1. Comprehensive analysis of the candidate genes CCL2, CCR2, and TLR4 in age-related macular degeneration
- Author
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Despriet, D.D.G. (Dominique), Bergen, A.A.B. (Arthur), Merriam, J.E. (Joanna), Zernant, J. (Jana), Barile, G.R. (Gaetano), Smith, T. (Tim), Barbazetto, I.A. (Irene), Soest, S. (Simone) van, Bakker, A. (Arne), Jong, P.T.V.M. (Paulus) de, Allikmets, R. (Rando), Klaver, C.C.W. (Caroline), Despriet, D.D.G. (Dominique), Bergen, A.A.B. (Arthur), Merriam, J.E. (Joanna), Zernant, J. (Jana), Barile, G.R. (Gaetano), Smith, T. (Tim), Barbazetto, I.A. (Irene), Soest, S. (Simone) van, Bakker, A. (Arne), Jong, P.T.V.M. (Paulus) de, Allikmets, R. (Rando), and Klaver, C.C.W. (Caroline)
- Abstract
PURPOSE. To determine whether variants in the candidate genes TLR4, CCL2, and CCR2 are associated with age-related macular degeneration (AMD). METHODS. This study was performed in two independent Caucasian populations that included 357 cases and 173 controls from the Netherlands and 368 cases and 368 controls from the United States. Exon 4 of the TLR4 gene and the promoter, all exons, and flanking intronic regions of the CCL2 and CCR2 genes were analyzed in the Dutch study and common variants were validated in the U.S. study. Quantitative (q)PCR reactions were performed to evaluate expression of these genes in laser-dissected retinal pigment epithelium from 13 donor AMD and 13 control eyes. RESULTS. Analysis of single nucleotide polymorphisms (SNPs) in the TLR4 gene did not show a significant association between D299G or T399I and AMD, nor did haplotypes containing these variants. Univariate analyses of the SNPs in CCL2 and CCR2 did not demonstrate an association with AMD. For CCR2, haplotype frequencies were not significantly different between cases and controls. For CCL2, one haplotype containing the minor allele of C35C was significantly associated with AMD
- Published
- 2008
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