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Research on open-domain social dialogue systems has exploded over the last few years with the advent of large language models (LLMs) that can chat about any topic. Unlike traditional dialogue systems, open-domain dialogue systems cannot assume any specific information need or domain restrictions - the only inherent goal is to converse socially. While modern systems have access to more information and better tools, foundational components of natural human-human conversation remain elusive, i.e., intimacy and agency. In this thesis, we hypothesize that personalization is pivotal in fostering this genuine connection between users and open-domain dialogue systems.Our first hypothesis is that personalizing the conversation to specific user interests will build a sense of understanding, rapport, and agency. To investigate this, we heuristically combine the results of an extensive natural language understanding pipeline with handcrafted rules to build a user modeling mechanism; this user model then personalizes the experience through response adaptation and topic-promotion strategies, resulting in a statistically significant positive impact on perceived conversation quality and length when evaluated at scale with a testbed open-domain dialogue system, that real Amazon Echo users access. Analyzing the user models unveils nuanced insights into user preferences, emphasizing a desire for more personalized experiences and receptiveness toward personal questions. This leads to our second hypothesis - asking appropriate personalized follow-up questions (PQs) helps to create a more engaged user experience that increases user satisfaction. Our initial test of this hypothesis uses a crowdsourced corpus of PQs (Would You Rather and Hypothetical) in the testbed system's dialogue policy. Our evaluation of the policy shows that it results in extended topical depth, leading to statistically significant longer, more highly rated conversations.However, crowdsourcing PQs for every user inte

Objective Zero-alcohol beverages (<0.5% alcohol by volume) appear and taste similar to alcoholic beverages but are regulated similarly to soft drinks in many countries, blurring the distinction between alcoholic and nonalcoholic beverages. How parents view provision of zero-alcohol beverages to adolescents is likely a key determinant of adolescent consumption. We investigated factors associated with parents' provision of zeroalcohol beverages to adolescents, including attitudes toward zero-alcohol beverages and demographic, knowledge, and behavioural factors known to be associated with provision of alcoholic beverages. Methods We conducted an online cross-sectional survey of N = 1197 Australian parents of adolescents aged 12-17 years in April-May 2022. We examined associations with zero-alcohol beverage provision using binomial logistic regression, and with future provision intentions using multinomial logistic regression analyses. Results Factors significantly associated (p < .001) with parents' provision and future intentions to provide zeroalcohol beverages to their adolescent included beliefs that zero-alcohol beverages had benefits for adolescents (Adjusted Odds Ratio [AOR] 2.69 (provision); 3.72 (intentions)), provision of alcoholic beverages (AOR 2.67 (provision); 3.72 (intentions)), and an incorrect understanding of alcohol guidelines for adolescents (AOR 2.38 (provision); 1.95 (intentions)). Conclusions Parents' provision and intentions to provide zero-alcohol beverages were associated with beliefs about zero-alcohol beverages as well as some factors associated with provision of alcoholic beverages. Precautionary advice to parents that the provision of zero-alcohol beverages may serve to normalise alcohol consumption may be warranted.

OBJECTIVE: To replicate a seven-factor model previously reported for the Delis-Kaplan Executive Function System (D-KEFS). METHOD: This study used the D-KEFS standardization sample including 1,750 non-clinical participants. Several seven-factor models previously reported for the D-KEFS were re-evaluated using confirmatory factor analysis (CFA). Previously published bi-factor models were also tested. These models were compared with a three-factor a priori model based on Cattell-Horn-Carroll (CHC) theory. Measurement invariance was examined across three age cohorts. RESULTS: All previously reported models failed to converge when tested with CFA. None of the bi-factor models converged after large numbers of iterations, suggesting that bi-factor models are ill-suited to represent the D-KEFS scores as reported in the test manual. Although poor fit was initially observed for the three-factor CHC model, inspection of modification indices showed potential for improvement by including method effects via correlated residuals for scores derived from similar tests. The final CHC model showed good to excellent fit and strong metric measurement invariance across the three age cohorts with minor exceptions for a subset of Fluency parameters. CONCLUSIONS: CHC theory extends to the D-KEFS, supporting findings from previous studies that executive functions can be integrated into CHC theory.

Objective: Clinical Practice Guidelines (CPGs) aim to support management of hip and knee osteoarthritis (OA), but recommendations are often conflicting and implementation is poor, contributing to evidence-to-practice gaps. This systematic review investigated the contextual and methodological factors contributing to conflicting recommendations for hip and knee OA. Method: Our systematic review appraised CPGs for managing hip and knee OA in adults ≥18 years (PROSPERO CRD42021276635). We used AGREE-II and AGREE-REX to assess quality and extracted data on treatment gaps, conflicts, biases, and consensus. Heterogeneity of recommendations was determined using Weighted Fleiss Kappa (K). The relationship between (K) and AGREE-II/AGREE-REX scores was explored. Results: We identified 25 CPGs across eight countries and four international organisations. The ACR, EULAR, NICE, OARSI and RACGP guidelines scored highest for overall AGREE-II quality (83%). The highest overall AGREE-REX scores were for BMJ Arthroscopy (80%), RACGP (78%) and NICE (76%). CPGs with the least agreement for pharmacological recommendations were ESCEO and NICE (−0.14), ACR (−0.08), and RACGP (−0.01). The highest agreements were between RACGP and NICE (0.53), RACGP and ACR (0.61), and NICE and ACR (0.91). Decreased internal validity determined by low-quality AGREE scores(<60%) in editorial independence were associated with less agreement for pharmacological recommendations. Conclusion: There were associations between guideline quality and agreement scores. Future guideline development should be informed by robust evidence, editorial independence and methodological rigour to ensure a harmonisation of recommendations. End-users of CPGs must recognise the contextual factors associated with the development of OA CPGs and balance these factors with available evidence.

Electrification to reduce or eliminate greenhouse gas emissions is essential to mitigate climate change. However, a substantial portion of our manufacturing and transportation infrastructure will be difficult to electrify and/or will continue to use carbon as a key component, including areas in aviation, heavy-duty and marine transportation, and the chemical industry. In this Roadmap, we explore how multidisciplinary approaches will enable us to close the carbon cycle and create a circular economy by defossilizing these difficult-to-electrify areas and those that will continue to need carbon. We discuss two approaches for this: developing carbon alternatives and improving our ability to reuse carbon, enabled by separations. Furthermore, we posit that co-design and use-driven fundamental science are essential to reach aggressive greenhouse gas reduction targets.

Natural conversion of ferrihydrite (Fh), a widespread Fe(iii)-oxyhydroxide mineral at the Earth's surface, to thermodynamically more stable iron oxides such as goethite (Gt) and hematite (Hm) is a slow process that spans months to years. Here we examined the effects of synthesis and storage conditions on the hydration, the ratio of tetrahedral to octahedral iron sites, and the transformation of naturally aged 2-line Fh at room temperature and mildly acidic pH over an ageing period of 5 years. Fh samples synthesized and aged in either aerobic or anaerobic conditions were characterized over time by XRD, SEM, thermogravimetric analysis - mass spectroscopy (TGA-MS), and X-ray absorption spectroscopies (XANES and XMCD). The findings show that the ratio of tetrahedral to octahedral Fe(iii) sites in Fh is correlated to its extent of hydration, with fresher Fh samples exhibiting a higher ratio and more bound water. Fresh Fh aged in aerobic conditions has similar bound inorganic carbon, is more hydrated, and has less tetrahedral Fe(iii) than that aged in anaerobic conditions. Hence, for relatively fresh Fh there is a link between Fh properties and storage conditions. However, the long-term ageing characteristics, such as the transformation rate and relative phase fraction of Gt and Hm products, are not noticeably impacted by storage conditions. TGA-MS measurements coupled with O K-edge XANES spectra confirm that Fh tends to lose its hydration as it ages, as expected. Corresponding Fe L2,3-edge XMCD spectra reveal that this dehydration is coupled to a steady decrease in the ratio of tetrahedral to octahedral Fe(iii) sites. In addition to the obvious constraints these findings place on making comparisons across Fh samples of different age and environmental settings, they also highlight that Fh structure, and consequently magnetism, are linked to its bound water content.

In the modern "omics" era, measurement of the human exposome is a critical missing link between genetic drivers and disease outcomes. High-resolution mass spectrometry (HRMS), routinely used in proteomics and metabolomics, has emerged as a leading technology to broadly profile chemical exposure agents and related biomolecules for accurate mass measurement, high sensitivity, rapid data acquisition, and increased resolution of chemical space. Non-targeted approaches are increasingly accessible, supporting a shift from conventional hypothesis-driven, quantitation-centric targeted analyses toward data-driven, hypothesis-generating chemical exposome-wide profiling. However, HRMS-based exposomics encounters unique challenges. New analytical and computational infrastructures are needed to expand the analysis coverage through streamlined, scalable, and harmonized workflows and data pipelines that permit longitudinal chemical exposome tracking, retrospective validation, and multi-omics integration for meaningful health-oriented inferences. In this article, we survey the literature on state-of-the-art HRMS-based technologies, review current analytical workflows and informatic pipelines, and provide an up-to-date reference on exposomic approaches for chemists, toxicologists, epidemiologists, care providers, and stakeholders in health sciences and medicine. We propose efforts to benchmark fit-for-purpose platforms for expanding coverage of chemical space, including gas/liquid chromatography-HRMS (GC-HRMS and LC-HRMS), and discuss opportunities, challenges, and strategies to advance the burgeoning field of the exposome.

In the modern “omics” era, measurement of the human exposome is a critical missing link between genetic drivers and disease outcomes. High-resolution mass spectrometry (HRMS), routinely used in proteomics and metabolomics, has emerged as a leading technology to broadly profile chemical exposure agents and related biomolecules for accurate mass measurement, high sensitivity, rapid data acquisition, and increased resolution of chemical space. Non-targeted approaches are increasingly accessible, supporting a shift from conventional hypothesis-driven, quantitation-centric targeted analyses toward data-driven, hypothesis-generating chemical exposome-wide profiling. However, HRMS-based exposomics encounters unique challenges. New analytical and computational infrastructures are needed to expand the analysis coverage through streamlined, scalable, and harmonized workflows and data pipelines that permit longitudinal chemical exposome tracking, retrospective validation, and multi-omics integration for meaningful health-oriented inferences. In this article, we survey the literature on state-of-the-art HRMS-based technologies, review current analytical workflows and informatic pipelines, and provide an up-to-date reference on exposomic approaches for chemists, toxicologists, epidemiologists, care providers, and stakeholders in health sciences and medicine. We propose efforts to benchmark fit-for-purpose platforms for expanding coverage of chemical space, including gas/liquid chromatography-HRMS (GC-HRMS and LC-HRMS), and discuss opportunities, challenges, and strategies to advance the burgeoning field of the exposome.

An important open problem in supersingular isogeny-based cryptography is to produce, without a trusted authority, concrete examples of ‘hard supersingular curves’ that is equations for supersingular curves for which computing the endomorphism ring is as difficult as it is for random supersingular curves. A related open problem is to produce a hash function to the vertices of the supersingular $ell $-isogeny graph, which does not reveal the endomorphism ring, or a path to a curve of known endomorphism ring. Such a hash function would open up interesting cryptographic applications. In this paper, we document a number of (thus far) failed attempts to solve this problem, in the hope that we may spur further research, and shed light on the challenges and obstacles to this endeavour. The mathematical approaches contained in this article include: (i) iterative root-finding for the supersingular polynomial; (ii) gcd’s of specialized modular polynomials; (iii) using division polynomials to create small systems of equations; (iv) taking random walks in the isogeny graph of abelian surfaces, and applying Kummer surfaces and (v) using quantum random walks., info:eu-repo/semantics/published

This study investigates instances where experienced researchers make explicit claims of lack of knowledge (e.g., ‘I have to reveal my ignorance completely’) in the context of paper discussions during research group meetings. Drawing on multimodal interaction analysis, the analysis focuses on epistemic disclaimers in their sequential contexts, and the local management of institutional identities and domains of knowledge. The analyzed data draw from video-ethnographic work involving participant observation and video recordings at a research program in Physical Chemistry at a Swedish university. Focusing on epistemic disclaimers occurring in first pair-parts,the analysis explores how senior researchers employ disclaimers to mark the eliciting function oftheir questions, as they work to encourage participation by opening the floor while positioning co-participants as (more) knowledgeable. The results evidence how the accomplishment of peer collaboration and knowledge distribution in scientific work involves the management of rights and responsibilities with respect to knowledge and scientific expertise.

Based on ethnographic fieldwork including video recordings, this study examines how pre-teen girls, in everyday peer interaction, deal with controversial issues on a social media network. Drawing on a multimodal interactional approach, we analyze the situated affective and collaborative embodied work through which a peer group unravels and remedies problematic online behaviors (cheating, scamming,stealing). The results show how the girls affectively align themselves to deal with online risks in ways that demand different social media skills as well as networks of supportive peers. The girls agentively draw upon the affordances (texting, images, social norms) of the social media network as well as their expertise and collective knowledge of similar online events. The creative power of the peer group plays an important role, in terms of how the girls nourish their peer culture while building mutual trust and collaborative competencies for interpreting and anticipating risky practices in their lives with peers.

This study explores children’s appropriation of media rules in a group of boys (10 years) in Sweden. The analysis is based on focus-group interviews where rules regulating children’s use of mobile phones in school was discussed. Drawing on ethnomethodology and conversation analysis, the focus is on how rules are made sense of and appropriated, and how this contributes to establishing, negotiating, and sustaining a moral order for digital media use. The findings show that the children justify rules by discussing them in relation to their school context, through criticism of the enforcement of rules, and through navigating different rule systems.

Using the framework of conversation analysis, this paper examines aided-speaking students’ unsolicited speech-generating device(SGD)-mediated questions in teacher-fronted classroom talk. The analysis draws on a corpus of 18 h of video-recorded classroom interactions including 23 aided-speaking students using SGDs or picture-based communication boards. In all, 5% of the students’contributions were unsolicited questions, produced by three students.The students were found to orient to turn transition relevance places, but due to prolonged production time their questions risked sequential and topical misplacement in the ongoing classroomtalk and were vulnerable to misunderstandings. To address this problem, students activated the synthetic voice before finalising the question, claiming the interactional floor while securingtime to complete their utterance. They also refrained from activating the synthetic voice and instead made the question visually available for the teacher to read, thereby transferring the responsibilityfor answering the question to the teacher when sequentially and topically relevant. The study demonstrates the complex interactional process of formulating SGD-mediated questions, sometimes requiring that the teacher, assistants, and students engage in repair work and scaffolding to establish the meaning of a student’s utterance. The findings imply that the treatment of non-speaking students’ contributions as questions requires designated teacher work.

Based on ethnographic fieldwork including video recordings, this study examines how pre-teen girls, in everyday peer interaction, deal with controversial issues on a social media network. Drawing on a multimodal interactional approach, we analyze the situated affective and collaborative embodied work through which a peer group unravels and remedies problematic online behaviors (cheating, scamming,stealing). The results show how the girls affectively align themselves to deal with online risks in ways that demand different social media skills as well as networks of supportive peers. The girls agentively draw upon the affordances (texting, images, social norms) of the social media network as well as their expertise and collective knowledge of similar online events. The creative power of the peer group plays an important role, in terms of how the girls nourish their peer culture while building mutual trust and collaborative competencies for interpreting and anticipating risky practices in their lives with peers.

This study investigates instances where experienced researchers make explicit claims of lack of knowledge (e.g., ‘I have to reveal my ignorance completely’) in the context of paper discussions during research group meetings. Drawing on multimodal interaction analysis, the analysis focuses on epistemic disclaimers in their sequential contexts, and the local management of institutional identities and domains of knowledge. The analyzed data draw from video-ethnographic work involving participant observation and video recordings at a research program in Physical Chemistry at a Swedish university. Focusing on epistemic disclaimers occurring in first pair-parts,the analysis explores how senior researchers employ disclaimers to mark the eliciting function oftheir questions, as they work to encourage participation by opening the floor while positioning co-participants as (more) knowledgeable. The results evidence how the accomplishment of peer collaboration and knowledge distribution in scientific work involves the management of rights and responsibilities with respect to knowledge and scientific expertise.

This study explores children’s appropriation of media rules in a group of boys (10 years) in Sweden. The analysis is based on focus-group interviews where rules regulating children’s use of mobile phones in school was discussed. Drawing on ethnomethodology and conversation analysis, the focus is on how rules are made sense of and appropriated, and how this contributes to establishing, negotiating, and sustaining a moral order for digital media use. The findings show that the children justify rules by discussing them in relation to their school context, through criticism of the enforcement of rules, and through navigating different rule systems.

The use of DEHP (diethylhexyl phthalate) is now banned for most applications in Europe; the exception is for blood bags, where its toxicity is overshadowed by its ability to extend the storage life of red blood cells. Another plasticiser, BTHC (butanoyl trihexyl citrate), is used in paediatric blood bags but does not stabilise blood cells as effectively. Interactions between plasticisers and lipids are investigated with a phospholipid, DMPC, to understand the increased stability of blood cells in the presence of DEHP as well as bioaccumulation and identify differences with BTHC. Mixed monolayers of DMPC and DEHP or BTHC were studied on Langmuir troughs where surface pressure/area isotherms can be measured. Neutron reflection measurements were made to determine the composition and structure of these mixed layers. A large amount of plasticiser can be incorporated into a DMPC monolayer but once an upper limit is reached, plasticiser is selectively removed from the interface at high surface pressures. The upper limit is found to occur between 40-60 mol% for DEHP and 20-40 mol% for BTHC. The areas per molecule are also different with DEHP being in the range of 50-100 Å2 and BTHC being 65-120 Å2. Results indicate that BTHC does not fit as well as DEHP in DMPC monolayers which could help explain the differences observed with regards to the stability of blood cells., We thank SSF for the funding for EG in the SwedNESS graduate school, grant number GSn15-0008. The ISIS Pulsed Neutron and Muon Facility is thanked for the allocations of beam time under RB1810574 (SURF) and RB1820418 (INTER).

The use of DEHP (diethylhexyl phthalate) is now banned for most applications in Europe; the exception is for blood bags, where its toxicity is overshadowed by its ability to extend the storage life of red blood cells. Another plasticiser, BTHC (butanoyl trihexyl citrate), is used in paediatric blood bags but does not stabilise blood cells as effectively. Interactions between plasticisers and lipids are investigated with a phospholipid, DMPC, to understand the increased stability of blood cells in the presence of DEHP as well as bioaccumulation and identify differences with BTHC. Mixed monolayers of DMPC and DEHP or BTHC were studied on Langmuir troughs where surface pressure/area isotherms can be measured. Neutron reflection measurements were made to determine the composition and structure of these mixed layers. A large amount of plasticiser can be incorporated into a DMPC monolayer but once an upper limit is reached, plasticiser is selectively removed from the interface at high surface pressures. The upper limit is found to occur between 40–60 mol% for DEHP and 20–40 mol% for BTHC. The areas per molecule are also different with DEHP being in the range of 50–100 Å2 and BTHC being 65–120 Å2. Results indicate that BTHC does not fit as well as DEHP in DMPC monolayers which could help explain the differences observed with regards to the stability of blood cells.

Based on ethnographic fieldwork including video recordings, this study examines how pre-teen girls, in everyday peer interaction, deal with controversial issues on a social media network. Drawing on a multimodal interactional approach, we analyze the situated affective and collaborative embodied work through which a peer group unravels and remedies problematic online behaviors (cheating, scamming,stealing). The results show how the girls affectively align themselves to deal with online risks in ways that demand different social media skills as well as networks of supportive peers. The girls agentively draw upon the affordances (texting, images, social norms) of the social media network as well as their expertise and collective knowledge of similar online events. The creative power of the peer group plays an important role, in terms of how the girls nourish their peer culture while building mutual trust and collaborative competencies for interpreting and anticipating risky practices in their lives with peers.

This study explores children’s appropriation of media rules in a group of boys (10 years) in Sweden. The analysis is based on focus-group interviews where rules regulating children’s use of mobile phones in school was discussed. Drawing on ethnomethodology and conversation analysis, the focus is on how rules are made sense of and appropriated, and how this contributes to establishing, negotiating, and sustaining a moral order for digital media use. The findings show that the children justify rules by discussing them in relation to their school context, through criticism of the enforcement of rules, and through navigating different rule systems.

This study investigates instances where experienced researchers make explicit claims of lack of knowledge (e.g., ‘I have to reveal my ignorance completely’) in the context of paper discussions during research group meetings. Drawing on multimodal interaction analysis, the analysis focuses on epistemic disclaimers in their sequential contexts, and the local management of institutional identities and domains of knowledge. The analyzed data draw from video-ethnographic work involving participant observation and video recordings at a research program in Physical Chemistry at a Swedish university. Focusing on epistemic disclaimers occurring in first pair-parts,the analysis explores how senior researchers employ disclaimers to mark the eliciting function oftheir questions, as they work to encourage participation by opening the floor while positioning co-participants as (more) knowledgeable. The results evidence how the accomplishment of peer collaboration and knowledge distribution in scientific work involves the management of rights and responsibilities with respect to knowledge and scientific expertise.

Using the framework of conversation analysis, this paper examines aided-speaking students’ unsolicited speech-generating device(SGD)-mediated questions in teacher-fronted classroom talk. The analysis draws on a corpus of 18 h of video-recorded classroom interactions including 23 aided-speaking students using SGDs or picture-based communication boards. In all, 5% of the students’contributions were unsolicited questions, produced by three students.The students were found to orient to turn transition relevance places, but due to prolonged production time their questions risked sequential and topical misplacement in the ongoing classroomtalk and were vulnerable to misunderstandings. To address this problem, students activated the synthetic voice before finalising the question, claiming the interactional floor while securingtime to complete their utterance. They also refrained from activating the synthetic voice and instead made the question visually available for the teacher to read, thereby transferring the responsibilityfor answering the question to the teacher when sequentially and topically relevant. The study demonstrates the complex interactional process of formulating SGD-mediated questions, sometimes requiring that the teacher, assistants, and students engage in repair work and scaffolding to establish the meaning of a student’s utterance. The findings imply that the treatment of non-speaking students’ contributions as questions requires designated teacher work.

The use of DEHP (diethylhexyl phthalate) is now banned for most applications in Europe; the exception is for blood bags, where its toxicity is overshadowed by its ability to extend the storage life of red blood cells. Another plasticiser, BTHC (butanoyl trihexyl citrate), is used in paediatric blood bags but does not stabilise blood cells as effectively. Interactions between plasticisers and lipids are investigated with a phospholipid, DMPC, to understand the increased stability of blood cells in the presence of DEHP as well as bioaccumulation and identify differences with BTHC. Mixed monolayers of DMPC and DEHP or BTHC were studied on Langmuir troughs where surface pressure/area isotherms can be measured. Neutron reflection measurements were made to determine the composition and structure of these mixed layers. A large amount of plasticiser can be incorporated into a DMPC monolayer but once an upper limit is reached, plasticiser is selectively removed from the interface at high surface pressures. The upper limit is found to occur between 40–60 mol% for DEHP and 20–40 mol% for BTHC. The areas per molecule are also different with DEHP being in the range of 50–100 Å2 and BTHC being 65–120 Å2. Results indicate that BTHC does not fit as well as DEHP in DMPC monolayers which could help explain the differences observed with regards to the stability of blood cells.

Aims/hypothesis: The temporal suppression of insulin clearance after glucose ingestion is a key determinant of glucose tolerance for people without type 2 diabetes. Whether similar adaptations are observed after the ingestion of a mixed-macronutrient meal is unclear. Methods: In a secondary analysis of data derived from two randomised, controlled trials, we studied the temporal responses of insulin clearance after the ingestion of a standardised breakfast meal consisting of cereal and milk in lean normoglycaemic individuals (n=12; Lean-NGT), normoglycaemic individuals with central obesity (n=11; Obese-NGT) and in people with type 2 diabetes (n=19). Pre-hepatic insulin secretion rates were determined by the deconvolution of C-peptide, and insulin clearance was calculated using a single-pool model. Insulin sensitivity was measured by an oral minimal model. Results: There were divergent time course changes in insulin clearance between groups. In the Lean-NGT group, there was an immediate post-meal increase in insulin clearance compared with pre-meal values (p<0.05), whereas insulin clearance remained stable at baseline values in Obese-NGT or declined slightly in the type 2 diabetes group (p<0.05). The mean AUC for insulin clearance during the test was ~40% lower in the Obese-NGT (1.3 ± 0.4 l min−1 m−2) and type 2 diabetes (1.4 ± 0.7 l min−1 m−2) groups compared with Lean-NGT (1.9 ± 0.5 l min−1 m−2; p<0.01), with no difference between the Obese-NGT and type 2 diabetes groups. HOMA-IR and glucagon AUC emerged as predictors of insulin clearance AUC, independent of BMI, age or insulin sensitivity (adjusted R2=0.670). Individuals with increased glucagon AUC had a 40% reduction in insulin clearance AUC (~ −0.75 l min−1 m−2; p<0.001). Conclusions/interpretation: The ingestion of a mixed-macronutrient meal augments differing temporal profiles in insulin clearance among individuals without type 2 diabetes, which is associated with HOMA-IR and the secretion of glucagon. Further

This study investigates instances where experienced researchers make explicit claims of lack of knowledge (e.g., ‘I have to reveal my ignorance completely’) in the context of paper discussions during research group meetings. Drawing on multimodal interaction analysis, the analysis focuses on epistemic disclaimers in their sequential contexts, and the local management of institutional identities and domains of knowledge. The analyzed data draw from video-ethnographic work involving participant observation and video recordings at a research program in Physical Chemistry at a Swedish university. Focusing on epistemic disclaimers occurring in first pair-parts,the analysis explores how senior researchers employ disclaimers to mark the eliciting function oftheir questions, as they work to encourage participation by opening the floor while positioning co-participants as (more) knowledgeable. The results evidence how the accomplishment of peer collaboration and knowledge distribution in scientific work involves the management of rights and responsibilities with respect to knowledge and scientific expertise.

Using the framework of conversation analysis, this paper examines aided-speaking students’ unsolicited speech-generating device(SGD)-mediated questions in teacher-fronted classroom talk. The analysis draws on a corpus of 18 h of video-recorded classroom interactions including 23 aided-speaking students using SGDs or picture-based communication boards. In all, 5% of the students’contributions were unsolicited questions, produced by three students.The students were found to orient to turn transition relevance places, but due to prolonged production time their questions risked sequential and topical misplacement in the ongoing classroomtalk and were vulnerable to misunderstandings. To address this problem, students activated the synthetic voice before finalising the question, claiming the interactional floor while securingtime to complete their utterance. They also refrained from activating the synthetic voice and instead made the question visually available for the teacher to read, thereby transferring the responsibilityfor answering the question to the teacher when sequentially and topically relevant. The study demonstrates the complex interactional process of formulating SGD-mediated questions, sometimes requiring that the teacher, assistants, and students engage in repair work and scaffolding to establish the meaning of a student’s utterance. The findings imply that the treatment of non-speaking students’ contributions as questions requires designated teacher work.

Based on ethnographic fieldwork including video recordings, this study examines how pre-teen girls, in everyday peer interaction, deal with controversial issues on a social media network. Drawing on a multimodal interactional approach, we analyze the situated affective and collaborative embodied work through which a peer group unravels and remedies problematic online behaviors (cheating, scamming,stealing). The results show how the girls affectively align themselves to deal with online risks in ways that demand different social media skills as well as networks of supportive peers. The girls agentively draw upon the affordances (texting, images, social norms) of the social media network as well as their expertise and collective knowledge of similar online events. The creative power of the peer group plays an important role, in terms of how the girls nourish their peer culture while building mutual trust and collaborative competencies for interpreting and anticipating risky practices in their lives with peers.

This study explores children’s appropriation of media rules in a group of boys (10 years) in Sweden. The analysis is based on focus-group interviews where rules regulating children’s use of mobile phones in school was discussed. Drawing on ethnomethodology and conversation analysis, the focus is on how rules are made sense of and appropriated, and how this contributes to establishing, negotiating, and sustaining a moral order for digital media use. The findings show that the children justify rules by discussing them in relation to their school context, through criticism of the enforcement of rules, and through navigating different rule systems.

The use of DEHP (diethylhexyl phthalate) is now banned for most applications in Europe; the exception is for blood bags, where its toxicity is overshadowed by its ability to extend the storage life of red blood cells. Another plasticiser, BTHC (butanoyl trihexyl citrate), is used in paediatric blood bags but does not stabilise blood cells as effectively. Interactions between plasticisers and lipids are investigated with a phospholipid, DMPC, to understand the increased stability of blood cells in the presence of DEHP as well as bioaccumulation and identify differences with BTHC. Mixed monolayers of DMPC and DEHP or BTHC were studied on Langmuir troughs where surface pressure/area isotherms can be measured. Neutron reflection measurements were made to determine the composition and structure of these mixed layers. A large amount of plasticiser can be incorporated into a DMPC monolayer but once an upper limit is reached, plasticiser is selectively removed from the interface at high surface pressures. The upper limit is found to occur between 40-60 mol% for DEHP and 20-40 mol% for BTHC. The areas per molecule are also different with DEHP being in the range of 50-100 Å2 and BTHC being 65-120 Å2. Results indicate that BTHC does not fit as well as DEHP in DMPC monolayers which could help explain the differences observed with regards to the stability of blood cells., We thank SSF for the funding for EG in the SwedNESS graduate school, grant number GSn15-0008. The ISIS Pulsed Neutron and Muon Facility is thanked for the allocations of beam time under RB1810574 (SURF) and RB1820418 (INTER).

The use of DEHP (diethylhexyl phthalate) is now banned for most applications in Europe; the exception is for blood bags, where its toxicity is overshadowed by its ability to extend the storage life of red blood cells. Another plasticiser, BTHC (butanoyl trihexyl citrate), is used in paediatric blood bags but does not stabilise blood cells as effectively. Interactions between plasticisers and lipids are investigated with a phospholipid, DMPC, to understand the increased stability of blood cells in the presence of DEHP as well as bioaccumulation and identify differences with BTHC. Mixed monolayers of DMPC and DEHP or BTHC were studied on Langmuir troughs where surface pressure/area isotherms can be measured. Neutron reflection measurements were made to determine the composition and structure of these mixed layers. A large amount of plasticiser can be incorporated into a DMPC monolayer but once an upper limit is reached, plasticiser is selectively removed from the interface at high surface pressures. The upper limit is found to occur between 40–60 mol% for DEHP and 20–40 mol% for BTHC. The areas per molecule are also different with DEHP being in the range of 50–100 Å2 and BTHC being 65–120 Å2. Results indicate that BTHC does not fit as well as DEHP in DMPC monolayers which could help explain the differences observed with regards to the stability of blood cells.

Single-cell RNA sequencing (scRNA-Seq) has emerged as a powerful tool for understanding cellular heterogeneity and function. However the choice of sample multiplexing reagents can impact data quality and experimental outcomes. In this study, we compared various multiplexing reagents, including MULTI-Seq, Hashtag antibody, and CellPlex, across diverse sample types such as human peripheral blood mononuclear cells (PBMCs), mouse embryonic brain and patient-derived xenografts (PDXs). We found that all multiplexing reagents worked well in cell types robust to ex vivo manipulation but suffered from signal-to-noise issues in more delicate sample types. We compared multiple demultiplexing algorithms which differed in performance depending on data quality. We find that minor improvements to laboratory workflows such as titration and rapid processing are critical to optimal performance. We also compared the performance of fixed scRNA-Seq kits and highlight the advantages of the Parse Biosciences kit for fragile samples. Highly multiplexed scRNA-Seq experiments require more sequencing resources, therefore we evaluated CRISPR-based destruction of non-informative genes to enhance sequencing value. Our comprehensive analysis provides insights into the selection of appropriate sample multiplexing reagents and protocols for scRNA-Seq experiments, facilitating more accurate and cost-effective studies.

OBJECTIVES: This study examined the factorial invariance of the factor structure of the Wechsler Intelligence Scale for Children, Fifth Edition (WISC-V) across the UK, US and Australia & New Zealand (A&NZ). The factorial equivalence of cognitive assessments should be demonstrated before assuming cross-culture generalizability and interpretations of score comparisons. METHODS: Data were obtained from the UK, US and A&NZ normative standardizations of the WISC-V. The samples consisted of 415 UK, 2200 US and 528 A&NZ children, aged 6-16. Confirmatory factor analysis was applied separately in each sample to establish the baseline model. Next, tests of factorial invariance were undertaken using the recommended hierarchical approach, firstly across the UK and A&NZ samples and then across the UK and US samples. RESULTS: The five-factor first-order scoring model was found to be excellent fit across all three samples independently. Strict factorial invariance of the WISC-V was demonstrated firstly across the UK and A&NZ and secondly the UK and US nationally representative standardization samples. Comparison of latent means found small but significant differences in female children across the UK and A&NZ samples. CONCLUSIONS: Consistent with previous research, these results demonstrate the generality of the WISC-V factor structure across the UK, US and A&NZ. Furthermore, as the WISC-V factor structure aligns with the Cattell-Horn-Carroll (CHC) model of cognitive abilities, the results add further support to the cross-cultural generalizability of the CHC model. Small but significant differences in latent factor scores found across samples support the development and use of local normative data.

INTRODUCTION: As people increasingly migrate to online shopping platforms, hard-won improvements in requirements for consumer information provision at the point of sale are being eroded. An example is the alcohol pregnancy warning label for packaged alcoholic beverages that has been recently introduced in Australia and New Zealand. The aim of the present study was to assess the extent to which the pregnancy warning was visible at the online point of sale when the requirement became mandatory in August 2023. METHODS: Data for alcohol products sold on the websites of the two largest alcohol retailers in Australia were web-scraped from 1 to 3 August 2023. The captured data for 8343 alcoholic beverages were inspected to determine whether the pregnancy warning was visible. RESULTS: Virtually no products (0.1%) had the mandatory warning visible on the main sales page, and only 7% enabled visibility of the warning via optional product image rotation functionality. DISCUSSION AND CONCLUSIONS: The almost complete absence of the mandatory pregnancy warnings on the main product pages of major alcohol retailers' websites highlights the regulatory problems posed by the emerging shift to online shopping. The very low prevalence of visible pregnancy warnings is likely to be an overestimate of the extent to which consumers would be exposed to warnings due to images being counted as being present regardless of their quality or readability. New regulation is needed to ensure that mandatory information requirements for harmful products are applied to online shopping contexts.

BACKGROUND AND AIMS: Accurate biomarkers to predict outcomes following discontinuation of nucleos(t)ide analogue (NA) therapy are needed. We evaluated serum hepatitis B core-related antigen (HBcrAg) level as a biomarker for predicting outcomes after NA discontinuation. METHODS: Patients with HBeAg-negative chronic hepatitis B (CHB) without cirrhosis were enrolled in a prospective trial evaluating clinical outcomes until 96 weeks after NA discontinuation. End of treatment (EOT) and off-treatment levels of serum HBcrAg, HBsAg, HBV RNA and HBV DNA were used to predict key clinical outcomes including hepatitis flare (ALT ≥5 × ULN and HBV DNA > 2000 IU/mL). The SCALE-B score was calculated for the purposes of model validation. RESULTS: HBcrAg was tested amongst 65 participants. The median age was 54 years, 54% were male and 83% were Asian. HBcrAg was detectable in 86% patients. HBcrAg level ≥4 log U/mL at EOT was predictive of hepatitis flare [8/10 (80%) vs. 17/55 (31%), p = .001]. The presence of either HBcrAg ≥4 log U/mL or detectable HBV RNA at EOT predicted for both biochemical relapse and hepatitis flare. The SCALE-B model at EOT predicted for virological relapse, biochemical relapse, hepatitis flare and HBsAg loss in this cohort. An increase in the serum HBcrAg level off-treatment was also associated with hepatitis flare. No participant with EOT HBcrAg level ≥4 log U/mL achieved HBsAg loss. CONCLUSIONS: High levels of serum HBcrAg predict for hepatitis flare after stopping NA therapy and low likelihood of HBsAg loss at week 96. People with high levels of serum HBcrAg are not suitable candidates for NA discontinuation.

uses the pit village dialect of North-East England (Pitmatic) in the dialogue of working-class characters. The accompanying exegesis explores how writers of contemporary fiction from the UK use dialect to represent Northern and working-class characters, and whether this choice affects the response to this work from the book industry in the form of acceptance for publication, reviews and sales.

This study explores children’s appropriation of media rules in a group of boys (10 years) in Sweden. The analysis is based on focus-group interviews where rules regulating children’s use of mobile phones in school was discussed. Drawing on ethnomethodology and conversation analysis, the focus is on how rules are made sense of and appropriated, and how this contributes to establishing, negotiating, and sustaining a moral order for digital media use. The findings show that the children justify rules by discussing them in relation to their school context, through criticism of the enforcement of rules, and through navigating different rule systems.

This study investigates instances where experienced researchers make explicit claims of lack of knowledge (e.g., ‘I have to reveal my ignorance completely’) in the context of paper discussions during research group meetings. Drawing on multimodal interaction analysis, the analysis focuses on epistemic disclaimers in their sequential contexts, and the local management of institutional identities and domains of knowledge. The analyzed data draw from video-ethnographic work involving participant observation and video recordings at a research program in Physical Chemistry at a Swedish university. Focusing on epistemic disclaimers occurring in first pair-parts,the analysis explores how senior researchers employ disclaimers to mark the eliciting function oftheir questions, as they work to encourage participation by opening the floor while positioning co-participants as (more) knowledgeable. The results evidence how the accomplishment of peer collaboration and knowledge distribution in scientific work involves the management of rights and responsibilities with respect to knowledge and scientific expertise.

Based on ethnographic fieldwork including video recordings, this study examines how pre-teen girls, in everyday peer interaction, deal with controversial issues on a social media network. Drawing on a multimodal interactional approach, we analyze the situated affective and collaborative embodied work through which a peer group unravels and remedies problematic online behaviors (cheating, scamming,stealing). The results show how the girls affectively align themselves to deal with online risks in ways that demand different social media skills as well as networks of supportive peers. The girls agentively draw upon the affordances (texting, images, social norms) of the social media network as well as their expertise and collective knowledge of similar online events. The creative power of the peer group plays an important role, in terms of how the girls nourish their peer culture while building mutual trust and collaborative competencies for interpreting and anticipating risky practices in their lives with peers.

Using the framework of conversation analysis, this paper examines aided-speaking students’ unsolicited speech-generating device(SGD)-mediated questions in teacher-fronted classroom talk. The analysis draws on a corpus of 18 h of video-recorded classroom interactions including 23 aided-speaking students using SGDs or picture-based communication boards. In all, 5% of the students’contributions were unsolicited questions, produced by three students.The students were found to orient to turn transition relevance places, but due to prolonged production time their questions risked sequential and topical misplacement in the ongoing classroomtalk and were vulnerable to misunderstandings. To address this problem, students activated the synthetic voice before finalising the question, claiming the interactional floor while securingtime to complete their utterance. They also refrained from activating the synthetic voice and instead made the question visually available for the teacher to read, thereby transferring the responsibilityfor answering the question to the teacher when sequentially and topically relevant. The study demonstrates the complex interactional process of formulating SGD-mediated questions, sometimes requiring that the teacher, assistants, and students engage in repair work and scaffolding to establish the meaning of a student’s utterance. The findings imply that the treatment of non-speaking students’ contributions as questions requires designated teacher work.

The use of DEHP (diethylhexyl phthalate) is now banned for most applications in Europe; the exception is for blood bags, where its toxicity is overshadowed by its ability to extend the storage life of red blood cells. Another plasticiser, BTHC (butanoyl trihexyl citrate), is used in paediatric blood bags but does not stabilise blood cells as effectively. Interactions between plasticisers and lipids are investigated with a phospholipid, DMPC, to understand the increased stability of blood cells in the presence of DEHP as well as bioaccumulation and identify differences with BTHC. Mixed monolayers of DMPC and DEHP or BTHC were studied on Langmuir troughs where surface pressure/area isotherms can be measured. Neutron reflection measurements were made to determine the composition and structure of these mixed layers. A large amount of plasticiser can be incorporated into a DMPC monolayer but once an upper limit is reached, plasticiser is selectively removed from the interface at high surface pressures. The upper limit is found to occur between 40–60 mol% for DEHP and 20–40 mol% for BTHC. The areas per molecule are also different with DEHP being in the range of 50–100 Å2 and BTHC being 65–120 Å2. Results indicate that BTHC does not fit as well as DEHP in DMPC monolayers which could help explain the differences observed with regards to the stability of blood cells.

Using the framework of conversation analysis, this paper examinesaided-speaking students’ unsolicited speech-generating device(SGD)-mediated questions in teacher-fronted classroom talk. Theanalysis draws on a corpus of 18 h of video-recorded classroominteractions including 23 aided-speaking students using SGDs orpicture-based communication boards. In all, 5% of the students’contributions were unsolicited questions, produced by three students.The students were found to orient to turn transition relevanceplaces, but due to prolonged production time their questionsrisked sequential and topical misplacement in the ongoing classroomtalk and were vulnerable to misunderstandings. To addressthis problem, students activated the synthetic voice before finalisingthe question, claiming the interactional floor while securingtime to complete their utterance. They also refrained from activatingthe synthetic voice and instead made the question visuallyavailable for the teacher to read, thereby transferring the responsibilityfor answering the question to the teacher when sequentiallyand topically relevant. The study demonstrates the complex interactionalprocess of formulating SGD-mediated questions, sometimesrequiring that the teacher, assistants, and students engagein repair work and scaffolding to establish the meaning ofa student’s utterance. The findings imply that the treatment of nonspeakingstudents’ contributions as questions requires designatedteacher work.

Background Multiple long-term conditions—the co-existence of two or more chronic health conditions in an individual—present an increasing challenge to populations and healthcare systems worldwide. This challenge is keenly felt in hospital settings where care is oriented around specialist provision for single conditions. The aim of this scoping review was to identify and summarise published qualitative research on the experiences of hospital care for people living with multiple long-term conditions, their informal caregivers and healthcare professionals. Methods We undertook a scoping review, following established guidelines, of primary qualitative research on experiences of hospital care for people living with multiple long-term conditions published in peer-reviewed journals between Jan 2010 and June 2022. We conducted systematic electronic searches of MEDLINE, CINAHL, PsycInfo, Proquest Social Science Premium, Web of Science, Scopus and Embase, supplemented by citation tracking. Studies were selected for inclusion by two reviewers using an independent screening process. Data extraction included study populations, study design, findings and author conclusions. We took a narrative approach to reporting the findings. Results Of 8002 titles and abstracts screened, 54 papers reporting findings from 41 studies conducted in 14 countries were identified as eligible for inclusion. The perspectives of people living with multiple long-term conditions (21 studies), informal caregivers (n = 13) and healthcare professionals (n = 27) were represented, with 15 studies reporting experiences of more than one group. Findings included poor service integration and lack of person-centred care, limited confidence of healthcare professionals to treat conditions outside of their specialty, and time pressures leading to hurried care transitions. Few studies explored inequities in experiences of hospital care. Conclusions Qualitative research evidence on the experiences of hospital care for

Objective: To investigate speech development of children aged 5 and 10 years with repaired unilateral cleft lip and palate (UCLP) and identify speech characteristics when speech proficiency is not at 'peer level' at 10 years. Estimate how the number of speech therapy visits are related to speech proficiency at 10 years, and what factors are predictive of whether a child's speech proficiency at 10 years is at 'peer level' or not. Design: Longitudinal complete datasets from the Scandcleft project Participants: 320 children from nine cleft palate teams in five countries, operated on with one out of four surgical methods. Interventions: Secondary velopharyngeal surgery (VP-surgery) and number of speech therapy visits (ST-visits), a proxy for speech intervention. Main Outcome Measures: 'Peer level' of percentage of consonants correct (PCC, > 91%) and the composite score of velopharyngeal competence (VPC-Sum, 0-1). Results: Speech proficiency improved, with only 23% of the participants at 'peer level' at 5 years, compared to 56% at 10 years. A poorer PCC score was the most sensitive marker for the 44% below 'peer level' at 10-year-of-age. The best predictor of 'peer level' speech proficiency at 10 years was speech proficiency at 5 years. A high number of ST-visits received did not improve the probability of achieving 'peer level' speech, and many children seemed to have received excessive amounts of ST-visits without substantial improvement. Conclusions: It is important to strive for speech at 'peer level' before age 5. Criteria for speech therapy intervention and for methods used needs to be evidence-based.

The rapid expansion of wild pigs (Sus scrofa) throughout the United States has been fueled by unlawful introductions, with invasive populations causing extensive crop losses, damaging native ecosystems, and serving as a reservoir for disease. Multiple states have passed laws prohibiting the possession or transport of wild pigs. However, genetic and phenotypic similarities between domestic pigs and invasive wild pigs—which overwhelmingly represent domestic pig and wild boar hybrids—pose a challenge for the enforcement of such regulations. We sought to exploit wild boar ancestry as a common attribute among the vast majority of invasive wild pigs as a means of genetically differentiating wild pigs from breeds of domestic pig found within the United States. We organized reference high-density single nucleotide polymorphism genotypes (1039 samples from 33 domestic breeds and 382 samples from 16 wild boar populations) into five genetically cohesive reference groups: mixed-commercial breeds, Durocs, heritage breeds, primitive breeds, and wild boar. Building upon well-established genetic clustering approaches, we structured the test statistic to describe the difference in the likelihood of a given genotype's ancestry vectors (sensu genetic clustering analysis) if derived strictly from the four described domestic pig reference groups versus allowing for admixture from the wild boar group. By fitting statistical distributions to test statistics of reference domestic pigs, we characterized the distribution of the null hypothesis that a given genotype descends strictly from domestic pig reference groups. We tested the approach with simulated genotypes and empirical data from an additional 29 breeds of domestic pig represented by 435 unique genotypes; all associated test statistics for simulated and empirical domestic pig challenge sets fell within the distribution of reference domestic pigs. We then evaluated 6566 invasive wild pigs sampled across the contiguous United States