enriched with these binding sites, especially those of LPMO (lytic polysaccharide monooxygenase) CAZymes, indicating that Roc1 directly regulates these genes. A conserved motif was identified as the binding site of Roc1, which was confirmed by a functional promoter analysis. Together, Roc1 is a key regulator of cellulose degradation and the first identified in wood-decaying fungi in the phylum Basidiomycota.

Published
2022

2. The Transcription Factor Roc1 Is a Key Regulator of Cellulose Degradation in the Wood-Decaying Mushroom Schizophyllum commune

Author

Molecular Microbiology, Sub Molecular Microbiology, Marian, Ioana M, Vonk, Peter Jan, Valdes, Ivan D, Barry, Kerrie, Bostock, Benedict, Carver, Akiko, Daum, Chris, Lerner, Harry, Lipzen, Anna, Park, Hongjae, Schuller, Margo B P, Tegelaar, Martin, Tritt, Andrew, Schmutz, Jeremy, Grimwood, Jane, Lugones, Luis G, Choi, In-Geol, Wösten, Han A B, Grigoriev, Igor V, Ohm, Robin A, Molecular Microbiology, Sub Molecular Microbiology, Marian, Ioana M, Vonk, Peter Jan, Valdes, Ivan D, Barry, Kerrie, Bostock, Benedict, Carver, Akiko, Daum, Chris, Lerner, Harry, Lipzen, Anna, Park, Hongjae, Schuller, Margo B P, Tegelaar, Martin, Tritt, Andrew, Schmutz, Jeremy, Grimwood, Jane, Lugones, Luis G, Choi, In-Geol, Wösten, Han A B, Grigoriev, Igor V, and Ohm, Robin A

Published
2022

3. Diversity of genomic adaptations to the post-fire environment in Pezizales fungi points to crosstalk between charcoal tolerance and sexual development.

Author

Steindorff, Andrei S, Steindorff, Andrei S, Seong, Kyungyong, Carver, Akiko, Calhoun, Sara, Fischer, Monika S, Stillman, Kyra, Liu, Haowen, Drula, Elodie, Henrissat, Bernard, Simpson, Hunter J, Schilling, Jonathan S, Lipzen, Anna, He, Guifen, Yan, Mi, Andreopoulos, Bill, Pangilinan, Jasmyn, LaButti, Kurt, Ng, Vivian, Traxler, Matthew, Bruns, Thomas D, Grigoriev, Igor V, Steindorff, Andrei S, Steindorff, Andrei S, Seong, Kyungyong, Carver, Akiko, Calhoun, Sara, Fischer, Monika S, Stillman, Kyra, Liu, Haowen, Drula, Elodie, Henrissat, Bernard, Simpson, Hunter J, Schilling, Jonathan S, Lipzen, Anna, He, Guifen, Yan, Mi, Andreopoulos, Bill, Pangilinan, Jasmyn, LaButti, Kurt, Ng, Vivian, Traxler, Matthew, Bruns, Thomas D, and Grigoriev, Igor V

Abstract

Wildfires drastically impact the soil environment, altering the soil organic matter, forming pyrolyzed compounds, and markedly reducing the diversity of microorganisms. Pyrophilous fungi, especially the species from the orders Pezizales and Agaricales, are fire-responsive fungal colonizers of post-fire soil that have historically been found fruiting on burned soil and thus may encode mechanisms of processing these compounds in their genomes. Pyrophilous fungi are diverse. In this work, we explored this diversity and sequenced six new genomes of pyrophilous Pezizales fungi isolated after the 2013 Rim Fire near Yosemite Park in California, USA: Pyronema domesticum, Pyronema omphalodes, Tricharina praecox, Geopyxis carbonaria, Morchella snyderi, and Peziza echinospora. A comparative genomics analysis revealed the enrichment of gene families involved in responses to stress and the degradation of pyrolyzed organic matter. In addition, we found that both protein sequence lengths and G + C content in the third base of codons (GC3) in pyrophilous fungi fall between those in mesophilic/nonpyrophilous and thermophilic fungi. A comparative transcriptome analysis of P. domesticum under two conditions - growing on charcoal, and during sexual development - identified modules of genes that are co-expressed in the charcoal and light-induced sexual development conditions. In addition, environmental sensors such as transcription factors STE12, LreA, LreB, VosA, and EsdC were upregulated in the charcoal condition. Taken together, these results highlight genomic adaptations of pyrophilous fungi and indicate a potential connection between charcoal tolerance and fruiting body formation in P. domesticum.

Published
2022

4. The Transcription Factor Roc1 Is a Key Regulator of Cellulose Degradation in the Wood-Decaying Mushroom Schizophyllum commune

Author

Molecular Microbiology, Sub Molecular Microbiology, Marian, Ioana M, Vonk, Peter Jan, Valdes, Ivan D, Barry, Kerrie, Bostock, Benedict, Carver, Akiko, Daum, Chris, Lerner, Harry, Lipzen, Anna, Park, Hongjae, Schuller, Margo B P, Tegelaar, Martin, Tritt, Andrew, Schmutz, Jeremy, Grimwood, Jane, Lugones, Luis G, Choi, In-Geol, Wösten, Han A B, Grigoriev, Igor V, Ohm, Robin A, Molecular Microbiology, Sub Molecular Microbiology, Marian, Ioana M, Vonk, Peter Jan, Valdes, Ivan D, Barry, Kerrie, Bostock, Benedict, Carver, Akiko, Daum, Chris, Lerner, Harry, Lipzen, Anna, Park, Hongjae, Schuller, Margo B P, Tegelaar, Martin, Tritt, Andrew, Schmutz, Jeremy, Grimwood, Jane, Lugones, Luis G, Choi, In-Geol, Wösten, Han A B, Grigoriev, Igor V, and Ohm, Robin A

Published
2022

5. The Transcription Factor Roc1 Is a Key Regulator of Cellulose Degradation in the Wood-Decaying Mushroom Schizophyllum commune

Author

Molecular Microbiology, Sub Molecular Microbiology, Marian, Ioana M, Vonk, Peter Jan, Valdes, Ivan D, Barry, Kerrie, Bostock, Benedict, Carver, Akiko, Daum, Chris, Lerner, Harry, Lipzen, Anna, Park, Hongjae, Schuller, Margo B P, Tegelaar, Martin, Tritt, Andrew, Schmutz, Jeremy, Grimwood, Jane, Lugones, Luis G, Choi, In-Geol, Wösten, Han A B, Grigoriev, Igor V, Ohm, Robin A, Molecular Microbiology, Sub Molecular Microbiology, Marian, Ioana M, Vonk, Peter Jan, Valdes, Ivan D, Barry, Kerrie, Bostock, Benedict, Carver, Akiko, Daum, Chris, Lerner, Harry, Lipzen, Anna, Park, Hongjae, Schuller, Margo B P, Tegelaar, Martin, Tritt, Andrew, Schmutz, Jeremy, Grimwood, Jane, Lugones, Luis G, Choi, In-Geol, Wösten, Han A B, Grigoriev, Igor V, and Ohm, Robin A

Published
2022

6. Diversity of genomic adaptations to the post-fire environment in Pezizales fungi points to crosstalk between charcoal tolerance and sexual development

Author

Steindorff, Andrei S., Seong, Kyungyong, Carver, Akiko, Calhoun, Sara, Fischer, Monika S., Stillman, Kyra, Liu, Haowen, Drula, Elodie, Henrissat, Bernard, Simpson, Hunter J., Schilling, Jonathan S., Lipzen, Anna, He, Guifen, Yan, Mi, Andreopoulos, Bill, Pangilinan, Jasmyn, LaButti, Kurt, Ng, Vivian, Traxler, Matthew, Bruns, Thomas D., Grigoriev, Igor, Steindorff, Andrei S., Seong, Kyungyong, Carver, Akiko, Calhoun, Sara, Fischer, Monika S., Stillman, Kyra, Liu, Haowen, Drula, Elodie, Henrissat, Bernard, Simpson, Hunter J., Schilling, Jonathan S., Lipzen, Anna, He, Guifen, Yan, Mi, Andreopoulos, Bill, Pangilinan, Jasmyn, LaButti, Kurt, Ng, Vivian, Traxler, Matthew, Bruns, Thomas D., and Grigoriev, Igor

Abstract

•Wildfires drastically impact the soil environment, altering the soil organic matter, forming pyrolyzed compounds, and markedly reducing the diversity of microorganisms. •Pyrophilous fungi, especially the species from the orders Pezizales and Agaricales, are fire-responsive fungal colonizers of post-fire soil that have historically been found fruiting on burned soil and thus may encode mechanisms of processing these compounds in their genomes. Pyrophilous fungi are diverse. In this work, we explored this diversity and sequenced six new genomes of pyrophilous Pezizales fungi isolated after the 2013 Rim Fire near Yosemite Park in California, USA: Pyronema domesticum, Pyronema omphalodes, Tricharina praecox, Geopyxis carbonaria, Morchella snyderi, and Peziza echinospora. •A comparative genomics analysis revealed the enrichment of gene families involved in responses to stress and the degradation of pyrolyzed organic matter. In addition, we found that both protein sequence lengths and G + C content in the third base of codons (GC3) in pyrophilous fungi fall between those in mesophilic/nonpyrophilous and thermophilic fungi. •A comparative transcriptome analysis of P. domesticum under two conditions - growing on charcoal, and during sexual development - identified modules of genes that are co-expressed in the charcoal and light-induced sexual development conditions. In addition, environmental sensors such as transcription factors STE12, LreA, LreB, VosA, and EsdC were upregulated in the charcoal condition. •Taken together, these results highlight genomic adaptations of pyrophilous fungi and indicate a potential connection between charcoal tolerance and fruiting body formation in P. domesticum.

Published
2022

7. Comparative genomics of pyrophilous fungi reveals a link between fire events and developmental genes.

Author

Steindorff, Andrei S, Steindorff, Andrei S, Carver, Akiko, Calhoun, Sara, Stillman, Kyra, Liu, Haowen, Lipzen, Anna, He, Guifen, Yan, Mi, Pangilinan, Jasmyn, LaButti, Kurt, Ng, Vivian, Bruns, Thomas D, Grigoriev, Igor V, Steindorff, Andrei S, Steindorff, Andrei S, Carver, Akiko, Calhoun, Sara, Stillman, Kyra, Liu, Haowen, Lipzen, Anna, He, Guifen, Yan, Mi, Pangilinan, Jasmyn, LaButti, Kurt, Ng, Vivian, Bruns, Thomas D, and Grigoriev, Igor V

Abstract

Forest fires generate a large amount of carbon that remains resident on the site as dead and partially 'pyrolysed' (i.e. burnt) material that has long residency times and constitutes a significant pool in fire-prone ecosystems. In addition, fire-induced hydrophobic soil layers, caused by condensation of pyrolysed waxes and lipids, increase post-fire erosion and can lead to long-term productivity losses. A small set of pyrophilous fungi dominate post-fire soils and are likely to be involved with the degradation of all these compounds, yet almost nothing is currently known about what these fungi do or the metabolic processes they employ. In this study, we sequenced and analysed genomes from fungi isolated after Rim fire near Yosemite National Park in 2013 and showed the enrichment/expansion of CAZymes and families known to be involved in fruiting body initiation when compared to other basidiomycete fungi. We found gene families potentially involved in the degradation of the hydrophobic layer and pyrolysed organic matter, such as hydrophobic surface binding proteins, laccases (AA1_1), xylanases (GH10, GH11), fatty acid desaturases and tannases. Thus, pyrophilous fungi are important actors to restate the soil's functional capabilities.

Published
2021

8. Comparative genomics of pyrophilous fungi reveals a link between fire events and developmental genes.

Author

Steindorff, Andrei S, Steindorff, Andrei S, Carver, Akiko, Calhoun, Sara, Stillman, Kyra, Liu, Haowen, Lipzen, Anna, He, Guifen, Yan, Mi, Pangilinan, Jasmyn, LaButti, Kurt, Ng, Vivian, Bruns, Thomas D, Grigoriev, Igor V, Steindorff, Andrei S, Steindorff, Andrei S, Carver, Akiko, Calhoun, Sara, Stillman, Kyra, Liu, Haowen, Lipzen, Anna, He, Guifen, Yan, Mi, Pangilinan, Jasmyn, LaButti, Kurt, Ng, Vivian, Bruns, Thomas D, and Grigoriev, Igor V

Abstract

Forest fires generate a large amount of carbon that remains resident on the site as dead and partially 'pyrolysed' (i.e. burnt) material that has long residency times and constitutes a significant pool in fire-prone ecosystems. In addition, fire-induced hydrophobic soil layers, caused by condensation of pyrolysed waxes and lipids, increase post-fire erosion and can lead to long-term productivity losses. A small set of pyrophilous fungi dominate post-fire soils and are likely to be involved with the degradation of all these compounds, yet almost nothing is currently known about what these fungi do or the metabolic processes they employ. In this study, we sequenced and analysed genomes from fungi isolated after Rim fire near Yosemite National Park in 2013 and showed the enrichment/expansion of CAZymes and families known to be involved in fruiting body initiation when compared to other basidiomycete fungi. We found gene families potentially involved in the degradation of the hydrophobic layer and pyrolysed organic matter, such as hydrophobic surface binding proteins, laccases (AA1_1), xylanases (GH10, GH11), fatty acid desaturases and tannases. Thus, pyrophilous fungi are important actors to restate the soil's functional capabilities.

Published
2021

9. Large-scale genome sequencing of mycorrhizal fungi provides insights into the early evolution of symbiotic traits

Author

Miyauchi, Shingo, Kiss, Enikő, Kuo, Alan, Drula, Elodie, Kohler, Annegret, Sánchez-García, Marisol, Morin, Emmanuelle, Andreopoulos, Bill, Barry, Kerrie W., Bonito, Gregory, Buée, Marc, Carver, Akiko, Chen, Cindy, Cichocki, Nicolas, Clum, Alicia, Culley, David, Crous, Pedro W., Fauchery, Laure, Girlanda, Mariangela, Hayes, Richard D., Kéri, Zsófia, LaButti, Kurt, Lipzen, Anna, Lombard, Vincent, Magnuson, Jon, Maillard, François, Murat, Claude, Nolan, Matt, Ohm, Robin A., Pangilinan, Jasmyn, Pereira, Maíra de Freitas, Perotto, Silvia, Peter, Martina, Pfister, Stephanie, Riley, Robert, Sitrit, Yaron, Stielow, J. Benjamin, Szöllősi, Gergely, Žifčáková, Lucia, Štursová, Martina, Spatafora, Joseph W., Tedersoo, Leho, Vaario, Lu Min, Yamada, Akiyoshi, Yan, Mi, Wang, Pengfei, Xu, Jianping, Bruns, Tom, Baldrian, Petr, Vilgalys, Rytas, Dunand, Christophe, Henrissat, Bernard, Grigoriev, Igor V., Hibbett, David, Nagy, László G., Martin, Francis M., Miyauchi, Shingo, Kiss, Enikő, Kuo, Alan, Drula, Elodie, Kohler, Annegret, Sánchez-García, Marisol, Morin, Emmanuelle, Andreopoulos, Bill, Barry, Kerrie W., Bonito, Gregory, Buée, Marc, Carver, Akiko, Chen, Cindy, Cichocki, Nicolas, Clum, Alicia, Culley, David, Crous, Pedro W., Fauchery, Laure, Girlanda, Mariangela, Hayes, Richard D., Kéri, Zsófia, LaButti, Kurt, Lipzen, Anna, Lombard, Vincent, Magnuson, Jon, Maillard, François, Murat, Claude, Nolan, Matt, Ohm, Robin A., Pangilinan, Jasmyn, Pereira, Maíra de Freitas, Perotto, Silvia, Peter, Martina, Pfister, Stephanie, Riley, Robert, Sitrit, Yaron, Stielow, J. Benjamin, Szöllősi, Gergely, Žifčáková, Lucia, Štursová, Martina, Spatafora, Joseph W., Tedersoo, Leho, Vaario, Lu Min, Yamada, Akiyoshi, Yan, Mi, Wang, Pengfei, Xu, Jianping, Bruns, Tom, Baldrian, Petr, Vilgalys, Rytas, Dunand, Christophe, Henrissat, Bernard, Grigoriev, Igor V., Hibbett, David, Nagy, László G., and Martin, Francis M.

Abstract

Mycorrhizal fungi are mutualists that play crucial roles in nutrient acquisition in terrestrial ecosystems. Mycorrhizal symbioses arose repeatedly across multiple lineages of Mucoromycotina, Ascomycota, and Basidiomycota. Considerable variation exists in the capacity of mycorrhizal fungi to acquire carbon from soil organic matter. Here, we present a combined analysis of 135 fungal genomes from 73 saprotrophic, endophytic and pathogenic species, and 62 mycorrhizal species, including 29 new mycorrhizal genomes. This study samples ecologically dominant fungal guilds for which there were previously no symbiotic genomes available, including ectomycorrhizal Russulales, Thelephorales and Cantharellales. Our analyses show that transitions from saprotrophy to symbiosis involve (1) widespread losses of degrading enzymes acting on lignin and cellulose, (2) co-option of genes present in saprotrophic ancestors to fulfill new symbiotic functions, (3) diversification of novel, lineage-specific symbiosis-induced genes, (4) proliferation of transposable elements and (5) divergent genetic innovations underlying the convergent origins of the ectomycorrhizal guild.

Published
2020

10. Large-scale genome sequencing of mycorrhizal fungi provides insights into the early evolution of symbiotic traits.

Author

Miyauchi, Shingo, Miyauchi, Shingo, Kiss, Enikő, Kuo, Alan, Drula, Elodie, Kohler, Annegret, Sánchez-García, Marisol, Morin, Emmanuelle, Andreopoulos, Bill, Barry, Kerrie W, Bonito, Gregory, Buée, Marc, Carver, Akiko, Chen, Cindy, Cichocki, Nicolas, Clum, Alicia, Culley, David, Crous, Pedro W, Fauchery, Laure, Girlanda, Mariangela, Hayes, Richard D, Kéri, Zsófia, LaButti, Kurt, Lipzen, Anna, Lombard, Vincent, Magnuson, Jon, Maillard, François, Murat, Claude, Nolan, Matt, Ohm, Robin A, Pangilinan, Jasmyn, Pereira, Maíra de Freitas, Perotto, Silvia, Peter, Martina, Pfister, Stephanie, Riley, Robert, Sitrit, Yaron, Stielow, J Benjamin, Szöllősi, Gergely, Žifčáková, Lucia, Štursová, Martina, Spatafora, Joseph W, Tedersoo, Leho, Vaario, Lu-Min, Yamada, Akiyoshi, Yan, Mi, Wang, Pengfei, Xu, Jianping, Bruns, Tom, Baldrian, Petr, Vilgalys, Rytas, Dunand, Christophe, Henrissat, Bernard, Grigoriev, Igor V, Hibbett, David, Nagy, László G, Martin, Francis M, Miyauchi, Shingo, Miyauchi, Shingo, Kiss, Enikő, Kuo, Alan, Drula, Elodie, Kohler, Annegret, Sánchez-García, Marisol, Morin, Emmanuelle, Andreopoulos, Bill, Barry, Kerrie W, Bonito, Gregory, Buée, Marc, Carver, Akiko, Chen, Cindy, Cichocki, Nicolas, Clum, Alicia, Culley, David, Crous, Pedro W, Fauchery, Laure, Girlanda, Mariangela, Hayes, Richard D, Kéri, Zsófia, LaButti, Kurt, Lipzen, Anna, Lombard, Vincent, Magnuson, Jon, Maillard, François, Murat, Claude, Nolan, Matt, Ohm, Robin A, Pangilinan, Jasmyn, Pereira, Maíra de Freitas, Perotto, Silvia, Peter, Martina, Pfister, Stephanie, Riley, Robert, Sitrit, Yaron, Stielow, J Benjamin, Szöllősi, Gergely, Žifčáková, Lucia, Štursová, Martina, Spatafora, Joseph W, Tedersoo, Leho, Vaario, Lu-Min, Yamada, Akiyoshi, Yan, Mi, Wang, Pengfei, Xu, Jianping, Bruns, Tom, Baldrian, Petr, Vilgalys, Rytas, Dunand, Christophe, Henrissat, Bernard, Grigoriev, Igor V, Hibbett, David, Nagy, László G, and Martin, Francis M

Abstract

Mycorrhizal fungi are mutualists that play crucial roles in nutrient acquisition in terrestrial ecosystems. Mycorrhizal symbioses arose repeatedly across multiple lineages of Mucoromycotina, Ascomycota, and Basidiomycota. Considerable variation exists in the capacity of mycorrhizal fungi to acquire carbon from soil organic matter. Here, we present a combined analysis of 135 fungal genomes from 73 saprotrophic, endophytic and pathogenic species, and 62 mycorrhizal species, including 29 new mycorrhizal genomes. This study samples ecologically dominant fungal guilds for which there were previously no symbiotic genomes available, including ectomycorrhizal Russulales, Thelephorales and Cantharellales. Our analyses show that transitions from saprotrophy to symbiosis involve (1) widespread losses of degrading enzymes acting on lignin and cellulose, (2) co-option of genes present in saprotrophic ancestors to fulfill new symbiotic functions, (3) diversification of novel, lineage-specific symbiosis-induced genes, (4) proliferation of transposable elements and (5) divergent genetic innovations underlying the convergent origins of the ectomycorrhizal guild.

Published
2020

11. Large-scale genome sequencing of mycorrhizal fungi provides insights into the early evolution of symbiotic traits

Author

Molecular Microbiology, Sub Molecular Microbiology, Miyauchi, Shingo, Kiss, Enikő, Kuo, Alan, Drula, Elodie, Kohler, Annegret, Sánchez-García, Marisol, Morin, Emmanuelle, Andreopoulos, Bill, Barry, Kerrie W., Bonito, Gregory, Buée, Marc, Carver, Akiko, Chen, Cindy, Cichocki, Nicolas, Clum, Alicia, Culley, David, Crous, Pedro W., Fauchery, Laure, Girlanda, Mariangela, Hayes, Richard D., Kéri, Zsófia, LaButti, Kurt, Lipzen, Anna, Lombard, Vincent, Magnuson, Jon, Maillard, François, Murat, Claude, Nolan, Matt, Ohm, Robin A., Pangilinan, Jasmyn, Pereira, Maíra de Freitas, Perotto, Silvia, Peter, Martina, Pfister, Stephanie, Riley, Robert, Sitrit, Yaron, Stielow, J. Benjamin, Szöllősi, Gergely, Žifčáková, Lucia, Štursová, Martina, Spatafora, Joseph W., Tedersoo, Leho, Vaario, Lu Min, Yamada, Akiyoshi, Yan, Mi, Wang, Pengfei, Xu, Jianping, Bruns, Tom, Baldrian, Petr, Vilgalys, Rytas, Dunand, Christophe, Henrissat, Bernard, Grigoriev, Igor V., Hibbett, David, Nagy, László G., Martin, Francis M., Molecular Microbiology, Sub Molecular Microbiology, Miyauchi, Shingo, Kiss, Enikő, Kuo, Alan, Drula, Elodie, Kohler, Annegret, Sánchez-García, Marisol, Morin, Emmanuelle, Andreopoulos, Bill, Barry, Kerrie W., Bonito, Gregory, Buée, Marc, Carver, Akiko, Chen, Cindy, Cichocki, Nicolas, Clum, Alicia, Culley, David, Crous, Pedro W., Fauchery, Laure, Girlanda, Mariangela, Hayes, Richard D., Kéri, Zsófia, LaButti, Kurt, Lipzen, Anna, Lombard, Vincent, Magnuson, Jon, Maillard, François, Murat, Claude, Nolan, Matt, Ohm, Robin A., Pangilinan, Jasmyn, Pereira, Maíra de Freitas, Perotto, Silvia, Peter, Martina, Pfister, Stephanie, Riley, Robert, Sitrit, Yaron, Stielow, J. Benjamin, Szöllősi, Gergely, Žifčáková, Lucia, Štursová, Martina, Spatafora, Joseph W., Tedersoo, Leho, Vaario, Lu Min, Yamada, Akiyoshi, Yan, Mi, Wang, Pengfei, Xu, Jianping, Bruns, Tom, Baldrian, Petr, Vilgalys, Rytas, Dunand, Christophe, Henrissat, Bernard, Grigoriev, Igor V., Hibbett, David, Nagy, László G., and Martin, Francis M.

Published
2020

12. Large-scale genome sequencing of mycorrhizal fungi provides insights into the early evolution of symbiotic traits

Author

Miyauchi, Shingo, Kiss, Enikő, Kuo, Alan, Drula, Elodie, Kohler, Annegret, Sánchez-García, Marisol, Morin, Emmanuelle, Andreopoulos, Bill, Barry, Kerrie W., Bonito, Gregory, Buée, Marc, Carver, Akiko, Chen, Cindy, Cichocki, Nicolas, Clum, Alicia, Culley, David, Crous, Pedro W., Fauchery, Laure, Girlanda, Mariangela, Hayes, Richard D., Kéri, Zsófia, LaButti, Kurt, Lipzen, Anna, Lombard, Vincent, Magnuson, Jon, Maillard, François, Murat, Claude, Nolan, Matt, Ohm, Robin A., Pangilinan, Jasmyn, Pereira, Maíra de Freitas, Perotto, Silvia, Peter, Martina, Pfister, Stephanie, Riley, Robert, Sitrit, Yaron, Stielow, J. Benjamin, Szöllősi, Gergely, Žifčáková, Lucia, Štursová, Martina, Spatafora, Joseph W., Tedersoo, Leho, Vaario, Lu-Min, Yamada, Akiyoshi, Yan, Mi, Wang, Pengfei, Xu, Jianping, Bruns, Tom, Baldrian, Petr, Vilgalys, Rytas, Dunand, Christophe, Henrissat, Bernard, Grigoriev, Igor V., Hibbett, David, Nagy, László G., Martin, Francis M., Miyauchi, Shingo, Kiss, Enikő, Kuo, Alan, Drula, Elodie, Kohler, Annegret, Sánchez-García, Marisol, Morin, Emmanuelle, Andreopoulos, Bill, Barry, Kerrie W., Bonito, Gregory, Buée, Marc, Carver, Akiko, Chen, Cindy, Cichocki, Nicolas, Clum, Alicia, Culley, David, Crous, Pedro W., Fauchery, Laure, Girlanda, Mariangela, Hayes, Richard D., Kéri, Zsófia, LaButti, Kurt, Lipzen, Anna, Lombard, Vincent, Magnuson, Jon, Maillard, François, Murat, Claude, Nolan, Matt, Ohm, Robin A., Pangilinan, Jasmyn, Pereira, Maíra de Freitas, Perotto, Silvia, Peter, Martina, Pfister, Stephanie, Riley, Robert, Sitrit, Yaron, Stielow, J. Benjamin, Szöllősi, Gergely, Žifčáková, Lucia, Štursová, Martina, Spatafora, Joseph W., Tedersoo, Leho, Vaario, Lu-Min, Yamada, Akiyoshi, Yan, Mi, Wang, Pengfei, Xu, Jianping, Bruns, Tom, Baldrian, Petr, Vilgalys, Rytas, Dunand, Christophe, Henrissat, Bernard, Grigoriev, Igor V., Hibbett, David, Nagy, László G., and Martin, Francis M.

Abstract

Mycorrhizal fungi are mutualists that play crucial roles in nutrient acquisition in terrestrial ecosystems. Mycorrhizal symbioses arose repeatedly across multiple lineages of Mucoromycotina, Ascomycota, and Basidiomycota. Considerable variation exists in the capacity of mycorrhizal fungi to acquire carbon from soil organic matter. Here, we present a combined analysis of 135 fungal genomes from 73 saprotrophic, endophytic and pathogenic species, and 62 mycorrhizal species, including 29 new mycorrhizal genomes. This study samples ecologically dominant fungal guilds for which there were previously no symbiotic genomes available, including ectomycorrhizal Russulales, Thelephorales and Cantharellales. Our analyses show that transitions from saprotrophy to symbiosis involve (1) widespread losses of degrading enzymes acting on lignin and cellulose, (2) co-option of genes present in saprotrophic ancestors to fulfill new symbiotic functions, (3) diversification of novel, lineage-specific symbiosis-induced genes, (4) proliferation of transposable elements and (5) divergent genetic innovations underlying the convergent origins of the ectomycorrhizal guild.

Published
2020

13. Large-scale genome sequencing of mycorrhizal fungi provides insights into the early evolution of symbiotic traits.

Author

Miyauchi, Shingo, Miyauchi, Shingo, Kiss, Enikő, Kuo, Alan, Drula, Elodie, Kohler, Annegret, Sánchez-García, Marisol, Morin, Emmanuelle, Andreopoulos, Bill, Barry, Kerrie W, Bonito, Gregory, Buée, Marc, Carver, Akiko, Chen, Cindy, Cichocki, Nicolas, Clum, Alicia, Culley, David, Crous, Pedro W, Fauchery, Laure, Girlanda, Mariangela, Hayes, Richard D, Kéri, Zsófia, LaButti, Kurt, Lipzen, Anna, Lombard, Vincent, Magnuson, Jon, Maillard, François, Murat, Claude, Nolan, Matt, Ohm, Robin A, Pangilinan, Jasmyn, Pereira, Maíra de Freitas, Perotto, Silvia, Peter, Martina, Pfister, Stephanie, Riley, Robert, Sitrit, Yaron, Stielow, J Benjamin, Szöllősi, Gergely, Žifčáková, Lucia, Štursová, Martina, Spatafora, Joseph W, Tedersoo, Leho, Vaario, Lu-Min, Yamada, Akiyoshi, Yan, Mi, Wang, Pengfei, Xu, Jianping, Bruns, Tom, Baldrian, Petr, Vilgalys, Rytas, Dunand, Christophe, Henrissat, Bernard, Grigoriev, Igor V, Hibbett, David, Nagy, László G, Martin, Francis M, Miyauchi, Shingo, Miyauchi, Shingo, Kiss, Enikő, Kuo, Alan, Drula, Elodie, Kohler, Annegret, Sánchez-García, Marisol, Morin, Emmanuelle, Andreopoulos, Bill, Barry, Kerrie W, Bonito, Gregory, Buée, Marc, Carver, Akiko, Chen, Cindy, Cichocki, Nicolas, Clum, Alicia, Culley, David, Crous, Pedro W, Fauchery, Laure, Girlanda, Mariangela, Hayes, Richard D, Kéri, Zsófia, LaButti, Kurt, Lipzen, Anna, Lombard, Vincent, Magnuson, Jon, Maillard, François, Murat, Claude, Nolan, Matt, Ohm, Robin A, Pangilinan, Jasmyn, Pereira, Maíra de Freitas, Perotto, Silvia, Peter, Martina, Pfister, Stephanie, Riley, Robert, Sitrit, Yaron, Stielow, J Benjamin, Szöllősi, Gergely, Žifčáková, Lucia, Štursová, Martina, Spatafora, Joseph W, Tedersoo, Leho, Vaario, Lu-Min, Yamada, Akiyoshi, Yan, Mi, Wang, Pengfei, Xu, Jianping, Bruns, Tom, Baldrian, Petr, Vilgalys, Rytas, Dunand, Christophe, Henrissat, Bernard, Grigoriev, Igor V, Hibbett, David, Nagy, László G, and Martin, Francis M

Abstract

Mycorrhizal fungi are mutualists that play crucial roles in nutrient acquisition in terrestrial ecosystems. Mycorrhizal symbioses arose repeatedly across multiple lineages of Mucoromycotina, Ascomycota, and Basidiomycota. Considerable variation exists in the capacity of mycorrhizal fungi to acquire carbon from soil organic matter. Here, we present a combined analysis of 135 fungal genomes from 73 saprotrophic, endophytic and pathogenic species, and 62 mycorrhizal species, including 29 new mycorrhizal genomes. This study samples ecologically dominant fungal guilds for which there were previously no symbiotic genomes available, including ectomycorrhizal Russulales, Thelephorales and Cantharellales. Our analyses show that transitions from saprotrophy to symbiosis involve (1) widespread losses of degrading enzymes acting on lignin and cellulose, (2) co-option of genes present in saprotrophic ancestors to fulfill new symbiotic functions, (3) diversification of novel, lineage-specific symbiosis-induced genes, (4) proliferation of transposable elements and (5) divergent genetic innovations underlying the convergent origins of the ectomycorrhizal guild.

Published
2020

14. Large-scale genome sequencing of mycorrhizal fungi provides insights into the early evolution of symbiotic traits

Author

Molecular Microbiology, Sub Molecular Microbiology, Miyauchi, Shingo, Kiss, Enikő, Kuo, Alan, Drula, Elodie, Kohler, Annegret, Sánchez-García, Marisol, Morin, Emmanuelle, Andreopoulos, Bill, Barry, Kerrie W., Bonito, Gregory, Buée, Marc, Carver, Akiko, Chen, Cindy, Cichocki, Nicolas, Clum, Alicia, Culley, David, Crous, Pedro W., Fauchery, Laure, Girlanda, Mariangela, Hayes, Richard D., Kéri, Zsófia, LaButti, Kurt, Lipzen, Anna, Lombard, Vincent, Magnuson, Jon, Maillard, François, Murat, Claude, Nolan, Matt, Ohm, Robin A., Pangilinan, Jasmyn, Pereira, Maíra de Freitas, Perotto, Silvia, Peter, Martina, Pfister, Stephanie, Riley, Robert, Sitrit, Yaron, Stielow, J. Benjamin, Szöllősi, Gergely, Žifčáková, Lucia, Štursová, Martina, Spatafora, Joseph W., Tedersoo, Leho, Vaario, Lu Min, Yamada, Akiyoshi, Yan, Mi, Wang, Pengfei, Xu, Jianping, Bruns, Tom, Baldrian, Petr, Vilgalys, Rytas, Dunand, Christophe, Henrissat, Bernard, Grigoriev, Igor V., Hibbett, David, Nagy, László G., Martin, Francis M., Molecular Microbiology, Sub Molecular Microbiology, Miyauchi, Shingo, Kiss, Enikő, Kuo, Alan, Drula, Elodie, Kohler, Annegret, Sánchez-García, Marisol, Morin, Emmanuelle, Andreopoulos, Bill, Barry, Kerrie W., Bonito, Gregory, Buée, Marc, Carver, Akiko, Chen, Cindy, Cichocki, Nicolas, Clum, Alicia, Culley, David, Crous, Pedro W., Fauchery, Laure, Girlanda, Mariangela, Hayes, Richard D., Kéri, Zsófia, LaButti, Kurt, Lipzen, Anna, Lombard, Vincent, Magnuson, Jon, Maillard, François, Murat, Claude, Nolan, Matt, Ohm, Robin A., Pangilinan, Jasmyn, Pereira, Maíra de Freitas, Perotto, Silvia, Peter, Martina, Pfister, Stephanie, Riley, Robert, Sitrit, Yaron, Stielow, J. Benjamin, Szöllősi, Gergely, Žifčáková, Lucia, Štursová, Martina, Spatafora, Joseph W., Tedersoo, Leho, Vaario, Lu Min, Yamada, Akiyoshi, Yan, Mi, Wang, Pengfei, Xu, Jianping, Bruns, Tom, Baldrian, Petr, Vilgalys, Rytas, Dunand, Christophe, Henrissat, Bernard, Grigoriev, Igor V., Hibbett, David, Nagy, László G., and Martin, Francis M.

Published
2020

15. Large-scale genome sequencing of mycorrhizal fungi provides insights into the early evolution of symbiotic traits

Author

Molecular Microbiology, Sub Molecular Microbiology, Miyauchi, Shingo, Kiss, Enikő, Kuo, Alan, Drula, Elodie, Kohler, Annegret, Sánchez-García, Marisol, Morin, Emmanuelle, Andreopoulos, Bill, Barry, Kerrie W., Bonito, Gregory, Buée, Marc, Carver, Akiko, Chen, Cindy, Cichocki, Nicolas, Clum, Alicia, Culley, David, Crous, Pedro W., Fauchery, Laure, Girlanda, Mariangela, Hayes, Richard D., Kéri, Zsófia, LaButti, Kurt, Lipzen, Anna, Lombard, Vincent, Magnuson, Jon, Maillard, François, Murat, Claude, Nolan, Matt, Ohm, Robin A., Pangilinan, Jasmyn, Pereira, Maíra de Freitas, Perotto, Silvia, Peter, Martina, Pfister, Stephanie, Riley, Robert, Sitrit, Yaron, Stielow, J. Benjamin, Szöllősi, Gergely, Žifčáková, Lucia, Štursová, Martina, Spatafora, Joseph W., Tedersoo, Leho, Vaario, Lu Min, Yamada, Akiyoshi, Yan, Mi, Wang, Pengfei, Xu, Jianping, Bruns, Tom, Baldrian, Petr, Vilgalys, Rytas, Dunand, Christophe, Henrissat, Bernard, Grigoriev, Igor V., Hibbett, David, Nagy, László G., Martin, Francis M., Molecular Microbiology, Sub Molecular Microbiology, Miyauchi, Shingo, Kiss, Enikő, Kuo, Alan, Drula, Elodie, Kohler, Annegret, Sánchez-García, Marisol, Morin, Emmanuelle, Andreopoulos, Bill, Barry, Kerrie W., Bonito, Gregory, Buée, Marc, Carver, Akiko, Chen, Cindy, Cichocki, Nicolas, Clum, Alicia, Culley, David, Crous, Pedro W., Fauchery, Laure, Girlanda, Mariangela, Hayes, Richard D., Kéri, Zsófia, LaButti, Kurt, Lipzen, Anna, Lombard, Vincent, Magnuson, Jon, Maillard, François, Murat, Claude, Nolan, Matt, Ohm, Robin A., Pangilinan, Jasmyn, Pereira, Maíra de Freitas, Perotto, Silvia, Peter, Martina, Pfister, Stephanie, Riley, Robert, Sitrit, Yaron, Stielow, J. Benjamin, Szöllősi, Gergely, Žifčáková, Lucia, Štursová, Martina, Spatafora, Joseph W., Tedersoo, Leho, Vaario, Lu Min, Yamada, Akiyoshi, Yan, Mi, Wang, Pengfei, Xu, Jianping, Bruns, Tom, Baldrian, Petr, Vilgalys, Rytas, Dunand, Christophe, Henrissat, Bernard, Grigoriev, Igor V., Hibbett, David, Nagy, László G., and Martin, Francis M.

Published
2020

16. Large-scale genome sequencing of mycorrhizal fungi provides insights into the early evolution of symbiotic traits

Author

Miyauchi, Shingo, Kiss, Enikő, Kuo, Alan, Drula, Elodie, Kohler, Annegret, Sánchez-García, Marisol, Morin, Emmanuelle, Andreopoulos, Bill, Barry, Kerrie W., Bonito, Gregory, Buée, Marc, Carver, Akiko, Chen, Cindy, Cichocki, Nicolas, Clum, Alicia, Culley, David, Crous, Pedro W., Fauchery, Laure, Girlanda, Mariangela, Hayes, Richard D., Kéri, Zsófia, LaButti, Kurt, Lipzen, Anna, Lombard, Vincent, Magnuson, Jon, Maillard, François, Murat, Claude, Nolan, Matt, Ohm, Robin A., Pangilinan, Jasmyn, Pereira, Maíra de Freitas, Perotto, Silvia, Peter, Martina, Pfister, Stephanie, Riley, Robert, Sitrit, Yaron, Stielow, J. Benjamin, Szöllősi, Gergely, Žifčáková, Lucia, Štursová, Martina, Spatafora, Joseph W., Tedersoo, Leho, Vaario, Lu-Min, Yamada, Akiyoshi, Yan, Mi, Wang, Pengfei, Xu, Jianping, Bruns, Tom, Baldrian, Petr, Vilgalys, Rytas, Dunand, Christophe, Henrissat, Bernard, Grigoriev, Igor V., Hibbett, David, Nagy, László G., Martin, Francis M., Miyauchi, Shingo, Kiss, Enikő, Kuo, Alan, Drula, Elodie, Kohler, Annegret, Sánchez-García, Marisol, Morin, Emmanuelle, Andreopoulos, Bill, Barry, Kerrie W., Bonito, Gregory, Buée, Marc, Carver, Akiko, Chen, Cindy, Cichocki, Nicolas, Clum, Alicia, Culley, David, Crous, Pedro W., Fauchery, Laure, Girlanda, Mariangela, Hayes, Richard D., Kéri, Zsófia, LaButti, Kurt, Lipzen, Anna, Lombard, Vincent, Magnuson, Jon, Maillard, François, Murat, Claude, Nolan, Matt, Ohm, Robin A., Pangilinan, Jasmyn, Pereira, Maíra de Freitas, Perotto, Silvia, Peter, Martina, Pfister, Stephanie, Riley, Robert, Sitrit, Yaron, Stielow, J. Benjamin, Szöllősi, Gergely, Žifčáková, Lucia, Štursová, Martina, Spatafora, Joseph W., Tedersoo, Leho, Vaario, Lu-Min, Yamada, Akiyoshi, Yan, Mi, Wang, Pengfei, Xu, Jianping, Bruns, Tom, Baldrian, Petr, Vilgalys, Rytas, Dunand, Christophe, Henrissat, Bernard, Grigoriev, Igor V., Hibbett, David, Nagy, László G., and Martin, Francis M.

Abstract

Mycorrhizal fungi are mutualists that play crucial roles in nutrient acquisition in terrestrial ecosystems. Mycorrhizal symbioses arose repeatedly across multiple lineages of Mucoromycotina, Ascomycota, and Basidiomycota. Considerable variation exists in the capacity of mycorrhizal fungi to acquire carbon from soil organic matter. Here, we present a combined analysis of 135 fungal genomes from 73 saprotrophic, endophytic and pathogenic species, and 62 mycorrhizal species, including 29 new mycorrhizal genomes. This study samples ecologically dominant fungal guilds for which there were previously no symbiotic genomes available, including ectomycorrhizal Russulales, Thelephorales and Cantharellales. Our analyses show that transitions from saprotrophy to symbiosis involve (1) widespread losses of degrading enzymes acting on lignin and cellulose, (2) co-option of genes present in saprotrophic ancestors to fulfill new symbiotic functions, (3) diversification of novel, lineage-specific symbiosis-induced genes, (4) proliferation of transposable elements and (5) divergent genetic innovations underlying the convergent origins of the ectomycorrhizal guild.

Published
2020

17. Functional and Comparative Genomics of Lignocellulose Degradation by Schizophyllum commune

Author

Ohm, Robin A., Ohm, Robin A., Lee, Hanbyul, Park, Hongjae, Brewer, Heather M., Carver, Akiko, Copeland, Alex, Grimwood, Jane, Lindquist, Erika, Lipzen, Anna, Martin, Joel, Purvine, Samuel O., Schackwitz, Wendy, Tegelaar, Martin, Tritt, Andrew, Baker, Scott, Choi, In-Geol, Lugones, Luis G., Wosten, Han A. B., Grigoriev, Igor V., Ohm, Robin A., Ohm, Robin A., Lee, Hanbyul, Park, Hongjae, Brewer, Heather M., Carver, Akiko, Copeland, Alex, Grimwood, Jane, Lindquist, Erika, Lipzen, Anna, Martin, Joel, Purvine, Samuel O., Schackwitz, Wendy, Tegelaar, Martin, Tritt, Andrew, Baker, Scott, Choi, In-Geol, Lugones, Luis G., Wosten, Han A. B., and Grigoriev, Igor V.

Abstract

The Basidiomycete fungus Schizophyllum commune is a wood-decaying fungus and is used as a model system to study lignocellulose degradation. Version 3.0 of the genome assembly filled 269 of 316 sequence gaps and added 680 kb of sequence. This new assembly was reannotated using RNAseq transcriptomics data, and this resulted in 3110 (24percent) more genes.Two additional S. commune strains with different wood-decaying properties were sequenced, from Tattone (France) and Loenen (The Netherlands). Sequence comparison shows remarkably high sequence diversity between the strains. The overall SNP rate of > 100 SNPs/kb is among the highest rates of within-species polymorphisms in Basidiomycetes. Some well-described proteins like hydrophobins and transcription factors have less than 70percent sequence identity among the strains. Some chromosomes are better conserved than others and in some cases large parts of chromosomes are missing from one or more strains.Gene expression on glucose, cellulose and wood was analyzed in two S. commune strains. Overall, gene expression correlated between the two strains, but there were some notable exceptions. Of particular interest are CAZymes (carbohydrate-active enzymes) that are regulated in different ways in the different strains.In both strains the transcription factor Fsp1 was strongly up-regulated during growth on cellulose and wood, when compared to glucose. Over-expression of Fsp1 using a constitutive promoter resulted in higher cellulose and xylose-degrading enzyme activity, which suggests that Fsp1 is involved in regulating CAZyme gene expression.Two CAZyme genes (of family GH61 and GH11) were shown to be strongly up-regulated during growth on cellulose, compared to glucose. Proteomics on the secreted proteins in the growth medium confirmed this. A promoter analysis revealed the shortest active promoters for these two genes, as well as putative transcription factor binding sites.

Published
2014

18. Functional and Comparative Genomics of Lignocellulose Degradation by Schizophyllum commune

Author

Ohm, Robin A., Ohm, Robin A., Lee, Hanbyul, Park, Hongjae, Brewer, Heather M., Carver, Akiko, Copeland, Alex, Grimwood, Jane, Lindquist, Erika, Lipzen, Anna, Martin, Joel, Purvine, Samuel O., Schackwitz, Wendy, Tegelaar, Martin, Tritt, Andrew, Baker, Scott, Choi, In-Geol, Lugones, Luis G., Wosten, Han A. B., Grigoriev, Igor V., Ohm, Robin A., Ohm, Robin A., Lee, Hanbyul, Park, Hongjae, Brewer, Heather M., Carver, Akiko, Copeland, Alex, Grimwood, Jane, Lindquist, Erika, Lipzen, Anna, Martin, Joel, Purvine, Samuel O., Schackwitz, Wendy, Tegelaar, Martin, Tritt, Andrew, Baker, Scott, Choi, In-Geol, Lugones, Luis G., Wosten, Han A. B., and Grigoriev, Igor V.

Abstract

The Basidiomycete fungus Schizophyllum commune is a wood-decaying fungus and is used as a model system to study lignocellulose degradation. Version 3.0 of the genome assembly filled 269 of 316 sequence gaps and added 680 kb of sequence. This new assembly was reannotated using RNAseq transcriptomics data, and this resulted in 3110 (24percent) more genes.Two additional S. commune strains with different wood-decaying properties were sequenced, from Tattone (France) and Loenen (The Netherlands). Sequence comparison shows remarkably high sequence diversity between the strains. The overall SNP rate of > 100 SNPs/kb is among the highest rates of within-species polymorphisms in Basidiomycetes. Some well-described proteins like hydrophobins and transcription factors have less than 70percent sequence identity among the strains. Some chromosomes are better conserved than others and in some cases large parts of chromosomes are missing from one or more strains.Gene expression on glucose, cellulose and wood was analyzed in two S. commune strains. Overall, gene expression correlated between the two strains, but there were some notable exceptions. Of particular interest are CAZymes (carbohydrate-active enzymes) that are regulated in different ways in the different strains.In both strains the transcription factor Fsp1 was strongly up-regulated during growth on cellulose and wood, when compared to glucose. Over-expression of Fsp1 using a constitutive promoter resulted in higher cellulose and xylose-degrading enzyme activity, which suggests that Fsp1 is involved in regulating CAZyme gene expression.Two CAZyme genes (of family GH61 and GH11) were shown to be strongly up-regulated during growth on cellulose, compared to glucose. Proteomics on the secreted proteins in the growth medium confirmed this. A promoter analysis revealed the shortest active promoters for these two genes, as well as putative transcription factor binding sites.

Published
2014

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