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3 results on '"Borghi MO"'

1. Synergistic activity of everolimus and 5-aza-2 '-deoxycytidine in medullary thyroid carcinoma cell lines

Author

Vitale, G (Giovanni), Dicitore, A, Pepe, D, Gentilini, D, Grassi, E S, Borghi, MO, Gelmini, G, Cantone, MC, Gaudenzi, G, Misso, G, Di Blasio, AM, Hofland, Leo, Caraglia, M, Persani, L, Vitale, G (Giovanni), Dicitore, A, Pepe, D, Gentilini, D, Grassi, E S, Borghi, MO, Gelmini, G, Cantone, MC, Gaudenzi, G, Misso, G, Di Blasio, AM, Hofland, Leo, Caraglia, M, and Persani, L

Published
2017

2. Decreased expression of heparin-binding epidermal growth factor-like growth factor as a newly identified pathogenic mechanism of antiphospholipid-mediated defective placentation

Author

Di Simone, Nicoletta, Marana, Riccardo, Castellani, Roberta, Di Nicuolo, Fiorella, D'Alessio, Mc, Raschi, E, Borghi, Mo, Chen, Pp, Sanguinetti, Maurizio, Caruso, Alessandro, Meroni, Pl, Di Simone, Nicoletta (ORCID:0000-0003-1273-3335), Marana, Riccardo (ORCID:0000-0003-1616-7836), Sanguinetti, Maurizio (ORCID:0000-0002-9780-7059), Caruso, Alessandro (ORCID:0000-0002-4749-3207), Di Simone, Nicoletta, Marana, Riccardo, Castellani, Roberta, Di Nicuolo, Fiorella, D'Alessio, Mc, Raschi, E, Borghi, Mo, Chen, Pp, Sanguinetti, Maurizio, Caruso, Alessandro, Meroni, Pl, Di Simone, Nicoletta (ORCID:0000-0003-1273-3335), Marana, Riccardo (ORCID:0000-0003-1616-7836), Sanguinetti, Maurizio (ORCID:0000-0002-9780-7059), and Caruso, Alessandro (ORCID:0000-0002-4749-3207)

Abstract

Heparin-binding epidermal growth factor-like growth factor (HB-EGF) plays a role in blastocyst implantation and is down-regulated in preeclampsia and in hypertensive pregnancy disorders associated with defective extravillous trophoblast invasion. Defective placentation and severe preeclampsia are also features of the antiphospholipid syndrome (APS). The purpose of this study was to investigate whether abnormal HB-EGF expression plays a pathogenic role in antiphospholipid antibody (aPL)-mediated defective placentation.

Published
2010

3. Anti-beta 2 glycoprotein I antibodies affect Bcl-2 and Bax trophoblast expression but without evidence of apoptosis

Author

Di Simone, Nicoletta, Castellani, Roberta, Raschi, E, Borghi, Mo, Meroni, Pier Luigi, Caruso, Alessandro, Di Simone, Nicoletta (ORCID:0000-0003-1273-3335), Caruso, Alessandro (ORCID:0000-0002-4749-3207), Di Simone, Nicoletta, Castellani, Roberta, Raschi, E, Borghi, Mo, Meroni, Pier Luigi, Caruso, Alessandro, Di Simone, Nicoletta (ORCID:0000-0003-1273-3335), and Caruso, Alessandro (ORCID:0000-0002-4749-3207)

Abstract

Antiphospholipid antibodies (aPLs) reacting with beta-2 glycoprotein I (beta2GPI) have been associated with recurrent fetal loss and pregnancy complications. The aim of the study was to investigate whether aPLs with anti-beta2GPI specificity induce apoptosis of human trophoblasts in vitro. To this end, human anti-beta2GPI monoclonal IgM derived from a patient with antiphospholipid syndrome and a human irrelevant monoclonal IgM were incubated with human trophoblast cell cultures for 24, 48, and 72 h. In all the cultures we evaluated: (i) Bcl-2 and Bax mRNA and protein expression by Western blot and reverse transcription polymerase chain reaction (RT-PCR), respectively; (ii) DNA fragmentation by a commercial ELISA kit and by agarose gel electrophoresis; and (iii) the percentage of cells reactive with the monoclonal antibody (MAb) M30 by indirect immunofluorescence. The results were: Bcl-2/Bax ratio increased in untreated trophoblast cells during the time of culture, showing the highest values detectable after 72 h (2.68 and 2.28 at protein and mRNA levels, respectively). Cell incubation with anti-beta2GPI MAbs induced a significant Bcl-2/Bax ratio reduction in comparison with untreated cells (1.22 and 1.28 at protein and mRNA levels, respectively, after 72 h incubation). No significant difference was detected after cell exposure to irrelevant MAbs. However, neither DNA fragmentation nor increase in cells positive for the caspase-cleaved epitope of cytokeratin 18 cytoskeletal protein (M30) was found. In Conclusion, anti-beta2GPI antibodies react with trophoblast cells and reduce the Bcl-2/Bax ratio, but without any clear apoptotic effect

Published
2006

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