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The genome sequence of the moderately halophilic Lamprobacter modestohalophilus ShNLb02 was compared to those of other Lamprobacter and Halochromatium species. It revealed an average nucleotide identity of 94% to Lpb. modestohalophilus DSM 25653 and of 89.7% to Halochromatium roseum DSM 18859, underscoring their close phylogenetic relationship.

Several strains of a Gram-negative, anaerobic photoautotrophic, motile, rod-shaped bacterium, designated as B14B, A-7R, and A-7Y were isolated from biofilms of low-mineralized soda lakes in central Mongolia and Russia (southeast Siberia). They had lamellar stacks as photosynthetic structures and bacteriochlorophyll a as the major photosynthetic pigment. The strains were found to grow at 25–35 °C, pH 7.5–10.2 (optimum, pH 9.0), and with 0–8% (w/v) NaCl (optimum, 0%). In the presence of sulfide and bicarbonate, acetate, butyrate, yeast extract, lactate, malate, pyruvate, succinate, and fumarate promoted growth. The DNA G + C content was 62.9–63.0 mol%. While the 16S rRNA gene sequences confirmed that the new strains belonged to the genus Ectothiorhodospira of the Ectothiorhodospiraceae, comparison of the genome nucleotide sequences of strains B14B, A-7R, and A-7Y revealed that the new isolates were remote from all described Ectothiorhodospira species both in dDDH (19.7–38.8%) and in ANI (75.0–89.4%). The new strains are also genetically differentiated by the presence of a nitric oxide reduction pathway that is lacking from all other Ectiothiorhodospiraceae. We propose to assign the isolates to the new species, Ectothiorhodospira lacustris sp. nov., with the type strain B14BT (=DSM 116064T = KCTC 25542T = UQM 41491T).

Changes in the morphology and structure of the GaAs surface during the deposition of an Au film by thermal evaporation in vacuum have been studied. It has been found that the deposition of an Au film with the participation of a flow of particles and light from a heated evaporator causes the appearance of photo effects in the near-surface GaAs layers, including light diffraction on surface acoustic waves, the growth of whiskers, and electron emission, which leads to the formation of microcracks on the GaAs surface and the growth of GaAs crystallites. It is shown that the structure and composition of the film boundaries of Au and GaAs surfaces depend on the electron concentration in gallium arsenide, which ultimately determines the properties of the electrophysical parameters of the Au-GaAs contacts., QC 20230810

Several strains of a Gram-negative, anaerobic photoautotrophic, motile, rod-shaped bacterium, designated as B14B, A-7R, and A-7Y were isolated from biofilms of low-mineralized soda lakes in central Mongolia and Russia (southeast Siberia). They had lamellar stacks as photosynthetic structures and bacteriochlorophyll a as the major photosynthetic pigment. The strains were found to grow at 25–35 °C, pH 7.5–10.2 (optimum, pH 9.0), and with 0–8% (w/v) NaCl (optimum, 0%). In the presence of sulfide and bicarbonate, acetate, butyrate, yeast extract, lactate, malate, pyruvate, succinate, and fumarate promoted growth. The DNA G + C content was 62.9–63.0 mol%. While the 16S rRNA gene sequences confirmed that the new strains belonged to the genus Ectothiorhodospira of the Ectothiorhodospiraceae, comparison of the genome nucleotide sequences of strains B14B, A-7R, and A-7Y revealed that the new isolates were remote from all described Ectothiorhodospira species both in dDDH (19.7–38.8%) and in ANI (75.0–89.4%). The new strains are also genetically differentiated by the presence of a nitric oxide reduction pathway that is lacking from all other Ectiothiorhodospiraceae. We propose to assign the isolates to the new species, Ectothiorhodospira lacustris sp. nov., with the type strain B14BT (=DSM 116064T = KCTC 25542T = UQM 41491T).

Several strains of a Gram-negative, anaerobic photoautotrophic, motile, rod-shaped bacterium, designated as B14B, A-7R, and A-7Y were isolated from biofilms of low-mineralized soda lakes in central Mongolia and Russia (southeast Siberia). They had lamellar stacks as photosynthetic structures and bacteriochlorophyll a as the major photosynthetic pigment. The strains were found to grow at 25–35 °C, pH 7.5–10.2 (optimum, pH 9.0), and with 0–8% (w/v) NaCl (optimum, 0%). In the presence of sulfide and bicarbonate, acetate, butyrate, yeast extract, lactate, malate, pyruvate, succinate, and fumarate promoted growth. The DNA G + C content was 62.9–63.0 mol%. While the 16S rRNA gene sequences confirmed that the new strains belonged to the genus Ectothiorhodospira of the Ectothiorhodospiraceae, comparison of the genome nucleotide sequences of strains B14B, A-7R, and A-7Y revealed that the new isolates were remote from all described Ectothiorhodospira species both in dDDH (19.7–38.8%) and in ANI (75.0–89.4%). The new strains are also genetically differentiated by the presence of a nitric oxide reduction pathway that is lacking from all other Ectiothiorhodospiraceae. We propose to assign the isolates to the new species, Ectothiorhodospira lacustris sp. nov., with the type strain B14BT (=DSM 116064T = KCTC 25542T = UQM 41491T).

The literature review highlights the current epidemiological situation of HIV and tuberculosis co-infection in the world and in Uzbekistan. The literature data on the development of various forms of tuberculosis in HIV-infected patients, clinical manifestations of tuberculosis, peculiarities of the course, diagnosis and prevention are presented.

The literature review highlights the current epidemiological situation of HIV and tuberculosis co-infection in the world and in Uzbekistan. The literature data on the development of various forms of tuberculosis in HIV-infected patients, clinical manifestations of tuberculosis, peculiarities of the course, diagnosis and prevention are presented.

The literature review highlights the current epidemiological situation of HIV and tuberculosis co-infection in the world and in Uzbekistan. The literature data on the development of various forms of tuberculosis in HIV-infected patients, clinical manifestations of tuberculosis, peculiarities of the course, diagnosis and prevention are presented.

The literature review highlights the current epidemiological situation of HIV and tuberculosis co-infection in the world and in Uzbekistan. The literature data on the development of various forms of tuberculosis in HIV-infected patients, clinical manifestations of tuberculosis, peculiarities of the course, diagnosis and prevention are presented.

The literature review highlights the current epidemiological situation of HIV and tuberculosis co-infection in the world and in Uzbekistan. The literature data on the development of various forms of tuberculosis in HIV-infected patients, clinical manifestations of tuberculosis, peculiarities of the course, diagnosis and prevention are presented.

The literature review highlights the current epidemiological situation of HIV and tuberculosis co-infection in the world and in Uzbekistan. The literature data on the development of various forms of tuberculosis in HIV-infected patients, clinical manifestations of tuberculosis, peculiarities of the course, diagnosis and prevention are presented.

The literature review highlights the current epidemiological situation of HIV and tuberculosis co-infection in the world and in Uzbekistan. The literature data on the development of various forms of tuberculosis in HIV-infected patients, clinical manifestations of tuberculosis, peculiarities of the course, diagnosis and prevention are presented.

The literature review highlights the current epidemiological situation of HIV and tuberculosis co-infection in the world and in Uzbekistan. The literature data on the development of various forms of tuberculosis in HIV-infected patients, clinical manifestations of tuberculosis, peculiarities of the course, diagnosis and prevention are presented.

The ferromagnetic resonance (FMR) in the frequency range of 0.5 to 12.5 GHz has been investigated as a function of external magnetic field for rapidly quenched Fe3Co67Cr3Si15B12 amorphous ribbons with different features of the effective magnetic anisotropy. Three states of the ribbons were considered: as-quenched without any treatment; after relaxation annealing without stress at the temperature of 350 °C during 1 h; and after annealing under specific stress of 230 MPa at the temperature of 350 °C during 1 h. For FMR measurements, we adapted a technique previously proposed and tested for the case of microwires. Here, amorphous ribbons were studied using the sample holder based on a commercial SMA connector. On the basis of the measurements of the reflection coefficient S11, the total impedance including its real and imaginary components was determined to be in the frequency range of 0.5 to 12.5 GHz. In order to confirm the validity of the proposed technique, FMR was also measured by the certified cavity perturbation technique using a commercial Bruker spectrometer operating at X-band frequency of 9.39 GHz. As part of the characterization of the ribbons used for microwave measurements, comparative analysis was performed of X-ray diffraction, optical microscopy, transmission electron microscopy, inductive magnetic hysteresis loops, vibrating sample magnetometry, magneto-optical Kerr effect (including magnetic domains) and magnetoimpedance data for of all samples.

The ferromagnetic resonance (FMR) in the frequency range of 0.5 to 12.5 GHz has been investigated as a function of external magnetic field for rapidly quenched Fe3Co67Cr3Si15B12 amorphous ribbons with different features of the effective magnetic anisotropy. Three states of the ribbons were considered: as-quenched without any treatment; after relaxation annealing without stress at the temperature of 350 °C during 1 h; and after annealing under specific stress of 230 MPa at the temperature of 350 °C during 1 h. For FMR measurements, we adapted a technique previously proposed and tested for the case of microwires. Here, amorphous ribbons were studied using the sample holder based on a commercial SMA connector. On the basis of the measurements of the reflection coefficient S11, the total impedance including its real and imaginary components was determined to be in the frequency range of 0.5 to 12.5 GHz. In order to confirm the validity of the proposed technique, FMR was also measured by the certified cavity perturbation technique using a commercial Bruker spectrometer operating at X-band frequency of 9.39 GHz. As part of the characterization of the ribbons used for microwave measurements, comparative analysis was performed of X-ray diffraction, optical microscopy, transmission electron microscopy, inductive magnetic hysteresis loops, vibrating sample magnetometry, magneto-optical Kerr effect (including magnetic domains) and magnetoimpedance data for of all samples.

Meningococcal disease (MD) is a serious public health care problem in most countries of the world, which is related to a high mortality and disability rate of people who have had this disease. The decline in the incidence of MD in the interepidemic period, which lasts more than 30 years in Uzbekistan, has led to a decrease the alertness of medical workers regarding this infection. Late diagnosis and hospitalization are often the reasons for untimely medical care.The analyse of the the age and sex structure of morbidity in meningococcal infection, serogroup landscape of the pathogen, clinical forms of meningococcal infection at the present stage was given in this article.

Before being introduced into clinical practice in the 1960s. measles vaccine, measles has been one of the leading causes of childhood morbidity and mortality worldwide, with more than 2 million deaths annually. In 2012, a new Global Strategic Plan for Measles Control began.However, it has not been possible to achieve the elimination of measles or maintain the achieved status at the regional level, and in recent years there has been an alarming increase in the number of cases of measles and deaths from it around the world, while sometimes cases of importation of infection in the presence of even small groups of unvaccinated people lead to the occurrence of large outbreaks of the disease.The article describes some of the epidemiological features of measles infection in Uzbekistan at the present stage.

WHO has announced the Covid-19 pandemic, which has spread throughout the world. Vaccination is the only way to fight this infection. On April 1, the process of vaccination of the population against coronavirus began in Uzbekistan.Three vaccines are used in the Republic of Uzbekistan (ZF-UZ-VAC 2001, Astra Zeneca and SPUTNIK '' V ''). The article describes the vaccination coverage of citizens of Uzbekistan at stage 1 for the period from April 1 to May 7

WHO has announced the Covid-19 pandemic, which has spread throughout the world. Vaccination is the only way to fight this infection. On April 1, the process of vaccination of the population against coronavirus began in Uzbekistan.Three vaccines are used in the Republic of Uzbekistan (ZF-UZ-VAC 2001, Astra Zeneca and SPUTNIK '' V ''). The article describes the vaccination coverage of citizens of Uzbekistan at stage 1 for the period from April 1 to May 7

Meningococcal disease (MD) is a serious public health care problem in most countries of the world, which is related to a high mortality and disability rate of people who have had this disease. The decline in the incidence of MD in the interepidemic period, which lasts more than 30 years in Uzbekistan, has led to a decrease the alertness of medical workers regarding this infection. Late diagnosis and hospitalization are often the reasons for untimely medical care.The analyse of the the age and sex structure of morbidity in meningococcal infection, serogroup landscape of the pathogen, clinical forms of meningococcal infection at the present stage was given in this article.

Before being introduced into clinical practice in the 1960s. measles vaccine, measles has been one of the leading causes of childhood morbidity and mortality worldwide, with more than 2 million deaths annually. In 2012, a new Global Strategic Plan for Measles Control began.However, it has not been possible to achieve the elimination of measles or maintain the achieved status at the regional level, and in recent years there has been an alarming increase in the number of cases of measles and deaths from it around the world, while sometimes cases of importation of infection in the presence of even small groups of unvaccinated people lead to the occurrence of large outbreaks of the disease.The article describes some of the epidemiological features of measles infection in Uzbekistan at the present stage.

WHO has announced the Covid-19 pandemic, which has spread throughout the world. Vaccination is the only way to fight this infection. On April 1, the process of vaccination of the population against coronavirus began in Uzbekistan.Three vaccines are used in the Republic of Uzbekistan (ZF-UZ-VAC 2001, Astra Zeneca and SPUTNIK '' V ''). The article describes the vaccination coverage of citizens of Uzbekistan at stage 1 for the period from April 1 to May 7

Meningococcal disease (MD) is a serious public health care problem in most countries of the world, which is related to a high mortality and disability rate of people who have had this disease. The decline in the incidence of MD in the interepidemic period, which lasts more than 30 years in Uzbekistan, has led to a decrease the alertness of medical workers regarding this infection. Late diagnosis and hospitalization are often the reasons for untimely medical care.The analyse of the the age and sex structure of morbidity in meningococcal infection, serogroup landscape of the pathogen, clinical forms of meningococcal infection at the present stage was given in this article.

Before being introduced into clinical practice in the 1960s. measles vaccine, measles has been one of the leading causes of childhood morbidity and mortality worldwide, with more than 2 million deaths annually. In 2012, a new Global Strategic Plan for Measles Control began.However, it has not been possible to achieve the elimination of measles or maintain the achieved status at the regional level, and in recent years there has been an alarming increase in the number of cases of measles and deaths from it around the world, while sometimes cases of importation of infection in the presence of even small groups of unvaccinated people lead to the occurrence of large outbreaks of the disease.The article describes some of the epidemiological features of measles infection in Uzbekistan at the present stage.

Before being introduced into clinical practice in the 1960s. measles vaccine, measles has been one of the leading causes of childhood morbidity and mortality worldwide, with more than 2 million deaths annually. In 2012, a new Global Strategic Plan for Measles Control began.However, it has not been possible to achieve the elimination of measles or maintain the achieved status at the regional level, and in recent years there has been an alarming increase in the number of cases of measles and deaths from it around the world, while sometimes cases of importation of infection in the presence of even small groups of unvaccinated people lead to the occurrence of large outbreaks of the disease.The article describes some of the epidemiological features of measles infection in Uzbekistan at the present stage.

WHO has announced the Covid-19 pandemic, which has spread throughout the world. Vaccination is the only way to fight this infection. On April 1, the process of vaccination of the population against coronavirus began in Uzbekistan.Three vaccines are used in the Republic of Uzbekistan (ZF-UZ-VAC 2001, Astra Zeneca and SPUTNIK '' V ''). The article describes the vaccination coverage of citizens of Uzbekistan at stage 1 for the period from April 1 to May 7

Meningococcal disease (MD) is a serious public health care problem in most countries of the world, which is related to a high mortality and disability rate of people who have had this disease. The decline in the incidence of MD in the interepidemic period, which lasts more than 30 years in Uzbekistan, has led to a decrease the alertness of medical workers regarding this infection. Late diagnosis and hospitalization are often the reasons for untimely medical care.The analyse of the the age and sex structure of morbidity in meningococcal infection, serogroup landscape of the pathogen, clinical forms of meningococcal infection at the present stage was given in this article.

WHO has announced the Covid-19 pandemic, which has spread throughout the world. Vaccination is the only way to fight this infection. On April 1, the process of vaccination of the population against coronavirus began in Uzbekistan.Three vaccines are used in the Republic of Uzbekistan (ZF-UZ-VAC 2001, Astra Zeneca and SPUTNIK '' V ''). The article describes the vaccination coverage of citizens of Uzbekistan at stage 1 for the period from April 1 to May 7

Meningococcal disease (MD) is a serious public health care problem in most countries of the world, which is related to a high mortality and disability rate of people who have had this disease. The decline in the incidence of MD in the interepidemic period, which lasts more than 30 years in Uzbekistan, has led to a decrease the alertness of medical workers regarding this infection. Late diagnosis and hospitalization are often the reasons for untimely medical care.The analyse of the the age and sex structure of morbidity in meningococcal infection, serogroup landscape of the pathogen, clinical forms of meningococcal infection at the present stage was given in this article.

Before being introduced into clinical practice in the 1960s. measles vaccine, measles has been one of the leading causes of childhood morbidity and mortality worldwide, with more than 2 million deaths annually. In 2012, a new Global Strategic Plan for Measles Control began.However, it has not been possible to achieve the elimination of measles or maintain the achieved status at the regional level, and in recent years there has been an alarming increase in the number of cases of measles and deaths from it around the world, while sometimes cases of importation of infection in the presence of even small groups of unvaccinated people lead to the occurrence of large outbreaks of the disease.The article describes some of the epidemiological features of measles infection in Uzbekistan at the present stage.

Meningococcal disease (MD) is a serious public health care problem in most countries of the world, which is related to a high mortality and disability rate of people who have had this disease. The decline in the incidence of MD in the interepidemic period, which lasts more than 30 years in Uzbekistan, has led to a decrease the alertness of medical workers regarding this infection. Late diagnosis and hospitalization are often the reasons for untimely medical care.The analyse of the the age and sex structure of morbidity in meningococcal infection, serogroup landscape of the pathogen, clinical forms of meningococcal infection at the present stage was given in this article.

WHO has announced the Covid-19 pandemic, which has spread throughout the world. Vaccination is the only way to fight this infection. On April 1, the process of vaccination of the population against coronavirus began in Uzbekistan.Three vaccines are used in the Republic of Uzbekistan (ZF-UZ-VAC 2001, Astra Zeneca and SPUTNIK '' V ''). The article describes the vaccination coverage of citizens of Uzbekistan at stage 1 for the period from April 1 to May 7

Before being introduced into clinical practice in the 1960s. measles vaccine, measles has been one of the leading causes of childhood morbidity and mortality worldwide, with more than 2 million deaths annually. In 2012, a new Global Strategic Plan for Measles Control began.However, it has not been possible to achieve the elimination of measles or maintain the achieved status at the regional level, and in recent years there has been an alarming increase in the number of cases of measles and deaths from it around the world, while sometimes cases of importation of infection in the presence of even small groups of unvaccinated people lead to the occurrence of large outbreaks of the disease.The article describes some of the epidemiological features of measles infection in Uzbekistan at the present stage.

Before being introduced into clinical practice in the 1960s. measles vaccine, measles has been one of the leading causes of childhood morbidity and mortality worldwide, with more than 2 million deaths annually. In 2012, a new Global Strategic Plan for Measles Control began.However, it has not been possible to achieve the elimination of measles or maintain the achieved status at the regional level, and in recent years there has been an alarming increase in the number of cases of measles and deaths from it around the world, while sometimes cases of importation of infection in the presence of even small groups of unvaccinated people lead to the occurrence of large outbreaks of the disease.The article describes some of the epidemiological features of measles infection in Uzbekistan at the present stage.

Meningococcal disease (MD) is a serious public health care problem in most countries of the world, which is related to a high mortality and disability rate of people who have had this disease. The decline in the incidence of MD in the interepidemic period, which lasts more than 30 years in Uzbekistan, has led to a decrease the alertness of medical workers regarding this infection. Late diagnosis and hospitalization are often the reasons for untimely medical care.The analyse of the the age and sex structure of morbidity in meningococcal infection, serogroup landscape of the pathogen, clinical forms of meningococcal infection at the present stage was given in this article.

WHO has announced the Covid-19 pandemic, which has spread throughout the world. Vaccination is the only way to fight this infection. On April 1, the process of vaccination of the population against coronavirus began in Uzbekistan.Three vaccines are used in the Republic of Uzbekistan (ZF-UZ-VAC 2001, Astra Zeneca and SPUTNIK '' V ''). The article describes the vaccination coverage of citizens of Uzbekistan at stage 1 for the period from April 1 to May 7

Проведены исследования сорбции никеля из сернокислых растворов выщелачивания окисленных никелевых руд. Показано, что иониты с бис-пиколиламиновыми и иминодиацетатными группами проявляют способность к сорбции ионов никеля (II) даже на фоне преобладающих концентраций ионов железа (III). Наиболее эффективным оказался ионит Lewatit TP220. Сорбция никеля с удовлетворительными показателями протекает как в варианте сорбционного выщелачивания, так и извлечения из осветленных растворов. При сорбции из пульпы емкость по никелю составила 5,44 мг/г, по железу 25,17 мг/г., Studies of nickel sorption from sulfuric acid leachates of oxidized nickel ores have been carried out. It was shown that ion exchangers with bis-picolylamine and iminodiacetate groups exhibit the ability to adsorb nickel (II) ions even against the background of prevailing concentrations of iron (III) ions. The most effective was the ion exchanger Lewatit TP220 and, to a lesser extent, Lewatit TP207. Sorption of nickel with satisfactory performance occurs both in the variant of resin-in-pulp and from clarified solutions. During sorption from the pulp, the capacity for nickel was 5.44 mg/g, for iron, 25.17 mg/g.

The problem of processing galvanic sludges, formed as a result of neutralization of technological solutions and wastewater containing heavy non-ferrous metals is considered. At present, sludges are transported to disposal area and are not used in any way. Typically, such sludges contain significant amounts of chromium and nickel, which creates environmental hazard. Investigated sludge contains up to 6.6% Ni and up to 7.4% Cr. The hydrometallurgical technology for processing of these sludges has been developed. Solutions of sulfuric acid and ammonia were used as lixiviants. It is shown that when using a solution of sulfuric acid with pH=1.5, extraction of up to 93.3% Cu, 70.2 Ni, 90.3 Zn is achieved. For selective recovery of nickel from leachates the process of sorption concentration by Lewatit TP207 is proposed. © Published under licence by IOP Publishing Ltd.

We investigate functional role of the P76GTKMIFA83 fragment of the primary structure of cytochrome c. Based on the data obtained by the analysis of informational structure (ANIS), we propose a model of functioning of cytochrome c. According to this model, conformational rearrangements of the P76GTKMIFA83 loop fragment have a significant effect on conformational mobility of the heme. It is suggested that the conformational mobility of cytochrome c heme is responsible for its optimal orientation with respect to electron donor and acceptor within ubiquinol-cytochrome c oxidoreductase (complex III) and cytochrome c oxidase (complex IV), respectively, thus, ensuring electron transfer from complex III to complex IV. To validate the model, we design several mutant variants of horse cytochrome c with multiple substitutions of amino acid residues in the P76GTKMIFA83 sequence that reduce its ability to undergo conformational rearrangements. With this, we study the succinate-cytochrome c reductase and cytochrome c oxidase activities of rat liver mitoplasts in the presence of mutant variants of cytochrome c. The electron transport activity of the mutant variants decreases to different extent. Resonance Raman spectroscopy (RRS) and surface-enhanced Raman spectroscopy (SERS) data demonstrate, that all mutant cytochromes possess heme with the higher degree of ruffling deformation, than that of the wild-type (WT) cytochrome c. The increase in the ruffled deformation of the heme of oxidized cytochromes correlated with the decrease in the electron transport rate of ubiquinol-cytochrome c reductase (complex III). Besides, all mutant cytochromes have lower mobility of the pyrrol rings and methine bridges, than WT cytochrome c. We show that a decrease in electron transport activity in the mutant variants correlates with conformational changes and reduced mobility of heme porphyrin. This points to a significant role of the P76GTKMIFA83 fragment in the electron transport function of cy

We investigate functional role of the P76GTKMIFA83 fragment of the primary structure of cytochrome c. Based on the data obtained by the analysis of informational structure (ANIS), we propose a model of functioning of cytochrome c. According to this model, conformational rearrangements of the P76GTKMIFA83 loop fragment have a significant effect on conformational mobility of the heme. It is suggested that the conformational mobility of cytochrome c heme is responsible for its optimal orientation with respect to electron donor and acceptor within ubiquinol-cytochrome c oxidoreductase (complex III) and cytochrome c oxidase (complex IV), respectively, thus, ensuring electron transfer from complex III to complex IV. To validate the model, we design several mutant variants of horse cytochrome c with multiple substitutions of amino acid residues in the P76GTKMIFA83 sequence that reduce its ability to undergo conformational rearrangements. With this, we study the succinate-cytochrome c reductase and cytochrome c oxidase activities of rat liver mitoplasts in the presence of mutant variants of cytochrome c. The electron transport activity of the mutant variants decreases to different extent. Resonance Raman spectroscopy (RRS) and surface-enhanced Raman spectroscopy (SERS) data demonstrate, that all mutant cytochromes possess heme with the higher degree of ruffling deformation, than that of the wild-type (WT) cytochrome c. The increase in the ruffled deformation of the heme of oxidized cytochromes correlated with the decrease in the electron transport rate of ubiquinol-cytochrome c reductase (complex III). Besides, all mutant cytochromes have lower mobility of the pyrrol rings and methine bridges, than WT cytochrome c. We show that a decrease in electron transport activity in the mutant variants correlates with conformational changes and reduced mobility of heme porphyrin. This points to a significant role of the P76GTKMIFA83 fragment in the electron transport function of cy

Tatyana Y Kuznetsova,1 Viktoria A Korneva,1 Evgeniya N Bryantseva,2 Vitaliy S Barkan,2 Artemy V Orlov,3 Igor N Posokhov,4 Anatoly N Rogoza51Faculty of Medicine, Petrozavodsk State University, Petrozavodsk, Russia; 2Diagnostics Department, The hospital within the Russian Railroad Network, Chita, Russia; 3Department 65 Competitive System Analysis, National Research Nuclear University MEPhI, Moscow, Russia; 4Hemodynamic Laboratory Ltd, Nizhniy Novgorod, Russia; 5Cardiology Research Center, Moscow, RussiaAbstract: The purpose of this study was to examine the pulse wave velocity, aortic augmentation index corrected for heart rate 75 (AIx@75), and central systolic and diastolic blood pressure during 24-hour monitoring in normotensive volunteers. Overall, 467 subjects (206 men and 261 women) were recruited in this study. Participants were excluded from the study if they were less than 19 years of age, had blood test abnormalities, had a body mass index greater than 27.5 kg/m2, had impaired glucose tolerance, or had hypotension or hypertension. Ambulatory blood pressure monitoring (ABPM) with the BPLab® device was performed in each subject. ABPM waveforms were analyzed using the special automatic Vasotens® algorithm, which allows the calculation of pulse wave velocity, AIx@75, central systolic and diastolic blood pressure for “24-hour”, “awake”, and “asleep” periods. Circadian rhythms and sex differences in these indexes were identified. Pending further validation in prospective outcome-based studies, our data may be used as preliminary diagnostic values for the BPLab ABPM additional index in adult subjects.Keywords: ambulatory, monitoring, arterial stiffness, augmentation index, central blood pressure, reference values

Cryolithological, ground ice and fossil bioindicator (pollen, diatoms, plant macrofossils, rhizopods, insects, mammal bones) records from Bol'shoy Lyakhovsky Island permafrost sequences (73°20′N, 141°30′E) document the environmental history in the region for the past c. 115 kyr. Vegetation similar to modern subarctic tundra communities prevailed during the Eemian/Early Weichselian transition with a climate warmer than the present. Sparse tundra-like vegetation and harsher climate conditions were predominant during the Early Weichselian. The Middle Weichselian deposits contain peat and peaty soil horizons with bioindicators documenting climate amelioration. Although dwarf willows grew in more protected places, tundra and steppe vegetation prevailed. Climate conditions became colder and drier c. 30 kyr BP. No sediments dated between c. 28.5 and 12.05 14C kyr BP were found, which may reflect active erosion during that time. Herb and shrubby vegetation were predominant 11.6–11.3 14C kyr BP. Summer temperatures were c. 4 °C higher than today. Typical arctic environments prevailed around 10.5 14C kyr BP. Shrub alder and dwarf birch tundra were predominant between c. 9 and 7.6 kyr BP. Reconstructed summer temperatures were at least 4 °C higher than present. However, insect remains reflect that steppe-like habitats existed until c. 8 kyr BP. After 7.6 kyr BP, shrubs gradually disappeared and the vegetation cover became similar to that of modern tundra. Pollen and beetles indicate a severe arctic environment c. 3.7 kyr BP. However, Betula nana, absent on the island today, was still present. Together with our previous study on Bol'shoy Lyakhovsky Island covering the period between about 200 and 115 kyr, a comprehensive terrestrial palaeoenvironmental data set from this area in western Beringia is now available for the past two glacial–interglacial cycles.

During studies of moderately halophilic strains of Ectothiorhodospira from steppe soda lakes, we found a novel group of bacteria related to Ectothiorhodospira haloalkaliphila with salt optima at 50–80 g NaCl l”1. Phylogenetic analysis using 16S rRNA gene sequences of strains from soda lakes in Mongolia, Egypt and Siberia revealed separation of the group of new isolates from other Ectothiorhodospira species, including the closely related Ect. haloalkaliphila. DNA–DNA hybridization studies demonstrated that the new isolates form a homogeneous group at the species level, but at the same time are distinct from related species such as Ect. haloalkaliphila, Ect. vacuolata, Ect. shaposhnikovii and Ect. marina. The new isolates are considered to be strains of a novel species, for which the name Ectothiorhodospira variabilis sp. nov. is proposed, with the type strain WN22T (5VKM B-2479T 5DSM 21381T). Photosynthetic pigments of the novel species are bacteriochlorophyll a and carotenoids of the spirilloxanthin series with spirilloxanthin and derivatives thereof, together with small amounts of lycopene and rhodopin. Gas vesicles are formed by most of the strains, particularly in media containing yeast extract (0.5 g l”1) and acetate (0.5–2.0 g l”1). Sequence analysis of nifH (nitrogenase) and cbbL (RuBisCO) confirmed the assignment of the strains to the genus Ectothiorhodospira and in particular the close relationship to Ect. haloalkaliphila. The novel species Ect. variabilis is found in soda lakes separated by great geographical distances and is an alkaliphilic and halophilic bacterium that tolerates salt concentrations up to 150–200 g NaCl l”1.

During studies of moderately halophilic strains of Ectothiorhodospira from steppe soda lakes, we found a novel group of bacteria related to Ectothiorhodospira haloalkaliphila with salt optima at 50–80 g NaCl l”1. Phylogenetic analysis using 16S rRNA gene sequences of strains from soda lakes in Mongolia, Egypt and Siberia revealed separation of the group of new isolates from other Ectothiorhodospira species, including the closely related Ect. haloalkaliphila. DNA–DNA hybridization studies demonstrated that the new isolates form a homogeneous group at the species level, but at the same time are distinct from related species such as Ect. haloalkaliphila, Ect. vacuolata, Ect. shaposhnikovii and Ect. marina. The new isolates are considered to be strains of a novel species, for which the name Ectothiorhodospira variabilis sp. nov. is proposed, with the type strain WN22T (5VKM B-2479T 5DSM 21381T). Photosynthetic pigments of the novel species are bacteriochlorophyll a and carotenoids of the spirilloxanthin series with spirilloxanthin and derivatives thereof, together with small amounts of lycopene and rhodopin. Gas vesicles are formed by most of the strains, particularly in media containing yeast extract (0.5 g l”1) and acetate (0.5–2.0 g l”1). Sequence analysis of nifH (nitrogenase) and cbbL (RuBisCO) confirmed the assignment of the strains to the genus Ectothiorhodospira and in particular the close relationship to Ect. haloalkaliphila. The novel species Ect. variabilis is found in soda lakes separated by great geographical distances and is an alkaliphilic and halophilic bacterium that tolerates salt concentrations up to 150–200 g NaCl l”1.

Cryolithological, ground ice and fossil bioindicator (pollen, diatoms, plant macrofossils, rhizopods, insects, mammal bones) records from Bol'shoy Lyakhovsky Island permafrost sequences (73°20′N, 141°30′E) document the environmental history in the region for the past c. 115 kyr. Vegetation similar to modern subarctic tundra communities prevailed during the Eemian/Early Weichselian transition with a climate warmer than the present. Sparse tundra-like vegetation and harsher climate conditions were predominant during the Early Weichselian. The Middle Weichselian deposits contain peat and peaty soil horizons with bioindicators documenting climate amelioration. Although dwarf willows grew in more protected places, tundra and steppe vegetation prevailed. Climate conditions became colder and drier c. 30 kyr BP. No sediments dated between c. 28.5 and 12.05 14C kyr BP were found, which may reflect active erosion during that time. Herb and shrubby vegetation were predominant 11.6–11.3 14C kyr BP. Summer temperatures were c. 4 °C higher than today. Typical arctic environments prevailed around 10.5 14C kyr BP. Shrub alder and dwarf birch tundra were predominant between c. 9 and 7.6 kyr BP. Reconstructed summer temperatures were at least 4 °C higher than present. However, insect remains reflect that steppe-like habitats existed until c. 8 kyr BP. After 7.6 kyr BP, shrubs gradually disappeared and the vegetation cover became similar to that of modern tundra. Pollen and beetles indicate a severe arctic environment c. 3.7 kyr BP. However, Betula nana, absent on the island today, was still present. Together with our previous study on Bol'shoy Lyakhovsky Island covering the period between about 200 and 115 kyr, a comprehensive terrestrial palaeoenvironmental data set from this area in western Beringia is now available for the past two glacial–interglacial cycles.

During studies of moderately halophilic strains of Ectothiorhodospira from steppe soda lakes, we found a novel group of bacteria related to Ectothiorhodospira haloalkaliphila with salt optima at 50–80 g NaCl l”1. Phylogenetic analysis using 16S rRNA gene sequences of strains from soda lakes in Mongolia, Egypt and Siberia revealed separation of the group of new isolates from other Ectothiorhodospira species, including the closely related Ect. haloalkaliphila. DNA–DNA hybridization studies demonstrated that the new isolates form a homogeneous group at the species level, but at the same time are distinct from related species such as Ect. haloalkaliphila, Ect. vacuolata, Ect. shaposhnikovii and Ect. marina. The new isolates are considered to be strains of a novel species, for which the name Ectothiorhodospira variabilis sp. nov. is proposed, with the type strain WN22T (5VKM B-2479T 5DSM 21381T). Photosynthetic pigments of the novel species are bacteriochlorophyll a and carotenoids of the spirilloxanthin series with spirilloxanthin and derivatives thereof, together with small amounts of lycopene and rhodopin. Gas vesicles are formed by most of the strains, particularly in media containing yeast extract (0.5 g l”1) and acetate (0.5–2.0 g l”1). Sequence analysis of nifH (nitrogenase) and cbbL (RuBisCO) confirmed the assignment of the strains to the genus Ectothiorhodospira and in particular the close relationship to Ect. haloalkaliphila. The novel species Ect. variabilis is found in soda lakes separated by great geographical distances and is an alkaliphilic and halophilic bacterium that tolerates salt concentrations up to 150–200 g NaCl l”1.

Four strains of purple sulfur bacteria containing bacteriochlorophyll b were isolated from cyanobacterial mats of soda lakes in the steppe of south-east Siberia, Russia. Cells of all strains were cocci without gas vesicles. Eventually, cells with flagella were seen in the electron microscope, but motile cells were observed very rarely in cultures. Internal photosynthetic membranes were of the tubular type. Photosynthetic pigments were bacteriochlorophyll b and carotenoids with spectral characteristics similar to 3,4,3',4'-tetrahydrospirilloxanthin. The bacteria were obligately phototrophic and strictly anaerobic. Hydrogen sulfide and elemental sulfur were used as photosynthetic electron donors. Thiosulfate was not used. During growth on sulfide, sulfur globules were formed as intermediate oxidation products, deposited inside the cells and centrally located. In the presence of sulfide and sodium bicarbonate, acetate, malate, propionate, pyruvate, succinate, fumarate and yeast extract were photoassimilated. Growth factors were not required. The new bacterium is an obligate alkaliphile growing at pH 8-10 with an optimum at pH 9. It showed good growth up to 6.0% sodium chloride and up to 8.5% sodium carbonates. Phenotypically, it is similar to Thiococcus pfennigii, but different by virtue of its alkaliphily and salt tolerance. The DNA G+C content was 63.6-64.8 mol %, compared to 69.4-69.9 mol % for Thiococcus pfennigii. The 16S rDNA sequence of strain A26T was approximately 92% similar to that of Thiococcus pfennigii DSM 226 and therefore a new genus and species name, Thioalkalicoccus limnaeus gen. nov. and sp. nov., are proposed for the new bacterium

Four strains of purple sulfur bacteria containing bacteriochlorophyll b were isolated from cyanobacterial mats of soda lakes in the steppe of south-east Siberia, Russia. Cells of all strains were cocci without gas vesicles. Eventually, cells with flagella were seen in the electron microscope, but motile cells were observed very rarely in cultures. Internal photosynthetic membranes were of the tubular type. Photosynthetic pigments were bacteriochlorophyll b and carotenoids with spectral characteristics similar to 3,4,3',4'-tetrahydrospirilloxanthin. The bacteria were obligately phototrophic and strictly anaerobic. Hydrogen sulfide and elemental sulfur were used as photosynthetic electron donors. Thiosulfate was not used. During growth on sulfide, sulfur globules were formed as intermediate oxidation products, deposited inside the cells and centrally located. In the presence of sulfide and sodium bicarbonate, acetate, malate, propionate, pyruvate, succinate, fumarate and yeast extract were photoassimilated. Growth factors were not required. The new bacterium is an obligate alkaliphile growing at pH 8-10 with an optimum at pH 9. It showed good growth up to 6.0% sodium chloride and up to 8.5% sodium carbonates. Phenotypically, it is similar to Thiococcus pfennigii, but different by virtue of its alkaliphily and salt tolerance. The DNA G+C content was 63.6-64.8 mol %, compared to 69.4-69.9 mol % for Thiococcus pfennigii. The 16S rDNA sequence of strain A26T was approximately 92% similar to that of Thiococcus pfennigii DSM 226 and therefore a new genus and species name, Thioalkalicoccus limnaeus gen. nov. and sp. nov., are proposed for the new bacterium