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This paper presents the study of Convolutional Neural Network based Image Classification. In this study, different convolutional neural network model is implemented for image classification. There are two application which is performed in this namely, emotion detection from facial emotion recognition system and covid-19 detection from lung CT-scan. In this research work, AlexNet and ResNet-50 convolutional neural network models are used for comparison and evaluation is done based on its training accuracy and testing accuracy, confusion matrix and Area under the curve (AUC) of ROC graph. All the experiments are performed on MATLAB software. In the First application of CNN model is implemented to detect emotions from facial expressions and SVM classifier is used for classification of each emotion among eight facial expressions namely, surprise, contempt, happiness, sadness, fear, anger, disgust, and neutral. The following proposed work is carried out on CK+ datasets to determine the recognition. After performing on both CNN models accuracy of both the models are compared. The result shows that ResNet-50 model achieved the best accuracy of 97.32% which is better than 90.55% which got on AlexNet model. In Second application of CNN model is to detect covid-19 from lung CT-scans. The highest performing model, the ResNet-50 on a SARS-CoV-2 CT-scan dataset achieved an accuracy of 95.72% which is maximum than 85.50% which achieved on AlexNet Model. Gradient-Weighted Class Activation Mapping (Grad-CAM) is also used to display infected area in the lungs. After performing this experiment, the final results shows that the ResNet-50 model, performs much better as compared to AlexNet model.

This paper presents the study of Convolutional Neural Network based Image Classification. In this study, different convolutional neural network model is implemented for image classification. There are two application which is performed in this namely, emotion detection from facial emotion recognition system and covid-19 detection from lung CT-scan. In this research work, AlexNet and ResNet-50 convolutional neural network models are used for comparison and evaluation is done based on its training accuracy and testing accuracy, confusion matrix and Area under the curve (AUC) of ROC graph. All the experiments are performed on MATLAB software. In the First application of CNN model is implemented to detect emotions from facial expressions and SVM classifier is used for classification of each emotion among eight facial expressions namely, surprise, contempt, happiness, sadness, fear, anger, disgust, and neutral. The following proposed work is carried out on CK+ datasets to determine the recognition. After performing on both CNN models accuracy of both the models are compared. The result shows that ResNet-50 model achieved the best accuracy of 97.32% which is better than 90.55% which got on AlexNet model. In Second application of CNN model is to detect covid-19 from lung CT-scans. The highest performing model, the ResNet-50 on a SARS-CoV-2 CT-scan dataset achieved an accuracy of 95.72% which is maximum than 85.50% which achieved on AlexNet Model. Gradient-Weighted Class Activation Mapping (Grad-CAM) is also used to display infected area in the lungs. After performing this experiment, the final results shows that the ResNet-50 model, performs much better as compared to AlexNet model.

This paper presents the study of Convolutional Neural Network based Image Classification. In this study, different convolutional neural network model is implemented for image classification. There are two application which is performed in this namely, emotion detection from facial emotion recognition system and covid-19 detection from lung CT-scan. In this research work, AlexNet and ResNet-50 convolutional neural network models are used for comparison and evaluation is done based on its training accuracy and testing accuracy, confusion matrix and Area under the curve (AUC) of ROC graph. All the experiments are performed on MATLAB software. In the First application of CNN model is implemented to detect emotions from facial expressions and SVM classifier is used for classification of each emotion among eight facial expressions namely, surprise, contempt, happiness, sadness, fear, anger, disgust, and neutral. The following proposed work is carried out on CK+ datasets to determine the recognition. After performing on both CNN models accuracy of both the models are compared. The result shows that ResNet-50 model achieved the best accuracy of 97.32% which is better than 90.55% which got on AlexNet model. In Second application of CNN model is to detect covid-19 from lung CT-scans. The highest performing model, the ResNet-50 on a SARS-CoV-2 CT-scan dataset achieved an accuracy of 95.72% which is maximum than 85.50% which achieved on AlexNet Model. Gradient-Weighted Class Activation Mapping (Grad-CAM) is also used to display infected area in the lungs. After performing this experiment, the final results shows that the ResNet-50 model, performs much better as compared to AlexNet model.

Objective. To investigate both muscular manifestations and CK levels in a large cohort of patients with COVID-19 infection and to determine whether hyperckemia is associated with morbidity and mortality. Methods. Data of 615 patients discharged from ASST Ovest Milanese (Milan, Lombardy, Italy) with final diagnosis of COVID-19 infection were retrospectively extracted from electronical medical records from 21 February to 1 May 2020. Patients were descriptively analyzed with respect to the following variables: sex, age, muscular manifestations (myalgia and/or arthralgia), fatigue, respiratory involvement (SARS pneumonia or respiratory failure) and history of falls. Association between patients' characteristics and CK levels was investigated. In addition, the proportion of patients who died following access to the ER was calculated. Finally, the effect of CK levels and other patients' features on mortality was estimated using a logistic regression model. Results. 176 (28.6%) patients had raised serum CK levels. CK levels were significantly associated with history of falls, male gender, SARS pneumonia, respiratory failure and in-hospital death. No correlation was found between hyperckemia and muscular manifestations. Conclusions. Our study provides preliminary evidence that hyperckemia is associated with respiratory failure and fatal outcome in patients with COVID-19 infection. In these patients, among other testing, CK dosage is recommended.

Introduction: Our goal was to assess the diagnostic utility and temporal kinetics of serum creatine kinase (CK) measurement as a predictor of acute kidney injury (AKI) in emergency department (ED) patients who present with possible substance-use related conditions.Methods: This was a retrospective chart review of ED patients with a urine drug screen (UDS) ordered and resulted between 2009–2013. Data was extracted electronically from EPIC Systems electronic health records, populated into a Microsoft Excel file, and includes demographics, chief complaint, vital signs, neuro-psychiatric physical examination findings, laboratory findings, psychiatric consult order time, ED medications given, orders, disposition and its time, and diagnosis.Results: Of 74,970 patients with an ED UDS, 22,101 (29%) had at least one CK measured. After inclusion and exclusion criteria, 2858 (13%) remained. Mean (standard deviation [SD]) age was 43.3 (12.5) years, 73% were male, 61% Black, 22% White, and 17% Hispanic. Mean (SD) ED length of stay was 10.4 (5.8) hours, and 56.7% were hospitalized. On average, CK was higher at 6-12 hours (p<0.001) and 12-18 hours (p=0.016) compared to 6 hours. CK was lower at 42-56 hours (p = 0.011), 72 hours (p<0.001), and over 72 hours (p<0.001), compared to 6 hours. Maximum CK was determined in those with >2 CK measures. We defined AKI risk as a creatinine of >1.4 milligrams per deciliter based on RIFLE criteria. AKI risk was calculated among those with at least two creatinine values in 522 patients. We identified five (1%) patients as having AKI risk. The odds of AKI risk were not associated with increase in CK over time.Conclusion: In 74,970 ED patients undergoing UDS testing for potential substance abuse, there was no identifiable CK level associated with AKI risk. In patients with possible substance-use conditions, CK continued to trend up even after six hours from door time and began to decrease after 42 hours. We found no value in repeated

Introduction: Our goal was to assess the diagnostic utility and temporal kinetics of serum creatine kinase (CK) measurement as a predictor of acute kidney injury (AKI) in emergency department (ED) patients who present with possible substance-use related conditions.Methods: This was a retrospective chart review of ED patients with a urine drug screen (UDS) ordered and resulted between 2009–2013. Data was extracted electronically from EPIC Systems electronic health records, populated into a Microsoft Excel file, and includes demographics, chief complaint, vital signs, neuro-psychiatric physical examination findings, laboratory findings, psychiatric consult order time, ED medications given, orders, disposition and its time, and diagnosis.Results: Of 74,970 patients with an ED UDS, 22,101 (29%) had at least one CK measured. After inclusion and exclusion criteria, 2858 (13%) remained. Mean (standard deviation [SD]) age was 43.3 (12.5) years, 73% were male, 61% Black, 22% White, and 17% Hispanic. Mean (SD) ED length of stay was 10.4 (5.8) hours, and 56.7% were hospitalized. On average, CK was higher at 6-12 hours (p<0.001) and 12-18 hours (p=0.016) compared to 6 hours. CK was lower at 42-56 hours (p = 0.011), 72 hours (p<0.001), and over 72 hours (p<0.001), compared to 6 hours. Maximum CK was determined in those with >2 CK measures. We defined AKI risk as a creatinine of >1.4 milligrams per deciliter based on RIFLE criteria. AKI risk was calculated among those with at least two creatinine values in 522 patients. We identified five (1%) patients as having AKI risk. The odds of AKI risk were not associated with increase in CK over time.Conclusion: In 74,970 ED patients undergoing UDS testing for potential substance abuse, there was no identifiable CK level associated with AKI risk. In patients with possible substance-use conditions, CK continued to trend up even after six hours from door time and began to decrease after 42 hours. We found no value in repeated

The authors report a case of fulminant myocarditis from an influenza A (H3N2) infection in a healthy individual who experienced cardiac arrest requiring extracorporeal membrane oxygenation (ECMO). The case highlights the management of complications arising from the use of ECMO including differential hypoxia and left ventricular overload requiring left ventricular venting. (Level of Difficulty: Beginner.).

Here we demonstrated that a single resistance exercise session causes muscle damage, delayed onset muscle soreness (DOMS), higher creatine kinase (CK) and lactate dehydrogenase (LDH) activity, and increased IL-6 concentration without changes in muscle strength. Sixteen healthy untrained subjects performed five exercises consisting of three sets of 10 maximum repetitions for each exercise and 1 minute rest period between sets and exercises. Blood samples were taken after 30 minutes, 24, 48 and 72 hours and before exercise. Muscular performance was assessed by maximum isometric strength (MIS) before, 24h, 48h and 72h exercise session. We have concluded that the single resistance exercise session, performed on this study, led to muscle damage and this variable cannot be evaluated through maximal isometric strength. Among those markers, CK was more sensitive to muscle damage. This information might be important for adequate recovery between training sessions.

This article discuss the algebra teaching knowledge of preservice elementary mathematics teachers in the context of CK and PCK as well as the relationship between them. The study was conducted with 101 preservice teachers sampled from a state university in Turkey. Rasch analysis was used to interpret the data. The results revealed that preservice teachers performed at mid-level for both CK and PCK tests. It was also found that there was significant correlation between the CK and PCK test scores. Weaknesses of the preservice teachers in terms of knowledge of the learner component of PCK, in comparison with presentation of content, were identified., Este estudio discute el conocimiento de la enseñanza del álgebra de maestros de matemáticas elementales en el contexto de CK y PCK, así como la relación entre ellos. El estudio se llevó a cabo con 101 futuros/as maestros/as, tomados de la universidad estatal de Turquía. Se utilizó el análisis de Rash para interpretar los datos. Los resultados revelaron que los maestros/as en formación se desempeñan en el nivel medio para las pruebas de CK y PCK. También se encontró que había una correlación significativa entre la puntuación de las pruebas CK y PCK. Se identificaron las debilidades de los futuros docentes en cuanto al conocimiento del del contenido y de los alumnos en el modelo PCK, en comparación con el componente “presentación del contenido.”

Here we demonstrated that a single resistance exercise session causes muscle damage, delayed onset muscle soreness (DOMS), higher creatine kinase (CK) and lactate dehydrogenase (LDH) activity, and increased IL-6 concentration without changes in muscle strength. Sixteen healthy untrained subjects performed five exercises consisting of three sets of 10 maximum repetitions for each exercise and 1 minute rest period between sets and exercises. Blood samples were taken after 30 minutes, 24, 48 and 72 hours and before exercise. Muscular performance was assessed by maximum isometric strength (MIS) before, 24h, 48h and 72h exercise session. We have concluded that the single resistance exercise session, performed on this study, led to muscle damage and this variable cannot be evaluated through maximal isometric strength. Among those markers, CK was more sensitive to muscle damage. This information might be important for adequate recovery between training sessions.

Document présenté au 4ème rencontre du Groupe Romand des Didacticiens des Sciences Naturelles, 22 juin 2017. HEP Lausanne.

Креатинфосфокиназа (КК) является маркером повреждения скелетных мышц. Цель исследования – изучение изменений биохимических и функциональных показателей, характерных для мужчин-спортсменов, в зависимости от активности креатинфосфокиназы. Материал и методы. Под наблюдением было 509 спортсменов-мужчин в возрасте от 15 до 20 лет, проходивших плановое обследование в Витебском областном диспансере спортивной медицины в 2012–2014 годах. Результаты и их обсуждение. Независимо от уровня активности КФК в сыворотке крови спортсменов были повышены содержание общего билирубина, активности креатинфосфокиназы, щелочной фосфатазы и величина отношения КФК/АсАТ, а также снижены содержание общего белка, альбумина, триглицеридов и активность альфа-амилазы. Эти изменения можно рассматривать как следствие систематических занятий спортом молодыми мужчинами (преимущественный выход КФК из саркомеров мышечных клеток) в условиях относительной недостаточности питания, не соответствующего интенсивности и силе систематических физических нагрузок (белок, альбумины, триглицериды). Установлено, что выход креатинфосфокиназы в кровь зависит от вида нагрузок: наименьшее повышение активности было отмечено при аэробном типе дыхания, уровень активности КФК при анаэробном типе дыхания соответствовал средним значениям активности фермента (контроль общий) и наибольшее повышение активности КФК было выявлено при смешанном типе дыхания (командные игровые виды спорта). Для оценки функционального состояния спортсменов было обосновано применение коэффициента КФК/ЩФ: верхняя граница нормы – 1,71; общий банк – 1,70; спортсмены без разряда – 0,81; первый юношеский разряд – 0,57; второй и третий взрослые разряды – 0,54; первый взрослый разряд – 1,14; кандидаты в мастера спорта – 1,80; мастера спорта – 2,18. Заключение. В статье обосновано применение отношения КФК/ЩФ для характеристики спортивной квалификации спортсменов. = Creatine phosphokinase (CK) is a marker of skeletal muscle damage. The purpose of the research

Purpose: This study assessed markers of muscle damage and training adaptations to eccentric-overload flywheel resistance exercise (RE) in men and women. Methods: Dynamic strength (1 RM), jump performance, maximal power at different percentages of 1 RM, and muscle mass in three different portions of the thigh were assessed in 16 men and 16 women before and after 6 weeks (15 sessions) of flywheel supine squat RE training. Plasma creatine kinase (CK) and lactate dehydrogenase (LDH) concentrations were measured before, 24, 48 and 72 h after the first and the last training session. Results: After training, increases in 1 RM were somewhat greater (interaction P < 0.001) in men (25 %) than in women (20 %). Squat and drop jump height and power performance at 50, 60, 70 and 80 % of 1 RM increased after training in both sexes (P < 0.05). Power improvement at 80 % of 1 RM was greater (interaction P < 0.02) in men than women. Muscle mass increased ~5 % in both groups (P < 0.05). CK increased in men after the first training session (P < 0.001), whereas the response in women was unaltered. In both sexes, LDH concentration was greater after the first training session compared with basal values (P < 0.05). After the last session, CK and LDH remained at baseline in both groups. Conclusions: These results suggest that although improvements in maximal strength and power at high loads may be slightly greater for men, eccentric-overload RE training induces comparable and favorable gains in strength, power, and muscle mass in both men and women. Equally important, it appears muscle damage does not interfere with the adaptations triggered by this training paradigm. © 2014 Springer-Verlag Berlin Heidelberg., Language of Original Document: English

Purpose: This study assessed markers of muscle damage and training adaptations to eccentric-overload flywheel resistance exercise (RE) in men and women. Methods: Dynamic strength (1 RM), jump performance, maximal power at different percentages of 1 RM, and muscle mass in three different portions of the thigh were assessed in 16 men and 16 women before and after 6 weeks (15 sessions) of flywheel supine squat RE training. Plasma creatine kinase (CK) and lactate dehydrogenase (LDH) concentrations were measured before, 24, 48 and 72 h after the first and the last training session. Results: After training, increases in 1 RM were somewhat greater (interaction P < 0.001) in men (25 %) than in women (20 %). Squat and drop jump height and power performance at 50, 60, 70 and 80 % of 1 RM increased after training in both sexes (P < 0.05). Power improvement at 80 % of 1 RM was greater (interaction P < 0.02) in men than women. Muscle mass increased ~5 % in both groups (P < 0.05). CK increased in men after the first training session (P < 0.001), whereas the response in women was unaltered. In both sexes, LDH concentration was greater after the first training session compared with basal values (P < 0.05). After the last session, CK and LDH remained at baseline in both groups. Conclusions: These results suggest that although improvements in maximal strength and power at high loads may be slightly greater for men, eccentric-overload RE training induces comparable and favorable gains in strength, power, and muscle mass in both men and women. Equally important, it appears muscle damage does not interfere with the adaptations triggered by this training paradigm. © 2014 Springer-Verlag Berlin Heidelberg., Language of Original Document: English

Purpose: This study assessed markers of muscle damage and training adaptations to eccentric-overload flywheel resistance exercise (RE) in men and women. Methods: Dynamic strength (1 RM), jump performance, maximal power at different percentages of 1 RM, and muscle mass in three different portions of the thigh were assessed in 16 men and 16 women before and after 6 weeks (15 sessions) of flywheel supine squat RE training. Plasma creatine kinase (CK) and lactate dehydrogenase (LDH) concentrations were measured before, 24, 48 and 72 h after the first and the last training session. Results: After training, increases in 1 RM were somewhat greater (interaction P < 0.001) in men (25 %) than in women (20 %). Squat and drop jump height and power performance at 50, 60, 70 and 80 % of 1 RM increased after training in both sexes (P < 0.05). Power improvement at 80 % of 1 RM was greater (interaction P < 0.02) in men than women. Muscle mass increased ~5 % in both groups (P < 0.05). CK increased in men after the first training session (P < 0.001), whereas the response in women was unaltered. In both sexes, LDH concentration was greater after the first training session compared with basal values (P < 0.05). After the last session, CK and LDH remained at baseline in both groups. Conclusions: These results suggest that although improvements in maximal strength and power at high loads may be slightly greater for men, eccentric-overload RE training induces comparable and favorable gains in strength, power, and muscle mass in both men and women. Equally important, it appears muscle damage does not interfere with the adaptations triggered by this training paradigm. © 2014 Springer-Verlag Berlin Heidelberg., Language of Original Document: English

Purpose: This study assessed markers of muscle damage and training adaptations to eccentric-overload flywheel resistance exercise (RE) in men and women. Methods: Dynamic strength (1 RM), jump performance, maximal power at different percentages of 1 RM, and muscle mass in three different portions of the thigh were assessed in 16 men and 16 women before and after 6 weeks (15 sessions) of flywheel supine squat RE training. Plasma creatine kinase (CK) and lactate dehydrogenase (LDH) concentrations were measured before, 24, 48 and 72 h after the first and the last training session. Results: After training, increases in 1 RM were somewhat greater (interaction P < 0.001) in men (25 %) than in women (20 %). Squat and drop jump height and power performance at 50, 60, 70 and 80 % of 1 RM increased after training in both sexes (P < 0.05). Power improvement at 80 % of 1 RM was greater (interaction P < 0.02) in men than women. Muscle mass increased ~5 % in both groups (P < 0.05). CK increased in men after the first training session (P < 0.001), whereas the response in women was unaltered. In both sexes, LDH concentration was greater after the first training session compared with basal values (P < 0.05). After the last session, CK and LDH remained at baseline in both groups. Conclusions: These results suggest that although improvements in maximal strength and power at high loads may be slightly greater for men, eccentric-overload RE training induces comparable and favorable gains in strength, power, and muscle mass in both men and women. Equally important, it appears muscle damage does not interfere with the adaptations triggered by this training paradigm. © 2014 Springer-Verlag Berlin Heidelberg., Language of Original Document: English

Purpose: This study assessed markers of muscle damage and training adaptations to eccentric-overload flywheel resistance exercise (RE) in men and women. Methods: Dynamic strength (1 RM), jump performance, maximal power at different percentages of 1 RM, and muscle mass in three different portions of the thigh were assessed in 16 men and 16 women before and after 6 weeks (15 sessions) of flywheel supine squat RE training. Plasma creatine kinase (CK) and lactate dehydrogenase (LDH) concentrations were measured before, 24, 48 and 72 h after the first and the last training session. Results: After training, increases in 1 RM were somewhat greater (interaction P < 0.001) in men (25 %) than in women (20 %). Squat and drop jump height and power performance at 50, 60, 70 and 80 % of 1 RM increased after training in both sexes (P < 0.05). Power improvement at 80 % of 1 RM was greater (interaction P < 0.02) in men than women. Muscle mass increased ~5 % in both groups (P < 0.05). CK increased in men after the first training session (P < 0.001), whereas the response in women was unaltered. In both sexes, LDH concentration was greater after the first training session compared with basal values (P < 0.05). After the last session, CK and LDH remained at baseline in both groups. Conclusions: These results suggest that although improvements in maximal strength and power at high loads may be slightly greater for men, eccentric-overload RE training induces comparable and favorable gains in strength, power, and muscle mass in both men and women. Equally important, it appears muscle damage does not interfere with the adaptations triggered by this training paradigm. © 2014 Springer-Verlag Berlin Heidelberg., Language of Original Document: English

Prostasomes are extracellularly occurring submicron, membrane-surrounded organelles produced by the epithelial cells of the prostate and present in semen after secretion. Even dedifferentiated prostate cancer cells have preserved their ability to produce and export prostasomes to the extracellular space. The precise physiological role of prostasomes is not known, although some of their properties assign them to important physiological and patho-physiological functions that could be exploited in prostate cancer growth and development. In this review, some new properties of seminal and malignant cell line (DU145, PC-3 and LNCaP) prostasomes will be discussed. There are typical differences in the expressions and activities of prostasomal CD59, ATPase, protein kinases and tissue factor (TF) as well as in the transfer of prostasomal CD59 to CD59-deficient erythrocytes (rabbit and human PNH erythrocytes). CD59, protein kinases and TF exhibit characteristic patterns of overexpression by malignant cell prostasomes. A high ATPase activity is recognized on seminal prostasomes with minimal activity on malignant cell prostasomes resulting in more residual ATP available for phosphorylation reactions. Several proteins are phosphorylated by prostasomal protein kinases, namely, complement component C3, fibrinogen, vitronectin and E-cadherin. Furthermore, TF is identified as the main endogenous phosphorylation substrate on prostasomes. In addition, prothrombotic effects of prostasomes are demonstrated. DU145 and PC-3 cell-derived prostasomes exert a higher clotting effect on whole blood and plasma compared to LNCaP cell-derived and seminal prostasomes. In conclusion, malignant cell prostasomes show an increased ability to interact with the biological system in favor of prostate cancer cell promotion and survival. The roles played by prostasomes in this context may improve the understanding of the mechanisms that help the prostate cancer cells to avoid the complement attack (CD59 tra

Prostasomes are extracellularly occurring submicron, membrane-surrounded organelles produced by the epithelial cells of the prostate and present in semen after secretion. Even dedifferentiated prostate cancer cells have preserved their ability to produce and export prostasomes to the extracellular space. The precise physiological role of prostasomes is not known, although some of their properties assign them to important physiological and patho-physiological functions that could be exploited in prostate cancer growth and development. In this review, some new properties of seminal and malignant cell line (DU145, PC-3 and LNCaP) prostasomes will be discussed. There are typical differences in the expressions and activities of prostasomal CD59, ATPase, protein kinases and tissue factor (TF) as well as in the transfer of prostasomal CD59 to CD59-deficient erythrocytes (rabbit and human PNH erythrocytes). CD59, protein kinases and TF exhibit characteristic patterns of overexpression by malignant cell prostasomes. A high ATPase activity is recognized on seminal prostasomes with minimal activity on malignant cell prostasomes resulting in more residual ATP available for phosphorylation reactions. Several proteins are phosphorylated by prostasomal protein kinases, namely, complement component C3, fibrinogen, vitronectin and E-cadherin. Furthermore, TF is identified as the main endogenous phosphorylation substrate on prostasomes. In addition, prothrombotic effects of prostasomes are demonstrated. DU145 and PC-3 cell-derived prostasomes exert a higher clotting effect on whole blood and plasma compared to LNCaP cell-derived and seminal prostasomes. In conclusion, malignant cell prostasomes show an increased ability to interact with the biological system in favor of prostate cancer cell promotion and survival. The roles played by prostasomes in this context may improve the understanding of the mechanisms that help the prostate cancer cells to avoid the complement attack (CD59 tra

Strength and flexibility, important components of a training and their maximal values are obtained through specific tests. However, little information about the damage effect in a skeletal muscle is known. The aim was to verify a serum CK changes 24 h after a lengthening and static flexibilizing routine and a maximal dynamic strength tests. The sample, was by 14 subjects (man and women, 28 ± 6 yr.), control group (CG N = 7) and experimental group (EG N = 4) that was submitted a lengthening routine (EG-LG), a static flexibilizing routine (EG-FLEX) and a 1-RM test (EG-1-RM). The anthropometrics were obtained by digital scale with stadiometer. The blood samples were obtained using the IFCC method with reference values 26-155 U/L. The De Lorme and Watkins technique was used to access maximal dynamic strength through bench press and leg press. The static flexibilizing routine consisted in three 20 seconds sets until the point of maximal discomfort. The lengthening was done in normal movement amplitude during 6 seconds. In analysis inter groups was a significant difference (p < .05) in the values of GE-1RM (D = 118,7 U/L, p= .02) when compared to the GC. We concluded that only maximal strength dynamic test was capable to raise the CK serum levels 24 h after exercise, La fuerza y flexibilidad, muy importantes en entrenamiento, obtienen sus valores máximos a través de test específicos. Se sabe poco sobre sus efectos perjudiciales en el aparato músculo tendinoso. El objetivo fue verificar las modificaciones séricas de CK 24h después de estiramientos, flexibilidad estática y test 1- RM. Muestra de 14 individuos, ambos sexos (28 ± 6 años), grupo control (GC N = 7) y grupo experimental (GE N = 7), el GE fue sometido a una rutina de estiramientos (GE-AL), flexibilidad estática (GE-FLEX) y test 1-RM (GE-1-RM). La antropometría con una balanza digital con estadiómetro. Las tomas de sangre, obtenidas con jeringuillas desechables y depositadas en recipientes cerrados y enviados al laboratorio. Se utilizó la técnica De Lorme y Watkins en ejercicios Supino Horizontal y Leg Press para determinar carga de 1-RM. Se realizaron 3 series de 20s de flexibilidad estática y 3 de 6 segundos de estiramiento asistido. La estadística fue el Test de Friedman con post hoc de Tukey. En el análisis intergrupos se encontraron diferencias significativas (p < .05) en valores del GE-1RM (D= 118,7 U/L, p= .02) comparando con GC. Como conclusión, los ejercicios dinámicos de fuerza máxima fueron capaces de aumentar los niveles de CK post-ejercicio.

Prostasomes are extracellularly occurring submicron, membrane-surrounded organelles produced by the epithelial cells of the prostate and present in semen after secretion. Even dedifferentiated prostate cancer cells have preserved their ability to produce and export prostasomes to the extracellular space. The precise physiological role of prostasomes is not known, although some of their properties assign them to important physiological and patho-physiological functions that could be exploited in prostate cancer growth and development. In this review, some new properties of seminal and malignant cell line (DU145, PC-3 and LNCaP) prostasomes will be discussed. There are typical differences in the expressions and activities of prostasomal CD59, ATPase, protein kinases and tissue factor (TF) as well as in the transfer of prostasomal CD59 to CD59-deficient erythrocytes (rabbit and human PNH erythrocytes). CD59, protein kinases and TF exhibit characteristic patterns of overexpression by malignant cell prostasomes. A high ATPase activity is recognized on seminal prostasomes with minimal activity on malignant cell prostasomes resulting in more residual ATP available for phosphorylation reactions. Several proteins are phosphorylated by prostasomal protein kinases, namely, complement component C3, fibrinogen, vitronectin and E-cadherin. Furthermore, TF is identified as the main endogenous phosphorylation substrate on prostasomes. In addition, prothrombotic effects of prostasomes are demonstrated. DU145 and PC-3 cell-derived prostasomes exert a higher clotting effect on whole blood and plasma compared to LNCaP cell-derived and seminal prostasomes. In conclusion, malignant cell prostasomes show an increased ability to interact with the biological system in favor of prostate cancer cell promotion and survival. The roles played by prostasomes in this context may improve the understanding of the mechanisms that help the prostate cancer cells to avoid the complement attack (CD59 tra

Prostasomes are extracellularly occurring submicron, membrane-surrounded organelles produced by the epithelial cells of the prostate and present in semen after secretion. Even dedifferentiated prostate cancer cells have preserved their ability to produce and export prostasomes to the extracellular space. The precise physiological role of prostasomes is not known, although some of their properties assign them to important physiological and patho-physiological functions that could be exploited in prostate cancer growth and development. In this review, some new properties of seminal and malignant cell line (DU145, PC-3 and LNCaP) prostasomes will be discussed. There are typical differences in the expressions and activities of prostasomal CD59, ATPase, protein kinases and tissue factor (TF) as well as in the transfer of prostasomal CD59 to CD59-deficient erythrocytes (rabbit and human PNH erythrocytes). CD59, protein kinases and TF exhibit characteristic patterns of overexpression by malignant cell prostasomes. A high ATPase activity is recognized on seminal prostasomes with minimal activity on malignant cell prostasomes resulting in more residual ATP available for phosphorylation reactions. Several proteins are phosphorylated by prostasomal protein kinases, namely, complement component C3, fibrinogen, vitronectin and E-cadherin. Furthermore, TF is identified as the main endogenous phosphorylation substrate on prostasomes. In addition, prothrombotic effects of prostasomes are demonstrated. DU145 and PC-3 cell-derived prostasomes exert a higher clotting effect on whole blood and plasma compared to LNCaP cell-derived and seminal prostasomes. In conclusion, malignant cell prostasomes show an increased ability to interact with the biological system in favor of prostate cancer cell promotion and survival. The roles played by prostasomes in this context may improve the understanding of the mechanisms that help the prostate cancer cells to avoid the complement attack (CD59 tra

Genetic algorithms (GAs) have been used in the clustering subject. Also, a clustering ensemble as one acceptable clustering method combines the results of multiple clustering methods on a given dataset and creates final clustering on the dataset. In this paper, genetic algorithm base on clustering ensemble (GACE) is introduced for finding optimal clusters. The most important property of our method is the ability to extract the number of clusters. With this ability, the need for data examination is removed, and then solving related problems will not be time consuming. GACE is applied to eight series of databases. Experimental results were compared with other four clustering methods. Data envelopment analysis (DEA) is used to compare methods. The results of DEA indicate that GACE is the best method. The four methods are co-association function and average link (CAL), co-association function and K-means (CK), hypergraph-partitioning algorithm (HGPA) and cluster-based similarity partitioning (CSPA).

Prostasomes are extracellularly occurring submicron, membrane-surrounded organelles produced by the epithelial cells of the prostate and present in semen after secretion. Even dedifferentiated prostate cancer cells have preserved their ability to produce and export prostasomes to the extracellular space. The precise physiological role of prostasomes is not known, although some of their properties assign them to important physiological and patho-physiological functions that could be exploited in prostate cancer growth and development. In this review, some new properties of seminal and malignant cell line (DU145, PC-3 and LNCaP) prostasomes will be discussed. There are typical differences in the expressions and activities of prostasomal CD59, ATPase, protein kinases and tissue factor (TF) as well as in the transfer of prostasomal CD59 to CD59-deficient erythrocytes (rabbit and human PNH erythrocytes). CD59, protein kinases and TF exhibit characteristic patterns of overexpression by malignant cell prostasomes. A high ATPase activity is recognized on seminal prostasomes with minimal activity on malignant cell prostasomes resulting in more residual ATP available for phosphorylation reactions. Several proteins are phosphorylated by prostasomal protein kinases, namely, complement component C3, fibrinogen, vitronectin and E-cadherin. Furthermore, TF is identified as the main endogenous phosphorylation substrate on prostasomes. In addition, prothrombotic effects of prostasomes are demonstrated. DU145 and PC-3 cell-derived prostasomes exert a higher clotting effect on whole blood and plasma compared to LNCaP cell-derived and seminal prostasomes. In conclusion, malignant cell prostasomes show an increased ability to interact with the biological system in favor of prostate cancer cell promotion and survival. The roles played by prostasomes in this context may improve the understanding of the mechanisms that help the prostate cancer cells to avoid the complement attack (CD59 tra

Preliminary investigation results in physiological traits and changes due to exercise (horse jumping competition) in equines in Colombia are presented. 24 horses were selected (Silla argentina, PSI and crossbred) at the National Army Cavalry school, where cardiac and respiratory rate, creatinkinase (CK), lactic dehydrogenase (LDH) and lactic acid were monitored. Horses jumped obstacles of 1.10 to 1.20 m, and samples were taken before, immediately after and six hours following exercise. Cardiac rate was determined using a Polar S625 cardiac monitor; for respiratory rate, stethoscopes and blood samples were analyzed by spectophotometric methods. For muscle enzymes and lactic acid, statistic analysis (ANAVA) was carried out and for cardiac and respiratory rates descriptive statistics were performed. Results showed that lactic acid and creatinkinase had a notorious change with exercise, while lactic dehydrogenase had no alteration. Cardiac and respiratory rate had a significant increase after exercise, but return to normal in the next six hours. Lactic acid increased in a significant way and return to normal six hours after, unlike what other authors have reported. The research shows that changes are maybe due to type and time of exercise or altitude. Further research is necessary to complement topic related studies., El estudio presenta los primeros resultados de investigación en variables fisiológicas y sus cambios por el ejercicio en equinos en competencia de salto en el país. Se utilizaron 24 ejemplares de las razas Silla Argentina, PSI y mestizo de la Escuela de Equitación del Ejército Nacional de Colombia, que participaron en pruebas de salto (1,10 – 1,20 m) en diferentes centros ecuestres de la Sabana de Bogotá. Se determinó frecuencia cardiaca, frecuencia respiratoria y enzimas musculares (creatinquinasa (CK), lactato deshidrogenasa (LDH) y el ácido láctico).. Se tomaron tres muestras: reposo, inmediatamente después del ejercicio y a las 6 horas posejercicio. Para la frecuencia cardiaca se utilizó un monitor POLAR S625; la frecuencia respiratoria se determinó mediante fonendoscopio; y las enzimas y el ácido láctico se analizaron en laboratorio. Se utilizó el método estadístico ANAVA para enzimas musculares y acido láctico y estadística descriptiva para frecuencia cardiaca y respiratoria. En los resultados se encontró un comportamiento estadísticamente significativo (p < 0.05) del ácido láctico y la enzima creatinkinasa, a diferencia del comportamiento de la enzima lactato deshidrogenasa, que fue no significativo. Las frecuencias cardiaca y respiratoria se elevaron de manera significativa posejercicio y regresaron a lo normal alrededor de las 6 seis horas. El ácido láctico se incrementó posejercicio y disminuyó a las 6 horas, contrario a lo reportado por otros autores. Se concluye que una vez analizadas estas variables, hay diferencias con los valores obtenidos por otros autores, que pueden deberse a la altitud, al tipo y tiempo de ejercicio realizado. Por tanto es indispensable continuar haciendo estudios en este campo.

Preliminary investigation results in physiological traits and changes due to exercise (horse jumping competition) in equines in Colombia are presented. 24 horses were selected (Silla argentina, PSI and crossbred) at the National Army Cavalry school, where cardiac and respiratory rate, creatinkinase (CK), lactic dehydrogenase (LDH) and lactic acid were monitored. Horses jumped obstacles of 1.10 to 1.20 m, and samples were taken before, immediately after and six hours following exercise. Cardiac rate was determined using a Polar S625 cardiac monitor; for respiratory rate, stethoscopes and blood samples were analyzed by spectophotometric methods. For muscle enzymes and lactic acid, statistic analysis (ANAVA) was carried out and for cardiac and respiratory rates descriptive statistics were performed. Results showed that lactic acid and creatinkinase had a notorious change with exercise, while lactic dehydrogenase had no alteration. Cardiac and respiratory rate had a significant increase after exercise, but return to normal in the next six hours. Lactic acid increased in a significant way and return to normal six hours after, unlike what other authors have reported. The research shows that changes are maybe due to type and time of exercise or altitude. Further research is necessary to complement topic related studies., El estudio presenta los primeros resultados de investigación en variables fisiológicas y sus cambios por el ejercicio en equinos en competencia de salto en el país. Se utilizaron 24 ejemplares de las razas Silla Argentina, PSI y mestizo de la Escuela de Equitación del Ejército Nacional de Colombia, que participaron en pruebas de salto (1,10 – 1,20 m) en diferentes centros ecuestres de la Sabana de Bogotá. Se determinó frecuencia cardiaca, frecuencia respiratoria y enzimas musculares (creatinquinasa (CK), lactato deshidrogenasa (LDH) y el ácido láctico).. Se tomaron tres muestras: reposo, inmediatamente después del ejercicio y a las 6 horas posejercicio. Para la frecuencia cardiaca se utilizó un monitor POLAR S625; la frecuencia respiratoria se determinó mediante fonendoscopio; y las enzimas y el ácido láctico se analizaron en laboratorio. Se utilizó el método estadístico ANAVA para enzimas musculares y acido láctico y estadística descriptiva para frecuencia cardiaca y respiratoria. En los resultados se encontró un comportamiento estadísticamente significativo (p < 0.05) del ácido láctico y la enzima creatinkinasa, a diferencia del comportamiento de la enzima lactato deshidrogenasa, que fue no significativo. Las frecuencias cardiaca y respiratoria se elevaron de manera significativa posejercicio y regresaron a lo normal alrededor de las 6 seis horas. El ácido láctico se incrementó posejercicio y disminuyó a las 6 horas, contrario a lo reportado por otros autores. Se concluye que una vez analizadas estas variables, hay diferencias con los valores obtenidos por otros autores, que pueden deberse a la altitud, al tipo y tiempo de ejercicio realizado. Por tanto es indispensable continuar haciendo estudios en este campo.

Two previously healthy women developed an inflammatory myopathy before the term of their first pregnancy. Skeletal muscle biopsy led to a diagnosis of T cell-mediated polymyositis. Both babies were healthy, but their serum creatine kinase levels remained elevated for a few months after birth. Their mothers did well after corticosteroid treatment.

Observations made in oxygen K emission of CO2 show that the symmetry selective character of resonant x-ray emission is excitation energy dependent. Electronically dipole forbidden O K emission lines are observed for excitation at the O 1s --> pi* resonance, and they are gradually reduced upon detuning the excitation energy below threshold. The occurrence of forbidden transitions is explained in terms of dynamical symmetry breaking due to vibronic coupling, and the observation of quenching of the symmetry breaking upon detuning as due to an effective quenching of this vibronic coupling. Scattering theory applied to a few-level system is shown to account for the energy dependence of the symmetry selection in the limit of narrow bandpass photon excitation.

RIVM rapport:Van het RIVM humaan juistheidscontroleserum voor enzymactiviteitsmethingen is de stabiliteit na reconsitutie gedurende een week bij diverse bewaaromstandigheden onderzocht. Bij normale gebruiksomstandigheden werden na 24 uur geen afwijkingen geconstateerd. Door bacteriele besmetting veroorzaakte afwijkingen na langere tijd kunnen worden tegengegaan door toevoeging van natriumazide., not available

Van het RIVM humaan juistheidscontroleserum voor enzymactiviteitsmethingen is de stabiliteit na reconsitutie gedurende een week bij diverse bewaaromstandigheden onderzocht. Bij normale gebruiksomstandigheden werden na 24 uur geen afwijkingen geconstateerd. Door bacteriele besmetting veroorzaakte afwijkingen na langere tijd kunnen worden tegengegaan door toevoeging van natriumazide., not available

RIVM rapport:Van het RIVM humaan juistheidscontroleserum voor enzymactiviteitsmethingen is de stabiliteit na reconsitutie gedurende een week bij diverse bewaaromstandigheden onderzocht. Bij normale gebruiksomstandigheden werden na 24 uur geen afwijkingen geconstateerd. Door bacteriele besmetting veroorzaakte afwijkingen na langere tijd kunnen worden tegengegaan door toevoeging van natriumazide., not available

RIVM rapport:Van het RIVM humaan juistheidscontroleserum voor enzymactiviteitsmethingen is de stabiliteit na reconsitutie gedurende een week bij diverse bewaaromstandigheden onderzocht. Bij normale gebruiksomstandigheden werden na 24 uur geen afwijkingen geconstateerd. Door bacteriele besmetting veroorzaakte afwijkingen na langere tijd kunnen worden tegengegaan door toevoeging van natriumazide., not available

Pravidelná pohybová aktivita je považována za nezbytnou součást našeho života. Sport je doporučován pro předcházení různým chorobám, posílení imunitního systému či pro udržování váhy a zlepšení kondice člověka. Avšak v jaké míře je sportovní aktivita lidskému zdraví prospěšná a kdy přechází do stavu, kdy tělu spíše škodí? Tato práce pojednává o možných škodlivých účincích ultravytrvalostních sportů na lidský organismu. V našem případě se jedná o 100km běh, tedy ultramaraton a MTB. V následujících částech je snaha o vysvětlení metabolických změn a vlivu ultra vytrvalostního sportu na imunologické a biochemické parametry. Zkoumány byly parametry imunoglobulin A, M, leukocyty, kreatinin, sodné a draselné ionty a enzymy jako laktátdehydrogenáza, kreatinkináza a alaninaminotransferáza. Hodnoty těchto parametrů byly získány ze vzorků krve závodníků odebrané před závodem a následně hned po závodě. Bylo zkoumáno celkem 23 vzorků z cyklistického závodu na 24 hodin a 24 vzorků z běžeckého závodu na 100 km. Pro statistickou analýzu dat byl vytvořen nástroj v programu Matlab. Byl zjištěn enormní nárůst množství leukocytů v obou typech závodů. V cyklistickém závodě se jedná o navýšení z 5,75±1,29 x109/l na 11,80±3,08 x109/l, v běžeckém závodě z 4,99±1,84 x109/l na 13,20±5,36 x109/l. IgA se významně nesnížil, naopak se mírně zvýšil. Hodnoty IgM se u běžců výrazně nezměnily. U cyklistů došlo ke statisticky významnému snížení z původního množství 60,23±6,54 mg/dl na 52,60±10,43 mg/dl. U běžeckého závodu dále došlo ke statisticky významným změnám hladiny CK a LDH. Z původních hodnot CK 4,25±2,74 kat/l stoupla aktivita enzymu vlivem závodu na 50,09±60,08 kat/l. LDH se u cyklistického závodu nijak významně neliší, stejně jako je tomu u enzymu ALT. V tomto případě nedošlo k jeho statisticky významné změně ani v jednom typu závodu. Koncentrace draselných a sodných iontů významně poklesla. U draslíku jeho koncentrace poklesla z 5,61±0,59 mmol/l na 4,57±0,39 mmol/l. Koncentrace sodných io, Regular sport activity is considered as neccessary part of our lifes. Sport is reccomended for preventing various diseases, enhancing immunity system or for controlling weight and for enhancing conditions. However, how severaly is sport activity benefit for human health and when it becomes harmful for it? This work deals with possible negative effects of strenous excercise on human organism. In our case it is ultra-maraton and mountbiking. In following parts of this work it is tried to explaine metabolic changes and influance of strenous excercise on immunological and biochemical parametres. The parameters immunoglobulin A, M, leukocytes, creatinine, sodium and potassium ions and enzymes such as lactatedehydrogenase, creatinekinase and alanineaminotransferase were studied. The values of these parameters were obtained from the blood samples taken before and after the race. A 23 samples from a 24-hour cycling race and 24 run-of-the-run race samples per 100 km were examined. A matlab tool was created for statistical analysis of data. A huge increase in the number of leukocytes was found in both types of races. In the MTB, it is an increase from 5.75 ± 1.29 x 109 / l to 11.80 ± 3.08 x 109 / l, in the race from 4.99 ± 1.84 x 109 / l to 13, 20 ± 5.36 x 109 / l. IgA did not significantly decrease, on the contrary slightly increased. IgM values have not changed significantly in runners. Cyclists experienced a statistically significant reduction in the original amount of 60.23 ± 6.54 mg / dl to 52.60 ± 10.43 mg / dl. There were statistically significant changes in CK and LDH levels in the race. From the original CK values of 4.25 ± 2.74 kat / l, the activity of the enzyme increased by 50.09 ± 60.08 kat / l due to the race. LDH is not significantly different in the cycling race as well as in the ALT enzyme. In this case, there was no statistically significant change to either type of race. Concentration of potassium and sodium ions decreased significantly. In potassium, its c

Konvoluční neuronové sítě se dnes používají v mnoha oblastech, především ale pro strojové učení, kde vykazují velkou úspěšnost. Tato práce nejprve představí existující frameworky, další algoritmy pro rozpoznávání a pak popisuje, jak probíhalo vytváření vlastní datové sady a trénink modelu pro rozpoznávání emocí. Tento model má úspěšnost klasifikace 60%. Model je následně využit pro získání statistik o emocích z filmových trailerů a z těchto statistik je sestaven model pro rozpoznávání žánrů, který je konečně použit v naší aplikaci pro určení žánru vstupního traileru s přesností až 47%., Convolutional neural networks are used for various tasks, but foremost in machine learning, in which they excel. This work is going to introduce some existing frameworks, other algorithms for recognition and then we describe the training dataset creation and the model for emotion recognition training process. Mentioned model has accuracy of 60%. It is used for emotion statistics retrieval from movie trailers. Model for genre recognition is created from those statistics and then finally used in our application for genre recognition of the input trailer, with best accuracy of 47%.

Konvoluční neuronové sítě se dnes používají v mnoha oblastech, především ale pro strojové učení, kde vykazují velkou úspěšnost. Tato práce nejprve představí existující frameworky, další algoritmy pro rozpoznávání a pak popisuje, jak probíhalo vytváření vlastní datové sady a trénink modelu pro rozpoznávání emocí. Tento model má úspěšnost klasifikace 60%. Model je následně využit pro získání statistik o emocích z filmových trailerů a z těchto statistik je sestaven model pro rozpoznávání žánrů, který je konečně použit v naší aplikaci pro určení žánru vstupního traileru s přesností až 47%., Convolutional neural networks are used for various tasks, but foremost in machine learning, in which they excel. This work is going to introduce some existing frameworks, other algorithms for recognition and then we describe the training dataset creation and the model for emotion recognition training process. Mentioned model has accuracy of 60%. It is used for emotion statistics retrieval from movie trailers. Model for genre recognition is created from those statistics and then finally used in our application for genre recognition of the input trailer, with best accuracy of 47%.

Konvoluční neuronové sítě se dnes používají v mnoha oblastech, především ale pro strojové učení, kde vykazují velkou úspěšnost. Tato práce nejprve představí existující frameworky, další algoritmy pro rozpoznávání a pak popisuje, jak probíhalo vytváření vlastní datové sady a trénink modelu pro rozpoznávání emocí. Tento model má úspěšnost klasifikace 60%. Model je následně využit pro získání statistik o emocích z filmových trailerů a z těchto statistik je sestaven model pro rozpoznávání žánrů, který je konečně použit v naší aplikaci pro určení žánru vstupního traileru s přesností až 47%., Convolutional neural networks are used for various tasks, but foremost in machine learning, in which they excel. This work is going to introduce some existing frameworks, other algorithms for recognition and then we describe the training dataset creation and the model for emotion recognition training process. Mentioned model has accuracy of 60%. It is used for emotion statistics retrieval from movie trailers. Model for genre recognition is created from those statistics and then finally used in our application for genre recognition of the input trailer, with best accuracy of 47%.

Pravidelná pohybová aktivita je považována za nezbytnou součást našeho života. Sport je doporučován pro předcházení různým chorobám, posílení imunitního systému či pro udržování váhy a zlepšení kondice člověka. Avšak v jaké míře je sportovní aktivita lidskému zdraví prospěšná a kdy přechází do stavu, kdy tělu spíše škodí? Tato práce pojednává o možných škodlivých účincích ultravytrvalostních sportů na lidský organismu. V našem případě se jedná o 100km běh, tedy ultramaraton a MTB. V následujících částech je snaha o vysvětlení metabolických změn a vlivu ultra vytrvalostního sportu na imunologické a biochemické parametry. Zkoumány byly parametry imunoglobulin A, M, leukocyty, kreatinin, sodné a draselné ionty a enzymy jako laktátdehydrogenáza, kreatinkináza a alaninaminotransferáza. Hodnoty těchto parametrů byly získány ze vzorků krve závodníků odebrané před závodem a následně hned po závodě. Bylo zkoumáno celkem 23 vzorků z cyklistického závodu na 24 hodin a 24 vzorků z běžeckého závodu na 100 km. Pro statistickou analýzu dat byl vytvořen nástroj v programu Matlab. Byl zjištěn enormní nárůst množství leukocytů v obou typech závodů. V cyklistickém závodě se jedná o navýšení z 5,75±1,29 x109/l na 11,80±3,08 x109/l, v běžeckém závodě z 4,99±1,84 x109/l na 13,20±5,36 x109/l. IgA se významně nesnížil, naopak se mírně zvýšil. Hodnoty IgM se u běžců výrazně nezměnily. U cyklistů došlo ke statisticky významnému snížení z původního množství 60,23±6,54 mg/dl na 52,60±10,43 mg/dl. U běžeckého závodu dále došlo ke statisticky významným změnám hladiny CK a LDH. Z původních hodnot CK 4,25±2,74 kat/l stoupla aktivita enzymu vlivem závodu na 50,09±60,08 kat/l. LDH se u cyklistického závodu nijak významně neliší, stejně jako je tomu u enzymu ALT. V tomto případě nedošlo k jeho statisticky významné změně ani v jednom typu závodu. Koncentrace draselných a sodných iontů významně poklesla. U draslíku jeho koncentrace poklesla z 5,61±0,59 mmol/l na 4,57±0,39 mmol/l. Koncentrace sodných io, Regular sport activity is considered as neccessary part of our lifes. Sport is reccomended for preventing various diseases, enhancing immunity system or for controlling weight and for enhancing conditions. However, how severaly is sport activity benefit for human health and when it becomes harmful for it? This work deals with possible negative effects of strenous excercise on human organism. In our case it is ultra-maraton and mountbiking. In following parts of this work it is tried to explaine metabolic changes and influance of strenous excercise on immunological and biochemical parametres. The parameters immunoglobulin A, M, leukocytes, creatinine, sodium and potassium ions and enzymes such as lactatedehydrogenase, creatinekinase and alanineaminotransferase were studied. The values of these parameters were obtained from the blood samples taken before and after the race. A 23 samples from a 24-hour cycling race and 24 run-of-the-run race samples per 100 km were examined. A matlab tool was created for statistical analysis of data. A huge increase in the number of leukocytes was found in both types of races. In the MTB, it is an increase from 5.75 ± 1.29 x 109 / l to 11.80 ± 3.08 x 109 / l, in the race from 4.99 ± 1.84 x 109 / l to 13, 20 ± 5.36 x 109 / l. IgA did not significantly decrease, on the contrary slightly increased. IgM values have not changed significantly in runners. Cyclists experienced a statistically significant reduction in the original amount of 60.23 ± 6.54 mg / dl to 52.60 ± 10.43 mg / dl. There were statistically significant changes in CK and LDH levels in the race. From the original CK values of 4.25 ± 2.74 kat / l, the activity of the enzyme increased by 50.09 ± 60.08 kat / l due to the race. LDH is not significantly different in the cycling race as well as in the ALT enzyme. In this case, there was no statistically significant change to either type of race. Concentration of potassium and sodium ions decreased significantly. In potassium, its c

Pravidelná pohybová aktivita je považována za nezbytnou součást našeho života. Sport je doporučován pro předcházení různým chorobám, posílení imunitního systému či pro udržování váhy a zlepšení kondice člověka. Avšak v jaké míře je sportovní aktivita lidskému zdraví prospěšná a kdy přechází do stavu, kdy tělu spíše škodí? Tato práce pojednává o možných škodlivých účincích ultravytrvalostních sportů na lidský organismu. V našem případě se jedná o 100km běh, tedy ultramaraton a MTB. V následujících částech je snaha o vysvětlení metabolických změn a vlivu ultra vytrvalostního sportu na imunologické a biochemické parametry. Zkoumány byly parametry imunoglobulin A, M, leukocyty, kreatinin, sodné a draselné ionty a enzymy jako laktátdehydrogenáza, kreatinkináza a alaninaminotransferáza. Hodnoty těchto parametrů byly získány ze vzorků krve závodníků odebrané před závodem a následně hned po závodě. Bylo zkoumáno celkem 23 vzorků z cyklistického závodu na 24 hodin a 24 vzorků z běžeckého závodu na 100 km. Pro statistickou analýzu dat byl vytvořen nástroj v programu Matlab. Byl zjištěn enormní nárůst množství leukocytů v obou typech závodů. V cyklistickém závodě se jedná o navýšení z 5,75±1,29 x109/l na 11,80±3,08 x109/l, v běžeckém závodě z 4,99±1,84 x109/l na 13,20±5,36 x109/l. IgA se významně nesnížil, naopak se mírně zvýšil. Hodnoty IgM se u běžců výrazně nezměnily. U cyklistů došlo ke statisticky významnému snížení z původního množství 60,23±6,54 mg/dl na 52,60±10,43 mg/dl. U běžeckého závodu dále došlo ke statisticky významným změnám hladiny CK a LDH. Z původních hodnot CK 4,25±2,74 kat/l stoupla aktivita enzymu vlivem závodu na 50,09±60,08 kat/l. LDH se u cyklistického závodu nijak významně neliší, stejně jako je tomu u enzymu ALT. V tomto případě nedošlo k jeho statisticky významné změně ani v jednom typu závodu. Koncentrace draselných a sodných iontů významně poklesla. U draslíku jeho koncentrace poklesla z 5,61±0,59 mmol/l na 4,57±0,39 mmol/l. Koncentrace sodných io, Regular sport activity is considered as neccessary part of our lifes. Sport is reccomended for preventing various diseases, enhancing immunity system or for controlling weight and for enhancing conditions. However, how severaly is sport activity benefit for human health and when it becomes harmful for it? This work deals with possible negative effects of strenous excercise on human organism. In our case it is ultra-maraton and mountbiking. In following parts of this work it is tried to explaine metabolic changes and influance of strenous excercise on immunological and biochemical parametres. The parameters immunoglobulin A, M, leukocytes, creatinine, sodium and potassium ions and enzymes such as lactatedehydrogenase, creatinekinase and alanineaminotransferase were studied. The values of these parameters were obtained from the blood samples taken before and after the race. A 23 samples from a 24-hour cycling race and 24 run-of-the-run race samples per 100 km were examined. A matlab tool was created for statistical analysis of data. A huge increase in the number of leukocytes was found in both types of races. In the MTB, it is an increase from 5.75 ± 1.29 x 109 / l to 11.80 ± 3.08 x 109 / l, in the race from 4.99 ± 1.84 x 109 / l to 13, 20 ± 5.36 x 109 / l. IgA did not significantly decrease, on the contrary slightly increased. IgM values have not changed significantly in runners. Cyclists experienced a statistically significant reduction in the original amount of 60.23 ± 6.54 mg / dl to 52.60 ± 10.43 mg / dl. There were statistically significant changes in CK and LDH levels in the race. From the original CK values of 4.25 ± 2.74 kat / l, the activity of the enzyme increased by 50.09 ± 60.08 kat / l due to the race. LDH is not significantly different in the cycling race as well as in the ALT enzyme. In this case, there was no statistically significant change to either type of race. Concentration of potassium and sodium ions decreased significantly. In potassium, its c

Konvoluční neuronové sítě se dnes používají v mnoha oblastech, především ale pro strojové učení, kde vykazují velkou úspěšnost. Tato práce nejprve představí existující frameworky, další algoritmy pro rozpoznávání a pak popisuje, jak probíhalo vytváření vlastní datové sady a trénink modelu pro rozpoznávání emocí. Tento model má úspěšnost klasifikace 60%. Model je následně využit pro získání statistik o emocích z filmových trailerů a z těchto statistik je sestaven model pro rozpoznávání žánrů, který je konečně použit v naší aplikaci pro určení žánru vstupního traileru s přesností až 47%., Convolutional neural networks are used for various tasks, but foremost in machine learning, in which they excel. This work is going to introduce some existing frameworks, other algorithms for recognition and then we describe the training dataset creation and the model for emotion recognition training process. Mentioned model has accuracy of 60%. It is used for emotion statistics retrieval from movie trailers. Model for genre recognition is created from those statistics and then finally used in our application for genre recognition of the input trailer, with best accuracy of 47%.

Konvoluční neuronové sítě se dnes používají v mnoha oblastech, především ale pro strojové učení, kde vykazují velkou úspěšnost. Tato práce nejprve představí existující frameworky, další algoritmy pro rozpoznávání a pak popisuje, jak probíhalo vytváření vlastní datové sady a trénink modelu pro rozpoznávání emocí. Tento model má úspěšnost klasifikace 60%. Model je následně využit pro získání statistik o emocích z filmových trailerů a z těchto statistik je sestaven model pro rozpoznávání žánrů, který je konečně použit v naší aplikaci pro určení žánru vstupního traileru s přesností až 47%., Convolutional neural networks are used for various tasks, but foremost in machine learning, in which they excel. This work is going to introduce some existing frameworks, other algorithms for recognition and then we describe the training dataset creation and the model for emotion recognition training process. Mentioned model has accuracy of 60%. It is used for emotion statistics retrieval from movie trailers. Model for genre recognition is created from those statistics and then finally used in our application for genre recognition of the input trailer, with best accuracy of 47%.

Konvoluční neuronové sítě se dnes používají v mnoha oblastech, především ale pro strojové učení, kde vykazují velkou úspěšnost. Tato práce nejprve představí existující frameworky, další algoritmy pro rozpoznávání a pak popisuje, jak probíhalo vytváření vlastní datové sady a trénink modelu pro rozpoznávání emocí. Tento model má úspěšnost klasifikace 60%. Model je následně využit pro získání statistik o emocích z filmových trailerů a z těchto statistik je sestaven model pro rozpoznávání žánrů, který je konečně použit v naší aplikaci pro určení žánru vstupního traileru s přesností až 47%., Convolutional neural networks are used for various tasks, but foremost in machine learning, in which they excel. This work is going to introduce some existing frameworks, other algorithms for recognition and then we describe the training dataset creation and the model for emotion recognition training process. Mentioned model has accuracy of 60%. It is used for emotion statistics retrieval from movie trailers. Model for genre recognition is created from those statistics and then finally used in our application for genre recognition of the input trailer, with best accuracy of 47%.

Pravidelná pohybová aktivita je považována za nezbytnou součást našeho života. Sport je doporučován pro předcházení různým chorobám, posílení imunitního systému či pro udržování váhy a zlepšení kondice člověka. Avšak v jaké míře je sportovní aktivita lidskému zdraví prospěšná a kdy přechází do stavu, kdy tělu spíše škodí? Tato práce pojednává o možných škodlivých účincích ultravytrvalostních sportů na lidský organismu. V našem případě se jedná o 100km běh, tedy ultramaraton a MTB. V následujících částech je snaha o vysvětlení metabolických změn a vlivu ultra vytrvalostního sportu na imunologické a biochemické parametry. Zkoumány byly parametry imunoglobulin A, M, leukocyty, kreatinin, sodné a draselné ionty a enzymy jako laktátdehydrogenáza, kreatinkináza a alaninaminotransferáza. Hodnoty těchto parametrů byly získány ze vzorků krve závodníků odebrané před závodem a následně hned po závodě. Bylo zkoumáno celkem 23 vzorků z cyklistického závodu na 24 hodin a 24 vzorků z běžeckého závodu na 100 km. Pro statistickou analýzu dat byl vytvořen nástroj v programu Matlab. Byl zjištěn enormní nárůst množství leukocytů v obou typech závodů. V cyklistickém závodě se jedná o navýšení z 5,75±1,29 x109/l na 11,80±3,08 x109/l, v běžeckém závodě z 4,99±1,84 x109/l na 13,20±5,36 x109/l. IgA se významně nesnížil, naopak se mírně zvýšil. Hodnoty IgM se u běžců výrazně nezměnily. U cyklistů došlo ke statisticky významnému snížení z původního množství 60,23±6,54 mg/dl na 52,60±10,43 mg/dl. U běžeckého závodu dále došlo ke statisticky významným změnám hladiny CK a LDH. Z původních hodnot CK 4,25±2,74 kat/l stoupla aktivita enzymu vlivem závodu na 50,09±60,08 kat/l. LDH se u cyklistického závodu nijak významně neliší, stejně jako je tomu u enzymu ALT. V tomto případě nedošlo k jeho statisticky významné změně ani v jednom typu závodu. Koncentrace draselných a sodných iontů významně poklesla. U draslíku jeho koncentrace poklesla z 5,61±0,59 mmol/l na 4,57±0,39 mmol/l. Koncentrace sodných io, Regular sport activity is considered as neccessary part of our lifes. Sport is reccomended for preventing various diseases, enhancing immunity system or for controlling weight and for enhancing conditions. However, how severaly is sport activity benefit for human health and when it becomes harmful for it? This work deals with possible negative effects of strenous excercise on human organism. In our case it is ultra-maraton and mountbiking. In following parts of this work it is tried to explaine metabolic changes and influance of strenous excercise on immunological and biochemical parametres. The parameters immunoglobulin A, M, leukocytes, creatinine, sodium and potassium ions and enzymes such as lactatedehydrogenase, creatinekinase and alanineaminotransferase were studied. The values of these parameters were obtained from the blood samples taken before and after the race. A 23 samples from a 24-hour cycling race and 24 run-of-the-run race samples per 100 km were examined. A matlab tool was created for statistical analysis of data. A huge increase in the number of leukocytes was found in both types of races. In the MTB, it is an increase from 5.75 ± 1.29 x 109 / l to 11.80 ± 3.08 x 109 / l, in the race from 4.99 ± 1.84 x 109 / l to 13, 20 ± 5.36 x 109 / l. IgA did not significantly decrease, on the contrary slightly increased. IgM values have not changed significantly in runners. Cyclists experienced a statistically significant reduction in the original amount of 60.23 ± 6.54 mg / dl to 52.60 ± 10.43 mg / dl. There were statistically significant changes in CK and LDH levels in the race. From the original CK values of 4.25 ± 2.74 kat / l, the activity of the enzyme increased by 50.09 ± 60.08 kat / l due to the race. LDH is not significantly different in the cycling race as well as in the ALT enzyme. In this case, there was no statistically significant change to either type of race. Concentration of potassium and sodium ions decreased significantly. In potassium, its c

Konvoluční neuronové sítě se dnes používají v mnoha oblastech, především ale pro strojové učení, kde vykazují velkou úspěšnost. Tato práce nejprve představí existující frameworky, další algoritmy pro rozpoznávání a pak popisuje, jak probíhalo vytváření vlastní datové sady a trénink modelu pro rozpoznávání emocí. Tento model má úspěšnost klasifikace 60%. Model je následně využit pro získání statistik o emocích z filmových trailerů a z těchto statistik je sestaven model pro rozpoznávání žánrů, který je konečně použit v naší aplikaci pro určení žánru vstupního traileru s přesností až 47%., Convolutional neural networks are used for various tasks, but foremost in machine learning, in which they excel. This work is going to introduce some existing frameworks, other algorithms for recognition and then we describe the training dataset creation and the model for emotion recognition training process. Mentioned model has accuracy of 60%. It is used for emotion statistics retrieval from movie trailers. Model for genre recognition is created from those statistics and then finally used in our application for genre recognition of the input trailer, with best accuracy of 47%.

Pravidelná pohybová aktivita je považována za nezbytnou součást našeho života. Sport je doporučován pro předcházení různým chorobám, posílení imunitního systému či pro udržování váhy a zlepšení kondice člověka. Avšak v jaké míře je sportovní aktivita lidskému zdraví prospěšná a kdy přechází do stavu, kdy tělu spíše škodí? Tato práce pojednává o možných škodlivých účincích ultravytrvalostních sportů na lidský organismu. V našem případě se jedná o 100km běh, tedy ultramaraton a MTB. V následujících částech je snaha o vysvětlení metabolických změn a vlivu ultra vytrvalostního sportu na imunologické a biochemické parametry. Zkoumány byly parametry imunoglobulin A, M, leukocyty, kreatinin, sodné a draselné ionty a enzymy jako laktátdehydrogenáza, kreatinkináza a alaninaminotransferáza. Hodnoty těchto parametrů byly získány ze vzorků krve závodníků odebrané před závodem a následně hned po závodě. Bylo zkoumáno celkem 23 vzorků z cyklistického závodu na 24 hodin a 24 vzorků z běžeckého závodu na 100 km. Pro statistickou analýzu dat byl vytvořen nástroj v programu Matlab. Byl zjištěn enormní nárůst množství leukocytů v obou typech závodů. V cyklistickém závodě se jedná o navýšení z 5,75±1,29 x109/l na 11,80±3,08 x109/l, v běžeckém závodě z 4,99±1,84 x109/l na 13,20±5,36 x109/l. IgA se významně nesnížil, naopak se mírně zvýšil. Hodnoty IgM se u běžců výrazně nezměnily. U cyklistů došlo ke statisticky významnému snížení z původního množství 60,23±6,54 mg/dl na 52,60±10,43 mg/dl. U běžeckého závodu dále došlo ke statisticky významným změnám hladiny CK a LDH. Z původních hodnot CK 4,25±2,74 kat/l stoupla aktivita enzymu vlivem závodu na 50,09±60,08 kat/l. LDH se u cyklistického závodu nijak významně neliší, stejně jako je tomu u enzymu ALT. V tomto případě nedošlo k jeho statisticky významné změně ani v jednom typu závodu. Koncentrace draselných a sodných iontů významně poklesla. U draslíku jeho koncentrace poklesla z 5,61±0,59 mmol/l na 4,57±0,39 mmol/l. Koncentrace sodných io, Regular sport activity is considered as neccessary part of our lifes. Sport is reccomended for preventing various diseases, enhancing immunity system or for controlling weight and for enhancing conditions. However, how severaly is sport activity benefit for human health and when it becomes harmful for it? This work deals with possible negative effects of strenous excercise on human organism. In our case it is ultra-maraton and mountbiking. In following parts of this work it is tried to explaine metabolic changes and influance of strenous excercise on immunological and biochemical parametres. The parameters immunoglobulin A, M, leukocytes, creatinine, sodium and potassium ions and enzymes such as lactatedehydrogenase, creatinekinase and alanineaminotransferase were studied. The values of these parameters were obtained from the blood samples taken before and after the race. A 23 samples from a 24-hour cycling race and 24 run-of-the-run race samples per 100 km were examined. A matlab tool was created for statistical analysis of data. A huge increase in the number of leukocytes was found in both types of races. In the MTB, it is an increase from 5.75 ± 1.29 x 109 / l to 11.80 ± 3.08 x 109 / l, in the race from 4.99 ± 1.84 x 109 / l to 13, 20 ± 5.36 x 109 / l. IgA did not significantly decrease, on the contrary slightly increased. IgM values have not changed significantly in runners. Cyclists experienced a statistically significant reduction in the original amount of 60.23 ± 6.54 mg / dl to 52.60 ± 10.43 mg / dl. There were statistically significant changes in CK and LDH levels in the race. From the original CK values of 4.25 ± 2.74 kat / l, the activity of the enzyme increased by 50.09 ± 60.08 kat / l due to the race. LDH is not significantly different in the cycling race as well as in the ALT enzyme. In this case, there was no statistically significant change to either type of race. Concentration of potassium and sodium ions decreased significantly. In potassium, its c

Konvoluční neuronové sítě se dnes používají v mnoha oblastech, především ale pro strojové učení, kde vykazují velkou úspěšnost. Tato práce nejprve představí existující frameworky, další algoritmy pro rozpoznávání a pak popisuje, jak probíhalo vytváření vlastní datové sady a trénink modelu pro rozpoznávání emocí. Tento model má úspěšnost klasifikace 60%. Model je následně využit pro získání statistik o emocích z filmových trailerů a z těchto statistik je sestaven model pro rozpoznávání žánrů, který je konečně použit v naší aplikaci pro určení žánru vstupního traileru s přesností až 47%., Convolutional neural networks are used for various tasks, but foremost in machine learning, in which they excel. This work is going to introduce some existing frameworks, other algorithms for recognition and then we describe the training dataset creation and the model for emotion recognition training process. Mentioned model has accuracy of 60%. It is used for emotion statistics retrieval from movie trailers. Model for genre recognition is created from those statistics and then finally used in our application for genre recognition of the input trailer, with best accuracy of 47%.