Search

Poly(DL-lactide-co-glycolide) (PDLLGA) and poly(L-lactide-co-glycolide) (PLLGA) copolymers were prepared by bulk ring opening polymerization of lactide and glycolide and characterized by GPC, FTIR, 1H NMR and DSC. Copolymers with different molar masses at a constant lactide/glycolide ratio were used for preparation of bovine serum albumin (BSA)-loaded microparticles by the double emulsion w/o/w method. The influence of the copolymer molar mass and composition on the microparticle morphology, size, yield, degradation rate, BSA-loading efficiency and BSA release profile were studied. For microparticles prepared from PDLLGA copolymers, a biphasic profile for BSA release was found and for those made from PLLGA copolymers the release profile was typically triphasic; both of them were characterized by high initial burst release. Possible reasons for such behavior are discussed., Poli(DL-laktid-ko-glikolid) (PDLLGA) i poli(L-laktid-ko-glikolid) (PLLGA) kopolimeri priređeni su polimerizacijom laktida i glikolida uz otvaranje prstenova i karakterizirani pomoću GPC, FTIR, 1H NMR i DSC. Kopolimeri različitih molarnih masa i stalnog omjera laktida i glikolida upotrebljeni su za pripravu mikročestica s goveđim serumskim albuminom (BSA) metodom dvostruke emulzije tipa voda/ulje/voda. Proučavan je utjecaj molarne mase i sastava kopolimera na oblik, veličinu, iskorištenje i stupanj razgradnje mikročestica, uklapanje i oslobađanje BSA. Za mikročestice pripravljene s PDLLGA kopolimerom utvrđen je bifazični profil oslobađanja BSA, a za mikročestice s PLLGA kopolimerom trifazičan profil. Za obje vrste karakteristično je brzo početno oslobađanje. Razmatrani su mogući uzroci takvog ponašanja.