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Wall wetting phenomena were investigated in a light-duty diesel optical engine operating under typical diesel particulate filter regeneration conditions through the use of liquid spray imaging during late-cycle post-injections. Three post-injection timings were explored: 1) an ‘early’ timing (44.5°aTDC) where high ambient temperatures and densities were expected to decrease the liquid penetration, 2) a ‘conventional’ timing (78.5°aTDC) that is typically used to produce the necessary aftertreatment regeneration exhaust conditions, and 3) a ‘late’ timing (133.5°aTDC) where in-cylinder flows generated by exhaust valve opening-induced blowdown can disrupt the liquid penetration. In addition to a 2007 US certification diesel fuel, a palm-derived B20 biodiesel blend and soy-derived B100 biodiesel were examined since liquid spray impingement is thought to worsen for biodiesel blends due to the higher fuel distillation temperature, density , and viscosity. No significant wall wetting was observed for the 44.5°aTDC post-injection SOI. However, considerable impingement occurred for the 78.5°aTDC and 133.5°aTDC SOI. Liquid penetration and persistence were found to increase with biodiesel content, while exhaust blowdown flows were ineffective in reducing the severity of wall wetting. Negligible distortion of jet structure was observed for the liquid spray at the late post-injection SOI. Implementation of multiple Sshort- pulse injectionsduration decreased liquid penetration at the early post-injection SOI and with the neat SME biodieselB100 fuel, showing potential to mitigate significant wall wetting only at this timing.